##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567913_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12043456 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.02718239681367 31.0 31.0 33.0 28.0 34.0 2 30.751024954963093 31.0 31.0 33.0 27.0 34.0 3 31.002350072935876 31.0 31.0 34.0 27.0 34.0 4 34.78551098621525 37.0 35.0 37.0 32.0 37.0 5 34.760261091168516 37.0 35.0 37.0 32.0 37.0 6 34.67618049171268 37.0 35.0 37.0 31.0 37.0 7 34.64583861974503 37.0 35.0 37.0 31.0 37.0 8 34.75399220954517 37.0 35.0 37.0 32.0 37.0 9 36.30787873514048 39.0 35.0 39.0 32.0 39.0 10-11 36.43625849590018 39.0 36.0 39.0 32.0 39.0 12-13 36.37441163068142 39.0 36.0 39.0 32.0 39.0 14-15 37.389475828200815 39.0 37.0 41.0 32.0 41.0 16-17 37.431886245941364 39.0 37.0 41.0 32.0 41.0 18-19 37.33396128154576 39.0 37.0 41.0 32.0 41.0 20-21 37.33601081782505 39.0 37.0 41.0 31.5 41.0 22-23 37.220734397169714 39.0 36.5 41.0 31.0 41.0 24-25 36.9770319250554 39.0 36.0 41.0 30.5 41.0 26-27 36.84034997927505 39.0 36.0 41.0 30.0 41.0 28-29 36.94570466317974 39.0 36.0 41.0 30.0 41.0 30-31 36.7848154217527 39.0 36.0 41.0 30.0 41.0 32-33 36.75641128260858 39.0 36.0 40.0 30.0 41.0 34-35 36.58545449910723 39.0 35.5 40.0 30.0 41.0 36-37 36.38162749961472 39.0 35.0 40.0 30.0 41.0 38-39 36.228659780049846 38.0 35.0 40.0 29.0 41.0 40-41 36.24613723834753 38.5 35.0 40.0 29.5 41.0 42-43 36.447317364716575 39.0 35.0 40.0 30.0 41.0 44-45 36.43343509537462 39.0 35.0 40.0 30.0 41.0 46-47 36.463397425124484 39.0 35.0 40.0 30.0 41.0 48-49 36.273868148810436 38.5 35.0 40.0 29.0 41.0 50-51 35.889992997026766 38.0 34.5 40.0 28.5 41.0 52-53 35.803823960497716 38.0 34.0 40.0 28.5 41.0 54-55 35.89685207468686 38.0 34.0 40.0 28.0 41.0 56-57 35.706778643937426 38.0 34.0 40.0 28.0 41.0 58-59 35.34299892821463 37.5 34.0 40.0 27.5 41.0 60-61 35.228030683219174 37.0 34.0 40.0 28.0 41.0 62-63 35.02316585870368 37.0 34.0 40.0 27.5 41.0 64-65 34.746162106624546 36.0 34.0 40.0 27.0 41.0 66-67 34.45883108635927 36.0 33.0 39.0 26.0 41.0 68-69 34.20759381692431 35.0 33.0 39.0 26.0 41.0 70-71 33.971958132283625 35.0 33.0 39.0 26.0 40.5 72-73 33.74809112102041 35.0 33.0 38.0 26.0 40.0 74-75 33.42741223947677 35.0 33.0 37.0 26.0 39.5 76-77 33.01313900262516 35.0 33.0 37.0 26.0 39.0 78-79 32.728152035429034 35.0 33.0 36.0 26.0 39.0 80-81 32.42046531327885 35.0 32.0 36.0 25.0 38.0 82-83 32.134322199541394 35.0 32.0 35.5 25.0 37.0 84-85 31.84555031379697 35.0 32.0 35.0 24.0 37.0 86-87 31.638189652538273 35.0 32.0 35.0 24.0 36.5 88-89 31.368044687504984 35.0 32.0 35.0 24.0 36.0 90-91 31.128990714957567 35.0 32.0 35.0 23.0 36.0 92-93 30.908605719155695 35.0 31.0 35.0 21.5 35.5 94-95 30.698546870599273 34.5 31.0 35.0 20.0 35.0 96-97 30.441355205681823 34.0 31.0 35.0 18.5 35.0 98-99 30.133024606890245 34.0 31.0 35.0 12.0 35.0 100-101 29.672749084648128 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2525696203406902 1101 2 0.767620905954189 1101 3 0.7557723455453207 1101 4 0.7709801924052471 1101 5 0.5964386264571075 1101 6 0.4935588868584233 1101 7 0.48662685780181647 1101 8 0.5818529073335057 1101 9 0.6307187642217187 1101 10-11 0.8292480242834088 1101 12-13 0.8547465595757515 1101 14-15 0.8739146126691892 1101 16-17 0.8440300909641323 1101 18-19 0.9203968614392934 1101 20-21 0.8834158494219864 1101 22-23 0.8837880577334545 1101 24-25 1.0182917314300255 1101 26-27 1.0322986324067713 1101 28-29 0.9458482109156705 1101 30-31 0.9893385945129225 1101 32-33 0.9419147685599896 1101 34-35 0.9905094832200874 1101 36-37 0.9877185092625211 1101 38-39 0.8279301112038482 1101 40-41 0.9246774415545858 1101 42-43 1.0644353404497124 1101 44-45 1.0446630894222864 1101 46-47 0.943955767329534 1101 48-49 0.9842764345131556 1101 50-51 0.9448680720846738 1101 52-53 1.0244036884528498 1101 54-55 1.0858866348099383 1101 56-57 1.1499337191907557 1101 58-59 1.292345743511781 1101 60-61 1.1889475042225754 1101 62-63 1.2063010238144756 1101 64-65 1.2554766453701873 1101 66-67 1.1189984125520525 1101 68-69 1.0454913280298115 1101 70-71 0.9316294237065179 1101 72-73 0.8294057423729271 1101 74-75 0.8103668320525159 1101 76-77 0.8508836567236315 1101 78-79 0.8248732848572047 1101 80-81 0.8652373484149294 