##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051297_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7766403
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923333620467545	31.0	31.0	34.0	30.0	34.0
2	32.290852019911924	33.0	31.0	34.0	31.0	34.0
3	32.338316592636254	34.0	31.0	34.0	31.0	34.0
4	36.053108884511914	37.0	37.0	37.0	35.0	37.0
5	35.9335365419487	37.0	35.0	37.0	35.0	37.0
6	35.97260482104779	37.0	36.0	37.0	35.0	37.0
7	35.91207770186533	37.0	35.0	37.0	35.0	37.0
8	36.03447271536128	37.0	36.0	37.0	35.0	37.0
9	37.65923607106147	39.0	38.0	39.0	35.0	39.0
10-11	37.6647108706566	39.0	38.0	39.0	35.0	39.0
12-13	37.69502535472341	39.0	38.0	39.0	35.0	39.0
14-15	39.09191449374955	41.0	39.0	41.0	36.0	41.0
16-17	38.85784667625411	40.5	38.5	41.0	34.5	41.0
18-19	38.77090732994412	40.5	38.5	41.0	34.5	41.0
20-21	38.961216408677224	40.5	39.0	41.0	35.0	41.0
22-23	38.915836005419756	40.5	38.5	41.0	35.0	41.0
24-25	38.931776847016565	41.0	39.0	41.0	35.0	41.0
26-27	39.06805834052135	41.0	39.0	41.0	35.0	41.0
28-29	38.625990114084985	40.0	38.5	41.0	35.0	41.0
30-31	37.21881197769417	37.5	36.0	40.5	34.5	41.0
32-33	36.501967126866845	36.0	35.0	40.0	34.0	41.0
34-35	36.159750466206816	35.0	35.0	40.0	33.0	41.0
36-37	36.05401631875142	35.0	35.0	40.0	33.0	41.0
38-39	35.414489886759675	35.0	35.0	40.0	31.0	41.0
40-41	32.29826523295276	35.0	29.5	40.0	17.5	41.0
42-43	32.47032043019143	35.0	30.0	40.0	18.0	41.0
44-45	32.550553969450206	35.0	30.0	40.0	18.0	41.0
46-47	32.879034992132134	35.0	31.0	40.0	20.0	41.0
48-49	32.274106494344935	35.0	29.5	40.0	18.0	41.0
50-51	32.391371076159714	35.0	29.5	40.0	18.0	41.0
52-53	32.27782301536503	35.0	30.0	40.0	18.0	41.0
54-55	31.758571374676283	35.0	29.0	39.0	17.5	41.0
56-57	31.31969986620576	35.0	29.0	39.0	13.5	41.0
58-59	30.89777732368511	35.0	29.0	39.0	9.0	41.0
60-61	30.53280392480277	34.5	29.0	38.5	7.0	41.0
62-63	30.11738922123923	34.0	28.5	38.0	7.0	40.5
64-65	29.66276943135709	34.0	27.0	37.0	2.0	40.0
66-67	29.196385315055117	34.0	26.0	37.0	2.0	40.0
68-69	28.780123951847465	34.0	25.0	36.0	2.0	40.0
70-71	28.353901928601953	34.0	25.0	36.0	2.0	39.0
72-73	27.968202396913988	34.0	25.0	35.0	2.0	39.0
74-75	27.619557148914367	34.0	25.0	35.0	2.0	38.5
76-77	27.307791908300405	33.5	25.0	35.0	2.0	37.0
78-79	27.07856597191776	33.0	24.5	35.0	2.0	37.0
80-81	26.878763824128107	33.0	24.0	35.0	2.0	36.0
82-83	26.71889380450641	33.0	24.0	35.0	2.0	36.0
84-85	26.532010507309497	33.0	24.0	35.0	2.0	36.0
86-87	26.320177822345812	33.0	23.5	35.0	2.0	35.0
88-89	26.091462032037224	33.0	23.0	35.0	2.0	35.0
90-91	25.847529158607916	33.0	21.5	35.0	2.0	35.0
92-93	25.53523271970306	33.0	19.0	35.0	2.0	35.0
94-95	25.209022568105212	33.0	18.5	35.0	2.0	35.0
96-97	24.832608222880012	33.0	11.0	35.0	2.0	35.0
98-99	24.35395838974619	33.0	2.0	35.0	2.0	35.0
100-101	22.563872361503776	31.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1781247975753324
1101	2	0.16317899259879454
1101	3	0.1731025740515122
1101	4	0.152108424165057
1101	5	0.18900091064041646
1101	6	0.20390355085230993
1101	7	0.19459860693228137
1101	8	0.1774945089775244
1101	9	0.2428971668906641
1101	10-11	0.22436552135032883
1101	12-13	0.2062912308968592
1101	14-15	0.28844209732202586
1101	16-17	0.3124946680837155
1101	18-19	0.3058871750293264
1101	20-21	0.31991366467815396
1101	22-23	0.30448101550221907
1101	24-25	0.31869573183405464
1101	26-27	0.24493691294743059
1101	28-29	0.22493278773794145
1101	30-31	0.19667400804980417
