##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051302_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14610788 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.509827806686403 33.0 31.0 34.0 30.0 34.0 2 32.1460735724863 33.0 31.0 34.0 30.0 34.0 3 32.542443911991604 34.0 31.0 34.0 31.0 34.0 4 36.18265592519719 37.0 35.0 37.0 35.0 37.0 5 36.29538550555932 37.0 37.0 37.0 35.0 37.0 6 36.33879370503494 37.0 37.0 37.0 35.0 37.0 7 36.34992185226423 37.0 37.0 37.0 35.0 37.0 8 36.336637421609296 37.0 37.0 37.0 35.0 37.0 9 38.182450734347796 39.0 39.0 39.0 37.0 39.0 10-11 38.21074801030581 39.0 39.0 39.0 37.0 39.0 12-13 38.15139785752828 39.0 39.0 39.0 37.0 39.0 14-15 39.61930646724872 41.0 40.0 41.0 37.0 41.0 16-17 39.58904772966386 41.0 39.0 41.0 37.0 41.0 18-19 39.59764428859005 41.0 39.5 41.0 37.0 41.0 20-21 39.55921152233542 41.0 39.0 41.0 37.0 41.0 22-23 39.49649454225193 41.0 39.0 41.0 37.0 41.0 24-25 39.44933555260674 41.0 39.0 41.0 37.0 41.0 26-27 39.26773819454502 40.0 39.0 41.0 36.0 41.0 28-29 39.18359872171166 40.0 39.0 41.0 36.0 41.0 30-31 39.038919666755824 40.0 38.5 41.0 35.5 41.0 32-33 38.89241716463205 40.0 38.0 41.0 35.0 41.0 34-35 38.753281958509014 40.0 38.0 41.0 35.0 41.0 36-37 38.62676509987004 40.0 38.0 41.0 35.0 41.0 38-39 38.44310556692767 40.0 38.0 41.0 34.0 41.0 40-41 38.30519431943027 40.0 38.0 41.0 34.0 41.0 42-43 38.100167629562485 40.0 37.5 41.0 34.0 41.0 44-45 37.92862215918812 40.0 37.0 40.5 33.0 41.0 46-47 37.68490433233306 39.0 37.0 40.0 33.0 41.0 48-49 37.524269293346805 39.0 36.0 40.0 33.0 41.0 50-51 38.07491871759415 40.0 37.0 41.0 33.5 41.0 52-53 38.171823894782406 40.0 37.0 41.0 34.0 41.0 54-55 38.069764512358944 40.0 36.5 41.0 34.0 41.0 56-57 37.8226783866825 39.0 36.0 41.0 34.0 41.0 58-59 37.5372351238003 39.0 35.0 41.0 33.0 41.0 60-61 37.26174916096244 39.0 35.0 41.0 33.0 41.0 62-63 36.92942974738939 38.0 35.0 40.0 33.0 41.0 64-65 36.60187421102818 37.0 35.0 40.0 33.0 41.0 66-67 36.18916529348041 36.5 35.0 39.5 32.0 41.0 68-69 35.696239826352965 36.0 35.0 39.0 31.5 41.0 70-71 35.30541374633593 35.5 34.5 38.5 31.0 40.0 72-73 34.97714130818954 35.0 34.0 37.0 31.0 39.5 74-75 34.704467821995635 35.0 34.0 37.0 31.0 39.0 76-77 33.96468688068022 35.0 33.5 36.0 30.0 39.0 78-79 34.1660595239627 35.0 34.0 36.0 31.0 37.5 80-81 33.99608364723381 35.0 34.0 36.0 31.0 37.0 82-83 33.78833003394478 35.0 34.0 35.0 31.0 37.0 84-85 33.58155271981224 35.0 34.0 35.0 30.5 36.0 86-87 33.390795759954905 35.0 34.0 35.0 30.0 36.0 88-89 33.2707473067161 35.0 34.0 35.0 30.0 36.0 90-91 33.1241802632411 35.0 34.0 35.0 30.0 35.5 92-93 32.990965305909576 35.0 34.0 35.0 30.0 35.0 94-95 32.85710141027302 35.0 34.0 35.0 29.5 35.0 96-97 32.68776512943724 35.0 33.0 35.0 29.0 35.0 98-99 32.49069177514587 35.0 33.0 35.0 29.0 35.0 100-101 31.74624441884996 34.5 32.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03376626480440592 1101 2 -0.41393216417971956 1101 3 -0.3268098615332704 1101 4 -0.18944375857124385 1101 5 0.006049731790305657 1101 6 0.11598749157863608 1101 7 0.16303915278261627 1101 8 0.17952289700340174 1101 9 0.2334976556879056 1101 10-11 0.26809926498412295 1101 12-13 0.27600818140516736 1101 14-15 0.4066673080690535 1101 16-17 0.38390317964536536 1101 18-19 0.37437502640811005 1101 20-21 0.3202651550400333 1101 22-23 0.344479065222707 1101 24-25 0.3413550956430029 1101 26-27 0.37626175067021705 1101 28-29 0.3655729476387819 1101 30-31 0.36837772692290116 1101 32-33 0.3674365352807385 1101 34-35 0.33144627046179664 1101 36-37 0.31614982154506777 1101 38-39 0.3125726428514355 1101 40-41 0.29982442985091495 1101 42-43 0.1974305407431416 1101 44-45 0.12924464407228697 1101 46-47 0.1421572733915113 1101 48-49 0.09686801595332639 1101 50-51 0.24374124585629886 1101 52-53 0.30203714553685046 1101 54-55 0.3172722257888765 1101 56-57 0.33044454855364336 1101 58-59 0.32351031228315463 1101 60-61 0.2993930125701567 1101 62-63 0.26245759972655947 1101 64-65 0.25293922179469774 1101 66-67 0.3088557114124555 1101 68-69 0.32821221509284726 1101 70-71 0.31699829360139375 1101 72-73 0.2760166671284594 1101 74-75 0.23731636335853068 1101 76-77 0.1546076556010334 1101 78-79 0.1772930765320453 1101 80-81 0.14104471403388885 1101 82-83 0.11464942122449884 1101 84-85 0.1120769372821826 1101 