##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14610788 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861415551303597 33.0 31.0 34.0 30.0 34.0 2 31.91223943568273 33.0 31.0 34.0 30.0 34.0 3 31.930201916556452 33.0 31.0 34.0 30.0 34.0 4 35.02091776295707 37.0 35.0 37.0 32.0 37.0 5 35.207041742033354 37.0 35.0 37.0 32.0 37.0 6 35.272519319286545 37.0 35.0 37.0 32.0 37.0 7 35.43535742220064 37.0 35.0 37.0 33.0 37.0 8 35.473225742512994 37.0 35.0 37.0 33.0 37.0 9 37.33377802757798 39.0 37.0 39.0 34.0 39.0 10-11 37.37996708322645 39.0 37.0 39.0 34.0 39.0 12-13 37.30407237446741 39.0 37.0 39.0 34.0 39.0 14-15 38.70210333624716 40.0 38.0 41.0 34.0 41.0 16-17 38.582262469347995 40.0 38.0 41.0 34.0 41.0 18-19 38.57959834199224 40.0 38.0 41.0 34.0 41.0 20-21 38.40229839075072 40.0 38.0 41.0 33.5 41.0 22-23 37.76569248010443 40.0 38.0 41.0 32.0 41.0 24-25 38.0403279754658 40.0 38.0 41.0 32.5 41.0 26-27 38.13938313936251 40.0 38.0 41.0 33.0 41.0 28-29 38.154617567512446 40.0 38.0 41.0 33.0 41.0 30-31 37.997620012007566 40.0 38.0 41.0 33.0 41.0 32-33 38.005222921583695 40.0 38.0 41.0 33.0 41.0 34-35 37.95036390234394 40.0 38.0 41.0 33.0 41.0 36-37 37.748163925176385 40.0 37.0 41.0 32.5 41.0 38-39 37.55807082410613 39.5 37.0 41.0 32.0 41.0 40-41 37.38052454802575 39.0 36.5 41.0 31.5 41.0 42-43 37.194776181818526 39.0 36.0 41.0 31.0 41.0 44-45 36.95058853088554 39.0 36.0 40.5 30.0 41.0 46-47 36.94274935752952 39.0 35.0 40.0 31.0 41.0 48-49 37.19675088708426 39.0 36.0 41.0 31.0 41.0 50-51 36.78841818798548 38.5 35.5 40.5 30.5 41.0 52-53 36.80984803831251 39.0 35.0 40.0 31.0 41.0 54-55 36.98637311690513 39.0 35.0 40.5 31.0 41.0 56-57 36.730938810418714 39.0 35.0 40.5 31.0 41.0 58-59 36.46644376744088 38.0 35.0 40.0 31.0 41.0 60-61 36.07568883348386 38.0 35.0 40.0 30.0 41.0 62-63 35.668827478709574 37.0 34.0 40.0 29.0 41.0 64-65 35.65212410172538 37.0 35.0 40.0 30.0 41.0 66-67 35.54900153229244 36.0 35.0 39.0 30.0 41.0 68-69 35.345832031783644 36.0 35.0 39.0 30.0 41.0 70-71 35.06148826469866 35.5 35.0 39.0 30.0 40.5 72-73 34.764363359457406 35.0 34.5 37.0 30.0 39.5 74-75 34.444457684280955 35.0 34.0 37.0 30.0 39.0 76-77 34.16399926547425 35.0 34.0 36.5 30.0 39.0 78-79 33.882308880260254 35.0 34.0 36.0 30.0 38.0 80-81 33.647351429642264 35.0 34.0 36.0 30.0 37.0 82-83 33.42046637046544 35.0 34.0 35.0 29.5 37.0 84-85 33.24725716367933 35.0 34.0 35.0 29.0 36.0 86-87 33.08676150800354 35.0 34.0 35.0 29.0 36.0 88-89 32.93139538401351 35.0 34.0 35.0 29.0 36.0 90-91 32.815130504939226 35.0 34.0 35.0 29.0 35.5 92-93 32.70799353874685 35.0 34.0 35.0 29.0 35.0 94-95 32.58399122620902 35.0 34.0 35.0 29.0 35.0 96-97 32.41697531988008 35.0 34.0 35.0 29.0 35.0 98-99 32.26475026535188 35.0 34.0 35.0 29.0 35.0 100-101 31.691687847363195 34.5 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09301013895927568 1101 2 0.2010279937353232 1101 3 0.31972383282925776 1101 4 0.02479099365483961 1101 5 -0.21829891059405782 1101 6 -0.038604412535541144 1101 7 0.10461086171321199 1101 8 0.23410341140404256 1101 9 0.43965819165507014 1101 10-11 0.5386077458319107 1101 12-13 0.6010454105172798 1101 14-15 0.6641548469358654 1101 16-17 0.7805836240067094 1101 18-19 0.7379632135696284 1101 20-21 0.8228236543458891 1101 22-23 1.103608856306714 1101 24-25 0.678819371382275 1101 26-27 0.7602628355325081 1101 28-29 0.758987820366471 1101 30-31 0.7845212991943455 1101 32-33 0.5996140373193626 1101 34-35 0.5698706333104795 1101 36-37 0.5638296544171766 1101 38-39 0.5663352471955392 1101 40-41 0.5693332831555082 1101 42-43 0.6543232236469052 1101 44-45 0.680261005426054 1101 46-47 0.5764727463724242 1101 48-49 0.5463579199423947 1101 50-51 0.521818199634204 1101 52-53 0.3724519793600507 1101 54-55 0.3044321424176957 1101 56-57 0.37828797098344324 1101 58-59 0.37988880945300707 1101 60-61 0.3950835967946915 1101 62-63 0.47523803734254955 1101 64-65 0.49874463806487057 1101 66-67 0.5106187109297906 1101 68-69 0.3239186430972296 1101 70-71 0.3860861507692803 1101 72-73 0.39123850857795617 1101 74-75 0.3535904026982095 1101 76-77 0.42465515065039483 1101 78-79 0.4387489320996778 1101 80-81 