##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051296_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5060738
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.943687066985092	31.0	31.0	34.0	30.0	34.0
2	32.267038918039226	33.0	31.0	34.0	31.0	34.0
3	32.31392397709583	34.0	31.0	34.0	31.0	34.0
4	36.02399808091231	37.0	35.0	37.0	35.0	37.0
5	35.8826564425979	37.0	35.0	37.0	35.0	37.0
6	35.94836642402748	37.0	36.0	37.0	35.0	37.0
7	35.88210869639962	37.0	35.0	37.0	35.0	37.0
8	35.97039167014771	37.0	36.0	37.0	35.0	37.0
9	37.57545263161223	39.0	38.0	39.0	35.0	39.0
10-11	37.58966221922573	39.0	38.0	39.0	35.0	39.0
12-13	37.608691854824336	39.0	38.0	39.0	35.0	39.0
14-15	38.99738328678545	41.0	39.0	41.0	35.5	41.0
16-17	38.82760083608359	40.5	38.5	41.0	35.0	41.0
18-19	38.72891196896579	40.5	38.5	41.0	34.5	41.0
20-21	38.85981007117934	40.5	39.0	41.0	35.0	41.0
22-23	38.81901977537663	40.5	38.5	41.0	34.5	41.0
24-25	38.808359373672374	40.5	38.5	41.0	34.5	41.0
26-27	38.900102415892704	41.0	39.0	41.0	35.0	41.0
28-29	38.51197404410187	40.0	38.5	41.0	34.5	41.0
30-31	37.359834771134175	38.0	36.0	40.5	34.0	41.0
32-33	36.75710331180946	36.0	35.0	40.0	34.0	41.0
34-35	36.4631019230792	35.0	35.0	40.0	33.0	41.0
36-37	36.34724767415345	35.0	35.0	40.0	33.0	41.0
38-39	35.80878322489724	35.0	35.0	40.0	31.5	41.0
40-41	33.33422141197588	35.0	31.5	40.0	18.0	41.0
42-43	33.41662717967222	35.0	31.0	40.0	18.0	41.0
44-45	33.461148650651346	35.0	31.0	40.0	20.0	41.0
46-47	33.735470004572456	35.0	32.0	40.0	23.0	41.0
48-49	33.276193412897484	35.0	31.0	40.0	19.0	41.0
50-51	33.37003199928548	35.0	31.0	40.0	20.0	41.0
52-53	33.277834280296666	35.0	31.0	40.0	20.0	41.0
54-55	32.86949759106281	35.0	31.0	40.0	18.0	41.0
56-57	32.50238967122977	35.0	31.0	40.0	18.0	41.0
58-59	32.1342948400016	35.0	31.0	39.5	18.0	41.0
60-61	31.810934590962823	35.0	30.5	39.0	16.0	41.0
62-63	31.437093364643655	35.0	30.0	39.0	11.5	41.0
64-65	31.03976495127786	35.0	29.5	38.5	8.5	41.0
66-67	30.620351320301506	35.0	29.0	37.5	7.0	40.0
68-69	30.256992458412192	35.0	29.0	37.0	7.0	40.0
70-71	29.86602299901714	34.5	29.0	36.5	2.0	39.5
72-73	29.496486382025708	34.0	29.0	36.0	2.0	39.0
74-75	29.156156473621042	34.0	28.5	35.5	2.0	39.0
76-77	28.83954474624057	34.0	27.5	35.0	2.0	37.5
78-79	28.60231679648304	34.0	27.0	35.0	2.0	37.0
80-81	28.377161295447422	34.0	27.0	35.0	2.0	36.5
82-83	28.206416238105984	34.0	27.0	35.0	2.0	36.0
84-85	28.01242951126891	34.0	27.0	35.0	2.0	36.0
86-87	27.799920979904513	34.0	27.0	35.0	2.0	35.5
88-89	27.578752347977705	34.0	27.0	35.0	2.0	35.0
90-91	27.345358424008513	34.0	26.5	35.0	2.0	35.0
92-93	27.043494446857355	34.0	25.0	35.0	2.0	35.0
94-95	26.7371076115776	34.0	25.0	35.0	2.0	35.0
96-97	26.386248013629633	34.0	25.0	35.0	2.0	35.0
98-99	25.95990219213087	33.5	23.5	35.0	2.0	35.0
100-101	24.26458532332636	31.5	10.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20699394464514143
1101	2	0.20714457340767467
1101	3	0.24694123979493554
1101	4	0.1803763959573388
1101	5	0.14544802902511123
1101	6	0.1942528341246046
1101	7	0.2389450953956569
1101	8	0.15630324505863769
1101	9	0.2668993481588018
1101	10-11	0.20084097562349257
1101	12-13	0.2072919545463705
1101	14-15	0.28346280283452785
1101	16-17	0.3310916659895753
1101	18-19	0.3275974092527463
1101	20-21	0.24594029800275052
1101	22-23	0.27698315768550685
