##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051299_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4836029 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32297386967696 33.0 31.0 34.0 31.0 34.0 2 32.50780154544152 34.0 31.0 34.0 31.0 34.0 3 32.537169648899955 34.0 31.0 34.0 31.0 34.0 4 36.04230206229119 37.0 35.0 37.0 35.0 37.0 5 35.782149776190344 37.0 35.0 37.0 33.0 37.0 6 35.60295585489665 37.0 35.0 37.0 33.0 37.0 7 35.41653306876365 37.0 35.0 37.0 32.0 37.0 8 35.331007940605815 37.0 35.0 37.0 32.0 37.0 9 36.78066756837066 39.0 37.0 39.0 32.0 39.0 10-11 36.713585981390935 39.0 37.0 39.0 32.0 39.0 12-13 36.724780599950904 39.0 37.0 39.0 32.0 39.0 14-15 38.09280672220948 40.0 37.0 41.0 32.0 41.0 16-17 38.080866243771496 39.5 37.5 41.0 32.5 41.0 18-19 37.61432323916999 38.5 36.0 41.0 33.5 41.0 20-21 37.648932005990865 39.0 35.0 41.0 34.0 41.0 22-23 37.79191791447074 39.0 35.0 41.0 34.5 41.0 24-25 37.91570056755243 39.0 35.0 41.0 35.0 41.0 26-27 37.86735222224681 39.0 35.0 41.0 35.0 41.0 28-29 37.77244491296475 39.0 35.0 41.0 35.0 41.0 30-31 37.68142167881955 39.0 35.0 41.0 35.0 41.0 32-33 37.61463051607011 39.0 35.0 41.0 35.0 41.0 34-35 37.608954785010596 39.0 35.0 41.0 35.0 41.0 36-37 37.60552914384922 39.0 35.0 41.0 35.0 41.0 38-39 37.60357599178996 39.0 35.0 41.0 35.0 41.0 40-41 37.56347976821479 39.0 35.0 41.0 35.0 41.0 42-43 37.45722182393861 39.0 35.0 40.0 34.5 41.0 44-45 37.323027632795416 39.0 35.0 40.0 34.0 41.0 46-47 37.11470423357676 39.0 35.0 40.0 33.0 41.0 48-49 36.80470650610242 39.0 35.0 40.0 33.0 41.0 50-51 36.64757117875017 38.5 35.0 40.0 33.0 41.0 52-53 36.45754274839956 38.0 35.0 40.0 32.5 41.0 54-55 36.22824728718541 38.0 35.0 40.0 32.0 41.0 56-57 36.04322337603848 37.0 35.0 40.0 31.5 41.0 58-59 35.77379653844094 37.0 35.0 40.0 31.0 41.0 60-61 35.47968384804971 36.0 35.0 40.0 31.0 41.0 62-63 35.15875576842075 35.5 35.0 40.0 31.0 41.0 64-65 34.833150917829485 35.0 34.5 39.0 30.0 41.0 66-67 34.458934592823994 35.0 34.0 39.0 29.5 40.0 68-69 34.07212704886592 35.0 34.0 37.5 29.0 40.0 70-71 33.779968647830685 35.0 34.0 37.0 29.0 40.0 72-73 33.42917598302243 35.0 33.0 36.5 29.0 39.0 74-75 33.108136448313275 35.0 33.0 36.0 29.0 39.0 76-77 32.83594825423917 35.0 33.0 36.0 28.0 38.5 78-79 32.554923574693206 35.0 33.0 35.0 27.5 37.0 80-81 32.31198696285733 35.0 33.0 35.0 27.0 37.0 82-83 32.079862941268544 35.0 33.0 35.0 27.0 36.0 84-85 31.857371719648498 35.0 33.0 35.0 27.0 36.0 86-87 31.659828239243396 35.0 33.0 35.0 26.0 36.0 88-89 31.459322824573633 35.0 33.0 35.0 25.0 35.5 90-91 31.265818815395853 35.0 33.0 35.0 24.5 35.0 92-93 31.056974430881205 34.5 33.0 35.0 24.0 35.0 94-95 30.851678618966098 34.0 32.5 35.0 23.5 35.0 96-97 30.65843722194387 34.0 32.0 35.0 20.0 35.0 98-99 30.423402961396633 34.0 32.0 35.0 16.5 35.0 100-101 29.29242401151854 33.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13785308967666765 1101 2 0.14885748384349995 1101 3 0.1478600597813724 1101 4 0.13389711719645447 1101 5 0.18733359597738541 1101 6 0.2294996446741422 1101 7 0.2702776995310785 1101 8 0.22827355681609873 1101 9 0.24696779118183798 1101 10-11 0.17873078031807665 1101 12-13 0.16987107671885582 1101 14-15 0.23134998937528195 1101 16-17 0.2048562097699218 1101 18-19 0.2754798769936997 1101 20-21 0.20870795073085446 1101 22-23 0.2440478989015702 1101 24-25 0.179941359445408 1101 26-27 0.2340832628377072 1101 28-29 0.28460393599031164 1101 30-31 0.2933288064132782 1101 32-33 0.35223823093539863 1101 34-35 0.3693778304576725 1101 36-37 0.3536438533595643 1101 38-39 0.3842312580329974 1101 40-41 0.3274133727886408 1101 42-43 0.3550222734794275 1101 44-45 0.3025341257895704 1101 46-47 0.32219509704057003 1101 48-49 0.3238286997385984 1101 50-51 0.28247490155727917 1101 52-53 