##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4495062 Sequences flagged as poor quality 0 Sequence length 101 %GC 29 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65497672779597 34.0 31.0 34.0 31.0 34.0 2 32.788383119076 34.0 31.0 34.0 31.0 34.0 3 32.80969828669772 34.0 31.0 34.0 31.0 34.0 4 36.19925220163815 37.0 35.0 37.0 35.0 37.0 5 35.99134850642772 37.0 35.0 37.0 35.0 37.0 6 35.845241956618175 37.0 35.0 37.0 33.0 37.0 7 35.71266825685608 37.0 35.0 37.0 33.0 37.0 8 35.66257239610933 37.0 35.0 37.0 33.0 37.0 9 37.154610103264424 39.0 37.0 39.0 32.0 39.0 10-11 37.07220534444241 39.0 37.0 39.0 32.0 39.0 12-13 36.99803539973419 39.0 37.0 39.0 32.0 39.0 14-15 38.605020242212454 41.0 38.0 41.0 32.0 41.0 16-17 38.348163607087066 40.0 37.5 41.0 33.0 41.0 18-19 37.18077804043637 37.0 36.0 41.0 33.5 41.0 20-21 36.98853742173078 37.0 35.0 40.0 34.0 41.0 22-23 37.120899444768504 37.0 35.0 40.0 35.0 41.0 24-25 37.18924622174288 37.0 35.0 40.5 35.0 41.0 26-27 37.17849965139524 37.0 35.0 40.0 35.0 41.0 28-29 37.10504304501251 37.0 35.0 40.0 35.0 41.0 30-31 36.95838266524466 36.5 35.0 40.0 35.0 41.0 32-33 36.75030444518896 35.5 35.0 40.0 35.0 41.0 34-35 36.74343512948208 35.0 35.0 40.0 35.0 41.0 36-37 36.79267015671864 35.0 35.0 40.0 35.0 41.0 38-39 36.79834115747458 35.0 35.0 40.0 35.0 41.0 40-41 36.711619439286935 35.0 35.0 40.0 34.0 41.0 42-43 36.65458096017363 35.0 35.0 40.0 34.0 41.0 44-45 36.53471409293131 35.0 35.0 40.0 33.5 41.0 46-47 36.376760098080965 35.0 35.0 40.0 33.0 41.0 48-49 36.11800193189771 35.0 35.0 40.0 33.0 41.0 50-51 35.94808603307362 35.0 35.0 40.0 33.0 41.0 52-53 35.876520056897995 35.0 35.0 40.0 33.0 41.0 54-55 35.7524762728523 35.0 35.0 40.0 32.5 41.0 56-57 35.49566791292311 35.0 35.0 40.0 32.0 41.0 58-59 35.255268559143346 35.0 35.0 40.0 31.5 41.0 60-61 35.01882932871671 35.0 35.0 39.0 31.0 41.0 62-63 34.675015717247064 35.0 35.0 39.0 31.0 41.0 64-65 34.36368931062575 35.0 35.0 38.0 30.0 40.0 66-67 34.05640311524068 35.0 34.5 37.0 30.0 40.0 68-69 33.71337747955423 35.0 34.0 37.0 29.0 40.0 70-71 33.43374296060877 35.0 34.0 36.0 29.0 39.0 72-73 33.12037876229516 35.0 33.5 35.5 29.0 39.0 74-75 32.759531014255195 35.0 33.0 35.0 28.0 38.0 76-77 32.429471940542754 35.0 33.0 35.0 27.0 37.0 78-79 32.06860984342374 35.0 33.0 35.0 26.5 37.0 80-81 31.802701609010064 35.0 33.0 35.0 25.5 36.0 82-83 31.54355546152645 35.0 33.0 35.0 24.5 36.0 84-85 31.251493861486228 35.0 33.0 35.0 24.0 35.5 86-87 30.972014067881602 35.0 32.0 35.0 22.0 35.0 88-89 30.79366613853157 35.0 32.0 35.0 20.0 35.0 90-91 30.454055138727785 35.0 32.0 35.0 18.5 35.0 92-93 30.21263421950576 34.5 32.0 35.0 11.0 35.0 94-95 29.960147935668076 34.0 31.0 35.0 2.0 35.0 96-97 29.701017249595225 34.0 31.0 35.0 2.0 35.0 98-99 29.41911468629354 34.0 31.0 35.0 2.0 35.0 100-101 28.547948726847373 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09734918776231183 1101 2 0.06699455376247698 1101 3 0.09061066377186933 1101 4 0.055111215214914466 1101 5 0.07821654968680747 1101 6 0.06776182189265967 1101 7 0.038457609704330764 1101 8 0.06786169320571389 1101 9 0.06258249429740914 1101 10-11 0.004913187701355071 1101 12-13 -0.04673077410497939 1101 14-15 -0.04053940487575858 1101 16-17 -0.031127930539803117 1101 18-19 -0.046260531865520704 1101 20-21 -0.06945605487891982 1101 22-23 0.024084920032052537 1101 24-25 -0.008422558299876926 1101 26-27 -0.03301668435099714 1101 28-29 -0.030019784775838332 1101 30-31 -0.0024589939746064715 1101 32-33 -0.0029078130999593554 1101 34-35 0.09147785854962365 1101 36-37 0.06294573374070467 1101 38-39 0.05467203888318295 1101 40-41 0.0227392107184059 1101 42-43 -4.587707242507122E-4 1101 44-45 8.775747773839271E-4 1101 46-47 0.035621644286862875 1101 48-49 -0.05680952849755272 1101 50-51 -0.12711916685722002 