##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051294_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4609469
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06662155662615	33.0	31.0	34.0	30.0	34.0
2	32.235531901830775	33.0	31.0	34.0	31.0	34.0
3	32.27536208617522	34.0	31.0	34.0	30.0	34.0
4	36.02049541932053	37.0	35.0	37.0	35.0	37.0
5	35.84953342781999	37.0	35.0	37.0	35.0	37.0
6	35.9060379839847	37.0	36.0	37.0	35.0	37.0
7	35.88016103373295	37.0	35.0	37.0	35.0	37.0
8	35.95273902482043	37.0	36.0	37.0	35.0	37.0
9	37.55981589202574	39.0	38.0	39.0	35.0	39.0
10-11	37.55031306209023	39.0	38.0	39.0	35.0	39.0
12-13	37.58324570574181	39.0	38.0	39.0	35.0	39.0
14-15	38.94446724774589	41.0	39.0	41.0	35.5	41.0
16-17	38.85871561344702	40.5	38.5	41.0	35.0	41.0
18-19	38.79862572022938	40.5	38.5	41.0	35.0	41.0
20-21	38.906633171846906	40.5	39.0	41.0	35.0	41.0
22-23	38.865265174795624	40.5	39.0	41.0	34.5	41.0
24-25	38.834491673552854	40.5	38.5	41.0	34.5	41.0
26-27	38.889374784817946	41.0	39.0	41.0	35.0	41.0
28-29	38.53584415037828	39.5	38.0	41.0	34.5	41.0
30-31	37.71581173449697	39.0	36.0	40.5	34.0	41.0
32-33	37.38167660960514	38.0	35.0	41.0	34.0	41.0
34-35	37.10301728897623	38.0	35.0	40.5	34.0	41.0
36-37	36.98766322107818	38.0	35.0	40.0	33.0	41.0
38-39	36.61495662515574	38.0	35.0	40.5	32.0	41.0
40-41	34.897441006762385	38.0	33.5	40.5	23.0	41.0
42-43	34.83638169602616	38.0	33.5	40.0	23.0	41.0
44-45	34.78684703162121	38.0	33.0	40.0	23.0	41.0
46-47	35.026522143873834	38.0	33.0	40.0	23.0	41.0
48-49	34.695795329136615	38.0	33.0	40.5	23.0	41.0
50-51	34.7133551608656	38.0	33.0	40.5	24.0	41.0
52-53	34.53882052357875	37.5	33.0	40.0	24.0	41.0
54-55	34.14820438102523	37.0	32.0	40.0	23.0	41.0
56-57	33.790947178514486	37.0	32.0	40.0	22.0	41.0
58-59	33.425340966605916	36.0	32.0	40.0	19.0	41.0
60-61	33.106647859005015	36.0	32.0	40.0	18.0	41.0
62-63	32.746861189434185	35.0	31.5	40.0	18.0	41.0
64-65	32.36610279839174	35.0	31.0	39.0	18.0	41.0
66-67	31.957373723524334	35.0	31.0	39.0	15.5	41.0
68-69	31.600223583237028	35.0	31.0	38.5	11.0	40.5
70-71	31.20906659747576	35.0	30.5	37.5	8.5	40.0
72-73	30.840833076434617	35.0	30.0	37.0	7.0	39.5
74-75	30.49330833985433	35.0	30.0	36.5	7.0	39.0
76-77	30.168256148376308	35.0	30.0	36.0	6.0	39.0
78-79	29.91990650116098	35.0	30.0	36.0	2.0	37.5
80-81	29.670382857548233	34.0	30.0	35.0	2.0	37.0
82-83	29.48867385809515	34.0	30.0	35.0	2.0	37.0
84-85	29.29761584251895	34.0	30.0	35.0	2.0	36.0
86-87	29.10216795036478	34.0	29.5	35.0	2.0	36.0
88-89	28.901465331473105	34.0	29.0	35.0	2.0	36.0
90-91	28.695430102686448	34.0	29.0	35.0	2.0	35.0
92-93	28.44450662321408	34.0	29.0	35.0	2.0	35.0
94-95	28.19923628947282	34.0	29.0	35.0	2.0	35.0
96-97	27.904584237360098	34.0	29.0	35.0	2.0	35.0
98-99	27.541512916129818	34.0	29.0	35.0	2.0	35.0
100-101	25.843594457409303	32.5	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27142232580969505
1101	2	0.29030789791509193
1101	3	0.25301802720864686
1101	4	0.2335811550199196
1101	5	0.2818261953974712
1101	6	0.2674535249046883
1101	7	0.2737302556478056
1101	8	0.2147432511202112
1101	9	0.3046806584854096
1101	10-11	0.28081539276666945
1101	12-13	0.273068996219898
1101	14-15	0.32725011780612334
1101	16-17	0.3832341043013372
1101	18-19	0.3599698719775475
1101	20-21	0.3408599258728131
1101	22-23	0.3664777613378547
1101	24-25	0.37301693276207715
1101	26-27	0.34802074020377205
1101	28-29	0.3108138791166155
1101	30-31	0.33132545769214516
