FastQCFastQC Report
Tue 31 May 2016
SRR1104743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1104743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044279
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT821841.021645320854734No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT81750.10162501822723952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG906150.093.828451
TCGACGG920000.092.42112
CGACGGC934100.090.7523
ACGGCGC939250.090.320145
GACGGCG942300.090.068134
CGGCGCG949600.089.3073656
TATCTCG220150.088.4168242
ATATCTC224950.086.656861
ATCTCGA228100.085.2732853
TCTCGAG226650.084.226094
TCGAGGG231400.082.293436
CTCGAGG234500.081.345815
AGGGCGC236900.080.783879
CGAGGGC241150.078.94657
GAGGGCG253300.075.5909968
CGCGCCG1166550.072.7675869
GGCGCGC1179550.072.0421147
GCGCGCC1179550.071.9736568
CCATATT992800.043.1752818-19
TCCATAT997200.042.94428618-19
Produced by FastQC (version 0.11.5)