##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051296_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5060738 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41325632743683 33.0 31.0 34.0 31.0 34.0 2 32.63427567283665 34.0 31.0 34.0 31.0 34.0 3 32.66609198105098 34.0 31.0 34.0 31.0 34.0 4 36.16467242524707 37.0 35.0 37.0 35.0 37.0 5 35.93431392812669 37.0 35.0 37.0 35.0 37.0 6 35.80717851823193 37.0 35.0 37.0 33.0 37.0 7 35.67805881276605 37.0 35.0 37.0 33.0 37.0 8 35.625888556175006 37.0 35.0 37.0 33.0 37.0 9 37.167487034499715 39.0 37.0 39.0 33.0 39.0 10-11 37.14469915257419 39.0 37.0 39.0 33.0 39.0 12-13 37.199072743935766 39.0 37.0 39.0 33.0 39.0 14-15 38.595572424417156 40.0 38.0 41.0 34.0 41.0 16-17 38.69190234704899 40.0 38.0 41.0 34.5 41.0 18-19 38.6895842068884 40.0 38.0 41.0 35.0 41.0 20-21 38.782848568726536 40.0 38.0 41.0 35.0 41.0 22-23 38.7842903544898 40.0 38.0 41.0 35.0 41.0 24-25 38.71770352466379 40.0 38.0 41.0 35.0 41.0 26-27 38.531207503727714 40.0 38.0 41.0 35.0 41.0 28-29 38.32565112044923 40.0 37.0 41.0 34.5 41.0 30-31 38.2222970246632 40.0 37.0 41.0 34.5 41.0 32-33 38.306003590780634 40.0 37.0 41.0 35.0 41.0 34-35 38.314136791906634 40.0 37.0 41.0 35.0 41.0 36-37 38.3097380263511 40.0 37.0 41.0 35.0 41.0 38-39 38.27479579065346 40.0 37.0 41.0 35.0 41.0 40-41 38.19758728865237 40.0 37.0 41.0 34.0 41.0 42-43 38.124781207800126 40.0 37.0 41.0 34.0 41.0 44-45 38.00152942120299 40.0 37.0 41.0 34.0 41.0 46-47 37.79879930555583 40.0 36.5 41.0 33.0 41.0 48-49 37.658152328771024 40.0 36.0 41.0 33.0 41.0 50-51 37.58778018146761 40.0 36.0 41.0 33.0 41.0 52-53 37.416964383455536 39.5 35.0 41.0 33.0 41.0 54-55 37.24973462368532 39.0 35.0 41.0 33.0 41.0 56-57 37.13727009776044 39.0 35.0 41.0 33.0 41.0 58-59 36.88863096647169 39.0 35.0 40.0 32.5 41.0 60-61 36.59928127083441 38.0 35.0 40.0 32.0 41.0 62-63 36.28427632491545 37.5 35.0 40.0 31.5 41.0 64-65 35.96360333216222 37.0 35.0 40.0 31.0 41.0 66-67 35.578419293786794 36.5 35.0 39.5 31.0 41.0 68-69 35.168761947368154 36.0 34.0 39.0 30.5 41.0 70-71 34.85504416549523 35.5 34.0 39.0 30.0 40.0 72-73 34.502593890456296 35.0 34.0 37.5 30.0 40.0 74-75 34.18128265087029 35.0 34.0 37.0 30.0 39.0 76-77 33.907407773332665 35.0 34.0 36.5 30.0 39.0 78-79 33.636141211024956 35.0 34.0 36.0 30.0 39.0 80-81 33.40204699393646 35.0 33.0 36.0 29.5 37.0 82-83 33.177089388938924 35.0 33.0 35.5 29.0 37.0 84-85 32.95111720859685 35.0 33.0 35.0 29.0 36.5 86-87 32.75058716732619 35.0 33.0 35.0 29.0 36.0 88-89 32.55821147034287 35.0 33.0 35.0 29.0 36.0 90-91 32.37934220265898 35.0 33.0 35.0 29.0 36.0 92-93 32.198683077448386 35.0 33.0 35.0 29.0 35.5 94-95 32.03569311432443 35.0 33.0 35.0 28.5 35.0 96-97 31.901502014133115 35.0 33.0 35.0 27.5 35.0 98-99 31.720076004725 35.0 33.0 35.0 27.0 35.0 100-101 30.374040604354544 34.0 31.0 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1857643068086361 1101 2 0.1825734341469598 1101 3 0.20591372469834113 1101 4 0.17301525325478195 1101 5 0.21978029838457758 1101 6 0.22341819988342593 1101 7 0.23930645639527626 1101 8 0.28909463187599016 1101 9 0.31346682093191447 1101 10-11 0.26741929244818863 1101 12-13 0.24135495090452963 1101 14-15 0.3785013462051552 1101 16-17 0.35965758112949686 1101 18-19 0.2995932171345075 1101 20-21 0.2790714773835248 1101 22-23 0.2904461129428242 1101 24-25 0.3335603171734576 1101 26-27 0.37241004482005025 1101 28-29 0.36830214531929073 1101 30-31 0.4199276964129126 1101 32-33 0.39738239715686063 1101 34-35 0.3403276108127926 1101 36-37 0.30417982435122326 1101 38-39 0.27870387755869785 1101 40-41 0.2392921915408408 1101 42-43 0.25481878084407583 1101 44-45 0.2244272815382331 1101 46-47 0.322806157333666 1101 48-49 0.2877844831402001 