##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051295_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7075247 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23129058250546 33.0 31.0 34.0 31.0 34.0 2 32.50987972575374 34.0 31.0 34.0 31.0 34.0 3 32.56656523793445 34.0 31.0 34.0 31.0 34.0 4 36.104222368491165 37.0 37.0 37.0 35.0 37.0 5 36.018431299995605 37.0 37.0 37.0 35.0 37.0 6 36.0244993567009 37.0 36.0 37.0 35.0 37.0 7 35.965695331908556 37.0 35.0 37.0 35.0 37.0 8 36.02306294041749 37.0 36.0 37.0 35.0 37.0 9 37.671035795640776 39.0 38.0 39.0 35.0 39.0 10-11 37.6789220220863 39.0 38.0 39.0 35.0 39.0 12-13 37.66325479520361 39.0 38.0 39.0 35.0 39.0 14-15 39.05427859974358 41.0 39.0 41.0 36.0 41.0 16-17 38.98261057175813 40.5 39.0 41.0 35.5 41.0 18-19 38.85529911535244 40.5 38.5 41.0 35.0 41.0 20-21 38.895126841508144 40.5 39.0 41.0 35.0 41.0 22-23 38.8667659941766 40.5 38.5 41.0 35.0 41.0 24-25 38.830690504515246 40.5 38.5 41.0 35.0 41.0 26-27 38.879685189789136 41.0 38.5 41.0 35.0 41.0 28-29 38.56077045790769 40.0 38.0 41.0 34.5 41.0 30-31 37.84281319083277 39.5 36.0 40.5 34.5 41.0 32-33 37.33329126177503 38.0 35.0 41.0 34.5 41.0 34-35 37.00743783220572 38.0 35.0 40.5 34.0 41.0 36-37 36.88547156021549 38.0 35.0 40.0 33.0 41.0 38-39 36.582966220119246 38.0 35.0 40.5 32.5 41.0 40-41 35.2489871378342 38.0 35.0 40.5 24.5 41.0 42-43 35.197464696285515 37.0 34.0 40.0 25.0 41.0 44-45 35.1111873550139 37.5 34.0 40.0 24.0 41.0 46-47 35.19806191925173 38.0 34.0 40.0 24.0 41.0 48-49 34.59134034472578 37.5 33.0 40.0 23.0 41.0 50-51 34.64875275732423 37.0 33.0 40.0 23.5 41.0 52-53 34.615666421257096 37.0 33.0 40.0 24.0 41.0 54-55 34.24500098724469 36.5 33.0 40.0 24.0 41.0 56-57 33.87892182421335 36.0 33.0 40.0 23.0 41.0 58-59 33.520003188581256 35.5 33.0 40.0 21.0 41.0 60-61 33.19045299761267 35.0 32.5 40.0 19.0 41.0 62-63 32.84178495817885 35.0 32.0 39.5 18.0 41.0 64-65 32.483859644758695 35.0 32.0 39.0 18.0 41.0 66-67 32.09965178600832 35.0 32.0 39.0 17.5 41.0 68-69 31.76411085012297 35.0 31.0 38.0 14.0 40.0 70-71 31.388936880931507 35.0 31.0 37.0 10.0 40.0 72-73 31.043350006013924 35.0 31.0 37.0 7.5 39.5 74-75 30.724063414323204 35.0 31.0 36.0 7.0 39.0 76-77 30.42642765687191 35.0 31.0 36.0 7.0 39.0 78-79 30.182597582812303 35.0 31.0 35.0 2.0 37.5 80-81 29.92953970370222 35.0 30.0 35.0 2.0 37.0 82-83 29.73637181853863 35.0 30.0 35.0 2.0 36.5 84-85 29.53921064522553 35.0 30.0 35.0 2.0 36.0 86-87 29.33858266714929 34.5 30.0 35.0 2.0 36.0 88-89 29.13955526923654 34.0 30.0 35.0 2.0 36.0 90-91 28.93467669750611 34.0 30.0 35.0 2.0 35.0 92-93 28.69279220923312 34.0 30.0 35.0 2.0 35.0 94-95 28.462354529813588 34.0 29.0 35.0 2.0 35.0 96-97 28.189479180020147 34.0 29.0 35.0 2.0 35.0 98-99 27.86633491381997 34.0 29.0 35.0 2.0 35.0 100-101 26.3405760957886 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.274713663915513 1101 2 0.2389845293612467 1101 3 0.24650970790710858 1101 4 0.20471108510795233 1101 5 0.1996538690492855 1101 6 0.22777518149695908 1101 7 0.244116768650251 1101 8 0.2031485149330905 1101 9 0.2888156327627911 1101 10-11 0.27618842816010414 1101 12-13 0.28886430029240984 1101 14-15 0.3497248284820884 1101 16-17 0.3787385212055412 1101 18-19 0.3869002144286071 1101 20-21 0.39117624956322317 1101 22-23 0.39993293265768415 1101 24-25 0.399865856723693 1101 26-27 0.3429530831393208 1101 28-29 0.382871561627546 1101 30-31 0.32545113455682184 1101 32-33 0.3177729813094814 1101 34-35 0.32307291453916775 1101 36-37 0.3297253954563928 1101 38-39 0.29194285470923376 1101 40-41 0.0452548349544486 1101 42-43 0.10113473018901686 1101 44-45 0.08627213442814963 1101 46-47 0.09709154762707328 1101 48-49 0.1799681813410814 1101 50-51 0.2715954771293809 1101 52-53 0.06806020994543616 1101 54-55 -0.05558480966830359 1101 56-57 -0.14844069379788039 1101 58-59 -0.05417907843204972 1101 60-61 -0.03005027710590724 1101 62-63 -0.11174445599413474 1101 64-65 -0.15630974800003372 1101 66-67 -0.13825298226453242 1101 68-69 -0.1832360402098594 1101 70-71 -0.17254228718278242 1101 72-73 -0.2287916329235813 1101 74-75 -0.25020512009899676 1101 76-77 -0.2601127839390216 1101 78-79 -0.27282391492929037 1101 80-81 -0.30174829519541646 1101 82-83 -0.24974249429068252 1101 84-85 -0.2539243697661675 1101 86-87 -0.25695644631142756 1101 88-89 -0.23053729252708166 1101 90-91 -0.23532728071632647 1101 92-93 -0.2396908800179034 1101 94-95 -0.158685964473392 1101 96-97 -0.17204254529649177 1101 98-99 -0.18126324385018222 1101 100-101 -0.07052825854828981 1102 1 0.20997751140189536 1102 2 0.24039082565447956 1102 3 0.24224558752335668 1102 4 0.19399695979002018 1102 5 0.1947098526040989 1102 6 0.21141343437006554 1102 7 0.18272974599297953 1102 8 0.14663510670513347 1102 9 0.23093158175311856 1102 10-11 0.21930211455710236 1102 12-13 0.21317329055442258 1102 14-15 0.3511918639542557 1102 16-17 0.35637046304886866 1102 18-19 0.3515844779792161 1102 20-21 0.35196083251724986 1102 22-23 0.3875027676300036 1102 24-25 0.33200439393874603 1102 26-27 0.27006056767302766 1102 28-29 0.3177644410198397 1102 30-31 0.29282121427324626 1102 32-33 0.31053119880283475 1102 34-35 0.33778891555986945 1102 36-37 0.3528697565056831 1102 38-39 0.30880641888312255 1102 40-41 0.02295353521708421 1102 42-43 0.0352805025969829 1102 44-45 -0.07465498621198918 1102 46-47 0.04377695793488101 1102 48-49 0.1434993102127038 1102 50-51 0.1997838183217624 1102 52-53 0.13348686407323385 1102 54-55 0.07185811577188872 1102 56-57 0.033730084297495466 1102 58-59 -0.015442961666209953 1102 60-61 -0.08523058620883717 1102 62-63 -0.05691030270545383 1102 64-65 -0.16220063762094838 1102 66-67 -0.04550267698488142 1102 68-69 -0.08835859100122079 1102 70-71 -0.1433304422755164 1102 72-73 -0.16253092984689488 1102 74-75 -0.16997634314869003 1102 76-77 -0.13360092704418491 1102 78-79 -0.19939355181816865 1102 80-81 -0.1922771670545842 1102 82-83 -0.15824815512936752 1102 84-85 -0.16501358567436597 1102 86-87 -0.2296202853209124 1102 88-89 -0.18491536274032683 1102 90-91 -0.09420438949143772 1102 92-93 -0.09858615964004258 1102 94-95 -0.10513515376985438 1102 96-97 -0.057371128134079186 1102 98-99 -0.014040403143546598 1102 100-101 0.002846757718273807 1103 1 0.15230947300714348 1103 2 0.2108425221085639 1103 3 0.23558422799938938 1103 4 0.16622976922617738 1103 5 0.1960107878701578 1103 6 0.25084381594651006 1103 7 0.18109557615711225 1103 8 0.16084806988106948 1103 9 0.2703502544483527 1103 10-11 0.2423759510648793 1103 12-13 0.24921227088711362 1103 14-15 0.2874592829501452 1103 16-17 0.34035065006951726 1103 18-19 0.4124484067516576 1103 20-21 0.3249747057335668 1103 22-23 0.3244435372179453 1103 24-25 0.2828705633953916 1103 26-27 0.2854817558183598 1103 28-29 0.2978715582571354 1103 30-31 0.27293501891924166 1103 32-33 0.26119899879833497 1103 34-35 0.2340092584167337 1103 36-37 0.2941610049206105 1103 38-39 0.23263074734849454 1103 40-41 -0.03804473805895725 1103 42-43 0.006165967191854804 1103 44-45 -0.04153662869484265 1103 46-47 -0.04773244482679928 1103 48-49 0.05571304297544799 1103 50-51 0.06258297716027528 1103 52-53 -0.004888201814075899 1103 54-55 -0.01500785422675932 1103 56-57 0.0020549481436518136 1103 58-59 0.12384813249815352 1103 60-61 0.0814345849014515 1103 62-63 0.05766440810648277 1103 64-65 -0.026547380207354365 1103 66-67 -0.0453431493944052 1103 68-69 -0.06805035058595266 1103 70-71 -0.06536464655841101 1103 72-73 -0.16350855516878937 1103 74-75 -0.16580064090782543 1103 76-77 -0.14632882167508043 1103 78-79 -0.15813558615837664 1103 80-81 -0.17274275400906092 1103 82-83 -0.16308829141733838 1103 84-85 -0.17183047832811837 1103 86-87 -0.17899541347258108 1103 88-89 -0.19583162746675242 1103 90-91 -0.18065126294659706 1103 92-93 -0.20359199789455928 1103 94-95 -0.16541718806198347 1103 96-97 -0.1734107932044182 1103 98-99 -0.15776415127815113 1103 100-101 -0.04352795116647101 1104 1 0.12945136159051174 1104 2 0.14036105039239288 1104 3 0.17174961331765815 1104 4 