FastQCFastQC Report
Thu 26 May 2016
SRR1051298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10613950
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9498838.949382652075807No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT320600.3020553139971453No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318570.3001427366814428No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT316150.29786271840361034No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118260.11141940559358204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG67150.088.209831
TCGACGG74900.079.397662
TATCTCG111250.054.095592
ATATCTC117850.051.956831
CGACGGC115600.051.4436453
GACGGCG118600.050.102324
ACGGCGC122000.048.6281625
ATCTCGA193900.046.4479451
TCTCGAG213700.041.920112
CGGCGCG151400.039.4047856
CTCGAGG260350.034.682453
TCGAGGG265850.034.072134
AGGGCGC288800.031.3645297
GGGCGCG293300.030.8509248
CGAGGGC298050.030.4867465
GCGTAAT6050.029.04902528-29
GAGGGCG323050.028.1421626
TGCGTAA7800.022.83609826-27
CGCGCCG441500.022.1411349
GGCGCGC450300.021.7506357
Produced by FastQC (version 0.11.5)