1101 50-51 0.24628451909249094 1101 52-53 0.2651987740015471 1101 54-55 0.2670738962142565 1101 56-57 0.29725113901294264 1101 58-59 0.30787102730884186 1101 60-61 0.31649439725720896 1101 62-63 0.33663740298768374 1101 64-65 0.3067273356907023 1101 66-67 0.3299303161855036 1101 68-69 0.30457566670627045 1101 70-71 0.21048112983233125 1101 72-73 0.13563543746743534 1101 74-75 0.19507938546895076 1101 76-77 0.1622028566734386 1101 78-79 0.16383367167347984 1101 80-81 0.1273780184143547 1101 82-83 0.11969065477632057 1101 84-85 0.12798281017512636 1101 86-87 0.17129309685030591 1101 88-89 0.13302765687001994 1101 90-91 0.13730320058290602 1101 92-93 0.1836322634138554 1101 94-95 0.21841658148131415 1101 96-97 0.19993007751111236 1101 98-99 0.19776744251244338 1101 100-101 0.24881916754630495 1102 1 0.20239963689469676 1102 2 0.19018801556092058 1102 3 0.20494966217484745 1102 4 0.1226142475731038 1102 5 0.17247740742467244 1102 6 0.20513876065365366 1102 7 0.17620392650717065 1102 8 0.11550677295121403 1102 9 0.1436792322614835 1102 10-11 0.15925315707540477 1102 12-13 0.1438674385806067 1102 14-15 0.16813156242443483 1102 16-17 0.19748670573822835 1102 18-19 0.12529390333413204 1102 20-21 0.1804124132422089 1102 22-23 0.1945649518569894 1102 24-25 0.2227493273495469 1102 26-27 0.26796925199113986 1102 28-29 0.32578467606234085 1102 30-31 0.29291103923119977 1102 32-33 0.23465387361282097 1102 34-35 0.30200771980843655 1102 36-37 0.24435996254975123 1102 38-39 0.2808428551007154 1102 40-41 0.267504964772904 1102 42-43 0.3183634154668269 1102 44-45 0.28644163207854234 1102 46-47 0.2997519649159912 1102 48-49 0.2523803496814736 1102 50-51 0.2780921500157518 1102 52-53 0.277018183847602 1102 54-55 0.27313738948195976 1102 56-57 0.3019159633070032 1102 58-59 0.3004406500749255 1102 60-61 0.30365556071602384 1102 62-63 0.29562122628428966 1102 64-65 0.23989289307072426 1102 66-67 0.3251630704711701 1102 68-69 0.344842024149564 1102 70-71 0.3276959578516383 1102 72-73 0.2918702996164342 1102 74-75 0.24568056594151955 1102 76-77 0.21170837789188113 1102 78-79 0.2077699982999235 1102 80-81 0.17481684334790515 1102 82-83 0.15442881008733877 1102 84-85 0.18342551164824528 1102 86-87 0.19486935732571453 1102 88-89 0.15573547632735085 1102 90-91 0.1623865151613444 1102 92-93 0.21019179356252948 1102 94-95 0.2400368832041977 1102 96-97 0.26104959601244104 1102 98-99 0.24022626709827577 1102 100-101 0.29512222291713286 1103 1 0.1308368328660734 1103 2 0.17193211200418546 1103 3 0.15221199081768333 1103 4 0.11826344081783446 1103 5 0.14874313215665325 1103 6 0.17876892457240956 1103 7 0.19895230495171745 1103 8 0.1434775311814036 1103 9 0.13821881222019528 1103 10-11 0.15709820637442107 1103 12-13 0.15514119849118657 1103 14-15 0.2563898919576246 1103 16-17 0.21969258805030023 1103 18-19 0.22543623840177673 1103 20-21 0.22869698667189908 1103 22-23 0.1944337323744847 1103 24-25 0.16018403370774337 1103 26-27 0.20119684832088325 1103 28-29 0.21258775318977996 1103 30-31 0.1568398817014085 1103 32-33 0.18490159832201414 1103 34-35 0.13705068571332646 1103 36-37 0.14992859281231574 1103 38-39 0.14347826029575117 1103 40-41 0.1207741278332577 1103 42-43 0.14054620515078398 1103 44-45 0.1574118086857439 1103 46-47 0.15470919631274427 1103 48-49 0.21181291017596493 1103 50-51 0.223202501376619 1103 52-53 0.15730613863829035 1103 54-55 0.18012542438823687 1103 56-57 0.1948687379098928 1103 58-59 0.2655645568918672 1103 60-61 0.24569148017360476 1103 62-63 0.2585462758389596 1103 64-65 0.2599837574214021 1103 66-67 0.29736688250550714 1103 68-69 0.3292105380319583 1103 70-71 0.26504126711639486 1103 72-73 0.17430798078311227 1103 74-75 0.035214047612335264 1103 76-77 