##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1562287_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15738995 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.905121705674347 31.0 31.0 34.0 30.0 34.0 2 31.83247774079603 33.0 31.0 34.0 30.0 34.0 3 31.94022324805364 34.0 31.0 34.0 30.0 34.0 4 35.905884079637865 37.0 35.0 37.0 35.0 37.0 5 35.99569159276053 37.0 35.0 37.0 35.0 37.0 6 36.01784986906724 37.0 35.0 37.0 35.0 37.0 7 35.99871948621878 37.0 35.0 37.0 35.0 37.0 8 36.0158553325673 37.0 35.0 37.0 35.0 37.0 9 37.76294051812076 39.0 37.0 39.0 35.0 39.0 10-11 37.77978991034688 39.0 37.0 39.0 35.0 39.0 12-13 37.71703644355945 39.0 37.0 39.0 35.0 39.0 14-15 39.01255982354655 40.0 38.0 41.0 36.0 41.0 16-17 38.95693759353758 40.0 38.0 41.0 36.0 41.0 18-19 38.97425086544598 40.0 38.0 41.0 36.0 41.0 20-21 38.93769786444433 40.0 38.0 41.0 35.0 41.0 22-23 38.838055479400055 40.0 38.0 41.0 35.0 41.0 24-25 38.708249637286244 40.0 38.0 41.0 35.0 41.0 26-27 38.52920853586903 40.0 38.0 41.0 34.0 41.0 28-29 38.4688143366206 40.0 38.0 41.0 34.0 41.0 30-31 38.342904010071805 40.0 38.0 41.0 34.0 41.0 32-33 38.11487725232774 40.0 38.0 41.0 33.5 41.0 34-35 38.00111284106768 40.0 37.0 41.0 33.0 41.0 36-37 37.99781809448443 40.0 37.0 41.0 33.0 41.0 38-39 38.10552147707017 40.0 37.0 41.0 33.0 41.0 40-41 38.05310980783716 40.0 37.0 41.0 33.0 41.0 42-43 37.92075122966873 40.0 37.0 41.0 33.0 41.0 44-45 37.755878154863126 40.0 37.0 41.0 33.0 41.0 46-47 37.63052192976744 39.0 36.0 41.0 33.0 41.0 48-49 37.43468858716837 39.0 36.0 41.0 32.0 41.0 50-51 37.22016145249427 39.0 35.0 40.0 32.0 41.0 52-53 36.98067227291196 39.0 35.0 40.0 31.5 41.0 54-55 36.68983286416953 38.0 35.0 40.0 31.0 41.0 56-57 36.44668582714462 38.0 35.0 40.0 31.0 41.0 58-59 36.13357368116579 38.0 34.5 40.0 30.5 41.0 60-61 35.85574364818084 37.0 34.0 40.0 30.0 41.0 62-63 35.57935173116199 37.0 34.0 40.0 30.0 41.0 64-65 35.423112022082734 36.0 34.0 39.5 30.0 41.0 66-67 35.174617280201176 36.0 34.0 39.0 29.5 41.0 68-69 34.82702221456961 35.0 34.0 39.0 29.0 40.5 70-71 34.465822055347246 35.0 33.5 38.5 29.0 40.0 72-73 34.18673784444306 35.0 33.0 37.5 29.0 40.0 74-75 33.875569437565744 35.0 33.0 37.0 29.0 39.5 76-77 33.11166545259084 35.0 32.5 36.0 27.0 39.0 78-79 33.311743094142926 35.0 33.0 36.0 28.5 39.0 80-81 33.133907056962656 35.0 33.0 36.0 28.0 37.5 82-83 32.902284707505146 35.0 33.0 35.5 28.0 37.0 84-85 32.616052009674064 35.0 33.0 35.0 27.0 37.0 86-87 32.36016009916771 35.0 33.0 35.0 27.0 36.0 88-89 32.16567843118318 35.0 33.0 35.0 27.0 36.0 90-91 31.94883548790758 35.0 32.0 35.0 26.0 36.0 92-93 31.760546559675507 34.0 32.0 35.0 26.0 35.5 94-95 31.508684163124773 34.0 32.0 35.0 25.0 35.0 96-97 31.30539198976809 34.0 32.0 35.0 25.0 35.0 98-99 31.037541532988605 34.0 32.0 35.0 24.0 35.0 100-101 30.564807505180603 34.0 31.5 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4551537041384286 1101 2 0.8101732939035386 1101 3 0.8940551934720737 1101 4 0.4537206843811674 1101 5 0.34982394468514144 1101 6 0.3014505403555461 1101 7 0.298955622592878 1101 8 0.27282845383260224 1101 9 0.37915084966615353 1101 10-11 0.3752390272576065 1101 12-13 0.3713195161782963 1101 14-15 0.4848908911963292 1101 16-17 0.5094337101614173 1101 18-19 0.47011884953313654 1101 20-21 0.4862776406124425 1101 22-23 0.4857668730220297 1101 24-25 0.5015720565622672 1101 26-27 0.5042019142305278 1101 28-29 0.49025656958092867 1101 30-31 0.466991411474865 1101 32-33 0.45982710857147424 1101 34-35 0.4857366370021552 1101 36-37 0.48951875480177165 1101 38-39 0.5669614077603953 1101 40-41 0.5207252754643363 1101 42-43 0.518997643088035 1101 44-45 0.5584308454116496 1101 46-47 0.5294009116673379 1101 48-49 0.5204911609005549 1101 50-51 0.5233134896395413 1101 52-53 0.5102634128790626 1101 54-55 0.5413387748538554 1101 56-57 0.4881675119183271 1101 58-59 0.5202776842672208 1101 60-61 0.50199205981653 1101 62-63 0.4891528715030802 1101 64-65 0.46889251066678383 1101 66-67 0.49326978992035464 1101 68-69 0.37675974867430284 1101 70-71 0.3379841704535238 1101 72-73 0.3230299426197618 1101 74-75 0.27041093693382834 1101 76-77 0.2127534350987048 1101 78-79 0.22159400869202273 1101 80-81 0.28648743269963717 1101 82-83 0.2601371299488946 1101 84-85 0.23138178837335488 1101 86-87 0.20881079933705138 1101 88-89 0.18498898437214706 1101 90-91 0.1870742832560417 1101 92-93 0.16316558211147125 1101 