FastQCFastQC Report
Thu 26 May 2016
SRR1051299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4836029
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81392516.83044084309668No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT676141.3981305736586773No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT384490.7950531314018174No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT303860.6283254298102844No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT153570.31755392699258006No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT103110.21321212093641292No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50510.10444519666858905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA118600.073.768381
TATGGAT121400.071.796462
ATGGATC137650.063.1826443
ATATCTC57000.063.182121
GGATCCG144000.060.165575
TGGATCC149750.057.9822464
TATCTCG64750.055.1650122
GATCCGG161300.053.7420436
ATCCGGC172000.050.398797
TCCGGCG178400.048.644018
CCGGCGC190600.045.4307029
ATCTCGA88600.040.2616773
TCTCGAG97400.036.526544
CTCGAGG103700.034.353285
TCGAGGG106150.033.5603876
AGGGCGC111250.032.0645839
CGAGGGC120350.029.6400897
GAGGGCG124050.028.8326038
CCGTCGA185850.026.84844816-17
GTCGACT186500.026.76760918-19
Produced by FastQC (version 0.11.5)