FastQCFastQC Report
Thu 26 May 2016
SRR1567913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12043456
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT134940.11204425042114158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT44500.031.9403971
GGCGATC50500.028.1244662
GCGATCT50050.027.5215633
CGATCTG53550.025.1905754
GGCGCGT62950.023.0135355
TGGCGCG76800.018.8014584
GCGCGTG81350.017.8666486
CGGTGGC86850.017.6791691
CGCGTGC82550.017.6644657
GCGACGC21150.017.3065091
CGACGCT21300.016.9487782
GCGTGCC91150.016.0499348
CTCGCTA39500.015.8856861
GTGCGTT40600.015.8538242-43
AAGTGCG41450.015.70068240-41
GTGGCGC93550.015.5874033
GCGTTCG43200.015.339498544-45
GGTGGCG95550.015.3117372
CGTGCCT95800.015.2708929
CCGCGCT33200.015.1660412
Produced by FastQC (version 0.11.5)