1101	24-25	0.29575158484376374
1101	26-27	0.3000190177017217
1101	28-29	0.2500814806685483
1101	30-31	0.2786576189125256
1101	32-33	0.2227422107990762
1101	34-35	0.2509209973133153
1101	36-37	0.21918246744377257
1101	38-39	0.18527316322072807
1101	40-41	-0.2287902383298004
1101	42-43	-0.0824391992656075
1101	44-45	-0.10804175676136651
1101	46-47	-0.02905477709149551
1101	48-49	0.13235636935935702
1101	50-51	0.05429944256868424
1101	52-53	-0.13609821030982516
1101	54-55	-0.283845481242075
1101	56-57	-0.2263366828293485
1101	58-59	-0.18252560558769915
1101	60-61	-0.22254108379266313
1101	62-63	-0.35207042561982504
1101	64-65	-0.34609820999379437
1101	66-67	-0.31194852128028216
1101	68-69	-0.3200555385339534
1101	70-71	-0.3819598083284639
1101	72-73	-0.42996157978858207
1101	74-75	-0.5446973072045438
1101	76-77	-0.472377990501343
1101	78-79	-0.45925144977667287
1101	80-81	-0.42444064835421
1101	82-83	-0.472968098853368
1101	84-85	-0.48767525890842833
1101	86-87	-0.6431175745919653
1101	88-89	-0.5658075922315682
1101	90-91	-0.4989617881662127
1101	92-93	-0.5172336063680305
1101	94-95	-0.4915178233228197
1101	96-97	-0.5714795160511628
1101	98-99	-0.6504278053267107
1101	100-101	-0.5691988744218932
1102	1	0.10978155320072602
1102	2	0.18253123402572413
1102	3	0.14079587371453073
1102	4	0.09728059100124398
1102	5	0.13612626466259314
1102	6	0.17340115250750188
1102	7	0.13674360642013994
1102	8	0.10208502446991474
1102	9	0.19370155440404346
1102	10-11	0.1500614995455365
1102	12-13	0.13732605005459675
1102	14-15	0.22843927935608832
1102	16-17	0.24626333461847594
1102	18-19	0.2721028170780073
1102	20-21	0.22188447812654033
1102	22-23	0.2551132071966009
1102	24-25	0.20046985463078926
1102	26-27	0.19933407514280788
1102	28-29	0.2468871839078446
1102	30-31	0.18832277162409383
1102	32-33	0.2305374402214042
1102	34-35	0.25381738429066303
1102	36-37	0.2547971777253082
1102	38-39	0.14806171347262875
1102	40-41	-0.17352716487781805
1102	42-43	-0.193189201204504
1102	44-45	-0.19933500610362387
1102	46-47	-0.06496261883785337
1102	48-49	0.02236603736803744
1102	50-51	0.0624646333046428
1102	52-53	0.04292017088921085
1102	54-55	-0.07836921024333776
1102	56-57	-0.215879222416703
1102	58-59	-0.20012133175667657
1102	60-61	-0.30401465879056033
1102	62-63	-0.3498610965262543
1102	64-65	-0.41639569303655577
1102	66-67	-0.33778297834549065
1102	68-69	-0.3519103369747363
1102	70-71	-0.3065615713148233
1102	72-73	-0.4752887738647331
1102	74-75	-0.42811737988423815
1102	76-77	-0.36404715016382383
1102	78-79	-0.4608507713730603
1102	80-81	-0.3728419479392322
1102	82-83	-0.4365118715409615
1102	84-85	-0.48469071195385993
1102	86-87	-0.5646281146621561
1102	88-89	-0.5397323722350258
1102	90-91	-0.44189795942379817
1102	92-93	-0.5293487941322432
1102	94-95	-0.48347554564245243
1102	96-97	-0.4515460820717365
1102	98-99	-0.4365885855276268
1102	100-101	-0.2817498962965601
1103	1	0.10249000724236623
1103	2	0.11299852488807716
1103	3	0.13085476130567741
1103	4	0.11175191570944065
1103	5	0.1720822369414421
1103	6	0.20520851052465616
1103	7	0.2267104291774089
1103	8	0.13332332010612902
1103	9	0.20761376517999253
1103	10-11	0.1740146684738093
1103	12-13	0.19598855059994946
1103	14-15	0.23960977548077977
1103	16-17	0.28828769386751674
1103	18-19	0.3054308982973666
1103	20-21	0.28282381176388327
