FastQCFastQC Report
Tue 31 May 2016
SRR1051302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14610788
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT5202.4011206E-711.8773771
CGGGGAT60050.07.67430831
GCCGGGA77250.07.3186161
CCCGGAT58650.07.20945551
CCCCGAT32200.07.08216141
CGGCGAT22250.07.0463531
CGGGGTA22400.06.9991681
TCTAACG10302.4383384E-56.9196417
CCCCCGT32400.06.89181041
CCGGGAT50750.06.8338791
CGGGGTT42150.06.76291851
GCCGGGT58850.06.7005761
GTCGGTT26250.06.69657751
CGGGATA16559.587893E-96.6025292
CCGGCAT44700.06.5896851
GCCCCGT45450.06.58547541
TCGTGCG14854.0495615E-76.3993097
CTCCCGT47900.06.14945551
GCGGGTT27200.06.1133551
CGGGGGA60200.06.0767971
Produced by FastQC (version 0.11.5)