FastQCFastQC Report
Thu 26 May 2016
SRR1562287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15738995
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC324820.20637912395295885No Hit
GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC238850.15175683072521468No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG218520.13883986874638438No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG167230.10625201926806635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGC51600.046.240871
CGACGCT74300.033.0534672
GACGCTC95500.025.9631633
GGGCGAT57950.025.180071
GCGATCT62150.023.3103713
ACGGGGT64650.022.9980562
CGATCTG65250.022.7124084
GGCGATC66100.022.7091522
ACGCTCA113650.022.5272624
ACCGCGT52700.021.5414528
AGACCGC53950.021.4825636
CCGCGTT56300.020.4171319
TCGCAGC139400.020.20596992-93
GACCGCG56150.020.1332997
GGCGCGT66950.019.0139315
GTCTCGC93750.018.5437497
GACGGGG74350.018.5391181
CTCGCTA94700.018.0567789
AAGTGCG156350.017.80296140-41
CAGACCG65300.017.5303925
Produced by FastQC (version 0.11.5)