0.10399994191306661 1103 78-79 0.12076547898089984 1103 80-81 0.1591709347585848 1103 82-83 0.183122915020121 1103 84-85 0.08113956377644627 1103 86-87 0.1151410444575589 1103 88-89 0.15236275769699148 1103 90-91 0.20569817032476578 1103 92-93 0.19786010783680297 1103 94-95 0.17234052430863045 1103 96-97 0.15931047935132625 1103 98-99 0.1863011455701944 1103 100-101 0.22839422600948822 1104 1 0.16844575682037544 1104 2 0.1682581464447992 1104 3 0.17582571447893258 1104 4 0.10130030971618709 1104 5 0.0941959471009568 1104 6 0.1271862546237159 1104 7 0.17651155168057642 1104 8 0.11632984879955899 1104 9 0.16138251258082192 1104 10-11 0.16771550858313589 1104 12-13 0.21150621993441376 1104 14-15 0.24482965301360338 1104 16-17 0.24288326322187714 1104 18-19 0.23683518237762513 1104 20-21 0.19258014272109136 1104 22-23 0.19720050605415906 1104 24-25 0.2415676661088355 1104 26-27 0.25116991966806523 1104 28-29 0.23052795957939054 1104 30-31 0.331039924388449 1104 32-33 0.2670709436372576 1104 34-35 0.2608799216818056 1104 36-37 0.2625854750633039 1104 38-39 0.22924329621416462 1104 40-41 0.20653285855115655 1104 42-43 0.21629436381067535 1104 44-45 0.21650914214905725 1104 46-47 0.20647719115189744 1104 48-49 0.2460324703665293 1104 50-51 0.2435207192149278 1104 52-53 0.20176931405173804 1104 54-55 0.2468224354093138 1104 56-57 0.29117926321895027 1104 58-59 0.2993707894948656 1104 60-61 0.2524957516099988 1104 62-63 0.2952607567889416 1104 64-65 0.19160062685150336 1104 66-67 0.15600862599777088 1104 68-69 0.22195765573371773 1104 70-71 0.14349590770704168 1104 72-73 0.25029386304221646 1104 74-75 0.2787012739961767 1104 76-77 0.22821667537977675 1104 78-79 0.19183156006706525 1104 80-81 0.18127262887272622 1104 82-83 0.16372942915079136 1104 84-85 0.2046351410890921 1104 86-87 0.157875967181468 1104 88-89 0.12065948024822859 1104 90-91 0.11143019764152484 1104 92-93 0.10984150278717664 1104 94-95 0.12759680901493198 1104 96-97 0.16138258241171854 1104 98-99 0.18206571252200732 1104 100-101 0.13541737929657316 1105 1 0.12662282897592547 1105 2 0.11612579269979051 1105 3 0.1242812460670848 1105 4 0.09857225997651398 1105 5 0.0924230132438808 1105 6 0.10275413142987588 1105 7 0.1351310122242282 1105 8 0.11870073698252526 1105 9 0.2271510155057186 1105 10-11 0.18939696276432727 1105 12-13 0.15559576434988998 1105 14-15 0.1966025940628171 1105 16-17 0.21903219283041153 1105 18-19 0.24007297487467127 1105 20-21 0.2242119193689689 1105 22-23 0.20083548435404452 1105 24-25 0.19156317821492763 1105 26-27 0.24252047510247365 1105 28-29 0.21077151700630736 1105 30-31 0.19670582427734473 1105 32-33 0.24530232074750558 1105 34-35 0.2652288275275225 1105 36-37 0.2121233874101307 1105 38-39 0.2317301216563692 1105 40-41 0.27320746294043374 1105 42-43 0.22239807932653832 1105 44-45 0.20040339269666418 1105 46-47 0.20569630480435563 1105 48-49 0.21351426275080598 1105 50-51 0.18068826265112392 1105 52-53 0.11530647784087478 1105 54-55 0.18211739792101866 1105 56-57 0.170076323015536 1105 58-59 0.20838449425703942 1105 60-61 0.1698946617997592 1105 62-63 0.1993096210353329 1105 64-65 0.27671134118036633 1105 66-67 0.2659607321145998 1105 68-69 0.3501424781368172 1105 70-71 0.24000058633644272 1105 72-73 0.28100162980291543 1105 74-75 0.2925032379100614 1105 76-77 0.2913112853616582 1105 78-79 0.2659762063260942 1105 80-81 0.23952383758337703 1105 82-83 0.22750851487474222 1105 84-85 0.26329730854622824 1105 86-87 0.23726425717305943 1105 88-89 0.24183106940571264 1105 90-91 0.16169755816726905 1105 92-93 0.17686373915073972 1105 94-95 0.17442589620607407 1105 96-97 0.11425016681432965 1105 98-99 0.11659295029810579 1105 100-101 0.038496743385021404 1106 1 0.10055327168545602 