94-95 0.15160243611321533 1101 96-97 0.18270598082320788 1101 98-99 0.02928314097371043 1101 100-101 -0.04659870362307572 1102 1 0.4241228247992872 1102 2 0.7628545095224695 1102 3 0.8608834301015342 1102 4 0.42245677582349117 1102 5 0.3118495123479619 1102 6 0.2837714662586137 1102 7 0.26380228492408975 1102 8 0.2275040145028342 1102 9 0.3183602764824016 1102 10-11 0.31287609639962 1102 12-13 0.3094831481904947 1102 14-15 0.40673569380514607 1102 16-17 0.40479236964109333 1102 18-19 0.36767327538400707 1102 20-21 0.3944261580225614 1102 22-23 0.3992211370705263 1102 24-25 0.38082669389497426 1102 26-27 0.46035515697877116 1102 28-29 0.4326432424194806 1102 30-31 0.39671257888529254 1102 32-33 0.45282705552899216 1102 34-35 0.4312698080819928 1102 36-37 0.4871197899140256 1102 38-39 0.43143547625073353 1102 40-41 0.47446751167811385 1102 42-43 0.5024098019295025 1102 44-45 0.4842297511415339 1102 46-47 0.5288158607987299 1102 48-49 0.5368426292045001 1102 50-51 0.48922188458349325 1102 52-53 0.5532137225054541 1102 54-55 0.563318533905175 1102 56-57 0.5410533079381636 1102 58-59 0.5568352408551007 1102 60-61 0.5535827978056531 1102 62-63 0.5908538629008575 1102 64-65 0.56043199840488 1102 66-67 0.6420259823685228 1102 68-69 0.5614578813372972 1102 70-71 0.603440700610065 1102 72-73 0.5328872904599393 1102 74-75 0.45940352297053977 1102 76-77 0.497315934617383 1102 78-79 0.4833762416740299 1102 80-81 0.5401316770226217 1102 82-83 0.5095472202701288 1102 84-85 0.5346643093751453 1102 86-87 0.5781502456345677 1102 88-89 0.4994114640327041 1102 90-91 0.5217970881937184 1102 92-93 0.5674241178845953 1102 94-95 0.6310875029446947 1102 96-97 0.6955452015306633 1102 98-99 0.6985526261011472 1102 100-101 0.6721447871092749 1103 1 0.34620265758492863 1103 2 0.7070669569086405 1103 3 0.7864285591859392 1103 4 0.37041460546461025 1103 5 0.262532984727855 1103 6 0.22077106589646434 1103 7 0.24139669073930747 1103 8 0.2102496304851158 1103 9 0.22969366948440495 1103 10-11 0.22516365145322936 1103 12-13 0.22665539678408209 1103 14-15 0.32203286402739195 1103 16-17 0.31904548559622725 1103 18-19 0.30993329170988204 1103 20-21 0.3192648832331244 1103 22-23 0.31679900302539465 1103 24-25 0.33142687880503274 1103 26-27 0.37318428310097573 1103 28-29 0.3392593003297506 1103 30-31 0.3117611057402101 1103 32-33 0.3122064389861947 1103 34-35 0.36804626425196574 1103 36-37 0.3801062026879478 1103 38-39 0.25552081384899594 1103 40-41 0.28043361527597455 1103 42-43 0.24073876921073634 1103 44-45 0.26156347105697364 1103 46-47 0.24403818691520485 1103 48-49 0.2500104647840473 1103 50-51 0.13092461289603818 1103 52-53 0.20849509276607847 1103 54-55 0.28346809576113685 1103 56-57 0.23446862799282542 1103 58-59 0.15980809811723873 1103 60-61 0.08293940043753878 1103 62-63 0.06412847412506295 1103 64-65 -0.15347836830688522 1103 66-67 -0.0036934758032316495 1103 68-69 -0.11781573485700392 1103 70-71 -0.11709019718500002 1103 72-73 -0.19029833548675867 1103 74-75 -0.2643510753788618 1103 76-77 -0.13056327114154698 1103 78-79 -0.14467204376647658 1103 80-81 -0.11862368213224528 1103 82-83 -0.17894504765319397 1103 84-85 -0.13063610475336418 1103 86-87 -0.04712074086092599 1103 88-89 -0.08631133743283215 1103 90-91 -0.06427034689003719 1103 92-93 -0.016189135043518377 1103 94-95 0.1721939063273119 1103 96-97 0.2523840882061279 1103 98-99 0.21206789762719325 1103 100-101 0.26678221355681586 1104 1 0.2457055474556462 1104 2 0.5835822805480362 1104 3 0.7041031580267862 1104 4 0.3252465904075592 1104 5 0.20355221015493896 1104 6 0.13211167091641585 1104 7 0.16035773962184408 1104 8 0.08806684667035114 1104 9 0.15318730496560562 1104 10-11 0.12119641451317165 1104 12-13 0.13314612585157448 1104 14-15 0.1541482880286793 1104 16-17 0.091490694171803 1104 18-19 0.11283983055793811 1104 20-21 0.15497474615256124 1104 22-23 0.16982875369858164 1104 24-25 0.07631440072887585 1104 26-27 0.07087153867573903 1104 28-29 0.07807437239656423 1104 30-31 -0.01916900280171774 1104 32-33 0.05240516595188893 1104 34-35 0.11976123485946033 1104 36-37 0.11338903971606129 1104 38-39 -0.15842526727855244 1104 40-41 -0.13688400684282698 1104 42-43 -0.12285118406843054 1104 44-45 -0.20649301914693297 1104 46-47 -0.23351468707260636 1104 48-49 -0.18789414952879469 1104 50-51 0.11337198454409503 1104 52-53 0.025832661427237724 1104 54-55 0.10759618205283061 1104 56-57 