##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1562284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19280661 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825845389844257 31.0 31.0 34.0 30.0 34.0 2 32.04802931808199 33.0 31.0 34.0 30.0 34.0 3 32.27895283258183 33.0 31.0 34.0 30.0 34.0 4 36.051678674294415 37.0 35.0 37.0 35.0 37.0 5 35.993742278856516 37.0 35.0 37.0 35.0 37.0 6 35.978618367907615 37.0 35.0 37.0 35.0 37.0 7 35.95767230179505 37.0 35.0 37.0 35.0 37.0 8 35.95600664313324 37.0 35.0 37.0 35.0 37.0 9 37.70957691751336 39.0 37.0 39.0 35.0 39.0 10-11 37.6941875851663 39.0 37.0 39.0 35.0 39.0 12-13 37.62091561591171 39.0 37.0 39.0 35.0 39.0 14-15 38.90535895008993 40.0 38.0 41.0 36.0 41.0 16-17 38.836279964675484 40.0 38.0 41.0 35.0 41.0 18-19 38.81942325525043 40.0 38.0 41.0 35.0 41.0 20-21 38.787699057620486 40.0 38.0 41.0 35.0 41.0 22-23 38.6644052556082 40.0 38.0 41.0 34.5 41.0 24-25 38.57438873594634 40.0 38.0 41.0 34.0 41.0 26-27 38.38039198967297 40.0 38.0 41.0 34.0 41.0 28-29 38.21581179192975 40.0 38.0 41.0 34.0 41.0 30-31 38.09180406211178 40.0 38.0 41.0 33.5 41.0 32-33 37.91618448662108 40.0 37.0 41.0 33.0 41.0 34-35 37.757558130398124 39.5 37.0 41.0 33.0 41.0 36-37 37.741143262671336 39.5 37.0 40.5 33.0 41.0 38-39 37.87125960048776 40.0 37.0 41.0 33.0 41.0 40-41 37.833859352643564 40.0 37.0 41.0 33.0 41.0 42-43 37.67440662433721 39.5 37.0 41.0 33.0 41.0 44-45 37.50117400020673 39.0 36.0 41.0 32.0 41.0 46-47 37.36279036284078 39.0 36.0 41.0 32.0 41.0 48-49 37.17118046419674 39.0 35.0 40.0 31.5 41.0 50-51 36.95038528502731 39.0 35.0 40.0 31.0 41.0 52-53 36.70645731492297 38.5 35.0 40.0 31.0 41.0 54-55 36.42329578327216 38.0 35.0 40.0 30.5 41.0 56-57 36.135131881629995 38.0 34.5 40.0 30.0 41.0 58-59 35.81920334577741 37.5 34.0 40.0 30.0 41.0 60-61 35.50767886018016 37.0 34.0 40.0 29.0 41.0 62-63 35.25055191313203 36.5 34.0 39.5 29.0 41.0 64-65 34.993648661734156 36.0 34.0 39.0 29.0 41.0 66-67 34.698952359569006 35.5 33.5 39.0 29.0 40.5 68-69 34.35263197148687 35.0 33.0 38.5 28.0 40.0 70-71 34.05076075970632 35.0 33.0 37.5 28.0 40.0 72-73 33.6986276300382 35.0 33.0 37.0 28.0 39.0 74-75 33.36333295315964 35.0 33.0 36.5 27.0 39.0 76-77 32.61479297831127 34.0 31.5 36.0 26.0 38.5 78-79 32.7575598419577 35.0 32.0 36.0 27.0 37.5 80-81 32.620133770310055 35.0 32.5 35.0 27.0 37.0 82-83 32.33475400558103 35.0 32.0 35.0 26.0 37.0 84-85 32.0728933515298 34.5 32.0 35.0 26.0 36.0 86-87 31.855104111835168 34.0 32.0 35.0 25.5 36.0 88-89 31.681513486493017 34.0 32.0 35.0 25.0 36.0 90-91 31.50729248857184 34.0 32.0 35.0 25.0 35.0 92-93 31.268568074507407 34.0 31.5 35.0 24.5 35.0 94-95 30.99057174440233 34.0 31.0 35.0 24.0 35.0 96-97 30.768507651267765 34.0 31.0 35.0 23.5 35.0 98-99 30.47383015032524 34.0 31.0 35.0 21.5 35.0 100-101 29.985307116804762 34.0 30.5 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3881948358264715 1101 2 0.44671655218745343 1101 3 0.404636960484801 1101 4 0.24144330002752667 1101 5 0.17678786741718255 1101 6 0.21221180446440258 1101 7 0.23467155961404274 1101 8 0.26577148374921933 1101 9 0.2756343268922379 1101 10-11 0.24440107077996487 1101 12-13 0.28688837177393367 1101 14-15 0.3479663223820211 1101 16-17 0.3893759739165077 1101 18-19 0.4001871066874685 1101 20-21 0.32405027981613443 1101 22-23 0.3445292186873772 1101 24-25 0.3740222422754442 1101 26-27 0.35993046684754404 1101 28-29 0.3367715543155043 1101 30-31 0.20334613674108937 1101 32-33 0.25912397730004244 1101 34-35 0.19442515112164216 1101 36-37 0.2212128487343037 1101 38-39 0.3213933898077457 1101 40-41 0.3048957579838216 1101 42-43 0.31914160178747153 1101 44-45 0.3239361385771815 1101 46-47 0.290744452308644 1101 48-49 0.3145326756518827 1101 50-51 0.24705633328470356 1101 52-53 0.3027271168335872 1101 54-55 0.2525138006384964 1101 56-57 0.18969163013625945 1101 58-59 0.15476228201954 1101 60-61 0.17071145726085746 1101 62-63 0.21002082613728845 1101 64-65 0.1767861824598782 1101 66-67 0.11187282759392758 1101 68-69 0.07177631247200367 1101 70-71 -0.004813498228372737 1101 72-73 -0.07684392157967324 1101 74-75 -0.044936786811703655 1101 76-77 -0.1657400525717847 1101 78-79 -0.12455461371272492 1101 80-81 -0.17732796661952221 1101 82-83 -0.18801906012166825 1101 84-85 -0.25910350636328516 1101 86-87 -0.29091048390923646 1101 88-89 -0.2532317003143483 1101 90-91 -0.3067870102786614 