0.11353338532108381 1104 5 0.1301589667092813 1104 6 0.15526139183344156 1104 7 0.12624772668799267 1104 8 0.15202550140880078 1104 9 0.19302525232090773 1104 10-11 0.16581544728640552 1104 12-13 0.20356340080343216 1104 14-15 0.26867828680465067 1104 16-17 0.21321572275831358 1104 18-19 0.2754607263898379 1104 20-21 0.2834987067770598 1104 22-23 0.3054262205302791 1104 24-25 0.30105364345838836 1104 26-27 0.29310356761848055 1104 28-29 0.3073413855366667 1104 30-31 0.29714676993727096 1104 32-33 0.2803155868542433 1104 34-35 0.29821411791482433 1104 36-37 0.3266158089656841 1104 38-39 0.3043932605491051 1104 40-41 0.1293691981568088 1104 42-43 0.050454222451520536 1104 44-45 0.1359196192213048 1104 46-47 0.21157552938719704 1104 48-49 0.2181528502919008 1104 50-51 0.2516150952703242 1104 52-53 0.20444916598801655 1104 54-55 0.1530500205903138 1104 56-57 0.16534569076770822 1104 58-59 0.10964434718435001 1104 60-61 0.11152449552096044 1104 62-63 0.15713865767715873 1104 64-65 0.12948341121535378 1104 66-67 0.05888811829650109 1104 68-69 0.05085385589179836 1104 70-71 0.05845877986624615 1104 72-73 0.035599718421760684 1104 74-75 0.052456069605089084 1104 76-77 0.07567303931431013 1104 78-79 0.019655737501718562 1104 80-81 0.018974880609246014 1104 82-83 0.01976224490752898 1104 84-85 0.0665656808352999 1104 86-87 0.07813041814833355 1104 88-89 0.0025565938933311827 1104 90-91 0.04547193622530443 1104 92-93 0.1431803154938187 1104 94-95 0.10852742588037145 1104 96-97 0.16267193572587502 1104 98-99 0.15546850169197057 1104 100-101 0.19872787767830502 1105 1 0.04527238416176971 1105 2 0.14635872553471785 1105 3 0.1434267143849013 1105 4 0.11571422080536564 1105 5 0.08869709803628467 1105 6 0.15685254639070934 1105 7 0.15665782608938628 1105 8 0.1678739829289242 1105 9 0.26222084241371846 1105 10-11 0.21551502350329343 1105 12-13 0.20125163189793938 1105 14-15 0.2468091790374345 1105 16-17 0.27478230155634975 1105 18-19 0.32917006426305306 1105 20-21 0.28945764191902157 1105 22-23 0.2861291009740228 1105 24-25 0.2771593237996086 1105 26-27 0.25084600725455886 1105 28-29 0.2827728800357292 1105 30-31 0.275814072736253 1105 32-33 0.29805402206027765 1105 34-35 0.2772707428438963 1105 36-37 0.23925733696465556 1105 38-39 0.27204870108216994 1105 40-41 0.07016582802849314 1105 42-43 0.08807577406519584 1105 44-45 0.09400847637985521 1105 46-47 0.11724925758021243 1105 48-49 0.19101727630833665 1105 50-51 0.18081851116356518 1105 52-53 0.14411743900319607 1105 54-55 0.05098705264406078 1105 56-57 0.08685034963603044 1105 58-59 0.08722766221171696 1105 60-61 -0.011177602342733906 1105 62-63 -0.044364628243720006 1105 64-65 0.01721859711243212 1105 66-67 -0.012713928310489564 1105 68-69 -0.03217534670032407 1105 70-71 0.0015209103145572556 1105 72-73 -0.06527354479972303 1105 74-75 -0.10666169725012864 1105 76-77 -0.0964527638599435 1105 78-79 -0.10253136777589233 1105 80-81 -0.16729225835134187 1105 82-83 -0.14809577402083818 1105 84-85 -0.097153725319167 1105 86-87 -0.16593311728286153 1105 88-89 -0.11989871666738239 1105 90-91 -0.13054294652498655 1105 92-93 -0.03582901139581196 1105 94-95 -0.030639789965267283 1105 96-97 0.04049812515601303 1105 98-99 0.02229719903943561 1105 100-101 -0.001525316712747582 1106 1 0.049188403262178326 1106 2 0.08804006576463053 1106 3 0.08059071718049893 1106 4 0.05345572717050118 1106 5 0.08598995067702475 1106 6 0.08867793289379478 1106 7 0.08826087595139143 1106 8 0.06371835121058922 1106 9 0.12978825981418396 1106 10-11 0.12303549562519578 1106 12-13 0.0880871946124131 1106 14-15 0.10213749584632836 1106 16-17 0.11489903141938385 1106 18-19 0.14429288627511028 1106 20-21 0.12023690281449717 1106 22-23 0.1167458747862824 1106 24-25 0.16146026667133384 1106 26-27 0.1347780535305958 1106 28-29 0.08564246981435275 1106 30-31 0.13470590368251578 1106 32-33 0.15438580156807546 1106 34-35 0.19381325889786183 1106 36-37 0.19368306884435071 1106 38-39 0.12566601729221816 1106 40-41 -0.041313835270401 1106 