##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1562283_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17773859 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06328901337633 31.0 31.0 33.0 28.0 34.0 2 30.760178023241885 31.0 31.0 33.0 27.0 34.0 3 31.00826370907972 31.0 31.0 34.0 27.0 34.0 4 34.77858567461349 37.0 35.0 37.0 32.0 37.0 5 34.73357220848888 37.0 35.0 37.0 32.0 37.0 6 34.64349711562357 37.0 35.0 37.0 31.0 37.0 7 34.62244361227351 37.0 35.0 37.0 31.0 37.0 8 34.71545374586352 37.0 35.0 37.0 32.0 37.0 9 36.25596517897436 39.0 35.0 39.0 32.0 39.0 10-11 36.39346790137134 39.0 35.0 39.0 32.0 39.0 12-13 36.31655477856553 39.0 35.0 39.0 32.0 39.0 14-15 37.3148222060274 39.0 37.0 41.0 32.0 41.0 16-17 37.346995157326276 39.0 37.0 41.0 32.0 41.0 18-19 37.233638373073624 39.0 36.5 41.0 31.0 41.0 20-21 37.2254595076961 39.0 36.5 41.0 31.0 41.0 22-23 37.10058167446923 39.0 36.0 41.0 31.0 41.0 24-25 36.85300060611486 39.0 36.0 41.0 30.0 41.0 26-27 36.68732791792711 39.0 36.0 40.5 30.0 41.0 28-29 36.77825825556509 39.0 36.0 40.0 30.0 41.0 30-31 36.61826643836885 39.0 36.0 40.0 30.0 41.0 32-33 36.579080125480914 39.0 35.0 40.0 30.0 41.0 34-35 36.389037068427285 39.0 35.0 40.0 30.0 41.0 36-37 36.169426543779835 38.0 35.0 40.0 29.0 41.0 38-39 36.00821391685396 38.0 34.5 40.0 28.0 41.0 40-41 36.029399214880684 38.0 34.5 40.0 28.5 41.0 42-43 36.24077534315986 38.0 35.0 40.0 29.0 41.0 44-45 36.22012313139201 38.0 35.0 40.0 29.0 41.0 46-47 36.23829403057603 38.0 35.0 40.0 29.0 41.0 48-49 36.05079583448929 38.0 34.5 40.0 28.0 41.0 50-51 35.644250750498244 37.5 33.5 39.5 28.0 40.5 52-53 35.53842409799695 38.0 34.0 40.0 28.0 41.0 54-55 35.62206299712403 38.0 34.0 40.0 28.0 41.0 56-57 35.4116031864549 37.5 34.0 40.0 28.0 41.0 58-59 35.03835824285542 37.0 33.5 40.0 27.0 41.0 60-61 34.912582658611164 37.0 34.0 40.0 27.0 41.0 62-63 34.68134275173445 36.0 33.5 39.5 26.5 41.0 64-65 34.37320637572291 36.0 33.0 39.0 26.0 41.0 66-67 34.06661637183011 35.0 33.0 39.0 26.0 41.0 68-69 33.813532559248955 35.0 33.0 38.5 26.0 40.0 70-71 33.558091211368335 35.0 33.0 37.5 26.0 40.0 72-73 33.31650810890308 35.0 33.0 37.0 26.0 39.5 74-75 32.99428272723442 35.0 33.0 36.5 26.0 39.0 76-77 32.61581331324841 35.0 32.0 36.0 25.0 39.0 78-79 32.34334243340177 35.0 32.0 36.0 25.0 37.5 80-81 32.04925672022041 35.0 32.0 35.0 25.0 37.0 82-83 31.79010289774438 35.0 32.0 35.0 24.0 37.0 84-85 31.527971190724536 35.0 32.0 35.0 24.0 36.0 86-87 31.34363809232424 35.0 32.0 35.0 24.0 36.0 88-89 31.09410615331201 35.0 31.0 35.0 23.0 36.0 90-91 30.869983580943227 34.0 31.0 35.0 21.5 35.0 92-93 30.66971362268599 34.0 31.0 35.0 20.0 35.0 94-95 30.473286808452798 34.0 31.0 35.0 19.5 35.0 96-97 30.21483373419357 34.0 31.0 35.0 17.5 35.0 98-99 29.90367271958217 34.0 31.0 35.0 6.5 35.0 100-101 29.438589475701363 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28610395229743446 1101 2 0.7797344494667513 1101 3 0.8176203815893537 1101 4 0.7906671616902017 1101 5 0.6065805010581968 1101 6 0.459081447819095 1101 7 0.5197173009630731 1101 8 0.6195601220882949 1101 9 0.6865693127959602 1101 10-11 0.850960775145154 1101 12-13 0.8700613051653363 1101 14-15 0.8714850497371103 1101 16-17 0.8507821505727478 1101 18-19 0.9302885792845501 1101 20-21 0.9081392574732376 1101 22-23 0.9139805055131092 1101 24-25 1.0369120477064726 1101 26-27 1.0827447436031292 1101 28-29 0.9742760153550662 1101 30-31 1.0162769770606488 1101 32-33 0.9709326304618031 1101 34-35 0.9859762501062121 1101 36-37 0.9949886718100842 1101 38-39 0.8563085231486056 1101 40-41 0.985382805867907 1101 42-43 1.1015112133619098 1101 44-45 1.1230540884219735 1101 46-47 0.998143150475812 1101 48-49 1.062088668401664 1101 50-51 1.013001968120527 1101 52-53 1.1123519904108008 1101 54-55 1.1207322159053277 1101 56-57 1.136018316269002 1101 58-59 1.2823926064819346 1101 60-61 1.1771590626929012 1101 62-63 1.2208504343655804 1101 64-65 1.2698036241214652 1101 66-67 1.1093772243224862 1101 68-69 1.0250993441849232 1101 70-71 0.9338568927910345 1101 72-73 0.8344164663786202 1101 74-75 0.7836483032525265 1101 76-77 0.8527733395435106 1101 78-79 0.8698054007293834 1101 80-81 0.8949385485240313 1101 82-83 