##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051301_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4495062 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65653710671844 34.0 31.0 34.0 31.0 34.0 2 32.74188431661232 34.0 31.0 34.0 31.0 34.0 3 32.808175504587034 34.0 31.0 34.0 31.0 34.0 4 36.137465957088025 37.0 37.0 37.0 35.0 37.0 5 36.127032063184004 37.0 37.0 37.0 35.0 37.0 6 36.041363389425996 37.0 37.0 37.0 35.0 37.0 7 36.080129039376985 37.0 37.0 37.0 35.0 37.0 8 36.03891270020302 37.0 37.0 37.0 35.0 37.0 9 37.673804499248284 39.0 39.0 39.0 35.0 39.0 10-11 37.64566651138516 39.0 38.5 39.0 35.0 39.0 12-13 37.6523704233668 39.0 39.0 39.0 35.0 39.0 14-15 39.23203995851448 41.0 40.0 41.0 36.0 41.0 16-17 39.142583127885665 41.0 39.5 41.0 36.0 41.0 18-19 38.81374316972713 41.0 38.0 41.0 35.5 41.0 20-21 38.41053649538093 40.5 38.0 41.0 35.0 41.0 22-23 38.53818990705801 40.0 38.0 41.0 35.0 41.0 24-25 38.67288916148431 41.0 38.0 41.0 35.0 41.0 26-27 38.736032228254025 41.0 38.0 41.0 35.0 41.0 28-29 38.64501868939739 41.0 38.0 41.0 35.0 41.0 30-31 38.184284332451924 40.0 37.0 40.5 35.0 41.0 32-33 36.481575115092966 36.0 35.0 40.0 34.5 41.0 34-35 36.02321002468932 35.0 35.0 39.5 33.5 41.0 36-37 35.89554226393318 35.0 35.0 39.0 33.0 41.0 38-39 35.85093765113807 35.0 35.0 39.0 33.0 41.0 40-41 35.85748583668034 35.0 35.0 39.0 33.0 41.0 42-43 35.8469872050708 35.0 35.0 39.0 33.0 41.0 44-45 35.71303376905591 35.0 35.0 39.0 33.0 41.0 46-47 35.31131928769837 35.0 35.0 39.0 32.0 41.0 48-49 34.15370677868292 35.0 35.0 39.0 25.0 41.0 50-51 33.890032440041985 35.0 35.0 39.0 23.5 41.0 52-53 33.88967849609193 35.0 35.0 39.0 24.0 41.0 54-55 33.55110207601141 35.0 34.0 39.0 24.0 41.0 56-57 33.177357064262964 35.0 34.0 39.0 22.5 41.0 58-59 32.91257628927032 35.0 34.0 38.0 22.0 41.0 60-61 32.68416853872093 35.0 34.0 38.0 20.0 41.0 62-63 32.40718604103792 35.0 33.5 37.0 19.0 40.5 64-65 32.13819709272086 35.0 33.0 36.5 18.5 40.0 66-67 31.854976193876748 35.0 33.0 36.0 16.5 40.0 68-69 31.535712633107174 35.0 33.0 35.5 9.5 39.0 70-71 31.180293842443106 35.0 33.0 35.0 6.0 39.0 72-73 30.852591799623674 35.0 33.0 35.0 2.0 38.5 74-75 30.56949659426277 35.0 33.0 35.0 2.0 37.0 76-77 30.28222502826435 35.0 32.0 35.0 2.0 37.0 78-79 30.034866927308233 35.0 32.0 35.0 2.0 36.0 80-81 29.806622022121164 35.0 32.0 35.0 2.0 36.0 82-83 29.619815366284158 35.0 32.0 35.0 2.0 35.5 84-85 29.439931974241958 35.0 31.5 35.0 2.0 35.0 86-87 29.271973556760727 35.0 31.0 35.0 2.0 35.0 88-89 29.123263038418603 35.0 31.0 35.0 2.0 35.0 90-91 28.948467117917396 35.0 31.0 35.0 2.0 35.0 92-93 28.78024585645315 35.0 31.0 35.0 2.0 35.0 94-95 28.593833522207255 35.0 30.5 35.0 2.0 35.0 96-97 28.39921974379886 35.0 30.0 35.0 2.0 35.0 98-99 28.18412371175303 35.0 30.0 35.0 2.0 35.0 100-101 27.45529349762028 34.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21582759834785747 1101 2 0.2181912731339395 1101 3 0.21052841704661773 1101 4 0.21852057157967408 1101 5 0.1890569309143899 1101 6 0.24683188405263223 1101 7 0.23599172020593073 1101 8 0.25739079131196974 1101 9 0.3173571036976526 1101 10-11 0.35783465619221033 1101 12-13 0.29839469824050724 1101 14-15 0.37450246141595045 1101 16-17 0.36872392450857916 1101 18-19 0.3262577024871831 1101 20-21 0.36273150302593393 1101 22-23 0.367392841917912 1101 24-25 0.32999929357534796 1101 26-27 0.2956242278260106 1101 28-29 0.2998620887981289 1101 30-31 0.25511322768006295 1101 32-33 0.11636011848712258 1101 34-35 0.07512048298999474 1101 36-37 0.049422013063541215 1101 38-39 0.09146699501702926 1101 40-41 0.09883966442490788 1101 42-43 0.11531982584173761 1101 44-45 0.09693836359201669 1101 46-47 0.08944026410858896 1101 48-49 0.01831633437473812 1101 50-51 -0.17262447717403973 1101 52-53 -0.2117980907514081 1101 54-55 -0.39193067219847677 1101 56-57 -0.3899365846256373 1101 58-59 -0.44856880012775946 1101 60-61 -0.5638593303981096 1101 62-63 -0.577556838219472 1101 64-65 -0.513542392015566 1101 66-67 -0.48746375060604663 1101 68-69 -0.5020622367426597 1101 70-71 -0.5487476061135084 1101 