1101	32-33	0.3191800280589945
1101	34-35	0.35888368018863304
1101	36-37	0.3261937889757007
1101	38-39	0.30789072745803736
1101	40-41	-0.003264754491119959
1101	42-43	0.0682960447095482
1101	44-45	0.08952070543584512
1101	46-47	0.204344609008956
1101	48-49	0.13380101637643094
1101	50-51	0.15841732559736954
1101	52-53	0.05081899150520286
1101	54-55	-0.03170515348078595
1101	56-57	-0.096143391333257
1101	58-59	-0.10426884405679004
1101	60-61	-0.12668734919898839
1101	62-63	-0.11447726113242851
1101	64-65	-0.27855538762641885
1101	66-67	-0.21107841975586794
1101	68-69	-0.2729531932392071
1101	70-71	-0.3482020030523181
1101	72-73	-0.3174279043873476
1101	74-75	-0.39202550583575047
1101	76-77	-0.3721482643600531
1101	78-79	-0.3702943225328461
1101	80-81	-0.36669868146658047
1101	82-83	-0.4153868889922734
1101	84-85	-0.4573432375640962
1101	86-87	-0.4073707875088175
1101	88-89	-0.35902171225182755
1101	90-91	-0.3726413197920806
1101	92-93	-0.38650119400395155
1101	94-95	-0.28818277948946047
1101	96-97	-0.2489807594216913
1101	98-99	-0.2542247332730341
1101	100-101	-0.24515270834852743
1102	1	0.20419004167531796
1102	2	0.22965158390338303
1102	3	0.2192860638971439
1102	4	0.12668543015276157
1102	5	0.16656247654408673
1102	6	0.20968360888220872
1102	7	0.17392243350468561
1102	8	0.14160056119669662
1102	9	0.2221542138468351
1102	10-11	0.19463013143406727
1102	12-13	0.18658426418632956
1102	14-15	0.22763660561992793
1102	16-17	0.28355156901243106
1102	18-19	0.31922446319837405
1102	20-21	0.3112670940181417
1102	22-23	0.3177250388320729
1102	24-25	0.30347535152228033
1102	26-27	0.31045151630752343
1102	28-29	0.2935156796335576
1102	30-31	0.23979078640991247
1102	32-33	0.26410140001176785
1102	34-35	0.30438565457355793
1102	36-37	0.250293842737463
1102	38-39	0.18673067135096488
1102	40-41	-0.11525501399408711
1102	42-43	-0.07422824621703938
1102	44-45	-0.14475619438843523
1102	46-47	-0.023859005881490702
1102	48-49	-0.11652176507734424
1102	50-51	-0.12150508300072715
1102	52-53	-0.13001128233727854
1102	54-55	0.03437174438594326
1102	56-57	-0.1852806553322779
1102	58-59	-0.18562384933520093
1102	60-61	-0.17977789784671927
1102	62-63	-0.09893840785855446
1102	64-65	-0.24716944092131854
1102	66-67	-0.14943266377480313
1102	68-69	-0.1717553187476284
1102	70-71	-0.15316975046720316
1102	72-73	-0.20176533112420358
1102	74-75	-0.24683278570644163
1102	76-77	-0.20033566199041886
1102	78-79	-0.20726753095005535
1102	80-81	-0.14089363757867446
1102	82-83	-0.1966194689191667
1102	84-85	-0.3223671419176739
1102	86-87	-0.2954296743668081
1102	88-89	-0.3090613815734997
1102	90-91	-0.2510983688546311
1102	92-93	-0.24301276161306262
1102	94-95	-0.20245011146278102
1102	96-97	-0.23200361139893744
1102	98-99	-0.3387168245429599
1102	100-101	-0.14298578814690188
1103	1	0.1523418066298916
1103	2	0.1906593631328377
1103	3	0.20488409192553547
1103	4	0.14828662760087497
1103	5	0.1705531109700118
1103	6	0.21365867118432647
1103	7	0.24383437408766184
1103	8	0.18813136656723373
1103	9	0.30741590915104666
1103	10-11	0.2355759621142397
1103	12-13	0.2724612889239566
1103	14-15	0.2825883565574969
1103	16-17	0.34952599434551956
1103	18-19	0.3995562609344674
1103	20-21	0.29576561064236273
1103	22-23	0.311267438371857
1103	24-25	0.31173058915267404
1103	26-27	0.3088713020342553
1103	28-29	0.25107211663605966
1103	30-31	0.2746728991451306
1103	32-33	0.2739939112789216
1103	34-35	0.2778916979198911
