##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051300_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5011656 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.984985402030787 33.0 31.0 34.0 30.0 34.0 2 32.24611405890588 34.0 31.0 34.0 30.0 34.0 3 32.31096108751279 34.0 31.0 34.0 30.0 34.0 4 35.731490150161946 37.0 35.0 37.0 33.0 37.0 5 35.699632217374855 37.0 35.0 37.0 33.0 37.0 6 35.66133749004321 37.0 35.0 37.0 33.0 37.0 7 35.655153705681315 37.0 35.0 37.0 33.0 37.0 8 35.63090503418431 37.0 35.0 37.0 33.0 37.0 9 37.12118469424079 39.0 37.0 39.0 33.0 39.0 10-11 37.073761247779174 39.0 37.0 39.0 33.0 39.0 12-13 37.05089525298624 39.0 37.0 39.0 33.0 39.0 14-15 38.43709444542881 41.0 38.0 41.0 33.0 41.0 16-17 38.409398510193036 40.5 38.0 41.0 33.0 41.0 18-19 38.33370217349315 40.0 38.0 41.0 33.0 41.0 20-21 38.09239081852385 40.0 38.0 41.0 33.0 41.0 22-23 38.16036884814121 40.0 38.0 41.0 33.0 41.0 24-25 38.25913440587303 40.0 38.0 41.0 33.0 41.0 26-27 38.30435129625816 40.0 38.0 41.0 33.0 41.0 28-29 38.14975808395469 40.0 37.5 41.0 33.0 41.0 30-31 37.8113771176633 39.5 37.0 40.5 33.0 41.0 32-33 36.66013369632712 37.0 35.0 40.0 33.0 41.0 34-35 36.24344697640859 36.0 35.0 40.0 32.0 41.0 36-37 36.10035215904683 36.0 35.0 40.0 32.0 41.0 38-39 36.065843006782586 35.5 35.0 40.0 32.0 41.0 40-41 36.084075503186966 35.5 35.0 40.0 32.0 41.0 42-43 36.06826296537512 36.0 35.0 40.0 32.0 41.0 44-45 35.87837882328715 35.0 35.0 40.0 31.0 41.0 46-47 35.43367022796457 35.0 35.0 40.0 29.5 41.0 48-49 34.214932948310896 35.0 33.5 40.0 23.0 41.0 50-51 33.91713846680618 35.0 33.0 40.0 22.0 41.0 52-53 33.91198897131008 35.0 33.0 40.0 23.0 41.0 54-55 33.49671555669424 35.0 33.0 40.0 21.0 41.0 56-57 33.0275889845592 35.0 33.0 40.0 18.5 41.0 58-59 32.684674087766595 35.0 32.0 39.0 18.0 41.0 60-61 32.38652792609868 35.0 32.0 39.0 16.5 41.0 62-63 32.029937609444865 35.0 32.0 39.0 13.5 41.0 64-65 31.66262249444096 35.0 31.5 38.0 9.0 40.5 66-67 31.291734109444064 35.0 31.0 37.5 7.0 40.0 68-69 30.871531086730613 35.0 31.0 37.0 2.0 40.0 70-71 30.42190076493678 35.0 31.0 36.0 2.0 39.0 72-73 30.016085102409264 35.0 30.0 36.0 2.0 39.0 74-75 29.656918691147197 35.0 30.0 35.0 2.0 38.5 76-77 29.285970645231835 35.0 30.0 35.0 2.0 37.0 78-79 28.978047675259432 35.0 29.5 35.0 2.0 37.0 80-81 28.699746251538414 35.0 29.0 35.0 2.0 36.0 82-83 28.473598946136768 34.0 29.0 35.0 2.0 36.0 84-85 28.24929015479115 34.0 29.0 35.0 2.0 36.0 86-87 28.053161170678912 34.0 29.0 35.0 2.0 35.0 88-89 27.889813267311244 34.0 29.0 35.0 2.0 35.0 90-91 27.683418414990975 34.0 28.5 35.0 2.0 35.0 92-93 27.489289069321597 34.0 28.0 35.0 2.0 35.0 94-95 27.27306882196224 34.0 27.0 35.0 2.0 35.0 96-97 27.035773504805597 34.0 27.0 35.0 2.0 35.0 98-99 26.76866828050449 34.0 27.0 35.0 2.0 35.0 100-101 25.7275658983777 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14048724199033558 1101 2 0.18772006244032724 1101 3 0.21077163527959897 1101 4 0.18913758942632342 1101 5 0.20743918549233342 1101 6 0.2436128899680341 1101 7 0.23054064025280496 1101 8 0.25707122727984455 1101 9 0.29778348841819025 1101 10-11 0.3583633628678058 1101 12-13 0.2733212817141464 1101 14-15 0.3362871649141752 1101 16-17 0.2954895000670419 1101 18-19 0.30737551246819805 1101 20-21 0.30809501021465024 1101 22-23 0.28481622133531204 1101 24-25 0.31075265376956906 1101 26-27 0.22207681129798118 1101 28-29 0.18220602582335488 1101 30-31 0.2516576258681482 1101 32-33 0.1581590447104375 1101 34-35 0.09708188431773834 1101 36-37 0.028766886975610362 1101 38-39 0.09562586902458747 1101 40-41 0.06028756461419249 1101 42-43 0.08469824599339404 1101 44-45 0.06496575043973962 1101 46-47 -0.02754190108964849 1101 48-49 -0.04859114496942141 1101 50-51 -0.11194410931507548 1101 52-53 -0.2763057499476673 1101 