72-73 -0.620330881229318 1101 74-75 -0.6687001036708331 1101 76-77 -0.6302419263399308 1101 78-79 -0.6089037512573334 1101 80-81 -0.5919305089434772 1101 82-83 -0.5974609670269437 1101 84-85 -0.5966172389929341 1101 86-87 -0.5443350578783814 1101 88-89 -0.5312012552028591 1101 90-91 -0.5180748994454945 1101 92-93 -0.5364729308817502 1101 94-95 -0.5110260684666414 1101 96-97 -0.4818813231347825 1101 98-99 -0.43998139097307387 1101 100-101 -0.4316585964426558 1102 1 0.16987824334631796 1102 2 0.16703886610473262 1102 3 0.22153434428923902 1102 4 0.14631216929046786 1102 5 0.19904904363180265 1102 6 0.2232565660182857 1102 7 0.18855350902340007 1102 8 0.17657690971205398 1102 9 0.2583829761510472 1102 10-11 0.2582674944454695 1102 12-13 0.25431465776465245 1102 14-15 0.2763718456885158 1102 16-17 0.2702984102446564 1102 18-19 0.2691101912967895 1102 20-21 0.29865457369693615 1102 22-23 0.3105104996554502 1102 24-25 0.2861444265496331 1102 26-27 0.30442480224389357 1102 28-29 0.24822263057185268 1102 30-31 0.20906133386367998 1102 32-33 0.08614139902113749 1102 34-35 0.08129448790414529 1102 36-37 0.02910509500041769 1102 38-39 0.025341022165171978 1102 40-41 0.027727704920472718 1102 42-43 0.012539239964873161 1102 44-45 -0.026538650570984146 1102 46-47 0.008163851699890756 1102 48-49 -0.028075455615130807 1102 50-51 -0.05230756347008736 1102 52-53 -0.13513150044304467 1102 54-55 -0.0761342588142 1102 56-57 -0.2457678827849108 1102 58-59 -0.29920379209063697 1102 60-61 -0.2139111817560817 1102 62-63 -0.251930473618998 1102 64-65 -0.2273415968880279 1102 66-67 -0.19717320536480543 1102 68-69 -0.24999080338080404 1102 70-71 -0.2573078284160033 1102 72-73 -0.2854911060557761 1102 74-75 -0.2566414300651658 1102 76-77 -0.2947504973272288 1102 78-79 -0.24108916497415223 1102 80-81 -0.2560566599550249 1102 82-83 -0.2628068820294267 1102 84-85 -0.28946199570676256 1102 86-87 -0.3250234626575512 1102 88-89 -0.3021796080155852 1102 90-91 -0.3410931011803733 1102 92-93 -0.3069029642205443 1102 94-95 -0.41671802870211394 1102 96-97 -0.38486992690834754 1102 98-99 -0.2953598792952761 1102 100-101 -0.27973168733916864 1103 1 0.11807996952894939 1103 2 0.0966897908345814 1103 3 0.09153434853193687 1103 4 0.08929725713534964 1103 5 0.10242855735338452 1103 6 0.14488238971785705 1103 7 0.11286803978486404 1103 8 0.12705410452650767 1103 9 0.17278623795233017 1103 10-11 0.15766469243166625 1103 12-13 0.17403163744948813 1103 14-15 0.20786002454835995 1103 16-17 0.23543084271107517 1103 18-19 0.26698670044271466 1103 20-21 0.2065476889718596 1103 22-23 0.2244874153341101 1103 24-25 0.23131680197368354 1103 26-27 0.20445100199802368 1103 28-29 0.14903986952328552 1103 30-31 0.1665799386925002 1103 32-33 0.028443480064950677 1103 34-35 -0.009731804898976293 1103 36-37 -0.02017978435176815 1103 38-39 -0.07151628461308945 1103 40-41 -0.03521683035857137 1103 42-43 -0.030749528776865986 1103 44-45 -0.09188802316468525 1103 46-47 -0.13364801727328768 1103 48-49 -0.07921662539697394 1103 50-51 -0.11456738370534225 1103 52-53 -0.09218283947869566 1103 54-55 -0.10444367038532221 1103 56-57 -0.15244625884139396 1103 58-59 -0.0363562492705114 1103 60-61 -0.20464614213589982 1103 62-63 -0.2154507492208495 1103 64-65 -0.23000663418142508 1103 66-67 -0.22501387736472012 1103 68-69 -0.23939792324270215 1103 70-71 -0.30039438811184027 1103 72-73 -0.28095840121285676 1103 74-75 -0.24105327974972113 1103 76-77 -0.25180050772964435 1103 78-79 -0.18739722035685702 1103 80-81 -0.30045493793024036 1103 82-83 -0.2697197384048664 1103 84-85 -0.3246258037911183 1103 86-87 -0.346456248230016 1103 88-89 -0.34733180161249777 1103 90-91 -0.3850602097593878 1103 92-93 -0.4400274140798537 1103 94-95 -0.4305981484255881 1103 96-97 -0.40146739188060465 1103 98-99 -0.4959950865619476 1103 100-101 -0.5342410071207127 1104 1 0.08421285543672496 1104 2 0.04443606991320337 1104 3 0.10115714559707101 1104 4 0.10993052998937713 1104 5 0.15878931130822593 1104 6 0.17830570278854907 1104 7 0.15823269345212765 1104 8 0.18045950068091798 1104 9 0.22529926294001967 