##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1562283_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17773859 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820520012001897 31.0 31.0 34.0 30.0 34.0 2 32.03972288741573 33.0 31.0 34.0 30.0 34.0 3 32.269644200508175 33.0 31.0 34.0 30.0 34.0 4 36.04118424704506 37.0 35.0 37.0 35.0 37.0 5 35.98255708003535 37.0 35.0 37.0 35.0 37.0 6 35.96732307823529 37.0 35.0 37.0 35.0 37.0 7 35.9472489907791 37.0 35.0 37.0 35.0 37.0 8 35.945719666168166 37.0 35.0 37.0 35.0 37.0 9 37.697304170129854 39.0 37.0 39.0 35.0 39.0 10-11 37.68217380367426 39.0 37.0 39.0 35.0 39.0 12-13 37.609432763025744 39.0 37.0 39.0 35.0 39.0 14-15 38.890689804617 40.0 38.0 41.0 35.5 41.0 16-17 38.82036559983963 40.0 38.0 41.0 35.0 41.0 18-19 38.8036831225003 40.0 38.0 41.0 35.0 41.0 20-21 38.7720858199674 40.0 38.0 41.0 35.0 41.0 22-23 38.64707385717418 40.0 38.0 41.0 34.5 41.0 24-25 38.557387875081034 40.0 38.0 41.0 34.0 41.0 26-27 38.3622254458078 40.0 38.0 41.0 34.0 41.0 28-29 38.19668308947426 40.0 38.0 41.0 34.0 41.0 30-31 38.073200029323964 40.0 37.5 41.0 33.5 41.0 32-33 37.89635269977105 40.0 37.0 41.0 33.0 41.0 34-35 37.73800121290486 39.5 37.0 41.0 33.0 41.0 36-37 37.7221653440595 39.5 37.0 40.5 33.0 41.0 38-39 37.853226612183654 40.0 37.0 41.0 33.0 41.0 40-41 37.814323467964954 40.0 37.0 41.0 33.0 41.0 42-43 37.65656822190387 39.5 37.0 41.0 33.0 41.0 44-45 37.481997212873125 39.0 36.0 41.0 32.0 41.0 46-47 37.34342730523518 39.0 36.0 40.5 32.0 41.0 48-49 37.1491593074976 39.0 35.0 40.0 31.0 41.0 50-51 36.928718659239955 39.0 35.0 40.0 31.0 41.0 52-53 36.682792324390554 38.5 35.0 40.0 31.0 41.0 54-55 36.396742879528865 38.0 35.0 40.0 30.5 41.0 56-57 36.10508500151824 38.0 34.5 40.0 30.0 41.0 58-59 35.78898226322151 37.0 34.0 40.0 29.5 41.0 60-61 35.47485256296902 37.0 34.0 40.0 29.0 41.0 62-63 35.213921158033266 36.0 34.0 39.0 29.0 41.0 64-65 34.95294859715045 36.0 34.0 39.0 29.0 41.0 66-67 34.65617745139083 35.5 33.5 39.0 29.0 40.5 68-69 34.30947421153729 35.0 33.0 38.5 28.0 40.0 70-71 34.0064465741514 35.0 33.0 37.5 28.0 40.0 72-73 33.655329183156 35.0 33.0 37.0 27.5 39.0 74-75 33.31791520344569 35.0 33.0 36.5 27.0 39.0 76-77 32.56666709238551 34.0 31.5 36.0 26.0 38.5 78-79 32.709899183964495 35.0 32.0 35.5 27.0 37.5 80-81 32.57586107215096 35.0 32.0 35.0 27.0 37.0 82-83 32.294635818816836 35.0 32.0 35.0 26.0 36.5 84-85 32.033110704884066 34.5 32.0 35.0 26.0 36.0 86-87 31.816955366867713 34.0 32.0 35.0 25.0 36.0 88-89 31.64554281655998 34.0 32.0 35.0 25.0 35.5 90-91 31.473753420683714 34.0 32.0 35.0 25.0 35.0 92-93 31.234980822116345 34.0 31.5 35.0 24.5 35.0 94-95 30.957528497328575 34.0 31.0 35.0 24.0 35.0 96-97 30.73544675919844 34.0 31.0 35.0 23.0 35.0 98-99 30.439086328973353 34.0 31.0 35.0 21.0 35.0 100-101 29.946450542901232 34.0 30.5 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3850016350079031 1101 2 0.4746551257962466 1101 3 0.4212994741212768 1101 4 0.26297958855604264 1101 5 0.2185638652860291 1101 6 0.23868249520384666 1101 7 0.2412375356220835 1101 8 0.26089902924218933 1101 9 0.26900089022190343 1101 10-11 0.24346137806298174 1101 12-13 0.3181554371201827 1101 14-15 0.37006701954803845 1101 16-17 0.41728658706062305 1101 18-19 0.4033759009984834 1101 20-21 0.3563977585696847 1101 22-23 0.3891646644437472 1101 24-25 0.3828410374926037 1101 26-27 0.3762609743029799 1101 28-29 0.3853612124230352 1101 30-31 0.2196736026166306 1101 32-33 0.2906814362300878 1101 34-35 0.17626370315871753 1101 36-37 0.2587769610573645 1101 38-39 0.3221611294674176 1101 40-41 0.3237995921735859 1101 42-43 0.3049132362220277 1101 44-45 0.31973112467565556 1101 46-47 0.31986186412263606 1101 48-49 0.30552861944234877 1101 50-51 0.26938663535579366 1101 52-53 0.3093414815959292 1101 54-55 0.24667292067099567 1101 56-57 0.23515445037691762 1101 58-59 0.16673065788751273 1101 60-61 0.18540594685429568 1101 62-63 0.20112366511732915 1101 64-65 0.17845705751773977 1101 66-67 0.1246740262888153 1101 68-69 0.06337365330130496 1101 70-71 -0.004252346495967174 1101 72-73 -0.07570412787725189 1101 74-75 -0.08701057742631946 1101 76-77 -0.20783107255910238 1101 78-79 -0.18986869291002506 1101 80-81 -0.2155797449429926 1101 82-83 -0.2404887782190741 1101 84-85 -0.32980772191887553 1101 86-87 -0.3587785486035884 1101 88-89 -0.30686814908922955 