1101	32-33	0.18497069062634353
1101	34-35	0.23398177576300583
1101	36-37	0.22266203999756584
1101	38-39	0.16260083769632416
1101	40-41	-0.49006921286538585
1101	42-43	-0.375139150803129
1101	44-45	-0.27911850343690503
1101	46-47	-0.22630875436190934
1101	48-49	-0.10274010480750206
1101	50-51	0.017519042641055194
1101	52-53	-0.11794122939033969
1101	54-55	-0.2835742563816517
1101	56-57	-0.3241150904307162
1101	58-59	-0.39580942263949837
1101	60-61	-0.3973510680610417
1101	62-63	-0.3772661907471111
1101	64-65	-0.43603362708173776
1101	66-67	-0.48827615344630004
1101	68-69	-0.6211429268347821
1101	70-71	-0.6487303261952952
1101	72-73	-0.6225308022045617
1101	74-75	-0.6718041400074739
1101	76-77	-0.7054647469388122
1101	78-79	-0.6932877822709926
1101	80-81	-0.7358864058811889
1101	82-83	-0.7920709677394235
1101	84-85	-0.8003703654237562
1101	86-87	-0.7956499538104644
1101	88-89	-0.7886259246412877
1101	90-91	-0.77572213009244
1101	92-93	-0.8039614053787965
1101	94-95	-0.778160261877094
1101	96-97	-0.799278079292197
1101	98-99	-0.7923763586788155
1101	100-101	-0.6438551812109523
1102	1	0.10724121507359641
1102	2	0.18571391788589864
1102	3	0.1800538008973973
1102	4	0.1613392485782299
1102	5	0.16548439702444284
1102	6	0.1806278802027066
1102	7	0.17048847489772356
1102	8	0.1098422793858731
1102	9	0.1857488761624282
1102	10-11	0.20660695432944465
1102	12-13	0.19672924152612126
1102	14-15	0.2807682240008802
1102	16-17	0.2864667437816806
1102	18-19	0.34673195050687156
1102	20-21	0.30796834423050257
1102	22-23	0.298623497592871
1102	24-25	0.26585923252537214
1102	26-27	0.2411568713812855
1102	28-29	0.24213979724492418
1102	30-31	0.22524931349850874
1102	32-33	0.25135337224401155
1102	34-35	0.2648034752768922
1102	36-37	0.24764401479138343
1102	38-39	0.18461726788027022
1102	40-41	-0.28530569234602865
1102	42-43	-0.2582921860892
1102	44-45	-0.28797259038042
1102	46-47	-0.28226194632838286
1102	48-49	-0.18918471707055318
1102	50-51	-0.1452952548433899
1102	52-53	-0.18434343648575435
1102	54-55	-0.20267212851687688
1102	56-57	-0.37488105822689377
1102	58-59	-0.4406556277174971
1102	60-61	-0.5839190615906524
1102	62-63	-0.5586422178687585
1102	64-65	-0.5890861811709946
1102	66-67	-0.568706871533017
1102	68-69	-0.5593692926352922
1102	70-71	-0.5913646528599408
1102	72-73	-0.6764451690244364
1102	74-75	-0.6833244148685118
1102	76-77	-0.7196806037525185
1102	78-79	-0.6924350573921956
1102	80-81	-0.6331514860872396
1102	82-83	-0.6536334257843386
1102	84-85	-0.6313091927212113
1102	86-87	-0.7596891266061938
1102	88-89	-0.7159617769049547
1102	90-91	-0.6867037055663197
1102	92-93	-0.679879455561295
1102	94-95	-0.6041421574716139
1102	96-97	-0.6403282587196593
1102	98-99	-0.6402301049964052
1102	100-101	-0.5945882386615366
1103	1	0.06805211498264185
1103	2	0.1179793664508253
1103	3	0.11779326213293473
1103	4	0.09469730508767782
1103	5	0.11521377530829113
1103	6	0.1630112634162444
1103	7	0.1815464355609251
1103	8	0.12932263476878347
1103	9	0.2065940731051512
1103	10-11	0.1700950081300192
1103	12-13	0.16537351210502038
1103	14-15	0.19437662079838702
1103	16-17	0.24730910580865384
1103	18-19	0.28541149095917007
1103	20-21	0.21723417705445058
1103	22-23	0.20965470833053246
1103	24-25	0.20729051283120725
1103	26-27	0.2099694650828141
1103	28-29	0.18156644028476165
1103	30-31	0.17595211736065863
1103	32-33	0.20513664521504893
1103	34-35	0.20466475314620425
1103	36-37	0.17261413132123238
1103	38-39	0.2079463887416111
