FastQCFastQC Report
Thu 26 May 2016
SRR1051301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4495062
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130719729.08073348042808No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2521385.609221852779784No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT558731.2429861924040202No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT304380.6771430516420018No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT297890.6627049860491357No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT133920.29792692514586006No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53640.11933094582455148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA84750.076.4980851
ATATCTC77600.071.716411
TATGGAT91050.071.2600862
TATCTCG83100.066.98882
ATGGATC99900.064.947263
GGATCCG106950.060.754855
TGGATCC107650.060.0950474
GATCCGG119900.054.15336
ATCCGGC133050.048.8367587
TCCGGCG134700.048.168018
ATCTCGA119900.046.4284323
CCGGCGC144400.044.932359
TCTCGAG125200.044.387144
CTCGAGG131350.042.3088655
TCGAGGG134850.041.2107476
AGGGCGC143700.038.7057729
CGAGGGC144750.038.3921937
GAGGGCG149750.037.1420338
CCGTCGA144600.025.933416-17
GTCGACT145200.025.8098818-19
Produced by FastQC (version 0.11.5)