##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10613950 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42208367290217 33.0 31.0 34.0 31.0 34.0 2 32.634853848001924 34.0 31.0 34.0 31.0 34.0 3 32.66672727872281 34.0 31.0 34.0 31.0 34.0 4 36.162865568426454 37.0 35.0 37.0 35.0 37.0 5 35.930021810918646 37.0 35.0 37.0 35.0 37.0 6 35.79878989443139 37.0 35.0 37.0 33.0 37.0 7 35.668960848694404 37.0 35.0 37.0 33.0 37.0 8 35.61521978151395 37.0 35.0 37.0 33.0 37.0 9 37.152801925767506 39.0 37.0 39.0 33.0 39.0 10-11 37.13116191427319 39.0 37.0 39.0 33.0 39.0 12-13 37.18813363545146 39.0 37.0 39.0 33.0 39.0 14-15 38.60120200302432 40.0 38.0 41.0 34.0 41.0 16-17 38.66169272514003 40.0 38.0 41.0 34.0 41.0 18-19 38.502398871296734 40.0 37.0 41.0 35.0 41.0 20-21 38.554529652014565 40.0 37.0 41.0 35.0 41.0 22-23 38.56201927651817 40.0 37.0 41.0 35.0 41.0 24-25 38.50096472095686 40.0 37.0 41.0 35.0 41.0 26-27 38.31872596912554 40.0 37.0 41.0 35.0 41.0 28-29 38.1160078952699 40.0 36.5 41.0 34.5 41.0 30-31 38.005218698034184 40.0 36.0 41.0 34.5 41.0 32-33 38.07537994808719 40.0 36.0 41.0 35.0 41.0 34-35 38.08678757672685 40.0 36.0 41.0 35.0 41.0 36-37 38.08734481507827 40.0 36.0 41.0 35.0 41.0 38-39 38.05564883949896 40.0 36.0 41.0 35.0 41.0 40-41 37.98078495753231 40.0 36.0 41.0 34.0 41.0 42-43 37.909100429152204 40.0 35.5 41.0 34.0 41.0 44-45 37.78333805981751 40.0 35.0 41.0 33.5 41.0 46-47 37.57654054334155 40.0 35.0 41.0 33.0 41.0 48-49 37.431250382751 40.0 35.0 41.0 33.0 41.0 50-51 37.365713188775146 39.0 35.0 41.0 33.0 41.0 52-53 37.198816227700334 39.0 35.0 41.0 33.0 41.0 54-55 37.02510243594514 39.0 35.0 40.0 33.0 41.0 56-57 36.8986888481668 39.0 35.0 40.0 33.0 41.0 58-59 36.64874825112234 38.0 35.0 40.0 32.0 41.0 60-61 36.35487203161877 38.0 35.0 40.0 32.0 41.0 62-63 36.02664752519091 37.0 35.0 40.0 31.0 41.0 64-65 35.680531800130964 36.5 35.0 40.0 31.0 41.0 66-67 35.27091002878288 36.0 34.5 39.0 30.5 41.0 68-69 34.85066064000678 35.5 34.0 39.0 30.0 40.5 70-71 34.55023704652839 35.0 34.0 38.0 30.0 40.0 72-73 34.21230197051993 35.0 34.0 37.0 29.5 39.5 74-75 33.91017378073197 35.0 34.0 37.0 29.0 39.0 76-77 33.65654831613113 35.0 34.0 36.0 29.0 39.0 78-79 33.394395536063385 35.0 33.0 36.0 29.0 38.0 80-81 33.17023096962017 35.0 33.0 35.5 29.0 37.0 82-83 32.95179730449079 35.0 33.0 35.0 29.0 37.0 84-85 32.73395969455292 35.0 33.0 35.0 29.0 36.0 86-87 32.54223375840286 35.0 33.0 35.0 29.0 36.0 88-89 32.34994771032462 35.0 33.0 35.0 29.0 36.0 90-91 32.16784265047414 35.0 33.0 35.0 28.5 35.5 92-93 31.980478238544556 35.0 33.0 35.0 27.0 35.0 94-95 31.809161857743817 35.0 33.0 35.0 27.0 35.0 96-97 31.667248196948357 35.0 33.0 35.0 27.0 35.0 98-99 31.476083503314037 35.0 33.0 35.0 26.5 35.0 100-101 30.15930525393468 34.0 31.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18315633293728695 1101 2 0.20342176158377612 1101 3 0.21465532544608124 1101 4 0.15330666773425605 1101 5 0.20756049397221688 1101 6 0.2471283392921393 1101 7 0.27208639066702744 1101 8 0.2617585814495911 1101 9 0.3282159074936146 1101 10-11 0.2877184935594741 1101 12-13 0.2813023761864244 1101 14-15 0.280017813865328 1101 16-17 0.30601138243716974 1101 18-19 0.3288562636316925 1101 20-21 0.29880187014870074 1101 22-23 0.3295690281189749 1101 24-25 0.3179530198365157 1101 26-27 0.35727931441047645 1101 28-29 0.40063132582602634 1101 30-31 0.41824891907688766 1101 32-33 0.41890826891505384 1101 34-35 0.4190478147824592 1101 36-37 0.3741260147880041 1101 38-39 0.3911599020305516 1101 40-41 0.3326647967885279 1101 42-43 0.3374444438087991 1101 44-45 0.32907437260049477 1101 46-47 0.3560121599696515 1101 48-49 0.30047615887792034 1101 50-51 0.2996940682811342 1101 52-53 0.31736310117658206 1101 54-55 0.36633774042258693 1101 56-57 0.4199201386862583 1101 58-59 0.4009993562823695 1101 60-61 0.40306774848259863 1101 62-63 0.41254452251186535 1101 64-65 0.4627089656156187 1101 66-67 0.48452627341850985 1101 68-69 0.5133963342809622 1101 70-71 0.4253239617259652 1101 72-73 0.41933517340667237 1101 74-75 0.4404788427398927 1101 76-77 0.39618631595269704 1101 78-79 0.40752440384872557 