0.4024218693501922 1101 82-83 0.41805339503951444 1101 84-85 0.399799895424394 1101 86-87 0.3948465632727576 1101 88-89 0.43008213422361763 1101 90-91 0.3912365089461787 1101 92-93 0.3232094076120049 1101 94-95 0.3648337757312845 1101 96-97 0.41669467142583727 1101 98-99 0.40605132849928083 1101 100-101 0.2938639127326468 1102 1 0.2343465813691772 1102 2 0.18116603044558843 1102 3 0.1798471432447677 1102 4 0.021975218565067678 1102 5 -0.0668936595734948 1102 6 0.19403865874809156 1102 7 0.2540121261806476 1102 8 0.38764461375929926 1102 9 0.4422880913493046 1102 10-11 0.4781981779723452 1102 12-13 0.5596934734302366 1102 14-15 0.6001354916185804 1102 16-17 0.6955008984102307 1102 18-19 0.6098461497576153 1102 20-21 0.6432303934264354 1102 22-23 0.9474917431043792 1102 24-25 0.6390202534390568 1102 26-27 0.6235617505514739 1102 28-29 0.5873424626864505 1102 30-31 0.6138380902367118 1102 32-33 0.5402236249735282 1102 34-35 0.42815974371691823 1102 36-37 0.4178069314109649 1102 38-39 0.42024845188141313 1102 40-41 0.40650109890907515 1102 42-43 0.5203259720261073 1102 44-45 0.5622020866450654 1102 46-47 0.45192766416746366 1102 48-49 0.4280670603482335 1102 50-51 0.37113263349773007 1102 52-53 0.16561982016944654 1102 54-55 0.3135842955606378 1102 56-57 0.4354217559950868 1102 58-59 0.4107247809648271 1102 60-61 0.5383928186765417 1102 62-63 0.5849928280634344 1102 64-65 0.5376095544302473 1102 66-67 0.43141154279888383 1102 68-69 0.345653193028582 1102 70-71 0.31002406238045666 1102 72-73 0.2279501002744979 1102 74-75 0.27053426110875733 1102 76-77 0.2631442637461987 1102 78-79 0.2352894979325768 1102 80-81 0.2745542924614739 1102 82-83 0.31191081368981344 1102 84-85 0.2861680216671374 1102 86-87 0.31230069823481443 1102 88-89 0.29406685186866355 1102 90-91 0.12775077124725698 1102 92-93 0.09119290649555722 1102 94-95 0.12572821783021482 1102 96-97 0.15708522316065654 1102 98-99 0.21499331855839188 1102 100-101 0.2877672550526711 1103 1 0.24278296339168648 1103 2 0.19458771579217427 1103 3 0.2508727668913373 1103 4 -0.09799504395078174 1103 5 0.1104223420202004 1103 6 0.23588865815044358 1103 7 0.27826400324676115 1103 8 0.36878026859815805 1103 9 0.307715081542554 1103 10-11 0.383543444584447 1103 12-13 0.4158158588881449 1103 14-15 0.4289951470825031 1103 16-17 0.5811911479897134 1103 18-19 0.5734544368318026 1103 20-21 0.6108667241348442 1103 22-23 0.8538449495931388 1103 24-25 0.527170846046431 1103 26-27 0.41129913573809773 1103 28-29 0.45191423481675486 1103 30-31 0.47076808011419047 1103 32-33 0.37845543646839275 1103 34-35 0.2877819322426163 1103 36-37 0.2625701834521905 1103 38-39 0.3171913226387417 1103 40-41 0.36659236244598503 1103 42-43 0.47951117166812907 1103 44-45 0.3906304714162516 1103 46-47 0.19697337624069178 1103 48-49 0.2644680875910055 1103 50-51 0.3260788901044904 1103 52-53 0.20542367614897472 1103 54-55 0.2707160549605305 1103 56-57 0.33819918672119087 1103 58-59 0.34426011512143617 1103 60-61 0.38391635363176846 1103 62-63 0.4204802657005331 1103 64-65 0.4078414579732197 1103 66-67 0.269921820049035 1103 68-69 0.18893288798168584 1103 70-71 0.20370189020265883 1103 72-73 0.27398136507195403 1103 74-75 0.2095259313455884 1103 76-77 0.2975466368853077 1103 78-79 0.2749531970357211 1103 80-81 0.29277764451290267 1103 82-83 0.3114901882577428 1103 84-85 0.29959408125029086 1103 86-87 0.34888831214654203 1103 88-89 0.3563185773507911 1103 90-91 0.33757293972544034 1103 92-93 0.3025352149431413 1103 94-95 0.3052501110263961 1103 96-97 0.3572777923299739 1103 98-99 0.3246390172503766 1103 100-101 0.284120013347831 1104 1 0.21949499267497785 1104 2 0.25298443480081545 1104 3 0.1355548417153436 1104 4 0.06336796855213578 1104 5 0.21526445009686768 1104 6 0.30495081702672167 1104 7 0.2822529698139533 1104 8 0.39224256286143344 1104 9 0.31337334154021335 1104 10-11 0.23620687401557916 1104 12-13 0.3605370068652505 1104 14-15 0.3906213493023003 1104 16-17 0.47674902124859386 1104 18-19 0.48657891983108215 1104 20-21 0.5965339326525836 1104 22-23 0.8951248371805249 1104 24-25 0.48129224121431946 1104 26-27 0.4493234679294176 1104 28-29 