1103	40-41	-0.20853466486830996
1103	42-43	-0.26183657497326607
1103	44-45	-0.25902084703073314
1103	46-47	-0.1347122293734344
1103	48-49	-0.08653322372675376
1103	50-51	0.016600293268510313
1103	52-53	-0.014821190725790245
1103	54-55	-0.2216300259011632
1103	56-57	-0.23994962981000256
1103	58-59	-0.28444540465408963
1103	60-61	-0.3379215433453169
1103	62-63	-0.4223204472675306
1103	64-65	-0.4524131970630556
1103	66-67	-0.4667362393221026
1103	68-69	-0.5548417165243578
1103	70-71	-0.5696956693653448
1103	72-73	-0.6323839533383371
1103	74-75	-0.674034547646567
1103	76-77	-0.6567913530677458
1103	78-79	-0.679681451662475
1103	80-81	-0.6707127263793495
1103	82-83	-0.6104179029612098
1103	84-85	-0.6854948213704262
1103	86-87	-0.6554666570360936
1103	88-89	-0.673441761646064
1103	90-91	-0.6705588097035573
1103	92-93	-0.6620380041781679
1103	94-95	-0.7002351451149664
1103	96-97	-0.6469683569182045
1103	98-99	-0.5939116129790456
1103	100-101	-0.4832521715599185
1104	1	0.04223162607146591
1104	2	0.12219934744300076
1104	3	0.05214730031119075
1104	4	0.10630055636517
1104	5	0.11866872792254668
1104	6	0.10743571867084256
1104	7	0.1079394632641737
1104	8	0.10059936831127914
1104	9	0.16348348204964225
1104	10-11	0.14269759307313024
1104	12-13	0.13287448231717036
1104	14-15	0.16944587702882785
1104	16-17	0.13339998154727084
1104	18-19	0.2161900259274674
1104	20-21	0.19470439274076057
1104	22-23	0.17668215567648815
1104	24-25	0.18822961570121777
1104	26-27	0.1416014332512745
1104	28-29	0.13865789259577355
1104	30-31	0.1554000634903403
1104	32-33	0.20183325274233255
1104	34-35	0.22912221076364858
1104	36-37	0.23307512349035875
1104	38-39	0.188501856975968
1104	40-41	-0.2135859226218031
1104	42-43	-0.1426601992414689
1104	44-45	-0.045319532491113534
1104	46-47	-0.022586351258851778
1104	48-49	0.0671311640460246
1104	50-51	0.16051931975371758
1104	52-53	0.006952181602876806
1104	54-55	0.02745827767057918
1104	56-57	0.05547843953220166
1104	58-59	0.03035550364628037
1104	60-61	-0.040267083298363815
1104	62-63	-0.052602138297441314
1104	64-65	-0.1570664942441482
1104	66-67	-0.18184657081272348
1104	68-69	-0.2285935515319828
1104	70-71	-0.2075897225771577
1104	72-73	-0.22877579305246698
1104	74-75	-0.2920635228399391
1104	76-77	-0.31351332940188925
1104	78-79	-0.39418110814298757
1104	80-81	-0.37184029343319125
1104	82-83	-0.3630837139222187
1104	84-85	-0.22451794398151037
1104	86-87	-0.20360134684847608
1104	88-89	-0.24699618341477247
1104	90-91	-0.2974415073301522
1104	92-93	-0.20607933925574073
1104	94-95	-0.1957101027841759
1104	96-97	-0.2387286487418585
1104	98-99	-0.22421484499117383
1104	100-101	-0.1054218758680392
1105	1	-0.03500932943984836
1105	2	0.08505243910495608
1105	3	0.04143996087608315
1105	4	0.02919538170605307
1105	5	0.027961849641549463
1105	6	0.09076873043546385
1105	7	0.05756681018835508
1105	8	0.09813781615195438
1105	9	0.13019395426038471
1105	10-11	0.09763291290639842
1105	12-13	0.08436035173799183
1105	14-15	0.1490889377562823
1105	16-17	0.14882170774941272
1105	18-19	0.20704787226956256
1105	20-21	0.18965361079420973
1105	22-23	0.18351400712083432
1105	24-25	0.16003134373568884
1105	26-27	0.19270291950704177
1105	28-29	0.20059633915222008
1105	30-31	0.19225597774725856
1105	32-33	0.17366294266120264
1105	34-35	0.17758929118069489
1105	36-37	0.18101399273556495
1105	38-39	0.1551714635897028
1105	40-41	-0.2375666660427811
1105	42-43	-0.23916422019193817
1105	44-45	-0.2688950179195615