1101 82-83 0.8370174549183531 1101 84-85 0.9120539960057314 1101 86-87 0.8606816572985672 1101 88-89 0.8899616401607453 1101 90-91 0.8284340334264719 1101 92-93 0.887094483258938 1101 94-95 0.8546668594912674 1101 96-97 0.9438944654764825 1101 98-99 1.0075276804318953 1101 100-101 0.962182809930539 1102 1 0.43672707287357326 1102 2 0.8426517821641895 1102 3 0.8448155618729594 1102 4 0.7598547864778595 1102 5 0.6357447300680761 1102 6 0.5887158021927519 1102 7 0.6811334488801606 1102 8 0.657703750265469 1102 9 0.6887165651834763 1102 10-11 0.853977709672705 1102 12-13 0.8609768692419877 1102 14-15 0.8991822549294639 1102 16-17 0.8024054548443189 1102 18-19 0.9155098091491283 1102 20-21 0.8452381356273193 1102 22-23 1.0026395394653065 1102 24-25 1.140988948541839 1102 26-27 1.0743976374191178 1102 28-29 0.9846928990281043 1102 30-31 1.004408207165561 1102 32-33 0.9180555320842316 1102 34-35 0.8951570615207984 1102 36-37 0.8860508010772818 1102 38-39 0.8656207974598757 1102 40-41 0.9016804138269592 1102 42-43 0.9407148645265977 1102 44-45 1.042575767924184 1102 46-47 0.7806412072465463 1102 48-49 0.8830920407731924 1102 50-51 0.9126566340762494 1102 52-53 0.9579338485851849 1102 54-55 0.9123229168549756 1102 56-57 0.8744986003595869 1102 58-59 1.1061997510870256 1102 60-61 1.0937396613809724 1102 62-63 1.0924230814332319 1102 64-65 1.2107485758928291 1102 66-67 1.2394091252087307 1102 68-69 1.1671146316701666 1102 70-71 1.0153750970151307 1102 72-73 0.9662374465945405 1102 74-75 1.0207812093470991 1102 76-77 1.1487187736421305 1102 78-79 1.1013543307110467 1102 80-81 1.0747880194961041 1102 82-83 1.1028686782849775 1102 84-85 1.105221467128807 1102 86-87 1.1023634708584105 1102 88-89 1.1658123370875622 1102 90-91 1.2239155127239663 1102 92-93 1.251124698512779 1102 94-95 1.2960609662618552 1102 96-97 1.3699675807104335 1102 98-99 1.3892519079389736 1102 100-101 1.4312634782205471 1103 1 0.2859695078265716 1103 2 0.7281079727629631 1103 3 0.5913247806479482 1103 4 0.5501760871847026 1103 5 0.5598075349177947 1103 6 0.46628560101694916 1103 7 0.38007905097448713 1103 8 0.48631932651304055 1103 9 0.5041866311783636 1103 10-11 0.615429134494299 1103 12-13 0.610710194300637 1103 14-15 0.6561648639556026 1103 16-17 0.65031812302405 1103 18-19 0.6311705906917879 1103 20-21 0.621416704282062 1103 22-23 0.7111468630194508 1103 24-25 0.9533038353895122 1103 26-27 0.8951663806335901 1103 28-29 0.7824530975911443 1103 30-31 0.6826752276606385 1103 32-33 0.7527160677732994 1103 34-35 0.8249771642651993 1103 36-37 0.8700595768935031 1103 38-39 0.7931408288351278 1103 40-41 0.6978044191435728 1103 42-43 0.7843968313405867 1103 44-45 0.8391992195653017 1103 46-47 0.6699660940373064 1103 48-49 0.753766693121726 1103 50-51 0.6586494960598515 1103 52-53 0.7213846377494804 1103 54-55 0.7576767706695833 1103 56-57 0.7226885928517746 1103 58-59 0.8949998809359059 1103 60-61 0.8518545280682943 1103 62-63 0.8959843780198753 1103 64-65 0.9632105085223444 1103 66-67 0.916026879436302 1103 68-69 0.8235601818947131 1103 70-71 0.7939552273817938 1103 72-73 0.7322038008245713 1103 74-75 0.7811308282005953 1103 76-77 0.8528060940785878 1103 78-79 0.8558637242826066 1103 80-81 0.8153856438417435 1103 82-83 0.7827100981325756 1103 84-85 0.8253476676847384 1103 86-87 0.7894394675247831 1103 88-89 0.8687797568055338 1103 90-91 0.9536594816454205 1103 92-93 1.0194756397243872 1103 94-95 0.8809473237013847 1103 96-97 0.9639270003637748 1103 98-99 0.968657805230194 1103 100-101 1.084852288742713 1104 1 0.19608163948206325 1104 2 0.5172301040485578 1104 3 0.4254570792085097 1104 4 0.3018661160538585 1104 5 0.23480570513620336 1104 6 0.1398969492990787 1104 7 0.3009376074617265 1104 8 0.29472638078860314 1104 9 0.24183895236451747 1104 10-11 0.3194333150279789 1104 12-13 0.22995913001489043 1104 14-15 0.32882053592207683 1104 16-17 0.17752086708051706 1104 18-19 0.2362437101180106 1104 20-21 0.2912848369142509 1104 22-23 0.274749916796452 1104 24-25 0.434460472856081 1104 26-27 0.3852421429712223 1104 28-29 0.318666854267299 1104 30-31 0.16653970006209562 1104 32-33 0.4211185818912071 1104 34-35 0.5069818991277444 1104 36-37 0.4463347463031013 1104 38-39 