0.8367990312705551 1101 84-85 0.9400518039765302 1101 86-87 0.932471631336071 1101 88-89 0.9584425742843621 1101 90-91 0.8578880051387863 1101 92-93 0.9699867564353646 1101 94-95 0.925258812572519 1101 96-97 0.9611612903632469 1101 98-99 1.0674174572103148 1101 100-101 1.0631402098021283 1102 1 0.47259595478780625 1102 2 0.8629096042974602 1102 3 0.8528074202592606 1102 4 0.7873316025478232 1102 5 0.6470416560906926 1102 6 0.5960376921818238 1102 7 0.7090285200876849 1102 8 0.6763112555149249 1102 9 0.7045812826171911 1102 10-11 0.859475008975096 1102 12-13 0.8589830910263601 1102 14-15 0.9301693486525551 1102 16-17 0.8178736576718251 1102 18-19 0.9279671397362463 1102 20-21 0.8370496671562577 1102 22-23 0.9919356448795611 1102 24-25 1.1292126314664088 1102 26-27 1.101500098945479 1102 28-29 1.0323009066907716 1102 30-31 1.0958577377668561 1102 32-33 0.9455800251284217 1102 34-35 0.9118423707486158 1102 36-37 0.8788599232520014 1102 38-39 0.8410040853074108 1102 40-41 0.9278638334835705 1102 42-43 0.9472204254616301 1102 44-45 1.0412944424307184 1102 46-47 0.7906610307349524 1102 48-49 0.8989484038791957 1102 50-51 0.9441697242191438 1102 52-53 0.9671041334707056 1102 54-55 0.9509674623147717 1102 56-57 0.9180224538569632 1102 58-59 1.162411097259934 1102 60-61 1.1467654063838424 1102 62-63 1.106853754660193 1102 64-65 1.2432292467241126 1102 66-67 1.2504665642788098 1102 68-69 1.1812136505044677 1102 70-71 1.0221843382108418 1102 72-73 0.9840776925127273 1102 74-75 1.0007595033045433 1102 76-77 1.1461352490935894 1102 78-79 1.1235574609342436 1102 80-81 1.0843134113022899 1102 82-83 1.1191376696471558 1102 84-85 1.1482737351940102 1102 86-87 1.1348703106745788 1102 88-89 1.194831811697739 1102 90-91 1.2148493470944466 1102 92-93 1.2619358499855728 1102 94-95 1.2926291875403457 1102 96-97 1.3536082989902845 1102 98-99 1.447929151201091 1102 100-101 1.4768502477490166 1103 1 0.28944561261325674 1103 2 0.6874370053382783 1103 3 0.5881371402840472 1103 4 0.5522012570928396 1103 5 0.520117464696618 1103 6 0.5001987984296221 1103 7 0.3932662734791279 1103 8 0.5407047416911084 1103 9 0.5005658467758991 1103 10-11 0.6049483233581441 1103 12-13 0.636093529491653 1103 14-15 0.6585241997846651 1103 16-17 0.6515631925811007 1103 18-19 0.66645891289118 1103 20-21 0.6401255974455466 1103 22-23 0.7029175659083933 1103 24-25 0.954996598462607 1103 26-27 0.8862944658565652 1103 28-29 0.7801082373016399 1103 30-31 0.7187932956132741 1103 32-33 0.7547284520625439 1103 34-35 0.8615174945577238 1103 36-37 0.8390573817268105 1103 38-39 0.7905581707669214 1103 40-41 0.7581583865516279 1103 42-43 0.8004361613908131 1103 44-45 0.8736002260744655 1103 46-47 0.6770010123085584 1103 48-49 0.6908191094898157 1103 50-51 0.614712067345458 1103 52-53 0.7067997704941789 1103 54-55 0.7576528076565339 1103 56-57 0.7595687666661775 1103 58-59 0.906093074982067 1103 60-61 0.8754845552155857 1103 62-63 0.8814371588439016 1103 64-65 0.9641940126278854 1103 66-67 0.9165910826983179 1103 68-69 0.7735443270348057 1103 70-71 0.7713656538361491 1103 72-73 0.7189322458735674 1103 74-75 0.7808476332707031 1103 76-77 0.8124610113623092 1103 78-79 0.8737424584752134 1103 80-81 0.8620716730500462 1103 82-83 0.8040245909686838 1103 84-85 0.8747985569562076 1103 86-87 0.8119852114824795 1103 88-89 0.8960088134527062 1103 90-91 0.9878064726205373 1103 92-93 0.9938265127826007 1103 94-95 0.866718368887927 1103 96-97 0.9640966841831151 1103 98-99 0.9780230129181469 1103 100-101 1.0041136589231066 1104 1 0.20087906231286468 1104 2 0.5357281773839162 1104 3 0.4248423634084837 1104 4 0.32301437609893924 1104 5 0.285662602845008 1104 6 0.15976131584275066 1104 7 0.32890030266109704 1104 8 0.31149451882015455 1104 9 0.28905443323453994 1104 10-11 0.3572375415395612 1104 12-13 0.23162236151686955 1104 14-15 0.3160952433786761 1104 16-17 0.20299466043318404 1104 18-19 0.22269717530982547 1104 20-21 0.26939460400960513 1104 22-23 0.24418300075758737 1104 24-25 0.4415135013107303 1104 26-27 0.42556646593006064 1104 28-29 0.30267805479947185 1104 30-31 0.19072039915558747 1104 32-33 0.42570063371297806 1104 34-35 0.5423426083049989 1104 36-37 0.49001312908175265 1104 38-39 0.44191105784162943 