54-55 -0.4722372189984938 1101 56-57 -0.6146581872747419 1101 58-59 -0.6190180995153511 1101 60-61 -0.7208800172834842 1101 62-63 -0.6869623173730872 1101 64-65 -0.7451778808614229 1101 66-67 -0.6556939828928456 1101 68-69 -0.670442973236721 1101 70-71 -0.7164063605012849 1101 72-73 -0.7672701293981881 1101 74-75 -0.8044484665639757 1101 76-77 -0.7453869979201748 1101 78-79 -0.7280352344949215 1101 80-81 -0.8143992324930238 1101 82-83 -0.8281158203301153 1101 84-85 -0.9021358154786583 1101 86-87 -0.898171525306136 1101 88-89 -0.8814092815339052 1101 90-91 -0.9301862002669417 1101 92-93 -0.8964184317903197 1101 94-95 -0.9781779591525606 1101 96-97 -0.9939453407148271 1101 98-99 -0.959087166659284 1101 100-101 -1.0511538297530016 1102 1 0.15372801157055704 1102 2 0.14321735023882098 1102 3 0.1700952850537476 1102 4 0.17746303576431188 1102 5 0.14691585975717203 1102 6 0.19638586846635775 1102 7 0.166177420304507 1102 8 0.14153105315931214 1102 9 0.19569748838753043 1102 10-11 0.21402945502039472 1102 12-13 0.21873988273794254 1102 14-15 0.23211115578692443 1102 16-17 0.19952532870873085 1102 18-19 0.1890501902069559 1102 20-21 0.1700779020927854 1102 22-23 0.15234271050275083 1102 24-25 0.19068916058627394 1102 26-27 0.1959539514016626 1102 28-29 0.1760251013826064 1102 30-31 0.13955524920615403 1102 32-33 0.06510363904450145 1102 34-35 0.06824108764305237 1102 36-37 0.07092356464562499 1102 38-39 0.050140457732474886 1102 40-41 -0.014613304249166958 1102 42-43 0.010439596129089068 1102 44-45 0.004942021042801059 1102 46-47 -0.03406349304906087 1102 48-49 0.07119582762426546 1102 50-51 -0.0349834164915066 1102 52-53 -0.1360806661049594 1102 54-55 -0.29392001120663735 1102 56-57 -0.3079217607368179 1102 58-59 -0.3103395719862192 1102 60-61 -0.32343361047301755 1102 62-63 -0.37789729639643355 1102 64-65 -0.44073775507084534 1102 66-67 -0.28625880161690276 1102 68-69 -0.34838471766658685 1102 70-71 -0.4291669361979018 1102 72-73 -0.4105731412731579 1102 74-75 -0.420626230233907 1102 76-77 -0.4762248435924654 1102 78-79 -0.5155028981638665 1102 80-81 -0.5277088477999996 1102 82-83 -0.5523977561388058 1102 84-85 -0.5655636916704552 1102 86-87 -0.5761928887731464 1102 88-89 -0.5687226064732513 1102 90-91 -0.5871984276743554 1102 92-93 -0.6887097196121275 1102 94-95 -0.7693941556889285 1102 96-97 -0.8039965375550189 1102 98-99 -0.7707674049856017 1102 100-101 -0.864161661169959 1103 1 0.1018241567464031 1103 2 0.10331402479455676 1103 3 0.11633352830551047 1103 4 0.14321306313934912 1103 5 0.11171201984797108 1103 6 0.14154041626240854 1103 7 0.14270109503107875 1103 8 0.20044427404252474 1103 9 0.2639377385745121 1103 10-11 0.17596991805897488 1103 12-13 0.22651253622441914 1103 14-15 0.27132336789014744 1103 16-17 0.25297955182981013 1103 18-19 0.2776918746757886 1103 20-21 0.21787453255417688 1103 22-23 0.17855425553776172 1103 24-25 0.21127918064478735 1103 26-27 0.18791112909131868 1103 28-29 0.10694367848132913 1103 30-31 0.08245660256319809 1103 32-33 0.11001843645137654 1103 34-35 0.0082212103421071 1103 36-37 0.032962763692921726 1103 38-39 -0.027288901106089725 1103 40-41 0.015750670752581186 1103 42-43 -0.09144496838935368 1103 44-45 -0.17099569225601385 1103 46-47 -0.20895011313504597 1103 48-49 -0.14023087904893572 1103 50-51 -0.26159099746548264 1103 52-53 -0.252513420068297 1103 54-55 -0.3319944166493656 1103 56-57 -0.40556805304093757 1103 58-59 -0.37133333481318687 1103 60-61 -0.5021935667410915 1103 62-63 -0.4862094008425544 1103 64-65 -0.5560966484593415 1103 66-67 -0.531230796212828 1103 68-69 -0.5498089632963747 1103 70-71 -0.6267821208512778 1103 72-73 -0.652865382512406 1103 74-75 -0.6063465717567276 1103 76-77 -0.6929941169775269 1103 78-79 -0.6821911189657683 1103 80-81 -0.7829541111432619 1103 