0.42712634558107254 1104 40-41 0.6123442058240514 1104 42-43 0.5169583042374626 1104 44-45 0.554706170712663 1104 46-47 0.4405280791092352 1104 48-49 0.6061287195000702 1104 50-51 0.38569634114815443 1104 52-53 0.5692892418329123 1104 54-55 0.5122353420364405 1104 56-57 0.5279324010857991 1104 58-59 0.7775332535547008 1104 60-61 0.7117885488774292 1104 62-63 0.6627776604531803 1104 64-65 0.6395167968126856 1104 66-67 0.5263390036011444 1104 68-69 0.6386445266597889 1104 70-71 0.6138874580753253 1104 72-73 0.46396620625164076 1104 74-75 0.3530175627866825 1104 76-77 0.5404984550551077 1104 78-79 0.4943715976621448 1104 80-81 0.600407744726212 1104 82-83 0.7264619443129021 1104 84-85 0.7135112024830192 1104 86-87 0.6595311597093705 1104 88-89 0.6680100325951166 1104 90-91 0.6433818563927964 1104 92-93 0.5742671157183068 1104 94-95 0.7045532950540547 1104 96-97 0.8432871687199359 1104 98-99 0.9142521425065091 1104 100-101 0.9353703691930164 1105 1 0.2849003503960894 1105 2 0.7012689919491599 1105 3 0.4552419770810765 1105 4 0.37846707491850395 1105 5 0.3843198124924285 1105 6 0.3865503389971039 1105 7 0.4865765927158492 1105 8 0.5279860596218242 1105 9 0.4959773483583092 1105 10-11 0.4550136176027806 1105 12-13 0.4661491678726364 1105 14-15 0.5148811162930471 1105 16-17 0.48892964229973757 1105 18-19 0.49876496296882067 1105 20-21 0.4457628227847934 1105 22-23 0.5065717875776912 1105 24-25 0.6747233658614036 1105 26-27 0.5979419245990769 1105 28-29 0.5468724873900754 1105 30-31 0.5934367359468737 1105 32-33 0.6050509314876038 1105 34-35 0.5315270984997866 1105 36-37 0.5400553179919214 1105 38-39 0.5685470217846742 1105 40-41 0.6405512791618762 1105 42-43 0.6283223730577987 1105 44-45 0.6429839256458649 1105 46-47 0.4608112883646882 1105 48-49 0.4667610399802271 1105 50-51 0.4560977936813515 1105 52-53 0.4291201383157457 1105 54-55 0.45425353123717827 1105 56-57 0.4344529293602122 1105 58-59 0.5508365432499644 1105 60-61 0.5708909701355509 1105 62-63 0.653379391517916 1105 64-65 0.710202066102184 1105 66-67 0.6617592336109368 1105 68-69 0.5183788647249017 1105 70-71 0.5306755788217501 1105 72-73 0.47744613091121124 1105 74-75 0.4866620341908501 1105 76-77 0.5028772040471523 1105 78-79 0.6063867159527305 1105 80-81 0.6161424311272015 1105 82-83 0.6981697065535926 1105 84-85 0.6865946090236008 1105 86-87 0.6170823012258104 1105 88-89 0.7733370311355579 1105 90-91 0.8687890440127539 1105 92-93 0.8776853404000953 1105 94-95 0.8196310590341369 1105 96-97 0.918544467415277 1105 98-99 1.0286329544941495 1105 100-101 1.0408703756861506 1106 1 0.08524246911848721 1106 2 0.3139429238546114 1106 3 0.07862205229170272 1106 4 0.038686608455208216 1106 5 0.05701593796880644 1106 6 0.12305236337065395 1106 7 0.17504686321962737 1106 8 0.13395391469025952 1106 9 0.0022643277604501577 1106 10-11 -0.033475403909910995 1106 12-13 -0.12565370026754152 1106 14-15 -0.17012631491964214 1106 16-17 -0.16007590793153526 1106 18-19 -0.10020688302390823 1106 20-21 -0.2820430519166166 1106 22-23 -0.29191721765479883 1106 24-25 0.07933653298954368 1106 26-27 -0.10488811287655864 1106 28-29 -0.15914341674398003 1106 30-31 -0.050173811976669924 1106 32-33 -0.06549081948175939 1106 34-35 -0.010239925116032111 1106 36-37 0.05588741353832205 1106 38-39 0.04064790219861436 1106 40-41 0.05916013380357299 1106 42-43 -0.004851475299318508 1106 44-45 -0.06618681310753516 1106 46-47 -0.07482217223775933 1106 48-49 0.02086418191138506 1106 50-51 -0.032530789904441804 1106 52-53 -0.08808859257392498 1106 54-55 -0.09322025573514026 1106 56-57 0.06067706688327945 1106 58-59 0.23291428878825116 1106 60-61 0.32868936043291086 1106 62-63 0.12757479843369168 1106 64-65 0.2108506351576125 1106 66-67 0.2285775153176317 1106 68-69 0.2367907597401313 1106 70-71 0.13321960534299393 1106 72-73 0.13885140384003591 1106 74-75 0.07580395936404472 1106 76-77 0.2030213575596207 1106 78-79 0.21333982598169143 1106 80-81 0.1712765578567641 1106 82-83 0.10985894839362231 1106 84-85 0.20467388186984437 1106 86-87 0.2523850995996888 1106 88-89 0.20630407876187107 1106 90-91 0.04724721677903787 1106 92-93 0.06791413569033367 1106 94-95 0.07834992450694145 1106 96-97 0.083796690765233 