##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567913_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12043456 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811805431929173 31.0 31.0 34.0 30.0 34.0 2 32.0365851795365 33.0 31.0 34.0 30.0 34.0 3 32.259201096429464 33.0 31.0 34.0 30.0 34.0 4 36.04604982157945 37.0 35.0 37.0 35.0 37.0 5 35.98885212018876 37.0 35.0 37.0 35.0 37.0 6 35.97512615979998 37.0 35.0 37.0 35.0 37.0 7 35.961952615594726 37.0 35.0 37.0 35.0 37.0 8 35.97019916874359 37.0 35.0 37.0 35.0 37.0 9 37.72634981188124 39.0 37.0 39.0 35.0 39.0 10-11 37.710902667805655 39.0 37.0 39.0 35.0 39.0 12-13 37.646071733894324 39.0 37.0 39.0 35.0 39.0 14-15 38.939238579025826 40.0 38.0 41.0 36.0 41.0 16-17 38.87571586594413 40.0 38.0 41.0 35.0 41.0 18-19 38.87411524565706 40.0 38.0 41.0 35.0 41.0 20-21 38.85307826092444 40.0 38.0 41.0 35.0 41.0 22-23 38.737345077691984 40.0 38.0 41.0 35.0 41.0 24-25 38.649763821946124 40.0 38.0 41.0 34.0 41.0 26-27 38.474389867825316 40.0 38.0 41.0 34.0 41.0 28-29 38.325730712180956 40.0 38.0 41.0 34.0 41.0 30-31 38.21293368780523 40.0 38.0 41.0 34.0 41.0 32-33 38.05012892478704 40.0 37.5 41.0 33.0 41.0 34-35 37.913678557052066 40.0 37.0 41.0 33.0 41.0 36-37 37.89739232658798 40.0 37.0 41.0 33.0 41.0 38-39 38.00741141081099 40.0 37.0 41.0 33.0 41.0 40-41 37.97341315482865 40.0 37.0 41.0 33.0 41.0 42-43 37.82275855867286 40.0 37.0 41.0 33.0 41.0 44-45 37.664707539098416 39.5 37.0 41.0 33.0 41.0 46-47 37.543573663572985 39.0 36.0 41.0 32.5 41.0 48-49 37.35908056624278 39.0 36.0 41.0 32.0 41.0 50-51 37.15528719497128 39.0 35.0 40.0 31.5 41.0 52-53 36.926042823588176 39.0 35.0 40.0 31.0 41.0 54-55 36.65979271232443 38.0 35.0 40.0 31.0 41.0 56-57 36.39278505272905 38.0 35.0 40.0 30.5 41.0 58-59 36.09039132953198 38.0 34.5 40.0 30.0 41.0 60-61 35.801912964185696 37.0 34.0 40.0 30.0 41.0 62-63 35.56705255534624 37.0 34.0 40.0 29.0 41.0 64-65 35.32805350889313 36.5 34.0 40.0 29.0 41.0 66-67 35.05254970001966 36.0 34.0 39.0 29.0 41.0 68-69 34.71002912286971 35.5 34.0 39.0 29.0 41.0 70-71 34.42389306690704 35.0 33.0 39.0 28.5 40.0 72-73 34.089901935125596 35.0 33.0 38.0 28.0 40.0 74-75 33.74625140823365 35.0 33.0 37.0 28.0 39.5 76-77 32.96198852721345 35.0 32.0 36.0 26.5 39.0 78-79 33.09862804331249 35.0 33.0 36.0 27.0 39.0 80-81 32.94326711535294 35.0 33.0 36.0 27.0 38.5 82-83 32.63872172572391 35.0 33.0 35.5 27.0 37.0 84-85 32.35201203873705 35.0 32.0 35.0 26.0 37.0 86-87 32.10938579424378 35.0 32.0 35.0 26.0 36.0 88-89 31.915838775846403 35.0 32.0 35.0 26.0 36.0 90-91 31.729805547510615 34.0 32.0 35.0 25.5 36.0 92-93 31.477061194062568 34.0 32.0 35.0 25.0 35.5 94-95 31.196223243560652 34.0 32.0 35.0 24.0 35.0 96-97 30.986032165517937 34.0 32.0 35.0 24.0 35.0 98-99 30.71127797535857 34.0 31.0 35.0 23.0 35.0 100-101 30.23742649119987 34.0 31.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39749618759262617 1101 2 0.49489305302057573 1101 3 0.42502652543298325 1101 4 0.25213337898238564 1101 5 0.20327544365446215 1101 6 0.23062440899977332 1101 7 0.262688656904956 1101 8 0.2837732259040422 1101 9 0.2765560532877629 1101 10-11 0.24109533326027588 1101 12-13 0.31495940554806623 1101 14-15 0.39713919944730236 1101 16-17 0.44299389672536194 1101 18-19 0.4186351911570867 1101 20-21 0.36896779808439106 1101 22-23 0.3941493703891581 1101 24-25 0.36913435538546224 1101 26-27 0.4021501779030672 1101 28-29 0.40379968309896697 1101 30-31 0.24602672684689963 1101 32-33 0.30646127013834956 1101 34-35 0.21510803498071596 1101 36-37 0.28293057850181214 1101 38-39 0.37405027817593606 1101 40-41 0.3513147214688104 1101 42-43 0.3378483253689737 1101 44-45 0.3732408818983757 1101 46-47 0.2994969778538561 1101 48-49 0.3233223212522347 1101 50-51 0.3099933717130199 1101 52-53 0.3270771990892527 1101 54-55 0.23054502066981541 1101 56-57 0.21814316229204422 1101 58-59 0.1639247236195729 1101 60-61 0.17372738461859427 1101 62-63 0.22412074445730923 1101 64-65 0.18867892110371542 1101 66-67 0.16023817853292144 1101 68-69 0.1334801094943927 1101 70-71 0.07899454500036285 1101 72-73 0.028443727626196846 1101 74-75 0.006251853253480988 1101 76-77 -0.09420802736423184 1101 78-79 -0.03986238657283536 1101 80-81 -0.09584661276617368 1101 82-83 -0.08281801440192993 1101 84-85 -0.15760977189570724 