FastQCFastQC Report
Thu 26 May 2016
SRR1562287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15738995
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC301980.19186739687000345No Hit
GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC216460.1375310177047518No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG197920.12575135833005857No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG159340.1012389927056969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGC50750.047.7286831
CGACGCT71650.034.7344472
GACGCTC90200.028.0650623
GGGCGAT53650.027.708941
GGCGCGT78650.024.8796235
ACGCTCA108300.023.8569954
GGCGATC63750.023.6914832
GCGATCT60800.023.4348953
CGATCTG67400.021.421954
TCGCAGC131650.021.12393492-93
ACCGCGT49100.020.1199658
ACGGGGT61800.019.9047432
CGCGTGC99050.019.515757
GACCGCG51300.019.0719537
GCGCGTG102150.018.969996
AGACCGC54250.018.5601466
TGGCGCG104000.018.495544
AAGTGCG148550.018.0173740-41
GTGCGTT149300.018.0065142-43
GCGTGCC108150.017.9175648
Produced by FastQC (version 0.11.5)