1101 52-53 -0.10191774501063833 1101 54-55 0.08040227782444731 1101 56-57 0.15591119227560313 1101 58-59 0.19593063776439124 1101 60-61 0.2219548297189391 1101 62-63 0.2366813254742013 1101 64-65 0.2707246617803847 1101 66-67 0.21367974379917598 1101 68-69 0.2357424648590012 1101 70-71 0.20892886438595326 1101 72-73 0.11844019599897848 1101 74-75 0.0737860948217346 1101 76-77 0.017145547158648355 1101 78-79 0.04357166273668156 1101 80-81 0.020675328744829358 1101 82-83 0.009401248251052152 1101 84-85 -0.025945092014417526 1101 86-87 -0.10527147604023668 1101 88-89 -0.20846590772022466 1101 90-91 -0.2864947184810198 1101 92-93 -0.3754889617907011 1101 94-95 -0.5212719214019792 1101 96-97 -0.5561566725377709 1101 98-99 -0.7157447099138352 1101 100-101 -0.8818117817502866 1102 1 0.11545955422616316 1102 2 0.1102233226426037 1102 3 0.13458504861907983 1102 4 0.09791343831832222 1102 5 0.15962585170207433 1102 6 0.20589866078566388 1102 7 0.1558115995649203 1102 8 0.09800272118365427 1102 9 0.08625648237422467 1102 10-11 9.562836024201715E-4 1102 12-13 0.030284060206824392 1102 14-15 0.09946927686740992 1102 16-17 0.13955670507426277 1102 18-19 0.040874814814735316 1102 20-21 0.016763438951834075 1102 22-23 0.03320571982303733 1102 24-25 0.0013429642338493863 1102 26-27 -0.021504056906827884 1102 28-29 0.027102464965643946 1102 30-31 0.03499799154291594 1102 32-33 0.06827016618770188 1102 34-35 0.07315266079051241 1102 36-37 0.09045102703865382 1102 38-39 0.08417562394922129 1102 40-41 0.038030113957319145 1102 42-43 2.6628081052137986E-4 1102 44-45 -0.02479672118833065 1102 46-47 -0.051315250717507865 1102 48-49 -0.04687704936446124 1102 50-51 -0.08686920013411026 1102 52-53 -0.038831450266464174 1102 54-55 0.15460764233178992 1102 56-57 0.0895432849259663 1102 58-59 0.13609595356835769 1102 60-61 0.1316077153970241 1102 62-63 0.13981879524955332 1102 64-65 0.08507940710868667 1102 66-67 0.1253452629479952 1102 68-69 0.023114637077917166 1102 70-71 -0.02885234823203575 1102 72-73 -0.06406662333895952 1102 74-75 -0.11918780260374717 1102 76-77 -0.14970091752555703 1102 78-79 -0.21161599593611413 1102 80-81 -0.2587442167770746 1102 82-83 -0.31402548494861904 1102 84-85 -0.3010169874275732 1102 86-87 -0.4041255216874333 1102 88-89 -0.5735972551578499 1102 90-91 -0.6974537453045144 1102 92-93 -0.7683528138931308 1102 94-95 -0.9487794095471358 1102 96-97 -1.07511730798592 1102 98-99 -1.3460149723258006 1102 100-101 -1.6710455527460297 1103 1 0.11298586244812725 1103 2 0.10218323820226516 1103 3 0.1472983269103736 1103 4 0.06779296567234638 1103 5 0.10310172996381084 1103 6 0.09442235789721565 1103 7 0.10878765203392504 1103 8 0.06998445893007244 1103 9 0.0737810629263933 1103 10-11 0.1274094427048169 1103 12-13 0.05739098054821312 1103 14-15 0.14198710338510523 1103 16-17 0.15352583205928028 1103 18-19 0.052013070982049214 1103 20-21 0.005108199312509498 1103 22-23 -0.04513100911722745 1103 24-25 -0.04204162241038034 1103 26-27 -0.05681057583126403 1103 28-29 -0.10030755569337657 1103 30-31 -0.05128500246753731 1103 32-33 -0.05845019333911239 1103 34-35 -0.06865768974468978 1103 36-37 -0.05220224637979243 1103 38-39 -0.047719605705928814 1103 40-41 -0.042951544138105646 1103 42-43 -0.05947059889723505 1103 44-45 -0.1047335934691418 1103 46-47 -0.07636367158332291 1103 48-49 -0.14640579097260797 1103 50-51 -0.24348579474531107 1103 52-53 -0.12536869331856337 1103 54-55 2.1629624924912605E-4 1103 56-57 -0.014399902002487863 1103 58-59 -0.03218760391656872 1103 60-61 -0.03794992320237611 1103 62-63 -0.06019383011564372 1103 64-65 -0.1035433591490218 1103 66-67 -0.10107145810889051 1103 68-69 -0.12181471904654728 1103 70-71 -0.12049553995919382 1103 72-73 -0.13282133873087076 1103 74-75 -0.16854215049347232 1103 76-77 -0.2055224714087842 