1106 98-99 0.06714781241440093 1106 100-101 0.14164746153642582 1107 1 -0.004051485596452409 1107 2 0.037307905722602897 1107 3 -0.04072488382042749 1107 4 -0.15346921411146752 1107 5 -0.19535346679157328 1107 6 -0.08793142291057165 1107 7 -0.14662637548948254 1107 8 -0.19400335551975445 1107 9 -0.2587061490697593 1107 10-11 -0.3958431012097279 1107 12-13 -0.4548320700970905 1107 14-15 -0.45548780427162683 1107 16-17 -0.5550488172944767 1107 18-19 -0.4337093460726251 1107 20-21 -0.49580660959423994 1107 22-23 -0.5237989728398418 1107 24-25 -0.43221379171338725 1107 26-27 -0.5296713527462202 1107 28-29 -0.665447590744563 1107 30-31 -0.666638370578319 1107 32-33 -0.40749565201540605 1107 34-35 -0.3485374450376426 1107 36-37 -0.2568159251141111 1107 38-39 -0.25021452247339226 1107 40-41 -0.3853323120257812 1107 42-43 -0.32101770689692444 1107 44-45 -0.3789622445101344 1107 46-47 -0.47955793554711335 1107 48-49 -0.5093678000510309 1107 50-51 -0.5690198985179364 1107 52-53 -0.4049344548180116 1107 54-55 -0.35258143974848366 1107 56-57 -0.4009234520405016 1107 58-59 -0.4151453620628587 1107 60-61 -0.5728801610656618 1107 62-63 -0.49230920245083354 1107 64-65 -0.4561253748624594 1107 66-67 -0.29970310219778895 1107 68-69 -0.3036407615666761 1107 70-71 -0.3883625652984506 1107 72-73 -0.5468648597424632 1107 74-75 -0.43138239580496673 1107 76-77 -0.36572847683112997 1107 78-79 -0.5838195747171255 1107 80-81 -0.43857364368202667 1107 82-83 -0.3954926593592063 1107 84-85 -0.2464492224125152 1107 86-87 -0.2174533370267433 1107 88-89 -0.28328889496129506 1107 90-91 -0.34629676502341056 1107 92-93 -0.26746577311554987 1107 94-95 -0.2669746844905845 1107 96-97 -0.29752039293764554 1107 98-99 -0.3555524310896061 1107 100-101 -0.5326225631319872 1108 1 -1.4069513147585404 1108 2 -5.4990486102896945 1108 3 -4.4867707725074375 1108 4 -3.1456743844767203 1108 5 -2.3410586642485143 1108 6 -1.2857582059949664 1108 7 -3.3172733089939577 1108 8 -3.969752431809443 1108 9 -2.014232825626763 1108 10-11 -4.178080404586844 1108 12-13 -3.4141561394617455 1108 14-15 -4.898190244591689 1108 16-17 -3.3145775418034518 1108 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0.7588409015769564 2305 100-101 0.7460715350166502 2306 1 -0.10008522076255133 2306 2 0.26458119100854205 2306 3 -0.0818852758783315 2306 4 -0.14358986216794278 2306 5 -0.073435682475818 2306 6 -0.30614880647735987 2306 7 -0.13875791782522384 2306 8 0.0015530793195779324 2306 9 -0.09050102789364445 2306 10-11 -0.10552527787575627 2306 12-13 -0.02813820115262189 2306 14-15 -0.2805761217477354 2306 16-17 -0.36662092467415164 2306 18-19 -0.09006709940572222 2306 20-21 -0.08085032090441047 2306 22-23 0.00169702747658107 2306 24-25 0.10258616431759293 2306 26-27 0.0703407104115783 2306 28-29 -0.13628295775401966 2306 30-31 -0.018013016381445368 2306 32-33 -0.09923515159375285 2306 34-35 0.01634832830837496 2306 36-37 -0.0025969433491397353 2306 38-39 -0.23979648431802758 2306 40-41 -0.2181350352301692 2306 42-43 0.026165461954860803 2306 44-45 -0.0779313914520472 2306 46-47 -0.15129037104081533 2306 48-49 -0.06672749088254903 2306 50-51 -0.104994780574593 2306 52-53 -0.5330812801726807 2306 54-55 -0.3923759887120255 2306 56-57 -0.1447949309373513 2306 58-59 -1.4689402063794432E-4 2306 60-61 0.016592519230009373 2306 62-63 0.010766969265404214 2306 64-65 0.021800589467666498 2306 66-67 0.07421533977581163 2306 68-69 -0.06020112446043413 2306 70-71 -0.02690783589696366 2306 72-73 -0.18153241998771108 2306 74-75 -0.13378609391524066 2306 76-77 -0.12156799140976204 2306 78-79 -0.16150149630060184 2306 80-81 -0.12577504139513707 2306 82-83 -0.2000168866389309 2306 84-85 -0.32525160430621014 2306 86-87 -0.3826569837063296 2306 88-89 -0.45590515891748495 2306 90-91 -0.4329478343948914 2306 92-93 -0.46985006969068266 2306 94-95 -0.8839672064971218 2306 96-97 -0.7983787199949397 2306 98-99 -0.551072834794315 2306 100-101 -0.2727940659339616 2307 1 -0.22689559564100747 2307 2 0.013798500727052243 2307 3 -0.07386406361110076 2307 4 -0.24582767593103227 2307 5 -0.2641189715866119 2307 6 -0.23744984712653405 2307 7 -0.2715751180998893 2307 8 -0.27342110869715697 2307 9 -0.4866230556337996 