0.32344726602341467 1101 54-55 0.32861817059744425 1101 56-57 0.39124833138247794 1101 58-59 0.3800466451947173 1101 60-61 0.32058802737328307 1101 62-63 0.3356100516657534 1101 64-65 0.3957544977825904 1101 66-67 0.40926278765011403 1101 68-69 0.47251880978738825 1101 70-71 0.30896939411950797 1101 72-73 0.33635547498066387 1101 74-75 0.36575086759888364 1101 76-77 0.3687520189835709 1101 78-79 0.3476130140313529 1101 80-81 0.276502782157273 1101 82-83 0.2500467500719097 1101 84-85 0.2516256784677786 1101 86-87 0.3076581272379535 1101 88-89 0.3106523407312167 1101 90-91 0.31630506961992566 1101 92-93 0.34280053093257123 1101 94-95 0.35639421828740225 1101 96-97 0.3731676686402956 1101 98-99 0.4076964344444818 1101 100-101 0.4230099889647079 1102 1 0.1778161594484189 1102 2 0.16651491755702352 1102 3 0.16484689536699904 1102 4 0.13692789911996783 1102 5 0.1843648001190843 1102 6 0.2045566104621912 1102 7 0.1911006209877968 1102 8 0.19851248240153296 1102 9 0.15607498984341106 1102 10-11 0.17561062359828838 1102 12-13 0.10501266041726609 1102 14-15 0.09566698809317842 1102 16-17 0.21042572704304519 1102 18-19 0.2056177989593806 1102 20-21 0.156971113065822 1102 22-23 0.1397075474782028 1102 24-25 0.18007135194766732 1102 26-27 0.2054891899729867 1102 28-29 0.2080184888925487 1102 30-31 0.2410083491501993 1102 32-33 0.24260949776025598 1102 34-35 0.22829152565496003 1102 36-37 0.20443881969499245 1102 38-39 0.2642737650267293 1102 40-41 0.253177692658916 1102 42-43 0.32441335643738256 1102 44-45 0.3012125155460623 1102 46-47 0.3354443313245312 1102 48-49 0.31034017547828086 1102 50-51 0.27192303149908525 1102 52-53 0.27168624785179674 1102 54-55 0.2977271674490325 1102 56-57 0.31051740279161066 1102 58-59 0.3261017569458886 1102 60-61 0.3460082552681243 1102 62-63 0.3346381180847118 1102 64-65 0.3157729866613437 1102 66-67 0.31817052188714 1102 68-69 0.37416839166860427 1102 70-71 0.3237422775726344 1102 72-73 0.24720325423579936 1102 74-75 0.2833851704101136 1102 76-77 0.27911709908423177 1102 78-79 0.3475388683993472 1102 80-81 0.3142374825903076 1102 82-83 0.27875057589938024 1102 84-85 0.3376721596990713 1102 86-87 0.334972395796445 1102 88-89 0.3234537702526623 1102 90-91 0.3023488971346886 1102 92-93 0.3218878187986327 1102 94-95 0.3192896170994075 1102 96-97 0.35951804002198173 1102 98-99 0.3329923931510663 1102 100-101 0.5056786059149871 1103 1 0.121988824799665 1103 2 0.1525184602880998 1103 3 0.14063127315955626 1103 4 0.10264930440518327 1103 5 0.12044055888010519 1103 6 0.1673147212312429 1103 7 0.20564915337867973 1103 8 0.079185079520812 1103 9 0.15865694058934565 1103 10-11 0.03169343333190966 1103 12-13 0.08601992329464281 1103 14-15 0.17073166710633103 1103 16-17 0.12709189561706324 1103 18-19 0.15482726130888125 1103 20-21 0.19544074346182327 1103 22-23 0.1347163865361125 1103 24-25 0.1004647949268076 1103 26-27 0.13202372154491826 1103 28-29 0.17110994480106712 1103 30-31 0.1170964712438547 1103 32-33 0.17786193966283292 1103 34-35 0.23537334298610801 1103 36-37 0.23617077102854012 1103 38-39 0.22863965828609878 1103 40-41 0.17262191576624986 1103 42-43 0.197671053594334 1103 44-45 0.18953770378571022 1103 46-47 0.18991093810969772 1103 48-49 0.20481637714328826 1103 50-51 0.16487495859867352 1103 52-53 0.19853465856402153 1103 54-55 0.24135044334003197 1103 56-57 0.19871831354998193 1103 58-59 0.25351441912603434 1103 60-61 0.24729139779764608 1103 62-63 0.26715554671668684 1103 64-65 0.298344294109711 1103 66-67 0.3241414950130306 1103 68-69 0.31194589069399115 1103 70-71 0.2134156780884311 1103 72-73 -0.17331204307119208 1103 74-75 -0.5418692718056093 1103 76-77 -0.3657587108699545 1103 78-79 -0.2315520112832843 1103 80-81 -0.17022240034796 1103 82-83 -0.14771826802179433 1103 84-85 -0.1639237796288846 1103 86-87 -0.16032421292360866 1103 88-89 -0.15896335396258365 1103 90-91 -0.16238044894545212 1103 92-93 -0.06003048111046283 1103 94-95 -0.016191330190423514 1103 96-97 -0.03819103197769991 1103 98-99 -0.032780940312040485 1103 100-101 0.015750109941681245 1104 1 0.14806425416450963 1104 2 0.16063840116736827 1104 3 0.15669244330803167 1104 4 0.09765298363457475 1104 5 0.1003106290275042 1104 6 0.10769766567276662 1104 7 0.13711098405531885 1104 8 0.09485216501533955 1104 9 0.12914156002563004 1104 10-11 0.1614229360406938 1104 12-13 0.1474128327425248 1104 14-15 0.24770970447572438 1104 16-17 0.26028373999568544 1104 18-19 0.2856154530793873 1104 20-21 0.22995403160356886 1104 22-23 0.2750095753795705 1104 24-25 0.21010740735835043 1104 26-27 0.17975898980511573 1104 28-29 0.12677079476065956 1104 30-31 0.19000823215970541 1104 32-33 0.22519819798370122 1104 34-35 0.24559504120926334 1104 36-37 0.22439977173944925 1104 38-39 0.2418498919080463 1104 40-41 0.1919594310858983 1104 42-43 0.16754807004274141 1104 44-45 0.17579541569554635 1104 46-47 0.1664795729815367 1104 48-49 0.1529327306013002 1104 50-51 0.21827123446881558 1104 52-53 0.22654721915456122 1104 54-55 0.23522160574673734 1104 56-57 0.20463730101352695 1104 58-59 0.16314935305827305 1104 60-61 0.13619916465738413 1104 62-63 0.08986167696798475 1104 64-65 -0.217522059363759 1104 66-67 -0.46761478076395946 1104 68-69 -0.20268928569006306 1104 70-71 -0.155636336572762 1104 72-73 -0.05742451911374502 1104 74-75 0.019829427797468213 1104 76-77 -0.09667346186678571 1104 78-79 -0.058686632542347184 1104 80-81 -0.05102688780637976 1104 82-83 -0.04361900246015438 1104 84-85 -0.02293929916189441 1104 86-87 -0.07188857073120758 1104 88-89 -0.12747133685069656 1104 90-91 -0.15881037953787924 1104 92-93 -0.1545919521444148 1104 94-95 -0.14045426071832523 1104 96-97 -0.09707570378275321 1104 98-99 -0.08774585775158528 1104 100-101 -0.08426493999937179 1105 1 0.11414410362312566 1105 2 0.1289844367099917 1105 3 0.1397157704544867 1105 4 0.1072686034485244 1105 5 0.1663750788941627 1105 6 0.14683134036138057 1105 7 0.15599294322799295 1105 8 0.21300585991474463 1105 9 0.24839331975505274 1105 10-11 0.18761024568308926 1105 12-13 0.19857841277335808 1105 14-15 0.28276854736436263 1105 16-17 0.30159141757274455 1105 18-19 0.23800619315468907 1105 20-21 0.20994359706281784 1105 22-23 0.22289367605110755 1105 24-25 0.14407488054333584 1105 26-27 0.19152317022599163 1105 28-29 0.19556901777556135 1105 30-31 0.20544774046097558 1105 32-33 0.2700070440125657 1105 34-35 0.27952144213790575 1105 36-37 0.24479315268350632 1105 38-39 0.23610810987014474 1105 40-41 0.20155029976691452 1105 42-43 0.20653533141292257 1105 44-45 0.22909593369438852 1105 46-47 0.2543268428454297 1105 48-49 0.2785916381133404 1105 50-51 0.2286564332152068 1105 52-53 0.1729737074784481 1105 54-55 0.23203093329582458 1105 56-57 0.20684355750292838 1105 58-59 0.2332608785981165 1105 60-61 0.18620090054218252 1105 62-63 0.20494415793980636 1105 64-65 0.31391648709907116 1105 66-67 0.34853241160916326 1105 68-69 0.32564048442169735 1105 70-71 0.2998767524092969 1105 72-73 0.29705046868948415 1105 74-75 0.37191206985700376 1105 76-77 0.293879121849983 1105 78-79 0.2388923721023133 1105 80-81 0.19866773970729668 1105 82-83 0.21645135806920024 1105 84-85 0.23905365645132193 1105 86-87 0.18436393505806592 1105 88-89 0.17960200605494236 1105 90-91 0.1722989778588051 1105 92-93 0.06001946198026076 1105 94-95 0.036009847925267735 1105 96-97 -0.0030922865009088696 1105 98-99 0.08722435825158215 1105 100-101 0.02636478110894913 1106 1 0.050780499830935355 1106 2 0.04594751484891901 1106 3 0.09391126400416994 1106 4 0.05795427326124525 1106 5 0.06843452123314364 1106 6 0.04954383465719303 