FastQCFastQC Report
Thu 26 May 2016
SRR1562285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14799567
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC27500.011.2276371
TGCGCGA22250.011.10110959
CTCCGAT29200.09.9232671
CCCGTAT21150.09.8821171
CTCGGAT46400.09.4184081
CTCGCAT37500.09.2469661
TCCGATC17600.09.17643452
GTGCGCG35850.09.009758
CGGGGTT49250.08.8733841
GTTCGAT20900.08.8639241
TCGGATT25950.08.4203122
CCGTAAT15300.08.3826261
ACGTGCG33600.08.3407636
CTCGTAT19650.08.2190961
CTCCAAT105400.08.0220821
CCCGGTT27500.07.77297971
CGCATTC28400.07.6938083
CGGGGAT48900.07.67407271
CCGGCAT34800.07.6439261
TAACACG33150.07.5945152
Produced by FastQC (version 0.11.5)