1103	36-37	0.3148010093306368
1103	38-39	0.21234933003319867
1103	40-41	-0.003534980911609864
1103	42-43	-0.027590772846316725
1103	44-45	0.007594401981997123
1103	46-47	0.03430046633123851
1103	48-49	0.03231334574859801
1103	50-51	0.050347324451706754
1103	52-53	-7.969741585398538E-4
1103	54-55	-0.03424871592603296
1103	56-57	-0.029682768144617455
1103	58-59	-0.06472014051639263
1103	60-61	-0.015361284306216305
1103	62-63	-0.08597823011753292
1103	64-65	-0.11777799197480476
1103	66-67	-0.1987403581686671
1103	68-69	-0.29333715897696777
1103	70-71	-0.36441232714356886
1103	72-73	-0.34681778864216994
1103	74-75	-0.31503549183556956
1103	76-77	-0.19929657377997145
1103	78-79	-0.2977489371845117
1103	80-81	-0.32603212657462777
1103	82-83	-0.34160050006035547
1103	84-85	-0.43112676336290434
1103	86-87	-0.3669623209366435
1103	88-89	-0.42040740074553185
1103	90-91	-0.3462585171448431
1103	92-93	-0.4000610674309648
1103	94-95	-0.3168350735613217
1103	96-97	-0.34024117896529305
1103	98-99	-0.2845867074812496
1103	100-101	-0.25215790320557474
1104	1	0.060899838584553834
1104	2	0.10555858728795897
1104	3	0.12800127518926985
1104	4	0.13576924613245467
1104	5	0.150947015760849
1104	6	0.17280483360672605
1104	7	0.16523987719740774
1104	8	0.19391283965072148
1104	9	0.26251319214746616
1104	10-11	0.1937252126103175
1104	12-13	0.2098882331107177
1104	14-15	0.2672163656816551
1104	16-17	0.169766845819332
1104	18-19	0.25842100052447137
1104	20-21	0.2619406171867382
1104	22-23	0.31979092742152204
1104	24-25	0.2858900695791107
1104	26-27	0.26308005450731287
1104	28-29	0.26079612041775846
1104	30-31	0.2991316606080545
1104	32-33	0.31257343678296223
1104	34-35	0.29933593920858215
1104	36-37	0.35715108444741617
1104	38-39	0.2994327185840504
1104	40-41	0.049272726087444596
1104	42-43	-0.008208292863564282
1104	44-45	0.0679276173782597
1104	46-47	0.08975624617399092
1104	48-49	0.17618022823926793
1104	50-51	0.25734689805094035
1104	52-53	0.07837096764509255
1104	54-55	0.15215254455052474
1104	56-57	0.1666820793657635
1104	58-59	0.28636764665975534
1104	60-61	0.24000690609567954
1104	62-63	0.20748648444332218
1104	64-65	0.24652327362325366
1104	66-67	0.20337027954566977
1104	68-69	0.12248603130716518
1104	70-71	0.10390296064425897
1104	72-73	0.13588223854119263
1104	74-75	0.06434600240450195
1104	76-77	0.04511352753442566
1104	78-79	0.05195053283629392
1104	80-81	0.014924786495019049
1104	82-83	-0.02529995801084084
1104	84-85	0.00540270233857143
1104	86-87	0.031425000668864556
1104	88-89	-0.07475982773036804
1104	90-91	-0.02515905947934982
1104	92-93	0.046566058045279135
1104	94-95	0.031504997775936516
1104	96-97	0.051506398651675056
1104	98-99	-0.0143752103518473
1104	100-101	0.017001653079454115
1105	1	0.04077546569299528
1105	2	0.0937807010766818
1105	3	0.051598057292984834
1105	4	0.09073707064077041
1105	5	0.09492255970938857
1105	6	0.13305953496747946
1105	7	0.18332665903830048
1105	8	0.169446622553302
1105	9	0.27062041436390416
1105	10-11	0.22664013066285804
1105	12-13	0.23355849295936792
1105	14-15	0.2821262609160513
1105	16-17	0.27606808002725103
1105	18-19	0.31466042267971517
1105	20-21	0.30411947096862946
1105	22-23	0.3180185728062952
1105	24-25	0.2743525015870887
1105	26-27	0.2620601432409515
1105	28-29	0.27401039922506953
1105	30-31	0.3060562593256009
1105	32-33	0.3301440644294331
1105	34-35	0.275114565280127
1105	36-37	0.24962681212132054
1105	38-39	0.3101795361187314