1101 92-93 -0.2954598314972543 1101 94-95 -0.42835345752753184 1101 96-97 -0.6278128151623221 1101 98-99 -0.7920289398198932 1101 100-101 -0.743516076277519 1102 1 0.27596270363507003 1102 2 0.33075071699901315 1102 3 0.3506591554096161 1102 4 0.20513597578427323 1102 5 0.19371926586438093 1102 6 0.1479321783279559 1102 7 0.17439800799306937 1102 8 0.1605520083782963 1102 9 0.1995608774397084 1102 10-11 0.1909891686466878 1102 12-13 0.1766482048436515 1102 14-15 0.22658299118155867 1102 16-17 0.2672267168147471 1102 18-19 0.25179671150094407 1102 20-21 0.2472154071679995 1102 22-23 0.2423131725224721 1102 24-25 0.24236028838717516 1102 26-27 0.26929070426906776 1102 28-29 0.30569630980572526 1102 30-31 0.27181344384433714 1102 32-33 0.2617045277323129 1102 34-35 0.22693175796860743 1102 36-37 0.2313931951510284 1102 38-39 0.30573841402876667 1102 40-41 0.3143831556697805 1102 42-43 0.3621299672137326 1102 44-45 0.3587529668230971 1102 46-47 0.2614036713153425 1102 48-49 0.280160356037058 1102 50-51 0.24792897451563078 1102 52-53 0.3173398969984831 1102 54-55 0.29230983811289946 1102 56-57 0.31370322197245315 1102 58-59 0.31598832258438136 1102 60-61 0.2535043895955127 1102 62-63 0.3112708973330598 1102 64-65 0.3290877097406977 1102 66-67 0.3754944280812609 1102 68-69 0.39006010118111334 1102 70-71 0.3366419710567783 1102 72-73 0.2692223303721377 1102 74-75 0.2676302607252268 1102 76-77 0.24802536136262887 1102 78-79 0.33371775435136186 1102 80-81 0.22558109254313763 1102 82-83 0.2990244163334381 1102 84-85 0.1193442406812153 1102 86-87 0.19101163478690708 1102 88-89 0.2702362048735303 1102 90-91 0.2761329839116051 1102 92-93 0.2517364148127186 1102 94-95 0.15027881007441124 1102 96-97 0.10228928258438685 1102 98-99 0.05033203522595997 1102 100-101 0.10487847818798812 1103 1 0.23883977186512695 1103 2 0.3199965123799089 1103 3 0.297105163198502 1103 4 0.1776615367399188 1103 5 0.11758917333175845 1103 6 0.10618406042473083 1103 7 0.09820369840193166 1103 8 0.13383913103098166 1103 9 0.15575726355440622 1103 10-11 0.15487958310210814 1103 12-13 0.17118159036481728 1103 14-15 0.19212478856432824 1103 16-17 0.2184990656615753 1103 18-19 0.1979277971454394 1103 20-21 0.19715088001397163 1103 22-23 0.2527459116718802 1103 24-25 0.23957652876367774 1103 26-27 0.19766097512496827 1103 28-29 0.2471751327074898 1103 30-31 0.16904709236246163 1103 32-33 0.11636110080260664 1103 34-35 0.1343329469963379 1103 36-37 0.15922854536415088 1103 38-39 0.13501809949963928 1103 40-41 0.1310626981221219 1103 42-43 0.09510296181461086 1103 44-45 0.11066739477695364 1103 46-47 2.9031252862665724E-4 1103 48-49 0.007939312281131095 1103 50-51 -0.02696166317134896 1103 52-53 -0.05484117916763864 1103 54-55 -0.123528854942208 1103 56-57 -0.0807439628893647 1103 58-59 -0.1327687308945542 1103 60-61 -0.24726144645801185 1103 62-63 -0.1958348739375353 1103 64-65 -0.408531189094532 1103 66-67 -0.35533952987745465 1103 68-69 -0.32768484695063904 1103 70-71 -0.36267813741015686 1103 72-73 -0.5021775525705365 1103 74-75 -0.44527327043527976 1103 76-77 -0.5169243275638209 1103 78-79 -0.47124073886876516 1103 80-81 -0.44762249317060565 1103 82-83 -0.33480433767688567 1103 84-85 -0.41960015892134805 1103 86-87 -0.4503374733033709 1103 88-89 -0.34800983031042776 1103 90-91 -0.2828536530558523 1103 92-93 -0.23626795506558196 1103 94-95 -0.2576244936620391 1103 96-97 -0.30644846583668794 1103 98-99 -0.30720045723254685 1103 100-101 -0.17369738971073545 1104 1 0.12838303070190804 1104 2 0.19711895370250687 1104 3 0.17750274474924055 1104 4 0.09562656306728456 1104 5 0.05207505488518649 1104 6 -0.03166218226398598 1104 7 -0.03561819818900602 1104 8 0.01020036514206879 1104 9 -0.060696890121832325 1104 10-11 0.037687576247911636 1104 12-13 0.08252146396941384 1104 14-15 0.0939328620604698 1104 16-17 0.10374157637998849 1104 18-19 0.07813036929181294 1104 20-21 0.06443009122100563 1104 22-23 -5.913005509228242E-6 1104 24-25 -0.1261123797586805 1104 26-27 -0.015398692229176447 1104 28-29 0.08735441787378306 1104 30-31 -0.02603069513877898 1104 32-33 0.027868191353242366 1104 34-35 -0.01331260083832575 1104 36-37 -0.06469307636687915 1104 38-39 -0.3536209727546762 1104 40-41 -0.18603900950368057 1104 42-43 -0.13459243546244437 1104 44-45 -0.3746639956092537 1104 46-47 -0.1775344897467832 1104 48-49 -0.2387289055631001 1104 