1105	46-47	-0.1121896471988606
1105	48-49	-0.018285257347692152
1105	50-51	-0.046382437553745604
1105	52-53	-0.10973694418043323
1105	54-55	-0.10208425929517517
1105	56-57	-0.03738069736549576
1105	58-59	0.0074515635222134335
1105	60-61	-0.14853993918368147
1105	62-63	-0.18209922039934057
1105	64-65	-0.1763920121162208
1105	66-67	-0.2246322325882275
1105	68-69	-0.30251296852258847
1105	70-71	-0.27711069789997467
1105	72-73	-0.36936350093895953
1105	74-75	-0.36642226132014954
1105	76-77	-0.34658423527201165
1105	78-79	-0.278148431856458
1105	80-81	-0.3198149205558387
1105	82-83	-0.2651842063770502
1105	84-85	-0.23609444447129846
1105	86-87	-0.29093257166396214
1105	88-89	-0.2098403500088324
1105	90-91	-0.25030423745096897
1105	92-93	-0.1795920688604049
1105	94-95	-0.16457698123353737
1105	96-97	-0.15460839375958102
1105	98-99	-0.206885416249591
1105	100-101	-0.2124139345853422
1106	1	-0.008918164529855943
1106	2	-0.006275053352474913
1106	3	0.038403769684641986
1106	4	0.019047510704552906
1106	5	0.04490432742654349
1106	6	0.047332418082149275
1106	7	0.04566523852379589
1106	8	0.03336058345421122
1106	9	0.060334318146992416
1106	10-11	0.08618125374331242
1106	12-13	0.044844142516957675
1106	14-15	0.07747523624281882
1106	16-17	0.0879444386847581
1106	18-19	0.10570392693926323
1106	20-21	0.05835094211202829
1106	22-23	0.1328319271043341
1106	24-25	0.07770905011174989
1106	26-27	0.07530577786299375
1106	28-29	0.058073175607191274
1106	30-31	0.10165284547004916
1106	32-33	0.08373104680680399
1106	34-35	0.09934036167629756
1106	36-37	0.14032424406703825
1106	38-39	0.13506040259921548
1106	40-41	-0.14736712090667936
1106	42-43	-0.10575965484873251
1106	44-45	-0.07667001823128317
1106	46-47	-0.022584236089940646
1106	48-49	-0.036820112992522525
1106	50-51	0.042949063581502855
1106	52-53	0.06729215285361789
1106	54-55	0.11279438683594023
1106	56-57	-6.170192275440911E-4
1106	58-59	-0.1296199022652864
1106	60-61	-0.04424856160117585
1106	62-63	-0.058737102021229504
1106	64-65	-0.05755363286069226
1106	66-67	-0.09760719955170671
1106	68-69	-0.07335940324689716
1106	70-71	-0.08398508733209908
1106	72-73	-0.05631790043512197
1106	74-75	-0.15324319940839715
1106	76-77	-0.19072043207212985
1106	78-79	-0.19193994060140085
1106	80-81	-0.22264023509713482
1106	82-83	-0.24180730900572556
1106	84-85	-0.20219742625877046
1106	86-87	-0.2733382298795739
1106	88-89	-0.32365119808765286
1106	90-91	-0.1923041455222041
1106	92-93	-0.17613903966990918
1106	94-95	-0.23614833565215676
1106	96-97	-0.1652951580521318
1106	98-99	-0.2045941045152766
1106	100-101	-0.16878872230276087
1107	1	0.015818723361864073
1107	2	-0.042286705206677766
1107	3	-0.017309703261780385
1107	4	-0.025863701589862842
1107	5	0.023214530878377104
1107	6	0.04952309855288206
1107	7	0.033416780842806304
1107	8	0.05705334915003135
1107	9	0.02064561216400307
1107	10-11	0.06336947211910626
1107	12-13	0.05159662981320423
1107	14-15	-0.004703847294258878
1107	16-17	0.026828784898896174
1107	18-19	0.029251754105033
1107	20-21	0.030738130449847745
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2216	14-15	-0.530921707278516
2216	16-17	-0.5123507092182678
2216	18-19	-0.5031086405807557
2216	20-21	-0.46409532012468446
2216	22-23	-0.44359595946731645
2216	24-25	-0.4516112960175178
2216	26-27	-0.3638552875693293
2216	28-29	-0.3763316335083289
2216	30-31	-0.3510138126609803
2216	32-33	-0.6184825035025483
2216	34-35	-0.6230183709201214
2216	36-37	-0.72803529874151