2307 10-11 -0.45809128379558217 2307 12-13 -0.46792491628982447 2307 14-15 -0.6952779972463858 2307 16-17 -1.0578445138478259 2307 18-19 -1.2123809710120028 2307 20-21 -1.0602870384713228 2307 22-23 -0.7346396688780743 2307 24-25 -0.6925142557814823 2307 26-27 -1.481690118586421 2307 28-29 -0.9361046015297063 2307 30-31 -0.6759891150782593 2307 32-33 -0.9200959526835106 2307 34-35 -0.6781946377721724 2307 36-37 -0.9334443890878745 2307 38-39 -0.8731398431933428 2307 40-41 -0.7354528695318621 2307 42-43 -0.6654677985436876 2307 44-45 -1.1575733484592519 2307 46-47 -1.0046424765340234 2307 48-49 -0.846264811333107 2307 50-51 -0.8159952836964379 2307 52-53 -0.967453142730335 2307 54-55 -0.9025569526665933 2307 56-57 -0.7563140586208519 2307 58-59 -0.8684270074419729 2307 60-61 -1.0556162289734417 2307 62-63 -0.9498611478340919 2307 64-65 -0.9157251859251545 2307 66-67 -1.149010092387492 2307 68-69 -1.1485444613233255 2307 70-71 -1.217491446413426 2307 72-73 -1.1297990564114855 2307 74-75 -1.0869254709530267 2307 76-77 -1.432061435902881 2307 78-79 -1.1409245623306319 2307 80-81 -1.1845434905925991 2307 82-83 -1.072864493924392 2307 84-85 -0.8943798299426433 2307 86-87 -0.9251832447342245 2307 88-89 -0.8077092481428103 2307 90-91 -0.8785015968465224 2307 92-93 -0.934205199368467 2307 94-95 -1.26863380478499 2307 96-97 -1.2102104698574259 2307 98-99 -1.4251117188025866 2307 100-101 -1.2732766047574948 2308 1 -1.2185172889874956 2308 2 -1.2516747999193427 2308 3 -3.5685578897460637 2308 4 -3.325989632782978 2308 5 -2.23794452569215 2308 6 -1.3055718291134681 2308 7 -2.0763307700655744 2308 8 -1.911808290818776 2308 9 -2.5354697476109465 2308 10-11 -2.680590303059752 2308 12-13 -2.924651893279666 2308 14-15 -2.4963740718167813 2308 16-17 -2.418702391106649 2308 18-19 -3.269787550094904 2308 20-21 -2.9446815385848097 2308 22-23 -2.697393718644605 2308 24-25 -4.001542879157498 2308 26-27 -4.302195130812656 2308 28-29 -2.931682539614215 2308 30-31 -2.8744302966649258 2308 32-33 -2.6123113649541736 2308 34-35 -3.843860566775305 2308 36-37 -3.21009087429659 2308 38-39 -2.691226480395791 2308 40-41 -3.9785801453303193 2308 42-43 -3.2343706719958902 2308 44-45 -2.8355694696925866 2308 46-47 -2.2726442639727296 2308 48-49 -2.6738943125075068 2308 50-51 -2.369197375123292 2308 52-53 -3.6371995665850534 2308 54-55 -4.19008536792985 2308 56-57 -3.4656491717914975 2308 58-59 -4.099011445154634 2308 60-61 -3.9105337638757973 2308 62-63 -4.736198150757595 2308 64-65 -4.292041379527728 2308 66-67 -4.044765610558194 2308 68-69 -3.6432611275949753 2308 70-71 -2.7066680275882646 2308 72-73 -2.720628051398201 2308 74-75 -2.310718287864738 2308 76-77 -2.806215773696927 2308 78-79 -2.431180717949438 2308 80-81 -2.6939182239039248 2308 82-83 -2.48275693546724 2308 84-85 -2.7204664927495017 2308 86-87 -2.7868795371382014 2308 88-89 -3.4609804520781715 2308 90-91 -3.4517702756738835 2308 92-93 -3.358069206523812 2308 94-95 -3.145159698125717 2308 96-97 -3.105308168660681 2308 98-99 -3.4490665846800255 2308 100-101 -3.556976989250334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 591.0 11 4194.0 12 11138.0 13 18668.0 14 27027.0 15 33891.0 16 39661.0 17 43697.0 18 48419.0 19 55480.0 20 64184.0 21 73225.0 22 85055.0 23 98334.0 24 115425.0 25 135040.0 26 158750.0 27 185326.0 28 218340.0 29 258330.0 30 308611.0 31 374943.0 32 465543.0 33 598803.0 34 826267.0 35 1229167.0 36 1855794.0 37 2367207.0 38 1987740.0 39 354490.0 40 115.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.09660715329554 11.870064539613878 14.36787745975906 37.66545084733153 2 23.87425567449338 18.982743654768804 30.311968642882682 26.831032027855134 3 27.164907065666398 19.25442460424546 22.7896408421175 30.79102748797064 4 28.803865604578622 23.349953161246372 20.831155800424565 27.015025433750438 5 29.164577011781333 26.220837274616187 22.742192938638212 21.872392774964265 6 25.351767798213405 30.516481315662215 22.400978589534432 21.730772296589947 7 21.056032421258482 21.133111625101634 37.11123285541957 20.699623098220314 8 22.699663618150804 23.596789825113323 28.87082412224531 24.832722434490563 9 22.025006775463787 22.17163412229845 31.42728299916569 