50-51 -0.08087351747913374 1104 52-53 -0.26843716119190475 1104 54-55 -0.11623043861190752 1104 56-57 -0.17475370887184027 1104 58-59 -0.30917558440249593 1104 60-61 -0.2008800481375843 1104 62-63 0.023944984336736752 1104 64-65 -0.09266787959895595 1104 66-67 -0.2275545273839441 1104 68-69 -0.11931661501554913 1104 70-71 0.09012053130867059 1104 72-73 -2.3748328015926745E-4 1104 74-75 -0.04776510119947375 1104 76-77 -0.085681183782647 1104 78-79 0.08896000649422575 1104 80-81 0.031130426882363338 1104 82-83 0.09337560146186519 1104 84-85 0.09225511352097726 1104 86-87 0.1260367583222397 1104 88-89 0.2695030468478272 1104 90-91 0.16094349074558423 1104 92-93 0.2918656579598071 1104 94-95 0.2438509357847316 1104 96-97 0.1495334022687267 1104 98-99 0.19704177522573119 1104 100-101 0.3181792958916674 1105 1 0.089656246913421 1105 2 0.13860846290702966 1105 3 0.1343781738464216 1105 4 0.056734192071509426 1105 5 0.03424974979871109 1105 6 -0.03646902697163057 1105 7 -0.030105569499824014 1105 8 -0.05692033605021862 1105 9 -0.14484270711798075 1105 10-11 -0.02059159640205621 1105 12-13 -0.02335486093836181 1105 14-15 -0.034567770128703046 1105 16-17 0.004246524741859048 1105 18-19 -0.046053490626064786 1105 20-21 -0.0038263564806015893 1105 22-23 0.006404369128418352 1105 24-25 0.015163214595020236 1105 26-27 0.04841013916853143 1105 28-29 0.09513784331264219 1105 30-31 0.045534858363581066 1105 32-33 0.06805782807837346 1105 34-35 0.08364313034961413 1105 36-37 0.02417965551533996 1105 38-39 0.06418690244564829 1105 40-41 0.08421604207549649 1105 42-43 -0.02329502394668026 1105 44-45 0.06551760903358428 1105 46-47 0.11180074260200712 1105 48-49 0.1262180116614573 1105 50-51 0.10059444667322737 1105 52-53 0.1605382320378297 1105 54-55 0.15411241847061774 1105 56-57 0.2044653677566899 1105 58-59 0.2613268608429351 1105 60-61 0.2162153153858739 1105 62-63 0.2912118125669636 1105 64-65 0.288374097757945 1105 66-67 0.32225734512768867 1105 68-69 0.3397323673276915 1105 70-71 0.4081675501891837 1105 72-73 0.3110568628531709 1105 74-75 0.38895650882208344 1105 76-77 0.3887821957694513 1105 78-79 0.45773200332993724 1105 80-81 0.4295150830600747 1105 82-83 0.5309091308038987 1105 84-85 0.486767103744171 1105 86-87 0.5565271631012401 1105 88-89 0.6256670382145266 1105 90-91 0.648934268431848 1105 92-93 0.7035618123937546 1105 94-95 0.6743282466595062 1105 96-97 0.5811908449017409 1105 98-99 0.563339711436516 1105 100-101 0.7772153096984447 1106 1 -0.11039821552618179 1106 2 -0.038680998315854254 1106 3 -0.05863394274205547 1106 4 -0.08002089208700625 1106 5 -0.1215934643802754 1106 6 -0.1497968209356415 1106 7 -0.22868440254524103 1106 8 -0.20703711906214295 1106 9 -0.2282217000039708 1106 10-11 -0.26646577786253545 1106 12-13 -0.23435267712545027 1106 14-15 -0.31972446795822407 1106 16-17 -0.3315993077549706 1106 18-19 -0.31134982854634075 1106 20-21 -0.3342332778224204 1106 22-23 -0.313578751417424 1106 24-25 -0.28497928092394886 1106 26-27 -0.35503897443508237 1106 28-29 -0.2903727129560778 1106 30-31 -0.2950209831889339 1106 32-33 -0.3514949337589641 1106 34-35 -0.3209151095561751 1106 36-37 -0.33508632925870074 1106 38-39 -0.34387317398986994 1106 40-41 -0.3500650047819036 1106 42-43 -0.4201793903332387 1106 44-45 -0.4295358055715113 1106 46-47 -0.39534281447421193 1106 48-49 -0.4323292660626379 1106 50-51 -0.36067101691526204 1106 52-53 -0.36308741299564673 1106 54-55 -0.3613836477606611 1106 56-57 -0.49346811089996123 1106 58-59 -0.4253128359864107 1106 60-61 -0.3419288169938426 1106 62-63 -0.4266855916697523 1106 64-65 -0.528361972125559 1106 66-67 -0.28103616971799283 1106 68-69 -0.30288597314055465 1106 70-71 -0.4377651191870484 1106 72-73 -0.35919016541769366 1106 74-75 -0.19781613530978603 1106 76-77 -0.34417847204816354 1106 78-79 -0.30234113856888456 1106 80-81 -0.2660287469650484 1106 82-83 -0.10013613831397805 1106 84-85 -0.2179914285597171 1106 86-87 -0.3076514007057689 1106 88-89 -0.22199945424529588 1106 90-91 -0.2520838410413724 1106 92-93 -0.23231389864531593 1106 94-95 -0.3276327915959847 1106 96-97 -0.4288747360961267 1106 98-99 -0.38925191749933674 1106 100-101 -0.15544783718383215 1107 1 -0.2713700414252642 1107 2 -0.29006187614023915 1107 3 -0.3000837268853509 1107 4 -0.24360307090872624 1107 5 -0.28837710253913684 1107 6 -0.3347501270784434 1107 7 -0.373513847886052 1107 