##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051298_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10613950 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26724508783252 34.0 31.0 34.0 30.0 34.0 2 32.46196043885641 34.0 31.0 34.0 31.0 34.0 3 32.46326768074091 34.0 31.0 34.0 31.0 34.0 4 36.089391885207675 37.0 37.0 37.0 35.0 37.0 5 35.97479072352894 37.0 35.0 37.0 35.0 37.0 6 35.996271699037585 37.0 36.0 37.0 35.0 37.0 7 35.936222235831146 37.0 36.0 37.0 35.0 37.0 8 36.022288026606496 37.0 36.0 37.0 35.0 37.0 9 37.62393604642946 39.0 38.0 39.0 35.0 39.0 10-11 37.67659316277164 39.0 38.0 39.0 35.0 39.0 12-13 37.67690704214736 39.0 38.0 39.0 35.0 39.0 14-15 39.12066629294466 41.0 39.0 41.0 36.0 41.0 16-17 38.94341239595062 40.5 38.5 41.0 35.0 41.0 18-19 38.82569034148456 40.5 38.5 41.0 35.0 41.0 20-21 38.905266795113974 40.5 39.0 41.0 35.0 41.0 22-23 38.88631159935745 40.5 38.5 41.0 35.0 41.0 24-25 38.88419655265005 41.0 38.5 41.0 35.0 41.0 26-27 38.95123139830129 41.0 39.0 41.0 35.0 41.0 28-29 38.615272966237825 40.0 38.5 41.0 35.0 41.0 30-31 37.494723076705654 39.0 36.0 40.5 34.5 41.0 32-33 36.71989372476788 36.0 35.0 40.0 34.0 41.0 34-35 36.46397905586516 35.0 35.0 40.0 33.0 41.0 36-37 36.362035999792724 35.0 35.0 40.0 33.0 41.0 38-39 35.84298413879847 35.0 35.0 40.0 32.0 41.0 40-41 33.71192595593534 35.0 32.5 40.0 21.0 41.0 42-43 33.80323611850442 35.0 33.0 40.0 22.5 41.0 44-45 33.76924142284446 35.0 32.0 40.0 22.0 41.0 46-47 33.91157462584617 35.0 32.5 40.0 23.0 41.0 48-49 33.270769694600034 35.0 31.0 40.0 19.0 41.0 50-51 33.36647251965574 35.0 31.0 40.0 21.0 41.0 52-53 33.33934435342168 35.0 31.0 40.0 21.0 41.0 54-55 32.934907503803956 35.0 31.0 40.0 19.0 41.0 56-57 32.53350957937431 35.0 31.0 40.0 18.0 41.0 58-59 32.147606216347356 35.0 31.0 39.0 18.0 41.0 60-61 31.80997296011381 35.0 31.0 39.0 16.0 41.0 62-63 31.454691514469168 35.0 30.5 39.0 11.5 41.0 64-65 31.080011776953913 35.0 30.0 38.0 8.5 40.5 66-67 30.68215885697596 35.0 29.5 37.0 7.0 40.0 68-69 30.348271143165363 35.0 29.0 37.0 7.0 40.0 70-71 29.99063430673783 35.0 29.0 36.0 2.0 39.0 72-73 29.64329500327399 35.0 29.0 36.0 2.0 39.0 74-75 29.327803974957483 34.0 29.0 35.0 2.0 38.5 76-77 29.022868394895397 34.0 29.0 35.0 2.0 37.0 78-79 28.7898429425426 34.0 29.0 35.0 2.0 37.0 80-81 28.561210341107692 34.0 29.0 35.0 2.0 36.5 82-83 28.38728004183174 34.0 29.0 35.0 2.0 36.0 84-85 28.198793427517558 34.0 28.0 35.0 2.0 36.0 86-87 28.002602848138537 34.0 27.5 35.0 2.0 35.0 88-89 27.79750220229038 34.0 27.0 35.0 2.0 35.0 90-91 27.585075867137114 34.0 27.0 35.0 2.0 35.0 92-93 27.308410252545002 34.0 27.0 35.0 2.0 35.0 94-95 27.03463291234649 34.0 25.5 35.0 2.0 35.0 96-97 26.70672817377131 34.0 25.0 35.0 2.0 35.0 98-99 26.30882367073521 34.0 25.0 35.0 2.0 35.0 100-101 24.709267002388366 32.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2250729571149961 1101 2 0.19922990691837583 1101 3 0.22170138773767434 1101 4 0.1624053792768123 1101 5 0.17007006967121185 1101 6 0.20465164803970026 1101 7 0.2233277737546544 1101 8 0.16923067126478486 1101 9 0.2684138036268706 1101 10-11 0.21948501548317978 1101 12-13 0.21697091895022425 1101 14-15 0.2923184414200364 1101 16-17 0.343780408679784 1101 18-19 0.3646386516269615 1101 20-21 0.3639428361030852 1101 22-23 0.37911521119019653 1101 24-25 0.36018837432243345 1101 26-27 0.28462401915847124 1101 28-29 0.2692929987027384 1101 30-31 0.30831908909398464 1101 32-33 0.27547049620816466 1101 34-35 0.27191452833667284 1101 36-37 0.2657473131240664 1101 38-39 0.17677081750098722 1101 40-41 -0.11679992752505086 1101 42-43 -0.07703689794895041 1101 44-45 -0.01131335153949209 1101 46-47 0.1219992227342459 1101 48-49 0.12960162597921254 1101 50-51 0.1640320749103381 1101 52-53 0.05297343432481938 1101 54-55 0.04571999828339557 1101 56-57 -0.07730092545100575 1101 58-59 -0.03598406004764598 1101 60-61 -0.07708992720336028 1101 62-63 -0.08646708448018714 1101 64-65 -0.27957537947211364 1101 66-67 -0.2075842302634925 1101 68-69 -0.22670808203464432 1101 70-71 -0.21975683943277957 1101 72-73 -0.24405918635763513 1101 74-75 -0.25822745584758877 1101 76-77 -0.28317132282498036 1101 78-79 -0.27871888369254805 1101 80-81 -0.2606996314234067 1101 82-83 -0.3041705102269141 1101 84-85 -0.2962163272839504 1101 86-87 -0.34608251702795556 1101 88-89 -0.3206962020302697 1101 90-91 -0.2685682992174776 1101 92-93 -0.21587899983328995 1101 94-95 -0.20440294449964824 1101 96-97 -0.2630073053337938 1101 98-99 -0.30409425449109406 1101 100-101 -0.2284524532363683 1102 1 0.2146976110466099 1102 2 0.23878032972744734 1102 3 0.22521893031895246 1102 4 0.18184017221248894 1102 5 0.18915910728482999 1102 6 0.21529567283201345 1102 7 0.19051078015809964 1102 8 0.1479985877769181 1102 9 0.23821199078731325 1102 10-11 0.22210094293426863 1102 12-13 0.20407244914089517 1102 14-15 0.3023866423849739 1102 16-17 0.28483971536319785 1102 18-19 0.34857909576347623 1102 20-21 0.31387737408821437 1102 22-23 0.3066397970131618 1102 24-25 0.2758693194779056 1102 26-27 0.27101166749633165 1102 28-29 0.2944469458706749 1102 30-31 0.24437134021631834 1102 32-33 0.2838313631632374 1102 34-35 0.2986012311677584 1102 36-37 0.2857787759784145 1102 38-39 0.2121633663011906 1102 40-41 -0.20853586089148024 1102 42-43 -0.15010959547606006 1102 44-45 -0.1354759774841483 1102 46-47 -0.03300682087654394 1102 48-49 0.11800589620973767 1102 50-51 0.16586292439082229 1102 52-53 0.10431094365659987 1102 54-55 0.03976674805138458 1102 56-57 0.0369846468691577 1102 58-59 0.019104089452675055 1102 60-61 -0.06308997150579998 1102 62-63 -0.1029158393007421 1102 64-65 -0.13543399500701625 1102 66-67 -0.07674240878817429 1102 68-69 -0.14208899366038352 1102 70-71 -0.07753997542339874 1102 72-73 -0.11690806011111832 1102 74-75 -0.13468667800777112 1102 76-77 -0.18639863747172924 1102 78-79 -0.2346152366812504 1102 80-81 -0.20274754627106972 1102 82-83 -0.21458326207308076 1102 84-85 -0.2579555297743923 1102 86-87 -0.33509217636420274 1102 88-89 -0.31985518334627727 1102 90-91 -0.23851989076483093 1102 92-93 -0.24445133156914167 1102 94-95 -0.2238535851183343 1102 96-97 -0.2093196768389518 1102 98-99 -0.21273289714626387 1102 100-101 -0.15335629934465445 1103 1 0.1424341644132241 1103 2 0.16552262630880676 1103 3 0.18157600009418218 1103 4 0.13949189130260464 1103 5 0.13419083689971245 1103 6 0.170150733362334 1103 7 0.21228078123931482 1103 8 0.12579452493375243 1103 9 0.23168875857064108 1103 10-11 0.20011784204631766 1103 12-13 0.1827223401628828 1103 14-15 0.22794770834455136 1103 16-17 0.25443627463624097 1103 18-19 0.33185730566478355 1103 20-21 0.29723434102857027 1103 22-23 0.2608923484031891 1103 24-25 0.27162936150448047 1103 26-27 0.23900116507233804 1103 28-29 0.21520627030675143 1103 30-31 0.2041062977870638 1103 32-33 0.2838522830017354 1103 34-35 0.2630275276517082 1103 36-37 0.23041635821390116 1103 38-39 0.20787251616622626 1103 40-41 -0.09481351771434987 1103 42-43 -0.168313523408834 1103 44-45 -0.09665547563745491 1103 46-47 0.0034536342872897308 1103 48-49 0.10109543784597719 1103 50-51 0.12056183485800887 1103 52-53 0.00986825544028136 1103 54-55 -0.02299117468562173 1103 56-57 -0.06724531058696215 1103 58-59 -0.016547521770114315 1103 60-61 0.0033222109135984113 1103 62-63 -0.11062342200575515 1103 64-65 -0.13957125366595946 1103 66-67 -0.14721420267816043 1103 68-69 -0.2034059002262225 1103 70-71 -0.25258880504177483 1103 72-73 -0.3223372692838673 1103 74-75 -0.37243151170782696 1103 76-77 -0.285217649153239 1103 78-79 -0.33253916436256503 1103 80-81 -0.3317804662559034 1103 82-83 -0.31123625396708476 1103 84-85 -0.2784780967923055 1103 86-87 -0.29567680936800755 1103 88-89 -0.29572052250047065 1103 90-91 -0.35623905412650103 1103 92-93 -0.34890733003005536 1103 94-95 -0.33239404384453763 1103 96-97 -0.35535344024253845 1103 98-99 -0.3732211336861866 1103 100-101 -0.32716384779340757 1104 1 0.1333025749877308 1104 2 0.12860940561331802 1104 3 0.1056221566173079 1104 4 0.09891943117011692 1104 5 0.14209701020096333 1104 6 0.16516205031022224 1104 7 0.14130433192092084 1104 8 0.12802310708869413 1104 9 0.1859085570927803 