24.376076103072077 10-11 24.78634455093289 29.59183809032889 23.712570544534724 21.9092468142035 12-13 23.828890976145054 25.2183011255241 27.215066007631034 23.737741890699812 14-15 23.861057822605073 25.857498877398648 27.119541101823263 23.161902198173014 16-17 24.337557259311613 25.622794652963403 26.425180612608205 23.614467475116776 18-19 24.24113144931156 25.68085938122745 26.429830440697422 23.648178728763572 20-21 24.034093541603596 25.59692593171361 26.33026206601528 24.038718460667514 22-23 24.124045321630206 25.95175352850238 26.040183790467275 23.88401735940014 24-25 23.861425978178506 26.068909043112075 26.23503386182952 23.834631116879898 26-27 23.77966549862607 26.054548518467207 26.143763443319962 24.022022539586764 28-29 23.966239424962403 26.102191928961254 25.843661487201015 24.08790715887533 30-31 23.970735274220445 25.947587714770577 25.97088424238741 24.11079276862157 32-33 24.062408521344565 25.809781357113447 26.031154773197702 24.096655348344285 34-35 24.310856832930824 25.94531426736875 25.6702469494638 24.073581950236626 36-37 24.364390058780078 25.783954043406908 25.833653251312548 24.018002646500467 38-39 24.07509522641526 25.88537275430979 25.868434018892668 24.171098000382287 40-41 24.173750638677387 26.241058049552024 25.509934098167193 24.0752572136034 42-43 24.064890509833724 26.16214149825432 25.8949466000457 23.878021391866255 44-45 24.222403882002148 25.976545406687716 25.973311235237606 23.82773947607253 46-47 24.348342410337725 26.011476628317272 25.97854142771209 23.661639533632908 48-49 24.143200112098896 26.041810812353717 26.121766387976354 23.693222687571037 50-51 23.827698912142058 26.06295480941987 26.13885264771608 23.970493630721997 52-53 24.118855909755645 25.97964499981566 25.793591416649424 24.10790767377927 54-55 24.067657015418753 25.91938638054765 25.789002378027824 24.223954226005777 56-57 23.87206583125137 25.964275668365104 26.02620420932229 24.137454291061236 58-59 24.190155235711604 25.99163563541155 25.829602746239093 23.98860638263775 60-61 24.067500002594876 25.83646258136235 26.075432020983207 24.02060539505956 62-63 23.911341758723008 26.02129437275665 26.117538553722447 23.949825314797895 64-65 24.43296693378408 25.93001651394818 25.942492169294766 23.694524382972975 66-67 24.319119860611437 26.08211878716541 25.65471655312229 23.944044799100855 68-69 24.351095731989222 25.735195943755677 25.955082992788782 23.958625331466315 70-71 24.275311837399496 25.734847206648986 25.868027416714934 24.121813539236577 72-73 24.204925064699037 25.64376869895153 25.841921953299785 24.30938428304965 74-75 24.26624052099331 25.860023069789932 25.807866114178523 24.065870295038234 76-77 24.26561362452771 25.64543765510498 26.0950635764352 23.993885143932108 78-79 24.108664431868494 25.764781236995 26.055952101408863 24.07060222972764 80-81 24.164998817615142 25.88228827339926 25.9317383648016 24.020974544183996 82-83 24.107961247984484 25.733562581024326 25.724798481764072 24.433677689227114 84-85 23.942451390707824 25.734765364436374 25.771731505419105 24.551051739436698 86-87 24.009519023204074 25.902649637213866 25.64416464738242 24.443666692199635 88-89 24.08758748319419 25.925938534586752 25.71265258078744 24.27382140143162 90-91 23.99232327758035 25.75823347867686 25.879822684933128 24.36962055880966 92-93 24.096305598045035 25.86401094942733 25.736776424748886 24.30290702777875 94-95 24.107187527935 25.982065382048663 25.644036567431854 24.266710522584475 96-97 24.300589964525074 25.79835189890991 25.572149841623272 24.32890829494174 98-99 24.225231703641867 25.834856947825312 25.44528021878771 24.494631129745116 100-101 24.198996694952154 25.872420038334702 25.551829400943056 24.376753865770088 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1752.0 1 1312.0 2 900.0 3 1054.0 4 1160.0 5 1043.5 6 892.0 7 815.5 8 745.0 9 641.5 10 588.5 11 607.5 12 658.0 13 734.5 14 860.0 15 1060.0 16 1358.0 17 1794.5 18 2569.5 19 3716.0 20 5369.5 21 7876.0 22 11399.5 23 16620.5 24 24034.5 25 33420.5 26 45441.5 27 60342.0 28 77894.5 29 97515.5 30 119908.5 31 143681.5 32 164970.0 33 187105.0 34 212026.5 35 236485.5 36 259334.5 37 283033.0 38 307656.0 39 326753.5 40 342610.0 41 357373.0 42 367936.0 43 376549.5 44 380522.0 45 382701.0 46 381919.5 47 372169.5 48 361709.0 49 353402.0 50 348592.0 51 357923.5 52 357645.0 53 342363.0 54 334527.5 55 323140.5 56 302828.0 57 294053.0 58 292136.0 59 291569.5 60 289153.0 61 283989.5 62 281217.0 63 274901.5 64 257538.0 65 219563.0 66 171271.0 67 136146.5 68 119646.5 69 100241.5 70 75101.5 71 55783.5 72 45788.0 73 38074.5 74 30083.5 75 24423.0 76 20292.0 77 16709.5 78 13073.5 79 9106.5 80 5929.0 81 3698.5 82 2467.5 83 1597.0 84 679.5 85 344.5 86 233.5 87 175.0 88 105.0 89 62.0 90 48.5 91 35.0 92 21.5 93 11.5 94 7.0 95 8.0 96 5.