1104 10-11 0.201759669120527 1104 12-13 0.18252841035383938 1104 14-15 0.2810501642625667 1104 16-17 0.2423524081363908 1104 18-19 0.24994335135696133 1104 20-21 0.2756871394420912 1104 22-23 0.29457193578748075 1104 24-25 0.24089929994391213 1104 26-27 0.17651480415188558 1104 28-29 0.15238622733331653 1104 30-31 0.14345360644632166 1104 32-33 0.1126867110944545 1104 34-35 0.050876909693435834 1104 36-37 -0.007090822277262987 1104 38-39 0.0065529751896207244 1104 40-41 -0.012990431746629838 1104 42-43 -0.08664348772111197 1104 44-45 -0.08529109879978591 1104 46-47 -0.08239352995374816 1104 48-49 -0.13962438955754664 1104 50-51 0.05697632507452255 1104 52-53 0.0989463656875742 1104 54-55 0.1291543765631573 1104 56-57 0.05508464388763201 1104 58-59 -0.0078011213690913905 1104 60-61 -0.033657491219472035 1104 62-63 -0.11503923516647774 1104 64-65 -0.11077718012821691 1104 66-67 -0.1271919633913967 1104 68-69 -0.22678001432492323 1104 70-71 -0.27536721943631903 1104 72-73 -0.21236358466435235 1104 74-75 -0.1727477446162311 1104 76-77 -0.17163143684554782 1104 78-79 -0.24271104947329647 1104 80-81 -0.2503825801047981 1104 82-83 -0.33743921637114127 1104 84-85 -0.3426347300917385 1104 86-87 -0.32492018026755076 1104 88-89 -0.3315300598059068 1104 90-91 -0.39577861078940657 1104 92-93 -0.47005712095112173 1104 94-95 -0.4687198658633207 1104 96-97 -0.4483502960643442 1104 98-99 -0.5734141785494202 1104 100-101 -0.6890447852749766 1105 1 0.07210642865177164 1105 2 0.06431301998851069 1105 3 0.1327606636780132 1105 4 0.09545409670993621 1105 5 0.10904625834356096 1105 6 0.14725739481137623 1105 7 0.15410039923775543 1105 8 0.1759335987063153 1105 9 0.15405010929688245 1105 10-11 0.1578844946673783 1105 12-13 0.1593083852049446 1105 14-15 0.2396684607707371 1105 16-17 0.26037759848032493 1105 18-19 0.18171168448301955 1105 20-21 0.29273756849320876 1105 22-23 0.29433457586817724 1105 24-25 0.2208775710268327 1105 26-27 0.191268946118754 1105 28-29 0.16887035727846467 1105 30-31 0.12992087834450672 1105 32-33 0.016419248506878148 1105 34-35 -0.1228913474332387 1105 36-37 -0.06160729341821991 1105 38-39 -0.047636111297684636 1105 40-41 -0.0394929397265642 1105 42-43 -0.08696171790254681 1105 44-45 -0.11586555056781123 1105 46-47 -0.13230112160209728 1105 48-49 -0.02313649418798036 1105 50-51 -0.008432344383400903 1105 52-53 -0.029407280210534736 1105 54-55 -0.13683765731875752 1105 56-57 -0.10245335580837178 1105 58-59 -0.12020477486713332 1105 60-61 -0.13027427464884056 1105 62-63 -0.11086610855149104 1105 64-65 -0.15414647499203937 1105 66-67 -0.22764871973734557 1105 68-69 -0.18000833031657848 1105 70-71 -0.1883737899292086 1105 72-73 -0.2188398037636894 1105 74-75 -0.1962751873057833 1105 76-77 -0.2603375817418403 1105 78-79 -0.319784381977545 1105 80-81 -0.3388332979536024 1105 82-83 -0.36183225759044646 1105 84-85 -0.331054840912671 1105 86-87 -0.3450676536332189 1105 88-89 -0.31111669220375404 1105 90-91 -0.2952037790244262 1105 92-93 -0.3796168426551638 1105 94-95 -0.41525080452490215 1105 96-97 -0.44161978990839756 1105 98-99 -0.5413840275178998 1105 100-101 -0.6641854877857227 1106 1 0.02927774232109215 1106 2 0.05921577590990523 1106 3 0.08940547396463927 1106 4 0.08277800153717152 1106 5 0.10700124092570462 1106 6 0.07575186010344481 1106 7 0.08186069191149414 1106 8 0.09572048218826978 1106 9 0.16494238760594016 1106 10-11 0.16330154802384556 1106 12-13 0.17207399066029438 1106 14-15 0.18283921626173338 1106 16-17 0.20312376857003756 1106 18-19 0.18718596793000586 1106 20-21 0.20603334247805805 1106 22-23 0.14967530002049045 1106 24-25 0.13239625318799852 1106 26-27 0.0985159501134234 1106 28-29 0.10682191009020414 1106 30-31 0.07706677136256701 1106 32-33 0.00427221223201002 1106 34-35 -0.027615889794262216 1106 36-37 -0.03693510549370416 1106 38-39 -0.010913223296505237 1106 40-41 -0.04451439260420642 1106 42-43 -0.001128283693610399 1106 44-45 -0.06219983213178182 1106 46-47 -0.007295909696090064 1106 48-49 0.020188062968138354 1106 50-51 0.09261487884758424 1106 52-53 