1103	22-23	0.2734851852511042
1103	24-25	0.2575491623854944
1103	26-27	0.21429893183096027
1103	28-29	0.1433333923956539
1103	30-31	0.17297381589163763
1103	32-33	0.16708748494774284
1103	34-35	0.2012980786034504
1103	36-37	0.21907429926673672
1103	38-39	0.1870143085588154
1103	40-41	-0.1532807379131853
1103	42-43	-0.18443373268397067
1103	44-45	-0.21953769324075267
1103	46-47	-0.0537680018934239
1103	48-49	0.037867276616829315
1103	50-51	0.01779996132972883
1103	52-53	-0.03860719943290292
1103	54-55	-0.07006656287222057
1103	56-57	-0.12529692971055795
1103	58-59	-0.08895555975566793
1103	60-61	-0.15599235759210472
1103	62-63	-0.1451398628963858
1103	64-65	-0.28814254336603184
1103	66-67	-0.29644878577742517
1103	68-69	-0.27966503601887993
1103	70-71	-0.41314499892221335
1103	72-73	-0.4632333937964752
1103	74-75	-0.42902766293983774
1103	76-77	-0.37016086093240474
1103	78-79	-0.4238980367862766
1103	80-81	-0.37933722895650845
1103	82-83	-0.3562046312100655
1103	84-85	-0.3974743295821739
1103	86-87	-0.40422330172494725
1103	88-89	-0.4939608102924531
1103	90-91	-0.492628044949889
1103	92-93	-0.45502963066894964
1103	94-95	-0.4590051963268529
1103	96-97	-0.4366413884881233
1103	98-99	-0.42353160787189736
1103	100-101	-0.3527206008940489
1104	1	0.06758495266868891
1104	2	0.12166517530707921
1104	3	0.09887500589206866
1104	4	0.14179869789698074
1104	5	0.12491719541019819
1104	6	0.14463019347221717
1104	7	0.16505557529927728
1104	8	0.11505607413646857
1104	9	0.16149141733380645
1104	10-11	0.18541433251647277
1104	12-13	0.15180986620265458
1104	14-15	0.20336033768679584
1104	16-17	0.1627256355546649
1104	18-19	0.27765797635482414
1104	20-21	0.25342649292345953
1104	22-23	0.28892244859837035
1104	24-25	0.2774328937496122
1104	26-27	0.2411972218384264
1104	28-29	0.21178412616220754
1104	30-31	0.24452884081333082
1104	32-33	0.2442700351807119
1104	34-35	0.2263239733141731
1104	36-37	0.22505766683093498
1104	38-39	0.21845923895605068
1104	40-41	-0.05999164068496299
1104	42-43	-0.004123507587394215
1104	44-45	-0.09309929514202508
1104	46-47	0.03176220915631944
1104	48-49	0.071861908241722
1104	50-51	0.1365656412463636
1104	52-53	0.019717308466049133
1104	54-55	-0.024293945942595485
1104	56-57	-0.03822894210900074
1104	58-59	-0.08078968811662435
1104	60-61	-0.00746462652635671
1104	62-63	-0.013084260444170326
1104	64-65	-0.01937127831571317
1104	66-67	-0.1494007643364199
1104	68-69	-0.14163576844131143
1104	70-71	-0.18762628309307416
1104	72-73	-0.16745627183340162
1104	74-75	-0.15909865045055227
1104	76-77	-0.18069687867485484
1104	78-79	-0.1340896871868189
1104	80-81	-0.1558690326463541
1104	82-83	-0.20822458481560346
1104	84-85	-0.1678248765744783
1104	86-87	-0.08742364425519611
1104	88-89	-0.08520716103072346
1104	90-91	-0.05522503241186172
1104	92-93	0.008820577707311372
1104	94-95	0.016760040841003132
1104	96-97	0.05423980528665595
1104	98-99	-0.00928700597284049
1104	100-101	0.11370961613778263
1105	1	-0.010894430924839327
1105	2	0.08639076073270502
1105	3	0.03141160380381791
1105	4	0.06293568568013086
1105	5	0.03451806629176701
1105	6	0.12721982620714556
1105	7	0.11728856143456312
1105	8	0.09951271388919736
1105	9	0.13736985064456775
1105	10-11	0.09952161705873408
1105	12-13	0.10555010862947967
1105	14-15	0.18578318474915534
1105	16-17	0.15469280891221615
1105	18-19	0.24623631800898238