1101 86-87 -0.19528756325257746 1101 88-89 -0.20490672282211975 1101 90-91 -0.18517406213988608 1101 92-93 -0.2026214618959763 1101 94-95 -0.2927930095639262 1101 96-97 -0.45271178217247865 1101 98-99 -0.5565618054993742 1101 100-101 -0.569966510739917 1102 1 0.2896456396640623 1102 2 0.3588148430283269 1102 3 0.35137334830033495 1102 4 0.22484751215910137 1102 5 0.20860312761070077 1102 6 0.161216148964904 1102 7 0.1750750864603674 1102 8 0.17361673658535182 1102 9 0.22075380652590582 1102 10-11 0.1798939923380658 1102 12-13 0.18352537923689738 1102 14-15 0.22853485286864839 1102 16-17 0.26502443791743246 1102 18-19 0.25074952935604244 1102 20-21 0.2614225861538628 1102 22-23 0.24428533813713926 1102 24-25 0.28120260322762647 1102 26-27 0.2714930869473662 1102 28-29 0.2606754445557229 1102 30-31 0.2645904004984274 1102 32-33 0.2659306761243627 1102 34-35 0.2362469984896407 1102 36-37 0.2097316662656965 1102 38-39 0.2694637163380449 1102 40-41 0.31638814622454703 1102 42-43 0.38219359673090025 1102 44-45 0.35295147214160494 1102 46-47 0.30455223172172197 1102 48-49 0.3114765113366431 1102 50-51 0.2840289855983613 1102 52-53 0.3497292622847752 1102 54-55 0.3479703688549023 1102 56-57 0.34909611442437694 1102 58-59 0.32652983557544957 1102 60-61 0.2665790119358249 1102 62-63 0.29216894009881145 1102 64-65 0.35206485123939046 1102 66-67 0.3924000240686283 1102 68-69 0.41419924761798654 1102 70-71 0.33001485678905595 1102 72-73 0.2437073017268503 1102 74-75 0.2809639025900097 1102 76-77 0.26433615405285593 1102 78-79 0.34827817940643513 1102 80-81 0.21503767387627448 1102 82-83 0.2971718590462018 1102 84-85 0.16746139032778729 1102 86-87 0.21609807585834773 1102 88-89 0.2827370296236289 1102 90-91 0.31970422619141914 1102 92-93 0.2846337142807194 1102 94-95 0.18227501765533916 1102 96-97 0.08111031342312813 1102 98-99 0.09979067534112573 1102 100-101 0.2089369356832549 1103 1 0.23282782965582172 1103 2 0.31782550543573507 1103 3 0.2980445844040531 1103 4 0.17925469012900663 1103 5 0.14125342007364594 1103 6 0.1020392278413027 1103 7 0.10071974522238492 1103 8 0.14682565466249287 1103 9 0.14774324216615753 1103 10-11 0.14510526943452362 1103 12-13 0.17075186253364194 1103 14-15 0.19250431230840803 1103 16-17 0.21186695446665027 1103 18-19 0.203714461160601 1103 20-21 0.21168808599220057 1103 22-23 0.23057816536339715 1103 24-25 0.25421483843697956 1103 26-27 0.20903820677207108 1103 28-29 0.2251326543727572 1103 30-31 0.19951053296924925 1103 32-33 0.12609026796700817 1103 34-35 0.13276193671135417 1103 36-37 0.17406648175891348 1103 38-39 0.16370102949436927 1103 40-41 0.13556058543179006 1103 42-43 0.14670846003979676 1103 44-45 0.16750280036605858 1103 46-47 0.0655587515237599 1103 48-49 0.06243023596336883 1103 50-51 0.03518638862803414 1103 52-53 -0.0185370715094777 1103 54-55 -0.05487453719285895 1103 56-57 0.012773863720191514 1103 58-59 -0.027113323461342986 1103 60-61 -0.13665365056669287 1103 62-63 -0.12008139870726353 1103 64-65 -0.30800806757512333 1103 66-67 -0.2529400406465001 1103 68-69 -0.2593227527892594 1103 70-71 -0.28647057165809287 1103 72-73 -0.44864741376877504 1103 74-75 -0.4114178402750568 1103 76-77 -0.43497333521615644 1103 78-79 -0.40327522416011163 1103 80-81 -0.38196447307365844 1103 82-83 -0.26582297178578784 1103 84-85 -0.34535200643418307 1103 86-87 -0.37268414284999096 1103 88-89 -0.27416670979286195 1103 90-91 -0.22900285753154748 1103 92-93 -0.15459919315733472 1103 94-95 -0.1995116750741346 1103 96-97 -0.19290504664220975 1103 98-99 -0.1787452142329755 1103 100-101 -0.0388343437340275 1104 1 0.11007968599911067 1104 2 0.2039981771293995 1104 3 0.1687581288261697 1104 4 0.10386332462321235 1104 5 0.0433949170914687 1104 6 -0.04165844635802074 1104 7 -0.03983145097576113 1104 8 0.007284037736837945 1104 9 -0.08008908938409576 1104 10-11 0.04007120873347958 1104 12-13 0.07838587669427 1104 14-15 0.07107711675074313 1104 16-17 0.09586643698219177 1104 18-19 0.07172571956114382 1104 20-21 0.08306377833193324 1104 22-23 0.042593342196070694 1104 24-25 -0.08075714777133669 1104 26-27 0.006665398257126753 1104 28-29 0.09872960174911327 1104 30-31 0.011515953233086407 1104 32-33 0.06253495786850038 1104 34-35 -0.0027540247216037983 1104 36-37 -0.05692348742828557 1104 38-39 -0.33751992613580484 1104 40-41 -0.21391688988799729 1104 42-43 -0.09390216438651322 1104 44-45 -0.3364324770656495 1104 46-47 -0.18697387156679923 1104 48-49 -0.24463945680258803 1104 50-51 -0.09045004631132514 1104 52-53 -0.2713588801351179 1104 54-55 -0.11393546005505328 1104 56-57 -0.178333350436084 1104 58-59 -0.30741917097233795 1104 60-61 -0.19238129051119301 1104 62-63 0.04833724063620792 1104 64-65 -0.03845699530734237 1104 66-67 -0.17642407528558124 1104 68-69 -0.09939106666492847 1104 70-71 0.093953509273355 1104 72-73 0.03264701166803974 1104 74-75 -0.03496293752982638 1104 76-77 -0.032350882156940486 1104 78-79 0.1500179502045924 1104 80-81 0.10172641924410897 1104 82-83 0.11610879880687719 1104 84-85 0.11484018363240978 1104 86-87 0.12729243999407203 1104 88-89 0.27424403739190595 1104 90-91 0.13488166970268622 1104 92-93 0.27903075676346845 1104 94-95 0.23422201835622047 1104 96-97 0.17665368485661048 1104 98-99 0.2351522984782939 1104 100-101 0.3369120851616678 1105 1 0.06447458849622123 1105 2 0.11902779751532933 1105 3 0.13040542629655505 1105 4 0.040509606826795164 1105 5 0.044203055455412255 1105 6 -0.008083002772337977 1105 7 -0.008626716398211443 1105 8 -0.020652613655805396 1105 9 -0.10341401290870778 1105 10-11 -0.014250073923079754 1105 12-13 -0.04348062519350293 1105 14-15 -0.04950769477381556 1105 16-17 0.010202637700359674 1105 18-19 -0.03707622143872413 1105 20-21 0.008564420643516257 1105 22-23 0.00822498021489082 1105 24-25 0.034623775945419766 1105 26-27 0.054356859269312 1105 28-29 0.12094859350433751 1105 30-31 0.07032466280121952 1105 32-33 0.08891695869356653 1105 34-35 0.08924618465159284 1105 36-37 0.04384607780280447 1105 38-39 0.11349879887949754 1105 40-41 0.13318458367470498 1105 42-43 -0.0033395587951687844 1105 44-45 0.07023313757296279 1105 46-47 0.07608629751327811 1105 48-49 0.16732363676134554 1105 50-51 0.10359083938066505 1105 52-53 0.18970335154232743 1105 54-55 0.14705463476824576 1105 56-57 0.2306975515716303 1105 58-59 0.27513321294159 1105 60-61 0.2022851971646915 1105 62-63 0.3331620375907747 1105 64-65 0.2770895206926127 1105 66-67 0.32735937259352 1105 68-69 0.3687413923243881 1105 70-71 0.41275250870957336 1105 72-73 0.3222129759101975 1105 74-75 0.4028317941281401 1105 76-77 0.33591538261818954 1105 78-79 0.41402076768703466 1105 80-81 0.4180994542327241 1105 82-83 0.5058414666502458 1105 84-85 0.45448969693964614 1105 86-87 0.5354819710259093 1105 88-89 0.5800403830641407 1105 90-91 0.616900716837975 1105 92-93 0.6285037350291525 1105 94-95 0.6208923517086333 1105 96-97 0.5348733236098262 1105 98-99 0.5694643726077935 1105 100-101 0.7419073497449133 1106 1 -0.07676583779482016 1106 2 -0.02830876906708113 1106 3 -0.06237580929423814 1106 4 -0.06733884791351841 1106 5 -0.118466842888445 1106 6 -0.12897422452130058 1106 7 -0.2033653900267467 1106 8 -0.20113607573048853 1106 9 -0.22152373325040742 1106 10-11 -0.21779066032434002 1106 12-13 -0.2253120259399779 1106 14-15 -0.28624722561136906 1106 16-17 -0.3087577466057212 1106 18-19 -0.2754503804783184 1106 20-21 -0.3063807797592446 1106 22-23 -0.3186932828734257 1106 24-25 -0.23822959668046906 1106 26-27 -0.3125319508196327 1106 28-29 -0.2586707039637588 1106 30-31 -0.25466729182932824 1106 32-33 -0.29082641500261275 1106 34-35 -0.2479401606729752 1106 36-37 -0.3112778092380566 1106 38-39 -0.2849050720989652 1106 40-41 -0.30979288735403543 1106 42-43 -0.393927128441824 1106 44-45 -0.38208126505165296 1106 46-47 -0.328979126041709 1106 48-49 -0.3602582875551761 1106 50-51 -0.33800163305027553 1106 52-53 -0.3211898302874019 1106 54-55 -0.3017007960491185 1106 56-57 -0.444216649633141 1106 58-59 -0.39729553399642725 1106 60-61 -0.272541789147553 1106 62-63 -0.35884010659668064 1106 64-65 -0.4792644601814473 1106 66-67 -0.2340145651475467 1106 68-69 -0.25926093956261553 1106 70-71 -0.3549363633732696 1106 72-73 -0.3184389531834384 1106 74-75 -0.14453483329481287 1106 76-77 -0.26684384227329616 1106 78-79 -0.2193440439876042 1106 80-81 -0.18940814069428313 1106 82-83 -0.07668120922181032 1106 84-85 -0.2120915828490979 1106 86-87 -0.26365669796557256 1106 88-89 -0.1725674155535195 1106 90-91 -0.19605510053324338 1106 92-93 -0.18544895589676358 1106 94-95 -0.2932835485720702 1106 96-97 -0.3857417500428113 1106 98-99 -0.352472794888282 1106 100-101 -0.16191453266635136 1107 1 -0.25641372393015516 1107 2 -0.2805315480464934 1107 3 -0.31061359682230716 1107 4 -0.22085988169514081 1107 5 -0.2787441005066711 1107 6 -0.3102429681961212 1107 7 -0.3554629674964076 1107 8 -0.3592788828046949 1107 9 -0.4361451290813747 1107 10-11 -0.4398735979760815 1107 12-13 -0.5086862233957916 1107 14-15 -0.6951735263812324 1107 16-17 -0.7317378996309998 1107 18-19 -0.6564244818809044 1107 20-21 -0.5992299345503582 1107 22-23 -0.5934780472286008 1107 24-25 -0.6186260881198393 1107 26-27 -0.5853403920576596 1107 28-29 -0.6896741246090983 1107 30-31 -0.6490428055070936 1107 32-33 -0.5865316259140556 1107 34-35 -0.5890323794913215 1107 36-37 -0.6814997528556503 1107 38-39 -0.6967344342874995 1107 40-41 -0.6613688114026388 1107 42-43 -0.8002935314388608 1107 44-45 -0.6191524662806884 1107 46-47 -0.840117534129611 1107 48-49 -0.6364593017069069 1107 50-51 -0.5921030358760291 1107 52-53 -0.6011732827403264 1107 54-55 -0.601239844905038 1107 56-57 -0.4603156095132519 1107 58-59 -0.48424912946705234 1107 60-61 -0.39246772948125397 1107 62-63 -0.6177197274389314 1107 64-65 -0.485436041657465 1107 66-67 -0.2930813205610576 1107 68-69 -0.24239932035931844 1107 70-71 -0.22854243879376668 1107 72-73 -0.29367094292531704 1107 74-75 -0.1950433076234077 1107 76-77 -0.3499398146191197 1107 78-79 -0.2437536825395341 1107 80-81 -0.2418767504193724 1107 82-83 -0.23335341670537701 1107 84-85 -0.2909269348976977 1107 86-87 -0.17841346721606754 1107 88-89 -0.07429596003698791 1107 90-91 -0.1755665087493803 1107 92-93 -0.18354380434682227 1107 94-95 -0.20514744986486377 1107 96-97 -0.29176319039333265 1107 98-99 -0.29525476429816067 1107 100-101 -0.158735875911308 1108 1 -0.43799407784173994 1108 2 -0.4682860273276823 1108 3 -0.425207951884758 1108 4 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2305 9 0.03993903633230644 2305 10-11 0.09631759823983543 2305 12-13 0.06852248298125829 2305 14-15 0.07880916598644916 2305 16-17 0.09638326139703679 2305 18-19 0.08542449334818514 2305 20-21 0.030573637402291354 2305 22-23 -3.215712704047746E-4 2305 24-25 -0.01315022499831997 2305 26-27 0.04965266260822432 2305 28-29 0.11477523113974542 2305 30-31 -0.0776330890455128 2305 32-33 0.03314470841155526 2305 34-35 0.10009104716892381 2305 36-37 0.0972854885157659 2305 38-39 0.02829556659396104 2305 40-41 0.034896506278428774 2305 42-43 0.1429253918258908 2305 44-45 0.1887425417010391 2305 46-47 0.21998742729098808 2305 48-49 0.21533906868673114 2305 50-51 0.20554127609489825 2305 52-53 0.2654234194787719 2305 54-55 0.2722114302555809 2305 56-57 0.34239719434251725 2305 58-59 0.17715886689815363 2305 60-61 0.30562365276632164 2305 62-63 0.3471127704243244 2305 64-65 0.2925796105592724 2305 66-67 0.171116904116154 2305 68-69 0.30091783362016145 2305 70-71 0.33281846413734684 2305 72-73 0.4015149503781501 2305 74-75 0.35769454437271264 2305 76-77 0.4500604946665092 2305 78-79 0.42032074292283284 2305 80-81 0.403854304584776 2305 82-83 0.4194131776839356 2305 84-85 0.483971960772152 2305 86-87 0.5402639371915612 2305 88-89 0.47527459819621853 2305 90-91 0.49733510738062137 2305 92-93 0.49330103393459623 2305 94-95 0.4705361344356014 2305 96-97 0.5978526241790156 2305 98-99 0.6448326026956792 2305 100-101 0.7249776177621747 2306 1 -1.1833090860059698 2306 2 -0.7370254235654485 2306 3 -0.37290787905754996 2306 4 -0.35298182596191907 2306 5 -0.2990581262485037 2306 6 -0.18281271842727165 2306 7 -0.19344935543092134 2306 8 -0.15502159977842922 2306 9 -0.17880255052070027 2306 10-11 -0.21181635247650377 2306 12-13 -0.18973292273980036 2306 14-15 -0.2201196338883662 2306 16-17 -0.2158803063912913 2306 18-19 -0.2204865963015834 2306 20-21 -0.19514884187878323 2306 22-23 -0.1828981513986605 2306 24-25 -0.18318220539124042 2306 26-27 -0.19917295777230493 2306 28-29 -0.18366790207284112 2306 30-31 -0.10725294634443117 2306 32-33 -0.12474239035633872 2306 34-35 -0.18367206268582237 2306 36-37 -0.14366388062465774 2306 38-39 -0.17094053549597987 2306 40-41 -0.1327266721683884 2306 42-43 -0.17274229755661707 2306 44-45 -0.12075595853928434 2306 46-47 -0.09977819424940293 2306 48-49 -0.1064210678100963 2306 50-51 -0.10110488686427033 2306 52-53 -0.2052761810371777 2306 54-55 -0.2861167066390564 