1106 7 2.663492441641324E-4 1106 8 0.007797923520136862 1106 9 0.07380283698897472 1106 10-11 0.07362394956391682 1106 12-13 0.04662875013786305 1106 14-15 0.11655413389487279 1106 16-17 0.08716476544056206 1106 18-19 0.057956583981237486 1106 20-21 0.10296340871171594 1106 22-23 0.0924894201944042 1106 24-25 0.0910530449834539 1106 26-27 -0.004192264646874833 1106 28-29 -0.05110642470501858 1106 30-31 0.047188478390189914 1106 32-33 0.09101382141221848 1106 34-35 0.105280984412083 1106 36-37 -0.05772574987498302 1106 38-39 -0.023407413011774736 1106 40-41 -0.01514575375527727 1106 42-43 -0.03303580906786863 1106 44-45 0.0037338797401176294 1106 46-47 0.044845744400269894 1106 48-49 -0.0047762528341763755 1106 50-51 -0.01834106704434646 1106 52-53 -0.08411256248970034 1106 54-55 -0.07102821607211496 1106 56-57 -0.05725524749208688 1106 58-59 -0.028238080909751773 1106 60-61 -0.05969706558554577 1106 62-63 -0.07078268734404958 1106 64-65 0.0016184427170529148 1106 66-67 0.012853949108283302 1106 68-69 0.04544155053214638 1106 70-71 0.04440440958911296 1106 72-73 0.018196038207250353 1106 74-75 0.014435858290248405 1106 76-77 0.06305933116592399 1106 78-79 0.0556204866443295 1106 80-81 -0.022058726213877833 1106 82-83 -0.05132675342421322 1106 84-85 -0.05061262965600122 1106 86-87 -0.014548897010456585 1106 88-89 -0.04538012549004833 1106 90-91 -0.06822713168341821 1106 92-93 0.030032207832803692 1106 94-95 0.008476233602987548 1106 96-97 -0.03343828012510741 1106 98-99 -0.1158665684103255 1106 100-101 -0.08070582313965602 1107 1 0.07170831087366025 1107 2 0.06647029765883872 1107 3 0.08938474856344669 1107 4 0.06311425105494095 1107 5 0.03670995801197563 1107 6 0.046128273117119534 1107 7 0.023438306700121814 1107 8 4.438639283677048E-5 1107 9 0.02628701860233207 1107 10-11 0.11062759111783294 1107 12-13 0.07108847160713339 1107 14-15 0.056348229141455874 1107 16-17 0.07389060559756899 1107 18-19 0.02151814877036884 1107 20-21 0.012398774674842628 1107 22-23 -0.03162584132913793 1107 24-25 -0.03191858089554955 1107 26-27 0.027981691335291714 1107 28-29 0.03616358902676353 1107 30-31 0.08434852274945825 1107 32-33 0.10888135340130134 1107 34-35 0.09646164678199654 1107 36-37 0.02777614394754835 1107 38-39 0.03671816790387794 1107 40-41 -0.008830185184876882 1107 42-43 -0.022718839915690126 1107 44-45 0.01080413363763455 1107 46-47 0.053938209374408075 1107 48-49 0.028170466738593802 1107 50-51 0.02848423680713097 1107 52-53 0.10935423294304059 1107 54-55 0.10931690614849998 1107 56-57 0.03565928845166155 1107 58-59 0.020918501889354957 1107 60-61 0.030235740185553084 1107 62-63 0.05102514914835865 1107 64-65 0.14717032683554976 1107 66-67 0.09425511989983448 1107 68-69 0.15399857396757 1107 70-71 0.13308854735257825 1107 72-73 0.1323298631491383 1107 74-75 0.09750145019334866 1107 76-77 -0.020191823546767296 1107 78-79 0.04950960158194562 1107 80-81 0.09371091392237219 1107 82-83 0.006167984674149807 1107 84-85 0.04623206442437677 1107 86-87 0.06399496901783053 1107 88-89 0.03154313925914565 1107 90-91 0.1105912647500027 1107 92-93 0.009742996187274144 1107 94-95 0.0938519082767364 1107 96-97 0.09867560343093018 1107 98-99 0.002486056449292562 1107 100-101 -0.14114967540592716 1108 1 0.040367987127936544 1108 2 0.045447418075497126 1108 3 0.05327573133067176 1108 4 0.08052607454420979 1108 5 0.03844840656174853 1108 6 0.07524508295193044 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-0.0398318678665035 2214 28-29 -0.03416602400976387 2214 30-31 -0.008758292689989844 2214 32-33 -0.056618580450390255 2214 34-35 -0.01859826811644183 2214 36-37 -0.024739976448337586 2214 38-39 -0.08468927162139295 2214 40-41 -0.125027099440004 2214 42-43 -0.09462929726481661 2214 44-45 -0.515938774031035 2214 46-47 -0.3628734384437422 2214 48-49 -0.10303496877686769 2214 50-51 -0.10608773561107654 2214 52-53 -0.14158886702502826 2214 54-55 -0.01972337078230879 2214 56-57 0.009220363056080316 2214 58-59 -0.014776712789831947 2214 60-61 0.02191510697360144 2214 62-63 -0.052347738868249394 2214 64-65 -0.061253961475294716 2214 66-67 -0.009722859580158172 2214 68-69 0.021815077026076324 2214 70-71 0.02107170741374631 2214 72-73 0.054359240008146514 2214 74-75 0.08211330285472229 2214 76-77 0.05407148642225934 2214 78-79 0.016590686433076485 2214 80-81 -0.014289103467788777 2214 82-83 0.05606228640052535 2214 84-85 0.08933085124030171 2214 86-87 0.091679287349276 2214 88-89 0.0942627141925243 2214 90-91 0.019833350388395843 2214 92-93 0.05922207425493298 2214 94-95 0.0703099097626847 2214 96-97 0.12029104404193802 2214 98-99 0.1022415731095414 2214 100-101 0.018093223289628924 2215 1 -0.1960569132244956 2215 2 -0.21181276940588845 2215 3 -0.23621408781142605 2215 4 -0.13279533757680184 2215 5 -0.17582113775193164 2215 6 -0.17750267455886615 2215 7 -0.23182691535239286 2215 8 -0.17738812019126016 2215 9 -0.21849667467352418 2215 10-11 -0.2720829023083837 2215 12-13 -0.24814946554395334 2215 14-15 -0.2914727394475065 2215 16-17 -0.2912620015534273 2215 18-19 -0.27748507663012845 2215 20-21 -0.2934132058657326 2215 22-23 -0.23677677760190363 2215 24-25 -0.17478397177058724 2215 26-27 -0.1253765611584683 2215 28-29 -0.160214860146219 2215 30-31 -0.24023169978010372 2215 32-33 -0.2099111907832807 2215 34-35 -0.19355906163436742 2215 36-37 -0.23354106942032615 2215 38-39 -0.2502232641320461 2215 40-41 -0.294364004594847 2215 42-43 -0.1846981121884923 2215 44-45 -0.18151940828381186 2215 46-47 -0.2530062452982378 2215 48-49 -0.23837441892571576 2215 50-51 -0.19227676067281152 2215 52-53 -0.18662561930943156 2215 54-55 -0.18601199911204702 2215 56-57 -0.17928476985789388 2215 58-59 -0.1921550483373835 2215 60-61 -0.2179321933864813 2215 62-63 -0.28421574288786644 2215 64-65 -0.33377118768055425 2215 66-67 -0.25584607378801394 2215 68-69 -0.13321234241503532 2215 70-71 -0.2147652496568213 2215 72-73 -0.16060314019802036 2215 74-75 -0.21147781013291933 2215 76-77 -0.26093891395207436 2215 78-79 -0.20321076328156096 2215 80-81 -0.13505291930467678 2215 82-83 -0.1325888622853455 2215 84-85 -0.16044442785726432 2215 86-87 -0.17592757325693853 2215 88-89 -0.14897654805587734 2215 90-91 -0.17637059913184316 2215 92-93 -0.18108281860315856 2215 94-95 -0.17593516030406064 2215 96-97 -0.16514955560935007 2215 98-99 -0.07389645618171414 2215 100-101 -0.22519222372270065 2216 1 -0.3933080461426961 2216 2 -0.35020497101642434 2216 3 -0.39298701514164947 2216 4 -0.2651119678379459 2216 5 -0.38211788081586207 2216 6 -0.425700112044602 2216 7 -0.43302758043243017 2216 8 -0.388846145164365 2216 9 -0.561164036848723 2216 10-11 -0.6119852268283168 2216 12-13 -0.6452049866863305 2216 14-15 -0.9358088656823043 2216 16-17 -0.8358341007938037 2216 18-19 -0.5152576453387638 2216 20-21 -0.4298738715978061 2216 22-23 -0.3835213412246361 2216 24-25 -0.2674054303823965 2216 26-27 -0.19082584880558784 2216 28-29 -0.3289491867526806 2216 30-31 -0.30690147450054184 2216 32-33 -0.22978632526637455 2216 34-35 -0.3729307566104083 2216 36-37 -0.33287129469989196 2216 38-39 -0.2419367580843783 2216 40-41 -0.30090213259015997 2216 42-43 -0.22374946027964882 2216 44-45 -0.13730097513414563 2216 46-47 -0.1262315574620274 2216 48-49 -0.2695446989765742 2216 50-51 -0.2952511029282192 2216 52-53 -0.29916831157949986 2216 54-55 -0.3183802708820096 2216 56-57 -0.16843737964500605 2216 58-59 -0.12395989298903487 2216 60-61 -0.19843550248568675 2216 62-63 -0.2672045604146831 2216 64-65 -0.2650914367595476 2216 66-67 -0.17482152451014343 2216 68-69 -0.21735696472130428 2216 