2216	38-39	-0.5767439130888121
2216	40-41	0.6340518588347521
2216	42-43	0.5211768886048205
2216	44-45	0.42056447304051403
2216	46-47	0.1373532279775347
2216	48-49	-0.39250654652160577
2216	50-51	-0.7373775561323903
2216	52-53	-0.6678594350958988
2216	54-55	-0.4904581982001126
2216	56-57	-0.5699236027570578
2216	58-59	-0.34117661133050703
2216	60-61	-0.4012902192408383
2216	62-63	-0.31218150315103443
2216	64-65	-0.2343631749448356
2216	66-67	-0.17427860462284173
2216	68-69	-0.104610348765803
2216	70-71	-0.05063257688183498
2216	72-73	-0.007433879482814376
2216	74-75	0.025323505679892833
2216	76-77	0.1339443333925736
2216	78-79	0.09125042072692935
2216	80-81	0.12329268868261067
2216	82-83	0.02360429818716625
2216	84-85	-0.036466782596107805
2216	86-87	-3.957625612116544E-6
2216	88-89	0.06984669231717433
2216	90-91	0.07177674383779475
2216	92-93	0.06810626974206357
2216	94-95	0.04795714758816416
2216	96-97	0.007698733011284986
2216	98-99	-0.026825364035744315
2216	100-101	-0.17829285091422165
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	20.0
11	482.0
12	2018.0
13	4295.0
14	7125.0
15	10855.0
16	17370.0
17	33185.0
18	84125.0
19	279345.0
20	299874.0
21	194545.0
22	159495.0
23	140585.0
24	135717.0
25	145082.0
26	172254.0
27	210928.0
28	251207.0
29	280708.0
30	307568.0
31	344537.0
32	394184.0
33	447367.0
34	501216.0
35	598561.0
36	720211.0
37	982251.0
38	900616.0
39	140642.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.79340049698683	31.882816279299437	13.314400501750939	10.009382721962792
2	11.940495490640906	17.313961173531688	61.721378609891865	9.024164725935545
3	12.263231768941168	29.68371587207102	14.569215633028573	43.483836725959236
4	46.112234969006884	15.079232947350274	27.329408479060387	11.479123604582457
5	13.156927344615005	48.00103728843327	13.399278919726418	25.442756447225314
6	13.23008862661389	14.479508724952852	26.007921556478593	46.28248109195467
7	46.66403481766269	14.594980456203471	13.1965080874634	25.54447663867044
8	59.575211330135716	14.869689352973314	13.15229199411877	12.402807322772203
9	13.264969639098048	27.415754758026335	13.500213676781902	45.81906192609371
10-11	43.19519602575349	14.245185319381443	13.342998039117981	29.216620615747086
12-13	36.0949721512005	14.324803902141056	13.639711974771332	35.94051197188711
14-15	35.74681870101256	14.480197589540486	13.863245314465397	35.90973839498157
16-17	35.46187340523019	31.112105565472202	13.83567785498641	19.590343174311197
18-19	19.210946174181277	14.802026111701904	30.630672912543943	35.35635480157288
20-21	18.328311059830398	38.06976022233201	14.97679170138351	28.625137016454076
22-23	11.481653733394984	30.833733454212975	38.45661241117671	19.228000401215336
24-25	11.474410998244618	14.78963942509808	55.298791473993816	18.437158102663485
26-27	11.478698697453634	14.706698841149498	62.252145555671014	11.562456905725856
28-29	11.552960875195376	14.2205278299362	62.757096174380855	11.469415120487566
30-31	11.48734491372647	14.123037395818889	62.85577763605623	11.533840054398413
32-33	11.47542176217227	14.140033680971746	62.859968765463236	11.524575791392747
34-35	11.545093655325381	14.220122236767779	62.732792774209635	11.501991333697207
36-37	11.62248469465208	14.173015487349808	62.559739689016915	11.644760128981204
38-39	12.559301905914488	14.741869563039673	59.54031100369116	13.158517527354686
40-41	18.237664205681835	19.167476887305487	43.31804568987728	19.2768132171354