2306 56-57 -0.06976111968234733 2306 58-59 -0.05472841394375649 2306 60-61 -0.11593542308884963 2306 62-63 -0.08473125755107702 2306 64-65 -0.08407911509043942 2306 66-67 -0.13418207289218742 2306 68-69 -0.09582253338415825 2306 70-71 -0.1741086106614489 2306 72-73 -0.10182203851132954 2306 74-75 -0.09456604879509456 2306 76-77 -0.06543395270242769 2306 78-79 -0.1182419748563035 2306 80-81 -0.03989011178584434 2306 82-83 -0.09395088471378443 2306 84-85 0.0367748203286169 2306 86-87 -0.1461861273479208 2306 88-89 -0.11957344004320802 2306 90-91 -0.13756572649140963 2306 92-93 -0.2979278382004722 2306 94-95 -0.09697513849570072 2306 96-97 0.028590258075332997 2306 98-99 0.007664167756090023 2306 100-101 0.103230023405807 2307 1 -0.8827286327298651 2307 2 -1.3081502119596138 2307 3 -0.8209105859963088 2307 4 -0.47392048398829445 2307 5 -0.4194530109424335 2307 6 -0.4003327312306908 2307 7 -0.347207391983801 2307 8 -0.3384729093910792 2307 9 -0.41850517421607947 2307 10-11 -0.34031150942838195 2307 12-13 -0.5753355815465397 2307 14-15 -0.6161595361831829 2307 16-17 -0.5507762463248724 2307 18-19 -0.5421324616895333 2307 20-21 -0.48153480330526577 2307 22-23 -0.49632076026814076 2307 24-25 -0.46007541042746425 2307 26-27 -0.45406733892220075 2307 28-29 -0.41464544816921034 2307 30-31 -0.4349988112113863 2307 32-33 -0.4443364956442011 2307 34-35 -0.4895215363801739 2307 36-37 -0.3423877292637485 2307 38-39 -0.580435873163232 2307 40-41 -0.6092321835472063 2307 42-43 -0.42739771899164936 2307 44-45 -0.44124324561518335 2307 46-47 -0.4870616103518586 2307 48-49 -0.44231361988669704 2307 50-51 -0.39392533334590496 2307 52-53 -0.3536604099425986 2307 54-55 -0.3107128532802079 2307 56-57 -0.2713665203028697 2307 58-59 -0.28419430362222187 2307 60-61 -0.31141738650328676 2307 62-63 -0.41279118234997725 2307 64-65 -0.4646889105697767 2307 66-67 -0.319165173759302 2307 68-69 -0.29904315078568544 2307 70-71 -0.22866568013237298 2307 72-73 -0.11770388317388125 2307 74-75 -0.061623584054324 2307 76-77 -0.03777991517099366 2307 78-79 -0.03283057017421953 2307 80-81 -0.09500908387945373 2307 82-83 -0.1524411315873877 2307 84-85 -0.045006909539090145 2307 86-87 -0.06599790358937696 2307 88-89 -0.08665580890616909 2307 90-91 -0.06839970338888435 2307 92-93 -0.19830372205708713 2307 94-95 -0.24345286811085387 2307 96-97 -0.0854371326950698 2307 98-99 -0.17274575036524809 2307 100-101 -0.09582514750658078 2308 1 -0.5678031112689474 2308 2 -0.4153457295785472 2308 3 -0.5746552328024386 2308 4 -0.38214920578691647 2308 5 -0.40765104125527074 2308 6 -0.415310875711036 2308 7 -0.4274876075282279 2308 8 -0.42146799911675004 2308 9 -0.608498898403731 2308 10-11 -0.6899882693856085 2308 12-13 -0.6051005403396488 2308 14-15 -0.7147879110106672 2308 16-17 -0.8349921719027691 2308 18-19 -0.7655219810691207 2308 20-21 -0.7251282362454603 2308 22-23 -0.8719074473224282 2308 24-25 -0.983882700186058 2308 26-27 -0.950671098714885 2308 28-29 -0.9330686921196332 2308 30-31 -0.8507515288527188 2308 32-33 -0.8808237054206529 2308 34-35 -0.8278403128027279 2308 36-37 -0.9584706029567585 2308 38-39 -0.8596678686007451 2308 40-41 -0.7890370419273154 2308 42-43 -0.8791123763087612 2308 44-45 -0.8855562863215312 2308 46-47 -0.9557303233130767 2308 48-49 -1.0893917665439687 2308 50-51 -1.053429131757376 2308 52-53 -0.9883536603088956 2308 54-55 -0.8619105261375068 2308 56-57 -0.8409701212136511 2308 58-59 -0.8343998083593718 2308 60-61 -0.8763048247782805 2308 62-63 -0.8458807784059559 2308 64-65 -1.077343995658481 2308 66-67 -0.8783116899888626 2308 68-69 -0.8100076447215656 2308 70-71 -0.7892214905820794 2308 72-73 -0.6597625538026648 2308 74-75 -0.779493074791695 2308 76-77 -0.9925874423784578 2308 78-79 -1.0554908485082706 2308 80-81 -0.8226127737875615 2308 82-83 -0.7930302680519183 2308 84-85 -0.9057296477652699 2308 86-87 -0.8411635504264794 2308 88-89 -0.8773720828087299 2308 90-91 -0.8796410916841815 2308 92-93 -0.8976179132082827 2308 94-95 -0.7903101552894505 2308 96-97 -0.7140566086810125 2308 98-99 -0.8843631019151701 2308 100-101 -0.9448056268529257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 65.0 13 579.0 14 2933.0 15 7361.0 16 13058.0 17 18792.0 18 23928.0 19 29711.0 20 36143.0 21 39547.0 22 43466.0 23 49414.0 24 59664.0 25 75087.0 26 97465.0 27 126113.0 28 161362.0 29 205442.0 30 261964.0 31 333911.0 32 429086.0 33 569114.0 34 795322.0 35 1197273.0 36 1900608.0 37 2610712.0 38 2473639.0 39 481587.0 40 105.