1104 40-41 0.6153340441398782 1104 42-43 0.5566258996661873 1104 44-45 0.5715566288000602 1104 46-47 0.47412834557872685 1104 48-49 0.5728366828458391 1104 50-51 0.4000598396133981 1104 52-53 0.5525058468462092 1104 54-55 0.5574495080957647 1104 56-57 0.5744726453769786 1104 58-59 0.7900868005127251 1104 60-61 0.7568875372754249 1104 62-63 0.7169776741144105 1104 64-65 0.6654478529210621 1104 66-67 0.6255462360185078 1104 68-69 0.7216849681772715 1104 70-71 0.6956302418305924 1104 72-73 0.517527134118339 1104 74-75 0.4089274049178755 1104 76-77 0.5931707111988729 1104 78-79 0.5782734451059142 1104 80-81 0.6897746650337027 1104 82-83 0.798804231804457 1104 84-85 0.806234442006236 1104 86-87 0.7574754446105771 1104 88-89 0.7694751940869153 1104 90-91 0.7713595199169632 1104 92-93 0.7182232338306669 1104 94-95 0.7812030041791367 1104 96-97 0.9265132555942976 1104 98-99 1.0232294197529974 1104 100-101 1.0093718016612776 1105 1 0.24593041561872653 1105 2 0.6424629243630733 1105 3 0.4883084194415588 1105 4 0.36885257360155776 1105 5 0.412407827434464 1105 6 0.4060122137691593 1105 7 0.48911673785385545 1105 8 0.5326938739621596 1105 9 0.5253052948459 1105 10-11 0.4799774233274263 1105 12-13 0.5198929922838005 1105 14-15 0.5531740869076529 1105 16-17 0.5081000019205604 1105 18-19 0.4935743190414499 1105 20-21 0.4793172895912079 1105 22-23 0.5517557501418082 1105 24-25 0.673752580784317 1105 26-27 0.6103882052463803 1105 28-29 0.5572475329709121 1105 30-31 0.6430888061769693 1105 32-33 0.6232399145890497 1105 34-35 0.5487739841842867 1105 36-37 0.5942553800968113 1105 38-39 0.6159974179967165 1105 40-41 0.6171387196255012 1105 42-43 0.653586037784244 1105 44-45 0.6596130991172444 1105 46-47 0.4551937476553647 1105 48-49 0.4890749449672498 1105 50-51 0.4739732497364031 1105 52-53 0.4613837619709571 1105 54-55 0.4784697153029356 1105 56-57 0.44627400392084837 1105 58-59 0.5775480467384995 1105 60-61 0.6513211353159463 1105 62-63 0.7252762517337388 1105 64-65 0.72250346475235 1105 66-67 0.739290535234332 1105 68-69 0.5692842354214918 1105 70-71 0.5872879720981032 1105 72-73 0.54083783354627 1105 74-75 0.5693217128501473 1105 76-77 0.5369270423026862 1105 78-79 0.6346791738528381 1105 80-81 0.6271370684495423 1105 82-83 0.6846970444818545 1105 84-85 0.6900620195128653 1105 86-87 0.6198678242034354 1105 88-89 0.7438045052939302 1105 90-91 0.8723169161516715 1105 92-93 0.9012744638254127 1105 94-95 0.8780044341692914 1105 96-97 0.9925487384842029 1105 98-99 1.106646981282033 1105 100-101 1.0667083462903442 1106 1 0.06720897790016878 1106 2 0.30286047744746725 1106 3 0.08035835574122174 1106 4 0.03658420210224733 1106 5 0.00988688459667486 1106 6 0.06304711631185 1106 7 0.10325183778061131 1106 8 0.0910802274930802 1106 9 -0.0848085455245382 1106 10-11 -0.07196366711958291 1106 12-13 -0.17827061804649702 1106 14-15 -0.17492654794151008 1106 16-17 -0.15822874047889712 1106 18-19 -0.18671651464683237 1106 20-21 -0.3149978725291902 1106 22-23 -0.3396924473810401 1106 24-25 -0.043458016699531754 1106 26-27 -0.14006390441652172 1106 28-29 -0.21955614738429574 1106 30-31 -0.09690383593876106 1106 32-33 -0.1319898629025431 1106 34-35 -0.08811803773502191 1106 36-37 -0.03791168605686579 1106 38-39 -0.042295920915783825 1106 40-41 -0.016572760781087936 1106 42-43 -0.041391219581377925 1106 44-45 -0.12089215969267997 1106 46-47 -0.13287176847278204 1106 48-49 -0.02954514083720028 1106 50-51 -0.13752076258965928 1106 52-53 -0.1850736677214968 1106 54-55 -0.1728653417539121 1106 56-57 -0.04387104668492725 1106 58-59 0.16361214269447544 1106 60-61 0.26557628520318843 1106 62-63 0.03218219593180294 1106 64-65 0.13022142524332025 1106 66-67 0.11327785370136212 1106 68-69 0.16082028979907648 1106 70-71 0.06561638719864504 1106 72-73 0.055537529881647174 1106 74-75 -0.029469476960514385 1106 76-77 0.11118613142233613 1106 78-79 0.11319917790333278 1106 80-81 0.07743581887591233 1106 82-83 0.049496644144081614 1106 84-85 0.1050374124737452 1106 86-87 0.13327477818651445 1106 88-89 0.0803722754182985 1106 90-91 -0.01690412640576966 1106 92-93 -0.009443945622241046 1106 94-95 0.030655232771845675 1106 96-97 0.031043622905986723 1106 98-99 