42-43 -0.002389333821227524 1106 44-45 -0.030667835772568708 1106 46-47 -0.026222307984170357 1106 48-49 0.12660739652198316 1106 50-51 0.17343993609041775 1106 52-53 0.11404124068998556 1106 54-55 0.026697591954679467 1106 56-57 0.01077434391334009 1106 58-59 -0.026101772265349155 1106 60-61 0.012021640587157378 1106 62-63 0.03824469813967113 1106 64-65 0.01673544145784689 1106 66-67 -0.04724469348440863 1106 68-69 -0.010912808664592433 1106 70-71 -0.026509442522705484 1106 72-73 0.0017977197660528077 1106 74-75 0.01936143540793367 1106 76-77 -0.024552068747524913 1106 78-79 -0.01570002132877235 1106 80-81 6.705770384378695E-4 1106 82-83 0.05582278198619761 1106 84-85 0.05742149646364325 1106 86-87 0.03786222902990488 1106 88-89 -0.013710763686621164 1106 90-91 -0.04494174967675235 1106 92-93 0.004844694531687566 1106 94-95 0.0021583215757239316 1106 96-97 0.024348240067162408 1106 98-99 0.05278980735105776 1106 100-101 0.11020777819900474 1107 1 0.08748592349446938 1107 2 0.037133654311659825 1107 3 0.05157177795813794 1107 4 0.057181382334398734 1107 5 0.08969237723702861 1107 6 0.0889225775192628 1107 7 0.03465209684075887 1107 8 0.1050978502229114 1107 9 0.07904734783669198 1107 10-11 0.09588143942198002 1107 12-13 0.11008408533743363 1107 14-15 0.08400299662171307 1107 16-17 0.12858858929821793 1107 18-19 0.08960942074672573 1107 20-21 0.06993379463170868 1107 22-23 0.15340851325762372 1107 24-25 0.12398692482626927 1107 26-27 0.1446607643128175 1107 28-29 0.11607284097507176 1107 30-31 0.12967951160933922 1107 32-33 0.15915332539041316 1107 34-35 0.14945461354642475 1107 36-37 0.07250556106342287 1107 38-39 0.11029201348906525 1107 40-41 0.011786744567132246 1107 42-43 0.010619372387594694 1107 44-45 0.1443404037178695 1107 46-47 0.0396047816810281 1107 48-49 0.11553612810156721 1107 50-51 0.09208641572713816 1107 52-53 0.14012215962601715 1107 54-55 0.10218395556123028 1107 56-57 0.056737320738207586 1107 58-59 0.04844467236748784 1107 60-61 0.13508530408035568 1107 62-63 0.00724709122245315 1107 64-65 0.0028640011872980153 1107 66-67 0.0369795672968678 1107 68-69 -0.05484382355362527 1107 70-71 0.029454975791079363 1107 72-73 -0.0976366940198865 1107 74-75 -0.07580709373869254 1107 76-77 -0.0468434365500201 1107 78-79 -0.005506134664596374 1107 80-81 -0.0074939094137427276 1107 82-83 7.169208954493911E-5 1107 84-85 0.06089696813974399 1107 86-87 0.08450665468337348 1107 88-89 0.04277609916181646 1107 90-91 0.009749881346323264 1107 92-93 -0.021570382237722896 1107 94-95 0.04081550869876338 1107 96-97 0.046204486961396896 1107 98-99 0.05028387025318182 1107 100-101 0.09735602517417519 1108 1 -0.015510610944339476 1108 2 0.022807284432659003 1108 3 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-0.07223326436257338 2214 40-41 0.08250428296994272 2214 42-43 -0.005285080005712928 2214 44-45 -0.0603656340854144 2214 46-47 0.005778672562740894 2214 48-49 -0.004680739429119285 2214 50-51 -0.002858220404164058 2214 52-53 -0.027632232126137524 2214 54-55 0.07283063316707938 2214 56-57 -0.23290417960210874 2214 58-59 0.05634706202112483 2214 60-61 0.19964260991110905 2214 62-63 0.20892175964599602 2214 64-65 0.19584543308925362 2214 66-67 0.21329747145058775 2214 68-69 0.21964232096982172 2214 70-71 0.2504326162160062 2214 72-73 0.2678911225706244 2214 74-75 0.26201619287637 2214 76-77 0.2832407912221413 2214 78-79 0.3095655370514869 2214 80-81 0.29214712983818814 2214 82-83 0.24504072461370896 2214 84-85 0.26622719574076825 2214 86-87 0.31041610564181 2214 88-89 0.24576303430273683 2214 90-91 0.3492584345358516 2214 92-93 0.3432878239082413 2214 94-95 0.32127710634478035 2214 96-97 0.3058107817124913 2214 98-99 0.3414471916586237 2214 100-101 0.2006675803014204 2215 1 -0.18287900519175793 2215 2 -0.20306419347625848 2215 3 -0.19517257801294363 2215 4 -0.17077797833699293 2215 5 -0.2263673262006094 2215 6 -0.19381501238183318 2215 7 -0.21202102086376584 2215 8 -0.21976844988434863 2215 9 -0.3821263236294854 2215 