86-87 0.11071877985734346 1101 88-89 0.09618455099938927 1101 90-91 0.09160355932700526 1101 92-93 0.05148223157003429 1101 94-95 0.060263002066889726 1101 96-97 0.0650682760569481 1101 98-99 0.06493886566298102 1101 100-101 -0.005054846146069991 1102 1 0.3185074781585513 1102 2 -0.02709645747260936 1102 3 0.018859562705650035 1102 4 0.010819529479839218 1102 5 0.06615159066903686 1102 6 0.11056188691915736 1102 7 0.15570426879613564 1102 8 0.16206314702090907 1102 9 0.14666561307799242 1102 10-11 0.18408938009772413 1102 12-13 0.212548823137503 1102 14-15 0.27928340712611543 1102 16-17 0.2954782837214438 1102 18-19 0.2642197726576683 1102 20-21 0.265441942452199 1102 22-23 0.3112262912423702 1102 24-25 0.2826501113322024 1102 26-27 0.3020867160935765 1102 28-29 0.2721702232554293 1102 30-31 0.26709891138845876 1102 32-33 0.25663170882048547 1102 34-35 0.25931986639889004 1102 36-37 0.248278112830846 1102 38-39 0.16613178738884926 1102 40-41 0.19029304589710705 1102 42-43 0.2052514828093024 1102 44-45 0.19776515112047832 1102 46-47 0.2121642737623759 1102 48-49 0.1534773655346484 1102 50-51 0.2320508252836362 1102 52-53 0.23248064162635984 1102 54-55 0.23865136691137678 1102 56-57 0.28617772157313937 1102 58-59 0.2161112955238096 1102 60-61 0.22270143352992022 1102 62-63 0.2246625890011984 1102 64-65 0.24727410228985747 1102 66-67 0.2605754005277632 1102 68-69 0.2968303795534908 1102 70-71 0.3254094426568699 1102 72-73 0.27821103345165454 1102 74-75 0.22444856133727598 1102 76-77 0.2216209435019536 1102 78-79 0.196197370943203 1102 80-81 0.15906197815717604 1102 82-83 0.16764887528024985 1102 84-85 0.16164344864790792 1102 86-87 0.1802644895785619 1102 88-89 0.1496371163599619 1102 90-91 0.11749529292046645 1102 92-93 0.11299939224453226 1102 94-95 0.14004938409993883 1102 96-97 0.15932577810698945 1102 98-99 0.14288019780628503 1102 100-101 0.007132624267452314 1103 1 0.7981613907777572 1103 2 0.3729519646395474 1103 3 0.28652145962814757 1103 4 0.14656759945547293 1103 5 0.13775862396245486 1103 6 0.14320217396853963 1103 7 0.13630970616903682 1103 8 0.1294099950658989 1103 9 0.18260129407216397 1103 10-11 0.15138253020251824 1103 12-13 0.15449954635420937 1103 14-15 0.21884046701588744 1103 16-17 0.24676082807466315 1103 18-19 0.2100929681342052 1103 20-21 0.16535546014498692 1103 22-23 0.21297988557881098 1103 24-25 0.16606024221299265 1103 26-27 0.2183216205531764 1103 28-29 0.2091114601613029 1103 30-31 0.22933906471992316 1103 32-33 0.23296624087292628 1103 34-35 0.23705514320590027 1103 36-37 0.2544496847779669 1103 38-39 0.22839973826486926 1103 40-41 0.19698312871589962 1103 42-43 0.20320290169226496 1103 44-45 0.17548751664121198 1103 46-47 0.12192997206317813 1103 48-49 -0.016972432091925782 1103 50-51 0.0948266335787693 1103 52-53 0.17967228814377734 1103 54-55 0.18547795863058525 1103 56-57 0.23159113551425037 1103 58-59 0.19020242909373763 1103 60-61 0.19834286532820755 1103 62-63 0.14658657542872788 1103 64-65 0.17219775788728242 1103 66-67 0.19840595008658113 1103 68-69 0.29268180949865297 1103 70-71 0.24276192058888313 1103 72-73 0.2567153880264499 1103 74-75 0.2542945942575656 1103 76-77 0.1829061640061056 1103 78-79 0.09888133805160493 1103 80-81 0.2129637793482928 1103 82-83 0.24110526495962858 1103 84-85 0.2517887266216974 1103 86-87 0.2245435039939352 1103 88-89 0.26075535342490497 1103 90-91 0.17046295607485007 1103 92-93 0.15374977410839819 1103 94-95 0.23697891566440177 1103 96-97 0.2117547463779701 1103 98-99 0.1819487591951443 1103 100-101 0.09344601137356179 1104 1 0.9182688882958416 1104 2 0.5984999112644616 1104 3 0.4426019418066076 1104 4 0.22532442555242937 1104 5 0.17532676972362538 1104 6 0.13895933157003526 1104 7 0.0819601850004048 1104 8 0.08704178210462032 1104 9 0.11896712734759518 1104 10-11 0.08745477111460787 1104 12-13 0.10421603352533992 1104 14-15 0.10631754377558167 1104 16-17 0.16168048720249573 1104 18-19 0.07648231284917983 1104 20-21 0.10521049209765465 1104 22-23 0.13274332519711862 1104 24-25 0.15369511681487324 1104 26-27 0.10894092371813002 1104 28-29 0.10071719629601006 1104 30-31 0.12869191646699107 1104 32-33 0.13521927011367296 1104 34-35 0.16891794528777382 1104 36-37 0.11999182333867964 1104 38-39 0.15475840229702698 1104 40-41 0.16299611567109196 