1101 80-81 0.37585172517725596 1101 82-83 0.3760140922878321 1101 84-85 0.3986375098717829 1101 86-87 0.4106265787106551 1101 88-89 0.38787758236253467 1101 90-91 0.38902629330673477 1101 92-93 0.41023750154335303 1101 94-95 0.4394970098558808 1101 96-97 0.42667015945714226 1101 98-99 0.48075534087673333 1101 100-101 0.5431401801974793 1102 1 0.178268185765468 1102 2 0.20059530659047908 1102 3 0.2316769976983295 1102 4 0.14614963663935754 1102 5 0.23373847602556452 1102 6 0.25052194757721935 1102 7 0.21138692605980225 1102 8 0.19942003236428008 1102 9 0.2790056576962243 1102 10-11 0.2601249593159878 1102 12-13 0.20480101594247913 1102 14-15 0.28032342539027155 1102 16-17 0.28675385636997675 1102 18-19 0.30527878017201004 1102 20-21 0.2874902944967559 1102 22-23 0.24313395222642242 1102 24-25 0.33821658186565173 1102 26-27 0.3671065574050587 1102 28-29 0.40219908515419434 1102 30-31 0.41921305555872124 1102 32-33 0.3532111723222684 1102 34-35 0.3647364576982355 1102 36-37 0.32744880939804233 1102 38-39 0.3328682825966922 1102 40-41 0.3081080610511222 1102 42-43 0.33892662734097456 1102 44-45 0.33622237990793025 1102 46-47 0.3966243902315938 1102 48-49 0.40362694436117863 1102 50-51 0.3811708648702634 1102 52-53 0.3754577202883027 1102 54-55 0.370775709325315 1102 56-57 0.3496343086741476 1102 58-59 0.3983121290647347 1102 60-61 0.42953043462645724 1102 62-63 0.4097375809830339 1102 64-65 0.36126287427190107 1102 66-67 0.42753042840516287 1102 68-69 0.49769552367832404 1102 70-71 0.3976431658077786 1102 72-73 0.3516787705670694 1102 74-75 0.3611236176953554 1102 76-77 0.31628550713366366 1102 78-79 0.3298513938557335 1102 80-81 0.28502174538174074 1102 82-83 0.2200593875888046 1102 84-85 0.26892557404337225 1102 86-87 0.2819529495665378 1102 88-89 0.34727082010120114 1102 90-91 0.32753662963433783 1102 92-93 0.34435731048375473 1102 94-95 0.3660433777733907 1102 96-97 0.3786202765015467 1102 98-99 0.31809517520659725 1102 100-101 0.4105806788743962 1103 1 0.15582912686260642 1103 2 0.17353069072684235 1103 3 0.17212001570128166 1103 4 0.10547518426553637 1103 5 0.15721496914202504 1103 6 0.17224770204568074 1103 7 0.1978557449229683 1103 8 0.14009907155619317 1103 9 0.19204365337566287 1103 10-11 0.1609079517842389 1103 12-13 0.14686831739065553 1103 14-15 0.2079930530651879 1103 16-17 0.1912480116674331 1103 18-19 0.2481683009566069 1103 20-21 0.22064876929891142 1103 22-23 0.22499907861122637 1103 24-25 0.20697001170674412 1103 26-27 0.21152703801718076 1103 28-29 0.2047257793086743 1103 30-31 0.18109804217112924 1103 32-33 0.20594635786143556 1103 34-35 0.20098821052103233 1103 36-37 0.16883441174036307 1103 38-39 0.22794809967025742 1103 40-41 0.20080072693910722 1103 42-43 0.17153127520594325 1103 44-45 0.1675463341951584 1103 46-47 0.2313949922249563 1103 48-49 0.2247020967237674 1103 50-51 0.20257363499322167 1103 52-53 0.2085841568354141 1103 54-55 0.1687384940657708 1103 56-57 0.23192453880076158 1103 58-59 0.3204615669202724 1103 60-61 0.3332517695338737 1103 62-63 0.31375529262440693 1103 64-65 0.32817707266308815 1103 66-67 0.3377987725978855 1103 68-69 0.34795891073560625 1103 70-71 0.2705801588040373 1103 72-73 0.10840051991475974 1103 74-75 -0.09060688905213965 1103 76-77 -0.005921495966653367 1103 78-79 0.06402471529169418 1103 80-81 0.08545306259304652 1103 82-83 0.0762434595978192 1103 84-85 0.06524487746311536 1103 86-87 0.06117589674813928 1103 88-89 0.056053862538142596 1103 90-91 0.09373211963485772 1103 92-93 0.1379764191611308 1103 94-95 0.10606482873052059 1103 96-97 0.08382008970096422 1103 98-99 0.1396184446159765 1103 100-101 0.15416639629552975 1104 1 0.14495420131974868 1104 2 0.1350964260049068 1104 3 0.15829774817714792 1104 4 0.11904584553297326 1104 5 0.15707605023435178 1104 6 0.17158570586757094 1104 7 0.17875204746424345 1104 8 0.13576363242982126 1104 9 0.21106108823194347 1104 10-11 0.18075779555569937 1104 12-13 0.21016224621357793 1104 14-15 0.2598694502967547 1104 16-17 0.2847609690494721 1104 18-19 0.23487898428509624 1104 20-21 0.21554529902917352 1104 22-23 0.23754303432085777 1104 24-25 0.2334475363762465 