1103 78-79 -0.27336397975751225 1103 80-81 -0.39233824358148084 1103 82-83 -0.4175678712404469 1103 84-85 -0.3807196294850961 1103 86-87 -0.5469887861464322 1103 88-89 -0.7534836215118688 1103 90-91 -1.2041835852435128 1103 92-93 -1.1901262052013237 1103 94-95 -1.4413162645490125 1103 96-97 -1.4758785545368625 1103 98-99 -1.7899381087273305 1103 100-101 -2.129253506087469 1104 1 0.15257149645780999 1104 2 0.17626496558430205 1104 3 0.17789886381831366 1104 4 0.1332370606762865 1104 5 0.13480338939401548 1104 6 0.19118671869940584 1104 7 0.16522818661452732 1104 8 0.1444336132247841 1104 9 0.17166262501060459 1104 10-11 0.1368955362468185 1104 12-13 0.19879134105308083 1104 14-15 0.2064731495765244 1104 16-17 0.21498753848306507 1104 18-19 0.13797049538003137 1104 20-21 0.07930745152754781 1104 22-23 0.082868693016934 1104 24-25 0.0317260241136168 1104 26-27 0.007704902666155533 1104 28-29 -0.06435595984581255 1104 30-31 -0.024500956766793536 1104 32-33 -0.07448640570382992 1104 34-35 -0.012551266341489509 1104 36-37 -0.032122600652101596 1104 38-39 -0.013196737110746426 1104 40-41 -0.024734241198800078 1104 42-43 -0.06762239383527913 1104 44-45 -0.027763855125748194 1104 46-47 -0.10689382985214024 1104 48-49 -0.02291161621869975 1104 50-51 -0.051506288896014496 1104 52-53 -0.0525339895775474 1104 54-55 0.13995672919983093 1104 56-57 0.15288695513071815 1104 58-59 0.08122798181118185 1104 60-61 0.01814965310187233 1104 62-63 -0.012053512414340162 1104 64-65 0.031624744624927814 1104 66-67 -0.001148370447431546 1104 68-69 -0.03416122394998666 1104 70-71 -0.02336497859042197 1104 72-73 -0.1704935321986838 1104 74-75 -0.21404957128395097 1104 76-77 -0.2159929120737658 1104 78-79 -0.46725692614867853 1104 80-81 -0.5377212651878587 1104 82-83 -0.45203814321471114 1104 84-85 -0.548062455690939 1104 86-87 -0.6372469040635593 1104 88-89 -0.9281573402135592 1104 90-91 -0.9855829673967271 1104 92-93 -1.1385395824841709 1104 94-95 -1.441064931732246 1104 96-97 -1.723844880981062 1104 98-99 -1.9145751017703354 1104 100-101 -2.1509807137131105 1105 1 0.13482577660538908 1105 2 0.14423225820959829 1105 3 0.16244963832121329 1105 4 0.08429718310439682 1105 5 0.09256154551058415 1105 6 0.06772377185752276 1105 7 0.10880755070689219 1105 8 0.09852445322533754 1105 9 0.10150772443162737 1105 10-11 0.03205928039141526 1105 12-13 0.11904752809449093 1105 14-15 0.22052723350748948 1105 16-17 0.14375531197723745 1105 18-19 0.009518775721062411 1105 20-21 -0.001662680711397968 1105 22-23 -0.0587637393781506 1105 24-25 -0.1826762406682647 1105 26-27 -0.1185575163254029 1105 28-29 -0.11151651892497938 1105 30-31 -0.14195055535183343 1105 32-33 -0.14368571756387638 1105 34-35 -0.0779884355713989 1105 36-37 -0.1345618011384957 1105 38-39 -0.15222556851645663 1105 40-41 -0.199058399332813 1105 42-43 -0.12980772180719669 1105 44-45 -0.17793374831205 1105 46-47 -0.21729507209674637 1105 48-49 -0.22799265920671985 1105 50-51 -0.3464242786430063 1105 52-53 -0.2204880513995917 1105 54-55 -0.2390623259204503 1105 56-57 -0.18519540748309993 1105 58-59 -0.13702613942857056 1105 60-61 -0.2059844029165916 1105 62-63 -0.29856835302288687 1105 64-65 -0.2630533288595771 1105 66-67 -0.2810147842433892 1105 68-69 -0.31161199707107556 1105 70-71 -0.3777177055867824 1105 72-73 -0.35664592479772494 1105 74-75 -0.30161907618245465 1105 76-77 -0.32494007867825303 1105 78-79 -0.361243854253253 1105 80-81 -0.3521397662034893 1105 82-83 -0.4154393923683557 1105 84-85 -0.4460601480730375 1105 86-87 -0.5359642761427637 1105 88-89 -0.7092313498033036 1105 90-91 -0.9840146163071744 1105 92-93 -1.1054310691704643 1105 94-95 -1.1570010369706765 1105 96-97 -1.3042197997998919 1105 98-99 -1.6078970006496895 1105 100-101 -1.7684311068372338 1106 1 0.1250884566683368 1106 