1105	40-41	0.19921462077438434
1105	42-43	0.19840710922223082
1105	44-45	0.11215949662830127
1105	46-47	0.10639182085748189
1105	48-49	0.16329224169560064
1105	50-51	0.16550783699907612
1105	52-53	0.14378574808957723
1105	54-55	0.12453733437218517
1105	56-57	0.16969042709215643
1105	58-59	0.2079205151803336
1105	60-61	0.12648719938184172
1105	62-63	-0.038197271408741074
1105	64-65	0.039555793259054894
1105	66-67	0.005022766383639521
1105	68-69	-0.056493581376976465
1105	70-71	-0.024754624386361
1105	72-73	0.01226055061841791
1105	74-75	0.004604269177683307
1105	76-77	0.01741476075057946
1105	78-79	-0.02861191965173049
1105	80-81	0.04030944441130302
1105	82-83	-0.01274557803309051
1105	84-85	0.0481918664601082
1105	86-87	0.03887145625716215
1105	88-89	0.1302544859872441
1105	90-91	0.08070598197414469
1105	92-93	0.10535711793478342
1105	94-95	0.13332950708543123
1105	96-97	0.047793088555508234
1105	98-99	0.058556999207130644
1105	100-101	0.04800282733489425
1106	1	0.05626624820174442
1106	2	0.014912203822369463
1106	3	0.07331359496136969
1106	4	0.07590977665468301
1106	5	0.07669385565245079
1106	6	0.06788501370333222
1106	7	0.09554741870596217
1106	8	0.09481896726446593
1106	9	0.16835047791707325
1106	10-11	0.1275963889564622
1106	12-13	0.17048995318742755
1106	14-15	0.17447160790549532
1106	16-17	0.1446056193050964
1106	18-19	0.17181478981261478
1106	20-21	0.10783959335726223
1106	22-23	0.1630935174023378
1106	24-25	0.15981348778245064
1106	26-27	0.14787629236716526
1106	28-29	0.1060594672833659
1106	30-31	0.12661100756398014
1106	32-33	0.12796134735899756
1106	34-35	0.14599239408553188
1106	36-37	0.17460031140929289
1106	38-39	0.19339269406200543
1106	40-41	0.017687655571862138
1106	42-43	0.03543950027566467
1106	44-45	0.042331819207007015
1106	46-47	0.09237435174343034
1106	48-49	0.1054322324349144
1106	50-51	0.14831647079515164
1106	52-53	0.019726863119167604
1106	54-55	-0.012608461284990824
1106	56-57	0.025826870813084213
1106	58-59	-0.04197464683802821
1106	60-61	-0.0799954523590003
1106	62-63	-0.08585717602559129
1106	64-65	-0.15594192505876237
1106	66-67	-0.07164473995760545
1106	68-69	-0.059798525943893566
1106	70-71	-0.08330821455333393
1106	72-73	-0.043979687557939684
1106	74-75	-0.1076897072007661
1106	76-77	-0.06555333841061639
1106	78-79	-0.003482109845304393
1106	80-81	-0.042035329852140535
1106	82-83	-0.025220933826989977
1106	84-85	0.005912310157746958
1106	86-87	-0.006977930392107368
1106	88-89	-0.06887737301343577
1106	90-91	-0.0016261729026147975
1106	92-93	0.06220051425827222
1106	94-95	0.09037930318526932
1106	96-97	0.03810952938368928
1106	98-99	-0.12874394210204443
1106	100-101	-0.04688209841943092
1107	1	0.06350840331358398
1107	2	0.012883192081865502
1107	3	0.022867713076507812
1107	4	0.04025198024104526
1107	5	0.07600823890696518
1107	6	0.07687388636033177
1107	7	0.09250808674141098
1107	8	0.055246841393717716
1107	9	0.13963252239517487
1107	10-11	0.12795459734825698
1107	12-13	0.10445998253907618
1107	14-15	0.06783786805905123
1107	16-17	0.0995554697353711
1107	18-19	0.08053477023958777
1107	20-21	0.10698281922057618
1107	22-23	0.12586940084614895
1107	24-25	0.12149235679241599
1107	26-27	0.15447504258059297
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2216	22-23	-0.6132997787999344
2216	24-25	-0.6987341651861385
2216	26-27	-0.570598314152619
2216	28-29	-0.6221899728224614
2216	30-31	-0.6276089458459353
2216	32-33	-0.803111336050236
