FastQCFastQC Report
Thu 26 May 2016
SRR1051294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609469
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460450.9989220016448749No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT139540.3027246739266497No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT72970.1583045682702281No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49490.10736594605582551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG31500.088.356141
TCGACGG35550.078.563712
ATCTCGA86350.055.0420531
TCTCGAG96100.049.5254672
CGACGGC57500.048.4902573
GACGGCG60400.046.083444
ACGGCGC62550.044.6513145
TATCTCG41900.044.2114562
ATATCTC44150.043.5353551
CTCGAGG119850.039.8698123
TCGAGGG124200.038.588144
CGGCGCG75800.036.8461726
AGGGCGC137850.034.732667
CGAGGGC138550.034.625745
GGGCGCG138600.034.5789768
GAGGGCG150050.031.9403296
CGCGCCG213800.022.9274759
GCGCGCC217450.022.5426278
GGCGCGC218150.022.4702937
ATCCATA93750.017.96094516-17
Produced by FastQC (version 0.11.5)