0.06548740566285005 1106 54-55 0.1210626170628828 1106 56-57 0.14296645481449843 1106 58-59 0.06528985182589508 1106 60-61 -0.03453983302891572 1106 62-63 0.03833421694044148 1106 64-65 0.005908703961281958 1106 66-67 0.010700888192822333 1106 68-69 -0.02532191893243052 1106 70-71 -0.09868431865185556 1106 72-73 -0.032425359722747515 1106 74-75 0.01081983078457327 1106 76-77 0.06488636209062193 1106 78-79 0.041829358624209334 1106 80-81 0.0820316766929956 1106 82-83 0.005930590598502761 1106 84-85 -0.014265442265745776 1106 86-87 0.004568555327228552 1106 88-89 -0.06722228878205527 1106 90-91 -0.07836831385340659 1106 92-93 -0.10133498950314035 1106 94-95 -0.15544976483371897 1106 96-97 -0.18964168663868008 1106 98-99 -0.24112834149656948 1106 100-101 -0.3816180356343821 1107 1 -0.07059904333427625 1107 2 -0.03758037438689854 1107 3 -0.011221241706763863 1107 4 -0.042148911792352806 1107 5 -5.449777584658477E-4 1107 6 0.03198924569430517 1107 7 0.028425666296833185 1107 8 -0.020843161703538726 1107 9 0.03235183155543808 1107 10-11 -0.018002250481949034 1107 12-13 -0.02317253914647921 1107 14-15 0.015950889373307575 1107 16-17 0.05058797563314954 1107 18-19 0.10385524236399135 1107 20-21 0.09943966136436444 1107 22-23 0.09123723555860863 1107 24-25 0.04988727235667767 1107 26-27 0.02476414995568632 1107 28-29 0.007789040513692669 1107 30-31 -0.009891635280958155 1107 32-33 -0.07836165404380324 1107 34-35 -0.12035265111756388 1107 36-37 -0.13734797360135076 1107 38-39 -0.14921884511063865 1107 40-41 -0.18177111718460282 1107 42-43 -0.1717426204532373 1107 44-45 -0.16771043829399446 1107 46-47 -0.20331322101348093 1107 48-49 0.0427480708872352 1107 50-51 0.18513207680693 1107 52-53 0.1011955102843416 1107 54-55 0.06030481052515313 1107 56-57 0.05907542117004283 1107 58-59 -0.007599586290723437 1107 60-61 0.0010403100470384175 1107 62-63 -0.050518386728953146 1107 64-65 -0.11293925894669599 1107 66-67 -0.13670147753111195 1107 68-69 -0.1365289320170362 1107 70-71 -0.14986114549929752 1107 72-73 -0.20924039238370185 1107 74-75 -0.17226330590181504 1107 76-77 -0.15814987735989305 1107 78-79 -0.164881395275998 1107 80-81 -0.13928239322284242 1107 82-83 -0.18150088632382477 1107 84-85 -0.223203303960414 1107 86-87 -0.28581160392752736 1107 88-89 -0.2060098685717513 1107 90-91 -0.22457591767713936 1107 92-93 -0.24780247596669724 1107 94-95 -0.22596314374443693 1107 96-97 -0.19835839902110664 1107 98-99 -0.22762775980479688 1107 100-101 -0.3547386815943092 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0.601854108220806 2213 64-65 0.6704516904596645 2213 66-67 0.679553907248966 2213 68-69 0.7517830472229363 2213 70-71 0.7886400171968901 2213 72-73 0.838634143479478 2213 74-75 0.8068715807328282 2213 76-77 0.7277589387120749 2213 78-79 0.7253771534650575 2213 80-81 0.7109865038116396 2213 82-83 0.7623162264230139 2213 84-85 0.7833386809691412 2213 86-87 0.7764891864679875 2213 88-89 0.8282999180987716 2213 90-91 0.8407653083775628 2213 92-93 0.8471958488785241 2213 94-95 0.8094051028542069 2213 96-97 0.8396937032485816 2213 98-99 0.8256949741270958 2213 100-101 0.8504482914505687 2214 1 -0.1604502869331128 2214 2 -0.11705701842377891 2214 3 -0.18856521701405882 2214 4 -0.14095484645957157 2214 5 -0.17025444095713738 2214 6 -0.17401759780164383 2214 7 -0.12943484486087442 2214 8 -0.16335413234197915 2214 9 -0.16875579372028682 2214 10-11 -0.2183268878468212 2214 12-13 -0.1973469547602491 2214 14-15 -0.26049068759262894 2214 16-17 -0.24933595088111105 2214 18-19 -0.26016116098136877 2214 20-21 -0.3037740536874338 2214 22-23 -0.3219708675147359 2214 24-25 -0.25781378456593274 2214 26-27 -0.2572205807862673 2214 28-29 -0.25511852942168645 2214 30-31 -0.23966487627645705 2214 32-33 -0.10503647886434209 2214 34-35 -0.0954804015715851 2214 36-37 -0.16005676648036626 2214 38-39 -0.12788259017092685 2214 40-41 -0.06740554355466344 2214 42-43 0.003256444217022647 2214 44-45 0.011742627638959391 2214 46-47 0.022907929909763425 2214 48-49 0.20753670585141748 2214 50-51 0.2212874428716063 2214 52-53 0.23638690213294922 