10-11 -0.3483821866383252 2215 12-13 -0.35427942453789285 2215 14-15 -0.47137970664122975 2215 16-17 -0.4800054811550467 2215 18-19 -0.42299880478999796 2215 20-21 -0.4849339773545225 2215 22-23 -0.4003951582232901 2215 24-25 -0.3259994152898287 2215 26-27 -0.35730998201989905 2215 28-29 -0.2703715397406441 2215 30-31 -0.32889406671611 2215 32-33 -0.3696719569752318 2215 34-35 -0.31487625440882994 2215 36-37 -0.4106317833986708 2215 38-39 -0.3815997458425997 2215 40-41 -0.1067288198431271 2215 42-43 -0.23119421975088983 2215 44-45 -0.2427638364046274 2215 46-47 -0.25048204367785587 2215 48-49 -0.377912982300991 2215 50-51 -0.47111358234535317 2215 52-53 -0.30078634281609595 2215 54-55 -0.5364414141503318 2215 56-57 -0.2893271287456116 2215 58-59 -0.16624496128919475 2215 60-61 -0.29115552779106935 2215 62-63 -0.5993157524998836 2215 64-65 -0.4668067518732997 2215 66-67 -0.13660728617673357 2215 68-69 -0.10987599449084584 2215 70-71 -0.10672593690217269 2215 72-73 -0.0685690143211275 2215 74-75 -0.023178454648018487 2215 76-77 -0.019149445327457215 2215 78-79 0.0019162995699950613 2215 80-81 0.005916180159250217 2215 82-83 -0.033493306030205616 2215 84-85 -0.06814989331892107 2215 86-87 -0.08699232827603964 2215 88-89 -0.14980181420090943 2215 90-91 -0.03771679565771535 2215 92-93 -0.05888234513464852 2215 94-95 -0.09160800362462496 2215 96-97 -0.08419020802525878 2215 98-99 -0.08098268397304054 2215 100-101 -0.21057746689066192 2216 1 -0.1936633031010757 2216 2 -0.2611328580974117 2216 3 -0.3552838156303295 2216 4 -0.22080906838402825 2216 5 -0.24545083610743745 2216 6 -0.2689813980925919 2216 7 -0.34579304761513185 2216 8 -0.22655045211726588 2216 9 -0.3378826931664989 2216 10-11 -0.37174709730849287 2216 12-13 -0.37698650318285587 2216 14-15 -0.5398664283440198 2216 16-17 -0.5051918170186056 2216 18-19 -0.47620574074707633 2216 20-21 -0.45625751575767026 2216 22-23 -0.4766109941621295 2216 24-25 -0.4879970684903583 2216 26-27 -0.4096062698366012 2216 28-29 -0.39395450431013757 2216 30-31 -0.48771233811686443 2216 32-33 -0.718481317933886 2216 34-35 -0.6564737723321059 2216 36-37 -0.8016721353405103 2216 38-39 -0.7516134415221174 2216 40-41 -0.17952173036950114 2216 42-43 -0.2185571156268935 2216 44-45 -0.29954749056101804 2216 46-47 -0.46284979035257123 2216 48-49 -0.7738353758567129 2216 50-51 -0.9010674411943995 2216 52-53 -0.8610005926874038 2216 54-55 -0.9211896779548141 2216 56-57 -0.9701292512223461 2216 58-59 -0.7849056174589748 2216 60-61 -0.7830080275619764 2216 62-63 -0.7409434304575271 2216 64-65 -0.7561404863827903 2216 66-67 -0.7113719248338484 2216 68-69 -0.7564797190055508 2216 70-71 -0.6873200698740227 2216 72-73 -0.6989243126750821 2216 74-75 -0.6764253240596574 2216 76-77 -0.5629222499745552 2216 78-79 -0.5800902371851393 2216 80-81 -0.5560491454328407 2216 82-83 -0.6365443902813226 2216 84-85 -0.6682629018277453 2216 86-87 -0.6215165897833685 2216 88-89 -0.6502162683582462 2216 90-91 -0.6304402366323743 2216 92-93 -0.7123904007754085 2216 94-95 -0.8227204225238403 2216 96-97 -0.87363755140818 2216 98-99 -0.8782450342465324 2216 100-101 -0.9536763493727136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 29.0 11 391.0 12 1665.0 13 3350.0 14 5206.0 15 7658.0 16 10957.0 17 18399.0 18 44246.0 19 159983.0 20 175647.0 21 113203.0 22 92945.0 23 83945.0 24 81469.0 25 86903.0 26 102583.0 27 124199.0 28 148922.0 29 168000.0 30 182251.0 31 208076.0 32 251155.0 33 311662.0 34 400346.0 35 650067.0 36 906803.0 37 1221991.0 38 1271578.0 39 241530.0 40 88.