82-83 -0.8203652122856901 1103 84-85 -0.7950579263393891 1103 86-87 -0.8367920831492661 1103 88-89 -0.8796415816136189 1103 90-91 -0.9469678562491914 1103 92-93 -0.9789549426865847 1103 94-95 -1.1819531591661416 1103 96-97 -1.1755286578887336 1103 98-99 -1.2576076180633287 1103 100-101 -1.4426553941825269 1104 1 -0.07813308829891952 1104 2 -0.02956404123145262 1104 3 0.04624850392858093 1104 4 0.08142767690026176 1104 5 0.15316989261177838 1104 6 0.13917689985551362 1104 7 0.09375728026528662 1104 8 0.09512251475091915 1104 9 0.15115599218932374 1104 10-11 0.1140470586227238 1104 12-13 0.13420802215455296 1104 14-15 0.17812177022307907 1104 16-17 0.15310218584475876 1104 18-19 0.1634932537439866 1104 20-21 0.19388199086299096 1104 22-23 0.1565030043901814 1104 24-25 0.10002656866380022 1104 26-27 0.13882589910467402 1104 28-29 0.03241438342991643 1104 30-31 0.045192457547933884 1104 32-33 0.008594001805882101 1104 34-35 -0.04955234717190393 1104 36-37 -0.12995757214563497 1104 38-39 -0.13313315417026672 1104 40-41 -0.09511426661725153 1104 42-43 -0.1925851382429542 1104 44-45 -0.23240975742773884 1104 46-47 -0.27410004585914294 1104 48-49 -0.37660866979679497 1104 50-51 -0.2944695630746921 1104 52-53 -0.20574195307580112 1104 54-55 -0.1578275848210211 1104 56-57 -0.22342773674701277 1104 58-59 -0.2602924992727722 1104 60-61 -0.3622208533509621 1104 62-63 -0.3855187875659283 1104 64-65 -0.37887075746636256 1104 66-67 -0.5218284550098353 1104 68-69 -0.6244805712571981 1104 70-71 -0.5473212565988845 1104 72-73 -0.6198914862809879 1104 74-75 -0.630418207616156 1104 76-77 -0.7539114481353835 1104 78-79 -0.7343794534014414 1104 80-81 -0.827979095767418 1104 82-83 -0.9183322579824882 1104 84-85 -0.9151080033103263 1104 86-87 -0.9168364254514998 1104 88-89 -0.91880017149008 1104 90-91 -1.0565820500011363 1104 92-93 -1.1059617937676265 1104 94-95 -1.2453989960339555 1104 96-97 -1.2491737687372684 1104 98-99 -1.3685827564615352 1104 100-101 -1.5461357015105008 1105 1 -0.0581347897872746 1105 2 -0.07532811059485311 1105 3 0.0456650551702964 1105 4 0.07625397845542636 1105 5 0.02522670347846656 1105 6 0.05211559990976866 1105 7 0.06859763028821675 1105 8 0.08250774918472814 1105 9 0.06554990841446084 1105 10-11 0.09866302653737336 1105 12-13 0.1028267312164246 1105 14-15 0.1456474379051258 1105 16-17 0.17731740967429488 1105 18-19 0.17595904093144554 1105 20-21 0.19268631644368384 1105 22-23 0.1862177868102961 1105 24-25 0.08230396175279964 1105 26-27 8.185771299622502E-4 1105 28-29 -0.09527115820223742 1105 30-31 -0.06296420634456013 1105 32-33 0.004958846920779081 1105 34-35 -0.19233052039763265 1105 36-37 -0.20839971861677498 1105 38-39 -0.2330704888331212 1105 40-41 -0.21163579313418523 1105 42-43 -0.35715683919384844 1105 44-45 -0.4179127390171544 1105 46-47 -0.3793278452736857 1105 48-49 -0.24873212294502878 1105 50-51 -0.11507042558874048 1105 52-53 -0.06533234860981452 1105 54-55 -0.03248427892336281 1105 56-57 -0.06937684983933678 1105 58-59 -0.08805625872503953 1105 60-61 -0.08757830429370017 1105 62-63 -0.11134918278273176 1105 64-65 -0.195840243781376 1105 66-67 -0.2655117187518776 1105 68-69 -0.32355965540734033 1105 70-71 -0.3522796249006035 1105 72-73 -0.3320557676905196 1105 74-75 -0.36094985755694964 1105 76-77 -0.3614877843661297 1105 78-79 -0.420476630921204 1105 80-81 -0.47286868099017454 1105 82-83 -0.44883446950512607 1105 84-85 -0.5466110287913892 1105 86-87 -0.6486424642590158 1105 88-89 -0.7023079302809023 1105 90-91 -0.7487116495503194 1105 92-93 -0.8468767822821874 1105 94-95 -0.9104528475122393 1105 96-97 -1.0781704005833603 1105 98-99 -1.2575715847810862 1105 100-101 -1.496588107398935 1106 1 -0.027256619203186006 1106 2 0.01427952428783641 1106 3 0.0816850923899608 1106 4 0.06485794920994437 1106 