1105	20-21	0.19513874815343968
1105	22-23	0.19486629727809657
1105	24-25	0.1459852502103658
1105	26-27	0.15818337524729742
1105	28-29	0.1657174055640951
1105	30-31	0.18816308695360107
1105	32-33	0.15297746276019808
1105	34-35	0.14088608864269503
1105	36-37	0.13299312631359328
1105	38-39	0.148545259799441
1105	40-41	-0.11461632185750403
1105	42-43	-0.13452447933150324
1105	44-45	-0.13307161404256362
1105	46-47	-0.14265040176802302
1105	48-49	-0.05032589400786236
1105	50-51	0.020980293299373898
1105	52-53	-0.0054923567313096555
1105	54-55	-0.08100344802227966
1105	56-57	-0.09618908500814882
1105	58-59	-0.01140353994362897
1105	60-61	-0.07703111755143155
1105	62-63	-0.14864756880448127
1105	64-65	-0.2621647204095119
1105	66-67	-0.10448521729379934
1105	68-69	-0.2410502061686195
1105	70-71	-0.2797201315714233
1105	72-73	-0.3146642983741188
1105	74-75	-0.3116110942871515
1105	76-77	-0.29521016658072696
1105	78-79	-0.3658663566402005
1105	80-81	-0.3958204655790958
1105	82-83	-0.35502496099317327
1105	84-85	-0.2772129752552104
1105	86-87	-0.322959176579829
1105	88-89	-0.3307437923139922
1105	90-91	-0.3590282276197101
1105	92-93	-0.2610334621739554
1105	94-95	-0.1835262853517854
1105	96-97	-0.23474269566701977
1105	98-99	-0.30515889156146514
1105	100-101	-0.2931671247191403
1106	1	-0.0013066424095562468
1106	2	0.029856540335529758
1106	3	0.00818221193876667
1106	4	0.029272003305855776
1106	5	0.03664916607529989
1106	6	0.08823536376308994
1106	7	0.06353366369641122
1106	8	0.05127199676694261
1106	9	0.1270183564772438
1106	10-11	0.09390836657749446
1106	12-13	0.05170955709990466
1106	14-15	0.10717293668071903
1106	16-17	0.11267352600660274
1106	18-19	0.160945527220278
1106	20-21	0.08860640949131238
1106	22-23	0.14821547203274577
1106	24-25	0.14241744572124304
1106	26-27	0.13508295192659858
1106	28-29	0.12471418988798888
1106	30-31	0.08275944084802234
1106	32-33	0.05796151679805206
1106	34-35	0.12701181223950897
1106	36-37	0.06754941319037755
1106	38-39	0.04596355416691722
1106	40-41	-0.37571148468334314
1106	42-43	-0.29545722054554346
1106	44-45	-0.22666040336211069
1106	46-47	-0.19556939098358583
1106	48-49	-0.15791674347881468
1106	50-51	0.0041483127244532625
1106	52-53	0.08970960222455915
1106	54-55	-0.028562663436332514
1106	56-57	-0.03605043598239632
1106	58-59	-0.0635134179375072
1106	60-61	-0.12397350718767086
1106	62-63	-0.14613458134799018
1106	64-65	-0.17711757946198148
1106	66-67	-0.18799965020157927
1106	68-69	-0.13811403740904282
1106	70-71	-0.10755925158848356
1106	72-73	-0.1215581392462397
1106	74-75	-0.1572283939834236
1106	76-77	-0.19885794480919472
1106	78-79	-0.16315888838228432
1106	80-81	-0.2181678812599408
1106	82-83	-0.168568365651236
1106	84-85	-0.22399022521792133
1106	86-87	-0.23208266439663916
1106	88-89	-0.22423685707494911
1106	90-91	-0.20070454689021133
1106	92-93	-0.1933071695962134
1106	94-95	-0.20290846992072176
1106	96-97	-0.2459990953085125
1106	98-99	-0.2925473290662559
1106	100-101	-0.23322462914818587
1107	1	0.06893998877271912
1107	2	-0.017624787393138774
1107	3	0.0274958007283459
1107	4	0.04981760565902249
1107	5	0.0863172202682776
1107	6	0.04535474797284422
1107	7	0.03452604338416876
1107	8	0.046993648554106926
1107	9	0.08986472229383935
1107	10-11	0.06882630508644638
1107	12-13	0.06982230819050983
1107	14-15	0.05602237933435106
