FastQCFastQC Report
Thu 26 May 2016
SRR1567913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12043456
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT130980.10875615770091242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT43450.031.9171941
GGCGCGT76500.031.0413195
GGCGATC49350.028.5825462
GCGATCT51100.026.8601573
TGGCGCG87450.026.8286444
GCGCGTG93200.025.5301536
CGCGTGC92700.025.4629237
CGGTGGC99300.024.535771
CGATCTG57300.024.0367074
GTGGCGC102000.023.1878643
GCGTGCC101200.023.0895888
GGTGGCG104600.022.566092
CGTGCCT107300.021.9097339
GCGTTCG41950.017.9472344-45
GTGCGTT42350.017.49607742-43
AAGTGCG43800.017.13289640-41
CTCGCTA40700.016.4534261
AGTGTCG47300.015.86730952-53
GCGACGC20950.015.6421581
ACCGCGT26300.015.349528
Produced by FastQC (version 0.11.5)