8 -0.38606624273728585 1107 9 -0.44020341696196397 1107 10-11 -0.4500911951816491 1107 12-13 -0.5521691657257435 1107 14-15 -0.7259877581517244 1107 16-17 -0.728579684735422 1107 18-19 -0.643038627286387 1107 20-21 -0.608504072738242 1107 22-23 -0.6067722838038492 1107 24-25 -0.6060279264028878 1107 26-27 -0.6088547541948444 1107 28-29 -0.7078987287233502 1107 30-31 -0.6459604436030872 1107 32-33 -0.5791183018359689 1107 34-35 -0.602385914781209 1107 36-37 -0.6310560884450638 1107 38-39 -0.6543935699319334 1107 40-41 -0.6725806884754135 1107 42-43 -0.8025142786214516 1107 44-45 -0.6283110421404814 1107 46-47 -0.8576010747143457 1107 48-49 -0.6471516306660519 1107 50-51 -0.6192441956967372 1107 52-53 -0.5594627695861902 1107 54-55 -0.5807205359296788 1107 56-57 -0.39637006366554317 1107 58-59 -0.4565159066825899 1107 60-61 -0.3570953231271048 1107 62-63 -0.5925608890051564 1107 64-65 -0.5188942347554004 1107 66-67 -0.29526843099000644 1107 68-69 -0.2327924679170721 1107 70-71 -0.1961241645210947 1107 72-73 -0.3092166366850293 1107 74-75 -0.18987817885663105 1107 76-77 -0.35038990947116844 1107 78-79 -0.2468478606212372 1107 80-81 -0.21193982808173928 1107 82-83 -0.2421616509555733 1107 84-85 -0.3287461735952313 1107 86-87 -0.20560175087163302 1107 88-89 -0.05654298838497951 1107 90-91 -0.12064463236117362 1107 92-93 -0.12483585433786004 1107 94-95 -0.1527924129514524 1107 96-97 -0.2777682140554987 1107 98-99 -0.3217029105213456 1107 100-101 -0.14688652261054003 1108 1 -0.43937794760499926 1108 2 -0.44167411947119106 1108 3 -0.4271668033532201 1108 4 -0.3729106880684654 1108 5 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0.8263832853046402 2305 1 -0.39031227951163316 2305 2 -0.2637847389348593 2305 3 -0.06501409841284556 2305 4 -0.1253171810864444 2305 5 -0.057308885219327976 2305 6 -0.013386498998990248 2305 7 -0.012540730521934051 2305 8 -0.025338664961807922 2305 9 0.03452356853075145 2305 10-11 0.08593088395893744 2305 12-13 0.06634737643185673 2305 14-15 0.06858372942190272 2305 16-17 0.06697794089014053 2305 18-19 0.07132115826077268 2305 20-21 0.007976177096097103 2305 22-23 0.0036230616285095607 2305 24-25 -5.479786012401178E-4 2305 26-27 0.03411189047852048 2305 28-29 0.0502866117364249 2305 30-31 -0.07741724604826317 2305 32-33 0.001858120731689894 2305 34-35 0.04744586156721908 2305 36-37 0.04597623100202952 2305 38-39 0.0036896164234931916 2305 40-41 -0.00215910410248199 2305 42-43 0.11130757707139338 2305 44-45 0.14992139077393318 2305 46-47 0.2027711573461275 2305 48-49 0.17114097040607845 2305 50-51 0.18622676006024363 2305 52-53 0.2118510869399799 2305 54-55 0.2593219368574893 2305 56-57 0.2912461084035911 2305 58-59 0.11942251077734767 2305 60-61 0.24343412752725868 2305 62-63 0.28916597491384266 2305 64-65 0.24713037480896816 2305 66-67 0.14276092465445345 2305 68-69 0.2770136141864512 2305 70-71 0.3687058645636583 2305 72-73 0.38085039863626236 2305 74-75 0.3642754619854145 2305 76-77 0.44257142299944974 2305 78-79 0.41508331390082986 2305 80-81 0.41383052052666613 2305 82-83 0.39697571742339477 2305 84-85 0.4902815388913666 2305 86-87 0.5122203157167853 2305 88-89 0.427406704130636 2305 90-91 0.47172720569421855 2305 92-93 0.4585809969570853 2305 94-95 0.4486344903674997 2305 96-97 0.5531320641552497 2305 98-99 0.6446852085111274 2305 100-101 0.7155592913205133 2306 1 -1.180708688950638 2306 2 -0.7164348466021337 2306 3 -0.3699527770694644 2306 4 -0.3371693436884229 2306 5 -0.2854596268283558 2306 6 -0.18817064102000813 2306 7 -0.15778578912399865 2306 8 -0.12789586499422256 2306 9 -0.1911105160828086 2306 10-11 -0.22376762707370546 2306 12-13 -0.1911022931341293 2306 14-15 -0.24465438914701565 2306 16-17 -0.20208897012253146 2306 18-19 -0.19857159542088 2306 20-21 -0.1985416497072734 2306 22-23 -0.22459151291825208 2306 24-25 -0.19076995149575993 2306 26-27 -0.16848056940344236 2306 28-29 -0.18442148834827066 2306 30-31 -0.1494999312421399 2306 32-33 -0.1298169293410396 2306 34-35 -0.16401389620408935 2306 36-37 -0.09903944800149134 2306 38-39 -0.18387245288166554 2306 40-41 -0.14908731381979834 2306 42-43 -0.18867581629769603 2306 44-45 -0.1421432041438564 2306 46-47 -0.13223532498923163 2306 48-49 -0.12165201247827184 2306 50-51 -0.13277990841741882 2306 52-53 -0.22402890953456023 2306 54-55 -0.2684392926099832 2306 56-57 -0.05348654212166082 2306 58-59 -0.030657330002895833 2306 60-61 -0.06382917744320338 2306 62-63 -0.02997671396307311 2306 64-65 -0.04144083100619156 2306 66-67 -0.09266195245361075 2306 68-69 -0.08477182162483388 2306 70-71 -0.11745132613022236 2306 72-73 -0.06368872336351217 2306 74-75 -0.07399374440838358 2306 76-77 -0.03752063946507889 2306 78-79 -0.03796071215261776 2306 80-81 0.04092465375160259 2306 82-83 -0.025487896696454015 2306 84-85 0.11364266582113203 2306 86-87 -0.0012898483059622379 2306 88-89 3.534584350965986E-4 2306 90-91 -0.01160674543666218 2306 92-93 -0.18311163864046875 2306 94-95 3.920512697206391E-4 2306 96-97 0.1109513409494518 2306 98-99 0.06802565832911966 2306 100-101 0.10927854631509248 2307 1 -0.8801331849468639 2307 2 -1.2827839454186964 2307 3 -0.8238582236686547 2307 4 -0.4578308860936886 2307 5 -0.41552587818210895 2307 6 -0.38304777598519735 2307 7 -0.3290397897389994 2307 8 -0.34097521451111135 2307 9 -0.406481552628307 2307 10-11 -0.35207866886096184 2307 12-13 -0.5729375469128613 2307 14-15 -0.6219213342329795 2307 16-17 -0.5585577331012672 2307 18-19 -0.5086770152173301 2307 20-21 -0.44820129861476943 2307 22-23 -0.4881195999449872 2307 24-25 -0.45325239213021007 2307 26-27 -0.4478545506074809 2307 28-29 -0.39408219297099834 2307 30-31 -0.4248962296651655 2307 32-33 -0.4200930730121186 2307 34-35 -0.45263633479225973 2307 36-37 -0.36326254737304 2307 38-39 -0.5381965193188094 2307 40-41 -0.6202486698018319 2307 42-43 -0.4256235242417503 2307 44-45 -0.42710658770641174 2307 46-47 -0.46507747082419115 2307 48-49 -0.43461898732272886 2307 50-51 -0.3818468063281486 2307 52-53 -0.31760568799624167 2307 54-55 -0.2820352042062382 2307 56-57 -0.20767613388748174 2307 58-59 -0.2616513895996846 2307 60-61 -0.2958823731023372 2307 62-63 -0.3777108569250629 2307 64-65 -0.37236756596232823 2307 66-67 -0.24655182590312563 2307 68-69 -0.23310374961730673 2307 70-71 -0.1268671506952046 2307 72-73 -0.05883821156957225 2307 74-75 -0.005480615258065313 2307 76-77 -0.028294852605135645 2307 78-79 -0.049551806045606384 2307 80-81 -0.06183006811094316 2307 82-83 -0.10122964663731437 2307 84-85 -0.009914082776539601 2307 86-87 -0.056042644253921026 2307 88-89 -0.05346745260324326 2307 90-91 -0.02398559689072144 2307 92-93 -0.20309497817315503 2307 94-95 -0.2613746041894487 2307 96-97 -0.04874438646848489 2307 98-99 -0.09408097997038567 2307 100-101 -0.08192419882070467 2308 1 -0.5677002306863699 2308 2 -0.4017870712511957 2308 3 -0.5777680580139659 2308 4 -0.3848662360239601 2308 5 -0.3952673415263419 2308 6 -0.3840211119965531 2308 7 -0.42637944649877824 2308 8 -0.42597166352209825 2308 9 -0.5929803454123146 2308 10-11 -0.6654274649824714 2308 12-13 -0.5967314566509856 2308 14-15 -0.67504046405125 2308 16-17 -0.7993228061626354 2308 18-19 -0.7598734826297147 2308 20-21 -0.7119371176604616 2308 22-23 -0.8651004086684537 2308 24-25 -0.9478120592693386 2308 26-27 -0.8951699142125165 2308 28-29 -0.8728761309727133 2308 30-31 -0.8046888723526848 2308 32-33 -0.8220478987791253 2308 34-35 -0.7525182492233498 2308 36-37 -0.8956529054005244 2308 38-39 -0.7882262288593367 2308 40-41 -0.762162234940682 2308 42-43 -0.8177743441563976 2308 44-45 -0.797254088754265 2308 46-47 -0.8456447507906617 2308 48-49 -1.004685086232051 2308 50-51 -1.003536467928818 2308 52-53 -0.9276384109162663 2308 54-55 -0.82962067630136 2308 56-57 -0.77567625866822 2308 58-59 -0.7717908186872435 2308 60-61 -0.7924903971348058 2308 62-63 -0.7235708947381383 2308 64-65 -0.9857121748634441 2308 66-67 -0.7812013661387525 2308 68-69 -0.7714877769018855 2308 70-71 -0.6888911870722438 2308 72-73 -0.5556242411789825 2308 74-75 -0.6733468292663431 2308 76-77 -0.8757306254067849 2308 78-79 -0.9810321333860514 2308 80-81 -0.7880354337425395 2308 82-83 -0.791753858008061 2308 84-85 -0.8239961316701425 2308 86-87 -0.7626297938230202 2308 88-89 -0.7528946928082583 2308 90-91 -0.7388698578009176 2308 92-93 -0.7770267327345692 2308 94-95 -0.6712474454076229 2308 96-97 -0.5651685516553258 2308 98-99 -0.6871653651200944 2308 100-101 -0.7665983226645245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 107.0 13 996.0 14 4926.0 15 12635.0 16 22577.0 17 32188.0 18 41010.0 19 49781.0 20 59917.0 21 65404.0 22 71419.0 23 82213.0 24 98967.0 25 124317.0 26 161144.0 27 208005.0 28 269974.0 29 346825.0 30 445178.0 31 569497.0 32 735988.0 33 977782.0 34 1374699.0 35 2058201.0 36 3214765.0 37 4376896.0 38 3434249.0 39 440956.0 40 40.