2216	34-35	-0.726651665041075
2216	36-37	-0.8503835349949895
2216	38-39	-0.8608351626750235
2216	40-41	-0.0870058243983749
2216	42-43	-0.12026058879114743
2216	44-45	-0.13103015900340154
2216	46-47	-0.32529369214216786
2216	48-49	-0.5738598084775361
2216	50-51	-0.625719392685312
2216	52-53	-0.6284744673042937
2216	54-55	-0.6940384944276232
2216	56-57	-0.742528311507435
2216	58-59	-0.530112231620123
2216	60-61	-0.5066160438081937
2216	62-63	-0.48890472394030127
2216	64-65	-0.46164702396067625
2216	66-67	-0.38841574580759186
2216	68-69	-0.3947327166945449
2216	70-71	-0.4503068070811054
2216	72-73	-0.3700397395214523
2216	74-75	-0.40193494709415134
2216	76-77	-0.2907385565937908
2216	78-79	-0.32862231583973056
2216	80-81	-0.24237086390472484
2216	82-83	-0.3028114210244297
2216	84-85	-0.3854374462754535
2216	86-87	-0.3659525642508754
2216	88-89	-0.3547569168714233
2216	90-91	-0.34659269721224817
2216	92-93	-0.3054852999703819
2216	94-95	-0.3942363744622348
2216	96-97	-0.42060498856331563
2216	98-99	-0.49886143453108645
2216	100-101	-0.5589159374269208
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	39.0
11	376.0
12	1346.0
13	2547.0
14	3824.0
15	5095.0
16	6784.0
17	11547.0
18	29640.0
19	103492.0
20	117476.0
21	76481.0
22	63807.0
23	57757.0
24	56750.0
25	61071.0
26	71798.0
27	87968.0
28	106070.0
29	121399.0
30	133322.0
31	152776.0
32	183498.0
33	221477.0
34	272071.0
35	333446.0
36	474586.0
37	828833.0
38	869730.0
39	154407.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3942436753561	31.587434474556613	19.07276087549347	14.94556097459382
2	16.785187187504675	21.96062062680105	48.856994157027636	12.397198028666642
3	16.433975366793877	30.024477873698686	19.66875143319111	33.87279532631633
4	36.86511396431997	20.171108646136897	27.3224095877421	15.641367801801032
5	17.989273818741378	38.87430417690194	18.335929800157025	24.80049220419966
6	18.086898946494706	19.127344169143996	26.227814960898964	36.55794192346234
7	37.28522743075178	19.495130567100027	18.22153484490296	24.99810715724523
8	45.49040247369057	19.241890985707897	18.497011260950014	16.77069527965152
9	17.50618129767225	26.978530498849217	18.829609223969182	36.68567897950935
10-11	35.514079821341674	18.91390309816597	18.592629649966188	26.979387430526163
12-13	31.087463653622578	19.110986536627102	18.866446438841436	30.935103370908884
14-15	30.729884505134976	19.38276404505595	19.123493400215946	30.76385804959313
16-17	30.42055386423035	28.952619054385657	19.267653172198358	21.359173909185635
18-19	20.800356830689175	19.72709871787835	29.091951806162484	30.38059264526999
20-21	20.045421717772697	33.454981474004924	20.33184299536454	26.167753812857836
22-23	15.814316139234258	28.764462891495747	34.58354964530621	20.83767132396378
24-25	15.788662425107969	19.64819592018083	44.35899232644802	20.204149328263192
26-27	15.781503249072722	19.58614972787538	48.784458687106905	15.847888335944987
28-29	15.835999764831914	19.00858862485028	49.38218480263128	15.773226807686525
30-31	15.799281869560247	18.888184300621177	49.47134908597931	15.841184743839257
32-33	15.801169288696812	18.861261459834093	49.488715511482994	15.848853739986101
34-35	15.87467016265865	18.996211928098443	49.330400095976344	15.79871781326656
36-37	15.923645435081568	18.975938443235002	49.171965360869116	15.928450760814314
38-39	16.573210493442954	19.3437031467182	47.24549617320346	16.83759018663538
40-41	19.943566167816726	22.001590638748194	37.66684405513954	20.38799913829554