1104 42-43 0.15573092177668713 1104 44-45 0.1578196730762471 1104 46-47 0.15904395871876176 1104 48-49 0.19790261019043243 1104 50-51 0.13110516648689696 1104 52-53 0.16062902838030624 1104 54-55 0.1671541732565558 1104 56-57 0.17608350246473492 1104 58-59 0.18936161672106522 1104 60-61 0.16683573106129757 1104 62-63 0.17689969634416514 1104 64-65 0.13841118230116933 1104 66-67 0.10969541260695337 1104 68-69 0.15498470984614698 1104 70-71 0.11803469318297743 1104 72-73 0.1321960126878281 1104 74-75 0.13294056897557738 1104 76-77 0.09938509365449022 1104 78-79 0.15718067039499317 1104 80-81 0.1614555269061171 1104 82-83 0.24818894677555647 1104 84-85 0.21974108939849657 1104 86-87 0.2531623140530286 1104 88-89 0.26201283401468345 1104 90-91 0.22770108058816874 1104 92-93 0.23357783919381347 1104 94-95 0.308399160233364 1104 96-97 0.30311039216691427 1104 98-99 0.2495974498448561 1104 100-101 0.2089286278684277 1105 1 0.9999821424754742 1105 2 0.6180068514796986 1105 3 0.39028372054058025 1105 4 0.223919557628264 1105 5 0.08199585159621137 1105 6 0.033617119146590824 1105 7 0.01624772037990141 1105 8 -0.014729871918824244 1105 9 -0.038651745317537234 1105 10-11 -0.04040809108877852 1105 12-13 -0.04901139956511713 1105 14-15 -0.04166527159785005 1105 16-17 -0.1014319228800602 1105 18-19 -0.07428076530788985 1105 20-21 -0.05876260077861417 1105 22-23 -0.01497513407075246 1105 24-25 -0.06555042701404545 1105 26-27 -0.06885030827341865 1105 28-29 -0.05517083798375921 1105 30-31 -0.08727221231519877 1105 32-33 -0.06633397198105939 1105 34-35 -0.11273985674758791 1105 36-37 -0.058181889239264706 1105 38-39 -0.07578326171908856 1105 40-41 -0.10280777337618474 1105 42-43 -0.12249623952602917 1105 44-45 -0.06310359390273845 1105 46-47 -0.046414263258050426 1105 48-49 -0.14186187522815175 1105 50-51 -0.10920305016888676 1105 52-53 -0.10243614080572883 1105 54-55 -0.08114417541209207 1105 56-57 -0.1251551039842269 1105 58-59 -0.11040475568533026 1105 60-61 -0.10748987576395308 1105 62-63 -0.0666745978415193 1105 64-65 -0.06296744794424569 1105 66-67 -0.09434751369274608 1105 68-69 -0.023377979765044188 1105 70-71 -0.07955868290725476 1105 72-73 -0.13497716569249008 1105 74-75 -0.13529960147909748 1105 76-77 -0.1887916371274727 1105 78-79 -0.10164430197580998 1105 80-81 -0.045457056807372 1105 82-83 -0.04508806407902455 1105 84-85 -0.04772638466498336 1105 86-87 0.0025370615994688706 1105 88-89 0.03649665646742051 1105 90-91 0.0135209757655943 1105 92-93 -0.017474821314486633 1105 94-95 -0.038448156835301006 1105 96-97 -0.06026123968982944 1105 98-99 -0.1051550328587254 1105 100-101 -0.1909838317609065 1106 1 0.8793046090633467 1106 2 0.48553678006580725 1106 3 0.30685964665074295 1106 4 0.2075105883043591 1106 5 0.10041988316864803 1106 6 0.014512876514423567 1106 7 -0.07030103581671909 1106 8 -0.09900624463907803 1106 9 -0.15732340907790388 1106 10-11 -0.1796998708085411 1106 12-13 -0.17695990353860935 1106 14-15 -0.19030242141048603 1106 16-17 -0.17811928692380974 1106 18-19 -0.17437237711193632 1106 20-21 -0.1741215378719616 1106 22-23 -0.30295905388151567 1106 24-25 -0.26873284790685403 1106 26-27 -0.22107306673839133 1106 28-29 -0.22935557777701376 1106 30-31 -0.24335307988217636 1106 32-33 -0.23732510822333808 1106 34-35 -0.16298478941438788 1106 36-37 -0.1718092861784939 1106 38-39 -0.3233241679169794 1106 40-41 -0.31442322504342 1106 42-43 -0.2884041985649617 1106 44-45 -0.26859744536361774 1106 46-47 -0.41126834290034253 1106 48-49 -0.31597113082782613 1106 50-51 -0.31386893887690803 1106 52-53 -0.25623225581418296 1106 54-55 -0.2091018112225953 1106 56-57 -0.20116019560346388 1106 58-59 -0.25438924615905734 1106 60-61 -0.22725660404197612 1106 62-63 -0.28994608263074895 1106 64-65 -0.2623771477722201 1106 66-67 -0.22932252394720365 1106 68-69 -0.21615671588335061 1106 70-71 -0.1861437787041922 1106 72-73 -0.2048446326078306 1106 74-75 -0.17975168721997647 1106 76-77 -0.27685473693458107 1106 78-79 -0.1879640259817421 1106 80-81 -0.16190263693463436 1106 82-83 -0.1271193755647957 1106 84-85 -0.09657154253468292 1106 86-87 -0.15619793507826785 1106 88-89 -0.10956892336791668 1106 90-91 -0.12571141663374163 1106 92-93 -0.16768867392593734 1106 94-95 -0.2086555822061058 1106 96-97 -0.2541365530655213 