0.14333732625375717 1104 58-59 -0.07682155771871635 1104 60-61 -0.16958859185243114 1104 62-63 -0.05231929756963183 1104 64-65 -0.08336514236925296 1104 66-67 -0.10410097942787644 1104 68-69 -0.0933187217255309 1104 70-71 0.03477934951308015 1104 72-73 0.12588776965051096 1104 74-75 0.16575146486809444 1104 76-77 0.22739752424771353 1104 78-79 0.25268649361434115 1104 80-81 0.20807723743853757 1104 82-83 0.17772157185269322 1104 84-85 0.19625187401811672 1104 86-87 0.40605774924619453 1104 88-89 0.2850366255869474 1104 90-91 0.31011964002912507 1104 92-93 0.3577439287700237 1104 94-95 0.3376741458293395 1104 96-97 0.3774539986590639 1104 98-99 0.44715642908223785 1104 100-101 0.5184339199750454 1105 1 0.1534607455322181 1105 2 0.48771490630497283 1105 3 0.5647730554492973 1105 4 0.25680943394723954 1105 5 0.1115069083936433 1105 6 0.06824453130189312 1105 7 0.045499228537622116 1105 8 -0.0012589390057087257 1105 9 -0.013735377023095907 1105 10-11 -0.09047862891947034 1105 12-13 -0.009870000905834786 1105 14-15 0.046453345328131945 1105 16-17 -0.0013659049534808787 1105 18-19 0.02509806760130573 1105 20-21 0.05477882143932078 1105 22-23 0.04683876561454525 1105 24-25 0.05824116564954096 1105 26-27 0.07028847687880813 1105 28-29 0.07118064330553153 1105 30-31 0.09578087171156824 1105 32-33 0.10722763914755262 1105 34-35 0.11386965301496588 1105 36-37 0.15088142747003275 1105 38-39 0.125255988537738 1105 40-41 0.13586210926402487 1105 42-43 0.11735605685816353 1105 44-45 0.11849416196256612 1105 46-47 0.13461196554271027 1105 48-49 0.17391021970270515 1105 50-51 0.18936900752978403 1105 52-53 0.19261843832449443 1105 54-55 0.21553351382548414 1105 56-57 0.23526308938965457 1105 58-59 0.30636363339808526 1105 60-61 0.29008479336280857 1105 62-63 0.25886833144311083 1105 64-65 0.24647380754941395 1105 66-67 0.37650344608810826 1105 68-69 0.32897553672966495 1105 70-71 0.3774288362302016 1105 72-73 0.42863234683144213 1105 74-75 0.3576456586027277 1105 76-77 0.45930713689973146 1105 78-79 0.44268728120169953 1105 80-81 0.4421941542877619 1105 82-83 0.4561189403223054 1105 84-85 0.3951636029404284 1105 86-87 0.5118519042275622 1105 88-89 0.5638134694640655 1105 90-91 0.5259669192608207 1105 92-93 0.6053201698627113 1105 94-95 0.6629911250264939 1105 96-97 0.7481009967319494 1105 98-99 0.730054134336072 1105 100-101 0.7448120330870864 1106 1 0.011321095059233954 1106 2 0.33571207374658485 1106 3 0.4468958709054469 1106 4 0.17207719088165874 1106 5 -0.05847866159192705 1106 6 -0.05549685077281907 1106 7 -0.08237074099901065 1106 8 -0.07647405310971322 1106 9 -0.13769454542671156 1106 10-11 -0.12390482206788533 1106 12-13 -0.16798020346048048 1106 14-15 -0.25440243469431323 1106 16-17 -0.21397813839904956 1106 18-19 -0.2633058124692411 1106 20-21 -0.19531203420708465 1106 22-23 -0.17444082927615767 1106 24-25 -0.1607850755989233 1106 26-27 -0.25373215269738125 1106 28-29 -0.23571970942816023 1106 30-31 -0.20392919188739 1106 32-33 -0.2711580697937137 1106 34-35 -0.2238447930328178 1106 36-37 -0.2280467431247999 1106 38-39 -0.22965355664958764 1106 40-41 -0.28701681222600683 1106 42-43 -0.31577685960808566 1106 44-45 -0.3315773200533556 1106 46-47 -0.37217882597030183 1106 48-49 -0.3026871027279512 1106 50-51 -0.2917764416712245 1106 52-53 -0.2583008070391912 1106 54-55 -0.22007187872434741 1106 56-57 -0.2184459587638301 1106 58-59 -0.20654722117943436 1106 60-61 -0.18556455728654697 1106 62-63 -0.28666638075826967 1106 64-65 -0.2960219168907514 1106 66-67 -0.11876203945515584 1106 68-69 -0.22684122880932733 1106 70-71 -0.3061870848857211 1106 72-73 -0.19250829236475653 1106 74-75 -0.14062674800301522 1106 76-77 -0.07112683242312556 1106 78-79 -0.11185884139997881 1106 80-81 -0.13464032810227167 1106 82-83 -0.13029613637054638 1106 84-85 -0.09475476058904064 1106 86-87 -0.03303946983047723 1106 88-89 -0.04192780572164878 1106 90-91 -0.20842127484960216 1106 92-93 -0.05465056927469192 1106 94-95 -0.047794642703721024 1106 96-97 0.02927316818189496 1106 98-99 0.10647108158635277 1106 100-101 0.12518120095625562 1107 1 -0.1152689889560321 1107 2 0.19164270087446766 1107 3 0.32575510795751583 1107 4 0.10754521833581521 1107 5 -0.05529848442013474 1107 6 -0.3581700844352511 1107 7 -0.2954117452550946 1107 8 -0.24831521089644326 1107 9 -0.2303530084754044 1107 10-11 -0.3328949452473893 1107 12-13 -0.33861715987762864 1107 14-15 -0.43306856588071696 1107 16-17 -0.5397264666210404 1107 18-19 -0.47700643084252903 1107 20-21 -0.4808801951146151 1107 22-23 -0.4654808128639232 1107 24-25 -0.43748192962837607 1107 26-27 -0.48822177953648094 1107 28-29 -0.5430309160320022 1107 30-31 -0.33757482370336334 1107 32-33 -0.3116563147768403 1107 34-35 -0.2835427813717857 1107 36-37 -0.46212327376238704 1107 38-39 -0.4753687032863496 1107 40-41 -0.4135330603124743 1107 42-43 -0.4936820544781426 1107 44-45 -0.5514520207644296 1107 46-47 -0.5166905680513665 1107 48-49 -0.47297611999986344 1107 50-51 -0.4836376189117928 1107 52-53 -0.32509909654334024 1107 54-55 -0.6123079066232577 1107 56-57 -0.5984533010305668 1107 58-59 -0.37380849916592496 1107 60-61 -0.4597679429807613 1107 62-63 -0.2593352377337723 1107 64-65 -0.308156625713778 1107 66-67 -0.19582794819793037 1107 68-69 -0.17089394743319275 1107 70-71 -0.24796700996266452 1107 72-73 -0.10032908582805788 1107 74-75 -0.15531927473886498 1107 76-77 -0.11819869830195273 1107 78-79 -0.04515699418122665 1107 80-81 -0.014756865866395685 1107 82-83 0.056239468199272835 1107 84-85 -0.030679915829864512 1107 86-87 0.05051437793809299 1107 88-89 0.1397206021756361 1107 90-91 0.11168937418562663 1107 92-93 0.1066779726171383 1107 94-95 0.17510505072251448 1107 96-97 0.14915182782392122 1107 98-99 0.2279332587658871 1107 100-101 0.22159565309734575 1108 1 -1.201151418856309 1108 2 -4.601590551524154 1108 3 -5.874661034225252 1108 4 -2.5022032323676413 1108 5 -1.424703960694444 1108 6 -0.9875421416679515 1108 7 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2305 5 0.06607437036704766 2305 6 0.02288651079397397 2305 7 0.03607798300911469 2305 8 0.021400876813707725 2305 9 0.005368782373388115 2305 10-11 -0.008901924798237815 2305 12-13 -0.019790815946784335 2305 14-15 -0.07258352750889685 2305 16-17 -0.0437933736970848 2305 18-19 -0.061853330939996454 2305 20-21 -0.006963800222365535 2305 22-23 0.012031016745496004 2305 24-25 0.0739316063388884 2305 26-27 -0.0989546685843905 2305 28-29 -0.013090277291745167 2305 30-31 -0.06348779447656483 2305 32-33 0.04016740377561945 2305 34-35 0.1072374631202706 2305 36-37 0.12598787857800886 2305 38-39 0.052127810503556304 2305 40-41 0.08920423595507287 2305 42-43 0.027104325349924352 2305 44-45 0.06609188566008584 2305 46-47 0.142825510149045 2305 48-49 0.1158890494063769 2305 50-51 0.14320983799368747 2305 52-53 0.1557458830946743 2305 54-55 0.23720505013210413 2305 56-57 0.20087615097140343 2305 58-59 0.243215291765857 2305 60-61 0.21185612794629094 2305 62-63 0.1408921958745637 2305 64-65 0.17917375359466803 2305 66-67 0.24165509170800448 2305 68-69 0.25301573075294215 2305 70-71 0.2944727794288511 2305 72-73 0.3427069252424033 2305 74-75 0.2511389108888835 2305 76-77 0.36066311751599045 2305 78-79 0.25075715951822275 2305 80-81 0.2960333200913041 2305 82-83 0.30643078379100785 2305 84-85 0.35793705027714395 2305 86-87 0.2934404033244604 2305 88-89 0.3114651943950051 2305 90-91 0.31928352290280415 2305 92-93 0.33298916949422264 2305 94-95 0.35097810209508395 2305 96-97 0.2946646032008928 2305 98-99 0.37342860308281445 2305 100-101 0.552117120533179 2306 1 -0.0023125035016846596 2306 2 0.27423146579691604 2306 3 0.194381373943596 2306 4 0.04699124537575017 2306 5 -0.0543646222388503 2306 6 -0.0869375336627698 2306 7 -0.10108915939008511 2306 8 -0.1285251636459961 2306 9 -0.18098773001924684 2306 10-11 -0.14779104697093715 2306 12-13 -0.18883420834316667 2306 14-15 -0.24918446313942866 2306 16-17 -0.21714773046139868 2306 18-19 -0.20907944144273927 2306 20-21 -0.1883987229652817 2306 22-23 -0.19359721357471216 2306 24-25 -0.19030948308618179 2306 26-27 -0.27728137832620803 2306 28-29 -0.20184674822463222 2306 30-31 -0.1374487273349132 2306 32-33 -0.1319852598641731 2306 34-35 -0.13305011978552983 2306 36-37 -0.1030174224967979 2306 38-39 -0.1724958138984718 2306 40-41 -0.22527059576962216 2306 42-43 -0.23254631592417496 2306 44-45 -0.17854291040261216 2306 46-47 -0.35254830432347006 2306 48-49 -0.36401001281741685 2306 50-51 -0.23341440994854423 2306 52-53 -0.23565588096578693 2306 54-55 -0.34076569833909076 2306 56-57 -0.17267893735384376 2306 58-59 -0.11479343800707653 2306 60-61 -0.20580207486972313 2306 62-63 -0.18523526123946965 2306 64-65 -0.16962424008935528 2306 66-67 -0.17420612534472468 2306 68-69 -0.1648055464658924 2306 70-71 -0.26238502934332786 2306 