1104 10-11 0.1948729330265664 1104 12-13 0.15692884769265447 1104 14-15 0.2174506709567794 1104 16-17 0.16693243985619688 1104 18-19 0.26350278588910925 1104 20-21 0.2377621142771673 1104 22-23 0.25511784412084637 1104 24-25 0.23668323472556807 1104 26-27 0.15280727452750398 1104 28-29 0.1857680862892579 1104 30-31 0.1751542207532637 1104 32-33 0.22743298382498978 1104 34-35 0.2442347325394536 1104 36-37 0.22597393078191885 1104 38-39 0.19996281088598522 1104 40-41 -0.03676971398480333 1104 42-43 -0.02772146562259792 1104 44-45 -0.0027303014483095467 1104 46-47 0.09320452166656423 1104 48-49 0.1549670756329462 1104 50-51 0.21276730905083951 1104 52-53 0.10695256015883103 1104 54-55 0.10849163627711533 1104 56-57 0.07527284328112671 1104 58-59 0.07987182595559972 1104 60-61 0.11920459401382999 1104 62-63 0.12957126672433716 1104 64-65 0.1498150620850005 1104 66-67 0.15382271328076413 1104 68-69 0.1102818589542629 1104 70-71 0.08643688011427031 1104 72-73 0.03839590000885096 1104 74-75 0.06607691869347221 1104 76-77 0.11124064777537157 1104 78-79 0.025793401538788174 1104 80-81 0.0445765486340477 1104 82-83 0.030942358706500528 1104 84-85 0.051502379133339105 1104 86-87 0.10550426845053806 1104 88-89 0.09521231876253111 1104 90-91 0.09048746825892096 1104 92-93 0.1652856944953669 1104 94-95 0.11517035551392141 1104 96-97 0.09804242443413358 1104 98-99 0.16495516637690955 1104 100-101 0.19686865706392354 1105 1 0.12107579105998667 1105 2 0.17138100623408548 1105 3 0.11277313180517723 1105 4 0.10341381655008064 1105 5 0.06257533120224679 1105 6 0.14291474341500532 1105 7 0.12921687838127127 1105 8 0.140560631593182 1105 9 0.23007350252826342 1105 10-11 0.1848324527964138 1105 12-13 0.19487674456230053 1105 14-15 0.2793059829104294 1105 16-17 0.27327602905169357 1105 18-19 0.3016822635520171 1105 20-21 0.27044353901655427 1105 22-23 0.2761291003772186 1105 24-25 0.2740941950634834 1105 26-27 0.22193612148893038 1105 28-29 0.19540898941443885 1105 30-31 0.20335883912296282 1105 32-33 0.2258041613214985 1105 34-35 0.228573267068505 1105 36-37 0.2447983205012747 1105 38-39 0.24460159343687593 1105 40-41 0.017045727406355127 1105 42-43 -0.017027303683747164 1105 44-45 -0.006520662714670777 1105 46-47 0.12675109609688207 1105 48-49 0.21712891403106482 1105 50-51 0.2676245641717898 1105 52-53 0.10860296499592437 1105 54-55 0.13359306915167934 1105 56-57 0.08167110158169066 1105 58-59 0.06603061593352066 1105 60-61 0.1262977829895604 1105 62-63 0.04563875022996555 1105 64-65 0.04363194557310379 1105 66-67 0.0017890721022375544 1105 68-69 0.001053177377574599 1105 70-71 6.146128521606897E-4 1105 72-73 -0.09038576999344272 1105 74-75 -0.060944289873816615 1105 76-77 -0.06195436405100807 1105 78-79 -0.06901896887766767 1105 80-81 -0.10658171145614048 1105 82-83 -0.08010801714129201 1105 84-85 -0.0378528025485636 1105 86-87 -0.07803550892620947 1105 88-89 -0.038374896767678024 1105 90-91 -0.009350176103762209 1105 92-93 0.06482322673634755 1105 94-95 0.1730660204755452 1105 96-97 0.0868323863915137 1105 98-99 0.14190600978257706 1105 100-101 0.13244349806792854 1106 1 0.028591058367155142 1106 2 0.04347328007622764 1106 3 0.021862158787072872 1106 4 0.019200017933947322 1106 5 0.027992854767461495 1106 6 0.05903319368177762 1106 7 0.02383434510584692 1106 8 0.019248158552770178 1106 9 0.041428932803711405 1106 10-11 0.07566384451197905 1106 12-13 0.03179746643521497 1106 14-15 0.056180553517883425 1106 16-17 0.07579026570656566 1106 18-19 0.1041721788562242 1106 20-21 0.06583879412137605 1106 22-23 0.07885033095902827 1106 24-25 0.06697510933631179 1106 26-27 0.06795308460928595 1106 28-29 0.03507782156949446 1106 30-31 0.06478365837214994 1106 32-33 0.08265443702324404 1106 34-35 0.12299868928284496 1106 36-37 0.09676996565292484 1106 38-39 0.06355850960661513 1106 40-41 -0.15361843238483885 1106 42-43 -0.12474728504165 1106 44-45 -0.08797722753705983 1106 46-47 -0.06345461184363899 1106 48-49 0.07808839011311619 1106 50-51 0.07643813482835071 1106 52-53 0.13113849345069184 1106 54-55 0.1067321788031208 