42-43	18.292509415233795	20.44388502631141	44.90884647629025	16.35475908216455
44-45	19.804251723738776	23.126323730560983	40.740623426314606	16.32880111938564
46-47	18.69066670889986	25.66888429559991	39.15948605808893	16.48096293741131
48-49	18.611157571915854	27.80343744716827	36.16456035052521	17.420844630390672
50-51	19.039792295094653	27.65622000300525	34.94028702862831	18.363700673271783
52-53	19.065808199754763	27.11860303927056	35.1126448112466	18.70294394972808
54-55	19.11338492220916	25.853377683336802	35.77367798194351	19.259559412510526
56-57	18.953729287547915	25.620689526412676	36.34546134162752	19.080119844411886
58-59	19.142800083899843	25.70627612293619	36.248743465926246	18.90218032723772
60-61	19.77773365610824	25.961168896334634	35.19908122202775	19.062016225529373
62-63	20.246515664973863	26.299092385496863	34.07197643490816	19.38241551462112
64-65	20.507466583951413	26.52267079109853	32.95253285208094	20.017329772869115
66-67	20.697638276046195	26.766812126540433	32.12272785741353	20.412821739999842
68-69	20.763724210551526	27.14558464195072	31.57569855697676	20.514992590520993
70-71	21.167772262139888	27.44541456321543	31.081898531405077	20.304914643239606
72-73	21.227040368623673	27.748463477880303	30.75611708534826	20.268379068147766
74-75	21.446922752542243	27.90290712085266	30.35525396666999	20.294916159935102
76-77	21.57180523917482	28.11329265203948	29.8590318614338	20.4558702473519
78-79	21.5164013982341	28.01643999627347	29.793327619063685	20.673830986428747
80-81	21.487666465660762	27.890007147878155	29.82529097246554	20.79703541399555
82-83	21.53588125718087	27.706994723482765	29.88589671499815	20.87122730433822
84-85	21.535929913202693	27.320736714835885	30.290663463110825	20.852669908850597
86-87	21.36210012137063	27.34741306471272	30.448712088416947	20.841774725499704
88-89	21.394894369699582	27.33706110180918	30.20986740354185	21.05817712494939
90-91	21.60976318877338	27.278923547173804	29.892973537149853	21.21833972690296
92-93	21.745554987438844	27.321670616801786	29.77173081843186	21.161043577327515
94-95	21.60243294096379	27.421310740635015	29.727384736537623	21.248871581863575
96-97	21.548579181379075	27.474758907051307	29.44176989012803	21.534892021441586
98-99	21.704397775907328	27.252004563760085	29.455431298118317	21.58816636221427
100-101	21.889155121103038	26.940695712030394	29.11614939374122	22.053999773125348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51725.0
1	28043.0
2	3144.0
3	1577.5
4	1084.5
5	906.5
6	821.5
7	763.5
8	745.0
9	750.0
10	779.5
11	807.0
12	931.5
13	1420.5
14	1994.0
15	5041.0
16	116612.0
17	121186.0
18	13856.0
19	10148.5
20	9795.5
21	11090.5
22	13722.5
23	17315.5
24	22422.0
25	29232.0
26	38692.5
27	52285.5
28	69885.0
29	92590.0
30	120652.5
31	151480.5
32	183877.5
33	215796.0
34	245342.0
35	269919.0
36	286836.0
37	296639.0
38	299801.5
39	298930.5
40	297433.5
41	299424.5
42	305272.5
43	310091.5
44	312350.5
45	311113.0
46	303619.0
47	291116.5
48	277165.0
49	258747.0
50	235411.0
51	210268.0
52	182533.0
53	157101.5
54	137781.5
55	121415.0
56	106534.5
57	92756.0
58	81355.5
59	72057.5
60	61674.5
61	51971.0
62	44890.0
63	38680.5
64	32712.5
65	26922.0
66	21657.0
67	17184.5
68	13216.5
69	9955.0
70	7186.5
71	4973.0
72	3440.0
73	2263.5
74	1403.0
75	829.5
76	465.5
77	291.0
78	182.5
79	72.5
80	19.5
81	10.0
82	5.5
83	2.5
84	3.0
85	4.0
86	3.0
87	2.0
88	1.5
89	1.5
90	2.0
91	1.0
92	1.0
93	1.5
94	4.5
95	4.5
96	1.0
97	1.5
98	3.5
99	8.5
