##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051299_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4836029 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.995310822164218 34.0 31.0 34.0 30.0 34.0 2 32.22725029150983 34.0 31.0 34.0 30.0 34.0 3 32.271814126838365 34.0 31.0 34.0 30.0 34.0 4 35.61331910954215 37.0 35.0 37.0 33.0 37.0 5 35.58589661062827 37.0 35.0 37.0 33.0 37.0 6 35.56612543059605 37.0 35.0 37.0 33.0 37.0 7 35.55374419797731 37.0 35.0 37.0 33.0 37.0 8 35.541152668852895 37.0 35.0 37.0 33.0 37.0 9 37.022199825518 39.0 37.0 39.0 33.0 39.0 10-11 37.05321866349437 39.0 37.0 39.0 33.0 39.0 12-13 37.05453224122519 39.0 37.0 39.0 33.0 39.0 14-15 38.39609119382865 41.0 38.0 41.0 33.0 41.0 16-17 38.39865621153223 40.5 38.0 41.0 33.0 41.0 18-19 38.22472414867653 40.0 38.0 41.0 33.0 41.0 20-21 37.98231834424483 40.0 38.0 41.0 32.5 41.0 22-23 37.96805354144899 40.0 37.5 41.0 32.5 41.0 24-25 38.06438484963593 40.0 37.5 41.0 32.5 41.0 26-27 38.16946083243091 40.0 37.5 41.0 33.0 41.0 28-29 38.033577652243196 40.0 37.0 41.0 33.0 41.0 30-31 37.71788361897747 39.5 37.0 40.5 33.0 41.0 32-33 36.531323736892396 36.0 35.0 40.0 33.0 41.0 34-35 36.18115369035215 35.0 35.0 40.0 33.0 41.0 36-37 36.105544859222306 35.0 35.0 40.0 33.0 41.0 38-39 36.10399389664536 35.0 35.0 40.0 33.0 41.0 40-41 36.07867694755346 35.0 35.0 40.0 33.0 41.0 42-43 35.93294353693909 35.0 35.0 40.0 32.0 41.0 44-45 35.853634252400056 35.0 35.0 40.0 32.0 41.0 46-47 35.543525483407976 35.0 35.0 40.0 30.5 41.0 48-49 34.24413759718976 35.0 34.0 40.0 22.0 41.0 50-51 33.78368419626929 35.0 33.0 40.0 21.0 41.0 52-53 33.408894156755466 35.0 33.0 40.0 20.0 41.0 54-55 32.87788917312117 35.0 32.5 40.0 18.0 41.0 56-57 32.39611838555972 35.0 32.0 39.5 16.0 41.0 58-59 31.994776499479222 35.0 32.0 39.0 10.0 41.0 60-61 31.670753525258014 35.0 31.0 39.0 7.5 41.0 62-63 31.34962073221645 35.0 31.0 38.5 7.0 40.5 64-65 31.02225999885443 35.0 31.0 37.5 3.5 40.0 66-67 30.632887333802174 35.0 31.0 37.0 2.0 40.0 68-69 30.27909871921777 35.0 30.0 36.5 2.0 39.5 70-71 29.863879228184942 35.0 30.0 36.0 2.0 39.0 72-73 29.478688816795763 35.0 30.0 35.5 2.0 39.0 74-75 29.13448502893593 35.0 29.0 35.0 2.0 38.0 76-77 28.817504506279843 35.0 29.0 35.0 2.0 37.0 78-79 28.550424118631213 35.0 29.0 35.0 2.0 37.0 80-81 28.242692299818714 35.0 29.0 35.0 2.0 36.0 82-83 28.01086283808472 34.0 29.0 35.0 2.0 36.0 84-85 27.78955647288302 34.0 28.5 35.0 2.0 35.5 86-87 27.576106450147424 34.0 28.0 35.0 2.0 35.0 88-89 27.381496885151023 34.0 27.0 35.0 2.0 35.0 90-91 27.18903577708074 34.0 27.0 35.0 2.0 35.0 92-93 26.95679161560032 34.0 27.0 35.0 2.0 35.0 94-95 26.754527319832036 34.0 26.5 35.0 2.0 35.0 96-97 26.498255386805994 34.0 25.0 35.0 2.0 35.0 98-99 26.223199137143304 34.0 24.5 35.0 2.0 35.0 100-101 25.12278234890651 32.5 20.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07937991030847513 1101 2 0.11763471987766394 1101 3 0.16150854343361942 1101 4 0.2009471548363848 1101 5 0.1958573431265478 1101 6 0.18969090026951818 1101 7 0.1999927780308255 1101 8 0.21355602969014598 1101 9 0.2596137144109534 1101 10-11 0.2470800937590667 1101 12-13 0.18505647783180024 1101 14-15 0.26122386663558217 1101 16-17 0.2597484036344184 1101 18-19 0.26371785714071905 1101 20-21 0.2851560672925828 1101 22-23 0.31624736130802944 1101 24-25 0.2706480972722076 1101 26-27 0.2703605925772763 1101 28-29 0.24607487670631656 1101 30-31 0.2321733487782751 1101 32-33 0.20398354442723132 1101 34-35 0.23979092553289405 1101 36-37 0.13175617391009808 1101 38-39 0.13919469442077315 1101 40-41 0.16162807969665494 1101 42-43 0.15791639252520895 1101 44-45 0.16522588089635803 1101 46-47 0.23024301671162561 1101 48-49 0.23175119575407166 1101 50-51 0.10751543791177909 1101 52-53 -0.1113172516886678 1101 54-55 -0.10385706039191689 1101 56-57 -0.059959027304770984 1101 58-59 -0.16877836573137017 1101 60-61 -0.08524805237479072 1101 62-63 -0.13521694366895431 1101 64-65 -0.21010801939556245 1101 66-67 -0.1658698075331415 1101 68-69 -0.24414500031486241 1101 70-71 -0.331139563814272 1101 72-73 -0.27308251223703905 1101 74-75 -0.3848010778439388 1101 76-77 -0.3882356921821426 1101 78-79 -0.40850097524265294 1101 80-81 -0.4879880169527304 1101 82-83 -0.5015108286390131 1101 84-85 -0.4328421353141181 1101 86-87 -0.4974583588726702 1101 88-89 -0.4653473649870783 1101 90-91 -0.5124715760504692 1101 92-93 -0.48337673548034843 1101 94-95 -0.441259201397731 1101 96-97 -0.4519759686260372 1101 98-99 -0.42720442066631037 1101 100-101 -0.3887689609572824 1102 1 0.021041041797751348 1102 2 0.1321168827437731 1102 3 0.11850316589491428 1102 4 0.12330883580341379 1102 5 0.15817163743004414 1102 6 0.19380237854409899 1102 7 0.1297602688943158 1102 8 0.14050471909084905 1102 9 0.18996660721941794 1102 10-11 0.14134531331846034 1102 12-13 0.16591747814035074 1102 14-15 0.2557260987280472 1102 16-17 0.1959613164606253 1102 18-19 0.1940327265481585 1102 20-21 0.13926148558762463 1102 22-23 0.19874582353870807 1102 24-25 0.18072217953187675 1102 26-27 0.1442230888689977 1102 28-29 0.21508368585696758 1102 30-31 0.16321258377912784 1102 32-33 0.16899649020705 1102 34-35 0.17466983090255184 1102 36-37 0.18456968774368931 1102 38-39 0.22348487079785428 1102 40-41 0.16135668767181954 1102 42-43 0.14741272504934955 1102 44-45 0.0822044780784239 1102 46-47 0.15292122268834873 1102 48-49 0.2312072234731275 1102 50-51 0.17074528422197943 1102 52-53 0.053664661450831375 1102 54-55 -0.04710636200898222 1102 56-57 -0.13013852152224104 1102 58-59 -0.13745474737699936 1102 60-61 -0.1271970923037209 1102 62-63 -0.23680487615049017 1102 64-65 -0.2689164174974046 1102 66-67 -0.2487219071972291 1102 68-69 -0.30966589516391707 1102 70-71 -0.25620698603760417 1102 72-73 -0.2918045615716096 1102 74-75 -0.2314855957052373 1102 76-77 -0.2468188412406107 1102 78-79 -0.3602020471460925 1102 80-81 -0.258836386362745 1102 82-83 -0.2722071440526328 1102 84-85 -0.14231744802688695 1102 86-87 -0.28588908558304027 1102 88-89 -0.31315858355390347 1102 90-91 -0.20833554099606033 1102 92-93 -0.2067887336853289 1102 94-95 -0.15907356085591218 1102 96-97 -0.08490130294480736 1102 98-99 -0.11088867830495275 1102 100-101 -0.12088736741238293 1103 1 0.12134172282905809 1103 2 0.12234517327809158 1103 3 0.20370497600667647 1103 4 0.1829121300481873 1103 5 0.14922727331538255 1103 6 0.1944478460376331 1103 7 0.2211206042070586 1103 8 0.14314111001705498 1103 9 0.27288231342547675 1103 10-11 0.22915932320807997 1103 12-13 0.20700275121136258 1103 14-15 0.22650629112884957 1103 16-17 0.2389417681146142 1103 18-19 0.2737060212529272 1103 20-21 0.274379953356636 1103 22-23 0.2635272048193613 1103 24-25 0.29090336961763796 1103 26-27 0.23484841107268295 1103 28-29 0.20987949943940976 1103 30-31 0.2012768307036339 1103 32-33 0.26824938960098876 1103 34-35 0.24109717953290044 1103 36-37 0.23617994790838281 1103 38-39 0.24760660590656158 1103 40-41 0.2558476160517671 1103 42-43 0.24990880327649734 1103 44-45 0.2615318757089753 1103 46-47 0.25726518762079564 1103 48-49 0.33147027495105164 1103 50-51 0.3220482158378033 1103 52-53 0.294715158231611 1103 54-55 0.18820614784769418 1103 56-57 0.03861183160188375 1103 58-59 0.05073917056444799 1103 60-61 0.023020892559085127 1103 62-63 -0.0819206045445533 1103 64-65 -0.03383063402950626 1103 66-67 -0.033868495661735665 1103 68-69 -0.09829900764431798 1103 70-71 -0.10835256521832548 1103 72-73 -0.16410981808198954 1103 74-75 -0.14929487681094145 1103 76-77 -0.1472630274756206 1103 78-79 -0.14598661152146164 1103 80-81 -0.19059846758983667 1103 82-83 -0.18847262401490994 1103 84-85 -0.2494030293230196 1103 86-87 -0.24473531804188298 1103 88-89 -0.16808063212173252 1103 90-91 -0.1440782272223693 1103 92-93 -0.1405376694666316 1103 94-95 -0.10700042790471542 1103 96-97 -0.07322747861947221 1103 98-99 -0.017490289225424505 1103 100-101 -0.025191132229512192 1104 1 -0.007445051407213299 1104 2 0.016160018446797153 1104 3 0.05107506011592022 1104 4 0.12921471110174565 1104 5 0.07126641482619078 