0.054777077838512156 1106 100-101 0.12015480937102652 1107 1 -0.0880145114370805 1107 2 -0.04093980000478936 1107 3 -0.06941540421900427 1107 4 -0.1829068395569564 1107 5 -0.25822004371046603 1107 6 -0.13432329328578163 1107 7 -0.2001032039220334 1107 8 -0.2266678613837385 1107 9 -0.3493782893463404 1107 10-11 -0.44668935482391703 1107 12-13 -0.538477924447406 1107 14-15 -0.5299405432709179 1107 16-17 -0.6066217764301101 1107 18-19 -0.47165999877416454 1107 20-21 -0.517584872349687 1107 22-23 -0.5873099090769216 1107 24-25 -0.4914654123551898 1107 26-27 -0.6067731623943899 1107 28-29 -0.7048340709234324 1107 30-31 -0.7021233872443915 1107 32-33 -0.4502878646765964 1107 34-35 -0.4335251676811325 1107 36-37 -0.375585875506836 1107 38-39 -0.3567209539344418 1107 40-41 -0.45054497964495965 1107 42-43 -0.38772028761054145 1107 44-45 -0.5406822695569886 1107 46-47 -0.5458558808534022 1107 48-49 -0.6049310533042913 1107 50-51 -0.6248872489486175 1107 52-53 -0.5069640030155398 1107 54-55 -0.4326252295874369 1107 56-57 -0.553699118400985 1107 58-59 -0.5741190375555405 1107 60-61 -0.7256774350236057 1107 62-63 -0.7081707085638769 1107 64-65 -0.6370119116265229 1107 66-67 -0.4187216621131924 1107 68-69 -0.4606853531883388 1107 70-71 -0.5657157904718702 1107 72-73 -0.7219068831311191 1107 74-75 -0.5697338809940717 1107 76-77 -0.5110830419607097 1107 78-79 -0.7927635986139023 1107 80-81 -0.6708720878752956 1107 82-83 -0.6539723719262831 1107 84-85 -0.4524607470265174 1107 86-87 -0.39824843669784826 1107 88-89 -0.48191150855404885 1107 90-91 -0.5477187061880464 1107 92-93 -0.5129323455684833 1107 94-95 -0.5522076637858468 1107 96-97 -0.5991221476069484 1107 98-99 -0.6769731297091006 1107 100-101 -0.8651107151923831 1108 1 -1.4564762584459636 1108 2 -5.539391858015154 1108 3 -4.604634671287826 1108 4 -3.2017878548612835 1108 5 -2.3998126788955645 1108 6 -1.357124720118648 1108 7 -3.366678894611205 1108 8 -4.013299154213389 1108 9 -2.055986100175957 1108 10-11 -4.2320238671139165 1108 12-13 -3.3754275185917635 1108 14-15 -4.914610684492011 1108 16-17 -3.317402214073418 1108 18-19 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0.7370152421794494 2305 100-101 0.7072050133731196 2306 1 -0.09710831582032498 2306 2 0.22229176437631537 2306 3 -0.10595703602073314 2306 4 -0.11473574299684941 2306 5 -0.0992648884296159 2306 6 -0.33112391618595893 2306 7 -0.14894593667139588 2306 8 -6.133038649451805E-4 2306 9 -0.12926554545966695 2306 10-11 -0.11831884222165456 2306 12-13 -0.08772340948816293 2306 14-15 -0.31833037264237163 2306 16-17 -0.331336006223637 2306 18-19 -0.10937582552508474 2306 20-21 -0.14635923903789205 2306 22-23 -0.06798353782946265 2306 24-25 0.08619340403376441 2306 26-27 0.07270289196213042 2306 28-29 -0.17361812958043998 2306 30-31 -0.08576948826458164 2306 32-33 -0.10087827128307936 2306 34-35 0.007809298137317455 2306 36-37 -0.03038671060600251 2306 38-39 -0.24774204391443533 2306 40-41 -0.22198403443994863 2306 42-43 -0.0031956370959207447 2306 44-45 -0.1505637262278796 2306 46-47 -0.20070190377230546 2306 48-49 -0.09770261670031744 2306 50-51 -0.18491600884111392 2306 52-53 -0.5628678120448569 2306 54-55 -0.4339424728522019 2306 56-57 -0.1957414800005779 2306 58-59 -0.05044942275166875 2306 60-61 -0.0363095567448255 2306 62-63 -0.07499472326133372 2306 64-65 -0.04532484053633823 2306 66-67 0.008327390831425419 2306 68-69 -0.08904400186781913 2306 70-71 -0.04692318611343893 2306 72-73 -0.19293153067619784 2306 74-75 -0.15207716552997397 2306 76-77 -0.1542202310700702 2306 78-79 -0.15420736537505064 2306 80-81 -0.13629003581385746 2306 82-83 -0.20689121731014026 2306 84-85 -0.33209669821563637 2306 86-87 -0.36733666017967437 2306 88-89 -0.49234194771111817 2306 90-91 -0.4500711917860336 2306 92-93 -0.46973132527862305 2306 94-95 -0.9243926960847766 2306 96-97 -0.8141532694234748 2306 98-99 -0.5428253762341662 2306 100-101 -0.3108421311948 2307 1 -0.28128268175837334 2307 2 -0.0075961405052602515 2307 3 -0.09784954621899189 2307 4 -0.2534946163730041 2307 5 -0.2707451309996429 2307 6 -0.25302547287346044 2307 7 -0.2522991180730685 2307 8 -0.31460302275809227 2307 9 -0.4607822058080586 2307 10-11 -0.5133403678087447 