1106 2 0.08631520675537985 1106 3 0.1127954838319738 1106 4 0.05147654378119171 1106 5 0.09759074553013392 1106 6 0.14119156652744635 1106 7 0.120712391689338 1106 8 0.08826924816769832 1106 9 0.15490855882395493 1106 10-11 0.1682864742519783 1106 12-13 0.09366577325676673 1106 14-15 0.08522290592903659 1106 16-17 0.15359607634125894 1106 18-19 0.1077770002543943 1106 20-21 0.14414175852940048 1106 22-23 0.1701019770647605 1106 24-25 0.14433281342316917 1106 26-27 0.137457039723337 1106 28-29 0.07156142059177739 1106 30-31 0.10524911045578733 1106 32-33 0.19193185450931338 1106 34-35 0.18468499216121614 1106 36-37 0.1491402519377516 1106 38-39 0.12782496389031195 1106 40-41 0.1412508627199287 1106 42-43 0.07427981436987352 1106 44-45 0.15307117028395112 1106 46-47 0.13940612453414047 1106 48-49 0.1017226837242049 1106 50-51 0.12126070039008852 1106 52-53 0.05256186970871113 1106 54-55 0.09673680655441785 1106 56-57 0.1666822305484601 1106 58-59 0.17316053990932545 1106 60-61 0.14625707281808786 1106 62-63 0.07990098763495013 1106 64-65 0.10270646539579076 1106 66-67 0.11138351616249764 1106 68-69 0.19252336779327095 1106 70-71 0.12624053821978265 1106 72-73 0.1748067489585594 1106 74-75 0.12741705444506835 1106 76-77 0.10755975383840877 1106 78-79 0.09485167449479803 1106 80-81 0.06939293154886883 1106 82-83 0.07016044407131972 1106 84-85 0.09358407799744128 1106 86-87 0.14569324537109196 1106 88-89 0.14784150536886642 1106 90-91 0.10137739140373014 1106 92-93 0.19422072380275068 1106 94-95 0.13734811921816004 1106 96-97 0.16215373974132774 1106 98-99 0.026242452753418632 1106 100-101 0.012127432671537264 1107 1 0.039027817960260336 1107 2 0.08950259578140418 1107 3 0.07605104885594471 1107 4 0.06365540339074016 1107 5 0.06347783655683514 1107 6 0.06727600410188472 1107 7 0.10529717554861406 1107 8 0.10825317345883434 1107 9 0.11016854183537106 1107 10-11 0.09431237076481835 1107 12-13 0.049294640828655645 1107 14-15 0.0837063625165797 1107 16-17 0.1004170162240996 1107 18-19 0.07536708460740016 1107 20-21 0.10534223988407376 1107 22-23 0.054766563294450066 1107 24-25 0.05851733737274145 1107 26-27 0.08448405403201065 1107 28-29 0.13510544548200443 1107 30-31 0.17430257642422475 1107 32-33 0.18475528471033442 1107 34-35 0.12000730644189161 1107 36-37 0.06955205467508563 1107 38-39 0.08912002141178732 1107 40-41 0.04953851066674986 1107 42-43 0.07068446225908076 1107 44-45 0.11396430893393728 1107 46-47 0.14874282284895202 1107 48-49 0.15610914577762713 1107 50-51 0.1381461948578817 1107 52-53 0.17441702887526844 1107 54-55 0.25985869983730936 1107 56-57 0.2260081021342515 1107 58-59 0.23814287443854454 1107 60-61 0.216604801779539 1107 62-63 0.21136940410563199 1107 64-65 0.2370244939967634 1107 66-67 0.3123071043485126 1107 68-69 0.24326050606543248 1107 70-71 0.25287453885471933 1107 72-73 0.2604729354981572 1107 74-75 0.21994492346858863 1107 76-77 0.11021312937382532 1107 78-79 0.17402424471253397 1107 80-81 0.13148722332221752 1107 82-83 0.019921395009362186 1107 84-85 0.09169275073595173 1107 86-87 0.14834054259328155 1107 88-89 0.09329093738332261 1107 90-91 0.1282414216681076 1107 92-93 0.07487438202661423 1107 94-95 0.12838539487896128 1107 96-97 0.09581568561313247 1107 98-99 0.056073236946193816 1107 100-101 -0.03437275470759715 1108 1 0.01958004413859271 1108 2 0.049859253467744225 1108 3 0.06839425085586015 1108 4 0.05670249944762418 1108 5 0.05088447890104675 1108 6 -0.01033256226338608 1108 7 0.01752360521529539 1108 8 0.017026297100940724 1108 9 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-0.1990411147364597 2214 52-53 -0.18574242836948684 2214 54-55 -0.1525751542244933 2214 56-57 -0.1363723156192833 2214 58-59 -0.20794821236081162 