1106 56-57 0.04463733551329341 1106 58-59 0.04072391598840852 1106 60-61 0.07139789329509938 1106 62-63 0.06190358137057572 1106 64-65 0.04027481785169584 1106 66-67 0.032435033753319686 1106 68-69 0.01102103452894454 1106 70-71 0.02142258014198717 1106 72-73 -0.07832538717177684 1106 74-75 -0.11242343769135488 1106 76-77 -0.04044082916927394 1106 78-79 -0.06909396438352644 1106 80-81 -0.10238246598296996 1106 82-83 -0.10376262623458032 1106 84-85 -0.08517549764831855 1106 86-87 -0.1461550243639671 1106 88-89 -0.18310968195475752 1106 90-91 -0.14324782621051924 1106 92-93 -0.05433107702782891 1106 94-95 -0.07680505132330495 1106 96-97 -0.11949909827208671 1106 98-99 -0.16838392696207904 1106 100-101 -0.11524886353141994 1107 1 0.04566033540938719 1107 2 0.019468299088615026 1107 3 0.020566915572260314 1107 4 0.03776169095394266 1107 5 0.07254528869398058 1107 6 0.05368156450328598 1107 7 0.03311430842870777 1107 8 0.0330636707461025 1107 9 0.06716593029125306 1107 10-11 0.05606011933858923 1107 12-13 0.05516982822530281 1107 14-15 0.02336496975836866 1107 16-17 0.05003546653136226 1107 18-19 0.031139000065685707 1107 20-21 0.04079566004418922 1107 22-23 0.09747292782788719 1107 24-25 0.10586030479336728 1107 26-27 0.08563935529218725 1107 28-29 0.1050017391919198 1107 30-31 0.10781484793690765 1107 32-33 0.0964112640085446 1107 34-35 0.09889060188921661 1107 36-37 0.06642486076462717 1107 38-39 0.10350250014090534 1107 40-41 -0.09414683827979076 1107 42-43 -0.0027903978888659253 1107 44-45 -0.011589746263602763 1107 46-47 -0.041882767791413755 1107 48-49 0.002424628608423518 1107 50-51 0.049539819935752405 1107 52-53 -0.01051250058124964 1107 54-55 -0.09365152111292474 1107 56-57 -0.14488900902708934 1107 58-59 -0.10322496473765597 1107 60-61 -0.10967587864103123 1107 62-63 -0.10434748338053623 1107 64-65 -0.17234188614870405 1107 66-67 -0.1091870161761257 1107 68-69 -0.21173325102468965 1107 70-71 -0.22009687791720722 1107 72-73 -0.2615925915232502 1107 74-75 -0.29017116559005274 1107 76-77 -0.3044577174602132 1107 78-79 -0.2605791763166714 1107 80-81 -0.2549392391846439 1107 82-83 -0.2273100677107074 1107 84-85 -0.21553995994292308 1107 86-87 -0.24764674718672453 1107 88-89 -0.20824339308158812 1107 90-91 -0.2413997391127154 1107 92-93 -0.14876878783854863 1107 94-95 -0.08479255508162709 1107 96-97 -0.17272822478825844 1107 98-99 -0.16995121973635108 1107 100-101 -0.0515613754185793 1108 1 0.003458483796904943 1108 2 0.06581247517831912 1108 3 0.07581468542016268 1108 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0.4554834887431696 2213 84-85 0.42359443915905004 2213 86-87 0.48715419105512936 2213 88-89 0.39702994408965964 2213 90-91 0.42242548395533674 2213 92-93 0.4572431023183796 2213 94-95 0.5038010635379564 2213 96-97 0.5829634187003734 2213 98-99 0.6483234517076966 2213 100-101 0.5041297266147318 2214 1 -0.12666532251497387 2214 2 -0.08202307791648167 2214 3 -0.11466161288527843 2214 4 -0.1455558226575988 2214 5 -0.16634592057122433 2214 6 -0.18017998667123436 2214 7 -0.18369513913467728 2214 8 -0.14689979949469745 2214 9 -0.16871001454157408 2214 10-11 -0.19186608767324032 2214 12-13 -0.19634297344281038 2214 14-15 -0.2532560978993246 2214 16-17 -0.23589728444240876 2214 18-19 -0.21464028349186748 2214 20-21 -0.26808086755441707 2214 22-23 -0.29723980823261087 2214 24-25 -0.2610182896550839 2214 26-27 -0.20144831153874065 2214 28-29 -0.16093500051120913 2214 30-31 -0.17642029698889417 2214 32-33 -0.16757637193190789 2214 34-35 -0.13063489341391943 2214 36-37 -0.13156149454813715 2214 38-39 -0.09614800259463863 2214 40-41 0.14828269685411044 2214 42-43 0.08957867017299037 2214 44-45 0.08182865001317197 2214 46-47 -0.009945759840988444 2214 48-49 0.01341659184559063 2214 50-51 0.02212588318224107 2214 52-53 0.02671510465307847 2214 54-55 -0.04088452754294991 2214 56-57 -0.23619642294008258 2214 58-59 0.08390434262792468 2214 60-61 0.20241231024811768 2214 62-63 0.24196794462868 2214 64-65 0.29289969591544107 2214 66-67 0.31878417515241964 2214 68-69 0.2692349956415825 2214 70-71 