FastQCFastQC Report
Thu 26 May 2016
SRR1051297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7766403
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT4365035.620401104604023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA574230.7393770320700587No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT387430.4988538452099382No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA343380.4421351814990801No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA286540.36894814755299205No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC280780.3615315867590183No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT255690.32922576899499034No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC228710.29448639222043976No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC184050.236982294119942No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG168360.21677989154052396No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA144620.1862123302125836No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134010.17255092222229518No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG131530.16935768076933427No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT117300.15103517033561098No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC97590.1256566263687321No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT92630.11927014346281026No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT84290.10853158147986913No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTACC83150.10706372048939515No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG83050.10693496075338868No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTATT79450.10229961025715509No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTACT78130.10059998174186945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2577000.094.578521
TCGACGG2608300.093.4399262
CGACGGC2630900.092.604763
ATATCTC1096150.092.3381351
GACGGCG2645200.092.114914
ACGGCGC2648000.092.0013665
CGGCGCG2662700.091.463136
TATCTCG1112750.090.717322
ATCTCGA1146100.088.019553
TCTCGAG1140550.086.827834
CTCGAGG1159400.085.506275
TCGAGGG1179700.084.1154256
AGGGCGC1201950.083.068119
CGAGGGC1202100.082.5717247
GAGGGCG1213350.082.146718
CTCGACG24150.073.560135
TCTCGAC27850.063.7873274
GGCGCGC3867450.063.3791927
CGCGCCG3866850.063.346039
GCGCGCC3868600.063.3308838
Produced by FastQC (version 0.11.5)