0.42952658879455896 1104 30-31 0.45322265898199277 1104 32-33 0.41605337650671714 1104 34-35 0.3183113796369099 1104 36-37 0.16938626117665478 1104 38-39 0.11308659486443418 1104 40-41 0.2503219495201279 1104 42-43 0.34437212000386097 1104 44-45 0.2824351312510558 1104 46-47 0.2653298920447682 1104 48-49 0.28093795429040114 1104 50-51 0.3295713077398119 1104 52-53 0.3547906595576791 1104 54-55 0.3267102212592903 1104 56-57 0.21733408712631075 1104 58-59 0.26871327117307686 1104 60-61 0.3744512349207767 1104 62-63 0.2785853093912749 1104 64-65 0.31707923249592085 1104 66-67 0.2429686224822305 1104 68-69 0.25398299561536675 1104 70-71 0.26338890357097 1104 72-73 0.3355311085541217 1104 74-75 0.34902134813275865 1104 76-77 0.34828024901205623 1104 78-79 0.2926041061069711 1104 80-81 0.3417021546197532 1104 82-83 0.2414282884044141 1104 84-85 0.26369698513175877 1104 86-87 0.3271495870969261 1104 88-89 0.27925707949810175 1104 90-91 0.3224914544724058 1104 92-93 0.3233340953656736 1104 94-95 0.42330414771252833 1104 96-97 0.27573547787751096 1104 98-99 0.28076723389484926 1104 100-101 0.3126208683102121 1105 1 0.12279237477233451 1105 2 0.05975172498394343 1105 3 0.14235554580764997 1105 4 0.017247507758121117 1105 5 0.2245056145591704 1105 6 0.23530981724285027 1105 7 0.1268282976660302 1105 8 0.1158717639815805 1105 9 0.06221029952744317 1105 10-11 -0.007686528960903161 1105 12-13 0.12526728059621917 1105 14-15 0.061982622040368085 1105 16-17 0.18634123387839452 1105 18-19 0.06533632859646588 1105 20-21 0.22782937146198634 1105 22-23 0.46209759054917754 1105 24-25 0.22041523011726838 1105 26-27 0.145031448667865 1105 28-29 0.1342997950150533 1105 30-31 0.047184341862894996 1105 32-33 0.02415811801704848 1105 34-35 -0.013928781731621598 1105 36-37 -0.15848709218821 1105 38-39 -0.0865436520762799 1105 40-41 -0.05428694785577193 1105 42-43 -0.04158433663744887 1105 44-45 -0.10590211141559536 1105 46-47 -0.06060978602032918 1105 48-49 -0.04788718263222336 1105 50-51 -0.08398671846854455 1105 52-53 -0.05977034767227707 1105 54-55 -0.025784000223282533 1105 56-57 0.05552122155979333 1105 58-59 0.029693034064656842 1105 60-61 -0.025345722940507187 1105 62-63 0.009791733607869446 1105 64-65 -0.059054361536269084 1105 66-67 -0.13686222337292264 1105 68-69 -0.12307142713663666 1105 70-71 -0.025730067424952097 1105 72-73 -0.06582001946259197 1105 74-75 -0.002091424166550837 1105 76-77 -0.05486044681331492 1105 78-79 -0.040078740320915074 1105 80-81 0.004204049401735688 1105 82-83 0.011998651508257296 1105 84-85 0.028346489549313958 1105 86-87 0.06841060619120043 1105 88-89 -0.027711123621607214 1105 90-91 -0.02295757498658446 1105 92-93 -0.043339603267568805 1105 94-95 -0.06758280250917892 1105 96-97 -0.06307437422046291 1105 98-99 -0.042342853594774965 1105 100-101 -0.04321200604235287 1106 1 -0.07114980295040851 1106 2 -0.1386757287220739 1106 3 -0.05284185562966215 1106 4 0.2589911619462981 1106 5 0.10999476242703565 1106 6 0.11126735379332331 1106 7 0.006513691334554039 1106 8 -0.12410233677050542 1106 9 -0.16971029120455228 1106 10-11 -0.2982967275665942 1106 12-13 -0.24417851990317985 1106 14-15 -0.29446678555967054 1106 16-17 -0.2544045578530145 1106 18-19 -0.41598477222600394 1106 20-21 -0.08952894631767805 1106 22-23 0.06400971894542096 1106 24-25 -0.5338000258448687 1106 26-27 -0.7855168850124201 1106 28-29 -0.8235664049445717 1106 30-31 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94-95 -0.21525604761063732 1106 96-97 -0.26907629982644465 1106 98-99 -0.26210318190258164 1106 100-101 -0.26809906291040164 1107 1 -0.14651844908834377 1107 2 -0.2906464222433449 1107 3 -0.18635421245713601 1107 4 0.19324619964221768 1107 5 0.037941141846211224 1107 6 -0.04129891414629583 1107 7 -0.23637985834032804 1107 8 -0.39020955222059683 1107 9 -0.47353576933171126 1107 10-11 -0.7457140752378564 1107 12-13 -0.6717569209006129 1107 14-15 -0.5612433615561514 1107 16-17 -0.5062689292878986 1107 18-19 -0.6564749599256388 1107 20-21 -0.8413671587496552 1107 22-23 -2.652816403652494 1107 24-25 -1.3707921956977245 1107 26-27 -0.9122208051458784 1107 28-29 -0.7082545085168803 