1107	16-17	0.08078732567017255
1107	18-19	0.05507032172555171
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2216	10-11	-0.3573055778195027
2216	12-13	-0.3244982117567474
2216	14-15	-0.4981674795533877
2216	16-17	-0.48511856359712624
2216	18-19	-0.4612426241377676
2216	20-21	-0.4276421233133192
2216	22-23	-0.4001436505723177
2216	24-25	-0.40010747305493055
2216	26-27	-0.2910950693925969
2216	28-29	-0.35896208102206373
2216	30-31	-0.433703688619822
2216	32-33	-0.659603845712887
2216	34-35	-0.6922444374342192
2216	36-37	-0.750446757167353
2216	38-39	-0.6554949362457876
2216	40-41	0.22066768434456918
2216	42-43	0.17716671676370765
2216	44-45	0.07182371002673449
2216	46-47	-0.1266700983049489
2216	48-49	-0.6655624525949904
2216	50-51	-0.7844899981213018
2216	52-53	-0.7172303193205138
2216	54-55	-0.6392621974319397
2216	56-57	-0.7907270421745132
2216	58-59	-0.6135745544414313
2216	60-61	-0.6061166699361635
2216	62-63	-0.5811484554595303
2216	64-65	-0.4796529145852837
2216	66-67	-0.36901458612934235
2216	68-69	-0.34882050038782353
2216	70-71	-0.32616373285116396
2216	72-73	-0.254038857836953
2216	74-75	-0.2318998525464906
2216	76-77	-0.1229834667701688
2216	78-79	-0.13104675133318366
2216	80-81	-0.1272131777047143
2216	82-83	-0.19143246286258275
2216	84-85	-0.1778487943115863
2216	86-87	-0.19802629503313085
2216	88-89	-0.1355067742016196
2216	90-91	-0.07962820806266535
2216	92-93	-0.006381286305582989
2216	94-95	-0.12158802637969401
2216	96-97	-0.16990135601372458
2216	98-99	-0.20146782308795608
2216	100-101	-0.2825190972094731
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	21.0
11	363.0
12	1408.0
13	3018.0
14	4709.0
15	6911.0
16	10460.0
17	18498.0
18	45255.0
19	140439.0
20	150100.0
21	99417.0
22	84692.0
23	77657.0
24	76524.0
25	84089.0
26	99584.0
27	121557.0
28	144137.0
29	162410.0
30	176656.0
31	199472.0
32	232406.0
33	269471.0
34	314514.0
35	420125.0
36	549287.0
37	740863.0
38	712689.0
39	113987.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.31181578655129	32.24008039934097	16.18252120540522	12.265582608702525
2	13.923068137492988	19.311551003035525	56.08259111615737	10.682789743314117
3	13.888191801274836	30.854946452473932	16.590603979103445	38.66625776714779
4	41.27544243547087	17.447988811118062	27.985720659714058	13.290848093697004
5	15.085566571515855	43.477729927927506	15.366592777575129	26.07011072298151
6	15.200984520439508	16.76176478608456	26.76692608864557	41.270324604830364
7	41.73614599293621	17.02405854640173	15.129078011942132	26.110717448719928
8	53.650060524769316	17.052947613569405	15.170238016668716	14.126753844992567
9	15.054839827708925	28.39777518614874	15.55211907828463	40.99526590785771
10-11	40.376176360048674	16.525939497361847	15.360625268488509	27.737258874100974
12-13	33.93744153520692	16.66907079560333	15.61243636797637	33.78105130121338
14-15	33.576901234563024	16.914795826221393	15.838440954659184	33.669861984556405
16-17	33.287378244042664	30.15225249756063	15.91213573988616	20.64823351851054
18-19	20.233768276484575	17.223871300984165	29.395702761138793	33.14665766139247
20-21	19.41640725127442	36.366573412810546	17.097417412243036	27.119601923672004
22-23	13.19586194740767	29.86414234445648	36.71113778267122	20.228857925464627
24-25	13.191909954635076	17.059962400740762	50.255298733109676	19.492828911514486