1104 26-27 0.23246425675027638 1104 28-29 0.2217403300878118 1104 30-31 0.2535531643671831 1104 32-33 0.2510590615054298 1104 34-35 0.1973530733759219 1104 36-37 0.2170884852250765 1104 38-39 0.17589309181455803 1104 40-41 0.17234816245021278 1104 42-43 0.21336067049048069 1104 44-45 0.194433564228909 1104 46-47 0.15888124169733686 1104 48-49 0.19664939761481293 1104 50-51 0.14185058370741643 1104 52-53 0.14940628126610989 1104 54-55 0.23496415444673602 1104 56-57 0.252329709624739 1104 58-59 0.2466912760159019 1104 60-61 0.1799839178872702 1104 62-63 0.20430565961927982 1104 64-65 -0.04189142370701404 1104 66-67 -0.12000044095596252 1104 68-69 0.0513475140666273 1104 70-71 -0.008437295539025058 1104 72-73 0.07091401409041254 1104 74-75 0.13231047134111407 1104 76-77 0.07701884135691017 1104 78-79 0.08952349540243176 1104 80-81 0.058465043065808686 1104 82-83 -0.004593031965903549 1104 84-85 0.04706406805818375 1104 86-87 0.01763808196145078 1104 88-89 -0.006169704458599767 1104 90-91 -5.653066620183722E-4 1104 92-93 0.043570900027749104 1104 94-95 0.027008278193758173 1104 96-97 0.06634105218531516 1104 98-99 0.09788665542410513 1104 100-101 0.10642402299311726 1105 1 0.13680362740569052 1105 2 0.11830516579187389 1105 3 0.15284405928047562 1105 4 0.10892900538964767 1105 5 0.15450790194372388 1105 6 0.17461363870818047 1105 7 0.1571251615377136 1105 8 0.14641456830079846 1105 9 0.21838782077552565 1105 10-11 0.22546433783334408 1105 12-13 0.2082779560116066 1105 14-15 0.26606235202790174 1105 16-17 0.2656943271013503 1105 18-19 0.27199752191119586 1105 20-21 0.22645216310362315 1105 22-23 0.25417441948342656 1105 24-25 0.2352290357256237 1105 26-27 0.2421386334711073 1105 28-29 0.22708131601793724 1105 30-31 0.22018665352585032 1105 32-33 0.26198233183745145 1105 34-35 0.2453230425564712 1105 36-37 0.2190753247703725 1105 38-39 0.21751910748900372 1105 40-41 0.17484304132778306 1105 42-43 0.14168129518247952 1105 44-45 0.1547611621534557 1105 46-47 0.20563115511216523 1105 48-49 0.20919651085643665 1105 50-51 0.1995624928534241 1105 52-53 0.19554840382895122 1105 54-55 0.22036851241211508 1105 56-57 0.17378749402760008 1105 58-59 0.16692069304973955 1105 60-61 0.1954866360332943 1105 62-63 0.2117251317266522 1105 64-65 0.22911452753193373 1105 66-67 0.3161397405272055 1105 68-69 0.3331082726808461 1105 70-71 0.2601801104933017 1105 72-73 0.2691084272603135 1105 74-75 0.2858647409120607 1105 76-77 0.2672206072154708 1105 78-79 0.21788010497725452 1105 80-81 0.1514107411776564 1105 82-83 0.22749711256020788 1105 84-85 0.20530930542353332 1105 86-87 0.21863010384562642 1105 88-89 0.23881166924046227 1105 90-91 0.1663847459030876 1105 92-93 0.1754796710840374 1105 94-95 0.18438895940792932 1105 96-97 0.1846465141878646 1105 98-99 0.2033793009957634 1105 100-101 0.1494409110821806 1106 1 0.05207561853957543 1106 2 0.08240158323889091 1106 3 0.08167786393848075 1106 4 0.05096976024022837 1106 5 0.11903778035239299 1106 6 0.11851646341268918 1106 7 0.10250080630142833 1106 8 0.08154456962645185 1106 9 0.13137993570948936 1106 10-11 0.16583775991810512 1106 12-13 0.10275232945183888 1106 14-15 0.12572100647902573 1106 16-17 0.13942606432634364 1106 18-19 0.10549796870081707 1106 20-21 0.13223360659821992 1106 22-23 0.1473706418367442 1106 24-25 0.17608574147961775 1106 26-27 0.1577021402340364 1106 28-29 0.0785443083682722 1106 30-31 0.138288129292917 1106 32-33 0.16737914939873377 1106 34-35 0.16927926043899078 1106 36-37 0.12143648516805428 1106 38-39 0.08856821976872453 1106 40-41 0.07050699526899251 1106 42-43 0.029397656786152027 1106 44-45 0.1271692509378468 1106 46-47 0.13279825607388318 1106 48-49 0.11202600350323877 1106 50-51 0.09764665558521557 1106 52-53 0.030340196138403996 1106 54-55 0.12232796383223388 1106 56-57 0.1664089992583584 1106 58-59 0.13186643049363056 1106 60-61 0.16673888768650613 1106 62-63 0.16564864340274 1106 64-65 0.17701160718883102 1106 66-67 0.16775677878331408 1106 68-69 0.22822664904196444 1106 70-71 0.15152279549948844 1106 72-73 0.18776466221786592 1106 74-75 0.16829404156042926 1106 76-77 0.13116805739130655 