2214 54-55 0.33545837997728256 2214 56-57 0.4347189085469907 2214 58-59 0.5529666194662326 2214 60-61 0.5470964532148486 2214 62-63 0.5562050362661353 2214 64-65 0.5530547627373608 2214 66-67 0.5346815438957293 2214 68-69 0.5714417337039066 2214 70-71 0.6481842881429571 2214 72-73 0.6642863658413525 2214 74-75 0.7119882159576179 2214 76-77 0.7575413702920386 2214 78-79 0.6770858370053503 2214 80-81 0.7132507330388762 2214 82-83 0.6847791708871789 2214 84-85 0.755872668197938 2214 86-87 0.7680767371872292 2214 88-89 0.6624996445111719 2214 90-91 0.6665993826026835 2214 92-93 0.7615324071560572 2214 94-95 0.7424808183123517 2214 96-97 0.7340916582678396 2214 98-99 0.8042440298707234 2214 100-101 0.8541660667630175 2215 1 -0.2405597636034571 2215 2 -0.21745920322207013 2215 3 -0.23793286653606316 2215 4 -0.21423486729409547 2215 5 -0.25412844366675813 2215 6 -0.26049063341610434 2215 7 -0.23481319857995686 2215 8 -0.1974789020349519 2215 9 -0.275967681131867 2215 10-11 -0.2630538021955715 2215 12-13 -0.2976464864074586 2215 14-15 -0.4210403395613511 2215 16-17 -0.40126356857179957 2215 18-19 -0.4017312082406903 2215 20-21 -0.4145954979836972 2215 22-23 -0.43730021628031324 2215 24-25 -0.36482085155515165 2215 26-27 -0.3483086794520247 2215 28-29 -0.35905942982940786 2215 30-31 -0.3673542209853835 2215 32-33 -0.2807010740928959 2215 34-35 -0.2655264064381626 2215 36-37 -0.27495500734812595 2215 38-39 -0.273356920169185 2215 40-41 -0.26948641656935735 2215 42-43 -0.2145125688780567 2215 44-45 -0.2093425416132959 2215 46-47 -0.22719304474745172 2215 48-49 -0.22809928254118006 2215 50-51 -0.1892708665917766 2215 52-53 -0.2718211448846688 2215 54-55 -0.17590884868446466 2215 56-57 -0.07329647387751947 2215 58-59 0.016151745325004185 2215 60-61 0.0357056814681016 2215 62-63 0.14269879686273157 2215 64-65 0.042043259930572674 2215 66-67 0.06748174340681246 2215 68-69 0.07276163943969038 2215 70-71 0.20032380243276648 2215 72-73 0.20091602260172436 2215 74-75 0.18366328746145655 2215 76-77 0.22881173925679477 2215 78-79 0.21369364684294823 2215 80-81 0.24083995117170076 2215 82-83 0.08215161242081948 2215 84-85 0.04229064921364056 2215 86-87 0.04797436142934686 2215 88-89 0.03641547882156715 2215 90-91 0.05246270973795575 2215 92-93 0.01730496841048179 2215 94-95 0.009423482375410686 2215 96-97 -0.012957037136054339 2215 98-99 -0.03973722683000247 2215 100-101 -0.07273067547474454 2216 1 -0.19653664896180345 2216 2 -0.16502825607183524 2216 3 -0.27774306855753395 2216 4 -0.20872289844496095 2216 5 -0.22286998433084904 2216 6 -0.3661101706085148 2216 7 -0.25894918118465426 2216 8 -0.273416102015851 2216 9 -0.32761955566645895 2216 10-11 -0.3020226776997319 2216 12-13 -0.314688509810523 2216 14-15 -0.40286275462468524 2216 16-17 -0.4548989438030091 2216 18-19 -0.4195241747773011 2216 20-21 -0.5233529929424492 2216 22-23 -0.4889535162954104 2216 24-25 -0.5246578272683635 2216 26-27 -0.4608842447678967 2216 28-29 -0.45012140568319126 2216 30-31 -0.467462509988259 2216 32-33 -0.4147798862178149 2216 34-35 -0.44319619878762495 2216 36-37 -0.461867124336365 2216 38-39 -0.39879187189013265 2216 40-41 -0.5019493356635962 2216 42-43 -0.372203586632331 2216 44-45 -0.3824121658035722 2216 46-47 -0.4410296138819447 2216 48-49 -1.2995951287305658 2216 50-51 -1.7033877010335416 2216 52-53 -1.5801946549902581 2216 54-55 -1.5252773014227756 2216 56-57 -1.632612697801818 2216 58-59 -1.7533204649720204 2216 60-61 -1.7495511517113194 2216 62-63 -1.7330827327269596 2216 64-65 -1.7037983322804777 2216 66-67 -1.8523848696301073 2216 68-69 -1.8150305692164395 2216 70-71 -1.8912109419520462 2216 72-73 -1.9491665721149225 2216 74-75 -2.084279912842071 2216 76-77 -2.0620336427525814 2216 78-79 -2.0881908700708145 2216 80-81 -2.16583963668387 2216 82-83 -2.1832231729354703 2216 84-85 -2.2101985604928345 2216 86-87 -2.264694089780537 2216 88-89 -2.368818838101241 2216 90-91 -2.484184762833621 2216 92-93 -2.5285858424749 2216 94-95 -2.5819387075934657 2216 96-97 -2.67424979274114 2216 98-99 -2.753617114733384 2216 100-101 -2.970100529302009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 28.0 11 323.0 12 1327.0 13 2665.0 14 3949.0 15 4919.0 16 6053.0 17 8483.0 18 17421.0 19 104912.0 20 130048.0 21 91346.0 22 84469.0 23 73745.0 24 64070.0 25 55693.0 26 54101.0 27 57781.0 28 62721.0 29 65198.0 30 63644.0 31 70379.0 32 89741.0 33 123643.0 34 175549.0 35 674794.0 36 1316611.0 37 509090.0 38 492740.0 39 89588.