1101 90-91 -0.335094537621341 1101 92-93 -0.42595688458310477 1101 94-95 -0.553801546662239 1101 96-97 -0.7257788880315132 1101 98-99 -0.8250851540191135 1101 100-101 -0.8539505802208041 1102 1 0.26499574094128775 1102 2 0.3311525125535937 1102 3 0.3427495815514092 1102 4 0.19478009901335724 1102 5 0.18778998963077953 1102 6 0.14825474142888595 1102 7 0.17620615369163062 1102 8 0.15813499241743045 1102 9 0.17930474795669227 1102 10-11 0.17195225189286134 1102 12-13 0.1629150996743789 1102 14-15 0.2099369159356499 1102 16-17 0.23356453926707132 1102 18-19 0.22765494696423616 1102 20-21 0.23209121368441288 1102 22-23 0.2285736244861738 1102 24-25 0.2573568344525796 1102 26-27 0.26706432513311285 1102 28-29 0.25150290771322403 1102 30-31 0.23186221242568905 1102 32-33 0.264739746213408 1102 34-35 0.24251643309343507 1102 36-37 0.22513614732513787 1102 38-39 0.2814630957386157 1102 40-41 0.3062793815026623 1102 42-43 0.3407811678938657 1102 44-45 0.34663201391592224 1102 46-47 0.2573107573137179 1102 48-49 0.2801708507484335 1102 50-51 0.2831308119237548 1102 52-53 0.31470473441864044 1102 54-55 0.30894512208733005 1102 56-57 0.322127540722839 1102 58-59 0.30940634060394956 1102 60-61 0.22484073055853315 1102 62-63 0.28313424187624037 1102 64-65 0.33770039864573675 1102 66-67 0.3774006496299762 1102 68-69 0.37618556226937017 1102 70-71 0.3111092667772013 1102 72-73 0.20942211431050595 1102 74-75 0.22230823281065426 1102 76-77 0.1884526821235326 1102 78-79 0.2722035189685954 1102 80-81 0.1404529111234396 1102 82-83 0.1991081036147122 1102 84-85 0.05334609798845946 1102 86-87 0.1323456003593435 1102 88-89 0.2407863010874145 1102 90-91 0.22125091068591374 1102 92-93 0.18745406579620294 1102 94-95 0.07659544079509573 1102 96-97 -0.052905556578810575 1102 98-99 -0.04285821926037414 1102 100-101 0.010606303523875482 1103 1 0.23719475124125466 1103 2 0.3357540914017463 1103 3 0.29838740125378393 1103 4 0.18934201855932997 1103 5 0.1210645902125691 1103 6 0.09260448847332015 1103 7 0.11113154014638127 1103 8 0.14201743298592362 1103 9 0.15091363741132824 1103 10-11 0.1463726226615094 1103 12-13 0.15839023856478462 1103 14-15 0.18185449955149124 1103 16-17 0.23800481390438932 1103 18-19 0.18285642015408143 1103 20-21 0.19811702887517413 1103 22-23 0.24029399931031747 1103 24-25 0.2428168524665466 1103 26-27 0.17851470036280404 1103 28-29 0.20991389909099922 1103 30-31 0.17908622271219343 1103 32-33 0.09935853777721348 1103 34-35 0.13544345211165165 1103 36-37 0.14142399918814874 1103 38-39 0.13067099184085862 1103 40-41 0.07041970497494532 1103 42-43 0.07548178455357402 1103 44-45 0.11818097905093339 1103 46-47 0.013421278802361769 1103 48-49 2.866126844196515E-4 1103 50-51 -0.06142245881454045 1103 52-53 -0.06734277972119429 1103 54-55 -0.10221788570919443 1103 56-57 -0.06234900521739206 1103 58-59 -0.1385758555035963 1103 60-61 -0.2563801433438968 1103 62-63 -0.22924509165152784 1103 64-65 -0.39118164984223824 1103 66-67 -0.3590179139456495 1103 68-69 -0.3570213998248093 1103 70-71 -0.4187370515922879 1103 72-73 -0.5253394532677262 1103 74-75 -0.4864411733791698 1103 76-77 -0.602204029989359 1103 78-79 -0.5554785063865353 1103 80-81 -0.5109497972956021 1103 82-83 -0.3650564517526931 1103 84-85 -0.5084102495963734 1103 86-87 -0.5146346452660531 1103 88-89 -0.39552562996274077 1103 90-91 -0.3715230570402035 1103 92-93 -0.3076004287516305 1103 94-95 -0.3143204479665407 1103 96-97 -0.3401219548742134 1103 98-99 -0.3525889001638838 1103 100-101 -0.1976373490770662 1104 1 0.13482848448163764 1104 2 0.19136136431559692 1104 3 0.17695259403883767 1104 4 0.09800044140155251 1104 5 0.032658687642822315 1104 6 -0.06662790726397105 1104 7 -0.0768778035245603 1104 8 -0.009300546839142498 1104 9 -0.09097021904099023 1104 10-11 0.038672546353254234 1104 12-13 0.07806167069441727 1104 14-15 0.05247500157433649 1104 16-17 0.10343564623706669 1104 18-19 0.05894845567581797 1104 20-21 0.05456577788026351 1104 22-23 0.017783235462665914 1104 24-25 -0.10804114021299682 1104 26-27 -0.009069194209295972 1104 28-29 0.09719441635144221 1104 30-31 -8.902631994018861E-4 1104 32-33 0.04290573448483315 1104 34-35 -0.024682265783802393 1104 36-37 -0.1030467906383592 1104 38-39 -0.3486105382107638 1104 40-41 -0.21153998505423033 1104 42-43 -0.11872999291111341 1104 44-45 -0.3288327392005783 