0.18475620530718118 2214 72-73 0.18569745166311336 2214 74-75 0.21416876913338 2214 76-77 0.1625749791962292 2214 78-79 0.20100363975939928 2214 80-81 0.14984001538024927 2214 82-83 0.17560527149818483 2214 84-85 0.12644625131633092 2214 86-87 0.16149439640422614 2214 88-89 0.11922114364958247 2214 90-91 0.2464289785241931 2214 92-93 0.25470845598374225 2214 94-95 0.21256225506058968 2214 96-97 0.2144049892865958 2214 98-99 0.22521141555827384 2214 100-101 0.16753347414363162 2215 1 -0.10675784780944042 2215 2 -0.12230048383664638 2215 3 -0.1184355824007497 2215 4 -0.13878709777254272 2215 5 -0.16078155894108193 2215 6 -0.19864610113349812 2215 7 -0.1968926749506963 2215 8 -0.16964906265633317 2215 9 -0.311732156779307 2215 10-11 -0.2478614511888182 2215 12-13 -0.2601175715405617 2215 14-15 -0.35236810947267827 2215 16-17 -0.33558834167143203 2215 18-19 -0.3012625309290229 2215 20-21 -0.38315938995964416 2215 22-23 -0.27678105792371355 2215 24-25 -0.19305342166344275 2215 26-27 -0.17798100378117 2215 28-29 -0.15052310268144709 2215 30-31 -0.1919860279342771 2215 32-33 -0.3012907513660892 2215 34-35 -0.25045335558636594 2215 36-37 -0.3312747712620876 2215 38-39 -0.28090004624841214 2215 40-41 0.13154074518541137 2215 42-43 0.01381404367752026 2215 44-45 -0.019269155056228726 2215 46-47 -0.08995929824229876 2215 48-49 -0.3357560689022492 2215 50-51 -0.4320921885342557 2215 52-53 -0.2485793869388715 2215 54-55 -0.42773482025830134 2215 56-57 -0.32224252786894425 2215 58-59 -0.16817537746722167 2215 60-61 -0.3017904095311472 2215 62-63 -0.6366752349674378 2215 64-65 -0.4221309666576296 2215 66-67 -0.13791340665853014 2215 68-69 -0.0817377886335322 2215 70-71 -0.046940584359273174 2215 72-73 -0.02071108856949877 2215 74-75 -0.044816696577768766 2215 76-77 0.06619942738812057 2215 78-79 0.045400432139317104 2215 80-81 0.08052683184496345 2215 82-83 0.046084702076139905 2215 84-85 0.014199175589382662 2215 86-87 0.01082034959366851 2215 88-89 -0.10883967851179932 2215 90-91 0.04540127092633384 2215 92-93 0.07595792222327447 2215 94-95 -0.014927731639801323 2215 96-97 0.0014191879121376871 2215 98-99 0.008477665007031732 2215 100-101 -0.05133187363356129 2216 1 -0.20330860840635978 2216 2 -0.17580547794569412 2216 3 -0.20322986308317326 2216 4 -0.2195214667472527 2216 5 -0.1970491831297707 2216 6 -0.2313713053967419 2216 7 -0.32207292636556417 2216 8 -0.1812728747986796 2216 9 -0.3344849517149555 2216 10-11 -0.3558118410477391 2216 12-13 -0.33381494831991176 2216 14-15 -0.49672105273552347 2216 16-17 -0.4474051231792302 2216 18-19 -0.4244846953113992 2216 20-21 -0.37026008257991805 2216 22-23 -0.3961183137585351 2216 24-25 -0.451361778229014 2216 26-27 -0.353575797981847 2216 28-29 -0.38697033692055527 2216 30-31 -0.38855605613297683 2216 32-33 -0.5757692873910472 2216 34-35 -0.6144278991508756 2216 36-37 -0.6546940505444496 2216 38-39 -0.6030406021367227 2216 40-41 0.21249578487000775 2216 42-43 0.13559302335066548 2216 44-45 0.023762734758157933 2216 46-47 -0.12486301623607687 2216 48-49 -0.5579830476516179 2216 50-51 -0.7347846032061085 2216 52-53 -0.6579818686059866 2216 54-55 -0.624224891243081 2216 56-57 -0.7301864840822816 2216 58-59 -0.584042615557177 2216 60-61 -0.5918985054440711 2216 62-63 -0.5710757925411691 2216 64-65 -0.46287424139789834 2216 66-67 -0.40748267242022607 2216 68-69 -0.4792640512414792 2216 70-71 -0.4274416060088555 2216 72-73 -0.363239348607717 2216 74-75 -0.3552174440273781 2216 76-77 -0.26889517186976164 2216 78-79 -0.3012598124378876 2216 80-81 -0.20476183149816762 2216 82-83 -0.2936555733185564 2216 84-85 -0.3239063978011103 2216 86-87 -0.3153275767856947 2216 88-89 -0.29920741102574766 2216 90-91 -0.271200544148428 2216 92-93 -0.2540814436872836 2216 94-95 -0.3700941490499261 2216 96-97 -0.39085851463235954 2216 98-99 -0.3278453405361681 2216 100-101 -0.4554893434094289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 32.0 11 704.0 12 2592.0 13 5491.0 14 8878.0 15 13443.0 16 20651.0 17 36702.0 18 90565.0 19 298107.0 20 319423.0 21 202528.0 22 169161.0 23 154398.0 24 