FastQCFastQC Report
Thu 26 May 2016
SRR1051293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14267472
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3202872.244875616367076No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT668650.46865345171169775No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA207290.14528852763825295No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA153680.10771354588955913No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT142750.10005276337672153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1276000.094.639861
TCGACGG1289850.093.631012
CGACGGC1308350.092.161663
ACGGCGC1329950.090.648025
GACGGCG1331650.090.607524
CGGCGCG1341950.089.8301856
ATATCTC631650.086.59211
TATCTCG631100.086.441742
ATCTCGA663350.082.168133
TCTCGAG674650.080.184344
CTCGAGG692050.078.161155
TCGAGGG714750.075.666066
AGGGCGC714850.075.5531549
CGAGGGC751150.071.8460857
GAGGGCG759850.071.153748
CGCGCCG2047700.059.5078939
GGCGCGC2062500.059.187647
GCGCGCC2066200.058.991178
ATCCATA1428100.042.22074516-17
TCCATAT1445200.041.59464318-19
Produced by FastQC (version 0.11.5)