70-71 -0.30933356706953674 2216 72-73 -0.24480109931515415 2216 74-75 -0.07591226388703376 2216 76-77 -0.058131624153233474 2216 78-79 -0.04596960656392923 2216 80-81 -0.16396401375255465 2216 82-83 -0.18963949313332762 2216 84-85 -0.22262452109887576 2216 86-87 -0.213556758533894 2216 88-89 -0.12228968990701716 2216 90-91 -0.17729199579992283 2216 92-93 -0.13102035324273942 2216 94-95 -0.1016041851793581 2216 96-97 -0.1086042551822608 2216 98-99 -0.14723685327361835 2216 100-101 -0.34143308702267205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 21.0 13 203.0 14 941.0 15 2577.0 16 4926.0 17 8326.0 18 16280.0 19 34087.0 20 35542.0 21 32393.0 22 31565.0 23 30578.0 24 30394.0 25 33591.0 26 39280.0 27 47630.0 28 57254.0 29 67001.0 30 79603.0 31 102786.0 32 146402.0 33 228860.0 34 423399.0 35 1002118.0 36 623804.0 37 840309.0 38 783721.0 39 132372.0 40 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.925331760347163 42.97309298899959 20.959743870573284 18.141831380079964 2 17.78914063583986 39.852655970425324 27.473160313968343 14.885043079766477 3 17.316149262132218 41.687487812831556 24.234676839200095 16.76168608583613 4 18.714982891955362 38.69575637366939 24.90952804460023 17.679732689775022 5 21.363230038529547 38.08211241082301 21.246977633922377 19.307679916725064 6 21.12855402645435 37.66273940871736 21.617674335699807 19.591032229128487 7 19.36735697821498 39.74444735546457 21.13171777919446 19.756477887125985 8 19.673269122248854 37.284681295335496 23.18178819854058 19.860261383875073 9 19.0412009522689 37.25773770173835 21.838454649465504 21.862606696527255 10-11 20.975608293498656 37.25278529140334 21.29130118946764 20.480305225630367 12-13 20.01518187752803 37.370940083279066 21.61550313283895 20.998374906353952 14-15 19.834930683831715 37.499992245704064 21.99019071225586 20.674886358208354 16-17 19.912039402575957 37.501977345462564 21.93841269355498 20.647570558406496 18-19 19.809786086890714 37.69235048011499 22.52632273296955 19.97154070002475 20-21 18.265440509145 37.895947274096166 24.22883526959826 19.609776947160572 22-23 18.68427588006606 38.259716804841325 23.982041877747218 19.073965437345393 24-25 17.710739534440343 37.0298130966543 26.012075196405977 19.24737217249938 26-27 17.722464029888986 37.06658913749277 27.38155623136255 17.8293906012557 28-29 17.8390472844559 37.172947060491154 27.190542074913115 17.797463580139823 30-31 17.82378683006243 37.017395884102434 27.2940050607637 17.86481222507144 32-33 17.778119196555686 37.10457484849657 27.345317821708683 17.77198813323907 34-35 17.889677667358903 37.15150384747486 27.16255423613051 17.79626424903573 36-37 17.795199325727783 37.05202140020252 27.24116211875487 17.911617155314826 38-39 17.728615771328087 37.147037373018236 27.291389278269424 17.832957577384253 40-41 17.880041662281183 37.22026894379666 27.006341359822283 17.89334803409988 42-43 17.898476208476 37.22594508841862 26.867198273624908 18.008380429480468 44-45 17.897587049209175 37.38476340815988 26.710737259846873 18.006912282784075 46-47 18.175097792010757 37.545649540149576 26.09271987409505 18.186532793744618 48-49 18.302154515616014 37.758044875247855 25.440593925305244 18.499206683830888 50-51 18.355214577910928 38.146131878034645 25.063611074292563 18.435042469761864 52-53 18.488991691323605 38.24166480391247 24.843740597916184 18.425602906847747 54-55 18.400282545865625 38.21110253888056 24.841683124728988 18.54693179052483 56-57 18.33710054261461 38.32735494348773 24.895983874372963 18.439560639524704 58-59 18.526574178938958 38.29737166588538 24.745953756687562 18.430100398488097 60-61 18.43884724429899 38.134366026341034 24.89714184923209 18.52964488012789 62-63 18.342869738787755 38.159645031078185 25.10173119309251 18.39575403704155 64-65 