1104 46-47 -0.18538346075809642 1104 48-49 -0.2621043169014854 1104 50-51 -0.10758359245266291 1104 52-53 -0.29549551152398124 1104 54-55 -0.12867754100128082 1104 56-57 -0.18021996083810876 1104 58-59 -0.3384278122059783 1104 60-61 -0.23501935472189928 1104 62-63 0.007859339243118768 1104 64-65 -0.10775200599162105 1104 66-67 -0.2394112724882973 1104 68-69 -0.17279530048750047 1104 70-71 0.07396062736581399 1104 72-73 0.035634504022461044 1104 74-75 -0.0410938765190636 1104 76-77 -0.09688726030960026 1104 78-79 0.08323504005996085 1104 80-81 0.039433714453060986 1104 82-83 0.12902690642608405 1104 84-85 0.13339573823189 1104 86-87 0.16259689556361678 1104 88-89 0.30308686015058584 1104 90-91 0.20282588712552396 1104 92-93 0.32625804164776895 1104 94-95 0.28433430928631154 1104 96-97 0.1378492446804067 1104 98-99 0.2168770336125263 1104 100-101 0.28735801883928147 1105 1 0.08533878386638705 1105 2 0.14408556283508034 1105 3 0.15758786013019943 1105 4 0.07131342927818451 1105 5 0.06639485408710755 1105 6 0.0017831461170274565 1105 7 -0.0024200600742432243 1105 8 -0.037708345003977684 1105 9 -0.09656376191876603 1105 10-11 0.0034145440116120085 1105 12-13 -0.005810051112810299 1105 14-15 0.004030628646951584 1105 16-17 0.052793650255743785 1105 18-19 -0.037986560360877775 1105 20-21 0.02627634111706101 1105 22-23 0.029098496021468634 1105 24-25 0.040484543089363 1105 26-27 0.06885689108838022 1105 28-29 0.12139306322315235 1105 30-31 0.07186271551796608 1105 32-33 0.10797098481856438 1105 34-35 0.11265088450475247 1105 36-37 0.031570462071982774 1105 38-39 0.09134411352283678 1105 40-41 0.1213696397361872 1105 42-43 -0.011023910251800828 1105 44-45 0.06804831679960444 1105 46-47 0.12395350033694541 1105 48-49 0.1540500784518315 1105 50-51 0.12123745803143748 1105 52-53 0.21865050856393253 1105 54-55 0.21512337892075806 1105 56-57 0.2805748944797628 1105 58-59 0.29114887920580657 1105 60-61 0.23804914179757475 1105 62-63 0.32224872989019815 1105 64-65 0.3110602745567803 1105 66-67 0.37127461448862675 1105 68-69 0.37827449417681436 1105 70-71 0.4399206229614592 1105 72-73 0.36714023581031796 1105 74-75 0.4340401913105367 1105 76-77 0.40352506095701557 1105 78-79 0.5056906433297073 1105 80-81 0.48456717479749045 1105 82-83 0.6072361177640957 1105 84-85 0.557451200744616 1105 86-87 0.6115843236217486 1105 88-89 0.704692006251971 1105 90-91 0.7312157825351981 1105 92-93 0.7722249451738499 1105 94-95 0.7186377082177167 1105 96-97 0.6181013799397732 1105 98-99 0.6401740160205414 1105 100-101 0.8572722280402303 1106 1 -0.1135511330486203 1106 2 -0.05919776499068874 1106 3 -0.05903305577826501 1106 4 -0.07121349911490427 1106 5 -0.11728795643649192 1106 6 -0.16053526046221833 1106 7 -0.2214220109573617 1106 8 -0.19898644179276204 1106 9 -0.23050573741675606 1106 10-11 -0.25714419014891376 1106 12-13 -0.23439836654699775 1106 14-15 -0.28481832501034177 1106 16-17 -0.3096011139018131 1106 18-19 -0.31992920406214864 1106 20-21 -0.32198176688089575 1106 22-23 -0.2946818647754341 1106 24-25 -0.2709147062183703 1106 26-27 -0.3587035877984519 1106 28-29 -0.29068562120351515 1106 30-31 -0.26520770084119505 1106 32-33 -0.31714224185037665 1106 34-35 -0.25581901714328126 1106 36-37 -0.26713373403429586 1106 38-39 -0.2821015078021034 1106 40-41 -0.3565734765807278 1106 42-43 -0.4512913618218022 1106 44-45 -0.4241134376645803 1106 46-47 -0.41105892635368235 1106 48-49 -0.4112437585352495 1106 50-51 -0.33986579556462715 1106 52-53 -0.3437773980046188 1106 54-55 -0.3721858759428258 1106 56-57 -0.4940079339893586 1106 58-59 -0.4283512513092518 1106 60-61 -0.3319927875479749 1106 62-63 -0.4039613882361337 1106 64-65 -0.5605022701130551 1106 66-67 -0.26631632956984674 1106 68-69 -0.3067540308282233 1106 70-71 -0.39573333229560603 1106 72-73 -0.361905630977283 1106 74-75 -0.18592633632764688 1106 76-77 -0.2802553556964469 1106 78-79 -0.2264509036799467 1106 80-81 -0.23185581096856112 1106 82-83 -0.06419654215577708 1106 84-85 -0.1894958994226954 1106 86-87 -0.250923057794985 1106 88-89 -0.1892792383378996 1106 90-91 -0.17626252130013498 1106 92-93 -0.16231372663749255 1106 94-95 -0.21061388012543958 1106 96-97 -0.3164745258393893 1106 98-99 -0.32599697836718633 1106 100-101 -0.12005862899430042 1107 1 -0.2822806708679906 1107 2 -0.29268196026746907 1107 3 -0.32420269463366225 1107 4 -0.25986637795890744 1107 5 -0.28218507971118356 1107 6 -0.3087197994410218 1107 7 -0.3787128588664217 1107 8 -0.40604132113676883 1107 9 -0.4597996556673891 1107 10-11 -0.46038860613716537 1107 12-13 -0.548515782989746 1107 14-15 -0.748930405991679 1107 16-17 -0.7441676843917193 1107 18-19 -0.675286801622704 1107 20-21 -0.6221403434049222 1107 22-23 -0.607384220840764 1107 24-25 -0.6177928736538192 1107 26-27 -0.6264200170476428 1107 28-29 -0.6941716584097932 1107 30-31 -0.6280384803261754 1107 32-33 -0.6132062336538766 1107 34-35 -0.5871784084413676 1107 36-37 -0.6549550970043967 1107 38-39 -0.6314745523276315 1107 40-41 -0.6563944200192324 1107 42-43 -0.789744400033328 1107 44-45 -0.6724997908346921 1107 46-47 -0.8510981385869343 1107 48-49 -0.6116889804035495 1107 50-51 -0.6219245963939031 1107 52-53 -0.5785851135909255 1107 54-55 -0.59070556229846 1107 56-57 -0.422830301100241 1107 58-59 -0.4609261996912153 1107 60-61 -0.38403155211018714 1107 62-63 -0.6232463714509535 1107 64-65 -0.5399784882835306 1107 66-67 -0.3497653492484858 1107 68-69 -0.23585768682378472 1107 70-71 -0.2279897561512314 1107 72-73 -0.35001845380889307 1107 74-75 -0.24081722722841192 1107 76-77 -0.3550504625315085 1107 78-79 -0.2737968534551669 1107 80-81 -0.24714543548568457 1107 82-83 -0.24969523851023467 1107 84-85 -0.3814841452788045 1107 86-87 -0.22154008504535838 1107 88-89 -0.11898112956592755 1107 90-91 -0.13997780132758564 1107 92-93 -0.13417822479629748 1107 94-95 -0.13609536963587843 1107 96-97 -0.251488515139787 1107 98-99 -0.25571243912272834 1107 100-101 -0.098400896685785 1108 1 -0.4365509507049836 1108 2 -0.4625959598278726 1108 3 -0.4306653925964561 1108 4 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0.02628363767795605 2305 8 -0.026460856087261675 2305 9 0.02228495723524304 2305 10-11 0.08296077369558219 2305 12-13 0.07276211785703168 2305 14-15 0.07440761751270486 2305 16-17 0.08899417098124474 2305 18-19 0.053869703475953656 2305 20-21 -0.007989352432062446 2305 22-23 -0.02367787637720653 2305 24-25 -0.01224983500906518 2305 26-27 0.042162661357139086 2305 28-29 0.09492034395524485 2305 30-31 -0.025132265583984292 2305 32-33 0.004121013370919968 2305 34-35 0.0734723433172988 2305 36-37 0.05436944351976791 2305 38-39 0.00683146772300347 2305 40-41 0.02309652695241482 2305 42-43 0.10782735294419155 2305 44-45 0.16997287070982736 2305 46-47 0.24887548396132075 2305 48-49 0.20548099727405145 2305 50-51 0.2046488375992439 2305 52-53 0.23011927135251398 2305 54-55 0.29659296383047007 2305 56-57 0.30361144760039593 2305 58-59 0.15327343283430395 2305 60-61 0.3085131939271264 2305 62-63 0.3289358924430843 2305 64-65 0.29855585248461836 2305 66-67 0.18132786254199829 2305 68-69 0.32571333111344103 2305 70-71 0.4256033858431465 2305 72-73 0.3897713713448425 2305 74-75 0.40739891156845687 2305 76-77 0.47495739816554305 2305 78-79 0.46486472299029913 2305 80-81 0.47098035261082316 2305 82-83 0.4598122459210643 2305 84-85 0.532416151360998 2305 86-87 0.5270631595036974 2305 88-89 0.4518433281527301 2305 90-91 0.5248877141915038 2305 92-93 0.49838837717940976 2305 94-95 0.4582999449167282 2305 96-97 0.6023777074544512 2305 98-99 0.7024428148804382 2305 100-101 0.8151105591607255 2306 1 -1.1756792916097538 2306 2 -0.7010065246449564 2306 3 -0.34753481246965023 2306 4 -0.3326092547030086 2306 5 -0.2849244775469728 2306 6 -0.18554184157202513 2306 7 -0.16596939631021002 2306 8 -0.10220651181573714 2306 9 -0.1731841390676081 2306 10-11 -0.20899410747136216 2306 12-13 -0.17743261832852397 2306 14-15 -0.22820471069058357 2306 16-17 -0.1785249851711015 2306 18-19 -0.19761574948577731 2306 20-21 -0.18004427967566272 2306 22-23 -0.2024861943999312 2306 24-25 -0.1809517355248289 2306 26-27 -0.17161422096813084 2306 28-29 -0.1768269786953809 2306 30-31 -0.1567638431135876 2306 32-33 -0.12334341535859039 2306 34-35 -0.16717617778999738 2306 36-37 -0.1343805728253571 2306 38-39 -0.1946445661436229 2306 40-41 -0.14147932940650776 2306 42-43 -0.20372516043660482 2306 44-45 -0.13920296365234464 2306 46-47 -0.13686568662870258 2306 48-49 -0.10402313754898529 2306 50-51 -0.15671592288806835 2306 52-53 -0.24859361176469008 2306 54-55 -0.29189960922774816 2306 56-57 -0.1136796694939406 2306 58-59 -0.09990245860126379 2306 60-61 -0.10452489863829584 2306 62-63 -0.08510917284917952 2306 64-65 -0.1040006864881704 2306 66-67 -0.14930004973549416 2306 68-69 -0.09636319091162449 2306 70-71 -0.17862364906481787 2306 72-73 -0.10647701779824814 2306 74-75 -0.10976805586197003 2306 76-77 -0.09170182943433502 2306 78-79 -0.05823376484089238 2306 80-81 0.02458674932157834 2306 82-83 -0.06525702123840205 2306 84-85 0.062001320918220415 2306 86-87 -0.010758512081068261 2306 88-89 -0.018772061848526533 2306 90-91 -0.018107991623960373 2306 92-93 -0.17207356646986582 2306 94-95 0.014777456392902621 2306 96-97 0.14391827656206146 2306 98-99 0.11112553401287428 2306 100-101 0.16724856229457785 2307 1 -0.8800087166650101 2307 2 -1.2905498559424657 2307 3 -0.8168333613429652 2307 4 -0.4754889692057773 2307 5 -0.41361323204839806 2307 6 -0.40467737091190514 2307 7 -0.33127766187365637 2307 8 -0.32833046728062243 2307 9 -0.41638617191294003 2307 10-11 -0.36533483252299703 2307 12-13 -0.5653179807728961 2307 14-15 -0.6548752499146175 2307 16-17 -0.5944426010987982 2307 18-19 -0.5308020473797441 2307 20-21 -0.488883713532303 2307 22-23 -0.5284215026535009 2307 24-25 -0.4620415252940049 2307 26-27 -0.4260767301038655 2307 28-29 -0.4085075464890835 2307 30-31 -0.42823048210852477 2307 32-33 -0.45881817489379273 2307 34-35 -0.4502287375295211 2307 36-37 -0.37665564649030614 2307 38-39 -0.5766410738307286 2307 40-41 -0.6040195194401221 2307 42-43 -0.4310357369519551 2307 44-45 -0.4475469532853822 2307 46-47 -0.4814086529891384 2307 48-49 -0.45055837539472066 2307 50-51 -0.3750117140045788 2307 52-53 -0.34366364507983604 2307 54-55 -0.32213933293274266 2307 56-57 -0.2824359690114946 2307 58-59 -0.28978917756660394 2307 60-61 -0.3011237333494279 2307 62-63 -0.360875650458226 2307 64-65 -0.3926005011623914 2307 66-67 -0.290101497215133 2307 68-69 -0.26621070845803985 2307 70-71 -0.18273955070498715 2307 72-73 -0.06857911141739947 2307 74-75 -0.013272804104154545 2307 76-77 -0.04213200192369726 2307 78-79 -0.10629536048297439 2307 80-81 -0.1217761788783207 2307 82-83 -0.14800493381369506 2307 84-85 -0.047741134498245685 2307 86-87 -0.0654818197019793 2307 88-89 -0.07097028720005483 2307 90-91 -0.036708293337678555 2307 92-93 -0.21698118417124235 2307 94-95 -0.2486758493973511 2307 96-97 -0.06970419388993321 2307 98-99 -0.1288203473644245 2307 100-101 -0.09654379714790551 2308 1 -0.5717556985476904 2308 2 -0.4075628882273534 2308 3 -0.6048789189275432 2308 4 -0.3824409204935293 2308 5 -0.4192541419740792 2308 6 -0.403633799019282 2308 7 -0.4190986950420239 2308 8 -0.4147511712922025 2308 9 -0.6041166562027982 2308 10-11 -0.6935093130977634 2308 12-13 -0.6124985335635458 2308 14-15 -0.7210133099718874 2308 16-17 -0.8332477243449645 2308 18-19 -0.777479474366821 2308 20-21 -0.7447028441066124 2308 22-23 -0.8489139416766918 2308 24-25 -0.9601538310971947 2308 26-27 -0.8837709802271192 2308 28-29 -0.9084499945778717 2308 30-31 -0.8382196479233173 2308 32-33 -0.8794279343795068 2308 34-35 -0.7918442208045349 2308 36-37 -0.9274249702401107 2308 38-39 -0.8370113465446991 2308 40-41 -0.7712064574070183 2308 42-43 -0.8433575368342048 2308 44-45 -0.8255940108080324 2308 46-47 -0.891599317305527 2308 48-49 -1.0241665529858324 2308 50-51 -1.0308638074440424 2308 52-53 -0.9399224451410575 2308 54-55 -0.8714831775340315 2308 56-57 -0.8280148994526471 2308 58-59 -0.8188980578987355 2308 60-61 -0.8715235446545435 2308 62-63 -0.8013504249824237 2308 64-65 -1.023178011701141 2308 66-67 -0.8421709343260773 2308 68-69 -0.8601698094784567 2308 70-71 -0.7930773887892357 2308 72-73 -0.6298453528473544 2308 74-75 -0.761889305405056 2308 76-77 -0.9775024173099425 2308 78-79 -1.0949782031428938 2308 80-81 -0.8277036631361412 2308 82-83 -0.8268292003396631 2308 84-85 -0.9031105251495148 2308 86-87 -0.8666401279488873 2308 88-89 -0.8531851807221287 2308 90-91 -0.8239458100905388 2308 92-93 -0.8882605255614635 2308 94-95 -0.7797390664978288 2308 96-97 -0.6556502515735758 2308 98-99 -0.8327731964157792 2308 100-101 -0.9040364499428932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 95.0 13 976.0 14 4772.0 15 12090.0 16 21483.0 17 30505.0 18 38969.0 19 47288.0 20 56391.0 21 61347.0 22 67458.0 23 76776.0 24 92806.0 25 116648.0 26 150727.0 27 194177.0 28 250781.0 29 323773.0 30 412994.0 31 529286.0 32 683594.0 33 903911.0 34 1270348.0 35 1897398.0 36 2968169.0 37 4041657.0 38 3129374.0 39 390023.0 40 38.