FastQCFastQC Report
Thu 26 May 2016
SRR1562283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17773859
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT7905.467553E-68.4163671
CCGCAAT26300.07.94548941
GCGGGTT33250.07.7130441
CGACCTA13054.816684E-97.64244754
CGGGGAT69900.07.5417041
CCCGTAT26650.07.4847241
CGGCGAT42200.07.31517361
CGATTCG9204.1645406E-57.22709753
GTTCGAT27050.07.1984711
GCGAACG15956.002665E-107.14618543
CGCTAGT8055.232257E-47.0796061
CCGGAAT39200.07.02693031
GCGGGAT46150.06.997791
GTCGGAT20601.8189894E-126.91636231
GTTAACG11750.06.871253516-17
CCGGGAT61050.06.8457451
CGGATAT32550.06.7116693
GTCGACG17052.5465852E-96.6851411
CCGGCAT55000.06.648931
CCGGTAT29550.06.58946371
Produced by FastQC (version 0.11.5)