FastQCFastQC Report
Tue 31 May 2016
SRR1104743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1104743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044279
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490970.6103343755232756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG55700.090.402551
TCGACGG64900.077.580052
CGACGGC82800.060.5790443
ACGGCGC85650.058.785115
GACGGCG89150.056.477234
CGGCGCG99050.050.9762236
TATCTCG38300.049.2357562
ATATCTC40000.047.6677061
CGCGCCG164950.033.8068439
ATCTCGA57400.033.1834343
GGCGCGC180750.030.9042267
GCGCGCC181850.030.7956498
TCTCGAG67000.028.3578994
TCGAGGG76000.024.8122276
CTCGAGG78500.024.1430475
AGGGCGC81250.023.3843619
CCATATT128800.023.17812518-19
CGAGGGC86450.021.9777817
ATCCATA142450.020.97379716-17
TCCATAT134750.019.42279218-19
Produced by FastQC (version 0.11.5)