1106 78-79 0.1501172519297569 1106 80-81 0.08890887475440223 1106 82-83 0.019105417167537553 1106 84-85 -0.0012711845939890054 1106 86-87 0.042433511923974265 1106 88-89 0.061092797168541324 1106 90-91 0.03614408533680802 1106 92-93 0.12189127716846215 1106 94-95 0.13897408394496935 1106 96-97 0.09038973870331901 1106 98-99 0.06172135699252834 1106 100-101 0.020178760158422904 1107 1 0.03107006857775474 1107 2 0.07726659432867677 1107 3 0.08660319417062823 1107 4 0.06188818597266277 1107 5 0.048681912485214696 1107 6 0.04677571293166949 1107 7 0.04963525967249183 1107 8 0.05617337699292335 1107 9 0.08470745029563886 1107 10-11 0.08471903623000543 1107 12-13 0.04983460842635168 1107 14-15 0.05806448237127171 1107 16-17 0.04839764139349967 1107 18-19 0.06515000507206992 1107 20-21 0.0776051596021361 1107 22-23 0.07566676641969394 1107 24-25 0.08399914856995849 1107 26-27 0.14780558567889557 1107 28-29 0.09292801339451273 1107 30-31 0.12737038531062694 1107 32-33 0.16618240247441207 1107 34-35 0.15567118195694718 1107 36-37 0.1026445310167361 1107 38-39 0.09307320122906049 1107 40-41 0.10956305606325856 1107 42-43 0.12267271013281089 1107 44-45 0.13129619511049384 1107 46-47 0.16702466216087686 1107 48-49 0.10208416042327428 1107 50-51 0.1477886162793851 1107 52-53 0.10693546469556736 1107 54-55 0.15100852008939825 1107 56-57 0.12046432020721909 1107 58-59 0.16524414361206397 1107 60-61 0.17668652271247254 1107 62-63 0.1413407778244249 1107 64-65 0.19551533730754045 1107 66-67 0.19626147280686013 1107 68-69 0.18111954693942067 1107 70-71 0.15942547508539207 1107 72-73 0.177907414757982 1107 74-75 0.18648903263239447 1107 76-77 0.046873999421571 1107 78-79 0.12366164877619212 1107 80-81 0.11440221591524136 1107 82-83 0.03863715030789194 1107 84-85 0.08815483836271909 1107 86-87 0.12172873762034442 1107 88-89 0.10021296591689577 1107 90-91 0.10667758916002867 1107 92-93 0.060936237742172494 1107 94-95 0.08674090256651468 1107 96-97 0.12126118204508884 1107 98-99 0.07934296299128718 1107 100-101 -0.06224206136119648 1108 1 0.03425279860541508 1108 2 0.05756607596818242 1108 3 0.04027361064581214 1108 4 0.051546420968215045 1108 5 0.031970410294363205 1108 6 0.06470041453079745 1108 7 0.07033529737958588 1108 8 0.04731405285578916 1108 9 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38-39 0.39484454956937753 2201 40-41 0.341224141625176 2201 42-43 0.33134935014194156 2201 44-45 0.3518464069774936 2201 46-47 0.42071813663901025 2201 48-49 0.39471133555244364 2201 50-51 0.3517375388751631 2201 52-53 0.40592419994513307 2201 54-55 0.3715932702950795 2201 56-57 0.4105298263301691 2201 58-59 0.3949946672740481 2201 60-61 0.4015503063961745 2201 62-63 0.3949463770000321 2201 64-65 0.3943919268940448 2201 66-67 0.4632390041248442 2201 68-69 0.4852338990701881 2201 70-71 0.38733209171627436 2201 72-73 0.4494223330250904 2201 74-75 0.40186443590844334 2201 76-77 0.3971880373363632 2201 78-79 0.3912599512917865 2201 80-81 0.31267588544264413 2201 82-83 0.3417733654153281 2201 84-85 0.38513892057070365 2201 86-87 0.3983936460458466 2201 88-89 0.3934316300054803 2201 90-91 0.44269104366644285 2201 92-93 0.4008051991953643 2201 94-95 0.3470792479245084 2201 96-97 0.3220348849387342 2201 98-99 0.4193174507697961 2201 100-101 0.5094227574614685 2202 1 0.28114896434890824 2202 2 0.24834982539469053 2202 3 0.24668690606819155 2202 4 0.14213023177899942 2202 5 0.21910240442820594 2202 6 0.21361972269533425 2202 7 0.24512134512879413 2202 8 0.21200126951423925 2202 9 0.2464438543800469 2202 10-11 0.23328367873470057 2202 12-13 0.23653732879452605 2202 14-15 0.31259523785774945 2202 16-17 0.370101534244732 2202 18-19 0.31897580008502757 2202 20-21 0.2924030674344422 2202 22-23 0.3112639220370639 2202 24-25 0.27749230573328276 2202 26-27 0.3204488770339893 2202 28-29 0.3170184017208584 2202 30-31 0.39927243475536045 2202 32-33 0.3527210398980003 2202 34-35 0.33151644314216355 2202 36-37 0.30213288196367216 2202 38-39 0.2590780929255345 2202 40-41 0.273225636113267 2202 42-43 0.25190475215796226 2202 44-45 0.2873659557411301 2202 46-47 0.36819660700284373 2202 