2 0.13779874868021835 1106 3 0.10515155764763051 1106 4 0.054523144448872074 1106 5 0.1232680782144513 1106 6 0.07588380090970048 1106 7 0.13482276274740457 1106 8 0.10364086366797665 1106 9 0.1731137984877691 1106 10-11 0.2136998539028241 1106 12-13 0.23963529659171456 1106 14-15 0.29697506737210233 1106 16-17 0.27905107923705685 1106 18-19 0.12219510627023311 1106 20-21 0.08707122963036795 1106 22-23 0.012424138064716317 1106 24-25 -0.017991745920518554 1106 26-27 -0.009656455114082974 1106 28-29 -0.05155992905826423 1106 30-31 -0.03457305195568239 1106 32-33 -0.025418020621081894 1106 34-35 0.006331091254779153 1106 36-37 -0.029679791965541824 1106 38-39 -0.05391318368276643 1106 40-41 -0.03333370902090138 1106 42-43 -0.06976535405510731 1106 44-45 -0.042712763497199546 1106 46-47 -0.13550947309742867 1106 48-49 -0.16094118575045258 1106 50-51 -0.13376748433508112 1106 52-53 -0.06902646482816266 1106 54-55 0.009082639042084395 1106 56-57 0.0213350434980768 1106 58-59 0.007165208134949808 1106 60-61 -0.15887539674299944 1106 62-63 -0.08584669570402781 1106 64-65 -0.00596570831323362 1106 66-67 -0.09522610517574748 1106 68-69 -0.11209976422329504 1106 70-71 -0.15882837957029494 1106 72-73 -0.25906004603674404 1106 74-75 -0.20606072973529166 1106 76-77 -0.12918966508829755 1106 78-79 -0.20628897927174705 1106 80-81 -0.2776706744873927 1106 82-83 -0.23521390177668522 1106 84-85 -0.24868567094489435 1106 86-87 -0.29423503297876863 1106 88-89 -0.32057185202928906 1106 90-91 -0.4999327860202847 1106 92-93 -0.5724061461721703 1106 94-95 -0.6153235429895858 1106 96-97 -0.7571014458206875 1106 98-99 -0.935456719005014 1106 100-101 -1.2969100709120696 1107 1 0.09638670967711249 1107 2 0.08379297327391555 1107 3 0.10259243317623401 1107 4 0.05903230409851545 1107 5 0.02596900136408209 1107 6 0.09217766328983146 1107 7 0.070890611530956 1107 8 0.06395283779040994 1107 9 0.1033084656568306 1107 10-11 0.11724458669183235 1107 12-13 0.14906060853922298 1107 14-15 0.13623902770849128 1107 16-17 0.1428516712534602 1107 18-19 0.020085049974390756 1107 20-21 -0.06634493490803806 1107 22-23 -0.07957854208144965 1107 24-25 -0.0635935068553124 1107 26-27 -0.08239553681809753 1107 28-29 -0.13261800137537705 1107 30-31 -0.11550189609741324 1107 32-33 -0.07882306223360303 1107 34-35 -0.05036526138347597 1107 36-37 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2214 12-13 0.08981454276995748 2214 14-15 0.034346465918275726 2214 16-17 -0.034211627186877536 2214 18-19 -0.11439295808504113 2214 20-21 -0.12150197287463271 2214 22-23 -0.10846916306245191 2214 24-25 -0.13222391130748434 2214 26-27 -0.08775717816590856 2214 28-29 -0.03525539741738015 2214 30-31 -0.06816497473526084 2214 32-33 -0.1233658613637374 2214 34-35 -0.14131216583174933 2214 36-37 -0.14339260648338836 2214 38-39 -0.1719130172179959 2214 40-41 -0.10698577711934121 2214 42-43 -0.14058031775584823 2214 44-45 -0.15549267999070793 2214 46-47 -0.0893578450045851 2214 48-49 -0.08216187027733923 2214 50-51 -0.049441203033175896 2214 52-53 -0.06434479324929754 2214 54-55 -0.2201896610266587 2214 56-57 -0.2537586400252181 2214 58-59 -0.2865436876080878 2214 60-61 -0.24271837317168377 2214 62-63 -0.12666005005824843 2214 64-65 -0.14893040952538428 2214 66-67 -0.05681413747232256 2214 68-69 0.02975459408424541 2214 70-71 -0.011141242144120156 2214 72-73 0.022641806250568663 2214 74-75 0.022147872830970528 2214 76-77 -0.012869015938171913 2214 78-79 -0.006512001598871109 2214 80-81 0.012696653245608758 2214 82-83 0.07181757601537342 2214 84-85 0.033273382246409966 2214 86-87 0.05968856866500971 2214 88-89 0.10088413330514001 2214 90-91 0.12167048497651933 2214 92-93 0.23178190564814827 2214 94-95 0.2067545032637348 2214 96-97 0.2642345979150704 2214 98-99 0.4540227110939661 2214 