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.849366705064359 67.60238234756272 10.99304080789097 8.555210139481947 2 11.952293427765847 25.049932570451755 54.59154957150758 8.40622443027482 3 12.33415690373125 63.46257293002855 14.643446519758793 9.559823646481405 4 13.016839367287927 23.093608052569685 53.30849719091751 10.581055389224888 5 22.76082065163951 22.94035098959703 12.716999231601257 41.58182912716221 6 22.379535588163186 22.401759975724474 42.25552395050391 12.963180485608431 7 12.6582236240568 32.046988450882324 13.162732794341881 42.132055130718996 8 42.1886283214781 22.082898967800666 22.65374760125667 13.074725109464563 9 12.67528679248473 51.570456647761475 13.015615802407174 22.738640757346616 10-11 47.954599958799236 22.04734884635629 12.824439351448321 17.173611843396152 12-13 32.384692357969705 21.973078458094683 13.171564708117486 32.470664475818126 14-15 32.1091010535561 21.971387713895826 13.480748430166258 32.43876280238182 16-17 31.93524360731843 22.12668034389737 13.37109477021674 32.56698127856746 18-19 32.068834645662285 22.16398794944319 13.875870900112167 31.891306504782357 20-21 26.91059656129326 37.50506667093802 19.012362899555114 16.571973868213608 22-23 15.979857452466728 26.833801180050465 29.80826515852284 27.378076208959968 24-25 11.294371468068737 21.76272540845933 35.53976563615808 31.40313748731386 26-27 11.284605195656923 21.794171025894638 55.58728667146304 11.333937106985399 28-29 11.337296348748914 21.920654264613034 55.52167022390348 11.220379162734574 30-31 11.364993408322288 21.75606254151778 55.53073572733813 11.3482083228218 32-33 11.331512223858091 21.733804784005205 55.61102383014962 11.323659161987088 34-35 11.376038862200344 21.855894312469996 55.48569741640939 11.282369408920276 36-37 11.369375995258798 21.758220465034743 55.5126937070056 11.359709832700862 38-39 11.283059054580336 21.76542837451408 55.635984553716945 11.315528017188639 40-41 11.372601757217142 21.906794611509252 55.43625427190994 11.284349359363675 42-43 11.477149814618798 21.82254660781097 55.349859468011786 11.350444109558444 44-45 11.40150013503707 21.951310126534405 55.192442284444574 11.454747453983948 46-47 11.82668893109817 22.338134601925404 54.046017607766025 11.789158859210396 48-49 12.813683103814807 23.84288581559053 50.59486387507002 12.74856720552464 50-51 13.252030784002534 25.29213167693794 48.34198060004511 13.113856939014411 52-53 13.310517185302452 25.681102952528796 47.779685797437274 13.228694064731478 54-55 13.671124002294071 25.853981546861863 46.67353865196965 13.80135579887441 56-57 13.810917727503083 26.093261058082266 46.14769663793721 13.948124576477442 58-59 13.958485328843665 25.878655942749617 46.19557485476005 13.967283873646668 60-61 14.006158313277991 25.292965925720267 46.86178967053179 13.839086090469943 62-63 13.686807879401886 25.083825317648568 47.568910061752206 13.660456741197342 64-65 13.584439992151387 25.02102306931473 47.826948771785574 13.567588166748312 66-67 13.688253910624592 24.84706551322318 47.87526846125815 13.589412114894078 68-69 13.912477736680827 24.954883825851567 47.63114502091406 13.501493416553542 70-71 14.008939142552427 25.481294807502096 47.022365876154765 13.487400173790705 72-73 14.02673644990881 25.439537875117185 46.93703891069801 13.596686764275997 74-75 14.081016511685387 25.55450328905583 46.75479231752019 13.609687881738594 76-77 14.13483823976315 25.8265876690161 46.30814200533661 13.73043208588414 78-79 14.21237707157369 25.74556852533943 46.21198907559959 13.83006532748729 80-81 14.159124976708872 25.977913059438958 46.10763142452702 13.755330539325156 82-83 14.098924147474614 26.08905943135496 