5 0.026499257785417285 1106 6 0.11537176041962738 1106 7 0.051095968682453474 1106 8 0.08600233589128692 1106 9 0.06339022204555533 1106 10-11 0.037503537826786726 1106 12-13 0.07069155463084087 1106 14-15 0.10278734965645953 1106 16-17 0.13376603306858925 1106 18-19 0.0697642483989398 1106 20-21 0.06788875958292806 1106 22-23 0.06580995208412332 1106 24-25 0.09997835471753547 1106 26-27 0.04004720010097884 1106 28-29 0.03991939029232583 1106 30-31 -0.08648905990970235 1106 32-33 0.007930788991224347 1106 34-35 -0.1069314335988949 1106 36-37 -0.14219803287484467 1106 38-39 -0.08773444181748857 1106 40-41 -0.13782636355681888 1106 42-43 -0.13528620690195226 1106 44-45 -0.30830150242871923 1106 46-47 -0.29912359249506437 1106 48-49 -0.22379006234912424 1106 50-51 -0.11167818264215867 1106 52-53 -0.1149153025632188 1106 54-55 -0.07213566681400607 1106 56-57 -0.00823287123196792 1106 58-59 -0.1878680134426176 1106 60-61 -0.20428243333531526 1106 62-63 -0.26040121749816336 1106 64-65 -0.18767197780404032 1106 66-67 -0.20009824041104807 1106 68-69 -0.26953683889943036 1106 70-71 -0.28114866790354753 1106 72-73 -0.3069010353097106 1106 74-75 -0.365086623660698 1106 76-77 -0.47044029999408465 1106 78-79 -0.4738960623911126 1106 80-81 -0.48169056793099685 1106 82-83 -0.5817252440728069 1106 84-85 -0.5873947306221083 1106 86-87 -0.6341629806491866 1106 88-89 -0.6514881793028664 1106 90-91 -0.6824818469710117 1106 92-93 -0.7731032054926956 1106 94-95 -0.8579614817880561 1106 96-97 -0.978750715297874 1106 98-99 -1.0568906950696118 1106 100-101 -1.3100768048505387 1107 1 -0.07034885733722263 1107 2 -0.11737477947364994 1107 3 -0.050030183621679214 1107 4 -0.0831985466334686 1107 5 -0.1032396926818393 1107 6 -0.02465238035876638 1107 7 -0.007505405984545632 1107 8 -0.050420172976330946 1107 9 0.011716878595187552 1107 10-11 -0.052217730323043554 1107 12-13 -0.07176012823610733 1107 14-15 -0.009237483673288693 1107 16-17 0.014329185322971227 1107 18-19 0.002201467232396226 1107 20-21 0.006042154233931285 1107 22-23 0.05034673630329678 1107 24-25 -0.00897168562048023 1107 26-27 0.011905897732255255 1107 28-29 -0.018688552301085792 1107 30-31 -0.028974202712845454 1107 32-33 -0.03495164038258025 1107 34-35 -0.01351048899241647 1107 36-37 -0.09087563562549406 1107 38-39 -0.13381355395060268 1107 40-41 -0.12749908378395247 1107 42-43 -0.12791772324277417 1107 44-45 -0.2225828928295428 1107 46-47 -0.15331912915121393 1107 48-49 -0.1382248320757924 1107 50-51 -0.08348114412017082 1107 52-53 -0.1049371445673728 1107 54-55 -0.09005432012637726 1107 56-57 -0.1386499053680339 1107 58-59 -0.22292871750243393 1107 60-61 -0.1561459182551772 1107 62-63 -0.2415164769440281 1107 64-65 -0.26543179311395093 1107 66-67 -0.3482775199897965 1107 68-69 -0.3644435214361472 1107 70-71 -0.36450388216207585 1107 72-73 -0.32970168305320513 1107 74-75 -0.3475984614358332 1107 76-77 -0.40716956751338884 1107 78-79 -0.45924560925892166 1107 80-81 -0.5239365105032405 1107 82-83 -0.5929308380487051 1107 84-85 -0.500027717448674 1107 86-87 -0.5356113908643572 1107 88-89 -0.4930614202570993 1107 90-91 -0.5551486732139637 1107 92-93 -0.6478080957882568 1107 94-95 -0.7267719317968329 1107 96-97 -0.7589741598030919 1107 98-99 -0.851239724165886 1107 100-101 -0.9775228958579127 1108 1 -0.05268638485043553 1108 2 -0.04139715764849683 1108 3 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0.5629123451509557 2214 82-83 0.5665740541142021 2214 84-85 0.5893615299429129 2214 86-87 0.5549057899381182 2214 88-89 0.49864301606735495 2214 90-91 0.5624399179420188 2214 92-93 0.6486186050500287 2214 94-95 0.6488304308075001 2214 96-97 0.7250503810401909 2214 98-99 0.7023331032817524 2214 100-101 0.8304105052721198 2215 1 -0.11622046547736531 2215 2 -0.14884838748756835 