100-101 0.5547411903491479 2215 1 -0.2236110377218381 2215 2 -0.2104006659438724 2215 3 -0.20646959313226887 2215 4 -0.0873976517411208 2215 5 -0.1787780737742466 2215 6 -0.14230825091422616 2215 7 -0.1778001110377545 2215 8 -0.09511843356823846 2215 9 -0.18778362348985667 2215 10-11 -0.19722584088000872 2215 12-13 -0.20451530176971033 2215 14-15 -0.2902276736857772 2215 16-17 -0.2821336164459325 2215 18-19 -0.19669260422319468 2215 20-21 -0.16440403752809374 2215 22-23 -0.12150568920914395 2215 24-25 -0.12509796278176566 2215 26-27 -0.06348457556752862 2215 28-29 -0.0077723749411049425 2215 30-31 -0.07676227331423036 2215 32-33 -0.11566608879082452 2215 34-35 -0.14375031068259148 2215 36-37 -0.13662801118415757 2215 38-39 -0.06283207388324286 2215 40-41 -0.0847990741830742 2215 42-43 -0.09397008912024774 2215 44-45 -0.11410080043800974 2215 46-47 -0.14173956595904258 2215 48-49 -0.12176778818732714 2215 50-51 -0.14941069861573197 2215 52-53 -0.14474989686006978 2215 54-55 -0.25018075087449887 2215 56-57 -0.3296846739244259 2215 58-59 -0.2828526530975495 2215 60-61 -0.24642189921024737 2215 62-63 -0.20660680999969827 2215 64-65 -0.28578934854764526 2215 66-67 -0.24259531558419667 2215 68-69 -0.2545672837934845 2215 70-71 -0.16925138879854984 2215 72-73 -0.19712285654177464 2215 74-75 -0.27516337435004345 2215 76-77 -0.2131519923353764 2215 78-79 -0.20907164290296265 2215 80-81 -0.2128248459639117 2215 82-83 -0.03665774023053814 2215 84-85 -0.10187079860971338 2215 86-87 -0.12095849425342209 2215 88-89 -0.030217236063158737 2215 90-91 0.06570963204721281 2215 92-93 0.11480791558906844 2215 94-95 0.07523284786594786 2215 96-97 0.127403725077059 2215 98-99 0.25601140829296654 2215 100-101 0.4010744674971036 2216 1 -0.5311486698133905 2216 2 -0.4942317493541992 2216 3 -0.5425367129908665 2216 4 -0.32371555321839907 2216 5 -0.4327289949553901 2216 6 -0.530379354832462 2216 7 -0.6080927439723283 2216 8 -0.594333714465904 2216 9 -1.147923482134459 2216 10-11 -0.9366644201320966 2216 12-13 -0.9216067259918432 2216 14-15 -1.1197232254342424 2216 16-17 -1.0446221680555752 2216 18-19 -0.5508608399083457 2216 20-21 -0.3886129255416293 2216 22-23 -0.18014535632459427 2216 24-25 -0.13309417195392115 2216 26-27 -0.032424123394022786 2216 28-29 0.051829626148439445 2216 30-31 -0.04147123256219487 2216 32-33 -0.06475522654221066 2216 34-35 -0.11740830195405749 2216 36-37 0.008361492131712112 2216 38-39 0.03659776558944117 2216 40-41 -0.05392250107623653 2216 42-43 -0.03913367528393508 2216 44-45 -0.06534750589516847 2216 46-47 -0.010127864633005856 2216 48-49 0.029454668262459904 2216 50-51 -0.0536717687191981 2216 52-53 -0.08493834221597751 2216 54-55 -0.019279564470828348 2216 56-57 0.028875221717726163 2216 58-59 -0.05618160387024318 2216 60-61 -0.06160458126925761 2216 62-63 -0.14488676661123634 2216 64-65 -0.2801058468769426 2216 66-67 -0.31088141522480583 2216 68-69 -0.315590663178547 2216 70-71 -0.25379746947551496 2216 72-73 -0.26292721836960453 2216 74-75 -0.29195055351496535 2216 76-77 -0.1868332822938683 2216 78-79 -0.1607922335175509 2216 80-81 -0.2691784699828119 2216 82-83 -0.11700047484542608 2216 84-85 -0.09756700368757976 2216 86-87 -0.07191717345386195 2216 88-89 -0.11422064422867706 2216 90-91 -0.007955003547305495 2216 92-93 0.042639646484325056 2216 94-95 0.18895170398752725 2216 96-97 0.18631854160860684 2216 98-99 0.31463239369438156 2216 100-101 0.22576786049788566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 28.0 13 324.0 14 1706.0 15 3840.0 16 6740.0 17 10298.0 18 17800.0 19 34370.0 20 37426.0 21 34823.0 22 33751.0 23 33438.0 24 34336.0 25 37486.0 26 41670.0 27 48366.0 28 55577.0 29 65706.0 30 78151.0 31 102478.0 32 146602.0 33 233758.0 34 434314.0 35 1374204.0 36 430238.0 37 543508.0 38 543628.0 39 