1107 30-31 -0.8770640833149557 1107 32-33 -0.8120110088843262 1107 34-35 -0.8284893359586647 1107 36-37 -0.7136117228250072 1107 38-39 -0.8191804319989515 1107 40-41 -0.7815640442775873 1107 42-43 -0.9941172813605945 1107 44-45 -0.9420807619930329 1107 46-47 -0.8478308194995421 1107 48-49 -0.9571407165617174 1107 50-51 -0.7864662621889309 1107 52-53 -0.6699226687635687 1107 54-55 -0.7202657404950585 1107 56-57 -0.8009215168145047 1107 58-59 -0.6728806523593462 1107 60-61 -0.9882870700846951 1107 62-63 -1.0316239303959165 1107 64-65 -0.9250562757308529 1107 66-67 -0.7662949048975491 1107 68-69 -0.7138809246232185 1107 70-71 -0.5357440406625429 1107 72-73 -0.5168101505369904 1107 74-75 -0.563676956710232 1107 76-77 -0.5336042755250432 1107 78-79 -0.4762675140528714 1107 80-81 -0.3983795490644866 1107 82-83 -0.4852486535285223 1107 84-85 -0.4578162696869157 1107 86-87 -0.4532687171030929 1107 88-89 -0.4676591216469106 1107 90-91 -0.5741689498353466 1107 92-93 -0.6251458134756689 1107 94-95 -0.4461027185775066 1107 96-97 -0.419532757040443 1107 98-99 -0.4489841194291486 1107 100-101 -0.4971704505380963 1108 1 -0.968239017473902 1108 2 -1.1178634796054574 1108 3 -1.2034579372031473 1108 4 -1.224755006507209 1108 5 -0.7040976914401398 1108 6 -1.6093900645129011 1108 7 -2.7145011198371023 1108 8 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-0.03820687626146935 2305 36-37 -0.06958816522048039 2305 38-39 -0.1067661016795256 2305 40-41 0.0019184080043288532 2305 42-43 -0.004904923418145302 2305 44-45 -0.03811660419497542 2305 46-47 -0.06196249792736097 2305 48-49 -0.08161506545705066 2305 50-51 -0.0947959701782608 2305 52-53 -0.05072427560811121 2305 54-55 -0.013273724541747356 2305 56-57 -0.1212870303449165 2305 58-59 -0.08976972351965884 2305 60-61 -0.1597943298829918 2305 62-63 -0.08674992093273914 2305 64-65 -0.246094702840864 2305 66-67 -0.17306435380062624 2305 68-69 -0.08375376112599042 2305 70-71 -0.14554880556350014 2305 72-73 -0.17959043439297062 2305 74-75 -0.11318941768916346 2305 76-77 -0.13721666634480556 2305 78-79 -0.2089411349147028 2305 80-81 -0.10672193184980472 2305 82-83 -0.06473838283806543 2305 84-85 -0.07742484787959825 2305 86-87 -0.12047285600602464 2305 88-89 0.021511349238316768 2305 90-91 -0.03724885560468749 2305 92-93 -0.06864587363082819 2305 94-95 -0.14098764832446875 2305 96-97 -0.2026246058657435 2305 98-99 -0.19729312686139622 2305 100-101 -0.19435666060049428 2306 1 -0.09058301468563101 2306 2 -0.3081999722920088 2306 3 -0.21071892524108193 2306 4 0.2755069430460324 2306 5 0.24793212658702402 2306 6 0.14109226545328113 2306 7 0.0038794563637978285 2306 8 -0.1535516894550284 2306 9 -0.27926684977191485 2306 10-11 -0.4080262118912117 2306 12-13 -0.19966117192451804 2306 14-15 -0.26382316034824527 2306 16-17 -0.1602680458577197 2306 18-19 -0.25369906275467 2306 20-21 -0.16167246832090854 2306 22-23 0.015676921211465356 2306 24-25 -0.14225014918066847 2306 26-27 -0.3607127436256192 2306 28-29 -0.4289957170599479 2306 30-31 -0.4053144169942229 2306 32-33 -0.45063269918015436 2306 34-35 -0.3508002994097623 2306 36-37 -0.34442577861274515 2306 38-39 -0.3991580454555148 2306 40-41 -0.3314396065427019 2306 42-43 -0.20737500298465505 2306 44-45 -0.35584614402785775 2306 46-47 -0.2880091762675505 2306 48-49 -0.38867236280061945 2306 50-51 -0.27646303632112534 2306 52-53 -0.2575460126058857 2306 54-55 -0.33432208302264854 2306 56-57 -0.3053403999481361 2306 58-59 -0.3468431221681314 2306 60-61 -0.41490352398200514 2306 62-63 -0.38756947515825857 2306 64-65 -0.48051840663671186 2306 66-67 -0.4248055026649382 2306 68-69 -0.38387659875334634 2306 70-71 -0.2791359687743906 2306 72-73 -0.27940862935445665 2306 74-75 -0.31099054725280695 2306 76-77 -0.2904477454724699 2306 78-79 -0.35261697933561464 2306 80-81 -0.46209474262771977 2306 82-83 -0.6342035856600674 2306 84-85 -0.4988795498378451 2306 86-87 -0.3910512881487378 2306 88-89 -0.30103317981956934 2306 90-91 -0.23735349893512847 2306 92-93 -0.31010460107204807 2306 94-95 -0.31875054771211353 2306 96-97 -0.3621273987404763 2306 98-99 -0.3624580221337368 2306 100-101 -0.39820997486011933 