1104 6 0.19055592262569832 1104 7 0.11739320799544117 1104 8 0.13492814512859042 1104 9 0.15825760997041982 1104 10-11 0.15849534713482427 1104 12-13 0.17394314353239082 1104 14-15 0.197200348537109 1104 16-17 0.09966973106377708 1104 18-19 0.22137209234474398 1104 20-21 0.18937827577146749 1104 22-23 0.24048424687183712 1104 24-25 0.23590470676048625 1104 26-27 0.14710992344812013 1104 28-29 0.1473265822454337 1104 30-31 0.19676663972147423 1104 32-33 0.2718329674795541 1104 34-35 0.31569996196468253 1104 36-37 0.30445192513452213 1104 38-39 0.278824198069735 1104 40-41 0.2640306995017454 1104 42-43 0.25578149511827064 1104 44-45 0.2246755427595204 1104 46-47 0.25295557321850026 1104 48-49 0.3175509341858671 1104 50-51 0.3741003215652583 1104 52-53 0.16494251290802708 1104 54-55 0.2531637480442299 1104 56-57 0.23372307576668305 1104 58-59 0.2186158534535778 1104 60-61 0.24875765394324745 1104 62-63 0.22477368053966273 1104 64-65 0.16274110150525445 1104 66-67 0.20265912291723964 1104 68-69 0.13782908579811348 1104 70-71 0.12419428934090604 1104 72-73 0.04146406143568271 1104 74-75 0.11179199724788091 1104 76-77 0.13411331240550695 1104 78-79 0.16229524347784618 1104 80-81 0.1555611091422655 1104 82-83 0.13877028139486214 1104 84-85 0.17010469067026435 1104 86-87 0.16874389386382305 1104 88-89 0.09530347540841433 1104 90-91 0.1219962401662329 1104 92-93 0.17845553749322107 1104 94-95 0.1784678658591723 1104 96-97 0.1902428420126867 1104 98-99 0.287286431823329 1104 100-101 0.2889282060338694 1105 1 -0.05171365963648711 1105 2 -0.011385498345738654 1105 3 0.045914023195102516 1105 4 0.041449778070138166 1105 5 0.0469580777731764 1105 6 0.1615544462321381 1105 7 0.14563704529890487 1105 8 0.18110893112961435 1105 9 0.2110100371189958 1105 10-11 0.15475453042115817 1105 12-13 0.16988301616913049 1105 14-15 0.15959874994447176 1105 16-17 0.14610176460303137 1105 18-19 0.23092019996286695 1105 20-21 0.2045679088150294 1105 22-23 0.19367387458655116 1105 24-25 0.16561348258668573 1105 26-27 0.1392560814622783 1105 28-29 0.11810827551020253 1105 30-31 0.16456459443048033 1105 32-33 0.22065400520931178 1105 34-35 0.1866553979655592 1105 36-37 0.17889232265354593 1105 38-39 0.22754481892069833 1105 40-41 0.20870440494587683 1105 42-43 0.16473212381006874 1105 44-45 0.12068270121586977 1105 46-47 0.1691681702850616 1105 48-49 0.32786487451073043 1105 50-51 0.26755696141676566 1105 52-53 0.22323249269383183 1105 54-55 0.2499656432417865 1105 56-57 0.2665018394829559 1105 58-59 0.30141365180273993 1105 60-61 0.2757600882086564 1105 62-63 0.21388005686221945 1105 64-65 0.24878132831658206 1105 66-67 0.29716315538864535 1105 68-69 0.24522053535246968 1105 70-71 0.20025797654011512 1105 72-73 0.25019456230585746 1105 74-75 0.21097765993327044 1105 76-77 0.19899984473812538 1105 78-79 0.2787342087366511 1105 80-81 0.24599013953070425 1105 82-83 0.20801743046200727 1105 84-85 0.20684316487099608 1105 86-87 0.20114937724042647 1105 88-89 0.19967742029246338 1105 90-91 0.19433921406082888 1105 92-93 0.20419012477444198 1105 94-95 0.2751392301326021 1105 96-97 0.28117701187220945 1105 98-99 0.28422407261104965 1105 100-101 0.208589364126059 1106 1 -0.009027628275980248 1106 2 -0.06017076669797916 1106 3 0.0274218345631283 1106 4 0.006373736939615071 1106 5 0.05176483741197302 1106 6 0.0722091023015139 1106 7 0.049543079624442043 1106 8 0.023824894601091273 1106 9 0.08068207842160291 1106 10-11 0.0689929140531973 1106 12-13 0.014378519683965862 1106 14-15 0.03873324099117781 1106 16-17 0.05699843688303474 1106 18-19 0.09365571388175198 1106 20-21 0.03826583950212381 1106 22-23 0.046778980785035174 1106 24-25 0.07679490366287212 1106 26-27 0.05786450890332162 1106 28-29 0.06901438292891982 1106 30-31 0.028056411420209315 1106 32-33 0.04053900398533017 1106 34-35 0.07807438789809851 1106 36-37 0.06870726758979373 1106 38-39 0.10651163185671209 1106 40-41 0.07568385908399478 1106 42-43 0.06221457618721615 1106 44-45 0.1462731313093073 1106 46-47 0.07797335850733589 