48-49 0.3069209782903428 2202 50-51 0.28201128417616417 2202 52-53 0.25158467648599014 2202 54-55 0.28246557053501675 2202 56-57 0.30446707094743175 2202 58-59 0.334824565539499 2202 60-61 0.3239615558970286 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0.31794122071974584 2203 86-87 0.28794125816467897 2203 88-89 0.34559186001413167 2203 90-91 0.2868739532657685 2203 92-93 0.3132789853559821 2203 94-95 0.3386266452061726 2203 96-97 0.3196516191515606 2203 98-99 0.3417832607376923 2203 100-101 0.5039907586599952 2204 1 0.20560506982218385 2204 2 0.2041473642486764 2204 3 0.18280902697508594 2204 4 0.10747576019122107 2204 5 0.11852939510344385 2204 6 0.18709553911769916 2204 7 0.20991008629485464 2204 8 0.09121138719115152 2204 9 0.16115475769069576 2204 10-11 0.21167906232124523 2204 12-13 0.17546458647981922 2204 14-15 0.23737200963893912 2204 16-17 0.22495446992410706 2204 18-19 0.25381607758645686 2204 20-21 0.23782068464262096 2204 22-23 0.23891412563743586 2204 24-25 0.2400346556248394 2204 26-27 0.2333707802904854 2204 28-29 0.19467578153323473 2204 30-31 0.2850764620555495 2204 32-33 0.29686256006355904 2204 34-35 0.34556034204126007 2204 36-37 0.2564314804662189 2204 38-39 0.17541194245560376 2204 40-41 0.15758278703550133 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-0.18823350408971606 2214 50-51 -0.16107885772590436 2214 52-53 -0.14108486266135145 2214 54-55 -0.12201332548547583 2214 56-57 -0.11817188098218168 2214 58-59 -0.14328362405660044 2214 60-61 -0.16535477529839682 2214 62-63 -0.13656628091688816 2214 64-65 -0.2007402615244871 2214 66-67 -0.10684083341823225 2214 68-69 -0.099064094701653 2214 70-71 -0.13349121424611354 2214 72-73 -0.09212493606706573 2214 74-75 -0.12560234396789838 2214 76-77 -0.12968274382202338 2214 78-79 -0.14841500694583232 2214 80-81 -0.19866097709412855 2214 82-83 -0.1593990112283592 2214 84-85 -0.1523765417302343 2214 86-87 -0.1919725265518366 2214 88-89 -0.1414014742989167 2214 90-91 -0.17735540939099792 2214 92-93 -0.1646739883759345 2214 94-95 -0.17269332955396877 2214 96-97 -0.16917242840824187 2214 98-99 -0.19822722626474487 2214 100-101 -0.303109034444482 2215 1 -0.21713866866419806 2215 2 -0.2621015255349093 2215 3 -0.24823305592156686 2215 4 -0.16821935700935597 2215 5 -0.18759876340396886 2215 6 -0.204508219712352 2215 7 -0.22781981869012213 2215 8 -0.25966890934777354 2215 9 -0.2840929798248766 2215 10-11 -0.2578710385287266 2215 12-13 -0.26301912791893756 2215 14-15 -0.31227860623969406 2215 16-17 -0.28884668767831556 2215 18-19 -0.3101691961028763 2215 20-21 -0.33871952723733756 2215 22-23 -0.31089913399687674 2215 24-25 -0.27903074404604666 2215 26-27 -0.21993839572672158 2215 28-29 -0.3191857566602323 2215 30-31 -0.335717670174418 2215 32-33 -0.24490055090532792 2215 34-35 -0.2148523084119489 2215 36-37 -0.24361732410771708 2215 38-39 -0.287859227987866 2215 40-41 -0.338529610095037 2215 42-43 -0.24093846438125155 2215 44-45 -0.2953852638169323 2215 46-47 -0.3313203917023415 2215 48-49 -0.31283062822472374 2215 50-51 -0.30943590183333214 2215 52-53 -0.3254139967769447 2215 54-55 -0.29384246480481835 2215 56-57 -0.26658774238592997 2215 58-59 -0.31730763825941466 2215 60-61 -0.26979461946019967 2215 62-63 -0.2972067278109023 2215 64-65 -0.3331586603197394 2215 66-67 -0.19921132517974627 2215 68-69 -0.1032158896763562 2215 70-71 -0.1467658501442486 2215 72-73 -0.1869664037652896 2215 74-75 -0.20319524904255104 2215 76-77 -0.24882853970482444 2215 78-79 -0.27041466712577744 2215 80-81 -0.22341079832989408 2215 82-83 -0.2627640178943409 2215 84-85 -0.2337103908670386 2215 86-87 -0.28884870460561274 2215 88-89 -0.26274947727297615 2215 90-91 -0.30227449648258187 2215 92-93 -0.28496378708884507 2215 94-95 -0.32781253007769706 2215 96-97 -0.300966404806573 2215 98-99 -0.2374000401673051 2215 100-101 -0.3509468105005027 2216 1 -0.3197235807852721 2216 2 -0.2782043257241469 2216 3 -0.29566589132851107 2216 4 -0.19517547061273177 2216 5 -0.31588340548526617 2216 6 -0.28359605277518085 2216 7 -0.36639079733495095 2216 8 -0.30598234375979416 2216 9 -0.47998951667013046 2216 10-11 -0.5211281139387793 2216 12-13 -0.47253310566399165 2216 14-15 -0.6574290962101372 2216 16-17 -0.6073375891034019 2216 18-19 -0.5227609458891322 2216 20-21 -0.4626016663171555 2216 22-23 -0.43118684662719176 2216 24-25 -0.37596809540549714 2216 26-27 -0.2842442300365491 2216 28-29 -0.42042015523637133 2216 30-31 -0.4465553654972325 2216 32-33 -0.40791700663731234 2216 34-35 -0.5370210430012037 2216 36-37 -0.4466219419318378 2216 38-39 -0.41013377971277976 2216 40-41 -0.46348586797208924 2216 42-43 -0.3582596543696681 2216 44-45 -0.34309579803485946 2216 46-47 -0.36083906700871893 2216 48-49 -0.45299018130170765 2216 50-51 -0.45664987461814377 2216 52-53 -0.46274595795479456 2216 54-55 -0.4454560524509432 2216 56-57 -0.3713786575716753 2216 58-59 -0.3343917967439083 2216 60-61 -0.349869245748728 2216 62-63 -0.4121090417365423 2216 64-65 -0.42952886815382385 2216 66-67 -0.3548947130135076 2216 68-69 -0.33581297438819746 2216 70-71 -0.4293259023921152 2216 72-73 -0.41343593375314214 2216 74-75 -0.2661681319931404 2216 76-77 -0.27295996421662494 2216 78-79 -0.21188559584818734 2216 80-81 -0.36894349218330547 2216 82-83 -0.36460869142250374 2216 84-85 -0.38364010345575394 2216 86-87 -0.35798447066146366 2216 88-89 -0.2484771325885191 2216 90-91 -0.3185246371560737 2216 92-93 -0.24755781431897006 2216 94-95 -0.22394422500262579 2216 96-97 -0.27578900019653574 2216 98-99 -0.298292020791429 2216 100-101 -0.42480310299256274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 34.0 13 319.0 14 1561.0 15 4083.0 16 7685.0 17 13531.0 18 27455.0 19 49761.0 20 43403.0 21 37923.0 22 37089.0 23 39166.0 24 43684.0 25 54510.0 26 68395.0 27 86404.0 28 107762.0 29 133160.0 30 164792.0 31 216976.0 32 303642.0 33 452600.0 34 776358.0 35 1431237.0 36 1459960.0 37 2407892.0 38 2268163.0 39 376202.0 40 200.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.140172860923695 36.070705348107204 23.318302700936595 20.47081909003251 2 19.62457897389756 34.98948082476364 28.495809759797247 16.890130441541558 3 19.071325943687317 35.32215621893828 25.72470192529643 19.881815912077975 4 21.402427936819 33.869831683774656 25.00334936569326 19.724391013713085 5 21.751138831443527 33.80511496662411 22.67805105545061 21.765695146481754 6 22.093301739691633 32.575440811385015 23.120402866039505 22.210854582883847 7 22.21767579459108 33.60830793436939 22.656541626821305 21.517474644218222 8 22.789470461044193 32.48440966840809 23.17221204169984 21.553907828847883 9 21.20283212187734 32.244979484546285 23.98714898788858 22.5650394056878 10-11 22.01485309427687 32.5370196769346 23.26804347109229 22.180083757696238 12-13 21.55896720824952 32.66386689215608 23.595098902858975 22.18206699673543 14-15 21.234761799330126 32.81748547901582 23.942161023935483 22.005591697718568 16-17 21.329768841948567 33.12855722892985 23.834910660027607 21.706763269093976 18-19 20.767061273135827 33.1515882400049 24.54841976832376 21.53293071853551 20-21 20.134342068692618 33.26034605401382 25.074062907777027 21.531248969516533 22-23 19.935702542408812 32.85550619703315 25.80580745151428 21.402983809043757 24-25 19.8347599150175 32.369245191469716 27.04820542776252 20.747789465750262 26-27 19.86144178180602 32.36009685366899 27.38672219107872 20.391739173446265 28-29 20.00665633435243 32.4952256228831 27.168165480334842 20.329952562429632 30-31 19.975202445837787 32.33767824419749 27.334715162592627 20.35240414737209 32-33 19.969987610644484 32.3977077336901 27.335577235619162 20.29672742004626 34-35 20.029536600417373 32.49878697374681 27.154494792230977 20.317181633604832 36-37 20.011612076559622 32.41858120680802 27.13576472472548 20.434041991906877 38-39 19.997931966892626 32.51763010000989 26.979965046000782 20.504472887096696 40-41 20.18646215593629 32.75222231120365 26.486816877788193 20.574498655071864 42-43 20.17859043993989 32.6838735814659 26.48952557718851 20.6480104014057 44-45 20.160444509348547 32.95405103660748 26.285440387414678 20.600064066629294 46-47 20.466584070963215 33.08820938481903 25.676529473004866 20.768677071212885 48-49 20.359993216474546 33.07634292605486 25.595372128189787 