18.388744153519344 38.20132178694545 24.955195678106975 18.454738381428236 66-67 18.42571663652141 38.07181470582579 24.960913178973907 18.54155547867889 68-69 18.457137043636422 38.17606346033078 24.894712169840172 18.472087326192625 70-71 18.523989413628414 38.30044236707431 24.649976251176327 18.525591968120953 72-73 18.472907931881288 38.2202501722488 24.661209571160462 18.645632324709453 74-75 18.446860016761686 38.36918678527362 24.60520811599765 18.578745081967043 76-77 18.545484321950923 38.44427938707564 24.367192587141226 18.643043703832216 78-79 18.49723192313363 38.35396768712512 24.419063657393288 18.729736732347966 80-81 18.454486953349846 38.4344329991559 24.429916000028122 18.681164047466126 82-83 18.5424674337488 38.54224824379217 24.150700952938674 18.764583369520356 84-85 18.504169433227137 38.39468291029686 24.224244726406727 18.876902930069278 86-87 18.4627925101359 38.519899281001 24.226260843348953 18.791047365514142 88-89 18.595618429914296 38.576505641301985 23.965819890658224 18.862056038125495 90-91 18.505472154943654 38.41900038233849 24.065333355114287 19.010194107603574 92-93 18.476388375669377 38.457378977669485 24.139650527323138 18.926582119337994 94-95 18.577587107107917 38.43873765024982 23.975455895735944 19.008219346906316 96-97 18.513608996141258 38.403636537332595 23.962366644203335 19.12038782232282 98-99 18.493431257055146 38.43942391339636 24.04761860451459 19.019526225033907 100-101 18.540252756962374 38.33260718659876 23.77930322584914 19.347836830589728 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 782169.0 1 421291.5 2 39814.0 3 14941.5 4 9346.5 5 7436.0 6 6470.0 7 5877.0 8 5488.5 9 5108.5 10 4871.5 11 4646.0 12 4336.0 13 4873.0 14 6094.5 15 7948.5 16 35123.0 17 33883.0 18 5962.0 19 5486.5 20 6116.0 21 7112.0 22 8680.5 23 10671.5 24 12973.5 25 15730.0 26 18974.5 27 22971.0 28 27667.0 29 32272.0 30 36885.0 31 42326.0 32 48948.5 33 54699.0 34 59326.5 35 65398.0 36 72740.0 37 82174.0 38 91547.5 39 100486.0 40 110161.0 41 119563.0 42 130688.5 43 140561.5 44 146898.0 45 151179.5 46 153904.0 47 154334.0 48 154340.0 49 155406.0 50 153656.0 51 148384.5 52 138952.5 53 128895.5 54 121155.5 55 113306.0 56 104330.5 57 93962.0 58 85302.0 59 78315.0 60 70500.0 61 62492.5 62 54772.0 63 47495.5 64 40790.5 65 33992.5 66 27753.5 67 22099.0 68 17008.5 69 12687.5 70 9081.5 71 6217.0 72 4137.5 73 2731.0 74 1630.5 75 830.5 76 400.0 77 197.5 78 92.5 79 27.0 80 7.5 81 3.5 82 0.5 83 1.5 84 1.0 85 1.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16221987088993883 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.0678122484377164E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015508591863282874 82-83 8.478030218594636E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 4.135624496875432E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4836029.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.9630737589421 #Duplication Level Percentage of deduplicated Percentage of total 1 80.40591656119585 32.13267574190414 2 9.850747760407609 7.87332318659806 3 2.872150088865563 3.443398375442598 4 1.3834630557240786 2.2114974455469114 5 0.862756047532281 1.7239191781752954 6 0.6131952362232392 1.470309987229274 7 0.46950410349679006 1.3133978982717747 8 0.39174722751268076 1.2524338678360267 9 0.3214359704548181 1.1561012456456725 >10 2.66903708482429 20.1944827453632 >50 0.13617807250015374 3.62725919289056 >100 0.02033863728384788 1.2115168981885793 >500 0.0013497647734979404 0.39422388870483893 >1k 0.001816991004389646 1.641271739784696 >5k 5.1914028696846966E-5 0.10479027641734394 >10k+ 3.1148417218108184E-4 20.249398332001036 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 813925 16.83044084309668 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67614 