26-27	13.188442080977122	16.972159001315617	56.573438498495676	13.265960419211586
28-29	13.273321005750544	16.54468182308588	57.013779413200204	13.168217757963363
30-31	13.207322726448197	16.472360355347384	57.088057117361146	13.232259800843277
32-33	13.204032692465011	16.45908165963146	57.10425040774686	13.232635240156673
34-35	13.250310527832108	16.543189945814227	56.9929721712525	13.213527355101174
36-37	13.346916991158206	16.51030936594623	56.815517815781014	13.327255827114543
38-39	14.135082669760813	17.00012923016366	54.30199113251861	14.562796967556906
40-41	18.727644070884523	20.394732942112395	41.46537520812182	19.412247778881262
42-43	18.802514573961346	21.456791479819742	42.505124746627864	17.235569199591048
44-45	19.966149205906333	23.676329420728756	39.09664954004732	17.260871833317594
46-47	19.026879083643532	25.78433224561319	37.839599678940104	17.34918899180317
48-49	18.956889291640863	27.453100713769413	35.536793250312506	18.05321674427722
50-51	19.263771410414847	27.488421649174487	34.48016870266748	18.767638237743192
52-53	19.31868434999006	26.981430376360127	34.60148500080423	19.09840027284558
54-55	19.415043813767873	26.04384973100761	35.05720904737609	19.48389740784842
56-57	19.233123706463363	25.782603248775178	35.653841396254855	19.3304316485066
58-59	19.334097121803183	25.787424679957745	35.69214608620324	19.186332112035835
60-61	19.835170285440583	25.904255466297606	35.010911452045136	19.24966279621668
62-63	20.23978518548085	26.157064443960543	34.133410976818006	19.469739393740596
64-65	20.389180392266898	26.40849022415308	33.23312726325686	19.969202120323164
66-67	20.51962579370835	26.589234613607736	32.59456427106086	20.29657532162305
68-69	20.53939563755326	26.916548535016037	32.16731433241555	20.376741495015153
70-71	20.863429404960304	27.251371242692272	31.6879376090997	20.197261743247726
72-73	20.927876527099407	27.48034970393646	31.331043021788524	20.26073074717561
74-75	21.145371837846632	27.554672533893104	31.01863227743215	20.28132335082811
76-77	21.20601433245765	27.75647765097163	30.69886909911974	20.338638917450986
78-79	21.145296361830543	27.649759841214195	30.63097556281102	20.573968234144242
80-81	21.13506781328165	27.514135486258258	30.662570813290053	20.68822588717004
82-83	21.15787040055961	27.41721945908348	30.753171022809234	20.671739117547677
84-85	21.206341703812225	27.16710184831334	30.99027174383418	20.636284704040254
86-87	21.097646390347055	27.105967219580556	31.17080167293938	20.625584717133005
88-89	21.153756283113704	27.04600906497388	30.978389793876822	20.821844858035593
90-91	21.358627431898444	26.972162626655333	30.686931190297056	20.982278751149167
92-93	21.48757611541088	27.04535466777955	30.547700966815896	20.919368249993678
94-95	21.441941866976713	27.089724858311182	30.544655739933585	20.923677534778523
96-97	21.375281628884956	27.20044981581738	30.28578045336471	21.13848810193296
98-99	21.548378912324644	27.024003218502912	30.362794912520663	21.064822956651778
100-101	21.713394370544375	26.695049220093985	30.08272706470874	21.508829344652895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46950.0
1	25727.5
2	3277.5
3	1616.0
4	1108.0
5	993.5
6	913.5
7	838.5
8	821.0
9	846.5
10	908.5
11	982.0
12	1138.5
13	1612.5
14	2098.0
15	5747.0
16	109731.0
17	111929.5
18	9896.5
19	5630.0
20	5275.0