1106 98-99 -0.3597209871029534 1106 100-101 -0.534680139329236 1107 1 -5.352333957010931 1107 2 -2.302291728901224 1107 3 -1.1274585183091084 1107 4 -0.6049169870073285 1107 5 -0.404819461209442 1107 6 -0.3253225760827192 1107 7 -0.3134617425918762 1107 8 -0.3083594058703909 1107 9 -0.39417072473487735 1107 10-11 -0.3643334390188855 1107 12-13 -0.3712913326521914 1107 14-15 -0.49158456020399655 1107 16-17 -0.5317602460543398 1107 18-19 -0.45928747319369734 1107 20-21 -0.41113700068312653 1107 22-23 -0.45822754453630665 1107 24-25 -0.5201420855119707 1107 26-27 -0.5686292029075375 1107 28-29 -0.5213884224302916 1107 30-31 -0.5474105789966472 1107 32-33 -0.5572715894418039 1107 34-35 -0.5455932449852625 1107 36-37 -0.5502742599678214 1107 38-39 -0.5394827002140659 1107 40-41 -0.5769023058177964 1107 42-43 -0.6856507481590342 1107 44-45 -0.5929413449161274 1107 46-47 -0.5457689109543153 1107 48-49 -0.5358973451650897 1107 50-51 -0.5716581863317884 1107 52-53 -0.5733710083471237 1107 54-55 -0.6315395828163233 1107 56-57 -0.5891060237828754 1107 58-59 -0.7195539328550282 1107 60-61 -0.7364596732112219 1107 62-63 -0.660684422313679 1107 64-65 -0.6943634651874504 1107 66-67 -0.6340117259442053 1107 68-69 -0.6869726275520165 1107 70-71 -0.8194322956463296 1107 72-73 -0.8085668202927963 1107 74-75 -0.7007170551413324 1107 76-77 -0.8248603593474613 1107 78-79 -0.7157966151300386 1107 80-81 -0.7399218932212577 1107 82-83 -0.6914456163009461 1107 84-85 -0.7329588371380851 1107 86-87 -0.8162145986352414 1107 88-89 -0.662891773485164 1107 90-91 -0.663275870688615 1107 92-93 -0.6359409970780447 1107 94-95 -0.6719411754719999 1107 96-97 -0.6876909382893928 1107 98-99 -0.7923806484286651 1107 100-101 -1.015459596635715 1108 1 -1.835759223157762 1108 2 -1.0589216458111785 1108 3 -1.0573617240521358 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2305 3 0.02848678238343183 2305 4 0.048601048562375126 2305 5 0.01931541111110846 2305 6 0.013999692310399325 2305 7 -0.005303063416477016 2305 8 0.004334024622153265 2305 9 0.0061233372911999595 2305 10-11 -0.02470335237079979 2305 12-13 -0.04466532363304054 2305 14-15 -0.04054307907100707 2305 16-17 -0.048612755697320154 2305 18-19 -0.0794113506474119 2305 20-21 -0.03930673649408334 2305 22-23 -0.013320511758216469 2305 24-25 -0.047648383577374886 2305 26-27 -0.04923728811289152 2305 28-29 -0.06809092705516662 2305 30-31 -0.0033297638052260936 2305 32-33 -0.005287568635765183 2305 34-35 -0.027066162096510027 2305 36-37 -0.010936200641666005 2305 38-39 -0.03328079896031966 2305 40-41 3.508671007068642E-4 2305 42-43 0.04138824495440474 2305 44-45 0.07953353092658944 2305 46-47 0.08837037454868124 2305 48-49 0.0963661390433046 2305 50-51 0.041220065860741784 2305 52-53 -0.020911032418744924 2305 54-55 -0.05613255258160876 2305 56-57 -0.06863211101006783 2305 58-59 -0.013767429713787749 2305 60-61 -0.024553563254933408 2305 62-63 -0.05241597954714905 2305 64-65 -0.08168771711980582 2305 66-67 -0.015848004689551942 2305 68-69 -0.03147320878479576 2305 70-71 -0.05269605919810516 2305 72-73 0.02055826405829464 2305 74-75 -0.014716840308118151 2305 76-77 0.029262654173223268 2305 78-79 0.04591450962387711 2305 80-81 0.025733702580033935 2305 82-83 0.040385811462606114 2305 84-85 0.0306578666339945 2305 86-87 0.039885265797011016 2305 88-89 0.02774420026951674 2305 90-91 0.027930433336770477 2305 92-93 1.5482045727566174E-4 2305 94-95 -0.030250125573161313 2305 96-97 0.018114690756945606 2305 98-99 0.04520078356223678 2305 100-101 0.15039967585844138 2306 1 -4.249550057800107 2306 2 -1.5164468872122363 2306 3 -0.6514211986955871 2306 4 -0.38927331670358 2306 5 -0.32803110449817297 2306 6 -0.2863347120120565 2306 7 -0.24093555107445752 2306 8 -0.2082803159069968 2306 9 -0.21779280476383178 2306 10-11 -0.206599205632223 2306 12-13 -0.17781811002189585 2306 14-15 -0.2984816890555635 2306 16-17 -0.28588631418225674 2306 18-19 -0.25919132797204725 2306 20-21 -0.2338038958452202 2306 22-23 -0.28246090270237545 2306 24-25 -0.2839762607741818 2306 26-27 -0.25429405709875397 2306 28-29 -0.22070608346457732 2306 30-31 -0.21022694464844704 2306 32-33 -0.253697150723049 2306 34-35 -0.26703474819880313 2306 36-37 -0.25340905219478316 2306 38-39 -0.2315247692677076 2306 40-41 -0.22023160787080087 