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.660652889004618E-5 3 4.981958667013854E-5 4 8.303264445023089E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.660652889004618E-5 22-23 5.604703500390586E-4 24-25 8.469329733923552E-4 26-27 5.604703500390586E-4 28-29 0.0 30-31 0.06084217022090669 32-33 3.7364690002603906E-5 34-35 3.3628221002343514E-4 36-37 3.7364690002603906E-5 38-39 4.483762800312469E-4 40-41 1.0379080556278862E-4 42-43 0.0 44-45 0.0015444071867742948 46-47 9.175107211750514E-4 48-49 0.004305242614744471 50-51 0.002437008114614277 52-53 0.003487371066909698 54-55 0.011080706401883314 56-57 0.00451282422587005 58-59 0.001843324706795126 60-61 0.004097661003618894 62-63 0.005397121889265008 64-65 4.151632222511545E-6 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.963917334027708E-5 80-81 0.0 82-83 4.1516322225115446E-5 84-85 2.0758161112557723E-5 86-87 0.0016897143145621987 88-89 0.0 90-91 2.9061425557580815E-4 92-93 0.015884144883329173 94-95 0.008232686697240393 96-97 1.6606528890046178E-4 98-99 4.151632222511545E-6 100-101 4.981958667013855E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2043456E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.49264638656794 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00284620485748 56.52064194551407 2 8.987830862243017 11.952493186108141 3 2.6311723088592744 5.248608297453278 4 1.128928928964114 3.0026188827671088 5 0.5900330978377869 1.9616431065449604 6 0.3655510883991333 1.4583875554289167 7 0.24740614092291519 1.1515482329576958 8 0.17014517541835533 0.905072238677862 9 0.12959338899569292 0.77553066496748 >10 0.6850181284620802 8.058458481389218 >50 0.03841343355089353 1.732424158248718 >100 0.01866027329591186 2.400800634172521 >500 0.0020973522770491673 1.000535770497745 >1k 0.0020782655405608653 2.7174904810605307 >5k 1.8779197977558828E-4 0.8248271165593183 >10k+ 3.7558395955117654E-5 0.2889192476527417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT 13098 0.10875615770091242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.30326444502309E-6 0.0 2 8.30326444502309E-6 4.1516322225115446E-5 0.0 7.472938000520781E-5 0.0 3 8.30326444502309E-6 7.472938000520781E-5 0.0 1.0794243778530016E-4 0.0 4 1.660652889004618E-5 7.472938000520781E-5 0.0 1.1624570223032326E-4 0.0 5 4.1516322225115446E-5 9.133590889525399E-5 0.0 1.1624570223032326E-4 0.0 6 4.1516322225115446E-5 9.963917334027708E-5 0.0 1.2454896667534634E-4 0.0 7 4.981958667013854E-5 1.2454896667534634E-4 0.0 1.2454896667534634E-4 0.0 8 6.642611556018472E-5 1.6606528890046178E-4 0.0 1.4115549556539253E-4 0.0 9 8.303264445023089E-5 2.1588487557060032E-4 0.0 1.4115549556539253E-4 0.0 10-11 1.1624570223032324E-4 2.2833977223813497E-4 0.0 1.4530712778790407E-4 0.0 12-13 1.9512671445804262E-4 2.490979333506927E-4 8.30326444502309E-6 1.5776202445543872E-4 4.151632222511545E-6 14-15 3.8610179669357366E-4 2.6570446224073887E-4 1.2454896667534635E-5 1.7436855334548488E-4 8.30326444502309E-6 16-17 4.4837628003124684E-4 2.9061425557580815E-4 1.660652889004618E-5 1.7852018556799643E-4 8.30326444502309E-6 18-19 4.815893378113392E-4 3.0722078446585434E-4 2.490979333506927E-5 2.0758161112557723E-4 8.30326444502309E-6 20-21 6.102899367091972E-4 3.362822100234351E-4 2.490979333506927E-5 2.1588487557060032E-4 8.30326444502309E-6 22-23 0.001066969481185467 3.611920033585044E-4 2.9061425557580814E-5 2.2003650779311187E-4 1.660652889004618E-5 24-25 0.0016274398312245255 4.1101159002864294E-4 3.321305778009236E-5 2.2418814001562342E-4 1.660652889004618E-5 26-27 0.002698560944632504 