2214 60-61 -0.2653894461621462 2214 62-63 -0.2570324120664864 2214 64-65 -0.22669024757987444 2214 66-67 -0.23206561496258615 2214 68-69 -0.21475170585856063 2214 70-71 -0.24005100471845253 2214 72-73 -0.17530763163057372 2214 74-75 -0.15130502421157388 2214 76-77 -0.14898518366389624 2214 78-79 -0.13594307091012325 2214 80-81 -0.2384577857676149 2214 82-83 -0.18425807263000138 2214 84-85 -0.14258164392019523 2214 86-87 -0.1184912446748001 2214 88-89 -0.10696951793683951 2214 90-91 -0.18992028852801468 2214 92-93 -0.15707532065185603 2214 94-95 -0.1905478694973617 2214 96-97 -0.13944394268951044 2214 98-99 -0.10042779013549463 2214 100-101 -0.17147732027176588 2215 1 -0.20641662989842047 2215 2 -0.24187750878173375 2215 3 -0.23323138729045922 2215 4 -0.13647864439290203 2215 5 -0.20311461235822037 2215 6 -0.16411858401909285 2215 7 -0.1706906279539524 2215 8 -0.20443703180456652 2215 9 -0.23738768422865064 2215 10-11 -0.21202131663101653 2215 12-13 -0.27113140458563123 2215 14-15 -0.3726426694839944 2215 16-17 -0.3476577218094974 2215 18-19 -0.3010449649460085 2215 20-21 -0.3062456800739639 2215 22-23 -0.30048554285625073 2215 24-25 -0.24845986653748042 2215 26-27 -0.24959385277401935 2215 28-29 -0.31264389917893 2215 30-31 -0.36544286995537334 2215 32-33 -0.30735770525006956 2215 34-35 -0.24781547069586907 2215 36-37 -0.29445906993124993 2215 38-39 -0.30029442056422084 2215 40-41 -0.3777977027861539 2215 42-43 -0.3233136918360344 2215 44-45 -0.34486285352324586 2215 46-47 -0.3589308572020613 2215 48-49 -0.3613559330047167 2215 50-51 -0.39519858815319964 2215 52-53 -0.36509703548000516 2215 54-55 -0.34851899912547424 2215 56-57 -0.3182499563514085 2215 58-59 -0.3474344220274901 2215 60-61 -0.3699323972740842 2215 62-63 -0.4093947617034317 2215 64-65 -0.40844575831483354 2215 66-67 -0.29892158833428795 2215 68-69 -0.14884888546760067 2215 70-71 -0.19707529730077766 2215 72-73 -0.28160382160808695 2215 74-75 -0.2877684695635949 2215 76-77 -0.3982261411819721 2215 78-79 -0.375344232033477 2215 80-81 -0.3559311591638661 2215 82-83 -0.3907948384893132 2215 84-85 -0.3642721495176815 2215 86-87 -0.461390010819386 2215 88-89 -0.39862607829935115 2215 90-91 -0.3904942029664795 2215 92-93 -0.4003742853273309 2215 94-95 -0.42613018821332815 2215 96-97 -0.4029434783968675 2215 98-99 -0.3646910846000537 2215 100-101 -0.4623266682421949 2216 1 -0.3760851032029393 2216 2 -0.3221257747223021 2216 3 -0.34964412637975784 2216 4 -0.2346962734007505 2216 5 -0.314635882538731 2216 6 -0.3013821511263117 2216 7 -0.36578806548900644 2216 8 -0.3614018471282847 2216 9 -0.4966863720126824 2216 10-11 -0.5319751453164443 2216 12-13 -0.48597589202410774 2216 14-15 -0.666710805094425 2216 16-17 -0.6523652844195453 2216 18-19 -0.5914096985735142 2216 20-21 -0.5886878444546539 2216 22-23 -0.5419270202589104 2216 24-25 -0.5246925463861487 2216 26-27 -0.44126107955419513 2216 28-29 -0.531215729316969 2216 30-31 -0.5587513238501884 2216 32-33 -0.5138472134496155 2216 34-35 -0.6314839716988274 2216 36-37 -0.5954191777490792 2216 38-39 -0.5588811024546345 2216 40-41 -0.5679971367548191 2216 42-43 -0.5215218272744124 2216 44-45 -0.45710442494934256 2216 46-47 -0.428767723788134 2216 48-49 -0.5629885835289627 2216 50-51 -0.5395711300938828 2216 52-53 -0.6224166437162921 2216 54-55 -0.5888127519549897 2216 56-57 -0.4979614421193759 2216 58-59 -0.502440801649449 2216 60-61 -0.5154641969268212 2216 62-63 -0.5501847514682439 2216 64-65 -0.5220541012362716 2216 66-67 -0.44015257886170644 2216 68-69 -0.420139449856876 2216 70-71 -0.5272105124831583 2216 72-73 -0.5196366774411132 2216 74-75 -0.33562697836843824 2216 76-77 -0.31537196486693375 2216 78-79 -0.34198878721496584 2216 80-81 -0.446595905657432 2216 82-83 -0.4600740730821542 2216 84-85 -0.4541057182877566 2216 86-87 -0.4469648309709555 2216 88-89 -0.34102591936891713 2216 90-91 -0.45871731636001556 2216 92-93 -0.4089912298906526 2216 94-95 -0.41289209450128084 2216 96-97 -0.43736845641536704 2216 98-99 -0.46614361727263187 2216 100-101 -0.5543202781269656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 18.0 13 144.0 14 570.0 15 1579.0 16 2952.0 17 5493.0 18 11654.0 19 22173.0 20 19450.0 21 16784.0 22 16244.0 23 17220.0 24 19111.0 25 23495.0 26 29662.0 27 38150.0 28 48021.0 29 59296.0 30 74018.0 31 98010.0 32 136159.0 33 202458.0 34 333979.0 35 573965.0 36 718619.0 37 1206155.0 38 1176960.0 39 208285.0 40 114.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.704110743585392 33.78992866930861 24.737163909761936 20.768796677344067 2 20.664812128191578 32.18817097427292 29.2469201132325 17.900096784303003 3 20.321897715313458 32.255157251768416 26.85060953560528 20.572335497312842 4 22.388651615633925 31.020080470476834 26.119253753108733 20.472014160780503 5 22.72036212900174 31.026818618154113 23.87329674051492 22.37952251232923 6 22.98239900978869 29.8747534450509 24.044971306556477 23.097876238603934 7 23.08789745685313 30.691057312194385 24.03406380650411 22.18698142444837 8 23.465648686021684 29.810415002713043 24.3596487310744 22.364287580190872 9 22.07270955342877 29.431893134953835 25.192373128188024 23.303024183429375 10-11 22.748974556675332 29.782504053756586 24.564826315845632 22.90369507372245 12-13 22.278391017278505 29.953170861641127 24.902573498173584 22.865864622906777 14-15 22.02826939470093 30.0561993132227 25.20495034518681 22.710580946889564 16-17 22.101351621048153 30.331524769707503 25.13310311658102 22.434020492663322 18-19 21.595921385378972 30.379294482346253 25.74574103618879 22.279043096085985 20-21 21.03611172915887 30.43161886665542 26.235432460641118 22.296836943544598 22-23 20.82636959273529 30.145889789196755 26.933028344877762 22.094712273190193 24-25 20.76121111189712 29.66457658942233 28.059227725284337 21.514984573396212 26-27 20.764758025410522 29.691954019354487 28.33466186157039 21.208626093664602 28-29 20.873931825753477 29.820670423957928 28.177817148407996 21.127580601880595 30-31 20.87395158571734 29.678052884776886 28.291130661180247 21.156864868325528 32-33 20.84502299862194 29.731730826610665 28.30566411460147 21.11758206016593 34-35 20.90439181004826 29.85983467233435 28.174210955003005 21.061562562614387 36-37 20.875265623314228 29.802590057023302 28.139719938080177 21.18242438158229 38-39 20.866897278618257 29.91443145248776 27.985829339515305 21.23284192937868 40-41 21.049094025416846 30.110588613755546 27.50380478104182 21.336512579785794 42-43 21.04797758745859 30.07221476393364 27.530599292040016 21.349208356567758 44-45 21.045645911722758 30.304898218402137 27.30555306360456 21.34390280627055 46-47 21.26403303233639 30.495315110167727 26.759990341329665 21.48066151616622 48-49 21.180448385195994 30.517070830380867 26.625385467495054 21.677095316928085 50-51 21.17498475518788 30.53934030965444 26.595231762640154 21.690443172517526 52-53 21.28977038526792 30.661269166670944 26.32297898053604 21.725981467525095 54-55 21.26303515416131 30.52937140788557 26.43630237329022 21.7712910646629 56-57 21.1784427488639 30.516547191338496 26.594708123597783 21.71030193619982 58-59 21.312484463728413 30.66845191353514 26.349220212546076 21.66984341019037 60-61 21.254044370603655 30.63098702205093 26.359653473465727 21.755315133879684 62-63 21.252117774127015 30.730646399793866 26.39742464438981 21.61981118168931 64-65 21.257630804044787 30.797682077199017 26.276098466271126 21.66858865248507 66-67 21.28850574758069 30.659065930700223 