2215 3 -0.17798827294699038 2215 4 -0.15226818326163993 2215 5 -0.15862081738314515 2215 6 -0.16300659817587615 2215 7 -0.13124641933521985 2215 8 -0.1455002255373472 2215 9 -0.17064606903343105 2215 10-11 -0.15156038138440664 2215 12-13 -0.22104996002586574 2215 14-15 -0.2509862011651407 2215 16-17 -0.3029315077716106 2215 18-19 -0.3134620484115942 2215 20-21 -0.3225806648222047 2215 22-23 -0.2976312475423981 2215 24-25 -0.23266389864022585 2215 26-27 -0.21632471550154975 2215 28-29 -0.2953823486000857 2215 30-31 -0.28163764485453413 2215 32-33 -0.3259152127348983 2215 34-35 -0.2480309277507189 2215 36-37 -0.25089218051927276 2215 38-39 -0.3179443006346361 2215 40-41 -0.2411772350956909 2215 42-43 -0.16174643049014747 2215 44-45 -0.14830468616950299 2215 46-47 -0.20548478041138196 2215 48-49 -0.2146004673320263 2215 50-51 -0.19108295511902185 2215 52-53 -0.17973156352711328 2215 54-55 -0.17116718619189442 2215 56-57 -0.19541606704895287 2215 58-59 -0.19680856003942182 2215 60-61 -0.16388234084479336 2215 62-63 -0.13970071037999077 2215 64-65 -0.12553375959842938 2215 66-67 -0.10972032274706223 2215 68-69 -0.03699376698120105 2215 70-71 0.03101774195449991 2215 72-73 0.06496184374602265 2215 74-75 0.028914436689522205 2215 76-77 -0.01989586405344923 2215 78-79 -0.07309623119381214 2215 80-81 -0.05884604537121518 2215 82-83 -0.047479698205311394 2215 84-85 -0.04095417190406536 2215 86-87 -0.005428963048270674 2215 88-89 -0.023931751657723765 2215 90-91 -0.031988526680024165 2215 92-93 0.03002407691678144 2215 94-95 0.04876053581898532 2215 96-97 -0.04151049492345038 2215 98-99 0.05232157682785754 2215 100-101 0.10739628905201926 2216 1 0.04110510925693234 2216 2 -0.021921634487796382 2216 3 -0.10507793960400846 2216 4 -0.09258168685773427 2216 5 -0.09008929168096813 2216 6 -0.19463099987009969 2216 7 -0.11922452798403782 2216 8 -0.09485050899544945 2216 9 -0.1647477782433029 2216 10-11 -0.09924640258535788 2216 12-13 -0.06236531743942919 2216 14-15 -0.13609567221372032 2216 16-17 -0.2054393638848282 2216 18-19 -0.23206053245016278 2216 20-21 -0.2742168391091724 2216 22-23 -0.2755185199357939 2216 24-25 -0.3013437994879169 2216 26-27 -0.24432385382459643 2216 28-29 -0.150627641249514 2216 30-31 -0.27705275966989973 2216 32-33 -0.47744886278634624 2216 34-35 -0.5090560649118245 2216 36-37 -0.5335824615098872 2216 38-39 -0.5130712121802219 2216 40-41 -0.5641269419071335 2216 42-43 -0.38640176256793524 2216 44-45 -0.35778040559715407 2216 46-47 -0.4113862956295904 2216 48-49 -1.2574037839650316 2216 50-51 -1.6559913442004657 2216 52-53 -1.6167748395826393 2216 54-55 -1.3832032272939756 2216 56-57 -1.460812322390268 2216 58-59 -1.5094253983916346 2216 60-61 -1.5824691277559495 2216 62-63 -1.4655550551882115 2216 64-65 -1.4263643185324568 2216 66-67 -1.4438618422096319 2216 68-69 -1.448496851283874 2216 70-71 -1.5191384998808104 2216 72-73 -1.5307697738409267 2216 74-75 -1.5809781658435718 2216 76-77 -1.6205617827747645 2216 78-79 -1.6111586494897203 2216 80-81 -1.6247651211449323 2216 82-83 -1.6619608734338804 2216 84-85 -1.6216882998569204 2216 86-87 -1.648730047908245 2216 88-89 -1.7722201120326133 2216 90-91 -1.786105566787679 2216 92-93 -1.8208070594414245 2216 94-95 -1.8204133371204776 2216 96-97 -1.9532074965597879 2216 98-99 -2.071720645114098 2216 100-101 -2.102653372857546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 22.0 11 637.0 12 2718.0 13 5749.0 14 8292.0 15 10380.0 16 12088.0 17 15766.0 18 29052.0 19 138580.0 20 160602.0 21 117239.0 22 108718.0 23 91955.0 24 81424.0 25 72730.0 26 71000.0 27 76942.0 28 87410.0 29 95269.0 30 98775.0 31 112063.0 32 142891.0 33 186813.0 34 253661.0 35 547859.0 36 1012948.0 37 758548.0 38 620529.0 39 90948.