2306 42-43 -0.24341501975365532 2306 44-45 -0.3167188618815544 2306 46-47 -0.2582237506662395 2306 48-49 -0.1638952845394286 2306 50-51 -0.2834554383523127 2306 52-53 -0.20900601868618196 2306 54-55 -0.29703074897005166 2306 56-57 -0.2930274155272343 2306 58-59 -0.24359515087142114 2306 60-61 -0.2471821233815561 2306 62-63 -0.20808064515463087 2306 64-65 -0.2637123160349262 2306 66-67 -0.21778496168064976 2306 68-69 -0.34555346084832195 2306 70-71 -0.31033243403229704 2306 72-73 -0.2644444566223143 2306 74-75 -0.23478185618402847 2306 76-77 -0.20081292405003381 2306 78-79 -0.20193854348719498 2306 80-81 -0.18571676511852075 2306 82-83 -0.22326936259857177 2306 84-85 -0.2102482921104354 2306 86-87 -0.28830145976851895 2306 88-89 -0.25862804564089004 2306 90-91 -0.26569422316647007 2306 92-93 -0.3096840245923289 2306 94-95 -0.3300344063317766 2306 96-97 -0.28515577486469823 2306 98-99 -0.28766357536288467 2306 100-101 -0.1486990794873897 2307 1 -5.289034065468286 2307 2 -2.3638186796114873 2307 3 -1.2411214824570322 2307 4 -0.6506254174060899 2307 5 -0.5028976264715652 2307 6 -0.3649113917450961 2307 7 -0.38218431407882036 2307 8 -0.3080381954803215 2307 9 -0.41326787753558136 2307 10-11 -0.37148463934730813 2307 12-13 -0.3402018859180842 2307 14-15 -0.5516066580565919 2307 16-17 -0.5212644224148519 2307 18-19 -0.4920851513094675 2307 20-21 -0.44570063534446547 2307 22-23 -0.4948194644369366 2307 24-25 -0.4710501472613089 2307 26-27 -0.4848519181092925 2307 28-29 -0.49302611632293747 2307 30-31 -0.47680990322531613 2307 32-33 -0.554563919894413 2307 34-35 -0.507230341589441 2307 36-37 -0.4635942232351482 2307 38-39 -0.45099522332840536 2307 40-41 -0.4468219542534442 2307 42-43 -0.5314238678840155 2307 44-45 -0.43037661402571814 2307 46-47 -0.434156131008109 2307 48-49 -0.37795059732584235 2307 50-51 -0.3998729447040006 2307 52-53 -0.5268357745850025 2307 54-55 -0.518949238198033 2307 56-57 -0.5246327670454605 2307 58-59 -0.5589567433651084 2307 60-61 -0.5384391088588316 2307 62-63 -0.47316670137685435 2307 64-65 -0.5800234051625637 2307 66-67 -0.6042375989487851 2307 68-69 -0.585458775619486 2307 70-71 -0.5254445103556407 2307 72-73 -0.6023074750619344 2307 74-75 -0.46235683924296467 2307 76-77 -0.43723203207485994 2307 78-79 -0.42771185486073904 2307 80-81 -0.40069904333231676 2307 82-83 -0.4515824134450668 2307 84-85 -0.4897366006605921 2307 86-87 -0.45775911923585255 2307 88-89 -0.4962583038919348 2307 90-91 -0.43369012437144505 2307 92-93 -0.42927227109892385 2307 94-95 -0.3769979119384672 2307 96-97 -0.40587441969921656 2307 98-99 -0.44286869150249686 2307 100-101 -0.3926197347135627 2308 1 0.447681415691072 2308 2 0.19862423089149672 2308 3 -0.055502319267027644 2308 4 -0.0015218002832213529 2308 5 -0.24801105521459732 2308 6 -0.3338346315876777 2308 7 -0.3134865955684063 2308 8 -0.3818476557374737 2308 9 -0.5385319322797386 2308 10-11 -0.4797581303530265 2308 12-13 -0.5372772905728098 2308 14-15 -0.7158257289396914 2308 16-17 -0.6886346547319633 2308 18-19 -0.6788009568911875 2308 20-21 -0.6690323115983006 2308 22-23 -0.7077460051507956 2308 24-25 -0.6848788832577668 2308 26-27 -0.7913068240029091 2308 28-29 -0.7361009961516416 2308 30-31 -0.7161292037178555 2308 32-33 -0.7571330753779364 2308 34-35 -0.8072213568383333 2308 36-37 -0.772949263804847 2308 38-39 -0.6692077641138354 2308 40-41 -0.6458303093143343 2308 42-43 -0.5785874299452232 2308 44-45 -0.5497433345775775 2308 46-47 -0.519555134965394 2308 48-49 -0.5360336341429601 2308 50-51 -0.7569126939810644 2308 52-53 -0.7837153105001775 2308 54-55 -0.6816569663233878 2308 56-57 -0.705021097118447 2308 58-59 -0.7444131724120169 2308 60-61 -0.7344438350127476 2308 62-63 -0.7331745772639664 2308 64-65 -0.6704429741952325 2308 66-67 -0.6790007245822665 2308 68-69 -0.7137211290306027 2308 70-71 -0.5805349400785076 2308 72-73 -0.45655309418472 2308 74-75 -0.49456037732721114 2308 76-77 -0.4391774480703887 2308 78-79 -0.36343436969121257 2308 80-81 -0.3409633803433607 2308 82-83 -0.393707333694465 2308 84-85 -0.39604885396119016 2308 86-87 -0.36854041065677023 2308 88-89 -0.35268285861641147 2308 90-91 -0.40141146250063997 2308 92-93 -0.38976457096325134 2308 94-95 -0.3239344393440149 2308 96-97 -0.34390739872835496 2308 98-99 -0.35538808836596303 2308 100-101 -0.2616439021593244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 6.0 11 80.0 12 256.0 13 728.0 14 2002.0 15 4062.0 16 6652.0 17 9166.0 18 12171.0 19 15295.0 20 18981.0 21 21953.0 22 24843.0 23 29470.0 24 35990.0 25 45144.0 26 58351.0 27 77260.0 28 101471.0 29 134130.0 30 176534.0 31 232112.0 32 312307.0 33 438456.0 34 675628.0 35 1155476.0 36 2331599.0 37 4401267.0 38 3788096.0 39 501112.0 40 190.