1106 48-49 0.15194626530159638 1106 50-51 0.18120200097518335 1106 52-53 0.09882266897810155 1106 54-55 0.02359325508530219 1106 56-57 -0.0018223670463726194 1106 58-59 -0.03445949101622148 1106 60-61 0.0013883040214928144 1106 62-63 -0.04779729285433376 1106 64-65 0.011177604567421895 1106 66-67 0.039004623711164754 1106 68-69 0.024237625665243456 1106 70-71 -0.02440832182339392 1106 72-73 -0.040112635380545214 1106 74-75 -0.05116478784751877 1106 76-77 -0.031427574341670095 1106 78-79 -0.024997679687139396 1106 80-81 -0.06370346570771446 1106 82-83 -0.016708266967469854 1106 84-85 -0.006316024104670248 1106 86-87 -0.035814004311436776 1106 88-89 -0.05094985025010601 1106 90-91 0.02167151428469083 1106 92-93 0.05447002610501528 1106 94-95 -0.014987454226588426 1106 96-97 -0.05520459853210369 1106 98-99 -0.043639895093310344 1106 100-101 0.058518458722740974 1107 1 0.1135545495464001 1107 2 0.025373566435078487 1107 3 0.04081111303155183 1107 4 0.057718559709059036 1107 5 0.14343998395813173 1107 6 0.10079308141389731 1107 7 0.14239414912417914 1107 8 0.10733768169964719 1107 9 0.16584099236818872 1107 10-11 0.10891727985785593 1107 12-13 0.0922344537861548 1107 14-15 0.09768234486621452 1107 16-17 0.19979466773131804 1107 18-19 0.12538674771256098 1107 20-21 0.12115027311801896 1107 22-23 0.18258902870454818 1107 24-25 0.1341869572245642 1107 26-27 0.17013253496524783 1107 28-29 0.18498162552867825 1107 30-31 0.14785119335907382 1107 32-33 0.10474791523379423 1107 34-35 0.07719245976987565 1107 36-37 0.03089992748423498 1107 38-39 0.0013635772647617728 1107 40-41 -0.00586855868606051 1107 42-43 -0.009762193929752527 1107 44-45 0.01843946681036357 1107 46-47 0.011274736983850175 1107 48-49 0.08225178908167408 1107 50-51 0.09888814552920877 1107 52-53 0.12757326863645346 1107 54-55 0.08784279312227739 1107 56-57 -0.027759284556509556 1107 58-59 0.02331483564453407 1107 60-61 -0.028765497580000243 1107 62-63 0.002351340523798484 1107 64-65 -0.05500626070730874 1107 66-67 0.038274383952824564 1107 68-69 0.014045410618166443 1107 70-71 -0.010544913894726449 1107 72-73 -0.10755879544750258 1107 74-75 -0.059479727839047314 1107 76-77 -0.031887945539040174 1107 78-79 -0.056928184851297914 1107 80-81 -0.07779102532114024 1107 82-83 -0.05846297947302048 1107 84-85 -0.05885882061350145 1107 86-87 -0.05297931080712459 1107 88-89 -0.07007320642177817 1107 90-91 -0.09385886355590145 1107 92-93 -0.04316518113377299 1107 94-95 0.030106430938367623 1107 96-97 -0.01478420274228398 1107 98-99 -0.012476415431972754 1107 100-101 -0.025372121822044846 1108 1 -0.0603305854395515 1108 2 -0.03329541478837683 1108 3 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-0.03121662394626412 2214 44-45 0.05003119463030714 2214 46-47 1.8863938676361158E-4 2214 48-49 -0.016613029776522126 2214 50-51 -0.006576363632568416 2214 52-53 0.07825305267027005 2214 54-55 0.15435006014294572 2214 56-57 -0.044606949543123164 2214 58-59 0.27822080149737616 2214 60-61 0.3988172619076984 2214 62-63 0.35280976405803344 2214 64-65 0.39995541936098533 2214 66-67 0.4912005985856567 2214 68-69 0.4490757315839815 2214 70-71 0.3975343820452899 2214 72-73 0.36739877434300894 2214 74-75 0.33051212153672793 2214 76-77 0.36856828653231233 2214 78-79 0.3883353325357639 2214 80-81 0.3356484489596383 2214 82-83 0.3042214309487292 2214 84-85 0.2827327048268202 2214 86-87 0.3165687006901301 2214 88-89 0.22559340448517418 2214 90-91 0.35956842177632353 2214 92-93 0.3956346076432453 2214 94-95 0.2949140006977018 2214 96-97 0.25036871831259333 2214 98-99 0.27316024649334736 2214 100-101 0.234074974705333 2215 1 -0.05088667424343285 2215 2 -0.05132997247191895 2215 3 -0.098615918112408 2215 4 -0.11176298854209676 2215 5 -0.09359107981197212 2215 6 -0.18213762918066578 2215 7 -0.11337105991807306 2215 8 -0.11912971678222561 2215 9 -0.1997374432544774 2215 10-11 -0.16007423957596245 2215 12-13 -0.2263472095281216 2215 14-15 -0.305976461144823 2215 16-17 -0.28191694817812163 2215 18-19 -0.15349443157071363 