46.05896915496381 13.753047266206616 84-85 14.16308287205917 26.010200702509394 46.063454549964725 13.763261875466712 86-87 14.289486777867891 26.073069422770402 46.01717551796246 13.620268281399248 88-89 14.350494960298061 26.14433741948796 45.75262941575274 13.752538204461235 90-91 14.29930063209404 26.00701125391235 45.67032379330283 14.023364320690785 92-93 14.184776539233496 26.003990601241984 45.68418633602829 14.127046523496228 94-95 14.39926746282921 26.196268260593513 45.35955232653075 14.044911950046519 96-97 14.419778859557445 26.147136568972794 45.25412552707838 14.178959044391378 98-99 14.492814559621204 26.09732635500912 45.22531613579523 14.184542949574444 100-101 14.860940738970898 26.280015715022397 44.53524778968566 14.323795756321047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 419361.0 1 215663.5 2 8132.0 3 3324.5 4 2401.5 5 1889.5 6 1215.0 7 1068.5 8 1033.0 9 988.5 10 976.0 11 1072.0 12 1269.0 13 2708.5 14 5381.0 15 14722.0 16 616882.0 17 624588.5 18 24349.0 19 10058.0 20 10140.0 21 11767.5 22 14070.5 23 16805.0 24 19724.0 25 22680.5 26 25780.0 27 29216.5 28 33028.0 29 36474.5 30 39758.0 31 43416.5 32 47487.0 33 50310.0 34 51670.5 35 54340.5 36 57822.0 37 62995.5 38 68859.0 39 75122.5 40 81969.5 41 88797.5 42 95231.0 43 100453.5 44 103948.5 45 104880.0 46 104998.0 47 105015.0 48 106939.5 49 107064.5 50 102722.5 51 98450.0 52 90672.0 53 81444.5 54 75630.0 55 69722.0 56 62669.5 57 55689.5 58 50612.0 59 46069.5 60 40721.5 61 35745.0 62 31274.5 63 27298.0 64 23763.5 65 20090.0 66 16558.0 67 13355.5 68 10392.5 69 7724.0 70 5557.5 71 3859.5 72 2603.0 73 1745.5 74 1115.0 75 639.5 76 345.0 77 194.0 78 101.5 79 48.0 80 26.0 81 16.0 82 9.5 83 5.5 84 1.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 1.0 93 1.5 94 8.0 95 7.0 96 0.0 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.339192206915055E-4 58-59 1.1123317097739698E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.673990258643819E-5 76-77 0.0016907441988564338 78-79 0.0037819278132314974 80-81 0.007219032796433063 82-83 0.005661768402749507 84-85 0.0039042843013066338 86-87 0.0026139795179688286 88-89 1.0010985387965727E-4 90-91 6.673990258643819E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4495062.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.596620053580136 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07611427608111 22.65023341151235 2 8.89244752114265 4.908029911747485 3 2.7376089142187707 2.266462591829684 4 1.3859126280412917 1.5298601689405702 5 0.904425884263816 1.2479548747325884 6 0.6254943246439015 1.0356917533721075 7 0.5149976650003072 0.9948536422646096 8 0.39234863635265416 0.866199699677158 9 0.3316019698352713 0.8235984213506451 >10 1.956578022727782 9.67215131095057 >50 0.11877668868032068 2.251349883106897 >100 0.051836575280751904 2.5472458553693915 >500 0.004775693308463016 0.9258230579957388 >1k 0.005269730547269249 2.911738967078028 >5k 8.233953980108203E-4 1.7581867050956559 >10k+ 9.880744776129843E-4 43.61061974497649 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 1187528 26.418501012889255 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 403882 8.985015112138608 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 172613 3.8400582683842845 No Hit ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 26903 0.5985011997609821 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA 24941 0.5548533034694516 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23018 0.5120730259115447 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA 21058 0.4684696228884051 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG 13738 0.3056242605774959 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGG 13018 0.28960668395675077 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA 11175 0.24860613713448224 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10891 0.24228809302296608 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC 10469 0.2329000133924738 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 9885 0.21990797902231382 