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.814547674685265 10.178919636592068 14.573114652393294 39.43341803632938 2 22.89120962707831 18.982815420400176 31.085108054887538 27.040866897633975 3 28.26526538690764 20.435373040374497 20.89643088481251 30.402930687905354 4 30.323526604925977 26.64628352585806 18.03720727112793 24.99298259808803 5 29.782796347075447 28.420197834503703 20.777975402399328 21.019030416021526 6 24.652977405702014 32.13383088888913 21.12525602727002 22.087935678138837 7 21.30476232116731 18.588942567892254 36.87786948798073 23.228425622959712 8 23.025170143285028 21.771120813752184 27.086457253721747 28.117251789241042 9 23.557579275938725 21.113358094932533 28.893563348269026 26.43549928085972 10-11 26.50741071584631 28.83725303816088 20.95659479724269 23.698741448750123 12-13 24.885412382905336 23.397740357553094 26.128569969670647 25.588277289870927 14-15 25.110767727309764 24.348356106670824 25.594973118400866 24.945903047618543 16-17 25.637220113978458 24.497155465779933 24.809110538274595 25.056513881967014 18-19 25.51317872348878 24.670209698723504 24.793294171812885 25.023317405974826 20-21 25.295828810018495 24.72472546454709 24.872093337463895 25.107352387970515 22-23 25.497108216362502 24.85165316687016 24.705029563042473 24.946209053724868 24-25 25.46195641321633 24.902553911403764 24.71229331815958 24.923196357220327 26-27 25.328900808950483 24.982520049494152 24.709062101138546 24.97951704041682 28-29 25.56031663022341 24.63933160797755 24.622833210956824 25.177518550842215 30-31 25.311668612917977 24.718402979566825 24.734577222609914 25.235351184905287 32-33 25.22321087624365 24.847345832068786 24.807702336710072 25.121740954977493 34-35 25.54062332199088 24.75513157977312 24.586029493490912 25.118215604745085 36-37 25.450648520398005 24.72058266891099 24.618254729688164 25.210514081002838 38-39 25.32569429638878 24.817494207535066 24.671679926215784 25.18513156986037 40-41 25.570500818902435 24.696612416195805 24.511947012634792 25.220939752266975 42-43 25.41462197933721 24.64228539431696 24.682867944914573 25.26022468143126 44-45 25.34635377685496 24.773398971854046 24.723404526792386 25.156842724498606 46-47 25.494890724023318 24.72203465531115 24.610679687765213 25.17239493290032 48-49 25.407308432766744 24.64198708145816 24.719468872925972 25.231235612849122 50-51 25.275982498732795 24.754584399362656 24.72822119532105 25.241211906583494 52-53 25.495919626321985 24.74889708906616 24.50629247136799 25.248890813243875 54-55 25.39817872523214 24.681926794817347 24.600715235359612 25.319179244590906 56-57 25.27611969424616 24.74021153709757 24.702020184338693 25.281648584317583 58-59 25.46933427230529 24.63535352859531 24.549083146060187 25.34622905303921 60-61 25.375762900059623 24.6717621067141 24.614333247442893 25.338141745783382 62-63 25.274456085742603 24.818768724024817 24.69813675117613 25.20863843905645 64-65 25.496495036268474 24.661539005726777 24.565958751610943 25.276007206393807 66-67 25.3944415193754 24.63825318305587 24.619324875854137 25.347980421714595 68-69 25.28573320178183 24.767571505976896 24.683150126440168 25.26354516580111 70-71 25.492266577375123 24.621884073372797 24.533227880517167 25.352621468734917 72-73 25.40857407682198 24.709974812575073 24.58553919309683 25.295911917506114 74-75 25.33398102896991 24.81823107620636 24.617275828873293 25.230512065950432 76-77 25.50341246080723 24.679506579157216 24.516944725079707 25.300136234955843 78-79 25.37679076459049 24.62999582846252 24.639160452019773 25.35405295492722 80-81 25.366754801611833 24.755364974260996 24.639549442832898 25.238330781294273 82-83 25.525317830130405 24.64919641499843 24.514603000384685 25.31088275448648 84-85 25.40072547324116 24.63623385013456 24.616130799698933 25.34690987692535 86-87 25.328587023027893 24.768977059448325 24.656146384192947 25.246289533330835 88-89 25.44776602835349 24.671490775134732 24.507427416518553 25.373315779993227 90-91 25.371583474238772 24.63657236647644 24.598972514479662 25.392871644805126 92-93 25.287372149741135 24.77783567689925 24.622350862348547 25.31244131101107 94-95 25.47307117738339 24.710693269281585 24.426989302908236 25.38924625042679 96-97 25.398613149206867 24.626764611441484 24.520201874821616 25.454420364530034 98-99 