2215 20-21 -0.17885001327149297 2215 22-23 -0.1594283464652193 2215 24-25 -0.10652976771009293 2215 26-27 -0.15594081609805244 2215 28-29 -0.054236701305249824 2215 30-31 -0.1598948573704959 2215 32-33 -0.15810768439301626 2215 34-35 -0.08574863121705789 2215 36-37 -0.2204190189493076 2215 38-39 -0.21508791268894356 2215 40-41 -0.1753688613588622 2215 42-43 -0.24108067068449657 2215 44-45 -0.24844656603868032 2215 46-47 -0.170986329475042 2215 48-49 -0.3251444082220445 2215 50-51 -0.35169558175335425 2215 52-53 -0.17506612982120373 2215 54-55 -0.40188605165280933 2215 56-57 -0.14814620824057556 2215 58-59 2.626729864232402E-4 2215 60-61 -0.12149569624103762 2215 62-63 -0.4511179410918409 2215 64-65 -0.2879495888054393 2215 66-67 -0.041617365721798194 2215 68-69 -0.013555640284053538 2215 70-71 0.08074256078639408 2215 72-73 0.08924428754376024 2215 74-75 0.06446107692202219 2215 76-77 0.0760266576912052 2215 78-79 0.1144724104133914 2215 80-81 0.17586664331441426 2215 82-83 0.13987297473345706 2215 84-85 0.06629133340677384 2215 86-87 0.014895755942088584 2215 88-89 0.007198226519133044 2215 90-91 -0.0020593735256113632 2215 92-93 -0.03752370054255394 2215 94-95 -0.07071117020090867 2215 96-97 -0.09530897960803486 2215 98-99 -0.1544932423703962 2215 100-101 -0.11874771970105868 2216 1 0.0026023225941784744 2216 2 -0.03987833748349345 2216 3 -0.1690351141480093 2216 4 -0.088986474995977 2216 5 -0.14471147885999613 2216 6 -0.14516582987835136 2216 7 -0.19004318197981718 2216 8 -0.13192676956480653 2216 9 -0.20113984927932194 2216 10-11 -0.17766586739196555 2216 12-13 -0.250620870402507 2216 14-15 -0.3298218626379992 2216 16-17 -0.25198117404452347 2216 18-19 -0.14998313499065574 2216 20-21 -0.2611046998036173 2216 22-23 -0.3663300515478696 2216 24-25 -0.33225356388262384 2216 26-27 -0.25705697689092943 2216 28-29 -0.29399544678172873 2216 30-31 -0.2667048205302791 2216 32-33 -0.5133536662011267 2216 34-35 -0.445065424488007 2216 36-37 -0.5393055967212845 2216 38-39 -0.5742607356928886 2216 40-41 -0.5427950016125038 2216 42-43 -0.5229528057870283 2216 44-45 -0.5859832269456646 2216 46-47 -0.5205640688549593 2216 48-49 -1.064959033462479 2216 50-51 -1.478145488837761 2216 52-53 -1.5059295601997107 2216 54-55 -1.3708165768704248 2216 56-57 -1.3850724952030475 2216 58-59 -1.3316313706350051 2216 60-61 -1.3629067743879446 2216 62-63 -1.3246244777853526 2216 64-65 -1.4176147666491445 2216 66-67 -1.3150129253344858 2216 68-69 -1.4559705980645106 2216 70-71 -1.4880084580249395 2216 72-73 -1.4923399722286774 2216 74-75 -1.4467689951283162 2216 76-77 -1.4485451155295408 2216 78-79 -1.4661041226939027 2216 80-81 -1.340888613752064 2216 82-83 -1.4791541174974938 2216 84-85 -1.4829440853654354 2216 86-87 -1.5112834244445708 2216 88-89 -1.513505649854224 2216 90-91 -1.5676691280328328 2216 92-93 -1.5615104641457265 2216 94-95 -1.7382372680719982 2216 96-97 -1.837159075783827 2216 98-99 -2.012842539798097 2216 100-101 -2.1361130898963445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 49.0 11 680.0 12 2650.0 13 5200.0 14 7438.0 15 9453.0 16 11154.0 17 15209.0 18 30396.0 19 155358.0 20 179678.0 21 120328.0 22 107085.0 23 93437.0 24 84798.0 25 78455.0 26 77084.0 27 83368.0 28 94751.0 29 99582.0 30 96809.0 31 102690.0 32 128162.0 33 166214.0 34 234393.0 35 533100.0 36 980347.0 37 710478.0 38 559667.0 39 68006.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.588725584565355 55.594455699087 16.64535096873902 13.171467747608625 2 15.30218284464382 22.87794386675514 50.56216577692152 11.257707511679522 3 15.565539412604846 53.64723826097817 17.8497068565966 12.937515469820385 4 16.6505411774826 21.209281416633356 48.188048500122726 13.952128905761318 5 27.304861075067993 20.65525248090944 16.094423751387758 35.945462692634806 6 26.85978516671426 20.203890423320455 36.58933807055334 16.34698633941194 7 16.280092613174983 30.85514995877816 16.29907512961564 36.56568229843121 8 36.75925020300747 19.91592688960302 26.922253774739563 16.40256913264995 