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.792444653024868 50.79668277311931 18.436001992155887 14.974870581699943 2 17.065496913595027 24.031138609673132 46.52551970845565 12.377844768276194 3 17.209361536386375 49.2026188549254 19.285401871157955 14.302617737530268 4 18.300956809485726 22.380805865366657 43.89064213505476 15.427595190092855 5 27.56903107475852 21.531964683928827 17.525384822900854 33.3736194184118 6 26.999977652097428 21.2206504197415 33.93191791296131 17.847454015199766 7 17.930759812724574 30.414358048517297 17.71021394924153 33.9446681895166 8 34.1195006201543 20.84231240133002 27.045950480240467 17.992236498275222 9 17.574370627193886 37.05433892509781 17.944368089110664 27.42692235859764 10-11 39.32375845429136 20.847919330456836 17.714563808848812 22.113758406402994 12-13 30.441305229249572 20.93907682410764 17.933024533208187 30.686593413434597 14-15 30.124603524264238 21.061012567502637 18.216234314565884 30.598149593667245 16-17 29.974882553790604 21.154255200277113 18.303450995040365 30.56741125089192 18-19 29.98505883085351 21.11664288211322 18.877133626090856 30.02116466094241 20-21 25.017519159335755 29.712115117238692 23.75374526902884 21.51662045439671 22-23 20.732598167152734 25.62087262174419 28.13354507971018 25.512984131392898 24-25 16.187264249581375 20.703226638061352 33.67769056774846 29.431818544608806 26-27 16.178943646571113 20.73692807327558 46.79118040025094 16.29294787990237 28-29 16.213153895638488 20.828464683130683 46.74728273448936 16.21109868674147 30-31 16.23198998494709 20.679232573025764 46.80379100241517 16.284986439611977 32-33 16.217254336690306 20.692032733292148 46.793854167165506 16.29685876285204 34-35 16.292030019618267 20.780706018130534 46.706108719353445 16.221155242897755 36-37 16.234234751946264 20.7097115205034 46.71165578802695 16.344397939523382 38-39 16.161823556924098 20.72469658731565 46.81794799962328 16.295531856136975 40-41 16.25611374763152 20.854494003578857 46.6566340546917 16.23275819409792 42-43 16.35281830995583 20.80063954908318 46.51888517488032 16.327656966080674 44-45 16.317600409924385 20.938727638129993 46.31448168030687 16.429190271638756 46-47 16.71660026147046 21.360973299045266 45.147123026799925 16.77530341268435 48-49 17.653895239417867 22.674870342258128 41.89447559848482 17.77675881983919 50-51 18.17279557894636 24.051521094025606 39.60233902725965 18.17334429976838 52-53 18.250803726353126 24.411162697519543 39.04150644018664 18.296527135940696 54-55 18.590960752294254 24.683737271672275 37.778171526537335 18.947130449496136 56-57 18.7602985538027 24.91630460852426 37.217781215118606 19.105615622554435 58-59 18.905058527560552 24.63266632825557 37.309334479461484 19.152940664722397 60-61 18.858646722759904 24.020253983912703 38.11067639119684 19.010422902130554 62-63 18.564342804055187 23.837968926837753 38.80437923113638 18.793309037970683 64-65 18.49858609609279 23.75827470999606 39.02853667530253 18.71460251860862 66-67 18.549846597611648 23.633625292717618 39.09569012717553 18.720837982495205 68-69 18.779890319686746 23.704839278673557 38.88919151673618 18.626078884903514 70-71 18.826671263949482 24.249808845619093 38.2776870559352 18.645832834496222 72-73 18.871756162035062 24.24579819524724 38.18371612097877 18.698729521738922 74-75 18.93146076495689 24.385676986943437 38.02309815819325 18.659764089906428 76-77 19.057297622167784 24.618379843962774 37.57715785332732 18.74716468054212 78-79 19.067194136492645 24.538910377919937 37.50528163235089 18.888613853236517 80-81 19.008097485108184 24.79487894280716 37.38985163357518 18.80717193850948 82-83 18.924341684184206 24.827944974718168 37.393122548155404 18.85459079294222 84-85 18.95152194259832 24.83546006977788 37.31265864221371 18.900359345410088 86-87 19.024127046142663 25.0502465961766 37.191021470773364 