72-73 -0.18330684947954268 2306 74-75 -0.09524787434887827 2306 76-77 -0.07570226889435361 2306 78-79 -0.1609826500933238 2306 80-81 -0.16730948335231233 2306 82-83 -0.13823058688098655 2306 84-85 -0.04483618103931519 2306 86-87 -0.20928090865415072 2306 88-89 -0.099507816486188 2306 90-91 -0.14289020533264818 2306 92-93 -0.1588411865221353 2306 94-95 -0.10549486850712952 2306 96-97 -0.1823045596590056 2306 98-99 -0.1386628655519644 2306 100-101 -0.04057855770269825 2307 1 -0.874494804166158 2307 2 -4.493469613020594 2307 3 -5.300108632444001 2307 4 -2.2976930186667914 2307 5 -1.251231462880284 2307 6 -0.7659074158314425 2307 7 -0.5314316804608836 2307 8 -0.3880012237885211 2307 9 -0.5168491159343134 2307 10-11 -0.4601414866595306 2307 12-13 -0.4175624044302566 2307 14-15 -0.5971956919757062 2307 16-17 -0.5836701427912274 2307 18-19 -0.5046398606694282 2307 20-21 -0.5078938750199029 2307 22-23 -0.7283760883306911 2307 24-25 -0.6277164600284948 2307 26-27 -0.4792807300191555 2307 28-29 -0.4509512300068579 2307 30-31 -0.49562346042429795 2307 32-33 -0.5782420356627682 2307 34-35 -0.6924031179223462 2307 36-37 -0.6598914219847316 2307 38-39 -0.7807212324991397 2307 40-41 -0.7663050811125558 2307 42-43 -0.6029467621204176 2307 44-45 -0.4898756410292293 2307 46-47 -0.496073185987008 2307 48-49 -0.5108966443111456 2307 50-51 -0.5326435974874286 2307 52-53 -0.5315754760351439 2307 54-55 -0.41257589276324325 2307 56-57 -0.489455084441623 2307 58-59 -0.3447902744375497 2307 60-61 -0.618390055775734 2307 62-63 -0.5773039853311488 2307 64-65 -0.7016681330450183 2307 66-67 -0.513293153412441 2307 68-69 -0.40948467340282235 2307 70-71 -0.4035983556813747 2307 72-73 -0.31462717758012815 2307 74-75 -0.2970658383286917 2307 76-77 -0.200554024927861 2307 78-79 -0.3449431391115496 2307 80-81 -0.4709460063623112 2307 82-83 -0.5112349111092414 2307 84-85 -0.8442561073835364 2307 86-87 -0.5185865373056231 2307 88-89 -0.276098139134227 2307 90-91 -0.28839741252404494 2307 92-93 -0.3541883385817748 2307 94-95 -0.29086850415398047 2307 96-97 -0.22117537719748626 2307 98-99 -0.1881065268083688 2307 100-101 -0.3069843316596348 2308 1 -1.9585415650484634 2308 2 -4.1814420409178865 2308 3 -4.791756158009477 2308 4 -2.13870104161753 2308 5 -1.1717866640412353 2308 6 -0.7751477627190582 2308 7 -0.6447738920541113 2308 8 -0.6501596638006149 2308 9 -0.6871045991588076 2308 10-11 -0.6481673249362601 2308 12-13 -0.7349396738240088 2308 14-15 -0.9060068046566627 2308 16-17 -0.9145647787038556 2308 18-19 -0.7735807133606585 2308 20-21 -0.7155261690268802 2308 22-23 -1.1151808803301861 2308 24-25 -0.9281642584910372 2308 26-27 -0.9241491895560543 2308 28-29 -1.006485776334749 2308 30-31 -0.8233419727268085 2308 32-33 -1.0296604823002653 2308 34-35 -1.1309807145850854 2308 36-37 -1.126613108786465 2308 38-39 -0.9196924733905334 2308 40-41 -0.943554089871661 2308 42-43 -0.82916144087379 2308 44-45 -0.7928428511413657 2308 46-47 -0.8963657528665436 2308 48-49 -1.0182018228165433 2308 50-51 -0.7793725000898988 2308 52-53 -0.8905214467287479 2308 54-55 -0.9491254460208793 2308 56-57 -0.7740947788361368 2308 58-59 -0.7621296416746972 2308 60-61 -0.8693671819557238 2308 62-63 -0.9305666323167614 2308 64-65 -0.8097072492091044 2308 66-67 -0.6301363671193059 2308 68-69 -0.7164979323662308 2308 70-71 -0.8030482253513043 2308 72-73 -0.8383654367734792 2308 74-75 -0.8000881719700814 2308 76-77 -0.9240746024804878 2308 78-79 -0.7617963389460627 2308 80-81 -0.665052157756854 2308 82-83 -0.6017969069694047 2308 84-85 -0.4940462099465748 2308 86-87 -0.6099978114391256 2308 88-89 -0.5573407551573197 2308 90-91 -0.531422678850074 2308 92-93 -0.6057229988136861 2308 94-95 -0.5973036102777698 2308 96-97 -0.6498218774565849 2308 98-99 -0.9116126137423066 2308 100-101 -1.1891161291022527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 7.0 12 99.0 13 818.0 14 3361.0 15 8810.0 16 15329.0 17 21926.0 18 28167.0 19 34909.0 20 41704.0 21 46217.0 22 51661.0 23 59643.0 24 71475.0 25 90378.0 26 115990.0 27 150922.0 28 191988.0 29 247620.0 30 317171.0 31 410214.0 32 535299.0 33 717825.0 34 1025368.0 35 1598028.0 36 2583369.0 37 3485388.0 38 3255142.0 39 630058.0 40 109.