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.79168728226925 10.15658736142078 14.902711529596345 39.14901382671362 2 23.044742000880326 18.78319609416245 30.903927403986735 27.268134500970493 3 28.56353817142355 20.284897050212898 20.727963465896742 30.423601312466808 4 30.496201637428666 26.521912002936 17.93734759556501 25.044538764070325 5 29.914319675879053 28.349667902732882 20.616440132668995 21.11957228871907 6 24.75727415188789 32.06789251563208 21.000549177305842 22.17428415517418 7 21.4260842285291 18.655526636055793 36.62972683647372 23.288662298941386 8 23.16237571143104 21.722547703343434 26.935793740683998 28.179282844541525 9 23.66044987754207 21.024168133661913 28.773475698215 26.541906290581014 10-11 26.626305519808614 28.710000456288082 20.904275768137914 23.75941825576539 12-13 25.0130739756628 23.269966302759578 26.00454690227935 25.712412819298276 14-15 25.243603541583177 24.22621615260929 25.490570168245398 25.039610137562136 16-17 25.752916122492024 24.387981810815536 24.730597333983575 25.128504732708862 18-19 25.624570331068792 24.582042087764957 24.719544585112327 25.073842996053923 20-21 25.405408583470816 24.580674911396564 24.83003269014343 25.183883814989194 22-23 25.61481443056345 24.72702467145711 24.613821905529914 25.04433899244953 24-25 25.576924515942206 24.78362464786066 24.635235375727916 25.00421546046922 26-27 25.462078887876853 24.864200846872926 24.63145172919398 25.042268536056238 28-29 25.679268638285023 24.531107735241964 24.55793364851156 25.23168997796145 30-31 25.431868888319386 24.593814903969903 24.66251412538248 25.311802082328228 32-33 25.332127475039012 24.730558945926294 24.739831028117223 25.19748255091747 34-35 25.65471009981569 24.647829714413735 24.521174608170348 25.176285577600225 36-37 25.562323766801885 24.614375354365254 24.557263385283694 25.266037493549163 38-39 25.432205702389826 24.70150647743097 24.600376971009805 25.265910849169398 40-41 25.683488468392845 24.577162119548312 24.44366834949671 25.29568106256213 42-43 25.514715320903257 24.524383320452365 24.631758651740103 25.32914270690428 44-45 25.457830611493144 24.666749299835804 24.656849636120544 25.21857045255051 46-47 25.607822671102642 24.616454214828064 24.548421276004003 25.22730183806529 48-49 25.52189360131868 24.555924340003287 24.638691966558188 25.28349009211984 50-51 25.376793525817888 24.64288143615857 24.673083093547664 25.307241944475873 52-53 25.594727774247573 24.63727369939923 24.45369237082832 25.314306155524875 54-55 25.521923559020447 24.568512030936496 24.534408269927148 25.375156140115905 56-57 25.3748483684102 24.636225544593042 24.63005914873299 25.358866938263773 58-59 25.564076996447422 24.52905978380947 24.486384751898843 25.420478467844266 60-61 25.47804759553096 24.57057361266675 24.566137263239387 25.3852415285629 62-63 25.390609879381632 24.715214757228416 24.64095071921819 25.253224644171762 64-65 25.5924882695672 24.571401340418348 24.492062891931035 25.344047498083423 66-67 25.471168344009225 24.556231461176473 24.561033459888176 25.411566734926126 68-69 25.39617873642409 24.643964487396914 24.622784506167175 25.337072270011816 70-71 25.569579459362203 24.54977278710268 24.465668935485535 25.414978818049587 72-73 25.49327087455141 24.62045234418757 24.516400634527514 25.369876146733507 74-75 25.417583767261796 24.739236425809384 24.547494159822016 25.295685647106797 76-77 25.582978350396502 24.614553316755806 24.430246127191623 25.37222220565607 78-79 25.461527516337334 24.544444737634073 24.594920551580834 25.399107194447758 80-81 25.47105836723471 24.645359794966303 24.5844501185702 25.299131719228786 82-83 25.594433375441987 24.55893793238711 24.467674690116535 25.378954002054364 84-85 25.486432895014417 24.544549411046436 24.561881048367983 25.407136645571164 86-87 25.408789953830514 24.6748609854506 24.593719349298315 25.322629711420575 88-89 25.5173454453532 24.597404536628765 24.448210712147542 25.437039305870492 90-91 25.470290385447527 24.550546395130063 24.540005634116934 25.439157585305477 92-93 25.391424563455807 24.680928885505395 24.571504139871934 25.35614241116687 94-95 25.548478245495254 24.636304361365756 24.364295339577072 25.450922053561918 96-97 25.470844570107143 24.567585463573216 24.454194218599348 25.50737574772029 98-99 25.450604170990665 24.63011549714668 24.46651568463551 