21	6058.0
22	7620.5
23	9759.0
24	12657.5
25	16770.5
26	22346.0
27	30312.5
28	40823.5
29	54084.0
30	70195.0
31	88129.5
32	107951.5
33	127388.0
34	143186.0
35	156690.5
36	169153.5
37	179034.5
38	183548.5
39	183108.0
40	182759.0
41	185691.5
42	192450.5
43	199793.5
44	203354.5
45	202135.0
46	197088.0
47	190551.5
48	183813.0
49	173213.5
50	158769.0
51	143998.5
52	126429.5
53	108687.0
54	95542.0
55	84833.5
56	74485.5
57	64894.5
58	57705.0
59	51712.0
60	45068.5
61	39103.0
62	33797.0
63	28901.5
64	25028.5
65	21118.5
66	17093.5
67	13496.0
68	10452.5
69	7975.0
70	5757.5
71	4013.5
72	2767.0
73	1847.0
74	1207.5
75	751.0
76	429.0
77	275.5
78	189.5
79	82.0
80	28.5
81	14.0
82	6.5
83	2.0
84	2.0
85	2.0
86	1.0
87	0.5
88	0.5
89	0.5
90	0.5
91	0.5
92	2.0
93	2.0
94	1.5
95	1.0
96	0.5
97	1.0
98	3.0
99	4.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0016697169464216484
76-77	0.002499635428666728
78-79	0.006639347857960638
80-81	0.0066294678760291485
82-83	0.00134367754268251
84-85	3.161594218076494E-4
86-87	0.0012745176691620866
88-89	9.978781750803935E-4
90-91	1.0867980124637949E-4
92-93	3.9519927725956175E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5060738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.55845375353731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16667346811018	37.789948129934714
2	8.623556162807196	8.02998881594181
3	3.1492476556079945	4.398723039961818
4	1.690215962658238	3.147753669236565
5	1.1192574431780373	2.6055447953253537
6	0.7721611294059665	2.15703769402361
7	0.5456578579803549	1.7783490302222809
8	0.43399453162766294	1.6164891464059707
9	0.33558451673297846	1.4061866582443991
>10	1.9661346823063224	16.941014125516336
>50	0.1579643822873351	5.016759337822325
>100	0.034907533206874626	2.6343127180981267
>500	0.0019175266551558602	0.5784955516273105
>1k	0.002087972255381654	1.9806603803539329
>5k	2.982817507686647E-4	1.1105665024432883
>10k+	3.408934294499025E-4	8.80817040484224
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	299874	5.925499403446691	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54162	1.0702391627466192	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	20638	0.4078061342041418	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	17552	0.34682688572299136	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	15117	0.29871137371663975	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC	13128	0.2594088055931763	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT	12271	0.2424745165626041	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	11233	0.22196367407283285	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	9277	0.1833131847568477	No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9229	0.18236470649142478	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	8317	0.16434361944838877	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	7735	0.1528433204801355	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	7672	0.1515984427567679	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7614	0.15045236485271515	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG	6134	0.12120761833550758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0023514356996943925	0.0	0.0	0.0	0.0
2	0.002430475555146305	0.0	0.0	0.0	0.0
3	0.002450235519009283	0.0	0.0	0.0	0.0