9 16.000689822166077 40.378376556468126 16.302445663580595 27.318487957785198 10-11 42.799226390081614 19.790824248572537 16.13826137105464 21.27168799029121 12-13 31.77019823495682 19.84634500744309 16.42190317717284 31.96155358042725 14-15 31.382038858741335 19.960695024781696 16.70790228925426 31.949363827222705 16-17 31.22475072006392 20.169864572772415 16.708698396969908 31.896686310193758 18-19 31.216624217927556 20.083285273930326 17.36987102434663 31.330219483795485 20-21 25.48495883709548 30.87304687378839 22.975647995493823 20.666346293622308 22-23 19.877930839537978 25.421084116741234 28.714343937970593 25.98664110575019 24-25 14.503976299563135 19.64864147836996 35.13744437843528 30.709937843631625 26-27 14.481881725688575 19.68242953050943 51.19802838237736 14.637660361424631 28-29 14.573434112988156 19.72999955128474 51.1472842698007 14.549282065926402 30-31 14.52423052053658 19.605248438336496 51.21836531584074 14.652155725286182 32-33 14.493968088280695 19.62063296146487 51.27919001312854 14.606208937125894 34-35 14.576794307891868 19.685738030106933 51.15839876063605 14.579068901365149 36-37 14.540204370155763 19.607905577075737 51.19595023106768 14.655939821700823 38-39 14.466528219743926 19.629534893194396 51.29588759703467 14.608049290027003 40-41 14.58960440477094 19.755929916880152 51.055297641928945 14.599168036419963 42-43 14.634424235255828 19.722327967843036 50.914924207443754 14.728323589457384 44-45 14.669277210703244 19.915296206867243 50.64381334355108 14.771613238878428 46-47 15.102897439200632 20.34369520943733 49.28739054294339 15.266016808418643 48-49 16.260955010815692 22.006092188446345 45.19553129230614 16.537421508431816 50-51 16.815449204295508 23.76912958958683 42.51508830902379 16.900332897093875 52-53 17.089289580356116 24.134811846661798 41.67930961538899 17.096588957593102 54-55 17.38514181780134 24.48592636644652 40.20354303086272 17.92538878488942 56-57 17.63519201394367 24.79781448787838 39.403992407820546 18.163001090357398 58-59 17.95112270832123 24.427314228264553 39.373998791156964 18.247564272257257 60-61 17.996252710643382 23.837760278112476 39.95600522660224 18.209981784641904 62-63 17.754897665005732 23.645329670272865 40.673360312769006 17.926412351952397 64-65 17.63675321219124 23.50741693236331 40.954086503616914 17.901743351828536 66-67 17.72644456846723 23.468345619929078 40.94517423282615 17.86003557877755 68-69 17.990752330062538 23.62864242542797 40.59209529140541 17.788509953104086 70-71 18.109744585898884 24.029063928276692 40.137641854505006 17.723549631319415 72-73 18.119670084691386 24.073118668229657 40.01289074155676 17.794320505522197 74-75 18.202961994900672 24.34423161625582 39.73927455525617 17.713531833587336 76-77 18.22526033427504 24.673028670166328 39.36801396555511 17.73369703000353 78-79 18.10177599322206 24.69946110052374 39.315208165290215 17.88355474096398 80-81 18.10680781149349 24.951894195146714 39.09053403905747 17.85076395430233 82-83 18.049555222609953 25.085585411868195 38.956641170514594 17.908218195007255 84-85 18.17585345934494 24.96105518403138 38.852115571733265 18.010975784890416 86-87 18.193610542539673 25.183828908091595 38.662347763898744 17.96021278546999 88-89 18.275296693443792 25.194531891029882 38.541952717635844 17.988218697890474 90-91 18.215906105501762 24.986292973969213 38.561175312733695 18.236625607795332 92-93 18.188376907660583 24.943497018627685 38.53979960413794 18.328326469573792 94-95 18.329511671662846 24.98583031656758 38.33548144562408 18.349176566145488 96-97 18.398742025740543 24.974788199160926 38.119632864070915 18.506836911027623 98-99 18.423224922762042 25.04129938013192 38.08273275449754 18.45274294260849 100-101 18.65586413977253 25.197863784522383 37.53640641939906 18.60986565630603 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167018.0 1 89335.0 2 8137.0 3 3579.5 4 2437.0 5 1908.0 6 1382.0 7 1236.5 8 1216.0 9 1209.0 10 1291.0 11 1349.5 12 1438.5 13 2591.0 14 4833.0 15 12633.5 16 376150.0 17 384341.0 