42-43	20.040930961896045	22.79298331326233	38.25372293424687	18.912362790594752
44-45	20.98696183877145	24.569728096663628	35.442900255973086	19.000409808591836
46-47	20.318945631264686	26.27750615092541	34.343088108413355	19.060460109396548
48-49	20.27866984244823	27.661711142866995	32.338692374327714	19.720926640357057
50-51	20.488129977661202	27.843955561909628	31.415223749199743	20.25269071122943
52-53	20.548570778976927	27.574314959054934	31.42387984386054	20.453234418107595
54-55	20.65455912600779	26.767063624899095	31.737527684859145	20.84084956423397
56-57	20.60803532901512	26.557364850484948	32.1093384075259	20.72526141297403
58-59	20.70692958342924	26.589364197915206	32.08805612967567	20.61565008897988
60-61	21.067209693784687	26.708792270866777	31.57084905007497	20.653148985273575
62-63	21.384784234366258	26.846443700998968	30.964531923308304	20.804240141326474
64-65	21.52920434002268	27.04551218372442	30.264158409569518	21.161125066683386
66-67	21.650639151711403	27.16961541557173	29.740312821281584	21.439432611435286
68-69	21.678408076939014	27.442434258696608	29.402681740564912	21.476475923799466
70-71	21.916906264040392	27.759770160077007	29.006161013340147	21.317162562542453
72-73	21.986762466566105	27.909407786450025	28.717494357809976	21.386335389173894
74-75	22.165168309945173	28.02457508955103	28.47678311826116	21.333473482242635
76-77	22.25800576458657	28.22923750320137	28.108949406212435	21.40380732599962
78-79	22.2097916329249	28.097157620945033	28.137544681507944	21.55550606462212
80-81	22.157609487433803	28.008358553887298	28.244800230323442	21.58923172835546
82-83	22.191559119519027	28.0233543862694	28.21494062421187	21.570145869999703
84-85	22.193059909533673	27.81649654514096	28.440453850242438	21.549989695082928
86-87	22.149007077604594	27.78331055559635	28.52469796221785	21.542984404581205
88-89	22.247866594784803	27.794210328208678	28.295109032274162	21.662814044732357
90-91	22.32426231908818	27.735554850484196	28.07884867270316	21.861334157724464
92-93	22.420972670258625	27.782201645722264	27.99187888487288	21.804946799146236
94-95	22.45976705776739	27.78472965107261	27.93390084627969	21.821602444880313
96-97	22.382491345532422	27.813290424558662	27.747523630162174	22.05669459974674
98-99	22.416909626683683	27.77055231307554	27.772342107084352	22.040195953156427
100-101	22.616477082284312	27.587038767372118	27.48003078011806	22.31645337022551
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10590.0
1	5982.5
2	1053.0
3	604.5
4	431.5
5	339.5
6	283.5
7	269.5
8	263.0
9	262.0
10	261.0
11	261.5
12	314.0
13	459.5
14	631.5
15	1209.5
16	18242.0
17	19979.5
18	4718.5
19	4063.5
20	4171.0
21	5127.5
22	6792.0
23	9085.0
24	12086.5
25	16178.0
26	21725.0
27	29242.5
28	38882.5
29	50446.5
30	64261.5
31	80272.5
32	98410.0
33	116831.5
34	132325.0
35	145407.0
36	156146.5
37	164802.5
38	170272.0
39	171648.0
40	172578.5
41	176502.0
42	184167.0
43	191140.0
44	193727.5
45	192405.5
46	187239.0
47	180629.5
48	175409.0
49	166977.0
50	155518.0
51	143219.0
52	126834.0
53	109672.5
54	96864.0
55	87339.5
56	77298.5
57	67236.0
58	59740.5
59	53368.5
60	45967.5
61	39209.5
62	33740.0
63	28658.5
64	24155.5
65	19903.0
66	16176.0
67	12763.0
68	9682.5
69	7259.5
70	5176.0
71	3503.0
72	2385.5
73	1599.5
74	1042.0
75	606.0
76	310.5
77	183.5
78	117.0
79	57.5
80	15.0
81	5.5
82	4.0
83	3.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.5
