FastQCFastQC Report
Thu 26 May 2016
SRR1051294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609469
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1263292.740641058655563No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA139410.30244264577980673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131030.28426267754485385No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA113740.24675293401474227No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT107470.23315049954777872No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC94210.2043836285697984No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC91180.19781020330107438No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT77270.16763319158887932No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC75450.1636847975330781No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG71440.15498531392661496No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA60590.13144681090164614No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.1103163943612594No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG46540.10096607656977408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG902600.094.151621
TCGACGG925550.091.827292
ATATCTC409350.090.102161
CGACGGC948450.089.5350343
TATCTCG414250.088.829972
GACGGCG957750.088.655714
ACGGCGC958950.088.5150455
CGGCGCG968650.087.623766
ATCTCGA439350.083.679453
TCTCGAG429850.082.357414
CTCGAGG446750.079.422685
TCGAGGG454350.078.08376
AGGGCGC467600.076.257129
CGAGGGC473300.075.188217
GAGGGCG480100.074.23218
CTCGACG20300.064.814515
CGCGCCG1438100.059.994439
GGCGCGC1439450.059.9480637
GCGCGCC1440000.059.9218648
TCTCGAC22300.059.2145464