152995.0 25 165714.0 26 196071.0 27 238542.0 28 284079.0 29 316834.0 30 343535.0 31 389047.0 32 457729.0 33 543540.0 34 658565.0 35 1020807.0 36 1309559.0 37 1498078.0 38 1464607.0 39 251475.0 40 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.79417182104683 35.74500539384489 15.45968277596936 12.001140009138917 2 13.40024213417248 19.127365401193714 57.37240141511878 10.099991049515026 3 13.300882329387267 34.71928923727736 15.966798411524454 36.01303002181092 4 38.58003853419321 17.28071076272264 31.27825173474531 12.860998968338839 5 14.630622906646442 40.92361467691104 14.685597727518973 29.760164688923542 6 14.910697713857706 16.403883568322822 30.08879823251476 38.59662048530472 7 39.23106854658256 16.87092929587948 14.248889433245871 29.649112724292088 8 55.02747798887313 16.869949453313797 14.413926954621042 13.688645603192025 9 14.77182387329882 32.1342761177507 14.76258131986678 38.3313186890837 10-11 42.956839819294416 16.234587500412193 14.578017608901492 26.23055507139189 12-13 34.482751473296936 16.403911833012216 14.815648274205174 34.29768841948568 14-15 34.08310760838331 16.65324407972527 15.089839315240791 34.173808996650635 16-17 33.702608359752965 28.772148917226858 15.173988006350134 22.351254716670045 18-19 21.902378473612558 16.9884397420376 27.56704148785325 33.54214029649659 20-21 20.890389534527674 36.898666377738735 16.695372599267944 25.51557148846565 22-23 12.722087441527425 28.29712783647935 37.10302479284338 21.877759929149846 24-25 12.71190273178223 16.78891458881943 49.50669637599574 20.992486303402597 26-27 12.699485111574862 16.753729761304697 57.7846795961918 12.762105530928636 28-29 12.78578662985976 16.26328558171086 58.28525666693361 12.665671121495766 30-31 12.740841062940753 16.179122758256824 58.35881081030154 12.721225368500889 32-33 12.733496954479717 16.16815605877171 58.373824071151645 12.724522915596928 34-35 12.803678178246553 16.23475708854856 58.26251772431564 12.699047008889245 36-37 12.850847234064602 16.19375915658167 58.11074105304811 12.844652556305617 38-39 13.5491028316508 16.65331945223032 55.854785447453594 13.942792268665293 40-41 17.720584702207944 19.83955077986989 44.20650182071707 18.233362697205095 42-43 17.76984063425963 20.727127977802798 45.257576114453144 16.245455273484424 44-45 18.92369947097923 22.71862972785815 42.05078693606056 16.30688386510206 46-47 18.29507864649824 24.57768785419189 40.67896965785594 16.448263841453937 48-49 18.22469014834251 26.245681390999582 38.19283584339478 17.33679261726313 50-51 18.522402121736018 26.246355032763486 37.13703663574824 18.094206209752258 52-53 18.592903678649325 25.867160670626866 37.14522397410954 18.394711676614268 54-55 18.663702014801274 24.96809387645504 37.43940757211029 18.928796536633392 56-57 18.60412004955742 24.816920185227932 37.82084426627222 18.75811549894243 58-59 18.76618035698303 24.953528139853685 37.76076766896396 18.51952383419933 60-61 19.17996127737553 25.131986677909733 36.997635187653984 18.690416857060754 62-63 19.639648764126456 25.387466494566112 36.15424983158956 18.818634909717872 64-65 19.75045576811649 25.57159681362735 35.35858469278638 19.319362725469784 66-67 19.830463682229517 25.706282769374265 34.70614615670886 19.757107391687352 68-69 19.93553766505401 25.99094587783059 34.33447962351433 19.739036833601062 70-71 20.25235185769671 26.258452319824382 33.88256492634693 19.60663089613198 72-73 20.375675408306993 26.437438465415795 33.65453012309273 19.53235600318449 74-75 20.62395501236979 26.575652440670893 33.321323768141774 19.47906877881754 76-77 20.742444448642384 26.770128841867557 32.93116014211673 19.556266567373328 78-79 20.56866084523304 26.728894225195194 32.95834296947952 19.744101960092244 80-81 20.510740630588668 26.663126131631298 32.990919342651445 19.835213895128586 82-83 20.490818169724136 26.553041195094533 33.11769558615642 19.838445049024912 84-85 20.473752477530166 26.24076246453117 33.436602032222474 19.84888302571619 86-87 20.328861097768918 26.220498027941407 33.60479798812525 19.845842886164423 88-89 20.399859598219322 