2307 1 -0.1301318701092491 2307 2 -0.12842827030270243 2307 3 0.043584938856252364 2307 4 0.4398439747533942 2307 5 0.16795542868754154 2307 6 0.06401240032602828 2307 7 -0.001925171037896689 2307 8 -0.24737794338066266 2307 9 -0.4814489186930828 2307 10-11 -0.38632095157618096 2307 12-13 -0.4354574115614511 2307 14-15 -0.55486055093548 2307 16-17 -0.4133042197652017 2307 18-19 -0.4178145353475671 2307 20-21 -0.4856131086327977 2307 22-23 -1.6656020779971001 2307 24-25 -0.688048964206061 2307 26-27 -0.5940675549092234 2307 28-29 -0.6872339429169401 2307 30-31 -0.5164384964864368 2307 32-33 -0.5032336935653206 2307 34-35 -0.597252233769062 2307 36-37 -0.49284821588859984 2307 38-39 -0.5227393145077812 2307 40-41 -0.5675243104227405 2307 42-43 -0.5718383314871573 2307 44-45 -0.6573081865965023 2307 46-47 -0.6493094649148432 2307 48-49 -0.598138692503035 2307 50-51 -0.629402704305484 2307 52-53 -0.5428522688342667 2307 54-55 -0.5071264164035654 2307 56-57 -0.7346908952731539 2307 58-59 -0.6486814183365368 2307 60-61 -0.7029938300514829 2307 62-63 -0.7230254830462997 2307 64-65 -0.9084982013634573 2307 66-67 -0.7399092296170195 2307 68-69 -0.5723812816560496 2307 70-71 -0.5196561100881709 2307 72-73 -0.4343991225892694 2307 74-75 -0.48710268990348027 2307 76-77 -0.5668296702295592 2307 78-79 -0.4645408802111177 2307 80-81 -0.5349403217514634 2307 82-83 -0.39159245117895836 2307 84-85 -0.4484234844675541 2307 86-87 -0.5197990371138062 2307 88-89 -0.41885988421813636 2307 90-91 -0.4439350809079272 2307 92-93 -0.5025475215339412 2307 94-95 -0.4571371184930939 2307 96-97 -0.47811206022943864 2307 98-99 -0.5093808206405939 2307 100-101 -0.6318058606040218 2308 1 -0.5261201719438908 2308 2 -0.3769303671784847 2308 3 -0.494017913232053 2308 4 -0.2511667773030979 2308 5 -0.2631423633637837 2308 6 -0.3949343179824609 2308 7 -0.5414453468647125 2308 8 -0.6996673741313089 2308 9 -1.1180024458771385 2308 10-11 -1.2523816423362746 2308 12-13 -2.0581357291482547 2308 14-15 -1.495392394583483 2308 16-17 -1.4144293833508073 2308 18-19 -1.1190681072475144 2308 20-21 -2.879531193656554 2308 22-23 -4.649550926420098 2308 24-25 -2.253670819130363 2308 26-27 -1.4317049096073404 2308 28-29 -1.4641541194353422 2308 30-31 -1.8474831442692405 2308 32-33 -1.1660740191330703 2308 34-35 -1.0166027596227423 2308 36-37 -0.7776352430969098 2308 38-39 -1.1482529487364488 2308 40-41 -1.7605744692673113 2308 42-43 -1.80242870209522 2308 44-45 -1.7063516907807568 2308 46-47 -0.9133125940559736 2308 48-49 -0.8446758998823185 2308 50-51 -0.8786462512046995 2308 52-53 -0.8019654084334391 2308 54-55 -0.9898845889040331 2308 56-57 -1.2549651584429498 2308 58-59 -1.1259412977065821 2308 60-61 -1.0487858781135486 2308 62-63 -0.9866944427768516 2308 64-65 -1.0753197161843318 2308 66-67 -0.8593248256607495 2308 68-69 -0.8128398932689862 2308 70-71 -0.6782619828789436 2308 72-73 -0.7672431135469395 2308 74-75 -0.8523707659308357 2308 76-77 -0.9074758053509626 2308 78-79 -0.8128229204260506 2308 80-81 -0.5992844860128343 2308 82-83 -0.6612605018275488 2308 84-85 -0.6866674301148805 2308 86-87 -0.7750280208290903 2308 88-89 -0.6426734341397236 2308 90-91 -0.5440970041893749 2308 92-93 -0.6313434331749619 2308 94-95 -0.7117327089210121 2308 96-97 -0.8153647564609656 2308 98-99 -0.7763385419569673 2308 100-101 -0.869747952416482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 261.0 11 2088.0 12 5832.0 13 10462.0 14 15609.0 15 19458.0 16 22100.0 17 24738.0 18 27340.0 19 31782.0 20 38700.0 21 45746.0 22 54265.0 23 66509.0 24 80339.0 25 96035.0 26 113588.0 27 136210.0 28 163453.0 29 197605.0 30 242764.0 31 303232.0 32 391480.0 33 532953.0 34 795575.0 35 1299933.0 36 2391837.0 37 3981060.0 38 3106889.0 39 412700.0 40 244.