100	15.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0016481246208830522
76-77	0.0023176752481167922
78-79	0.0059808897375013895
80-81	0.005948699803499767
82-83	0.0013648532016687777
84-85	4.2490712882141196E-4
86-87	0.0014034811224707244
88-89	0.001216779505261316
90-91	7.725584160389308E-5
92-93	2.575194720129769E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7766403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.63987932840332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03588316199982	32.49404758151053
2	9.731887632557912	8.104692533145842
3	3.719408778655356	4.6462719814863895
4	2.016131616841911	3.358059089419034
5	1.2883447466172275	2.682325989126185
6	0.9267069241727393	2.3152778697209198
7	0.6889992630373488	2.008289231916381
8	0.5312085909023884	1.7695569298709295
9	0.4120546359466042	1.5442114785773182
>10	2.392311127617293	17.71322554637915
>50	0.18785286653700395	5.411591762445005
>100	0.06323740111980798	4.111961850920531
>500	0.0028311295335165902	0.8408964251905346
>1k	0.0022710404558186578	1.8341759143327327
>5k	4.3554200270573556E-4	1.3180536353742465
>10k+	4.3554200270573556E-4	9.847362180584188
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	436503	5.620401104604023	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57423	0.7393770320700587	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	38743	0.4988538452099382	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	34338	0.4421351814990801	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	28654	0.36894814755299205	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC	28078	0.3615315867590183	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT	25569	0.32922576899499034	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	22871	0.29448639222043976	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	18405	0.236982294119942	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	16836	0.21677989154052396	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	14462	0.1862123302125836	No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13401	0.17255092222229518	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG	13153	0.16935768076933427	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	11730	0.15103517033561098	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC	9759	0.1256566263687321	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT	9263	0.11927014346281026	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8429	0.10853158147986913	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTACC	8315	0.10706372048939515	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	8305	0.10693496075338868	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTATT	7945	0.10229961025715509	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTACT	7813	0.10059998174186945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0021374116177077084	0.0	0.0	1.2875973600648847E-5	0.0
2	0.002188915512110304	0.0	0.0	1.2875973600648847E-5	0.0
3	0.0022146674593116014	0.0	0.0	1.2875973600648847E-5	0.0
4	0.002253295380113548	0.0	0.0	1.2875973600648847E-5	0.0
5	0.002253295380113548	0.0	0.0	1.2875973600648847E-5	0.0
6	0.002330551221717441	0.0	0.0	1.2875973600648847E-5	0.0
7	0.0024078070633213344	0.0	0.0	1.2875973600648847E-5	0.0
8	0.002510814852126525	0.0	0.0	1.2875973600648847E-5	0.0
9	0.002703954456136258	0.0	0.0	1.2875973600648847E-5	0.0
10-11	0.002890656073345666	0.0	0.0	1.2875973600648847E-5	0.0