2307 12-13 -0.5285751060062154 2307 14-15 -0.7558427009994446 2307 16-17 -1.1204952454474082 2307 18-19 -1.268864194276432 2307 20-21 -1.1051196435675905 2307 22-23 -0.7614800289566119 2307 24-25 -0.7612184632288219 2307 26-27 -1.5338149568360677 2307 28-29 -1.0242582128249822 2307 30-31 -0.7326746124343586 2307 32-33 -0.9550180044183634 2307 34-35 -0.7353930892135239 2307 36-37 -1.006555089456043 2307 38-39 -0.9318033597602167 2307 40-41 -0.7527471226661504 2307 42-43 -0.7337859463200473 2307 44-45 -1.179040652089661 2307 46-47 -1.043289947434161 2307 48-49 -0.9044090888606959 2307 50-51 -0.8975601184305404 2307 52-53 -1.0196173631193801 2307 54-55 -0.9215210931566986 2307 56-57 -0.7917891473935299 2307 58-59 -0.8820007309232523 2307 60-61 -1.1072380021596402 2307 62-63 -0.9924603066972324 2307 64-65 -0.9864901335274681 2307 66-67 -1.165642956239381 2307 68-69 -1.1888143654828127 2307 70-71 -1.2924040184668684 2307 72-73 -1.1773259844888457 2307 74-75 -1.1445690482329383 2307 76-77 -1.4542958820274237 2307 78-79 -1.1760847877027487 2307 80-81 -1.2148774898991235 2307 82-83 -1.107291332386314 2307 84-85 -0.967158224613776 2307 86-87 -0.9963626841720838 2307 88-89 -0.8861823233308534 2307 90-91 -0.9289520121339798 2307 92-93 -0.9862333897079729 2307 94-95 -1.3821731433460869 2307 96-97 -1.2728037013047668 2307 98-99 -1.5437983374317845 2307 100-101 -1.346566910438181 2308 1 -1.2459118244360283 2308 2 -1.2761609350044196 2308 3 -3.5760152973379 2308 4 -3.327443466146274 2308 5 -2.2838684421947875 2308 6 -1.3651623117116571 2308 7 -2.064322509479261 2308 8 -1.9466336825167687 2308 9 -2.5780317288859322 2308 10-11 -2.7214263118105535 2308 12-13 -3.051677385775676 2308 14-15 -2.5549592324633537 2308 16-17 -2.4690470278167496 2308 18-19 -3.2604925932208744 2308 20-21 -2.9978998483826302 2308 22-23 -2.785875140929022 2308 24-25 -4.137589894636591 2308 26-27 -4.3996204589362975 2308 28-29 -2.9501071762433355 2308 30-31 -2.8989521489157966 2308 32-33 -2.5754943263452716 2308 34-35 -3.9345737640481317 2308 36-37 -3.261788583017541 2308 38-39 -2.7636246058373857 2308 40-41 -3.999565532835902 2308 42-43 -3.290543743596949 2308 44-45 -2.8164495607084916 2308 46-47 -2.307469470590256 2308 48-49 -2.6836605690960695 2308 50-51 -2.3377300844088396 2308 52-53 -3.6939555773851964 2308 54-55 -4.247666280425271 2308 56-57 -3.5252112885395412 2308 58-59 -4.13814358003669 2308 60-61 -3.953471897207905 2308 62-63 -4.763748707429464 2308 64-65 -4.240748840635966 2308 66-67 -4.064157145506009 2308 68-69 -3.5919135728568534 2308 70-71 -2.657230852036072 2308 72-73 -2.660892821080754 2308 74-75 -2.276398107879505 2308 76-77 -2.80649319317407 2308 78-79 -2.403593388196782 2308 80-81 -2.694139889783898 2308 82-83 -2.5107662397279746 2308 84-85 -2.7474001998685296 2308 86-87 -2.8372301074352038 2308 88-89 -3.513309316528769 2308 90-91 -3.4912100917906557 2308 92-93 -3.392027384905777 2308 94-95 -3.195213299566806 2308 96-97 -3.141494508836292 2308 98-99 -3.4635495702803816 2308 100-101 -3.547792505717755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 817.0 11 6655.0 12 16982.0 13 29494.0 14 42136.0 15 53508.0 16 62242.0 17 69452.0 18 76096.0 19 86173.0 20 98962.0 21 113636.0 22 132079.0 23 154094.0 24 180672.0 25 211211.0 26 246748.0 27 289608.0 28 339068.0 29 402056.0 30 480954.0 31 580209.0 32 723436.0 33 939043.0 34 1293750.0 35 1914813.0 36 2868102.0 37 3587683.0 38 2480998.0 39 292950.0 40 229.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.84055432193988 9.553805957389445 15.813645196577738 39.791994524092935 2 23.256982248873598 18.89235302768819 30.968459408193766 26.882205315244445 3 29.390536233728486 20.19650324572262 20.613548417805163 29.799412102743727 4 30.721069238776245 25.856763318632144 17.977194687406058 25.444972755185557 5 29.85686451096523 28.298069653866385 20.52638090580104 21.318684929367336 6 24.728282136141623 32.11052253762112 20.63388147728639 22.527313848950868 7 21.35825990292823 18.833040140579488 36.34149455107077 23.467205405421524 8 23.10430166009531 21.95262717004788 26.661598924577945 28.281472245278866 9 23.521481744622818 21.2613366630173 28.514781173857628 26.702400418502254 10-11 