FastQCFastQC Report
Thu 26 May 2016
SRR1051298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10613950
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT9653799.095379194362136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1721541.6219597793469913No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT486610.45846268354382674No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370690.34924792372302493No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA351450.3311208362579436No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA326410.3075292421765695No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC314350.2961668370399333No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC301150.2837303737063016No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT269870.25425972423084714No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC247620.2332967462631725No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT234730.22115235138661857No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT221730.20890431931561765No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG191080.18002722831745016No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA152030.14323602428878973No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC128910.12145337032867123No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG123190.11606423621743082No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT118280.11143824871984512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2574300.094.542991
TCGACGG2605150.093.423412
ATATCTC1761650.092.2485051
CGACGGC2646850.091.87623
GACGGCG2667050.091.208834
ACGGCGC2667500.091.1916665
TATCTCG1785100.090.895652
CGGCGCG2688350.090.4508366
ATCTCGA1845450.087.861413
TCTCGAG1857300.086.500364
CTCGAGG1898700.084.719345
TCGAGGG1928500.083.486576
AGGGCGC1972050.081.989729
CGAGGGC1968550.081.858027
GAGGGCG1991850.081.134178
CTCGACG21500.063.6270645
TCTCGAC25050.054.7996674
GGCGCGC4670250.052.8872577
CGCGCCG4673400.052.834339
GCGCGCC4674550.052.824388
Produced by FastQC (version 0.11.5)