110398.0 40 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.63624619256834 52.78911700996197 18.53015040399147 13.044486393478225 2 13.8634127849627 47.33872413773158 27.433014272105698 11.364848805200017 3 13.467800889064488 49.15669683755196 24.123249023039058 13.25225325034449 4 14.435551723202039 46.089753600728976 25.90289077214063 13.571803903928355 5 16.520528526636564 46.195692072767855 21.284111320377782 15.999668080217802 6 16.65734532693876 45.49036253560018 22.355220016987527 15.497072120473533 7 15.022862865962693 47.56819816945795 21.396167616820414 16.012771347758942 8 16.047008917785785 45.20035541222791 23.02778026198526 15.724855408001046 9 15.079836496137316 45.77009171397414 21.94060949548638 17.20946229440217 10-11 17.243377288233177 45.249976529800925 21.268505306489654 16.238140875476244 12-13 16.062559315088425 45.222657663008874 21.708287894582988 17.006495127319713 14-15 15.89038148973251 45.30741733929366 22.11664711187521 16.685554059098628 16-17 15.880303764441958 45.31741720136452 21.95009768497075 16.852181349222768 18-19 15.939168358523197 45.6413281952507 22.618864878838156 15.800638567387947 20-21 14.391314291104326 45.93257000682082 24.380542470826878 15.295573231247978 22-23 14.326064913008988 45.85878904451151 24.34429158040534 15.47085446207416 24-25 13.544618516941478 44.804843626183576 26.44360633957885 15.206931517296091 26-27 13.512060567796395 44.86403079646065 27.95325181276699 13.670656822975968 28-29 13.64707539072876 44.97919049837355 27.73052963451895 13.643204476378745 30-31 13.641458115594402 44.80318625193601 27.8400053213949 13.715350311074687 32-33 13.629489426397235 44.834787595810695 27.898703065719673 13.637019912072404 34-35 13.731634847305777 44.95079266982302 27.65464636527816 13.662926117593038 36-37 13.626842076927973 44.85874722083922 27.737815407217965 13.77659529501484 38-39 13.56346141610505 44.97332851026304 27.813253743774837 13.649956329857075 40-41 13.721023202794532 45.103549183526276 27.44935442492228 13.726073188756907 42-43 13.742925014159981 45.02897846570303 27.40807579517257 13.820020724964415 44-45 13.698476239037415 45.23578317718421 27.231860205710177 13.8338803780682 46-47 13.97472381915978 45.410308022447744 26.62555043734658 13.989417721045893 48-49 14.037982123494627 45.58174058555811 26.139861919590874 14.240415371356391 50-51 14.063643616039112 45.885462758911885 25.86430398512857 14.186589639920427 52-53 14.162752371379971 46.02165665345662 25.578857421766372 14.236733553397038 54-55 14.15409843067793 45.93862109132199 25.580481426062647 14.326799051937437 56-57 14.113932132637993 46.028920179521435 25.6367876572114 14.220360030629168 58-59 14.258157062127285 46.078808256704804 25.400895471519636 14.262139209648275 60-61 14.188736440120293 45.883327082029126 25.608334212075384 14.319602265775199 62-63 14.065056277310525 45.9248170548037 25.78183571216593 14.228290955719855 64-65 14.156739184239871 45.99431020242442 25.529938179765345 14.31901243357036 66-67 14.175491465557505 45.75134361671799 25.61508485833474 14.458080059389767 68-69 14.182281727945039 45.84098861121571 25.579475382884397 14.397254277954858 70-71 14.26348183669617 45.974989980619746 25.248348818369408 14.513179364314677 72-73 14.261517871404553 45.81775140609977 25.24988554096521 14.670845181530472 74-75 14.242837052546244 45.83772472867156 25.299687356752454 14.619750862029745 76-77 14.384762657333757 45.881558474610586 24.98601799040814 14.747660877647517 78-79 14.35886706017091 45.66615854608102 25.084327105074177 14.890647288673895 80-81 14.32702972650653 45.70469984552357 25.15111425593742 14.817156172032481 82-83 14.385237581696256 45.75607726709719 24.901881064822515 14.95680408638404 84-85 14.33821652345532 45.589884331563646 25.0111316979702 15.060767447010836 