26.462795727140627 28.806467970742876 20.757324000376055 23.97341230174044 12-13 24.825590773506192 23.4466161794127 25.876344579981197 25.851448467099914 14-15 25.095307102413717 24.35534399142021 25.337561752909142 25.211787153256925 16-17 25.581869981077265 24.462867067866352 24.684740663240323 25.270522287816057 18-19 25.44716372510888 24.68966362341459 24.650589385231424 25.212583266245108 20-21 25.235388547477665 24.734366189219195 24.70013614095871 25.330109122344428 22-23 25.40340602843064 24.856068883490178 24.569196873146847 25.17132821493233 24-25 25.363077103896853 24.959838921901543 24.554007025590032 25.123076948611573 26-27 25.22626793410261 25.0317483032384 24.547625183595535 25.194358579063454 28-29 25.40658799551037 24.68319976779381 24.486469834335633 25.423742402360183 30-31 25.174543646361876 24.774539198834184 24.602963151247888 25.44795400355605 32-33 25.11673395791926 24.886361881359957 24.660752418067087 25.336151742653694 34-35 25.40122115691517 24.795529104888704 24.488253806825 25.314995931371126 36-37 25.353842824841795 24.7528504231947 24.508328301597203 25.3849784503663 38-39 25.262476731155342 24.832508766009408 24.509968793332824 25.395045709502423 40-41 25.478392494320445 24.71980038986495 24.37916788638948 25.42263922942512 42-43 25.296993747953106 24.68093451174559 24.576581821651676 25.445489918649635 44-45 25.280888919696174 24.77869110624571 24.601588290571158 25.338831683486955 46-47 25.42429148970712 24.718324980785532 24.482684926060912 25.374698603446433 48-49 25.31368487314686 24.666277858800516 24.58451961602827 25.435517652024348 50-51 25.223184194659943 24.785365080786857 24.566603216170467 25.424847508382737 52-53 25.384335815446903 24.784568519396295 24.399283945433318 25.431811719723484 54-55 25.308518201924716 24.720498068709553 24.498113611831197 25.472870117534534 56-57 25.209170626313437 24.796287075094035 24.548996936372767 25.445545362219768 58-59 25.402016179136105 24.725612938920342 24.399994609974097 25.472376271969456 60-61 25.305804495777895 24.76834118038926 24.464384981365118 25.461469342467723 62-63 25.24430687696246 24.87486871891833 24.56041866807135 25.320405736047856 64-65 25.451841994677913 24.719842744318242 24.410903047098895 25.41741221390495 66-67 25.359802280416428 24.711501874747626 24.47916628572332 25.449529559112626 68-69 25.269593958183194 24.784828663263276 24.541201210159258 25.404376168394272 70-71 25.467328169982668 24.662809578943996 24.42559603966702 25.444266211406312 72-73 25.4115580640085 24.693843919882568 24.47976548030453 25.41483253580441 74-75 25.36779154149923 24.788421017630444 24.489785814099235 25.35400162677109 76-77 25.5582060147996 24.630633111245004 24.38970344031648 25.421457433638917 78-79 25.42815175878164 24.60009481351186 24.51219877215039 25.459554655556104 80-81 25.471246846281385 24.71290562167732 24.50118738986283 25.314660142178464 82-83 25.599140715175928 24.57594937011538 24.43824707538752 25.386662839321172 84-85 25.4968688559685 24.572373297745482 24.52067095179971 25.410086894486312 86-87 25.409694438663383 24.71858043448943 24.53866984712284 25.33305527972435 88-89 25.514425426689836 24.652994602916564 24.39911051392947 25.433469456464124 90-91 25.543436094507864 24.602961966323353 24.471872955030836 25.381728984137947 92-93 25.400916352903515 24.747377779238327 24.51003176449225 25.341674103365907 94-95 25.614069519232007 24.6275976477429 24.315284331409355 25.44304850161574 96-97 25.53181356126023 24.64389845124805 24.382159667072273 25.44212832041945 98-99 25.542018006672272 24.682246778639367 24.408082860579043 25.367652354109318 100-101 25.711455624024353 24.583755760132075 24.292385706492137 25.412402909351435 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 156.0 1 99.0 2 37.5 3 34.5 4 41.5 5 47.5 6 52.5 7 67.5 8 89.0 9 107.5 10 165.0 11 251.5 12 358.5 13 532.0 14 770.5 15 1055.0 16 1490.5 17 2150.0 18 3205.0 19 4184.5 20 4842.0 21 5918.0 22 7387.5 23 9263.0 24 11686.5 25 15023.5 26 19759.0 27 25622.5 28 33178.0 29 42913.5 30 56255.0 31 74243.0 32 96971.5 33 126995.5 34 163633.0 35 205656.5 36 253503.5 37 306824.0 38 359248.0 39 406533.5 40 448058.0 41 485103.0 42 521404.0 43 552857.0 44 572218.5 45 588559.0 46 603107.5 47 607835.5 48 611283.5 49 613374.5 50 613384.5 51 617129.0 52 616802.0 53 607482.0 54 594074.5 55 581675.0 56 563271.5 57 538111.0 58 519660.5 59 503691.0 60 477716.0 61 449749.5 62 419791.0 63 386897.5 64 356380.0 65 323457.5 66 290587.5 67 259336.5 68 225804.0 69 189863.0 70 157810.5 71 129565.5 72 106654.0 73 89455.5 74 74443.5 75 60366.5 76 49313.5 77 40385.0 78 30873.5 79 21727.0 80 14685.5 81 9042.5 82 4833.0 83 2602.0 84 1316.0 85 673.5 86 396.5 87 270.5 88 168.0 89 94.0 90 63.0 91 49.0 92 37.5 93 25.5 94 19.0 95 15.5 96 12.5 97 7.5 98 5.0 99 4.