91	2.0
92	1.5
93	1.5
94	4.0
95	3.0
96	0.5
97	0.5
98	3.5
99	4.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0018657246637302474
76-77	0.0025707950308376084
78-79	0.006931384070486209
80-81	0.006475800140970684
82-83	0.0012257377151251045
84-85	4.1219498384738025E-4
86-87	0.0015294603348021216
88-89	0.0011715015330399227
90-91	1.4101407342147218E-4
92-93	7.593065491925426E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4609469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.37105073182086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.89637003306076	47.29326531349283
2	8.567429174337502	9.659099692557227
3	2.5564909037314414	4.323362352890508
4	1.254374057325018	2.8284153448859426
5	0.7652283960343564	2.156836436714131
6	0.5231072688787566	1.7692863835289188
7	0.3956964203833861	1.561407609148224
8	0.3102212410236753	1.398999785266722
9	0.21466466546637197	1.0890785472600788
>10	1.3675851993441774	14.50089402404272
>50	0.12523599975429991	4.724353434331539
>100	0.02088387517696837	1.8759292205446623
>500	9.300913272369869E-4	0.3476342041232502
>1k	0.0013176287240062332	1.4994600075750895
>5k	2.712765020012684E-4	1.1381503967867983
>10k+	1.9376893000090602E-4	3.8338272468512327
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	126329	2.740641058655563	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	13941	0.30244264577980673	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13103	0.28426267754485385	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	11374	0.24675293401474227	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	10747	0.23315049954777872	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAC	9421	0.2043836285697984	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	9118	0.19781020330107438	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAT	7727	0.16763319158887932	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	7545	0.1636847975330781	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	7144	0.15498531392661496	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	6059	0.13144681090164614	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5085	0.1103163943612594	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAG	4654	0.10096607656977408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.423618208518161E-4	0.0	0.0	0.0	0.0
2	5.423618208518161E-4	0.0	0.0	0.0	0.0
3	5.857507665199614E-4	0.0	0.0	2.1694472834072646E-5	0.0
4	6.291397121881067E-4	0.0	0.0	2.1694472834072646E-5	0.0
5	7.159176035243973E-4	0.0	0.0	6.508341850221793E-5	0.0
6	7.810010220266153E-4	0.0	0.0	6.508341850221793E-5	0.0
7	8.677789133629057E-4	0.0	0.0	6.508341850221793E-5	0.0
8	0.0010196402232014142	0.0	0.0	6.508341850221793E-5	0.0
9	0.0012148904787080681	0.0	0.0	6.508341850221793E-5	0.0
10-11	0.0014101407342147218	0.0	0.0	6.508341850221793E-5	0.0
12-13	0.0016921688810576665	0.0	0.0	6.508341850221793E-5	0.0
14-15	0.0020067387371517195	1.0847236417036323E-5	0.0	6.508341850221793E-5	0.0
16-17	0.002288766883994664	2.1694472834072646E-5	0.0	6.508341850221793E-5	0.0
18-19	0.0027443508135101893	3.254170925110897E-5	0.0	6.508341850221793E-5	0.0
20-21	0.002993837251102025	4.338894566814529E-5	0.0	6.508341850221793E-5	0.0
22-23	0.0033084071071960786	5.423618208518161E-5	0.0	6.508341850221793E-5	0.0
24-25	0.0036555186725412405	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
26-27	0.004013477474303439	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