86-87 14.311887541096628 45.61862708205577 25.032599773995663 15.03688560285194 88-89 14.423389235347301 45.66880637364828 24.77724177576547 15.130562615238949 90-91 14.340767648066807 45.32904915573218 24.947511000859958 15.382672195341057 92-93 14.300644454082883 45.3674627500245 24.98208737357012 15.349805422322499 94-95 14.45446606143926 45.423179565171104 24.755119391567476 15.367234981822154 96-97 14.387886124174434 45.32728748952836 24.79093706314865 15.493889323148558 98-99 14.342199344426266 45.39920409237476 24.919321360606933 15.33927520259204 100-101 14.417765539162753 45.335270125306394 24.500596432262782 15.746367903268075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1407783.0 1 742261.0 2 50601.0 3 19320.5 4 12494.0 5 9998.0 6 8741.0 7 8133.0 8 7861.5 9 7573.0 10 7351.5 11 7233.0 12 7057.0 13 8530.0 14 10381.0 15 11108.0 16 59212.0 17 57371.5 18 6728.0 19 5515.5 20 5534.5 21 5917.0 22 6544.5 23 7525.0 24 8928.5 25 10657.5 26 12757.5 27 15326.5 28 18471.0 29 21717.5 30 25131.0 31 29326.5 32 35005.5 33 39893.0 34 43155.0 35 48180.5 36 54318.5 37 61337.0 38 68081.5 39 74827.5 40 82020.0 41 89716.5 42 97339.0 43 103788.0 44 107841.5 45 108888.0 46 110855.5 47 111428.5 48 112742.0 49 114578.5 50 113057.5 51 109819.5 52 101014.5 53 90961.0 54 84876.5 55 79242.0 56 71832.0 57 63744.0 58 58172.0 59 53318.5 60 47745.0 61 42300.5 62 36995.0 63 32180.0 64 28020.5 65 23824.5 66 19555.0 67 15804.5 68 12250.5 69 8985.0 70 6399.0 71 4347.5 72 2893.5 73 1995.5 74 1245.0 75 645.5 76 340.0 77 174.5 78 75.5 79 30.0 80 12.5 81 5.0 82 2.0 83 1.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12206728183059544 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.003882037667111154 66-67 2.5583629324801307E-4 68-69 0.001546141076585818 70-71 5.227959035937658E-4 72-73 8.898653678191759E-5 74-75 5.561658548869848E-5 76-77 0.0 78-79 0.0014682778569016402 80-81 0.004482696790389098 82-83 0.0029921722992919783 84-85 3.4482283002993064E-4 86-87 3.4482283002993064E-4 88-89 0.0012013182465558873 90-91 0.0016128809791722562 92-93 0.007285772699019502 94-95 0.003726311227742799 96-97 0.0040822573748704695 98-99 0.001512771125292599 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4495062.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.809363858736187 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64845285224031 24.847275285688017 2 9.52687045926635 5.870336368291642 3 2.9771821292930065 2.751752624853456 4 1.4710482097231874 1.8128823818841657 5 0.9545428627214438 1.4704429188172314 6 0.6917765384565459 1.2787917049346837 7 0.5204808842647954 1.122497946138159 8 0.4197514609786736 1.034582039322245 9 0.32816436004690896 0.9099481656739075 >10 2.2666626916017827 12.971027451059047 >50 0.1420702369338211 2.9990973552662483 >100 0.04763732706303319 2.344724171521509 >500 0.002349583522257085 0.5173111322212101 >1k 0.002496432492371679 1.7439909229999682 >5k 7.342448506975526E-5 0.12134235233574377 >10k+ 4.4054691041853157E-4 38.20399717899275 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1307197 29.08073348042808 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252138 5.609221852779784 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 55873 1.2429861924040202 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 30438 0.6771430516420018 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29789 0.6627049860491357 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 13392 0.29792692514586006 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5364 0.11933094582455148 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016684975646609546 0.0 0.0 0.0 0.0 2 0.0016684975646609546 0.0 0.0 0.0 0.0 3 0.0016684975646609546 0.0 0.0 2.2246634195479396E-5 0.0 4 0.0017129908330519134 0.0 0.0 