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 5.626240199159901E-6 3 2.8131200995799505E-5 4 1.0127232358487823E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.439360298739852E-6 22-23 4.4447297573363214E-4 24-25 6.779619439987681E-4 26-27 4.1915489483741264E-4 28-29 2.8131200995799503E-6 30-31 0.06290980478690643 32-33 1.9691840697059655E-5 34-35 3.263219315512743E-4 36-37 3.938368139411931E-5 38-39 5.232403385218708E-4 40-41 6.751488238991881E-5 42-43 0.0 44-45 0.0016034784567605716 46-47 7.90486747981966E-4 48-49 0.004093089744888828 50-51 0.0023798996042446383 52-53 0.003558596925968637 54-55 0.010878335425075669 56-57 0.00455162832112036 58-59 0.0016287965376567911 60-61 0.004157791507179167 62-63 0.005204272184222909 64-65 1.1252480398319801E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.1815104418235791E-4 80-81 0.0 82-83 4.782304169285916E-5 84-85 2.2504960796639602E-5 86-87 0.0017328819813412497 88-89 0.0 90-91 3.263219315512743E-4 92-93 0.015517170469283005 94-95 0.008338087975154973 96-97 1.772265662735369E-4 98-99 1.6878720597479703E-5 100-101 3.910236938416131E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7773859E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.73273197000616 #Duplication Level Percentage of deduplicated Percentage of total 1 79.822906100759 48.47863161284366 2 11.376637724117614 13.818685792373916 3 3.7036575554980646 6.747997248802565 4 1.673251756577522 4.064846018022585 5 0.8977914012591867 2.7262662268825215 6 0.5389227078070321 1.9638149019476654 7 0.3590272337911998 1.526329333184157 8 0.2601301917193835 1.2638733768799708 9 0.1947615315870057 1.0645559906347355 >10 1.064855573744428 12.027565927415287 >50 0.07783207303853634 3.2244025325967387 >100 0.029796502831571342 2.9220607104255016 >500 4.203627467303763E-4 0.16478462553641787 >1k 9.284522658791927E-6 0.006185702454381274 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.6262401991599014E-6 1.1252480398319803E-5 0.0 1.1252480398319803E-5 5.6262401991599014E-6 2 5.6262401991599014E-6 7.314112258907871E-5 0.0 1.1815104418235793E-4 5.6262401991599014E-6 3 1.1252480398319803E-5 7.876736278823862E-5 0.0 1.4065600497899754E-4 1.6878720597479703E-5 4 6.751488238991881E-5 8.439360298739851E-5 0.0 1.8003968637311685E-4 1.6878720597479703E-5 5 7.314112258907871E-5 8.439360298739851E-5 0.0 1.8566592657227673E-4 1.6878720597479703E-5 6 8.439360298739851E-5 9.564608338571832E-5 0.0 1.8566592657227673E-4 1.6878720597479703E-5 7 9.564608338571832E-5 1.4628224517815742E-4 0.0 1.8566592657227673E-4 1.6878720597479703E-5 8 1.1815104418235793E-4 2.0254464716975643E-4 0.0 1.8566592657227673E-4 1.6878720597479703E-5 9 1.2940352458067772E-4 2.3067584816555595E-4 0.0 2.0817088736891634E-4 1.6878720597479703E-5 10-11 1.7441344617395694E-4 2.475545687630357E-4 0.0 2.0817088736891634E-4 1.6878720597479703E-5 12-13 2.0254464716975646E-4 2.8693825015715496E-4 0.0 2.0817088736891634E-4 1.6878720597479703E-5 14-15 3.122563310533745E-4 3.460137722483339E-4 0.0 2.27862728065976E-4 1.6878720597479703E-5 16-17 3.8539745364245324E-4 3.741449732441334E-4 0.0 2.5599392906177547E-4 1.6878720597479703E-5 18-19 4.7823041692859165E-4 3.96649934040773E-4 0.0 2.8975137025673487E-4 1.6878720597479703E-5 20-21 5.823158606130497E-4 4.1915489483741264E-4 0.0 3.0944321095379454E-4 2.2504960796639606E-5 22-23 0.0011168086795332404 4.3603361543489234E-4 0.0 3.3476129185001414E-4 2.2504960796639606E-5 24-25 0.0018032099838307483 4.669779365302718E-4 5.6262401991599014E-6 4.0227617423993294E-4 2.2504960796639606E-5 26-27 0.0031985175532224038 4.8666977722733144E-4 