18 23546.5 19 11346.5 20 12348.5 21 15183.5 22 18717.0 23 22930.5 24 27592.0 25 32418.0 26 37797.5 27 43364.0 28 48654.5 29 53617.5 30 58300.5 31 62905.5 32 67488.5 33 70636.5 34 72048.0 35 74858.5 36 79560.0 37 86285.5 38 94272.5 39 102743.5 40 110989.5 41 119315.5 42 129575.0 43 137879.5 44 142790.0 45 146006.0 46 147193.0 47 145919.5 48 144169.0 49 143145.5 50 139405.0 51 133098.5 52 123754.5 53 113963.0 54 106714.0 55 99001.5 56 90272.0 57 81203.0 58 73432.5 59 66559.0 60 59191.0 61 51861.5 62 45606.0 63 39554.0 64 33814.0 65 28490.5 66 23175.0 67 18265.0 68 14000.5 69 10695.0 70 7784.0 71 5316.5 72 3527.0 73 2263.5 74 1408.0 75 743.5 76 358.5 77 175.0 78 76.0 79 45.0 80 24.5 81 9.0 82 6.5 83 4.0 84 2.5 85 3.0 86 3.5 87 3.0 88 3.5 89 3.0 90 1.0 91 1.5 92 3.0 93 1.5 94 4.0 95 4.0 96 1.5 97 2.5 98 1.0 99 1.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.002047134125953339 76-77 0.002708834045453408 78-79 0.0070409007059304245 80-81 0.006906492909781972 82-83 0.00145780763514859 84-85 4.0322338844535463E-4 86-87 0.001623232615023607 88-89 0.001261365471547007 90-91 1.1372967366407439E-4 92-93 2.0678122484377164E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4836029.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.58279551636146 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61196342618884 30.22176565225398 2 8.685155787304161 6.354545563893829 3 2.5255559886800745 2.7717569489701583 4 1.2664819632233433 1.8532580274303836 5 0.8177918688254515 1.4958556356092292 6 0.5695038114941056 1.2500424889006398 7 0.47253355376466927 1.2100618860394743 8 0.3805040740672145 1.1135922187794691 9 0.32136244095945415 1.0580702817832643 >10 2.22138927219237 14.646350310068096 >50 0.09417057282239982 2.2918315235330358 >100 0.02416956142878364 1.7610414733249777 >500 0.004254975477911684 1.0941160151500458 >1k 0.0036876454080733826 3.005171431148617 >5k 6.240630690585725E-4 1.6575597140023501 >10k+ 8.509950941707807E-4 28.214980829112413 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 802502 16.59423464995764 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 208216 4.305515951207075 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 166071 3.434036479103 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA 25468 0.5266304234321175 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA 24131 0.4989837736705052 No Hit ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 16898 0.3494189137410053 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG 16327 0.3376117058024259 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 15147 0.3132115212708608 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC 14195 0.2935259486657338 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 12758 0.2638114866556838 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGG 12745 0.2635426710633869 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCA 12387 0.2561399032139799 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11039 0.22826579410503947 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGA 10826 0.22386135401586715 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 10752 0.22233117295202323 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8801 0.1819881559850034 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCC 8621 0.1782660939378155 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 8581 0.17743896903844042 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 8508 0.17592946609708088 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA 8382 0.17332402266404937 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 7916 0.16368801758632961 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGT 6268 0.12961047173207604 No Hit ATATCTCGAGGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 6227 0.12876266871021658 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCT 6059 0.12528874413284122 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTCG 5346 0.11054524280148031 