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.549421101482395 38.00281460138423 16.895848300419757 14.551915996713612 2 16.161103633555125 22.821281009694786 49.23144025925879 11.786175097491295 3 15.516532497028019 35.60489124973305 19.580503691249223 29.298072561989706 4 32.000098371124004 20.916329846859057 32.170523516705494 14.913048265311444 5 17.22512302397358 35.53986171790186 17.556659152676932 29.67835610544763 6 17.51049822006214 19.697913019856408 30.553350292929704 32.238238467151746 7 32.639567212282486 20.244070631032386 17.395901514109685 29.720460642575446 8 45.853028169899936 19.921353982412203 17.697728432661076 16.527889415026785 9 17.12181920998659 32.61361758819162 18.135352730441777 32.12921047138001 10-11 38.29787143826922 19.480917061976776 17.790891257930642 24.43032024182336 12-13 31.19532081353485 19.67125670665632 18.121176546910657 31.012245932898175 14-15 30.742771241767247 19.926548147365033 18.372510528607695 30.95817008226003 16-17 30.46981964021892 27.58159538458516 18.35824954238347 23.590335432812452 18-19 23.08040977226661 20.267666980389517 26.306487957240222 30.34543529010365 20-21 22.191126330995935 34.3594647649757 19.816997201652462 23.6324117023759 22-23 15.228033735076668 27.211940445330036 34.43265655601847 23.127369263574828 24-25 15.237362031318483 20.113764226181786 42.31703147607426 22.33184226642547 26-27 15.174805911369596 20.076175432461934 49.314031015454304 15.434987640714168 28-29 15.34156334047419 19.52355868282761 49.87162992330869 15.263248053389514 30-31 15.270060536402475 19.44337066960348 49.98380268561649 15.302766108377558 32-33 15.252577047840168 19.432939938351268 50.01970955925638 15.294773454552187 34-35 15.31759951278026 19.49603314202317 49.87556618164709 15.310801163549485 36-37 15.405115892067089 19.427307626150718 49.69196835106958 15.47560813071261 38-39 15.802939459216054 19.748935973542693 48.21097411864208 16.23715044859918 40-41 18.471750880216618 21.823874134712188 40.772767367697554 18.93160761737364 42-43 18.447900122780165 22.3931687473243 41.386081644923486 17.772849484972046 44-45 19.187641081647044 23.857230708694694 39.091221832962155 17.863906376696107 46-47 18.858048347994067 25.14988522662177 37.99081502030954 18.001251405074623 48-49 18.93921865908003 26.608399678484723 35.81600048733281 18.636381175102436 50-51 19.20338611500065 26.68878556465944 34.86813251890711 19.2396958014328 52-53 19.26635918152398 26.541681159682483 34.79677105265724 19.395188606136294 54-55 19.29041487880211 25.931285508477654 34.94155751735593 19.836742095364304 56-57 19.25886827696616 25.738733926886226 35.29583490159425 19.706562894553365 58-59 19.471588765734964 25.836052084118055 35.00058019175868 19.69177895838831 60-61 19.78386054932075 25.91251584573655 34.50223009882199 19.801393506120704 62-63 20.044006944209862 26.188202334137596 33.945274278057006 19.82251644359554 64-65 20.085680400981055 26.345511329851806 33.39061519689701 20.178193072270126 66-67 20.203315870103193 26.326621529962136 32.99561131929387 20.474451280640803 68-69 20.366886131325167 26.580019043858115 32.59057952323078 20.46251530158594 70-71 20.587556872572787 26.763588606871252 32.311691733164935 20.337162787391026 72-73 20.667391541242306 26.820484147055218 32.228931371583215 20.283192940119264 74-75 20.754162288069413 27.004007697379627 31.92231667664292 20.31951333790804 76-77 20.89516333920017 27.030735705721952 31.712020115680712 20.362080839397166 78-79 20.85686117226343 26.8966716255994 31.792643582304734 20.453823619832434 80-81 20.78501095367737 26.951226700201687 31.772251145722567 20.49151120039837 82-83 20.788979384932354 26.961733127419873 31.787891703352038 20.461395784295732 84-85 20.783710847371843 26.64195268226257 32.03334628229841 20.54099018806717 86-87 20.700949218703897 26.673563590911243 32.19774125848774 20.427745931897125 88-89 20.715421125016015 26.779777394480675 31.916571798396404 20.588229682106903 90-91 20.92860043314438 26.586699050025025 31.75851020721703 20.726190309613568 92-93 20.98072780654749 26.60893533659009 31.684024250465935 20.726312606396487 94-95 21.000072506302605 26.742069923495247 31.593603728604812 20.664253841597333 96-97 20.8540210680984 26.83237065787244 31.44403297863665 20.869575295392515 98-99 21.05559000272358 26.732918299530745 31.421736937240496 20.78975476050518 100-101 21.1416506024454 26.68984559832328 31.11274419112153 21.055759608109796 