5.6262401991599014E-6 4.163417747378327E-4 2.5318080896219557E-5 28-29 0.005803466765433437 5.119878581235509E-4 5.6262401991599014E-6 4.2759425513615244E-4 3.375744119495941E-5 30-31 0.009283296328613837 5.260534586214508E-4 8.439360298739852E-6 4.500992159327921E-4 3.375744119495941E-5 32-33 0.013826485289435458 5.373059390197705E-4 1.6878720597479703E-5 4.55725456131952E-4 3.375744119495941E-5 34-35 0.019885945983930668 5.457452993185103E-4 1.6878720597479703E-5 4.641648164306918E-4 3.375744119495941E-5 36-37 0.02674151966660701 5.766896204138898E-4 1.9691840697059655E-5 4.782304169285916E-4 3.375744119495941E-5 38-39 0.03355770966788923 6.076339415092693E-4 2.2504960796639606E-5 5.007353777252313E-4 3.375744119495941E-5 40-41 0.04017979438230043 6.132601817084292E-4 2.2504960796639606E-5 5.11987858123551E-4 3.6570561294539355E-5 42-43 0.04549940449060612 6.329520224054889E-4 2.2504960796639606E-5 5.176140983227109E-4 3.938368139411931E-5 44-45 0.050489879547260946 6.77961943998768E-4 2.2504960796639606E-5 5.232403385218708E-4 3.938368139411931E-5 46-47 0.055927640699748996 7.032800248949877E-4 2.2504960796639606E-5 5.288665787210307E-4 3.938368139411931E-5 48-49 0.06262849277694843 7.595424268865866E-4 2.2504960796639606E-5 5.513715395176703E-4 4.782304169285916E-5 50-51 0.0704349010532828 7.764211474840664E-4 3.094432109537946E-5 5.682502601151501E-4 5.063616179243911E-5 52-53 0.08032864444350549 8.326835494756653E-4 3.375744119495941E-5 5.738765003143099E-4 5.063616179243911E-5 54-55 0.09685291190843812 8.748803509693647E-4 3.375744119495941E-5 5.879421008122096E-4 5.063616179243911E-5 56-57 0.1073092793185768 9.283296328613837E-4 3.375744119495941E-5 6.188864219075891E-4 5.626240199159901E-5 58-59 0.11618748635285112 9.845920348529828E-4 3.375744119495941E-5 6.27325782206329E-4 6.188864219075892E-5 60-61 0.12435960024213086 9.930313951517227E-4 3.375744119495941E-5 6.582701033017084E-4 6.470176229033886E-5 62-63 0.13360913912954975 0.0010267888363466818 3.375744119495941E-5 6.864013042975079E-4 6.751488238991881E-5 64-65 0.14220603415386607 0.0010577331574420615 3.375744119495941E-5 7.173456253928874E-4 8.158048288781856E-5 66-67 0.15210259066418835 0.001088677478537441 3.375744119495941E-5 7.42663706289107E-4 8.439360298739851E-5 68-69 0.16192319293182195 0.0010971168388361806 3.375744119495941E-5 7.848605077828062E-4 8.439360298739851E-5 70-71 0.17057916347822946 0.0011590054810269395 3.375744119495941E-5 8.073654685794459E-4 8.439360298739851E-5 72-73 0.19160160998239043 0.0012349597237155982 3.375744119495941E-5 8.354966695752453E-4 8.720672308697847E-5 74-75 0.20143065161032278 0.0012799696453088775 3.375744119495941E-5 8.580016303718848E-4 9.001984318655842E-5 76-77 0.2125565416041615 0.0013193533267029968 3.6570561294539355E-5 8.664409906706247E-4 9.001984318655842E-5 78-79 0.22630707265090827 0.0013390451674000564 3.938368139411931E-5 8.692541107702047E-4 9.564608338571832E-5 80-81 0.23912083470449497 0.0013587370080971161 3.938368139411931E-5 9.058246720647441E-4 1.0127232358487821E-4 82-83 0.25298670367532455 0.0013812419688937557 3.938368139411931E-5 9.058246720647441E-4 1.0127232358487821E-4 84-85 0.2681747390929567 0.0014037469296903954 4.2196801493699264E-5 9.367689931601235E-4 1.0127232358487821E-4 86-87 0.2859452187620032 0.0014375043708853546 4.500992159327921E-5 9.67713314255503E-4 1.0689856378403812E-4 88-89 0.3070576851093507 0.0014628224517815743 4.782304169285916E-5 0.0010099101157492023 1.0689856378403812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATT 790 5.467553E-6 8.416367 1 CCGCAAT 2630 0.0 7.9454894 1 GCGGGTT 3325 0.0 7.713044 1 CGACCTA 1305 4.816684E-9 7.6424475 4 CGGGGAT 6990 0.0 7.541704 1 CCCGTAT 2665 0.0 7.484724 1 CGGCGAT 4220 0.0 7.3151736 1 CGATTCG 920 4.1645406E-5 7.2270975 3 GTTCGAT 2705 0.0 7.198471 1 GCGAACG 1595 6.002665E-10 7.1461854 3 CGCTAGT 805 5.232257E-4 7.079606 1 CCGGAAT 3920 0.0 7.0269303 1 GCGGGAT 4615 0.0 6.99779 1 GTCGGAT 2060 1.8189894E-12 6.9163623 1 GTTAACG 1175 0.0 6.8712535 16-17 CCGGGAT 6105 0.0 6.845745 1 CGGATAT 3255 0.0 6.711669 3 GTCGACG 1705 2.5465852E-9 6.685141 1 CCGGCAT 5500 0.0 6.64893 1 CCGGTAT 2955 0.0 6.5894637 1 >>END_MODULE