25.364560893425804 24.725337476759744 24.52939502437183 25.380706605442626 100-101 25.52383326336684 24.634996896890136 24.386291691912174 25.454878147830843 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 126.5 2 50.5 3 44.0 4 44.5 5 57.5 6 67.0 7 68.0 8 76.5 9 100.5 10 149.5 11 228.0 12 335.0 13 477.5 14 684.5 15 977.0 16 1323.5 17 1720.5 18 2248.0 19 2917.5 20 3882.5 21 5394.5 22 7292.0 23 9717.0 24 12991.0 25 17500.0 26 23410.5 27 30967.0 28 40507.5 29 52580.0 30 68788.0 31 89165.5 32 114435.5 33 147921.0 34 188303.0 35 233742.0 36 284642.0 37 341907.0 38 397767.5 39 447315.5 40 492918.0 41 534147.5 42 569976.0 43 600641.0 44 621950.0 45 638789.0 46 651926.0 47 655726.0 48 660727.0 49 663601.0 50 661336.0 51 663343.0 52 662270.0 53 652490.5 54 638517.5 55 625237.0 56 604334.5 57 575576.5 58 556581.0 59 540181.5 60 511224.0 61 481400.0 62 449560.5 63 414445.5 64 382981.5 65 347875.5 66 312600.5 67 279063.5 68 243133.0 69 204802.0 70 169584.0 71 138394.5 72 114050.0 73 95757.5 74 79333.5 75 64249.5 76 51851.0 77 42144.0 78 32692.5 79 23455.0 80 16180.5 81 10330.0 82 5983.5 83 3568.0 84 2103.0 85 1254.5 86 819.0 87 525.0 88 320.0 89 199.0 90 137.0 91 105.5 92 71.5 93 49.0 94 38.5 95 28.5 96 17.5 97 17.5 98 13.5 99 5.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08142355700356954 2 0.004543412697313645 3 0.0 4 1.089174276753271E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 3.111926505009346E-5 32-33 3.656513643385982E-4 34-35 0.0 36-37 1.555963252504673E-5 38-39 0.0034049662508977264 40-41 0.006208293377493645 42-43 0.0010736146442282242 44-45 0.002691816426833084 46-47 0.004535632881051122 48-49 1.0373088350031152E-5 50-51 0.0 52-53 0.0016545075918299687 54-55 8.013210750399065E-4 56-57 6.146054847393458E-4 58-59 0.0 60-61 0.0014781650898794392 62-63 6.094189405643302E-4 64-65 2.5932720875077884E-5 66-67 7.520489053772586E-5 68-69 0.0 70-71 0.0 72-73 4.149235340012461E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.5932720875077884E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.555963252504673E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9280661E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.05849543387214 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24689525113128 45.428970106524766 2 12.010971722566094 13.946778938219417 3 3.9956428166394775 6.9594303067574135 4 1.8715568381754926 4.3463909657377595 5 1.0200827797477865 2.9612235705079226 6 0.6249384899391626 2.176979307875029 7 0.41415573056763366 1.6831681014451052 8 0.301447525983461 1.4001271848690258 9 0.210080663590037 1.0977270523008291 >10 1.175563886091227 12.600815801958332 >50 0.09063443951197156 3.594604494077855 >100 0.037400807650669714 3.549881496029228 >500 5.841841373753965E-4 0.2259182312557366 >1k 4.4864268333519835E-5 0.027984442441665813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.111926505009346E-5 0.0 1.0373088350031152E-5 0.0 2 0.0 1.2447706020037383E-4 0.0 1.2447706020037383E-4 0.0 3 0.0 1.296636043753894E-4 0.0 1.34850148550405E-4 0.0 4 1.555963252504673E-5 1.4003669272542058E-4 0.0 1.6078286942548288E-4 0.0 5 2.0746176700062305E-5 1.4522323690043613E-4 0.0 1.6078286942548288E-4 0.0 6 4.6678897575140185E-5 1.4522323690043613E-4 5.186544175015576E-6 1.6078286942548288E-4 5.186544175015576E-6 7 5.7051985925171345E-5 1.8671559030056074E-4 5.186544175015576E-6 1.6596941360049844E-4 5.186544175015576E-6 8 6.74250742752025E-5 2.3858103205071652E-4 5.186544175015576E-6 1.7115595777551402E-4 5.186544175015576E-6 9 8.81712509752648E-5 2.645137529257944E-4 5.186544175015576E-6 1.8671559030056074E-4 5.186544175015576E-6 10-11 1.4522323690043613E-4 2.9303974588838007E-4 5.186544175015576E-6 1.970886786505919E-4 5.186544175015576E-6 12-13 2.0486849491311527E-4 3.137859225884424E-4 5.186544175015576E-6 2.1264831117563863E-4 5.186544175015576E-6 14-15 3.137859225884424E-4 3.474984597260436E-4 5.186544175015576E-6 2.2302139952566977E-4 5.186544175015576E-6 16-17 4.330764386138006E-4 3.786177247761371E-4 5.186544175015576E-6 2.4895412040074766E-4 5.186544175015576E-6 18-19 5.238409616765733E-4 4.04550445651215E-4 5.186544175015576E-6 2.748868412758256E-4 1.0373088350031152E-5 20-21 6.301651172643926E-4 4.330764386138007E-4 5.186544175015576E-6 3.0600610632591904E-4 1.0373088350031152E-5 22-23 9.958164816029906E-4 4.590091594888785E-4 5.186544175015576E-6 