28-29	0.004273811148312311	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
30-31	0.004664311659325618	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
32-33	0.005043964933921889	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
34-35	0.005325993080764834	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
36-37	0.005727340828195178	6.508341850221793E-5	0.0	6.508341850221793E-5	0.0
38-39	0.00607445239354034	6.508341850221793E-5	0.0	8.677789133629058E-5	0.0
40-41	0.00631309159471514	6.508341850221793E-5	0.0	8.677789133629058E-5	0.0
42-43	0.006540883559472903	6.508341850221793E-5	0.0	8.677789133629058E-5	0.0
44-45	0.0069530785433202826	6.508341850221793E-5	0.0	9.762512775332691E-5	0.0
46-47	0.007278495635831372	6.508341850221793E-5	0.0	1.0847236417036322E-4	0.0
48-49	0.007549676546257281	6.508341850221793E-5	0.0	1.0847236417036322E-4	0.0
50-51	0.007701537856095789	6.508341850221793E-5	0.0	1.0847236417036322E-4	1.0847236417036323E-5
52-53	0.008688636370046094	6.508341850221793E-5	0.0	1.0847236417036322E-4	2.1694472834072646E-5
54-55	0.009979457503673416	6.508341850221793E-5	0.0	1.0847236417036322E-4	4.338894566814529E-5
56-57	0.011715015330399228	6.508341850221793E-5	0.0	1.0847236417036322E-4	4.338894566814529E-5
58-59	0.01336379526578875	6.508341850221793E-5	0.0	1.0847236417036322E-4	4.338894566814529E-5
60-61	0.01521867269310196	7.593065491925426E-5	0.0	1.0847236417036322E-4	4.338894566814529E-5
62-63	0.01748574510426255	8.677789133629058E-5	0.0	1.0847236417036322E-4	4.338894566814529E-5
64-65	0.020230095917772743	8.677789133629058E-5	0.0	1.1931960058739954E-4	4.338894566814529E-5
66-67	0.023766294989726582	8.677789133629058E-5	0.0	1.5186130983850852E-4	4.338894566814529E-5
68-69	0.028267898102796656	9.762512775332691E-5	0.0	1.5186130983850852E-4	4.338894566814529E-5
70-71	0.03364812736564667	1.0847236417036322E-4	0.0	1.5186130983850852E-4	4.338894566814529E-5
72-73	0.04086153958297582	1.0847236417036322E-4	0.0	1.5186130983850852E-4	4.338894566814529E-5
74-75	0.05046134381205297	1.0847236417036322E-4	0.0	1.5186130983850852E-4	4.338894566814529E-5
76-77	0.06199195612336258	1.0847236417036322E-4	0.0	1.5186130983850852E-4	4.338894566814529E-5
78-79	0.07618014135684609	1.3016683700443587E-4	0.0	1.5186130983850852E-4	4.338894566814529E-5
80-81	0.09510856890457448	1.3016683700443587E-4	0.0	1.5186130983850852E-4	5.423618208518161E-5
82-83	0.11751895934217152	1.3016683700443587E-4	0.0	1.5186130983850852E-4	6.508341850221793E-5
84-85	0.14754410974452806	1.3016683700443587E-4	0.0	1.5186130983850852E-4	6.508341850221793E-5
86-87	0.18406675476068934	1.3016683700443587E-4	0.0	1.5186130983850852E-4	6.508341850221793E-5
88-89	0.23002649545967224	1.3016683700443587E-4	0.0	1.5186130983850852E-4	6.508341850221793E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	90260	0.0	94.15162	1
TCGACGG	92555	0.0	91.82729	2
ATATCTC	40935	0.0	90.10216	1
CGACGGC	94845	0.0	89.535034	3
TATCTCG	41425	0.0	88.82997	2
GACGGCG	95775	0.0	88.65571	4
ACGGCGC	95895	0.0	88.515045	5
CGGCGCG	96865	0.0	87.62376	6
ATCTCGA	43935	0.0	83.67945	3
TCTCGAG	42985	0.0	82.35741	4
CTCGAGG	44675	0.0	79.42268	5
TCGAGGG	45435	0.0	78.0837	6
AGGGCGC	46760	0.0	76.25712	9
CGAGGGC	47330	0.0	75.18821	7
GAGGGCG	48010	0.0	74.2321	8
CTCGACG	2030	0.0	64.81451	5
CGCGCCG	143810	0.0	59.99443	9
GGCGCGC	143945	0.0	59.948063	7
GCGCGCC	144000	0.0	59.921864	8
TCTCGAC	2230	0.0	59.214546	4
>>END_MODULE