25.452764647227145 100-101 25.595553064256116 24.580283840474245 24.299779164421093 25.52438393084855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 168.5 2 75.5 3 74.0 4 68.5 5 69.5 6 76.0 7 91.5 8 111.5 9 141.5 10 202.5 11 292.5 12 426.0 13 622.5 14 889.5 15 1227.5 16 1678.5 17 2161.5 18 2674.5 19 3359.5 20 4288.0 21 5554.0 22 7145.0 23 9281.5 24 12102.0 25 15489.0 26 19853.5 27 25932.5 28 33642.0 29 43471.5 30 56914.5 31 74296.0 32 96117.0 33 125615.5 34 162562.0 35 204307.5 36 251414.0 37 304037.0 38 355409.0 39 402256.5 40 444459.0 41 483465.0 42 518098.5 43 547383.5 44 568253.0 45 585486.5 46 600761.0 47 606202.5 48 610324.5 49 613712.5 50 615146.5 51 620470.5 52 621229.0 53 612989.0 54 600857.0 55 588394.0 56 568959.0 57 542961.5 58 523458.5 59 506597.0 60 480067.0 61 450883.0 62 419997.0 63 386737.0 64 356239.0 65 323210.5 66 289533.5 67 258042.5 68 225028.5 69 188908.0 70 156520.5 71 128379.5 72 105784.0 73 88541.5 74 73421.5 75 59800.0 76 48628.0 77 39497.5 78 30540.0 79 21851.5 80 15113.5 81 9707.0 82 5520.0 83 3282.5 84 1952.0 85 1177.0 86 745.5 87 448.5 88 275.0 89 187.0 90 119.5 91 81.0 92 65.0 93 47.0 94 29.5 95 23.0 96 16.0 97 11.0 98 7.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08018517531842691 2 0.004906081453667434 3 0.0 4 9.001984318655841E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.2504960796639602E-5 32-33 4.135286546382527E-4 34-35 0.0 36-37 8.439360298739852E-6 38-39 0.00342638028128838 40-41 0.00629857590295951 42-43 0.001257464684512238 44-45 0.0029706548251564277 46-47 0.004785117289385496 48-49 8.439360298739852E-6 50-51 0.0 52-53 0.0014909536527773738 54-55 8.973853117660043E-4 56-57 5.935683410113696E-4 58-59 0.0 60-61 0.0013306058071013167 62-63 6.470176229033885E-4 64-65 2.8131200995799505E-5 66-67 5.626240199159901E-5 68-69 0.0 70-71 0.0 72-73 2.2504960796639602E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.5318080896219557E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.1252480398319801E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7773859E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.054738276115614 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84139281397294 46.55957817953594 2 11.764989696276839 13.89556774669651 3 3.9250920989111178 6.95385859832536 4 1.8125790253632132 4.28165519790405 5 0.9699588680610299 2.864033354597074 6 0.5718926299891688 2.0263781751629875 7 0.38875716402142557 1.6070566801975166 8 0.26638397106648554 1.2584988553826928 9 0.20106595183139922 1.0686507445477214 >10 1.1354024015198336 12.468023226236381 >50 0.08842493047600058 3.5669349378460518 >100 0.0335315872622255 3.240416308663083 >500 5.192986251182749E-4 0.20368951829504078 >1k 9.56262344046863E-6 0.00565847660958943 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.6262401991599014E-6 2.2504960796639606E-5 0.0 1.1252480398319803E-5 0.0 2 5.6262401991599014E-6 6.751488238991881E-5 0.0 5.626240199159901E-5 0.0 3 1.6878720597479703E-5 7.314112258907871E-5 5.6262401991599014E-6 7.314112258907871E-5 0.0 4 7.876736278823862E-5 7.876736278823862E-5 5.6262401991599014E-6 7.314112258907871E-5 0.0 5 8.439360298739851E-5 8.439360298739851E-5 5.6262401991599014E-6 7.314112258907871E-5 0.0 6 1.0127232358487821E-4 8.439360298739851E-5 5.6262401991599014E-6 7.314112258907871E-5 0.0 7 1.1815104418235793E-4 1.2940352458067772E-4 5.6262401991599014E-6 7.314112258907871E-5 0.0 8 1.4065600497899754E-4 1.5190848537731733E-4 5.6262401991599014E-6 9.001984318655842E-5 0.0 9 1.4628224517815742E-4 1.8566592657227673E-4 5.6262401991599014E-6 9.564608338571832E-5 0.0 10-11 1.9129216677143664E-4 1.8566592657227673E-4 5.6262401991599014E-6 1.0127232358487823E-4 0.0 12-13 2.3630208836471586E-4 1.9691840697059652E-4 5.6262401991599014E-6 1.1252480398319802E-4 0.0 14-15 3.572662526466537E-4 2.27862728065976E-4 5.6262401991599014E-6 1.2659040448109778E-4 0.0 16-17 4.332204953353124E-4 2.8693825015715496E-4 5.6262401991599014E-6 1.5753472557647724E-4 0.0 18-19 5.457452993185103E-4 3.178825712525344E-4 5.6262401991599014E-6 1.8847904667185667E-4 2.8131200995799507E-6 20-21 6.638963435008683E-4 3.347612918500141E-4 5.6262401991599014E-6 1.9691840697059655E-4 5.6262401991599014E-6 22-23 0.0012855958855080374 3.488268923479139E-4 5.6262401991599014E-6 2.1379712756807622E-4 1.1252480398319803E-5 24-25 