18.734604886907373 88-89 19.1257654909468 25.115655453400304 36.89924262513785 18.859336430515043 90-91 19.058545494851543 24.95278251202523 36.84762870986887 19.141043283254348 92-93 18.947040658816167 24.94245415088346 36.84825933783165 19.262245852468727 94-95 19.08830135188848 25.122544324670326 36.55999134816915 19.229162975272047 96-97 19.03593343198336 25.10279037507762 36.40835484318956 19.452921349749463 98-99 19.161500709545905 25.082996518516037 36.40776621539866 19.347736556539395 100-101 19.44651628124516 25.23043281502162 35.74748546189124 19.575565441841977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 152586.0 1 80495.5 2 6090.5 3 2884.5 4 1982.5 5 1620.0 6 1202.0 7 1117.0 8 1163.0 9 1142.0 10 1281.0 11 1407.5 12 1464.5 13 2793.5 14 5448.0 15 12557.0 16 339375.5 17 341974.5 18 16464.0 19 10092.5 20 11951.0 21 15081.5 22 18781.5 23 22814.5 24 26982.0 25 31315.5 26 35682.5 27 39952.5 28 44689.5 29 49142.5 30 53136.5 31 57363.0 32 62737.5 33 66945.0 34 69002.5 35 72894.5 36 79224.0 37 87812.0 38 96668.5 39 106783.0 40 117355.5 41 127051.5 42 137265.0 43 146213.5 44 151167.0 45 152766.5 46 154837.0 47 155337.5 48 157057.0 49 158185.0 50 153822.0 51 148168.0 52 139239.5 53 129636.0 54 122186.0 55 113806.5 56 104195.5 57 94035.0 58 86080.0 59 78940.0 60 70571.0 61 62543.5 62 55547.0 63 48769.0 64 42102.5 65 35469.5 66 28927.0 67 23071.5 68 17956.5 69 13573.5 70 10054.0 71 7222.5 72 4923.0 73 3212.5 74 2048.0 75 1163.5 76 626.0 77 342.5 78 172.0 79 84.5 80 42.5 81 23.5 82 16.0 83 9.5 84 4.0 85 1.5 86 1.5 87 1.5 88 3.0 89 3.5 90 1.5 91 0.0 92 1.5 93 2.5 94 4.0 95 4.0 96 1.0 97 0.0 98 0.0 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.986045331124084E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.9953484437080277E-5 76-77 0.0019055577637411667 78-79 0.0036814178786413114 80-81 0.006923859099666857 82-83 0.00499834785148861 84-85 0.0036415109097671506 86-87 0.002444301843542334 88-89 5.986045331124084E-5 90-91 1.296976488410218E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5011656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.87128089743512 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27301147293996 31.98057339242552 2 8.99602354705288 6.993739165148664 3 2.6456810502033545 3.0852303380242723 4 1.2623229930723971 1.9627244658803282 5 0.7847111342833203 1.5251363462035938 6 0.587061453423884 1.3691898396057793 7 0.43955805307487084 1.1960329186262102 8 0.36167436013054804 1.1246996516827714 9 0.289569421290685 1.0130340882868156 >10 2.2009497028979603 15.567985411295407 >50 0.10696881449382507 2.8489054538864815 >100 0.045354963664540164 2.9437881227435825 >500 0.002907517343070811 0.7930699294896527 >1k 0.0030632771801828783 2.6137845865429203 >5k 5.191995220648945E-4 1.6046466994420634 >10k+ 6.230394264778735E-4 23.377459590715798 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 675979 13.48813645629309 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 193169 3.8543946352263605 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 147750 2.948127325578611 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA 23667 0.4722391161723789 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA 22441 0.4477761442525185 No Hit ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 18558 0.3702967641833358 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG 16053 0.32031328566844974 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGG 13788 0.27511864341846287 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC 12667 0.2527507873644959 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 11892 0.23728683692575864 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11501 0.22948502451086028 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 10870 