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.48517836417031 12.820328449667523 13.299832514981086 37.394660671181086 2 23.538575382950437 19.050419657609815 30.31813053546344 27.09287442397631 3 26.442713746859457 19.108779483336004 22.674828083962236 31.7736786858423 4 28.660357874366376 23.62861091377736 20.51260351126791 27.198427700588358 5 29.421992954442132 26.08301228890409 22.24729088483731 22.247703871816466 6 25.711184227455437 30.403396150770746 22.127537368173762 21.757882253600055 7 21.60981689110391 21.114270637991815 36.470702227175245 20.80521024372903 8 22.56834060878728 23.353498746266833 28.957344481016733 25.120816163929145 9 22.430561798895038 22.006398756718585 30.983846173151463 24.579193271234917 10-11 25.27750024699798 29.18009059663593 23.290235494705982 22.2521736616601 12-13 24.381912568115055 24.75177417617834 26.619225052171373 24.247088203535235 14-15 24.475698734258444 25.39496327433867 26.76713792716752 23.36220006423536 16-17 25.00043363632811 24.94602419023578 26.011781565468446 24.041760607967664 18-19 24.772321866802805 24.95245407981895 26.24517639150403 24.030047661874217 20-21 24.817855269666204 25.045303083201947 25.889807449586204 24.24703419754565 22-23 24.936881293881854 25.44982382928516 25.58706893292742 24.026225943905565 24-25 24.649397880868506 25.507848499856568 25.77804681938078 24.064706799894147 26-27 24.883053206383256 25.50260356522129 25.659694281623445 23.954648946772014 28-29 24.736824047532895 25.796707477192793 25.43883202199378 24.02763645328053 30-31 24.55629252345234 25.43540479367396 25.569188065599423 24.439114617274278 32-33 24.474307159738533 25.484390456426766 25.59038963641512 24.45091274741958 34-35 24.996539645088465 25.34366160985209 25.207423537434178 24.452375207625266 36-37 25.18251804244793 25.20710390770074 25.312970217777586 24.29740783207374 38-39 25.09300391190959 25.33997340426292 25.308659447841897 24.25836323598559 40-41 24.773020767844454 25.80010985453646 25.049560025910168 24.377309351708924 42-43 24.660678779045295 25.821070532140077 25.408353582932076 24.109897105882556 44-45 24.809836968624744 25.606431668604003 25.588870191521124 23.994861171250133 46-47 24.87969690679505 25.67936453052392 25.675819149745255 23.76511941293577 48-49 24.747598901519922 25.51060948718069 25.966812162371834 23.774979448927553 50-51 24.67250256720577 25.546960010298047 25.676492553292473 24.104044869203708 52-53 24.62274667859727 25.709138650993886 25.509212284333465 24.158902386075376 54-55 24.71393186159599 25.452921231628828 25.444566187358213 24.388580719416964 56-57 24.28573107749256 25.75402368448557 25.610227971989318 24.350017266032552 58-59 24.57370372123506 25.455761311316255 25.611266793083036 24.359268174365646 60-61 24.5964878951928 25.339324397777624 25.772398428235093 24.291789278794486 62-63 24.51203283665929 25.72227215658535 25.66815179965912 24.097543207096233 64-65 24.792210327065415 25.609648318011725 25.68714375101023 23.910997603912627 66-67 24.76296930013638 25.843343237608245 25.372696922516337 24.02099053973904 68-69 24.95320381002726 25.3356805818923 25.66325867693585 24.047856931144587 70-71 24.66997416289922 25.664637418081647 25.465368023816005 24.200020395203126 72-73 24.44363823738428 25.343457444392097 25.526105701158176 24.68679861706545 74-75 24.582824379828573 25.434578256108477 25.645655901155063 24.336941462907895 76-77 24.60815001211958 25.318509854028164 25.70327711521606 24.370063018636195 78-79 24.488364727226866 25.498918450638048 25.724104366257183 24.2886124558779 80-81 24.39142715275022 25.428593121733627 25.864110129013955 24.31586959650219 82-83 24.1702090889539 25.576277265479785 25.459017554805758 24.79449609076056 84-85 24.16788365457896 25.39284433345331 25.619434404801577 24.81983760716615 86-87 24.207114240775855 25.641811945425996 25.13690359517873 25.014170218619427 88-89 24.181042055099454 25.814913849327738 25.35179660454813 24.65224749102468 90-91 24.107905237913858 25.430905848816902 25.814882081098574 24.64630683217067 92-93 24.147933206662813 25.521289637616633 25.597660460531312 24.733116695189242 94-95 24.566072992589426 25.495817871471466 25.215593498822507 24.7225156371166 96-97 24.70394393034625 25.266829298821175 25.193082531635596 24.836144239196976 98-99 24.57460593894337 25.3810233753807 24.90539262513267 25.138978060543256 100-101 24.600897325401018 25.689407106362257 24.909065032424245 24.800630535812484 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4417.0 1 2960.5 2 1606.0 3 2233.0 4 2672.0 5 2108.0 6 1488.0 7 1247.5 8 1063.5 9 922.5 10 845.0 11 838.0 12 815.5 