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.853326459873347 13.530871846200219 13.590191028711113 41.02561066521532 2 25.398541132757522 18.026173536978295 28.586404785286053 27.988880544978134 3 30.80116554972942 17.34866729980614 20.088690630512193 31.761476519952243 4 31.061261035339093 21.51634805733955 18.926330325236393 28.496060582084965 5 31.439866959874756 24.507100421900148 21.55130113770881 22.501731480516284 6 28.424360137180827 28.89965277711236 19.869167905249192 22.80681918045762 7 23.5597425667688 22.055994904351156 32.81174907434614 21.572513454533905 8 23.31973167367488 23.717296009497684 26.941237722967326 26.021734593860106 9 23.01320410778644 22.182434221960907 29.245898398419467 25.558463271833183 10-11 25.621422106329184 28.022958623109663 22.651416959871458 23.704202310689695 12-13 25.113698021695836 23.902064494463993 25.532263762298218 25.451973721541954 14-15 25.150009498311537 24.5852885921052 25.38328022708887 24.88142168249439 16-17 25.760927473590062 24.403824078482284 24.718543585739525 25.116704862188133 18-19 25.469584528911106 24.736985438430835 24.632189584846483 25.16124044781158 20-21 25.522594894484495 24.77322289879707 24.7079114769296 24.996270729788836 22-23 25.830230571692958 24.61300121007704 24.642880120241138 24.913888097988863 24-25 25.415288304194593 24.736833495638567 24.781770872831636 25.066107327335203 26-27 25.360364030681115 24.875689958757413 24.704914974741364 25.059031035820105 28-29 25.500992373624864 24.66174649750133 24.522187593435525 25.31507353543828 30-31 25.373943789675103 24.66793794214555 24.57241604156053 25.38570222661882 32-33 25.279838666624688 24.815635621467496 24.684054332634762 25.220471379273057 34-35 25.44230128217852 24.69154958594251 24.56311955477954 25.30302957709943 36-37 25.351883827210415 24.640905747178042 24.60061360140192 25.406596824209622 38-39 25.332302766845842 24.802274656349415 24.56471644187361 25.300706134931133 40-41 25.437489312261825 24.68019600999549 24.488543069775407 25.39377160796728 42-43 25.372462926867094 24.624210762694922 24.641855265388582 25.3614710450494 44-45 25.43868954911946 24.65247254289091 24.674774556991725 25.234063350997904 46-47 25.458130663452238 24.58863272809105 24.517021258538556 25.436215349918157 48-49 25.367217770086654 24.573635826455106 24.565012055996878 25.49413434746136 50-51 25.35696292185073 24.752407718030657 24.557461114543592 25.33316824557502 52-53 25.406860383828768 24.74379201776003 24.50611144397023 25.343236154440973 54-55 25.277422939479248 24.64755339981783 24.671973818682773 25.40304984202015 56-57 25.344686318492972 24.68522748280615 24.648010620990803 25.322075577710073 58-59 25.510698858634417 24.596911699660268 24.47004311027041 25.42234633143491 60-61 25.314956036899922 24.706396769745904 24.511923503737023 25.466723689617147 62-63 25.236408324529997 24.889589984175732 24.565544463588353 25.308457227705915 64-65 25.398465237354 24.73933555218619 24.503270871558808 25.358928338901 66-67 25.378743727985796 24.624004251120255 24.56987624922685 25.427375771667098 68-69 25.372796456974122 24.705231504283 24.553531951870085 25.368440086872795 70-71 25.507667087744117 24.655788602395074 24.440628780555564 25.395915529305242 72-73 25.36721489434371 24.640406540402502 24.535978852999737 25.456399712254047 74-75 25.362998901907275 24.699208557402926 24.606410687773995 25.3313818529158 76-77 25.515171560730476 24.587967298494913 24.498111564908935 25.398749575865676 78-79 25.47311904532051 24.564211407571786 24.518939870576585 25.443729676531117 80-81 25.476537231004766 24.65547583703985 24.49103358344006 25.37695334851532 82-83 25.519779914042186 24.619632042505966 24.427470910783946 25.433117132667903 84-85 25.40027528759464 24.598258305126812 24.550097862580493 25.45136854469806 86-87 25.4317357831761 24.675780662891007 24.553922074565726 25.33856147936716 88-89 25.627437069102637 24.538577248537177 24.431019736923158 25.40296594543703 90-91 25.476791532393733 24.584368755470273 24.45993672620532 25.478902985930667 92-93 25.43698706989569 24.69580180725378 24.485481172232543 25.38172995061799 94-95 25.51139697295577 24.68471668621558 24.4078630258636 25.396023314965056 96-97 25.425118549714227 24.629710949774783 24.4819635517634 25.46320694874759 98-99 25.50245023766958 24.70383486432621 24.416070235526636 25.37764466247758 100-101 25.699909070074423 24.5629958287766 24.32517015011223 25.411924951036745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 62.0 1 42.5 2 22.0 