12-13	0.0031996794397612383	0.0	0.0	1.2875973600648847E-5	0.0
14-15	0.003399257030571295	0.0	0.0	1.2875973600648847E-5	0.0
16-17	0.0036310245553829745	0.0	0.0	1.2875973600648847E-5	0.0
18-19	0.0039915518162011426	0.0	0.0	1.2875973600648847E-5	0.0
20-21	0.004435772905423527	0.0	0.0	1.2875973600648847E-5	0.0
22-23	0.004822052113442993	0.0	0.0	1.2875973600648847E-5	0.0
24-25	0.0051954553478618095	0.0	0.0	2.5751947201297693E-5	0.0
26-27	0.0055624205954803016	0.0	0.0	2.5751947201297693E-5	0.0
28-29	0.006013079671503011	0.0	0.0	2.5751947201297693E-5	0.0
30-31	0.006367168945520854	0.0	0.0	3.218993400162212E-5	0.0
32-33	0.0068371419819445375	0.0	0.0	3.862792080194654E-5	0.0
34-35	0.007242735150364976	0.0	0.0	3.862792080194654E-5	0.0
36-37	0.007635452345184765	0.0	0.0	3.862792080194654E-5	0.0
38-39	0.007950913698400662	0.0	0.0	3.862792080194654E-5	0.0
40-41	0.008156929276011044	0.0	0.0	3.862792080194654E-5	0.0
42-43	0.00853033251042986	1.2875973600648847E-5	0.0	3.862792080194654E-5	0.0
44-45	0.008774976008842188	1.2875973600648847E-5	0.0	3.862792080194654E-5	0.0
46-47	0.00905180944125614	1.2875973600648847E-5	0.0	3.862792080194654E-5	0.0
48-49	0.009341518847270738	1.2875973600648847E-5	0.0	4.5065907602270964E-5	0.0
50-51	0.009631228253285336	1.2875973600648847E-5	0.0	6.437986800324423E-5	0.0
52-53	0.011002519441754439	1.2875973600648847E-5	0.0	6.437986800324423E-5	0.0
54-55	0.01272146191744106	1.2875973600648847E-5	0.0	6.437986800324423E-5	0.0
56-57	0.014697923865140659	1.2875973600648847E-5	0.0	7.081785480356865E-5	0.0
58-59	0.016564940037234743	1.2875973600648847E-5	0.0	7.725584160389308E-5	0.0
60-61	0.019037126968559318	1.2875973600648847E-5	0.0	7.725584160389308E-5	0.0
62-63	0.022140236606315693	1.2875973600648847E-5	0.0	7.725584160389308E-5	0.0
64-65	0.02618329231691943	1.2875973600648847E-5	0.0	7.725584160389308E-5	0.0
66-67	0.030760700931950095	2.5751947201297693E-5	0.0	7.725584160389308E-5	0.0
68-69	0.0362458656858265	2.5751947201297693E-5	0.0	7.725584160389308E-5	0.0
70-71	0.04261947261814768	2.5751947201297693E-5	0.0	7.725584160389308E-5	0.0
72-73	0.04993946361011655	2.5751947201297693E-5	0.0	7.725584160389308E-5	0.0
74-75	0.05939686621979313	2.5751947201297693E-5	0.0	1.0300778880519077E-4	0.0
76-77	0.07124919991919039	2.5751947201297693E-5	0.0	1.0300778880519077E-4	0.0
78-79	0.08536770497230184	2.5751947201297693E-5	0.0	1.0300778880519077E-4	0.0
80-81	0.10196483494353821	2.5751947201297693E-5	0.0	1.1588376240583962E-4	0.0
82-83	0.12173589240733451	2.5751947201297693E-5	0.0	1.1588376240583962E-4	0.0
84-85	0.1447130672976924	2.5751947201297693E-5	0.0	1.2232174920616404E-4	0.0
86-87	0.17114100311302416	3.862792080194654E-5	0.0	1.2875973600648847E-4	0.0
88-89	0.20191458001857487	3.862792080194654E-5	0.0	1.2875973600648847E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	257700	0.0	94.57852	1
TCGACGG	260830	0.0	93.439926	2
CGACGGC	263090	0.0	92.60476	3
ATATCTC	109615	0.0	92.338135	1
GACGGCG	264520	0.0	92.11491	4
ACGGCGC	264800	0.0	92.001366	5
CGGCGCG	266270	0.0	91.46313	6
TATCTCG	111275	0.0	90.71732	2
ATCTCGA	114610	0.0	88.01955	3
TCTCGAG	114055	0.0	86.82783	4
CTCGAGG	115940	0.0	85.50627	5
TCGAGGG	117970	0.0	84.115425	6
AGGGCGC	120195	0.0	83.06811	9
CGAGGGC	120210	0.0	82.571724	7
GAGGGCG	121335	0.0	82.14671	8
CTCGACG	2415	0.0	73.56013	5
TCTCGAC	2785	0.0	63.787327	4
GGCGCGC	386745	0.0	63.379192	7
CGCGCCG	386685	0.0	63.34603	9
GCGCGCC	386860	0.0	63.330883	8
>>END_MODULE