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT 5156 0.10661639952944865 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC 4993 0.10324586556449518 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAG 4865 0.10059906588649489 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010752623691876123 0.0 0.0 0.0 0.0 2 0.001137296736640744 0.0 0.0 0.0 0.0 3 0.0011993311040938753 0.0 0.0 0.0 0.0 4 0.001282043594031384 0.0 0.0 0.0 0.0 5 0.0013233998390001383 0.0 0.0 0.0 0.0 6 0.001406112328937647 0.0 0.0 0.0 0.0 7 0.0014474685739064013 0.0 0.0 0.0 0.0 8 0.0015508591863282871 0.0 0.0 0.0 0.0 9 0.0017162841662033044 0.0 0.0 0.0 0.0 10-11 0.0019127263298048874 0.0 0.0 1.033906124218858E-5 0.0 12-13 0.0021505247383752247 0.0 0.0 2.067812248437716E-5 0.0 14-15 0.0023986622081877506 0.0 0.0 2.067812248437716E-5 0.0 16-17 0.002719173106695597 0.0 0.0 2.067812248437716E-5 0.0 18-19 0.0030190058827190653 0.0 0.0 2.067812248437716E-5 0.0 20-21 0.0034118902099222314 0.0 0.0 2.067812248437716E-5 0.0 22-23 0.003866808904578529 0.0 0.0 2.067812248437716E-5 0.0 24-25 0.004394101027930147 0.0 0.0 2.067812248437716E-5 0.0 26-27 0.004849019722586444 0.0 0.0 2.067812248437716E-5 0.0 28-29 0.005562414948297456 0.0 0.0 2.067812248437716E-5 0.0 30-31 0.006224114867797526 0.0 0.0 2.067812248437716E-5 0.0 32-33 0.006668694501211634 0.0 0.0 2.067812248437716E-5 0.0 34-35 0.007040900705930424 0.0 0.0 4.135624496875432E-5 0.0 36-37 0.007402767849407024 0.0 0.0 4.135624496875432E-5 0.0 38-39 0.007826669360336755 0.0 0.0 4.135624496875432E-5 0.0 40-41 0.008105824013875848 0.0 0.0 4.135624496875432E-5 0.0 42-43 0.008415995851141505 0.0 0.0 4.135624496875432E-5 0.0 44-45 0.008798541117102482 0.0 0.0 4.135624496875432E-5 0.0 46-47 0.00910871295436814 0.0 0.0 4.135624496875432E-5 0.0 48-49 0.009367189485422853 0.0 0.0 5.1695306210942906E-5 0.0 50-51 0.009501597281571305 1.033906124218858E-5 0.0 6.203436745313149E-5 0.0 52-53 0.010700928385665182 2.067812248437716E-5 0.0 6.203436745313149E-5 0.0 54-55 0.012262126633235657 2.067812248437716E-5 0.0 6.203436745313149E-5 0.0 56-57 0.013616543655962361 2.067812248437716E-5 0.0 8.271248993750864E-5 0.0 58-59 0.015570626230736004 2.067812248437716E-5 0.0 8.271248993750864E-5 0.0 60-61 0.017535047866751835 2.067812248437716E-5 0.0 8.271248993750864E-5 0.0 62-63 0.019975066319908336 2.067812248437716E-5 0.0 8.271248993750864E-5 0.0 64-65 0.023355939346104006 2.067812248437716E-5 0.0 8.271248993750864E-5 0.0 66-67 0.02789478723142479 2.067812248437716E-5 0.0 1.2406873490626298E-4 0.0 68-69 0.03358127091462851 2.067812248437716E-5 0.0 1.2406873490626298E-4 0.0 70-71 0.03998114982354324 2.067812248437716E-5 0.0 1.2406873490626298E-4 0.0 72-73 0.047073745835684605 2.067812248437716E-5 0.0 1.2406873490626298E-4 0.0 74-75 0.057485180506568506 2.067812248437716E-5 0.0 1.2406873490626298E-4 0.0 76-77 0.07044002424303081 2.067812248437716E-5 0.0 1.2406873490626298E-4 0.0 78-79 0.08616573639239963 2.067812248437716E-5 0.0 1.3440779614845154E-4 0.0 80-81 0.10586164805876888 2.067812248437716E-5 0.0 1.4474685739064013E-4 0.0 82-83 0.12951742018089635 2.067812248437716E-5 0.0 1.4474685739064013E-4 0.0 84-85 0.15720542618747735 2.067812248437716E-5 0.0 1.4474685739064013E-4 0.0 86-87 0.18889464889478536 2.067812248437716E-5 0.0 1.654249798750173E-4 0.0 88-89 0.22592916626430487 2.067812248437716E-5 0.0 1.654249798750173E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 103800 0.0 92.92536 1 TATCTCG 104155 0.0 92.47639 2 ATCTCGA 105555 0.0 91.17785 3 ATATGGA 54155 0.0 90.89349 1 TCTCGAG 105895 0.0 90.79091 4 TATGGAT 54075 0.0 90.65026 2 CTCGAGG 106600 0.0 90.19492 5 TCGAGGG 106740 0.0 90.02322 6 AGGGCGC 107655 0.0 89.36839 9 CGAGGGC 108270 0.0 88.78178 7 GAGGGCG 108560 0.0 88.59712 8 ATGGATC 56115 0.0 87.0585 3 GGATCCG 57410 0.0 84.2508 5 TGGATCC 57890 0.0 83.80659 4 GATCCGG 58410 0.0 82.92225 6 ATCCGGC 59445 0.0 81.59834 7 TCCGGCG 60450 0.0 80.25746 8 CCGGCGC 61575 0.0 79.068825 9 GTCGACG 805 0.0 63.725574 1 TCGACGG 885 0.0 58.501778 2 >>END_MODULE