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.54831968251432 13.534296061254572 13.660074036089531 40.25731022014158 2 24.329892405529392 17.86104897285485 29.138140940789782 28.670917680825976 3 29.659919779822964 17.287691806903226 20.295407749397228 32.75698066387658 4 30.676627434468283 21.70454461456836 19.01978866574479 28.599039285218563 5 31.479130352175392 24.350021367772907 21.602264025732218 22.56858425431948 6 28.4559395427543 28.749866194759655 20.00161113829042 22.792583124195627 7 23.595250304090378 22.008833472910563 32.842561263636156 21.553354959362903 8 23.38262659070818 23.587721620490285 26.977169198540146 26.052482590261388 9 23.099712349532414 22.04878340579577 29.284471172944265 25.567033071727547 10-11 25.732482738097357 27.861977738640793 22.68223999964957 23.72329952361228 12-13 25.14225447662371 23.771332524980856 25.566533440906813 25.51987955748862 14-15 25.219094959149363 24.458342014133667 25.358461158973768 24.964101867743203 16-17 25.783626454644338 24.36460990331254 24.713317994895277 25.138445647147844 18-19 25.565106413151707 24.598796450951173 24.610787590648773 25.225309545248347 20-21 25.551623225249724 24.63575202104089 24.707370334851205 25.105254418858173 22-23 25.573979993413086 24.59620932149587 24.720186207615907 25.109624477475133 24-25 25.498597337802725 24.579399824294214 24.74934959017953 25.17265324772353 26-27 25.471316126139126 24.662424093758666 24.68839120792116 25.17786857218105 28-29 25.605374604025467 24.49763147613941 24.5185749050633 25.378419014771826 30-31 25.43716339930835 24.509016764603462 24.57024893393811 25.48357090215008 32-33 25.38582373862266 24.63863935574438 24.67900337589676 25.296533529736205 34-35 25.549545269112688 24.547302713280043 24.533460198526644 25.369691819080625 36-37 25.478417043625573 24.500215183465805 24.55732709283031 25.46404068007831 38-39 25.461309821208822 24.644498982532635 24.535973008437328 25.35821818782122 40-41 25.5122276772478 24.57209357907322 24.457315375460926 25.458363368218055 42-43 25.394578307480746 24.52307500457881 24.610000500999675 25.472346186940776 44-45 25.42346791973164 24.54337507326778 24.695310752575423 25.337846254425155 46-47 25.53911428639384 24.46224604387724 24.497108143176558 25.501531526552363 48-49 25.499994524545777 24.43612061330563 24.532758958185326 25.53112590396327 50-51 25.41850241068449 24.618408671729412 24.585730762776105 25.377358154809993 52-53 25.51000329345686 24.571963538174668 24.537868867852985 25.380164300515485 54-55 25.342931537984125 24.507665158100984 24.671937612126055 25.477465691788836 56-57 25.41974040890954 24.551665990054676 24.64163719044146 25.386956410594326 58-59 25.58828879004639 24.43718040523141 24.5039872080817 25.470543596640493 60-61 25.39756837104104 24.511873029458084 24.53513126770335 25.555427331797524 62-63 25.283160410879695 24.67810037356589 24.6469502043909 25.391789011163514 64-65 25.473984548111435 24.57541630886365 24.541844738296263 25.408754404728658 66-67 25.425903201699185 24.493563756003876 24.59533797424681 25.485195068050132 68-69 25.39554399756235 24.593885028873235 24.60139319203294 25.40917778153148 70-71 25.47859545291714 24.58950839976034 24.488931934748972 25.442964212573543 72-73 25.413653254020247 24.56178270467 24.551738756321697 25.472825284988048 74-75 25.341983608276298 24.63172759744375 24.658824014146262 25.36746478013369 76-77 25.50532269393419 24.532962374546887 24.54038497240481 25.421329959114114 78-79 25.44148542843822 24.50027678178617 24.58620643869448 25.47203135108113 80-81 25.437652644059995 24.615075518171913 24.56353141254257 25.383740425225525 82-83 25.47027579895075 24.60877195672129 24.49121498443479 25.42973725989317 84-85 25.349418207246465 24.59866467151975 24.574381243118527 25.47753587811526 86-87 25.369181003049324 24.667312582135377 24.641037474572723 