3.3971864346352024E-4 1.0373088350031152E-5 24-25 0.0015818959733797508 4.927216966264798E-4 1.0373088350031152E-5 3.941773573011838E-4 1.0373088350031152E-5 26-27 0.002606238447945327 5.082813291515265E-4 1.0373088350031152E-5 4.149235340012461E-4 1.0373088350031152E-5 28-29 0.004429308725463302 5.212476895890654E-4 1.0373088350031152E-5 4.40856254876324E-4 1.296636043753894E-5 30-31 0.00677621996465785 5.471804104641433E-4 1.555963252504673E-5 4.40856254876324E-4 1.8152904612554517E-5 32-33 0.00997631772064246 5.601467709016822E-4 1.555963252504673E-5 4.460427990513396E-4 2.0746176700062305E-5 34-35 0.014182605046580095 5.860794917767601E-4 1.8152904612554517E-5 4.486360711388474E-4 2.0746176700062305E-5 36-37 0.019055363299007227 6.094189405643302E-4 2.0746176700062305E-5 4.5382261531386295E-4 2.0746176700062305E-5 38-39 0.023513198017433116 6.223853010018691E-4 2.0746176700062305E-5 4.590091594888785E-4 2.0746176700062305E-5 40-41 0.028046237626396728 6.379449335269159E-4 2.0746176700062305E-5 4.719755199264175E-4 2.3339448787570093E-5 42-43 0.03192058612513336 6.638776544019937E-4 2.0746176700062305E-5 4.823486082764486E-4 2.852599296258567E-5 44-45 0.03548892851754408 7.079632798896263E-4 2.0746176700062305E-5 4.953149687139876E-4 3.111926505009346E-5 46-47 0.03954739933449377 7.624219937272898E-4 2.0746176700062305E-5 5.212476895890654E-4 3.111926505009346E-5 48-49 0.044503142293721154 8.013210750399066E-4 2.0746176700062305E-5 5.316207779390966E-4 3.111926505009346E-5 50-51 0.04977785771971199 8.194739796524611E-4 2.0746176700062305E-5 5.419938662891278E-4 3.111926505009346E-5 52-53 0.05668892783292025 8.583730609650779E-4 2.0746176700062305E-5 5.445871383766356E-4 3.111926505009346E-5 54-55 0.06768180821186577 9.050519585402182E-4 2.0746176700062305E-5 5.523669546391588E-4 3.111926505009346E-5 56-57 0.07479774681998713 9.283914073277882E-4 2.0746176700062305E-5 5.705198592517134E-4 3.371253713760125E-5 58-59 0.08102419310209333 9.828501211654518E-4 2.0746176700062305E-5 5.938593080392835E-4 4.149235340012461E-5 60-61 0.08698353235918624 0.001019155930390561 2.0746176700062305E-5 6.249785730893769E-4 4.149235340012461E-5 62-63 0.0936612079845188 0.0010736146442282242 2.0746176700062305E-5 6.586911102269782E-4 4.149235340012461E-5 64-65 0.09973983775763705 0.0011125137255408413 2.593272087507788E-5 6.924036473645794E-4 4.927216966264797E-5 66-67 0.10680909746818328 0.0011358531743284113 2.593272087507788E-5 7.183363682396573E-4 5.186544175015576E-5 68-69 0.11381093210445431 0.0011488195347659502 2.593272087507788E-5 7.494556332897508E-4 5.186544175015576E-5 70-71 0.12026299305817367 0.0011851253439910591 2.593272087507788E-5 7.987278029523987E-4 5.186544175015576E-5 72-73 0.13445856446519133 0.001288856227491371 2.852599296258567E-5 8.557797888775701E-4 5.186544175015576E-5 74-75 0.14243806267845277 0.0013614678459415888 3.111926505009346E-5 8.817125097526481E-4 5.186544175015576E-5 76-77 0.15101920001601604 0.00139518038307919 3.111926505009346E-5 8.972721422776947E-4 5.186544175015576E-5 78-79 0.16185129752553606 0.0014107400156042368 3.111926505009346E-5 9.102385027152337E-4 5.186544175015576E-5 80-81 0.171988398115604 0.0014418592806543303 3.111926505009346E-5 9.18018318977757E-4 5.186544175015576E-5 82-83 0.18330284423340049 0.0014626054573543927 3.111926505009346E-5 9.257981352402804E-4 5.186544175015576E-5 84-85 0.19560532701653743 0.0015040978107545172 3.111926505009346E-5 9.387644956778193E-4 5.445871383766355E-5 86-87 0.20987610331409282 0.0016078286942548287 3.111926505009346E-5 9.750703049029284E-4 5.7051985925171345E-5 88-89 0.2273262311909327 0.0016545075918299689 3.111926505009346E-5 0.0010061895699530218 5.7051985925171345E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCTA 1385 0.0 10.974424 4 CGTATCG 1990 0.0 8.354039 3 CGCGAAT 925 6.055161E-7 8.22294 1 TACCGTA 1815 0.0 8.112722 9 GTCGGAT 2230 0.0 8.100795 1 CCGGGAT 6345 0.0 8.0168 1 TACGCAC 1670 0.0 7.9638515 9 CGGCGAA 3060 0.0 7.9231453 1 GTCGGTT 2845 0.0 7.6864257 1 CGGGTTT 4285 0.0 7.6550345 1 CCCGTAT 2845 0.0 7.5193295 1 CGGGGTT 6940 0.0 7.32948 1 GTCCGTT 2240 0.0 7.215721 1 CCCGGAT 7325 0.0 7.1389437 1 GTCGATT 1680 2.4556357E-10 7.0742373 1 CCGGAAT 4240 0.0 7.0635595 1 CGGGGTA 3675 0.0 6.985304 1 GCGGTAT 2050 1.8189894E-12 6.9569087 1 GCCGTAT 2350 0.0 6.8779645 1 GCGAACG 1620 6.188202E-9 6.74364 3 >>END_MODULE