FastQCFastQC Report
Thu 26 May 2016
SRR1562285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14799567
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA23250.014.5042049
CGCGTAT3000.005805543611.10224151
GTCTAAC27500.010.5543381
TAACACG30850.09.5455482
CCCGTAT23550.09.2939611
TACCGTA14200.09.0309599
CTCGGAT46300.08.9407131
CTAACGC7552.8935137E-68.8229731
GTGCGCG35000.08.8207148
ACGTGCG34450.08.6859176
CCCGGTT26650.08.5699421
CGCGTTT9459.05693E-88.5595511
CGTATCG16750.08.2233273
CTCGCGT12351.5879777E-98.0907011
GTATCGA20400.07.9161334
CGCATAT9651.1338143E-67.8890831
CGCCAAT25500.07.83687641
CGATTAG13952.6921043E-107.8310134
CTCGGTT30650.07.7619961
CGGCAAT23350.07.74338051
Produced by FastQC (version 0.11.5)