13 850.0 14 960.0 15 1077.5 16 1277.0 17 1590.5 18 2070.5 19 2862.0 20 4089.0 21 6134.5 22 9268.0 23 14177.5 24 22249.5 25 32520.5 26 45727.5 27 63159.0 28 84414.0 29 108586.0 30 136749.5 31 167101.5 32 194342.5 33 223295.0 34 254258.5 35 284962.5 36 318511.0 37 351537.5 38 380631.0 39 405709.0 40 427933.0 41 448293.5 42 464649.0 43 476798.5 44 485689.0 45 499050.0 46 502993.0 47 487561.0 48 472296.0 49 459123.5 50 457773.5 51 480098.5 52 475991.0 53 461364.0 54 467548.5 55 449905.0 56 417047.0 57 418929.0 58 422693.5 59 408632.5 60 388467.0 61 371693.5 62 375401.0 63 384462.5 64 359141.5 65 288452.5 66 221479.0 67 181393.0 68 159194.5 69 132881.5 70 100747.0 71 75738.5 72 64831.5 73 55707.5 74 47050.0 75 40804.5 76 35160.0 77 31510.5 78 26868.0 79 20302.0 80 15008.5 81 11493.5 82 9748.5 83 7454.0 84 3956.0 85 2210.0 86 1380.5 87 945.0 88 638.5 89 442.0 90 304.0 91 210.5 92 170.5 93 118.0 94 68.0 95 38.0 96 17.5 97 8.0 98 3.5 99 3.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.062437277602540704 2 0.005889829687346619 3 3.9392604165640815E-4 4 5.0829166665342996E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 6.766632812323786E-4 32-33 0.0012326072916345676 34-35 1.5884114582919686E-5 36-37 0.001759959895787501 38-39 4.129869791559118E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0013088510416325821 48-49 9.975223958073561E-4 50-51 5.908890624846123E-4 52-53 0.0012739059895501587 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.1768229165839372E-6 64-65 1.9060937499503623E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5738995E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.6550124628946 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81189505514251 46.350539339621236 2 10.221923760148345 11.582464360519257 3 3.312145695340427 5.6294896694530365 4 1.4884050992518323 3.3730243779179365 5 0.8098362841584898 2.294064238595175 6 0.5221607148139205 1.7749813083249966 7 0.33251834153479093 1.3187181548656235 8 0.24994705751327034 1.132860292678253 9 0.17034367936025177 0.8685740949417345 >10 0.9664326368507404 10.027927212139693 >50 0.06955589507891133 2.6954937550271736 >100 0.036985168349281096 3.9193828805965225 >500 0.003739406899507709 1.5005423139451928 >1k 0.0034466958279795283 4.157775630453921 >5k 4.843037013625949E-4 1.8326697219040762 >10k+ 1.802060284139888E-4 1.5414926490163892 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC 32482 0.20637912395295885 No Hit GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC 23885 0.15175683072521468 No Hit GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG 21852 0.13883986874638438 No Hit CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG 16723 0.10625201926806635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.353645833167874E-6 0.0 0.0 6.353645833167874E-6 2.5414583332671495E-5 2 1.906093749950362E-5 5.082916666534299E-5 0.0 3.812187499900724E-5 3.176822916583937E-5 3 3.176822916583937E-5 6.989010416484661E-5 0.0 3.812187499900724E-5 3.176822916583937E-5 4 5.082916666534299E-5 6.989010416484661E-5 0.0 6.353645833167874E-5 3.176822916583937E-5 5 5.7182812498510864E-5 6.989010416484661E-5 1.2707291666335747E-5 6.353645833167874E-5 3.176822916583937E-5 6 1.5884114582919684E-4 7.624374999801448E-5 1.2707291666335747E-5 6.353645833167874E-5 3.176822916583937E-5 7 1.8425572916186834E-4 8.895104166435023E-5 1.2707291666335747E-5 6.353645833167874E-5 3.176822916583937E-5 8 2.0967031249453984E-4 9.530468749751811E-5 1.2707291666335747E-5 7.624374999801448E-5 3.176822916583937E-5 9 2.1602395832770772E-4 1.0165833333068598E-4 1.2707291666335747E-5 7.624374999801448E-5 3.176822916583937E-5 10-11 2.827372395759704E-4 1.1436562499702173E-4 1.2707291666335747E-5 8.577421874776629E-5 3.176822916583937E-5 12-13 3.3992005207448124E-4 1.3978020832969322E-4 1.2707291666335747E-5 9.530468749751811E-5 3.176822916583937E-5 14-15 4.0663333332274397E-4 1.7472526041211654E-4 1.2707291666335747E-5 1.0165833333068598E-4 3.176822916583937E-5 16-17 5.0829166665343E-4 2.0013984374478802E-4 1.2707291666335747E-5 1.0165833333068598E-4 3.176822916583937E-5 18-19 6.925473958152982E-4 2.255544270774595E-4 1.2707291666335747E-5 1.1118880208043779E-4 3.176822916583937E-5 20-21 8.926872395600863E-4 2.319080729106274E-4 1.2707291666335747E-5 1.1436562499702173E-4 3.176822916583937E-5 22-23 0.0013279119791320855 2.4143854166037922E-4 