1.3981305736586773 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 38449 0.7950531314018174 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 30386 0.6283254298102844 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15357 0.31755392699258006 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 10311 0.21321212093641292 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5051 0.10444519666858905 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001033906124218858 0.0 0.0 0.0 0.0 2 0.001033906124218858 0.0 0.0 0.0 0.0 3 0.0010545842467032352 0.0 0.0 0.0 0.0 4 0.0010752623691876123 0.0 0.0 0.0 0.0 5 0.0010752623691876123 0.0 0.0 0.0 0.0 6 0.0011166186141563668 0.0 0.0 0.0 0.0 7 0.001137296736640744 0.0 0.0 0.0 0.0 8 0.001157974859125121 0.0 0.0 0.0 0.0 9 0.0012200092265782525 0.0 0.0 0.0 0.0 10-11 0.0012923826552735727 0.0 0.0 0.0 0.0 12-13 0.0013647560839688926 0.0 0.0 0.0 0.0 14-15 0.0014371295126642128 0.0 0.0 0.0 0.0 16-17 0.0015611982475704756 0.0 0.0 0.0 0.0 18-19 0.0017473013499298702 0.0 0.0 0.0 0.0 20-21 0.002026456003468962 0.0 0.0 0.0 0.0 22-23 0.0023056106570080535 0.0 0.0 2.067812248437716E-5 0.0 24-25 0.002636460616758088 0.0 0.0 2.067812248437716E-5 0.0 26-27 0.0029673105765081224 0.0 0.0 2.067812248437716E-5 0.0 28-29 0.003577315189797249 0.0 0.0 2.067812248437716E-5 0.0 30-31 0.004052912006937924 0.0 0.0 2.067812248437716E-5 0.0 32-33 0.004342405721719204 0.0 0.0 2.067812248437716E-5 0.0 34-35 0.004621560375258295 0.0 0.0 2.067812248437716E-5 0.0 36-37 0.004911054090039576 0.0 0.0 2.067812248437716E-5 0.0 38-39 0.005272921233516177 0.0 0.0 2.067812248437716E-5 0.0 40-41 0.005490041519602137 0.0 0.0 2.067812248437716E-5 0.0 42-43 0.005727839928172474 0.0 0.0 2.067812248437716E-5 0.0 44-45 0.006069028949164697 0.0 0.0 2.067812248437716E-5 0.0 46-47 0.006317166418977223 0.0 0.0 2.067812248437716E-5 0.0 48-49 0.006565303888789749 0.0 0.0 2.067812248437716E-5 0.0 50-51 0.006720389807422577 0.0 0.0 3.1017183726565745E-5 0.0 52-53 0.007961077156485207 0.0 0.0 4.135624496875432E-5 0.0 54-55 0.009501597281571305 0.0 0.0 4.135624496875432E-5 0.0 56-57 0.01091804867175114 0.0 0.0 4.135624496875432E-5 0.0 58-59 0.012903148430251348 0.0 0.0 4.135624496875432E-5 0.0 60-61 0.014836552882540613 0.0 0.0 5.1695306210942906E-5 0.0 62-63 0.017224876029486177 0.0 0.0 6.203436745313149E-5 0.0 64-65 0.020564392810713088 0.0 0.0 6.203436745313149E-5 0.0 66-67 0.025103240696033875 0.0 0.0 8.271248993750864E-5 0.0 68-69 0.030841419685448537 0.0 0.0 8.271248993750864E-5 0.0 70-71 0.03724129859436327 0.0 0.0 8.271248993750864E-5 0.0 72-73 0.04434423366774683 0.0 0.0 8.271248993750864E-5 0.0 74-75 0.05475566833863073 0.0 0.0 8.271248993750864E-5 0.0 76-77 0.06737966211534298 0.0 0.0 1.0339061242188581E-4 0.0 78-79 0.08260909932508675 0.0 0.0 1.0339061242188581E-4 0.0 80-81 0.10165365013319813 0.0 0.0 1.1372967366407439E-4 0.0 82-83 0.12454433172340365 0.0 0.0 1.2406873490626298E-4 0.0 84-85 0.15206691275010964 0.0 0.0 1.4474685739064013E-4 0.0 86-87 0.18354935423257387 0.0 0.0 1.4474685739064013E-4 0.0 88-89 0.21976708576396048 0.0 0.0 1.4474685739064013E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATGGA 11860 0.0 73.76838 1 TATGGAT 12140 0.0 71.79646 2 ATGGATC 13765 0.0 63.182644 3 ATATCTC 5700 0.0 63.18212 1 GGATCCG 14400 0.0 60.16557 5 TGGATCC 14975 0.0 57.982246 4 TATCTCG 6475 0.0 55.165012 2 GATCCGG 16130 0.0 53.742043 6 ATCCGGC 17200 0.0 50.39879 7 TCCGGCG 17840 0.0 48.64401 8 CCGGCGC 19060 0.0 45.430702 9 ATCTCGA 8860 0.0 40.261677 3 TCTCGAG 9740 0.0 36.52654 4 CTCGAGG 10370 0.0 34.35328 5 TCGAGGG 10615 0.0 33.560387 6 AGGGCGC 11125 0.0 32.064583 9 CGAGGGC 12035 0.0 29.640089 7 GAGGGCG 12405 0.0 28.832603 8 CCGTCGA 18585 0.0 26.848448 16-17 GTCGACT 18650 0.0 26.767609 18-19 >>END_MODULE