FastQCFastQC Report
Thu 26 May 2016
SRR1051297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7766403
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2697913.473821793692653No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT167600.21580131754687465No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT139380.17946532004584362No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128750.1657781601083539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG56850.088.871881
TCGACGG62600.080.884932
CGACGGC86700.058.4013443
GACGGCG88050.057.559874
TATCTCG77700.056.9132542
ACGGCGC92050.055.213435
ATATCTC81800.054.4368321
CGGCGCG120900.042.1165966
ATCTCGA127500.041.6413041
TCTCGAG137950.038.426182
CTCGAGG161750.032.889593
TCGAGGG169350.031.7221164
AGGGCGC181200.029.5951467
GGGCGCG183850.029.1685668
CGAGGGC187050.028.8473155
GAGGGCG207700.025.9563796
CGCGCCG299700.022.6797189
GCGCGCC308750.022.0610898
GGCGCGC308800.021.9959897
GGATCCA137050.020.82956514-15
Produced by FastQC (version 0.11.5)