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.76035679977131 12.609094172669282 13.709966394208237 36.920582633351174 2 23.50806789452038 19.052787889119667 30.12603238583668 27.313111830523273 3 26.425471226863785 19.476809646666208 22.81770282550125 31.28001630096876 4 28.67605933188524 23.84038945683293 20.767123014686835 26.716428196594993 5 29.167051384585953 26.310720112233565 22.853614444225975 21.668614058954503 6 25.34495081810404 30.584235953533607 22.598945020432673 21.471868207929685 7 21.155762930507656 21.03800603414834 37.31721193650726 20.489019098836746 8 22.709934756269295 23.48781778253684 29.15137482131375 24.650872639880113 9 22.22440136784657 22.066273999755552 31.569127665680018 24.14019696671786 10-11 24.913687566093984 29.594021848877933 23.774923908884627 21.71736667614346 12-13 23.985884948639328 25.14592987262128 27.33012434304572 23.538060835693674 14-15 24.002645087921607 25.80958488991864 27.16626357085541 23.021506451304344 16-17 24.49346765579581 25.598810673613954 26.41980840051228 23.487913270077957 18-19 24.40572706040525 25.589095854213273 26.499893386084526 23.505283699296946 20-21 24.227933410476197 25.535664347509552 26.374563912551345 23.861838329462906 22-23 24.3410404787463 25.9002939023483 26.100950590926725 23.65771502797868 24-25 24.031590267776956 25.935715628470764 26.289957799488782 23.74273630426349 26-27 24.018163889169355 25.97768032697591 26.146875946572145 23.85727983728259 28-29 24.22531373054379 25.997815743255092 25.870240236689533 23.906630289511583 30-31 24.219229319033335 25.85848510389836 25.986783026429382 23.935502550638923 32-33 24.217385015501428 25.733554942845906 26.06353219346753 23.98552784818514 34-35 24.49898517501953 25.8198684829338 25.724858379521624 23.956287962525042 36-37 24.551977628015482 25.729015231550445 25.858857534700068 23.860149605734 38-39 24.26091802549881 25.87231877621511 25.88725687548023 23.97950632280585 40-41 24.35794373261708 26.148453745559348 25.571266576620523 23.92233594520305 42-43 24.2300187664308 26.11771362248909 25.892576237120135 23.759691373959974 44-45 24.393495125537417 25.941598617800327 25.99202158742469 23.67288466923757 46-47 24.499663785356717 25.98831245160012 25.984679606294232 23.52734415674893 48-49 24.303522178351464 25.96635882590512 26.182085939451266 23.548033056292148 50-51 24.006701232603 25.945928643738142 26.210860902385498 23.83650922127336 52-53 24.32428123644341 25.926632790757758 25.799628232153086 23.949457740645737 54-55 24.24353286360807 25.822503568773875 25.818468147406136 24.115495420211914 56-57 24.088982136955117 25.90269171450033 25.974365924658965 24.03396022388559 58-59 24.332525481057928 25.87652580787442 25.88188141344146 23.909067297626198 60-61 24.21730248704548 25.74590107716449 26.123123705606595 23.913672730183443 62-63 24.102569063264475 25.943049367046356 26.155045536227462 23.799336033461707 64-65 24.540919640775765 25.821287541923716 26.003772589296965 23.634020228003553 66-67 24.42449509781108 25.97973096820748 25.713411854322597 23.882362079658844 68-69 24.469205517087453 25.69586753171183 25.97892996827489 23.85599698292583 70-71 24.446363236599193 25.690657233272574 25.827818858639912 24.035160671488317 72-73 24.26457446483888 25.638927043553334 25.85280674291695 24.243691748690836 74-75 24.324645683099604 25.849083518883614 25.861575780241154 23.96469501777563 76-77 24.31907419265699 25.6470277302462 26.082322217144316 23.95157585995249 78-79 24.166356401351905 25.750826839073437 26.06955179642787 24.013264963146792 80-81 24.211011357537238 25.818083281078124 25.98008827366497 23.99081708771967 82-83 24.11578121761727 25.742361660971735 25.695879986608496 24.4459771348025 84-85 23.95428471314918 25.749417422160004 25.773496891049952 24.52280097364087 86-87 24.05731793265986 25.93050117839929 25.606702926468948 24.405477962471902 88-89 24.114436088777175 25.885489181842818 25.7144709957009 24.285603733679103 90-91 23.904230646086972 25.764693290696624 25.90301737308626 24.428058690130143 92-93 24.07931743180695 25.798263388847854 25.7965570680044 24.325862111340797 