25.322468940242576 88-89 25.513361415559956 24.540822879211095 24.514592253043787 25.431223452185165 90-91 25.397179893482598 24.603581546433478 24.51635304296886 25.48288551711507 92-93 25.336354121890146 24.70139033545461 24.569703090966318 25.39255245168893 94-95 25.40596459978629 24.69426784832782 24.496160798998684 25.40360675288721 96-97 25.310648040018375 24.656299326781667 24.565955120488887 25.467097512711074 98-99 25.39247685855755 24.729018516199687 24.509014441771313 25.36949018347145 100-101 25.56052760467129 24.65133639609308 24.385881856611704 25.40225414262393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 67.0 1 40.5 2 11.5 3 11.5 4 10.0 5 11.5 6 21.0 7 29.5 8 38.5 9 46.5 10 59.5 11 73.5 12 107.0 13 167.5 14 224.5 15 307.0 16 457.5 17 629.0 18 946.0 19 1530.5 20 2358.0 21 3592.5 22 5480.0 23 8162.0 24 11376.0 25 14928.0 26 18922.0 27 24157.0 28 30857.5 29 39676.5 30 50586.0 31 62278.0 32 74901.5 33 89938.5 34 107971.5 35 128057.5 36 152689.5 37 180340.5 38 209978.0 39 242814.5 40 275996.5 41 308146.5 42 341683.5 43 377796.0 44 413832.0 45 453197.0 46 495051.0 47 532728.5 48 569533.5 49 599796.5 50 614952.0 51 626775.0 52 642008.5 53 650976.0 54 650157.5 55 638542.0 56 614356.0 57 580777.5 58 540220.0 59 494133.0 60 440969.5 61 382312.0 62 323775.5 63 265815.0 64 211929.0 65 168175.5 66 135042.5 67 111491.5 68 92761.0 69 78296.5 70 67607.5 71 57817.5 72 50417.0 73 45085.0 74 41674.5 75 38624.0 76 35427.5 77 32175.0 78 27462.5 79 23110.5 80 20256.0 81 18182.5 82 15942.5 83 13170.5 84 10071.5 85 7328.0 86 5088.0 87 3043.0 88 1506.0 89 715.5 90 425.0 91 275.5 92 152.5 93 79.0 94 47.5 95 26.5 96 11.5 97 8.0 98 7.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01715855435038822 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.7377031273056592E-5 32-33 2.3954902363924516E-5 34-35 2.3954902363924516E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.002501576232575546 48-49 8.760650007378109E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.0266386727396222E-5 58-59 3.422128909132074E-6 60-61 0.006235118872438639 62-63 0.0062659180326208284 64-65 0.0014475605285628674 66-67 1.0266386727396223E-4 68-69 1.6426218763833955E-4 70-71 9.581960945569807E-5 72-73 0.0 74-75 0.0 76-77 3.422128909132074E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 6.844257818264148E-6 86-87 6.844257818264148E-6 88-89 3.079916018218867E-5 90-91 0.002131986310389282 92-93 2.2928263691184898E-4 94-95 3.422128909132074E-6 96-97 3.422128909132074E-6 98-99 0.0010232165438304902 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4610788E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.82704965787302 #Duplication Level Percentage of deduplicated Percentage of total 1 80.35994076897094 52.09497870733814 2 11.925531301040552 15.461940196981496 3 3.3578309998044062 6.530348309011968 4 1.445961751823778 3.7494973715546047 5 0.7640638843894457 2.4766003687550975 6 0.4674129351258464 1.818060093368126 7 0.29992872817709776 1.361044618875159 8 0.2170715618625046 1.1257687136138113 9 0.1618425504430014 0.9442597548892743 >10 0.936969973085183 11.027274317216694 >50 0.05139297423921652 2.2470217752045807 >100 0.012031060369141195 1.1546053088128754 >500 2.151066887999927E-5 0.008600464378200518 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017863512905669427 0.0 0.0 0.0 2.0532773454792444E-5 2 0.0017863512905669427 4.106554690958489E-5 0.0 6.844257818264148E-6 2.7377031273056592E-5 3 0.0018342610952947918 4.790980472784904E-5 0.0 6.844257818264148E-6 4.790980472784904E-5 4 0.00184794961093132 4.790980472784904E-5 0.0 6.844257818264148E-6 8.213109381916978E-5 5 0.00184794961093132 4.790980472784904E-5 0.0 6.844257818264148E-6 1.0266386727396223E-4 6 0.0018547938687495842 4.790980472784904E-5 0.0 6.844257818264148E-6 1.2319664072875467E-4 7 0.0018753266422043766 4.790980472784904E-5 0.0 6.844257818264148E-6 1.3004089854701882E-4 8 0.001882170900022641 4.790980472784904E-5 0.0 6.844257818264148E-6 1.5057367200181128E-4 9 0.001882170900022641 4.790980472784904E-5 0.0 6.844257818264148E-6 1.9163921891139616E-4 10-11 0.001899281544568301 4.790980472784904E-5 0.0 1.0266386727396222E-5 2.2243837909358483E-4 12-13 0.0019266585758413576 4.790980472784904E-5 0.0 2.0532773454792444E-5 2.5665966818490553E-4 