FastQCFastQC Report
Thu 26 May 2016
SRR1051300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5011656
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74534614.872249811240037No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT572701.1427360537115876No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT427450.8529116922629966No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT301590.6017771371379042No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT173820.3468314664853294No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT95110.18977759048107054No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT52820.10539430479665803No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51030.10182263108242065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA125400.077.839341
TATGGAT132450.073.478432
ATGGATC157050.061.999163
GGATCCG167800.057.6875575
ATATCTC64450.057.3937261
TGGATCC173500.056.093484
TATCTCG67350.054.5849952
GATCCGG187350.051.7692576
ATCCGGC200850.048.360577
TCCGGCG207800.046.628838
CCGGCGC225400.042.9878929
ATCTCGA96650.038.0863843
TCTCGAG104850.035.1983644
CTCGAGG112250.032.7086835
TCGAGGG114450.032.0799456
AGGGCGC120250.030.5326359
CGAGGGC130100.028.3305037
GAGGGCG134150.027.3335788
GTCGACT224900.025.01586518-19
CCGTCGA224850.025.01086416-17
Produced by FastQC (version 0.11.5)