FastQCFastQC Report
Thu 26 May 2016
SRR1051295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7075247
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT5118027.233697989624956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1111841.571450438408723No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA235660.3330767109614689No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA200170.2829159179884462No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT194730.2752271404800426No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC178410.2521608079548318No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT173260.2448819101297806No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT156980.2218721127333081No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC150410.21258621783804862No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135040.19086259462037156No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC123120.17401512625636958No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT117330.16583166637150618No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG114000.16112511690404588No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA102120.14433418366878215No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG87500.1236705941149475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1068150.094.0779951
TCGACGG1091950.092.031832
ATATCTC1000600.091.1106341
TATCTCG1007250.090.334632
CGACGGC1124050.089.306443
GACGGCG1131600.088.7189944
ACGGCGC1136100.088.338325
CGGCGCG1154850.086.8752756
ATCTCGA1051600.086.461643
TCTCGAG1048600.085.191524
CTCGAGG1077550.082.8983155
TCGAGGG1090750.081.943016
AGGGCGC1124000.079.890869
CGAGGGC1120250.079.853017
GAGGGCG1133950.079.089328
CTCGACG21300.071.1373755
TCTCGAC24150.063.1356474
AATTCGA18850.047.625241
GGCGCGC2289100.044.816257
CGCGCCG2290050.044.7852139
Produced by FastQC (version 0.11.5)