3 18.5 4 20.0 5 26.0 6 33.0 7 38.5 8 44.5 9 56.0 10 80.5 11 110.0 12 139.5 13 191.5 14 241.5 15 350.0 16 486.0 17 652.5 18 968.0 19 1528.5 20 2402.0 21 3637.5 22 5465.5 23 8106.0 24 11398.0 25 14901.0 26 18934.5 27 24465.0 28 31192.5 29 39830.0 30 51111.5 31 62868.5 32 75730.5 33 91296.5 34 109067.5 35 129337.5 36 154763.0 37 183077.5 38 213085.0 39 246617.5 40 279702.5 41 311156.5 42 345064.0 43 382702.0 44 419887.5 45 458469.0 46 499016.0 47 535800.0 48 571587.0 49 601167.5 50 616248.5 51 627589.5 52 640986.5 53 646469.0 54 643678.0 55 633676.5 56 609392.5 57 573502.5 58 532344.5 59 486682.0 60 434500.5 61 377382.0 62 320133.0 63 263642.0 64 211559.0 65 168590.5 66 135449.0 67 112218.0 68 93306.5 69 78887.0 70 68384.0 71 58169.0 72 50912.0 73 45644.0 74 42017.0 75 39069.5 76 35705.0 77 32120.5 78 27580.0 79 23284.5 80 20231.5 81 18231.0 82 16005.5 83 13054.0 84 9846.5 85 7138.0 86 4998.5 87 2906.5 88 1342.0 89 563.5 90 240.5 91 98.0 92 39.5 93 21.5 94 15.5 95 4.5 96 3.0 97 3.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.003141514338583244 6 0.0 7 0.026117687834495994 8 4.8594230509675456E-4 9 0.01732281653802656 10-11 0.018356299468584445 12-13 0.008589543561921506 14-15 0.00202932244311532 16-17 0.0 18-19 0.0 20-21 1.4030728527441504E-4 22-23 0.0010129501571030939 24-25 0.0031209815651284515 26-27 2.8403669945796213E-4 28-29 7.631347467364526E-4 30-31 1.7110644545660372E-5 32-33 3.285243752766791E-4 34-35 0.003408440393495546 36-37 0.0 38-39 3.6616779327713195E-4 40-41 1.7110644545660372E-5 42-43 3.4221289091320743E-5 44-45 0.0 46-47 0.0 48-49 6.502044927350941E-5 50-51 0.0025802851974855837 52-53 1.3688515636528296E-5 54-55 4.3803250036890547E-4 56-57 0.0032510224636754705 58-59 3.422128909132074E-6 60-61 0.00202932244311532 62-63 0.008668252526831543 64-65 0.002638461388940829 66-67 3.490571487314716E-4 68-69 0.0 70-71 0.0017350193569299615 72-73 0.004640406800783092 74-75 0.0 76-77 9.958395125574337E-4 78-79 4.277661136415093E-4 80-81 0.003367374846585961 82-83 9.102862898291317E-4 84-85 0.001707642325656905 86-87 0.0 88-89 0.0 90-91 0.0 92-93 6.844257818264148E-6 94-95 3.422128909132074E-6 96-97 2.7377031273056595E-4 98-99 4.277661136415093E-4 100-101 5.920283012798488E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4610788E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.91667655757689 #Duplication Level Percentage of deduplicated Percentage of total 1 81.074413370289 53.44155883224627 2 11.496736486302925 15.15653320870646 3 3.2269601762998734 6.381314706160202 4 1.4052296100810326 3.705122627873653 5 0.7168457079512204 2.362604333635391 6 0.4530007813037988 1.7916183588919277 7 0.30710729787973456 1.417044469596692 8 0.22312275708223564 1.1766008488979618 9 0.1580260353002829 0.9374895950908478 >10 0.8804678172892421 10.506252852475226 >50 0.04752374836404554 2.09377499064376 >100 0.010534397331837008 1.0179396221596304 >500 3.181452482628385E-5 0.012145553621941632 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0020395888298427164 0.0 0.0 6.844257818264148E-6 2.7377031273056592E-5 2 0.0020395888298427164 0.0 0.0 6.844257818264148E-6 5.4754062546113184E-5 3 0.002114875665843622 0.0 0.0 1.3688515636528296E-5 1.1635238291049052E-4 4 0.0021285641814801502 6.844257818264148E-6 0.0 1.3688515636528296E-5 1.6426218763833955E-4 5 0.002135408439298414 6.844257818264148E-6 0.0 1.3688515636528296E-5 1.6426218763833955E-4 6 0.0021559412127532067 6.844257818264148E-6 0.0 1.3688515636528296E-5 1.984834767296603E-4 7 0.0021833182440262632 1.3688515636528296E-5 0.0 1.3688515636528296E-5 2.121719923661886E-4 8 0.0021970067596627915 1.3688515636528296E-5 0.0 2.0532773454792444E-5 2.3270476582098104E-4 9 0.0021970067596627915 2.0532773454792444E-5 0.0 3.4221289091320743E-5 2.8061457054883007E-4 10-11 0.0022038510174810554 2.0532773454792444E-5 0.0 4.4487675818716964E-5 3.1141373073101874E-4 12-13 0.0022414944354815086 2.0532773454792444E-5 0.0 5.4754062546113184E-5 3.5247927764060365E-4 14-15 0.0022791378534819614 2.0532773454792444E-5 0.0 5.4754062546113184E-5 4.1065546909584887E-4 16-17 0.0023646910762102633 2.3954902363924516E-5 0.0 