26.194617576857652 33.42443306609855 19.98108975882447 90-91 20.602646284661088 26.12160190315799 33.12470984495622 20.151041967224696 92-93 20.732343962147162 26.179709584003007 32.95896898674923 20.128977467100597 94-95 20.6908785136542 26.23048440966841 32.97236184455363 20.10627523212376 96-97 20.658001969106696 26.27307458580453 32.707997493864205 20.36092595122457 98-99 20.802359159408137 25.981194559989447 32.81171948237932 20.404726798223095 100-101 20.948336858568204 25.805562490872862 32.469542441786515 20.776558208772418 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 156738.0 1 84832.5 2 9032.0 3 4134.5 4 2867.5 5 2503.0 6 2325.5 7 2246.5 8 2202.5 9 2173.0 10 2443.0 11 2792.0 12 3728.0 13 6096.0 14 8167.5 15 19146.5 16 422103.5 17 428997.5 18 29551.5 19 13581.0 20 11209.5 21 11922.0 22 14353.0 23 18139.0 24 23313.5 25 30529.0 26 40746.0 27 54792.0 28 73785.0 29 97178.0 30 125085.0 31 158645.0 32 198167.0 33 238156.0 34 272773.0 35 302828.0 36 325263.0 37 341456.0 38 348935.5 39 349280.0 40 353924.0 41 366366.0 42 383842.0 43 397821.0 44 403415.5 45 404743.5 46 401729.0 47 389312.0 48 374822.0 49 358520.0 50 333654.5 51 303695.0 52 267397.0 53 233732.5 54 207141.5 55 183201.5 56 163314.0 57 144373.0 58 128602.5 59 113984.5 60 97041.0 61 82294.5 62 71418.5 63 61511.5 64 51967.5 65 43079.5 66 35020.5 67 27684.0 68 21030.5 69 15532.0 70 10909.5 71 7286.5 72 4763.5 73 3025.5 74 1825.0 75 978.5 76 504.0 77 278.5 78 157.5 79 65.0 80 20.5 81 12.0 82 4.0 83 4.0 84 6.0 85 3.5 86 3.0 87 2.0 88 1.5 89 3.0 90 3.0 91 2.5 92 2.5 93 2.0 94 3.0 95 4.5 96 2.0 97 2.5 98 6.0 99 7.5 100 23.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.001771253868729361 76-77 0.002642748458396733 78-79 0.006590383410511638 80-81 0.006373687458486238 82-83 0.0015262932273093428 84-85 4.993428459715752E-4 86-87 0.0015828226060985778 88-89 0.0011729846098766246 90-91 1.1776953914423942E-4 92-93 2.826468939461746E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.061395E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.58281931137142 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25704777304654 26.717665236652582 2 10.384398342986536 7.182435431056164 3 3.4332958342079287 3.5619914843069096 4 1.8765628934406555 2.5958734188113013 5 1.1754107576543593 2.0324508924301448 6 0.8089426023071783 1.6785309509315842 7 0.639809670921061 1.5488495570191745 8 0.4980292231012882 1.3778603707435628 9 0.43226337966319023 1.3453997718433366 >10 3.1176383288605036 21.069168310159153 >50 0.24575552962804847 5.8742054702011055 >100 0.1243504023956755 6.926513255948503 >500 0.0030026434414778982 0.7080989124225677 >1k 0.0025376056392740804 1.771438049463176 >5k 4.6386331450433947E-4 1.155285574573316 >10k+ 4.911493918281242E-4 14.454233313437626 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 965379 9.095379194362136 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 172154 1.6219597793469913 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 48661 0.45846268354382674 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37069 0.34924792372302493 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 35145 0.3311208362579436 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 32641 0.3075292421765695 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC 31435 0.2961668370399333 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 30115 0.2837303737063016 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT 26987 0.25425972423084714 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 24762 0.2332967462631725 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 23473 0.22115235138661857 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22173 0.20890431931561765 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 19108 0.18002722831745016 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA 15203 0.14323602428878973 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC 12891 0.12145337032867123 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG 12319 0.11606423621743082 