20.968291729280804 50-51 20.339001973817474 33.138223752702814 25.557535130653523 20.965239142826185 52-53 20.463809420620976 33.20270493077506 25.30538583656414 21.028099812039816 54-55 20.42187875390406 33.10731160406823 25.396713758779722 21.07409588324799 56-57 20.38463060406352 33.10312371925626 25.56318806853245 20.94905760814777 58-59 20.544142378662045 33.23573222033268 25.251551024830533 20.96857437617475 60-61 20.442728673114157 33.17839258711413 25.299817692753408 21.07906104701831 62-63 20.430772709500232 33.264529228044225 25.38512994690949 20.91956811554605 64-65 20.46960839272844 33.318015441941974 25.178467959619184 21.033908205710407 66-67 20.480490298145366 33.14092774132156 25.311368529152674 21.0672134313804 68-69 20.48349106600276 33.252045656894936 25.265697501872538 20.99876577522977 70-71 20.58782074533986 33.30914504025363 25.127341847285884 20.97569236712063 72-73 20.50523047597369 33.24311556830691 25.224091921309583 21.027562034409815 74-75 20.457073002981925 33.38011767532351 25.202436416225815 20.960372905468745 76-77 20.562161118151113 33.42741392224384 25.037116247956696 20.97330871164835 78-79 20.487716637067255 33.33644401942726 25.17365825164053 21.00218109186495 80-81 20.35120597800697 33.380193835904606 25.330364423240127 20.938235762848294 82-83 20.435968958300908 33.466195873091124 25.09400451410101 21.00383065450696 84-85 20.37386175740417 33.32371077685499 25.246053542743276 21.056373922997565 86-87 20.344471191215334 33.43065493996109 25.29699122381394 20.927882645009632 88-89 20.37205752806448 33.55333782427843 25.00764088769968 21.066963759957414 90-91 20.28747544505109 33.40089222202855 25.139674673425066 21.171957659495284 92-93 20.31861842198239 33.479298470409226 25.211792028415438 20.99029107919295 94-95 20.39389671140339 33.52089467163497 25.03564177332661 21.049566843635027 96-97 20.235609740011963 33.535144785871424 25.079913698481715 21.1493317756349 98-99 20.268371815282872 33.52384619631589 25.218595550323002 20.98918643807824 100-101 20.224699569905642 33.602231026149546 24.89437956651388 21.278689837430928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 849086.0 1 456105.5 2 41858.0 3 16337.0 4 10774.0 5 8582.5 6 7365.5 7 6748.5 8 6288.5 9 6000.0 10 5842.5 11 5818.5 12 6306.0 13 8130.5 14 9701.5 15 11055.0 16 39884.0 17 39612.0 18 9520.0 19 7377.0 20 8038.5 21 10015.5 22 12980.0 23 16962.5 24 22013.0 25 28019.5 26 35708.5 27 45329.5 28 56647.5 29 68610.5 30 80844.5 31 95402.5 32 115604.0 33 137363.0 34 156609.5 35 178529.5 36 202171.0 37 231595.0 38 261078.5 39 286028.5 40 310608.5 41 336246.0 42 367397.5 43 400042.0 44 418821.5 45 422654.5 46 423956.5 47 419996.0 48 415756.5 49 411779.0 50 399827.0 51 377599.0 52 338840.0 53 299435.5 54 269906.0 55 245471.5 56 222558.5 57 198665.5 58 180014.5 59 163267.5 60 143638.5 61 124916.5 62 108683.0 63 93854.0 64 80457.5 65 66787.5 66 54606.5 67 42725.0 68 31690.0 69 23050.0 70 16031.0 71 10658.5 72 6953.0 73 4459.0 74 2603.0 75 1329.5 76 640.5 77 326.5 78 181.5 79 74.5 80 14.5 81 5.0 82 3.5 83 3.0 84 1.0 85 1.0 86 1.0 87 0.5 88 1.5 89 1.0 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16989904795104555 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.8843126263078307E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001681749018979739 82-83 9.23313186890837E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.276075353661926E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.061395E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.581275907666175 #Duplication Level Percentage of deduplicated Percentage of total 1 78.05505644754746 28.553535558961908 2 11.50030471104487 8.41391639313931 3 3.543965849705044 3.8892837766622037 4 1.5472021217389091 2.263945108010302 5 0.8693221721309338 1.590045711568668 6 0.5511444973445488 1.2096941353411759 7 0.41093604855465016 1.0522795480808633 8 0.3145431280650435 0.9205111162086187 9 0.25185882342766586 0.829198539862886 >10 2.3928163915745997 18.869416080652687 >50 0.3618078926512915 9.14754020173838 >100 0.1980546396703206 12.140664009128471 >500 0.001957734837553617 0.43274498610949036 >1k 8.493719598610596E-4 0.5866789629783165 >5k 5.147707067103124E-5 0.14610949042125754 >10k+ 1.286926766775781E-4 9.954436381135503 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 949883 8.949382652075807 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32060 0.3020553139971453 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31857 0.3001427366814428 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 31615 0.29786271840361034 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11826 0.11141940559358204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.087644091031143E-4 0.0 0.0 0.0 0.0 2 5.087644091031143E-4 0.0 0.0 0.0 0.0 3 5.181859722346535E-4 0.0 0.0 0.0 0.0 4 5.181859722346535E-4 0.0 0.0 0.0 0.0 5 5.181859722346535E-4 0.0 0.0 0.0 0.0 6 5.276075353661926E-4 9.421563131539153E-6 0.0 0.0 0.0 7 5.464506616292709E-4 9.421563131539153E-6 0.0 0.0 0.0 8 7.34881924260054E-4 9.421563131539153E-6 0.0 0.0 0.0 9 7.914113030492889E-4 1.8843126263078307E-5 0.0 0.0 0.0 10-11 8.24386774009676E-4 2.3553907828847886E-5 0.0 0.0 0.0 12-13 8.997592790619892E-4 4.710781565769577E-5 0.0 0.0 0.0 14-15 9.704210025485328E-4 4.710781565769577E-5 0.0 0.0 0.0 16-17 0.001078768978561233 4.710781565769577E-5 0.0 0.0 0.0 18-19 0.0012059600808370116 4.710781565769577E-5 0.0 0.0 0.0 20-21 0.0013095972752839424 5.652937878923492E-5 0.0 0.0 0.0 22-23 0.0014603422853885688 6.595094192077407E-5 0.0 0.0 0.0 24-25 0.0016487735480193518 6.595094192077407E-5 0.0 0.0 0.0 26-27 0.0017900969949924393 7.066172348654364E-5 0.0 0.0 0.0 28-29 0.001950263568228605 8.479406818385238E-5 0.0 0.0 0.0 30-31 0.0021010085783332313 8.479406818385238E-5 0.0 0.0 0.0 32-33 0.0023082829672270927 1.036371944469307E-4 0.0 0.0 0.0 34-35 0.002473160322029028 1.1305875757846985E-4 0.0 0.0 0.0 36-37 0.0026333268952651936 1.1305875757846985E-4 0.0 0.0 0.0 38-39 0.0027982042500671288 1.1305875757846985E-4 0.0 0.0 0.0 40-41 0.003024321765224068 1.1305875757846985E-4 0.0 0.0 0.0 42-43 0.003137380522802538 1.22480320710009E-4 0.0 0.0 0.0 44-45 0.0032692824066440865 1.3190188384154815E-4 0.0 0.0 0.0 46-47 0.0034624244508406387 1.5074501010462645E-4 0.0 0.0 0.0 48-49 0.0035472185190244913 1.5074501010462645E-4 0.0 0.0 0.0 50-51 0.003735649781655274 1.5074501010462645E-4 0.0 0.0 0.0 52-53 0.00477673250769035 1.6016657323616562E-4 0.0 0.0 0.0 54-55 0.006105172909237372 1.648773548019352E-4 0.0 0.0 0.0 56-57 0.007626755354980945 1.7900969949924392E-4 0.0 0.0 0.0 58-59 0.009327347500223761 1.7900969949924392E-4 0.0 0.0 0.0 60-61 0.011235214034360442 1.7900969949924392E-4 0.0 0.0 0.0 62-63 0.013406884336180216 1.7900969949924392E-4 0.0 0.0 0.0 64-65 0.016285171872865427 1.8372048106501352E-4 0.0 0.0 0.0 66-67 0.019563875842641056 1.884312626307831E-4 0.0 0.0 0.0 68-69 0.023973167388201377 2.072743888938614E-4 0.0 0.0 0.0 70-71 0.02884411552720712 2.072743888938614E-4 0.0 0.0 0.0 72-73 0.03461482294527485 2.072743888938614E-4 0.0 0.0 0.0 74-75 0.04147843168660112 2.1669595202540056E-4 0.0 0.0 0.0 76-77 0.050419495098431785 2.5438220455155716E-4 0.0 0.0 0.0 78-79 0.06112239081586027 2.7322533081463544E-4 0.0 0.0 0.0 80-81 0.07385563338813542 2.8264689394617463E-4 0.0 0.0 0.0 82-83 0.08934939395795155 2.873576755119442E-4 0.0 0.0 0.0 84-85 0.10714201593186326 3.014900202092529E-4 0.0 0.0 4.710781565769577E-6 86-87 0.1284253270460102 3.297547096038704E-4 0.0 0.0 9.421563131539153E-6 88-89 0.1536185868597459 3.533086174327183E-4 0.0 0.0 9.421563131539153E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6715 0.0 88.20983 1 TCGACGG 7490 0.0 79.39766 2 TATCTCG 11125 0.0 54.09559 2 ATATCTC 11785 0.0 51.95683 1 CGACGGC 11560 0.0 51.443645 3 GACGGCG 11860 0.0 50.10232 4 ACGGCGC 12200 0.0 48.628162 5 ATCTCGA 19390 0.0 46.447945 1 TCTCGAG 21370 0.0 41.92011 2 CGGCGCG 15140 0.0 39.404785 6 CTCGAGG 26035 0.0 34.68245 3 TCGAGGG 26585 0.0 34.07213 4 AGGGCGC 28880 0.0 31.364529 7 GGGCGCG 29330 0.0 30.850924 8 CGAGGGC 29805 0.0 30.486746 5 GCGTAAT 605 0.0 29.049025 28-29 GAGGGCG 32305 0.0 28.142162 6 TGCGTAA 780 0.0 22.836098 26-27 CGCGCCG 44150 0.0 22.141134 9 GGCGCGC 45030 0.0 21.750635 7 >>END_MODULE