2.2246634195479396E-5 0.0 5 0.0017129908330519134 0.0 0.0 2.2246634195479396E-5 0.0 6 0.0017129908330519134 0.0 0.0 2.2246634195479396E-5 0.0 7 0.0017352374672473928 0.0 0.0 2.2246634195479396E-5 0.0 8 0.0018242240040293105 0.0 0.0 4.449326839095879E-5 0.0 9 0.0018464706382247899 0.0 0.0 4.449326839095879E-5 0.0 10-11 0.0019354571750067075 0.0 0.0 4.449326839095879E-5 0.0 12-13 0.002057813663081844 0.0 0.0 4.449326839095879E-5 0.0 14-15 0.0021134302485705425 0.0 0.0 4.449326839095879E-5 0.0 16-17 0.002246910053743419 0.0 0.0 4.449326839095879E-5 0.0 18-19 0.002458253078600473 0.0 0.0 4.449326839095879E-5 0.0 20-21 0.002613979517968829 0.0 0.0 4.449326839095879E-5 0.0 22-23 0.0029143090796078007 0.0 0.0 4.449326839095879E-5 0.0 24-25 0.003114528787367115 0.0 0.0 4.449326839095879E-5 0.0 26-27 0.0033369951293219092 0.0 0.0 4.449326839095879E-5 0.0 28-29 0.003859791032915675 0.0 0.0 4.449326839095879E-5 0.0 30-31 0.0042936003997275235 0.0 0.0 5.561658548869849E-5 0.0 32-33 0.004460450156193618 0.0 0.0 6.673990258643818E-5 0.0 34-35 0.004972122742689645 0.0 0.0 6.673990258643818E-5 0.0 36-37 0.008531584213966348 0.0 0.0 6.673990258643818E-5 0.0 38-39 0.008754050555921142 0.0 0.0 6.673990258643818E-5 0.0 40-41 0.008932023629484977 0.0 0.0 6.673990258643818E-5 0.0 42-43 0.009009886849169155 0.0 0.0 6.673990258643818E-5 0.0 44-45 0.00915448997143977 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 46-47 0.009232353191123949 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 48-49 0.009388079630492305 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 50-51 0.009454819533078742 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 52-53 0.010544904608657233 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 54-55 0.01180183944070182 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 56-57 0.013537076907949211 2.2246634195479396E-5 0.0 6.673990258643818E-5 0.0 58-59 0.01546141076585818 2.2246634195479396E-5 0.0 7.786321968417788E-5 0.0 60-61 0.01810876023512023 2.2246634195479396E-5 0.0 8.898653678191759E-5 0.0 62-63 0.02145687868153988 2.2246634195479396E-5 0.0 8.898653678191759E-5 0.0 64-65 0.025828342300951577 2.2246634195479396E-5 0.0 8.898653678191759E-5 0.0 66-67 0.030377778993927115 2.2246634195479396E-5 0.0 1.1123317097739698E-4 0.0 68-69 0.036595713251563605 2.2246634195479396E-5 0.0 1.2235648807513668E-4 0.0 70-71 0.043792499413801186 2.2246634195479396E-5 0.0 1.3347980517287636E-4 0.0 72-73 0.05285800284845904 2.2246634195479396E-5 0.0 1.3347980517287636E-4 0.0 74-75 0.06435951272752188 2.2246634195479396E-5 0.0 1.3347980517287636E-4 0.0 76-77 0.07801894612354623 2.2246634195479396E-5 0.0 1.3347980517287636E-4 0.0 78-79 0.09519334772245633 2.2246634195479396E-5 0.0 1.5572643936835576E-4 0.0 80-81 0.11525981176677874 2.2246634195479396E-5 0.0 1.7797307356383517E-4 0.0 82-83 0.13860765435493438 2.2246634195479396E-5 0.0 1.7797307356383517E-4 0.0 84-85 0.16715008602773446 2.2246634195479396E-5 0.0 1.8909639066157485E-4 0.0 86-87 0.20107620317584052 2.2246634195479396E-5 0.0 2.0021970775931455E-4 0.0 88-89 0.2388176180884713 2.2246634195479396E-5 0.0 2.0021970775931455E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATGGA 8475 0.0 76.498085 1 ATATCTC 7760 0.0 71.71641 1 TATGGAT 9105 0.0 71.260086 2 TATCTCG 8310 0.0 66.9888 2 ATGGATC 9990 0.0 64.94726 3 GGATCCG 10695 0.0 60.75485 5 TGGATCC 10765 0.0 60.095047 4 GATCCGG 11990 0.0 54.1533 6 ATCCGGC 13305 0.0 48.836758 7 TCCGGCG 13470 0.0 48.16801 8 ATCTCGA 11990 0.0 46.428432 3 CCGGCGC 14440 0.0 44.93235 9 TCTCGAG 12520 0.0 44.38714 4 CTCGAGG 13135 0.0 42.308865 5 TCGAGGG 13485 0.0 41.210747 6 AGGGCGC 14370 0.0 38.705772 9 CGAGGGC 14475 0.0 38.392193 7 GAGGGCG 14975 0.0 37.142033 8 CCGTCGA 14460 0.0 25.9334 16-17 GTCGACT 14520 0.0 25.80988 18-19 >>END_MODULE