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 8963 0.19939658229408183 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 8697 0.1934789775980843 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 8247 0.18346799221011856 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 7908 0.17592638321785106 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCA 7612 0.16934137949598915 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCC 7291 0.16220020991924028 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGA 7148 0.1590189412292867 No Hit ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 6294 0.1400203156263473 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 5330 0.11857456026190516 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTGGG 4554 0.10131117212621317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001579511027879037 0.0 0.0 0.0 0.0 2 0.001579511027879037 0.0 0.0 0.0 0.0 3 0.0016017576620745164 0.0 0.0 0.0 0.0 4 0.0016684975646609546 0.0 0.0 0.0 0.0 5 0.0017129908330519134 0.0 0.0 0.0 0.0 6 0.0018464706382247899 0.0 0.0 0.0 0.0 7 0.0019132105408112279 0.0 0.0 0.0 0.0 8 0.001957703809202187 0.0 0.0 0.0 0.0 9 0.0020911836143750633 0.0 0.0 0.0 0.0 10-11 0.0022135401024501998 0.0 0.0 0.0 0.0 12-13 0.002458253078600473 0.0 0.0 0.0 0.0 14-15 0.0025917328837733494 0.0 0.0 0.0 0.0 16-17 0.002747459323141705 0.0 0.0 0.0 0.0 18-19 0.0029476790309010195 0.0 0.0 0.0 0.0 20-21 0.0031367754215625944 0.0 0.0 0.0 0.0 22-23 0.0035817081054721825 0.0 0.0 0.0 0.0 24-25 0.003893160984208894 0.0 0.0 0.0 0.0 26-27 0.004148997277456907 0.0 0.0 0.0 0.0 28-29 0.004682916498148412 0.0 0.0 0.0 0.0 30-31 0.00513897249915574 0.0 0.0 0.0 0.0 32-33 0.005417055426599232 0.0 0.0 2.2246634195479396E-5 0.0 34-35 0.005917604695997519 0.0 0.0 2.2246634195479396E-5 0.0 36-37 0.009454819533078744 0.0 0.0 2.2246634195479396E-5 0.0 38-39 0.009666162557935797 0.0 0.0 2.2246634195479396E-5 0.0 40-41 0.009866382265695112 0.0 0.0 2.2246634195479396E-5 0.0 42-43 0.009955368802477028 0.0 0.0 2.2246634195479396E-5 0.0 44-45 0.010122218558943125 0.0 0.0 2.2246634195479396E-5 0.0 46-47 0.010211205095725043 0.0 0.0 2.2246634195479396E-5 0.0 48-49 0.010400301486386617 0.0 0.0 2.2246634195479396E-5 0.0 50-51 0.010478164706070795 0.0 0.0 2.2246634195479396E-5 0.0 52-53 0.011534879830356067 0.0 0.0 2.2246634195479396E-5 0.0 54-55 0.012725074759814214 0.0 0.0 2.2246634195479396E-5 0.0 56-57 0.014493682178354825 0.0 0.0 4.449326839095879E-5 0.0 58-59 0.016384646084970575 0.0 0.0 4.449326839095879E-5 0.0 60-61 0.018976378968743924 0.0 0.0 4.449326839095879E-5 0.0 62-63 0.022335620732261315 0.0 0.0 4.449326839095879E-5 0.0 64-65 0.026606974497793354 0.0 0.0 4.449326839095879E-5 0.0 66-67 0.031100794605280195 0.0 0.0 6.673990258643818E-5 0.0 68-69 0.03739659208260086 0.0 0.0 6.673990258643818E-5 0.0 70-71 0.04467124146452263 0.0 0.0 6.673990258643818E-5 0.0 72-73 0.053603265094007604 0.0 0.0 6.673990258643818E-5 0.0 74-75 0.06507140502177723 0.0 0.0 6.673990258643818E-5 0.0 76-77 0.07874196173489931 0.0 0.0 6.673990258643818E-5 0.0 78-79 0.09610545972447099 0.0 0.0 6.673990258643818E-5 0.0 80-81 0.11657236318431202 0.0 0.0 6.673990258643818E-5 0.0 82-83 0.14017604206571566 0.0 0.0 6.673990258643818E-5 0.0 84-85 0.16900767998305696 0.0 0.0 6.673990258643818E-5 0.0 86-87 0.20335648318087715 0.0 0.0 6.673990258643818E-5 0.0 88-89 0.24144272092353788 0.0 0.0 6.673990258643818E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 136595 0.0 93.64544 1 TATCTCG 136500 0.0 93.57838 2 ATCTCGA 137920 0.0 92.566696 3 TCTCGAG 138050 0.0 92.39007 4 ATATGGA 46100 0.0 92.17497 1 CTCGAGG 138515 0.0 92.09705 5 TCGAGGG 138805 0.0 91.88069 6 AGGGCGC 139885 0.0 91.38863 9 TATGGAT 46350 0.0 91.26789 2 CGAGGGC 139855 0.0 91.23842 7 GAGGGCG 140585 0.0 90.84574 8 ATGGATC 48190 0.0 87.61552 3 GGATCCG 48605 0.0 85.997696 5 TGGATCC 49205 0.0 85.20003 4 GATCCGG 49660 0.0 84.170715 6 ATCCGGC 50560 0.0 82.61606 7 TCCGGCG 51300 0.0 81.52617 8 CCGGCGC 51830 0.0 80.96744 9 CGCCGGA 142975 0.0 45.84129 14-15 CCGGATC 142405 0.0 45.817978 16-17 >>END_MODULE