26.340782707976583 21.711645613742505 68-69 21.246683784064697 30.766826893626977 26.29483091201323 21.6916584102951 70-71 21.28310139746416 30.887382433155008 26.20240368104415 21.62711248833668 72-73 21.25118337506968 30.848846719361234 26.24172131450419 21.6582485910649 74-75 21.210700889870214 30.945229727363877 26.26331376965178 21.580755613114135 76-77 21.271690018333295 31.030622411197733 26.107427414736744 21.590260155732228 78-79 21.209515292038432 30.98872140782629 26.19074530236499 21.611017997770286 80-81 21.06650745155003 31.047303428990514 26.33010111233548 21.556088007123975 82-83 21.10337765726737 31.217537821371245 26.09221615125347 21.58686837010791 84-85 21.05415257616577 31.112329861771148 26.222450164383137 21.611067397679943 86-87 21.00334970907405 31.246272776816348 26.249511434893485 21.500866079216113 88-89 21.05132690133336 31.394699745373106 25.972506381480333 21.5814669718132 90-91 20.969550290886428 31.292688931930478 26.068065566721693 21.6696952104614 92-93 21.011530334113324 31.406456923871577 26.07794554865318 21.504067193361916 94-95 21.01420780921676 31.486366217733462 25.971617183106492 21.527808789943283 96-97 20.87926701599648 31.535202968420812 25.973682099330176 21.61184791625253 98-99 20.90708247669274 31.57875028763265 26.04978136954539 21.464385866129216 100-101 20.847819033508554 31.735410922280504 25.746472154851723 21.67029788935922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 221205.0 1 119208.0 2 11579.5 3 4870.5 4 3445.0 5 2789.5 6 2407.5 7 2224.5 8 2083.0 9 2009.0 10 1982.5 11 2019.0 12 2192.5 13 2670.5 14 3094.0 15 3763.5 16 12058.5 17 12022.5 18 3768.5 19 3447.5 20 4165.0 21 5498.0 22 7448.5 23 10009.0 24 13214.5 25 17134.5 26 21765.5 27 27596.0 28 34276.0 29 40818.5 30 47739.0 31 56542.0 32 67409.0 33 77895.5 34 87452.0 35 100056.5 36 113343.0 37 127178.5 38 140622.0 39 151659.5 40 162962.0 41 174112.0 42 187041.5 43 201310.5 44 210536.0 45 210991.0 46 207682.5 47 203387.0 48 200526.0 49 197020.0 50 189749.0 51 177986.5 52 158682.5 53 138376.5 54 123526.0 55 113079.5 56 102317.0 57 90570.0 58 82041.0 59 75279.0 60 67145.5 61 59347.5 62 51923.5 63 44750.5 64 38433.0 65 32089.0 66 26316.5 67 20735.5 68 15609.0 69 11634.0 70 8364.0 71 5854.5 72 4094.0 73 2795.5 74 1862.5 75 1124.0 76 626.5 77 408.5 78 252.0 79 94.5 80 27.0 81 8.0 82 2.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17062728795681578 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.9759963862978088E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015709171271067579 82-83 8.101585183821016E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.829189339578535E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5060738.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.478800644139056 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39648653568462 41.26358131720767 2 8.857096948941196 8.764770684449473 3 2.40957837046989 3.576691434867274 4 1.1235564426125748 2.223689009458626 5 0.6552356218327918 1.6210136353801596 6 0.5044334209820294 1.4975256405006543 7 0.34937904607331316 1.2100799318930657 8 0.2855067655741618 1.1301225869117517 9 0.22309482088759855 0.993461775069366 >10 1.9027378475057242 19.224236083700426 >50 0.22560982034894472 7.696604749230902 >100 0.06628262210781655 4.646427051445006 >500 5.609728229976415E-4 0.19675222751369398 >1k 2.804862813073043E-4 0.29455328585326856 >5k 4.006946875812834E-5 0.17252491710933116 >10k+ 1.2020840627438501E-4 5.4879656694093 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 253917 5.017390744195807 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12095 0.23899676292271996 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 10796 0.21332856986471144 