4	0.0025292753744611952	0.0	0.0	0.0	0.0
5	0.0027663949408169323	0.0	0.0	0.0	0.0
6	0.0028651947601318227	0.0	0.0	0.0	0.0
7	0.002983754543309691	0.0	0.0	0.0	0.0
8	0.0031220742903505377	0.0	0.0	0.0	0.0
9	0.0034777536398841436	0.0	0.0	0.0	0.0
10-11	0.0036951132423769023	0.0	0.0	0.0	0.0
12-13	0.00393223280873264	0.0	0.0	0.0	0.0
14-15	0.004218752284745822	0.0	0.0	0.0	0.0
16-17	0.00454479168848496	0.0	0.0	0.0	0.0
18-19	0.005009150839264944	0.0	0.0	0.0	0.0
20-21	0.005424110080387484	0.0	0.0	0.0	0.0
22-23	0.005858829285373002	0.0	0.0	0.0	0.0
24-25	0.006501028110919791	0.0	0.0	0.0	0.0
26-27	0.007014787171357222	0.0	0.0	0.0	0.0
28-29	0.00763722603304103	0.0	0.0	0.0	0.0
30-31	0.008160865075409949	0.0	0.0	0.0	0.0
32-33	0.008911743702203118	0.0	0.0	0.0	0.0
34-35	0.009642862365133308	0.0	0.0	0.0	0.0
36-37	0.010186261371365204	0.0	0.0	0.0	0.0
38-39	0.010591340630556254	0.0	0.0	0.0	0.0
40-41	0.011124859654856663	0.0	0.0	0.0	0.0
42-43	0.011648498697225583	0.0	0.0	0.0	0.0
44-45	0.012132617811868546	0.0	0.0	0.0	0.0
46-47	0.012458657215607684	0.0	0.0	0.0	0.0
48-49	0.012952656312182136	0.0	0.0	9.879981931489044E-6	0.0
50-51	0.013417015462962121	0.0	0.0	1.383197470408466E-4	0.0
52-53	0.014819972897233566	0.0	0.0	2.766394940816932E-4	0.0
54-55	0.01671692942807946	1.9759963862978088E-5	0.0	2.766394940816932E-4	0.0
56-57	0.01838664637450111	1.9759963862978088E-5	0.0	2.766394940816932E-4	0.0
58-59	0.02014528315830616	1.9759963862978088E-5	0.0	2.766394940816932E-4	0.0
60-61	0.022141039508466947	1.9759963862978088E-5	0.0	2.766394940816932E-4	0.0
62-63	0.02439367538884645	1.9759963862978088E-5	0.0	2.766394940816932E-4	0.0
64-65	0.027446589805676564	1.9759963862978088E-5	0.0	2.766394940816932E-4	0.0
66-67	0.030657583933410504	1.9759963862978088E-5	0.0	2.8651947601318227E-4	0.0
68-69	0.03438233712158187	1.9759963862978088E-5	0.0	2.963994579446713E-4	0.0
70-71	0.03853192953280727	1.9759963862978088E-5	0.0	2.963994579446713E-4	0.0
72-73	0.043896759721605824	1.9759963862978088E-5	0.0	2.963994579446713E-4	0.0
74-75	0.0502298281396903	1.9759963862978088E-5	0.0	2.963994579446713E-4	0.0
76-77	0.05839069321510025	2.963994579446713E-5	0.0	2.963994579446713E-4	0.0
78-79	0.0688041941708897	3.9519927725956175E-5	0.0	2.963994579446713E-4	0.0
80-81	0.08180625039272929	3.9519927725956175E-5	0.0	2.963994579446713E-4	0.0
82-83	0.0980291807242343	3.9519927725956175E-5	0.0	2.963994579446713E-4	9.879981931489044E-6
84-85	0.11728526550870644	3.9519927725956175E-5	0.0	2.963994579446713E-4	1.9759963862978088E-5
86-87	0.14095770221655418	3.9519927725956175E-5	0.0	2.963994579446713E-4	1.9759963862978088E-5
88-89	0.17087428750510303	3.9519927725956175E-5	0.0	2.963994579446713E-4	1.9759963862978088E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	135245	0.0	94.41587	1
TCGACGG	136990	0.0	93.213196	2
CGACGGC	138855	0.0	91.91675	3
ATATCTC	63420	0.0	91.72591	1
GACGGCG	139755	0.0	91.31462	4
ACGGCGC	139725	0.0	91.28664	5
CGGCGCG	140620	0.0	90.675224	6
TATCTCG	64435	0.0	90.126205	2
ATCTCGA	66605	0.0	87.14709	3
TCTCGAG	66620	0.0	85.72287	4
CTCGAGG	68180	0.0	83.90082	5
TCGAGGG	69605	0.0	82.26503	6
AGGGCGC	71615	0.0	80.52653	9
CGAGGGC	71845	0.0	79.83238	7
GAGGGCG	72890	0.0	79.02672	8
CTCGACG	1385	0.0	65.847595	5
TCTCGAC	1460	0.0	61.81434	4
GGCGCGC	212420	0.0	60.65671	7
CGCGCCG	212515	0.0	60.631832	9
GCGCGCC	212725	0.0	60.57644	8
>>END_MODULE