94-95 24.092390921675637 25.945617271321453 25.62115890986773 24.340832897135176 96-97 24.24488037320849 25.78508195654138 25.595912834322636 24.374124835927493 98-99 24.096023599870335 25.824846289968594 25.45650517592292 24.622624934238146 100-101 24.120008434457773 25.893581936443727 25.500829143401432 24.485580485697064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1955.0 1 1415.5 2 909.5 3 1131.5 4 1266.5 5 1071.5 6 880.5 7 860.5 8 816.0 9 681.5 10 665.5 11 672.0 12 689.5 13 775.5 14 890.0 15 1107.5 16 1455.0 17 1919.5 18 2638.5 19 3759.5 20 5371.0 21 7868.0 22 11564.0 23 16802.5 24 24568.5 25 34554.5 26 46308.0 27 61050.5 28 78947.5 29 98271.5 30 120167.0 31 143494.5 32 164526.0 33 186658.0 34 210903.5 35 234705.0 36 257902.5 37 282056.5 38 306208.0 39 325333.0 40 340909.0 41 356020.5 42 367965.0 43 375282.5 44 378292.0 45 382119.0 46 382212.5 47 372352.5 48 362702.0 49 354443.0 50 349566.0 51 359164.0 52 358222.0 53 342466.0 54 335055.5 55 323727.5 56 303473.5 57 294786.5 58 291743.5 59 290868.5 60 288259.0 61 284215.0 62 282995.0 63 275480.0 64 254123.5 65 213951.5 66 168767.0 67 136836.0 68 121516.0 69 103462.0 70 77726.5 71 56899.0 72 46702.0 73 38391.5 74 30031.5 75 24424.0 76 20092.0 77 16459.5 78 13093.5 79 9263.0 80 6226.0 81 3990.5 82 2697.0 83 1905.5 84 1014.0 85 567.5 86 379.5 87 257.0 88 166.0 89 106.0 90 71.5 91 56.5 92 46.0 93 30.0 94 19.5 95 11.5 96 4.5 97 1.5 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08150484379234664 2 0.004973655402568831 3 0.0 4 1.3285223112036943E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.490979333506927E-5 32-33 4.1516322225115446E-4 34-35 0.0 36-37 4.151632222511545E-6 38-39 0.0035247357569123017 40-41 0.006161022218207132 42-43 0.0011873668156383017 44-45 0.0027691386924152002 46-47 0.004600008502542791 48-49 0.0 50-51 0.0 52-53 0.0015568620834418292 54-55 8.635395022824013E-4 56-57 6.01986672264174E-4 58-59 0.0 60-61 0.0014115549556539251 62-63 6.476546267118011E-4 64-65 2.9061425557580814E-5 66-67 7.888101222771934E-5 68-69 0.0 70-71 0.0 72-73 2.9061425557580814E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 8.30326444502309E-6 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.0758161112557723E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2043456E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.90490788536302 #Duplication Level Percentage of deduplicated Percentage of total 1 84.16943144515349 54.63009194711065 2 9.336328746941385 12.119471146153945 3 2.85061431089968 5.550565777969238 4 1.2282810267767654 3.188858676011402 5 0.6409596855923351 2.0800714675800873 6 0.39652669421493525 1.544191713726473 7 0.2596518150666195 1.1796873997416375 8 0.18825330015142663 0.9774850484355153 9 0.13731586680766408 0.8021226317715192 >10 0.7259081090629305 8.3623863736325 >50 0.04132700507272537 1.8172343008579153 >100 0.020570582760073067 2.534582377937614 >500 0.0022464364608604237 1.0345462381156565 >1k 0.002341836740326547 3.0244090935851 >5k 2.3034154618560522E-4 1.0422184154134453 >10k+ 1.2796752565866955E-5 0.11207739195732482 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT 13494 0.11204425042114158 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 8.30326444502309E-6 0.0 8.30326444502309E-6 0.0 2 8.30326444502309E-6 1.660652889004618E-5 0.0 4.1516322225115446E-5 0.0 3 8.30326444502309E-6 1.660652889004618E-5 0.0 4.1516322225115446E-5 0.0 4 1.660652889004618E-5 3.321305778009236E-5 0.0 6.642611556018472E-5 0.0 5 4.1516322225115446E-5 4.1516322225115446E-5 0.0 7.472938000520781E-5 0.0 6 7.472938000520781E-5 6.642611556018472E-5 0.0 7.472938000520781E-5 0.0 7 9.133590889525399E-5 1.0794243778530016E-4 0.0 8.303264445023089E-5 0.0 8 1.1624570223032326E-4 1.3285223112036943E-4 0.0 8.303264445023089E-5 0.0 9 1.2454896667534634E-4 1.6606528890046178E-4 0.0 9.133590889525399E-5 0.0 10-11 1.6191365667795026E-4 1.7436855334548488E-4 0.0 9.963917334027708E-5 0.0 12-13 2.490979333506927E-4 1.9927834668055416E-4 0.0 1.1624570223032326E-4 0.0 14-15 4.5667954447626993E-4 2.2003650779311187E-4 0.0 1.3285223112036943E-4 0.0 16-17 5.231056600364546E-4 2.407946689056696E-4 0.0 1.3285223112036943E-4 0.0 18-19 5.853801433741278E-4 2.490979333506927E-4 0.0 1.3700386334288098E-4 0.0 20-21 7.348389033845434E-4 2.5740119779571577E-4 