0.0020226333515979845 3.825843335428733E-4 5.6262401991599014E-6 2.5880704916135544E-4 1.1252480398319803E-5 26-27 0.0034573246023837595 3.96649934040773E-4 5.6262401991599014E-6 2.7287264965925517E-4 2.2504960796639606E-5 28-29 0.006183237978876731 4.079024144390928E-4 5.6262401991599014E-6 2.7568576975883514E-4 2.5318080896219557E-5 30-31 0.009845920348529828 4.332204953353124E-4 5.6262401991599014E-6 2.7568576975883514E-4 3.375744119495941E-5 32-33 0.014695739400205663 4.55725456131952E-4 1.1252480398319803E-5 2.7568576975883514E-4 3.375744119495941E-5 34-35 0.02110684010714837 4.613516963311119E-4 1.1252480398319803E-5 2.813120099579951E-4 3.375744119495941E-5 36-37 0.02831686692237178 4.810435370281715E-4 1.1252480398319803E-5 2.813120099579951E-4 3.375744119495941E-5 38-39 0.03529340476933006 4.951091375260713E-4 1.1252480398319803E-5 2.8412513005757504E-4 3.375744119495941E-5 40-41 0.04217992277310178 5.091747380239711E-4 1.6878720597479703E-5 2.953776104558948E-4 3.6570561294539355E-5 42-43 0.04770770376877638 5.232403385218708E-4 2.2504960796639606E-5 3.0381697075463466E-4 4.2196801493699264E-5 44-45 0.052872592271605165 5.429321792189305E-4 2.2504960796639606E-5 3.1788257125253444E-4 4.500992159327921E-5 46-47 0.05843975694867389 5.654371400155701E-4 2.2504960796639606E-5 3.291350516508542E-4 4.500992159327921E-5 48-49 0.06542754727603048 5.963814611109495E-4 2.2504960796639606E-5 3.4038753204917397E-4 5.063616179243911E-5 50-51 0.07340274275833965 6.413913827042287E-4 2.2504960796639606E-5 3.628924928458136E-4 5.063616179243911E-5 52-53 0.08355810631782326 6.948406645962478E-4 2.2504960796639606E-5 3.713318531445535E-4 5.626240199159901E-5 54-55 0.10054935171928617 7.454768263886869E-4 2.8131200995799505E-5 3.769580933437134E-4 5.9075522091178964E-5 56-57 0.11130109673988074 7.764211474840663E-4 3.375744119495941E-5 3.825843335428733E-4 6.188864219075892E-5 58-59 0.12034246473993071 8.326835494756653E-4 3.375744119495941E-5 3.9383681394119304E-4 6.188864219075892E-5 60-61 0.1286214771929945 8.720672308697847E-4 3.375744119495941E-5 4.3603361543489234E-4 6.188864219075892E-5 62-63 0.13805668200698565 9.255165127618038E-4 3.375744119495941E-5 4.55725456131952E-4 6.188864219075892E-5 64-65 0.14685893479857132 9.705264343550829E-4 3.375744119495941E-5 5.063616179243911E-4 6.188864219075892E-5 66-67 0.15696084907616292 9.902182750521427E-4 3.938368139411931E-5 5.260534586214508E-4 6.188864219075892E-5 68-69 0.16684615310608686 9.958445152513026E-4 3.938368139411931E-5 5.457452993185103E-4 6.188864219075892E-5 70-71 0.17565403213787167 0.0010296019564462618 3.938368139411931E-5 5.823158606130497E-4 6.188864219075892E-5 72-73 0.1968086952867129 0.0011055561991349205 3.938368139411931E-5 6.30138902305909E-4 6.188864219075892E-5 74-75 0.2067024386769356 0.0011533792408277795 3.938368139411931E-5 6.498307430029685E-4 6.188864219075892E-5 76-77 0.21788740419286548 0.0011815104418235792 3.938368139411931E-5 6.610832234012884E-4 6.470176229033886E-5 78-79 0.23175327316369507 0.001206828522719799 3.938368139411931E-5 6.695225837000282E-4 7.595424268865866E-5 80-81 0.24452202529568845 0.0012208941232176986 3.938368139411931E-5 6.892144243970878E-4 7.876736278823862E-5 82-83 0.2585341765116962 0.0012405859639147581 3.938368139411931E-5 7.032800248949877E-4 8.158048288781856E-5 84-85 0.27379253993181785 0.001305287726205097 3.938368139411931E-5 7.173456253928873E-4 8.439360298739851E-5 86-87 0.29145612103708035 0.0013671763683958558 3.938368139411931E-5 7.96112988181126E-4 8.439360298739851E-5 88-89 0.3123716689774573 0.0013896813291924956 3.938368139411931E-5 8.49562270073145E-4 8.439360298739851E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGTAT 2475 0.0 9.027819 1 CGACCTA 1315 9.094947E-12 8.668907 4 CGCGGAT 2725 0.0 7.8506613 1 CCGGGAT 5800 0.0 7.786742 1 GTATCGA 2345 0.0 7.6969666 4 GCCGTAT 2165 0.0 7.6854687 1 GCGGGAT 4795 0.0 7.535033 1 GTCGGAT 2085 0.0 7.5243344 1 GTCGGGA 5050 0.0 7.342829 1 GCCGGAT 4485 0.0 7.2078657 1 GTCGGTT 2780 0.0 7.182319 1 TATGGCG 3515 0.0 7.1619186 7 CGCCTAT 1755 8.913048E-11 7.0429797 1 GTTAACG 1215 0.0 7.0367985 16-17 GTCCGTT 2040 1.8189894E-12 6.9911933 1 CCGCAAT 3030 0.0 6.9035144 1 GCGGTGT 3790 0.0 6.8989606 1 CGCAATC 1800 1.6370905E-10 6.861322 2 CGCTAAT 1125 1.1370521E-5 6.7612605 1 CGGATCT 6430 0.0 6.7221665 3 >>END_MODULE