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT 11828 0.11143824871984512 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.747153510238884E-4 0.0 0.0 0.0 0.0 2 5.747153510238884E-4 0.0 0.0 0.0 0.0 3 6.12401603550045E-4 0.0 0.0 0.0 0.0 4 6.312447298131233E-4 0.0 0.0 0.0 0.0 5 6.689309823392799E-4 0.0 0.0 0.0 0.0 6 7.631466136546715E-4 0.0 0.0 0.0 0.0 7 8.102544293123672E-4 0.0 0.0 0.0 0.0 8 8.385191187069847E-4 0.0 0.0 0.0 0.0 9 0.0010175288182062286 1.8843126263078307E-5 0.0 0.0 0.0 10-11 0.0011776953914423942 1.8843126263078307E-5 0.0 0.0 0.0 12-13 0.001356705090941638 1.8843126263078307E-5 0.0 0.0 0.0 14-15 0.0015215824457435732 1.8843126263078307E-5 0.0 0.0 0.0 16-17 0.0016534843295851216 1.8843126263078307E-5 0.0 0.0 0.0 18-19 0.0018843126263078307 1.8843126263078307E-5 0.0 0.0 0.0 20-21 0.002105719359899001 2.826468939461746E-5 0.0 0.0 0.0 22-23 0.0023648123460163276 2.826468939461746E-5 0.0 0.0 0.0 24-25 0.0026615915846598106 3.768625252615662E-5 0.0 0.0 0.0 26-27 0.002826468939461746 4.710781565769577E-5 0.0 0.0 0.0 28-29 0.003024321765224068 4.710781565769577E-5 0.0 0.0 0.0 30-31 0.0032363069356836993 5.181859722346535E-5 0.0 0.0 0.0 32-33 0.003518953829629874 5.652937878923492E-5 0.0 0.0 0.0 34-35 0.003778046815747201 5.652937878923492E-5 0.0 0.0 0.0 36-37 0.004060693709693376 5.652937878923492E-5 0.0 0.0 0.0 38-39 0.004324497477376472 5.652937878923492E-5 0.0 0.0 0.0 40-41 0.004658962968546111 5.652937878923492E-5 0.0 0.0 0.0 42-43 0.004790864852387659 5.652937878923492E-5 0.0 0.0 0.0 44-45 0.004965163770321134 5.652937878923492E-5 0.0 0.0 0.0 46-47 0.005163016596083456 5.652937878923492E-5 0.0 0.0 4.710781565769577E-6 48-49 0.005266653790530387 5.652937878923492E-5 0.0 0.0 9.421563131539153E-6 50-51 0.005483349742555788 5.652937878923492E-5 0.0 0.0 9.421563131539153E-6 52-53 0.006519721687025094 5.652937878923492E-5 0.0 0.0 9.421563131539153E-6 54-55 0.007881137559532502 6.12401603550045E-5 0.0 0.0 9.421563131539153E-6 56-57 0.009398009223710306 7.066172348654364E-5 0.0 0.0 9.421563131539153E-6 58-59 0.011131576839913511 8.008328661808281E-5 0.0 0.0 9.421563131539153E-6 60-61 0.013105394315970962 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 62-63 0.015295907744053816 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 64-65 0.018207170751699413 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 66-67 0.02158951191592197 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 68-69 0.026088308311231916 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 70-71 0.031110001460342283 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 72-73 0.03700789998068579 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 74-75 0.04417770952378709 8.479406818385238E-5 0.0 0.0 9.421563131539153E-6 76-77 0.053914895020232806 8.479406818385238E-5 0.0 4.710781565769577E-6 9.421563131539153E-6 78-79 0.06559763330334135 8.479406818385238E-5 0.0 9.421563131539153E-6 9.421563131539153E-6 80-81 0.07943790954357237 8.479406818385238E-5 0.0 9.421563131539153E-6 9.421563131539153E-6 82-83 0.0962177134808436 8.479406818385238E-5 0.0 1.413234469730873E-5 9.421563131539153E-6 84-85 0.11597944214924699 9.421563131539154E-5 0.0 1.8843126263078307E-5 9.421563131539153E-6 86-87 0.1395616146674895 9.421563131539154E-5 0.0 1.8843126263078307E-5 9.421563131539153E-6 88-89 0.1673834905949246 9.421563131539154E-5 0.0 2.3553907828847886E-5 9.421563131539153E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 257430 0.0 94.54299 1 TCGACGG 260515 0.0 93.42341 2 ATATCTC 176165 0.0 92.248505 1 CGACGGC 264685 0.0 91.8762 3 GACGGCG 266705 0.0 91.20883 4 ACGGCGC 266750 0.0 91.191666 5 TATCTCG 178510 0.0 90.89565 2 CGGCGCG 268835 0.0 90.450836 6 ATCTCGA 184545 0.0 87.86141 3 TCTCGAG 185730 0.0 86.50036 4 CTCGAGG 189870 0.0 84.71934 5 TCGAGGG 192850 0.0 83.48657 6 AGGGCGC 197205 0.0 81.98972 9 CGAGGGC 196855 0.0 81.85802 7 GAGGGCG 199185 0.0 81.13417 8 CTCGACG 2150 0.0 63.627064 5 TCTCGAC 2505 0.0 54.799667 4 GGCGCGC 467025 0.0 52.887257 7 CGCGCCG 467340 0.0 52.83433 9 GCGCGCC 467455 0.0 52.82438 8 >>END_MODULE