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 96495.0 1 52581.0 2 6316.5 3 3287.0 4 2362.5 5 2051.0 6 1907.5 7 1766.5 8 1705.5 9 1671.5 10 1757.0 11 1893.0 12 2666.5 13 4598.0 14 5698.0 15 11023.5 16 215689.0 17 219579.0 18 17159.5 19 9256.0 20 8878.0 21 10271.0 22 12792.5 23 16649.5 24 21299.0 25 27137.5 26 35039.5 27 45218.5 28 58343.5 29 73638.5 30 91292.5 31 111438.5 32 133281.0 33 154410.5 34 171706.0 35 188105.0 36 201749.0 37 212933.5 38 222418.5 39 229748.0 40 237296.0 41 244638.5 42 253538.5 43 261810.0 44 264137.0 45 260872.5 46 256220.0 47 248165.0 48 239923.0 49 233695.5 50 218138.0 51 201416.0 52 183900.0 53 166048.5 54 151211.5 55 135339.5 56 123049.0 57 110368.5 58 98868.5 59 89852.0 60 79712.5 61 69119.5 62 60470.5 63 52605.0 64 45056.0 65 37396.0 66 30330.0 67 24154.0 68 18500.5 69 13663.5 70 9715.0 71 6636.0 72 4460.5 73 3006.0 74 1960.5 75 1123.5 76 588.5 77 339.5 78 183.0 79 77.0 80 19.5 81 7.5 82 5.0 83 2.5 84 4.5 85 5.0 86 2.0 87 1.5 88 1.5 89 2.0 90 2.5 91 1.5 92 1.0 93 0.5 94 2.0 95 2.5 96 1.0 97 3.0 98 4.0 99 4.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0015829836046713282 76-77 0.00239567608028384 78-79 0.0066711451911148825 80-81 0.006416737111792705 82-83 0.0015052478026562183 84-85 5.017492675520727E-4 86-87 0.001590050495763611 88-89 0.0012084383767803444 90-91 1.1307025747652345E-4 92-93 2.1200673276848146E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7075247.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.37211331638641 #Duplication Level Percentage of deduplicated Percentage of total 1 80.95601582813863 37.541015396256164 2 9.692698615127904 8.989418370445854 3 3.019445577474691 4.200542174139545 4 1.5278874360957937 2.8340547728526895 5 0.8799148453088608 2.0401755457716555 6 0.6217583917539125 1.7299350358695964 7 0.4643601387376181 1.507335268120762 8 0.34900981427617667 1.294745812491667 9 0.28899955552244644 1.2061368123364975 >10 2.049909245698466 17.685742945272985 >50 0.10556504173785047 3.2737829652207195 >100 0.040650268513627436 3.259162163515577 >500 0.0014042197473379244 0.43364710377059135 >1k 0.0016789256752871905 1.7511079639584295 >5k 2.747332922260599E-4 0.7715321810144239 >10k+ 4.2736289901831545E-4 11.481665488962799 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 511802 7.233697989624956 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111184 1.571450438408723 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 23566 0.3330767109614689 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 20017 0.2829159179884462 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 19473 0.2752271404800426 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC 17841 0.2521608079548318 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT 17326 0.2448819101297806 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15698 0.2218721127333081 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 15041 0.21258621783804862 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13504 0.19086259462037156 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 12312 0.17401512625636958 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 11733 0.16583166637150618 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 11400 0.16112511690404588 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA 10212 0.14433418366878215 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG 8750 0.1236705941149475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.5440807932217775E-4 0.0 0.0 0.0 0.0 2 3.109432080604394E-4 0.0 0.0 0.0 0.0 3 3.6747833679870115E-4 0.0 0.0 0.0 0.0 4 4.381472477215283E-4 0.0 0.0 0.0 0.0 5 5.370837230134863E-4 0.0 0.0 0.0 0.0 6 7.208228914128369E-4 0.0 0.0 0.0 0.0 7 8.197593667047949E-4 0.0 0.0 0.0 1.4133782184565429E-5 8 9.752309707350146E-4 0.0 0.0 0.0 1.4133782184565429E-5 9 0.001286174178795454 0.0 0.0 0.0 1.4133782184565429E-5 10-11 0.001512314693748501 0.0 0.0 0.0 1.4133782184565429E-5 12-13 0.0017737896641629614 0.0 0.0 0.0 1.4133782184565429E-5 14-15 