4.359213833637122E-4 3.321305778009236E-5 2.2418814001562342E-4 2.0758161112557723E-5 28-29 0.004512824225870049 4.5667954447626993E-4 3.321305778009236E-5 2.2833977223813497E-4 2.490979333506927E-5 30-31 0.006816980109363957 4.5667954447626993E-4 3.321305778009236E-5 2.3249140446064651E-4 2.490979333506927E-5 32-33 0.010225470164045935 4.8574097003385074E-4 4.1516322225115446E-5 2.3249140446064651E-4 2.490979333506927E-5 34-35 0.014443528502117664 5.189540278139431E-4 4.1516322225115446E-5 2.3664303668315806E-4 2.490979333506927E-5 36-37 0.01935906105357133 5.646219822615701E-4 4.1516322225115446E-5 2.4494630112818116E-4 2.490979333506927E-5 38-39 0.024478023583928067 5.853801433741278E-4 4.1516322225115446E-5 2.615528300182273E-4 2.490979333506927E-5 40-41 0.029223339214258764 6.01986672264174E-4 4.1516322225115446E-5 2.6570446224073887E-4 2.490979333506927E-5 42-43 0.03320890614786985 6.268964655992432E-4 4.1516322225115446E-5 2.6985609446325044E-4 2.490979333506927E-5 44-45 0.03708653064369563 6.808676844918933E-4 4.1516322225115446E-5 2.8646262335329663E-4 2.490979333506927E-5 46-47 0.041267224291764756 7.555970644971012E-4 4.1516322225115446E-5 3.0722078446585434E-4 2.490979333506927E-5 48-49 0.04654810047879944 7.84658490054682E-4 4.1516322225115446E-5 3.1552404891087743E-4 2.490979333506927E-5 50-51 0.05194937400028696 8.137199156122627E-4 4.566795444762699E-5 3.4043384224594666E-4 2.490979333506927E-5 52-53 0.059127546113009424 8.469329733923551E-4 4.981958667013854E-5 3.4458547446845824E-4 2.490979333506927E-5 54-55 0.07097630447605738 8.593878700598898E-4 4.981958667013854E-5 3.4873710669096976E-4 2.490979333506927E-5 56-57 0.0787190985710414 8.926009278399822E-4 5.812285111516163E-5 3.7364690002603904E-4 2.490979333506927E-5 58-59 0.08582669293598116 9.050558245075168E-4 5.812285111516163E-5 3.985566933611083E-4 2.490979333506927E-5 60-61 0.09246515285977713 9.299656178425861E-4 5.812285111516163E-5 4.4837628003124684E-4 2.9061425557580814E-5 62-63 0.1004362867269993 9.714819400677015E-4 5.812285111516163E-5 4.898926022563623E-4 3.73646900026039E-5 64-65 0.10799640900419281 9.880884689577477E-4 6.227448333767317E-5 5.064991311464085E-4 4.1516322225115446E-5 66-67 0.11626230875921331 9.922401011802592E-4 6.642611556018472E-5 5.272572922589662E-4 4.1516322225115446E-5 68-69 0.12483958093092215 0.0010088466300703055 6.642611556018472E-5 5.687736144840816E-4 4.1516322225115446E-5 70-71 0.13378634837043452 0.0010462113200729092 6.642611556018472E-5 6.061383044866856E-4 4.1516322225115446E-5 72-73 0.15104883515163753 0.0011001825389655594 6.642611556018472E-5 6.393513622667779E-4 4.1516322225115446E-5 74-75 0.16173513649238225 0.0011084858034105824 6.642611556018472E-5 6.725644200468702E-4 4.1516322225115446E-5 76-77 0.17393678359434367 0.0011292439645231402 6.642611556018472E-5 6.808676844918933E-4 4.1516322225115446E-5 78-79 0.19002851008879845 0.0011333955967456517 7.057774778269626E-5 7.01625845604451E-4 4.1516322225115446E-5 80-81 0.20631121166548871 0.0011375472289681633 7.888101222771934E-5 7.182323744944973E-4 4.1516322225115446E-5 82-83 0.2255125106946046 0.0011416988611906748 8.303264445023089E-5 7.389905356070549E-4 4.1516322225115446E-5 84-85 0.24709684661944212 0.0011707602867482556 8.303264445023089E-5 7.680519611646358E-4 4.981958667013854E-5 86-87 0.2745640454035785 0.0011790635511932787 8.303264445023089E-5 8.510846056148666E-4 4.981958667013854E-5 88-89 0.30956645667157334 0.0011915184478608135 9.133590889525399E-5 8.926009278399822E-4 5.397121889265009E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGAT 4345 0.0 31.917194 1 GGCGCGT 7650 0.0 31.041319 5 GGCGATC 4935 0.0 28.582546 2 GCGATCT 5110 0.0 26.860157 3 TGGCGCG 8745 0.0 26.828644 4 GCGCGTG 9320 0.0 25.530153 6 CGCGTGC 9270 0.0 25.462923 7 CGGTGGC 9930 0.0 24.53577 1 CGATCTG 5730 0.0 24.036707 4 GTGGCGC 10200 0.0 23.187864 3 GCGTGCC 10120 0.0 23.089588 8 GGTGGCG 10460 0.0 22.56609 2 CGTGCCT 10730 0.0 21.909733 9 GCGTTCG 4195 0.0 17.94723 44-45 GTGCGTT 4235 0.0 17.496077 42-43 AAGTGCG 4380 0.0 17.132896 40-41 CTCGCTA 4070 0.0 16.453426 1 AGTGTCG 4730 0.0 15.867309 52-53 GCGACGC 2095 0.0 15.642158 1 ACCGCGT 2630 0.0 15.34952 8 >>END_MODULE