FastQCFastQC Report
Thu 26 May 2016
SRR1051295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7075247
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4976667.033902844663939No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT291990.412692306007126No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT207670.2935162546268703No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148580.20999973569827313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG62500.088.9264451
TCGACGG66900.083.212062
TATCTCG91700.060.0860182
CGACGGC92550.060.0474053
GACGGCG94650.058.865684
ACGGCGC97950.056.9794465
ATATCTC99950.055.5592231
CGGCGCG123550.045.249996
ATCTCGA152850.040.4400631
AATTCGA23800.037.5879821
TCTCGAG166550.037.103722
CTCGAGG195200.031.8039113
TCGAGGG200350.031.3183044
CGAGGCG16250.030.3993825
CGAGGGC220550.028.5144945
AGGGCGC221000.028.3274777
GGGCGCG226150.027.682398
CGGGTCG9150.026.73442646-47
GAGGGCG235550.026.658346
AACGACG6350.026.18056944-45
Produced by FastQC (version 0.11.5)