FastQCFastQC Report
Thu 26 May 2016
SRR1051296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5060738
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2539175.017390744195807No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT120950.23899676292271996No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT107960.21332856986471144No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87020.17195120553563534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG28600.088.1758961
TCGACGG32500.077.4598542
CGACGGC50550.049.7071273
ATATCTC48300.048.7638631
GACGGCG51750.048.55454
TATCTCG48850.047.8392872
ACGGCGC53100.047.5884175
ATCTCGA84700.046.795231
TCTCGAG95350.041.44642
CGGCGCG70100.036.115486
CTCGAGG113000.034.8886223
TCGAGGG116700.033.945284
AGGGCGC130100.030.558527
GGGCGCG131550.030.1494778
CGAGGGC132950.029.903455
GAGGGCG145350.027.3196666
CGCGCCG201450.020.4425939
GGCGCGC205550.020.104167
GCGCGCC205850.020.0979378
ATCCATA82650.018.21799516-17
Produced by FastQC (version 0.11.5)