0.2168943758310626 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9977 0.19907591422874993 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCC 8862 0.17682777908140543 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 8828 0.1761493606105447 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCA 8568 0.17096145465690382 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 8294 0.16549419992114384 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 8248 0.16457633963703813 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA 8214 0.1638979211661774 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGA 7066 0.14099132103240924 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 5732 0.11437337279334414 No Hit ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 5720 0.11413393098009918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.582324086090506E-4 0.0 0.0 0.0 0.0 2 7.981393774832111E-4 0.0 0.0 0.0 0.0 3 8.380463463573717E-4 0.0 0.0 0.0 0.0 4 9.178602841056928E-4 0.0 0.0 0.0 0.0 5 9.97674221854014E-4 0.0 0.0 0.0 0.0 6 0.0011373486129135758 0.0 0.0 0.0 0.0 7 0.001217162550661897 0.0 0.0 0.0 0.0 8 0.0012770230039731378 0.0 0.0 0.0 0.0 9 0.0014765578483439405 0.0 0.0 0.0 0.0 10-11 0.001715999661588904 0.0 0.0 0.0 0.0 12-13 0.0020352554125821886 0.0 0.0 0.0 0.0 14-15 0.002414371616886714 0.0 0.0 0.0 0.0 16-17 0.002783511078972699 0.0 0.0 0.0 0.0 18-19 0.0032025342521513848 0.0 0.0 0.0 0.0 20-21 0.0036514876519856913 0.0 0.0 0.0 0.0 22-23 0.004120394536257077 0.0 0.0 0.0 0.0 24-25 0.0049783943670515295 0.0 0.0 0.0 0.0 26-27 0.00580646397119036 0.0 0.0 0.0 0.0 28-29 0.006784184708607295 0.0 0.0 0.0 0.0 30-31 0.007781858930461308 0.0 0.0 0.0 0.0 32-33 0.008350533236918097 0.0 0.0 0.0 0.0 34-35 0.009278370263242328 0.0 0.0 0.0 0.0 36-37 0.014476252959101741 0.0 0.0 0.0 0.0 38-39 0.014945159843373128 0.0 0.0 0.0 0.0 40-41 0.015344229532114735 0.0 0.0 0.0 0.0 42-43 0.01576325270529342 0.0 0.0 0.0 0.0 44-45 0.016212206105127726 0.0 0.0 0.0 0.0 46-47 0.01655141534055809 0.0 0.0 0.0 0.0 48-49 0.016860694349332836 0.0 0.0 0.0 0.0 50-51 0.01716997335810758 0.0 0.0 0.0 0.0 52-53 0.018466949846517797 0.0 0.0 0.0 0.0 54-55 0.0198836472415505 0.0 0.0 0.0 0.0 56-57 0.021499879480954 0.0 0.0 0.0 0.0 58-59 0.02369476276903283 0.0 0.0 0.0 0.0 60-61 0.026149041354793706 0.0 0.0 0.0 0.0 62-63 0.028982436144859104 0.0 0.0 0.0 0.0 64-65 0.03226478433475881 0.0 0.0 0.0 0.0 66-67 0.036265457964393406 0.0 0.0 1.9953484437080277E-5 0.0 68-69 0.04120394536257077 0.0 0.0 1.9953484437080277E-5 0.0 70-71 0.04644173502730435 0.0 0.0 1.9953484437080277E-5 0.0 72-73 0.05309622208707063 0.0 0.0 1.9953484437080277E-5 0.0 74-75 0.060399197391042006 0.0 0.0 1.9953484437080277E-5 0.0 76-77 0.06923859099666857 0.0 0.0 1.9953484437080277E-5 0.0 78-79 0.08059212364136725 0.0 0.0 1.9953484437080277E-5 0.0 80-81 0.09372151640096607 0.0 0.0 1.9953484437080277E-5 0.0 82-83 0.10910565290195495 0.0 0.0 1.9953484437080277E-5 0.0 84-85 0.12686425405095642 0.0 0.0 1.9953484437080277E-5 0.0 86-87 0.14805485452313566 0.0 0.0 1.9953484437080277E-5 0.0 88-89 0.17200901258985055 0.0 0.0 1.9953484437080277E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 86560 0.0 92.326324 2 ATATCTC 86825 0.0 92.175835 1 ATATGGA 49940 0.0 90.88033 1 ATCTCGA 87925 0.0 90.81737 3 TCTCGAG 88505 0.0 90.16318 4 TATGGAT 50155 0.0 90.03616 2 CTCGAGG 89015 0.0 89.635925 5 TCGAGGG 89130 0.0 89.498955 6 AGGGCGC 89895 0.0 88.93811 9 CGAGGGC 90580 0.0 88.10821 7 GAGGGCG 90900 0.0 87.939125 8 ATGGATC 52715 0.0 85.48352 3 GGATCCG 53455 0.0 83.58039 5 TGGATCC 54240 0.0 82.58968 4 GATCCGG 54540 0.0 81.987335 6 ATCCGGC 55600 0.0 80.35593 7 TCCGGCG 56160 0.0 79.57157 8 CCGGCGC 57515 0.0 78.06032 9 GGGCGCG 90290 0.0 44.48757 10-11 CGCCGGA 92400 0.0 44.46896 14-15 >>END_MODULE