0.002077665981131118 0.0 0.0 0.0 1.4133782184565429E-5 16-17 0.002487545664483516 0.0 0.0 0.0 1.4133782184565429E-5 18-19 0.003031696278589285 0.0 0.0 0.0 1.4133782184565429E-5 20-21 0.0035687800016027706 0.0 0.0 0.0 1.4133782184565429E-5 22-23 0.004056395486970278 1.4133782184565429E-5 0.0 0.0 2.1200673276848143E-5 24-25 0.0046217467743528955 1.4133782184565429E-5 0.0 0.0 2.8267564369130858E-5 26-27 0.005194164952827795 1.4133782184565429E-5 0.0 0.0 2.8267564369130858E-5 28-29 0.0057241817847489985 1.4133782184565429E-5 0.0 0.0 2.8267564369130858E-5 30-31 0.0063743357652390085 1.4133782184565429E-5 0.0 0.0 2.8267564369130858E-5 32-33 0.006996222181359887 1.4133782184565429E-5 0.0 0.0 2.8267564369130858E-5 34-35 0.007455570102358264 1.4133782184565429E-5 0.0 0.0 4.2401346553696286E-5 36-37 0.007851316003526097 1.4133782184565429E-5 0.0 0.0 4.2401346553696286E-5 38-39 0.008225861231417081 2.1200673276848143E-5 0.0 0.0 4.2401346553696286E-5 40-41 0.008805346300984262 4.2401346553696286E-5 0.0 0.0 4.2401346553696286E-5 42-43 0.009356563806182314 4.2401346553696286E-5 0.0 0.0 4.2401346553696286E-5 44-45 0.009745242816257863 4.2401346553696286E-5 0.0 0.0 4.2401346553696286E-5 46-47 0.010056186024318303 4.2401346553696286E-5 0.0 7.0668910922827145E-6 4.2401346553696286E-5 48-49 0.010352995450194177 4.2401346553696286E-5 0.0 1.4133782184565429E-5 4.2401346553696286E-5 50-51 0.010663938658254615 4.2401346553696286E-5 0.0 1.4133782184565429E-5 4.2401346553696286E-5 52-53 0.012098517549988006 4.2401346553696286E-5 0.0 1.4133782184565429E-5 4.2401346553696286E-5 54-55 0.013815772085412709 4.9468237645979E-5 0.0 1.4133782184565429E-5 4.2401346553696286E-5 56-57 0.015787434700159586 5.6535128738261716E-5 0.0 1.4133782184565429E-5 4.2401346553696286E-5 58-59 0.017822699334737006 5.6535128738261716E-5 0.0 1.4133782184565429E-5 4.2401346553696286E-5 60-61 0.019921565989144973 5.6535128738261716E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 62-63 0.022741255534965775 5.6535128738261716E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 64-65 0.025758818031370492 7.066891092282714E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 66-67 0.029384133161711526 7.066891092282714E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 68-69 0.03396347858951072 7.066891092282714E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 70-71 0.039638192136613744 7.066891092282714E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 72-73 0.046775752139819285 7.066891092282714E-5 0.0 1.4133782184565429E-5 5.6535128738261716E-5 74-75 0.05568003491609551 7.066891092282714E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 76-77 0.06731213765399285 7.066891092282714E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 78-79 0.08309957235415244 7.066891092282714E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 80-81 0.10191870333290132 7.066891092282714E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 82-83 0.12446208591728317 8.480269310739257E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 84-85 0.15261658002893752 8.480269310739257E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 86-87 0.18737861731187616 9.186958419967529E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 88-89 0.22786483637956384 9.8936475291958E-5 0.0 1.4133782184565429E-5 7.066891092282714E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 106815 0.0 94.077995 1 TCGACGG 109195 0.0 92.03183 2 ATATCTC 100060 0.0 91.110634 1 TATCTCG 100725 0.0 90.33463 2 CGACGGC 112405 0.0 89.30644 3 GACGGCG 113160 0.0 88.718994 4 ACGGCGC 113610 0.0 88.33832 5 CGGCGCG 115485 0.0 86.875275 6 ATCTCGA 105160 0.0 86.46164 3 TCTCGAG 104860 0.0 85.19152 4 CTCGAGG 107755 0.0 82.898315 5 TCGAGGG 109075 0.0 81.94301 6 AGGGCGC 112400 0.0 79.89086 9 CGAGGGC 112025 0.0 79.85301 7 GAGGGCG 113395 0.0 79.08932 8 CTCGACG 2130 0.0 71.137375 5 TCTCGAC 2415 0.0 63.135647 4 AATTCGA 1885 0.0 47.62524 1 GGCGCGC 228910 0.0 44.81625 7 CGCGCCG 229005 0.0 44.785213 9 >>END_MODULE