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8702 0.17195120553563534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002035276277886743 0.0 0.0 0.0 0.0 2 0.002074796205612699 0.0 0.0 0.0 0.0 3 0.002074796205612699 0.0 0.0 0.0 0.0 4 0.0021340760972016334 0.0 0.0 0.0 0.0 5 0.002193355988790568 0.0 0.0 0.0 0.0 6 0.002193355988790568 0.0 0.0 0.0 0.0 7 0.0022131159526535458 0.0 0.0 0.0 0.0 8 0.002311915771968436 0.0 0.0 0.0 0.0 9 0.002568795302187151 0.0 0.0 0.0 0.0 10-11 0.0026379551757075743 0.0 0.0 0.0 0.0 12-13 0.002746634976953954 0.0 0.0 0.0 0.0 14-15 0.002924474651720757 0.0 0.0 0.0 0.0 16-17 0.0031615942180764937 0.0 0.0 0.0 0.0 18-19 0.0034975136037471216 0.0 0.0 0.0 0.0 20-21 0.0037741530978288146 0.0 0.0 0.0 0.0 22-23 0.004110072483499442 0.0 0.0 0.0 0.0 24-25 0.004722631363251763 0.0 0.0 0.0 0.0 26-27 0.005107950658579836 0.0 0.0 0.0 0.0 28-29 0.005532789881633865 0.0 0.0 0.0 0.0 30-31 0.005957629104687893 0.0 0.0 0.0 0.0 32-33 0.00651090809285128 1.9759963862978088E-5 0.0 0.0 0.0 34-35 0.0071333469545350895 1.9759963862978088E-5 0.0 0.0 0.0 36-37 0.007528546231794652 1.9759963862978088E-5 0.0 0.0 0.0 38-39 0.00786446561746528 1.9759963862978088E-5 0.0 0.0 0.0 40-41 0.00827942485858782 1.9759963862978088E-5 0.0 0.0 0.0 42-43 0.008733904027436314 1.9759963862978088E-5 0.0 0.0 0.0 44-45 0.00917850321435332 1.9759963862978088E-5 0.0 0.0 0.0 46-47 0.009435382744572037 1.9759963862978088E-5 0.0 1.9759963862978088E-5 0.0 48-49 0.009879981931489045 1.9759963862978088E-5 0.0 1.9759963862978088E-5 0.0 50-51 0.010314701136474562 1.9759963862978088E-5 0.0 1.9759963862978088E-5 0.0 52-53 0.011717658570746005 1.9759963862978088E-5 0.0 1.9759963862978088E-5 0.0 54-55 0.01364425504738637 5.927989158893426E-5 0.0 1.9759963862978088E-5 0.0 56-57 0.015363371903465463 5.927989158893426E-5 0.0 1.9759963862978088E-5 0.0 58-59 0.01724056847044838 6.91598735204233E-5 0.0 1.9759963862978088E-5 0.0 60-61 0.019345004621855545 7.903985545191235E-5 0.0 1.9759963862978088E-5 0.0 62-63 0.02157788053837207 7.903985545191235E-5 0.0 1.9759963862978088E-5 0.0 64-65 0.024601155009407717 7.903985545191235E-5 0.0 1.9759963862978088E-5 0.0 66-67 0.027822029119073145 7.903985545191235E-5 0.0 1.9759963862978088E-5 0.0 68-69 0.031606062198833454 9.879981931489044E-5 0.0 1.9759963862978088E-5 0.0 70-71 0.03556793495336056 9.879981931489044E-5 0.0 1.9759963862978088E-5 0.0 72-73 0.04074504548546082 9.879981931489044E-5 0.0 1.9759963862978088E-5 0.0 74-75 0.046732314535943176 9.879981931489044E-5 0.0 1.9759963862978088E-5 0.0 76-77 0.05418182091228592 1.0867980124637947E-4 0.0 1.9759963862978088E-5 0.0 78-79 0.06371600347617284 1.1855978317786852E-4 0.0 1.9759963862978088E-5 0.0 80-81 0.07563126168554862 1.2843976510935756E-4 0.0 1.9759963862978088E-5 0.0 82-83 0.09041171465505624 1.383197470408466E-4 0.0 1.9759963862978088E-5 0.0 84-85 0.10798820251117525 1.383197470408466E-4 0.0 1.9759963862978088E-5 0.0 86-87 0.12951668313988987 1.383197470408466E-4 0.0 1.9759963862978088E-5 0.0 88-89 0.15639023399354007 1.383197470408466E-4 0.0 1.9759963862978088E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2860 0.0 88.175896 1 TCGACGG 3250 0.0 77.459854 2 CGACGGC 5055 0.0 49.707127 3 ATATCTC 4830 0.0 48.763863 1 GACGGCG 5175 0.0 48.5545 4 TATCTCG 4885 0.0 47.839287 2 ACGGCGC 5310 0.0 47.588417 5 ATCTCGA 8470 0.0 46.79523 1 TCTCGAG 9535 0.0 41.4464 2 CGGCGCG 7010 0.0 36.11548 6 CTCGAGG 11300 0.0 34.888622 3 TCGAGGG 11670 0.0 33.94528 4 AGGGCGC 13010 0.0 30.55852 7 GGGCGCG 13155 0.0 30.149477 8 CGAGGGC 13295 0.0 29.90345 5 GAGGGCG 14535 0.0 27.319666 6 CGCGCCG 20145 0.0 20.442593 9 GGCGCGC 20555 0.0 20.10416 7 GCGCGCC 20585 0.0 20.097937 8 ATCCATA 8265 0.0 18.217995 16-17 >>END_MODULE