1.2707291666335747E-5 1.270729166633575E-4 3.176822916583937E-5 24-25 0.001979160677031793 2.541458333267149E-4 1.906093749950362E-5 1.3978020832969322E-4 3.176822916583937E-5 26-27 0.00303386588533766 2.668531249930507E-4 1.906093749950362E-5 1.5248749999602896E-4 3.176822916583937E-5 28-29 0.005032087499868956 2.8591406249255433E-4 1.906093749950362E-5 1.556643229126129E-4 4.1298697915591174E-5 30-31 0.007239979426894793 3.0815182290864183E-4 1.906093749950362E-5 1.6519479166236472E-4 4.4475520832175115E-5 32-33 0.009876742447659459 3.208591145749776E-4 1.906093749950362E-5 1.7472526041211654E-4 4.4475520832175115E-5 34-35 0.012802596353833266 3.2403593749156156E-4 1.906093749950362E-5 1.8743255207845228E-4 4.4475520832175115E-5 36-37 0.015795163541255333 3.494505208242331E-4 1.906093749950362E-5 1.9060937499503622E-4 5.082916666534299E-5 38-39 0.018225433072442045 3.685114583237367E-4 1.906093749950362E-5 1.9696302082820407E-4 5.082916666534299E-5 40-41 0.02099562265570324 3.780419270734885E-4 1.906093749950362E-5 2.1284713541112378E-4 5.082916666534299E-5 42-43 0.024099378645205744 3.9074921873982424E-4 1.906093749950362E-5 2.319080729106274E-4 5.082916666534299E-5 44-45 0.027091945832627813 4.066333333227439E-4 1.906093749950362E-5 2.573226562432989E-4 5.082916666534299E-5 46-47 0.03085648098877978 4.2569427082224756E-4 1.906093749950362E-5 2.7320677082621857E-4 5.7182812498510864E-5 48-49 0.03541839869699431 4.479320312383351E-4 1.906093749950362E-5 2.8591406249255433E-4 5.7182812498510864E-5 50-51 0.04021222447811947 4.5428567707150294E-4 2.5414583332671495E-5 2.986213541588901E-4 5.7182812498510864E-5 52-53 0.04593685937380373 4.7016979165442267E-4 2.5414583332671495E-5 3.01798177075474E-4 6.0359635415094804E-5 54-55 0.05576594947771443 4.892307291539263E-4 2.5414583332671495E-5 3.049749999920579E-4 6.353645833167874E-5 56-57 0.06219901588379689 5.019380208202621E-4 2.5414583332671495E-5 3.049749999920579E-4 6.671328124826267E-5 58-59 0.06810472968572645 5.146453124865978E-4 2.5414583332671495E-5 3.1450546874180977E-4 7.942057291459842E-5 60-61 0.07381348046682779 5.209989583197656E-4 2.5414583332671495E-5 3.4309687499106515E-4 8.259739583118236E-5 62-63 0.07983038307083776 5.400598958192693E-4 2.5414583332671495E-5 3.558041666574009E-4 8.259739583118236E-5 64-65 0.08625391900817048 5.654744791519407E-4 2.8591406249255432E-5 3.780419270734885E-4 8.895104166435023E-5 66-67 0.09393229999755384 5.813585937348605E-4 3.176822916583937E-5 3.971028645729921E-4 8.895104166435023E-5 68-69 0.10194742421609512 5.877122395680284E-4 3.176822916583937E-5 4.1616380207249576E-4 8.895104166435023E-5 70-71 0.11054708385128784 5.972427083177801E-4 3.176822916583937E-5 4.225174479056636E-4 8.895104166435023E-5 72-73 0.1272095200487706 6.417182291499552E-4 4.1298697915591174E-5 4.574624999880869E-4 8.895104166435023E-5 74-75 0.1385253632776426 6.734864583157947E-4 4.4475520832175115E-5 4.7334661457100664E-4 9.212786458093417E-5 76-77 0.15218887864187008 6.798401041489625E-4 4.4475520832175115E-5 4.955843749870942E-4 9.848151041410205E-5 78-79 0.17130064530803907 6.925473958152982E-4 4.4475520832175115E-5 5.0829166665343E-4 1.0165833333068598E-4 80-81 0.19093023410961119 7.147851562313858E-4 4.4475520832175115E-5 5.0829166665343E-4 1.0165833333068598E-4 82-83 0.21532505728605925 7.211388020645537E-4 4.4475520832175115E-5 5.146453124865978E-4 1.0165833333068598E-4 84-85 0.2436114885353226 7.306692708143054E-4 4.4475520832175115E-5 5.273526041529335E-4 1.0165833333068598E-4 86-87 0.2817428940030796 7.497302083138091E-4 4.4475520832175115E-5 5.781817708182765E-4 1.0165833333068598E-4 88-89 0.33090105181429946 7.592606770635609E-4 4.4475520832175115E-5 5.908890624846123E-4 1.0483515624726992E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACGC 5160 0.0 46.24087 1 CGACGCT 7430 0.0 33.053467 2 GACGCTC 9550 0.0 25.963163 3 GGGCGAT 5795 0.0 25.18007 1 GCGATCT 6215 0.0 23.310371 3 ACGGGGT 6465 0.0 22.998056 2 CGATCTG 6525 0.0 22.712408 4 GGCGATC 6610 0.0 22.709152 2 ACGCTCA 11365 0.0 22.527262 4 ACCGCGT 5270 0.0 21.541452 8 AGACCGC 5395 0.0 21.482563 6 CCGCGTT 5630 0.0 20.417131 9 TCGCAGC 13940 0.0 20.205969 92-93 GACCGCG 5615 0.0 20.133299 7 GGCGCGT 6695 0.0 19.013931 5 GTCTCGC 9375 0.0 18.543749 7 GACGGGG 7435 0.0 18.539118 1 CTCGCTA 9470 0.0 18.056778 9 AAGTGCG 15635 0.0 17.802961 40-41 CAGACCG 6530 0.0 17.530392 5 >>END_MODULE