FastQCFastQC Report
Thu 26 May 2016
SRR1562286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19134975
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25400.010.4728841
CTCCGAT40050.010.4373961
CGTATCG19650.09.4277563
GTCTAAC33100.08.6106371
CTCGGAT61550.08.4893921
CTCCAAT133150.08.3480821
CCCGGAT66450.08.1493291
TCCGATT37650.08.0747112
CTGGAAT222900.08.0342111
TCGGATT31650.07.80443762
CTCAGAT141350.07.796581
CTTGGAT148150.07.5349121
GTATCGA22850.07.48380764
CTGGGAT225600.07.4327171
CTCGAAT44650.07.34073641
TCGTAAT21550.07.274092
CTCGAGT38550.07.27008721
CTCGATT34850.07.2241221
CCCGGTT36450.07.1676561
CCGCAAT27250.07.14709141
Produced by FastQC (version 0.11.5)