14-15 0.001964301993841811 6.159832036437734E-5 0.0 2.7377031273056592E-5 2.8403669945796213E-4 16-17 0.00201221179856966 6.844257818264149E-5 0.0 5.133193363698111E-5 3.045694729127546E-4 18-19 0.002272293595663697 7.87089649100377E-5 0.0 6.159832036437734E-5 3.559014065497357E-4 20-21 0.00395940314786581 8.213109381916978E-5 0.0 6.159832036437734E-5 3.867005667319244E-4 22-23 0.005061328656606337 8.555322272830185E-5 0.0 7.87089649100377E-5 4.003890823684527E-4 24-25 0.0063240942240760725 8.897535163743393E-5 0.0 9.2397480546566E-5 4.414546292780376E-4 26-27 0.008202842995189582 8.897535163743393E-5 0.0 1.0950812509222637E-4 5.03052949642415E-4 28-29 0.010095280281939619 8.897535163743393E-5 0.0 1.1635238291049052E-4 5.54384883279396E-4 30-31 0.011915852861597883 1.0266386727396223E-4 0.0 1.1635238291049052E-4 5.95450430188981E-4 32-33 0.013975974464895392 1.0266386727396223E-4 0.0 1.2661876963788673E-4 6.330938481894338E-4 34-35 0.016227735287104295 1.0950812509222637E-4 0.0 1.3004089854701882E-4 6.570487505533582E-4 36-37 0.018085951284763015 1.1635238291049052E-4 0.0 1.437294141835471E-4 6.707372661898866E-4 38-39 0.01968750761423682 1.2319664072875467E-4 0.0 1.574179298200754E-4 7.289134576451318E-4 40-41 0.021935846307536595 1.2319664072875467E-4 0.0 1.711064454566037E-4 7.562904889181884E-4 42-43 0.023958324492833652 1.2661876963788673E-4 0.0 1.84794961093132E-4 7.939339069186412E-4 44-45 0.026473589241045724 1.3688515636528297E-4 0.0 1.84794961093132E-4 8.452658405556223E-4 46-47 0.028437891234887536 1.3688515636528297E-4 0.0 1.9163921891139616E-4 8.692207429195468E-4 48-49 0.030340594908364972 1.3688515636528297E-4 0.0 1.9506134782052825E-4 8.931756452834714E-4 50-51 0.03234254032020723 1.3688515636528297E-4 0.0 2.0532773454792446E-4 9.68462481284377E-4 52-53 0.03617532469843516 1.3688515636528297E-4 0.0 2.0532773454792446E-4 0.0010300608016487545 54-55 0.04077124382339953 1.4030728527441504E-4 0.0 2.0532773454792446E-4 0.0010403271883761506 56-57 0.04466904865090096 1.5057367200181128E-4 0.0 2.121719923661886E-4 0.001060859961830943 58-59 0.04840601341967319 1.5057367200181128E-4 0.0 2.4639328145750934E-4 0.0010848148641948675 60-61 0.052502301723904285 1.5057367200181128E-4 0.0 2.5665966818490553E-4 0.0011224582821953203 62-63 0.05708111020432299 1.5057367200181128E-4 0.0 2.7377031273056595E-4 0.0011361467978318485 64-65 0.062289590404022016 1.5057367200181128E-4 0.0 2.7377031273056595E-4 0.0011532574423775092 66-67 0.06768970982263242 1.5399580091094334E-4 0.0 2.8061457054883007E-4 0.0011566795712866411 68-69 0.07350732896815695 1.574179298200754E-4 0.0 2.8061457054883007E-4 0.0012045893760144902 70-71 0.09077881357254652 1.7795070327486785E-4 0.0 2.8061457054883007E-4 0.001293564727651924 72-73 0.10726663065674485 1.7795070327486785E-4 0.0 2.8061457054883007E-4 0.0013312081456523768 74-75 0.11492193302647331 1.8137283218399994E-4 0.0 2.8061457054883007E-4 0.0013620073058345655 76-77 0.12393924270203632 1.84794961093132E-4 0.0 3.0114734400362255E-4 0.001379117950380226 78-79 0.1338155067337915 1.84794961093132E-4 0.0 3.0114734400362255E-4 0.0014167613683806787 80-81 0.1454781220561136 1.9506134782052825E-4 0.0 3.0114734400362255E-4 0.0014372941418354713 82-83 0.15908792872773186 2.0190560563879237E-4 0.0 3.21680117458415E-4 0.0014578269152902637 84-85 0.17623279456248356 2.0532773454792446E-4 0.0 3.21680117458415E-4 0.0014988924621998486 86-87 0.19564995399289895 2.0532773454792446E-4 0.0 3.285243752766791E-4 0.001563912911473358 88-89 0.2207820687015649 2.0532773454792446E-4 0.0 3.285243752766791E-4 0.0015741792982007542 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTGT 3625 0.0 8.256472 1 CCGTCTA 1535 3.6379788E-12 8.045437 4 GTCGGAT 2710 0.0 7.1874776 1 CCGCGTA 800 4.8641535E-4 7.124853 2 TCGGATT 3020 0.0 6.7631054 2 CTCGTTT 3880 0.0 6.489423 1 CCCCCGT 3590 0.0 6.4843073 1 CGGGTTT 3240 0.0 6.451632 1 GTCGGTA 2065 8.913048E-11 6.4416914 1 CCGGATA 2265 2.1827873E-11 6.291261 2 CCCGGAT 6260 0.0 6.1471334 1 CCCGGGA 9905 0.0 6.091316 1 CCCGTAT 1880 2.0461812E-8 6.064784 1 GCCCCGT 4465 0.0 6.0647836 1 GTCGTTT 2850 0.0 6.0009437 1 TCCCGTT 4365 0.0 5.9849854 2 CCGGGAT 4935 0.0 5.9685173 1 CGGTTTT 3360 0.0 5.9373775 3 GCGGGTT 2805 0.0 5.927849 1 CCCGTCT 5055 0.0 5.9208126 1 >>END_MODULE