5.4754062546113184E-5 4.482988870963017E-4 18-19 0.0026487277756682254 5.4754062546113184E-5 0.0 6.159832036437734E-5 4.7225378946022626E-4 20-21 0.004243439847323772 6.159832036437734E-5 0.0 6.502044927350941E-5 4.8594230509675456E-4 22-23 0.005341943227155168 6.502044927350941E-5 0.0 7.186470709177356E-5 4.8594230509675456E-4 24-25 0.006628663696988828 6.844257818264149E-5 0.0 7.528683600090564E-5 4.962086918241507E-4 26-27 0.008500568210284071 7.186470709177356E-5 0.0 7.87089649100377E-5 5.783397856433206E-4 28-29 0.010358784207942787 8.213109381916979E-5 0.0 8.897535163743393E-5 6.194053325529054E-4 30-31 0.012186201045419315 8.897535163743393E-5 0.0 1.0266386727396223E-4 6.4336023491683E-4 32-33 0.014153925168170258 8.897535163743393E-5 0.0 1.0950812509222637E-4 6.673151372807545E-4 34-35 0.01644675153728875 1.0266386727396222E-4 0.0 1.0950812509222637E-4 6.946921685538111E-4 36-37 0.01834603308185705 1.1293025400135844E-4 0.0 1.0950812509222637E-4 7.460241021907921E-4 38-39 0.020016031989513502 1.197745118196226E-4 0.0 1.1635238291049052E-4 7.905117780095091E-4 40-41 0.022319124745359388 1.2319664072875467E-4 0.0 1.1635238291049052E-4 8.349994538282261E-4 42-43 0.02431764802829252 1.2319664072875467E-4 0.0 1.2319664072875467E-4 8.931756452834714E-4 44-45 0.026853445549959387 1.3004089854701882E-4 0.0 1.2319664072875467E-4 9.308190632839242E-4 46-47 0.028841702446165123 1.3688515636528297E-4 0.0 1.2319664072875467E-4 9.513518367387166E-4 48-49 0.030823115084552592 1.3688515636528297E-4 0.0 1.3004089854701882E-4 9.753067391026412E-4 50-51 0.03285928178548618 1.3688515636528297E-4 0.0 1.334630274561509E-4 0.0010369050594670184 52-53 0.03662020145662233 1.3688515636528297E-4 0.0 1.4030728527441504E-4 0.0010950812509222638 54-55 0.04120927632376843 1.3688515636528297E-4 0.0 1.5399580091094334E-4 0.0011224582821953203 56-57 0.04504206070199636 1.5057367200181128E-4 0.0 1.7452857436573577E-4 0.001160101700195773 58-59 0.04871058289258594 1.6426218763833955E-4 0.0 1.9163921891139616E-4 0.0011772123447414335 60-61 0.05265971965372436 1.6426218763833955E-4 0.0 1.984834767296603E-4 0.0011977451181962259 62-63 0.056961335692503376 1.6426218763833955E-4 0.0 1.984834767296603E-4 0.0012114336338327543 64-65 0.061796803841106994 1.6426218763833955E-4 0.0 1.984834767296603E-4 0.0012353885361966787 66-67 0.06714559132608042 1.711064454566037E-4 0.0 1.984834767296603E-4 0.0012593434385606032 68-69 0.07290161215124058 1.7452857436573577E-4 0.0 1.984834767296603E-4 0.0013140975011067166 70-71 0.09010807630635664 1.9506134782052825E-4 0.0 2.0190560563879237E-4 0.001375695821471094 72-73 0.10654798358582712 2.0874986345705655E-4 0.0 2.0532773454792446E-4 0.0014236056261989428 74-75 0.11430252769392041 2.121719923661886E-4 0.0 2.1901625018445274E-4 0.0014612490441993958 76-77 0.12325481692020993 2.121719923661886E-4 0.0 2.258605080027169E-4 0.0014954703332907163 78-79 0.13302157282687285 2.224383790935848E-4 0.0 2.258605080027169E-4 0.0015125809778363769 80-81 0.14460547918428493 2.2928263691184898E-4 0.0 2.258605080027169E-4 0.0015125809778363769 82-83 0.15815368753553882 2.395490236392452E-4 0.0 2.258605080027169E-4 0.0015262694934729051 84-85 0.1751069141513791 2.4297115254837728E-4 0.0 2.2928263691184898E-4 0.0015399580091094334 86-87 0.1943974548121566 2.4639328145750934E-4 0.0 2.395490236392452E-4 0.0015604907825642258 88-89 0.21939952862227552 2.4639328145750934E-4 0.0 2.395490236392452E-4 0.0015673350403824899 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 520 2.4011206E-7 11.877377 1 CGGGGAT 6005 0.0 7.6743083 1 GCCGGGA 7725 0.0 7.318616 1 CCCGGAT 5865 0.0 7.2094555 1 CCCCGAT 3220 0.0 7.0821614 1 CGGCGAT 2225 0.0 7.046353 1 CGGGGTA 2240 0.0 6.999168 1 TCTAACG 1030 2.4383384E-5 6.919641 7 CCCCCGT 3240 0.0 6.8918104 1 CCGGGAT 5075 0.0 6.833879 1 CGGGGTT 4215 0.0 6.7629185 1 GCCGGGT 5885 0.0 6.700576 1 GTCGGTT 2625 0.0 6.6965775 1 CGGGATA 1655 9.587893E-9 6.602529 2 CCGGCAT 4470 0.0 6.589685 1 GCCCCGT 4545 0.0 6.5854754 1 TCGTGCG 1485 4.0495615E-7 6.399309 7 CTCCCGT 4790 0.0 6.1494555 1 GCGGGTT 2720 0.0 6.113355 1 CGGGGGA 6020 0.0 6.076797 1 >>END_MODULE