FastQCFastQC Report
Thu 26 May 2016
SRR1051296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5060738
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2998745.925499403446691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541621.0702391627466192No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT206380.4078061342041418No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA175520.34682688572299136No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA151170.29871137371663975No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC131280.2594088055931763No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT122710.2424745165626041No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC112330.22196367407283285No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC92770.1833131847568477No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92290.18236470649142478No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG83170.16434361944838877No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT77350.1528433204801355No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA76720.1515984427567679No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT76140.15045236485271515No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG61340.12120761833550758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1352450.094.415871
TCGACGG1369900.093.2131962
CGACGGC1388550.091.916753
ATATCTC634200.091.725911
GACGGCG1397550.091.314624
ACGGCGC1397250.091.286645
CGGCGCG1406200.090.6752246
TATCTCG644350.090.1262052
ATCTCGA666050.087.147093
TCTCGAG666200.085.722874
CTCGAGG681800.083.900825
TCGAGGG696050.082.265036
AGGGCGC716150.080.526539
CGAGGGC718450.079.832387
GAGGGCG728900.079.026728
CTCGACG13850.065.8475955
TCTCGAC14600.061.814344
GGCGCGC2124200.060.656717
CGCGCCG2125150.060.6318329
GCGCGCC2127250.060.576448
Produced by FastQC (version 0.11.5)