FastQCFastQC Report
Thu 26 May 2016
SRR1051301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4495062
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT118752826.418501012889255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4038828.985015112138608No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1726133.8400582683842845No Hit
ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT269030.5985011997609821No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA249410.5548533034694516No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230180.5120730259115447No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA210580.4684696228884051No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG137380.3056242605774959No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGG130180.28960668395675077No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA111750.24860613713448224No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT108910.24228809302296608No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC104690.2329000133924738No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC98850.21990797902231382No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC89630.19939658229408183No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.1934789775980843No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA82470.18346799221011856No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA79080.17592638321785106No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCA76120.16934137949598915No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCC72910.16220020991924028No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGA71480.1590189412292867No Hit
ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT62940.1400203156263473No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG53300.11857456026190516No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTGGG45540.10131117212621317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC1365950.093.645441
TATCTCG1365000.093.578382
ATCTCGA1379200.092.5666963
TCTCGAG1380500.092.390074
ATATGGA461000.092.174971
CTCGAGG1385150.092.097055
TCGAGGG1388050.091.880696
AGGGCGC1398850.091.388639
TATGGAT463500.091.267892
CGAGGGC1398550.091.238427
GAGGGCG1405850.090.845748
ATGGATC481900.087.615523
GGATCCG486050.085.9976965
TGGATCC492050.085.200034
GATCCGG496600.084.1707156
ATCCGGC505600.082.616067
TCCGGCG513000.081.526178
CCGGCGC518300.080.967449
CGCCGGA1429750.045.8412914-15
CCGGATC1424050.045.81797816-17
Produced by FastQC (version 0.11.5)