FastQCFastQC Report
Thu 26 May 2016
SRR1562286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19134975
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT44700.08.6221081
CCCGGAT65800.08.532811
CTCGGAT64700.08.5307981
GTCCGTT20800.08.4639751
TCGGATT31850.07.75460622
CTCGGTA25750.07.57604551
ACGCGAT13306.9412636E-97.4995212
CCGGGAT60100.07.28365371
CTGGAAT215150.07.2538481
CTCAGAT145500.07.227121
CTCCAAT133100.07.22119861
CTGGGAT229150.07.1221351
TCGAATA25350.07.119862
CGATTAG20100.07.08909944
CTTGGAT150800.07.0362211
CTCGCAT51400.07.03536131
GTCTAAC32650.06.99510151
CTCGGTT34700.06.99321171
CTCCGAT34750.06.9831491
CTGCAAT136050.06.9596991
Produced by FastQC (version 0.11.5)