0.0 1.5361039223292717E-4 4.151632222511545E-6 22-23 0.001287005988978579 2.7400772668576196E-4 0.0 1.5776202445543872E-4 8.30326444502309E-6 24-25 0.0019305089834678684 3.030691522433428E-4 0.0 1.7021692112297333E-4 8.30326444502309E-6 26-27 0.003130330695773705 3.4458547446845824E-4 0.0 1.9927834668055416E-4 8.30326444502309E-6 28-29 0.0051023560014666885 3.7364690002603904E-4 0.0 1.9927834668055416E-4 8.30326444502309E-6 30-31 0.0075186059549684076 3.8610179669357366E-4 0.0 2.2418814001562342E-4 8.30326444502309E-6 32-33 0.011130525988553451 4.1516322225115446E-4 0.0 2.2418814001562342E-4 8.30326444502309E-6 34-35 0.015705624697761173 4.2346648669617756E-4 0.0 2.3249140446064651E-4 1.2454896667534635E-5 36-37 0.021003107413685905 4.525279122537584E-4 0.0 2.490979333506927E-4 1.660652889004618E-5 38-39 0.0265040201085137 4.815893378113392E-4 0.0 2.6985609446325044E-4 1.660652889004618E-5 40-41 0.03163958916776048 4.940442344788738E-4 0.0 2.8231099113078505E-4 1.660652889004618E-5 42-43 0.03582858608027463 4.981958667013854E-4 8.30326444502309E-6 2.8231099113078505E-4 1.660652889004618E-5 44-45 0.0398805791294459 5.272572922589662E-4 8.30326444502309E-6 2.9891752002083124E-4 1.660652889004618E-5 46-47 0.04433528050420078 5.687736144840816E-4 8.30326444502309E-6 3.1137241668836586E-4 2.490979333506927E-5 48-49 0.04979882850902598 6.01986672264174E-4 8.30326444502309E-6 3.362822100234351E-4 2.9061425557580814E-5 50-51 0.05522501182384857 6.144415689317087E-4 8.30326444502309E-6 3.611920033585044E-4 3.321305778009236E-5 52-53 0.06264813023769922 6.351997300442664E-4 8.30326444502309E-6 3.7364690002603904E-4 3.321305778009236E-5 54-55 0.07485808060410568 6.559578911568241E-4 8.30326444502309E-6 3.8195016447106214E-4 3.321305778009236E-5 56-57 0.08284582100021787 6.767160522693818E-4 1.660652889004618E-5 4.0270832558361985E-4 3.321305778009236E-5 58-59 0.09017345187295076 7.306872711620319E-4 1.660652889004618E-5 4.1101159002864294E-4 3.321305778009236E-5 60-61 0.09694891566008959 7.389905356070549E-4 1.660652889004618E-5 4.732860733663161E-4 3.321305778009236E-5 62-63 0.10524387684066766 7.431421678295665E-4 1.660652889004618E-5 4.981958667013854E-4 3.321305778009236E-5 64-65 0.11309046174121448 7.555970644971011E-4 1.660652889004618E-5 5.064991311464085E-4 3.321305778009236E-5 66-67 0.12148921372735534 7.555970644971011E-4 1.660652889004618E-5 5.272572922589662E-4 3.321305778009236E-5 68-69 0.1300623342668417 7.680519611646358E-4 1.660652889004618E-5 5.521670855940354E-4 3.321305778009236E-5 70-71 0.13907137618969173 7.971133867222167E-4 1.660652889004618E-5 5.853801433741278E-4 3.321305778009236E-5 72-73 0.15636292439645233 8.593878700598898E-4 1.660652889004618E-5 5.895317755966393E-4 3.321305778009236E-5 74-75 0.16707828716275464 8.801460311724475E-4 2.0758161112557723E-5 5.978350400416625E-4 4.981958667013854E-5 76-77 0.17939202833472384 8.926009278399822E-4 2.490979333506927E-5 6.102899367091972E-4 4.981958667013854E-5 78-79 0.195446390139176 9.133590889525399E-4 2.490979333506927E-5 6.227448333767317E-4 4.981958667013854E-5 80-81 0.21199894781032952 9.133590889525399E-4 2.490979333506927E-5 6.47654626711801E-4 4.981958667013854E-5 82-83 0.23120855010389046 9.299656178425861E-4 2.490979333506927E-5 6.559578911568241E-4 4.981958667013854E-5 84-85 0.25277627949983794 9.548754111776553E-4 2.490979333506927E-5 6.93322581159428E-4 4.981958667013854E-5 86-87 0.28025178154841934 9.6733030784519E-4 2.490979333506927E-5 7.555970644971012E-4 4.981958667013854E-5 88-89 0.31548253258865233 9.714819400677015E-4 2.490979333506927E-5 7.929617544997051E-4 4.981958667013854E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGAT 4450 0.0 31.940397 1 GGCGATC 5050 0.0 28.124466 2 GCGATCT 5005 0.0 27.521563 3 CGATCTG 5355 0.0 25.190575 4 GGCGCGT 6295 0.0 23.013535 5 TGGCGCG 7680 0.0 18.801458 4 GCGCGTG 8135 0.0 17.866648 6 CGGTGGC 8685 0.0 17.679169 1 CGCGTGC 8255 0.0 17.664465 7 GCGACGC 2115 0.0 17.306509 1 CGACGCT 2130 0.0 16.948778 2 GCGTGCC 9115 0.0 16.049934 8 CTCGCTA 3950 0.0 15.885686 1 GTGCGTT 4060 0.0 15.85382 42-43 AAGTGCG 4145 0.0 15.700682 40-41 GTGGCGC 9355 0.0 15.587403 3 GCGTTCG 4320 0.0 15.3394985 44-45 GGTGGCG 9555 0.0 15.311737 2 CGTGCCT 9580 0.0 15.270892 9 CCGCGCT 3320 0.0 15.166041 2 >>END_MODULE