##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051293_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14267472 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40694700504756 34.0 31.0 34.0 31.0 34.0 2 32.57002298655291 34.0 31.0 34.0 31.0 34.0 3 32.64097620096959 34.0 31.0 34.0 31.0 34.0 4 36.13320881232499 37.0 37.0 37.0 35.0 37.0 5 36.09340701702446 37.0 37.0 37.0 35.0 37.0 6 36.13696631049986 37.0 37.0 37.0 35.0 37.0 7 36.08297012953661 37.0 37.0 37.0 35.0 37.0 8 36.08002566957902 37.0 37.0 37.0 35.0 37.0 9 37.77471068455575 39.0 38.0 39.0 35.0 39.0 10-11 37.81312838742561 39.0 38.0 39.0 35.0 39.0 12-13 37.748810090533205 39.0 38.0 39.0 35.0 39.0 14-15 39.26805989175938 41.0 39.0 41.0 36.0 41.0 16-17 39.2231298929481 41.0 39.0 41.0 36.0 41.0 18-19 39.21460178789907 41.0 39.0 41.0 36.0 41.0 20-21 39.24830222200541 41.0 39.0 41.0 36.0 41.0 22-23 39.125711198171615 41.0 39.0 41.0 36.0 41.0 24-25 39.079673680102545 41.0 39.0 41.0 36.0 41.0 26-27 39.05506483559246 41.0 39.0 41.0 35.5 41.0 28-29 38.88645143302191 40.5 39.0 41.0 35.0 41.0 30-31 38.41899293722111 40.0 37.0 41.0 35.0 41.0 32-33 38.156471798227464 40.0 36.5 41.0 35.0 41.0 34-35 38.03126377258704 40.0 35.0 41.0 34.5 41.0 36-37 37.92484453447675 40.0 35.0 41.0 34.0 41.0 38-39 37.61412126829477 40.0 35.0 41.0 33.5 41.0 40-41 36.63096587818781 40.0 35.0 41.0 29.5 41.0 42-43 36.63006333567713 40.0 35.0 41.0 29.0 41.0 44-45 36.53012621997786 40.0 35.0 41.0 29.0 41.0 46-47 36.6344159287644 40.0 35.0 41.0 29.0 41.0 48-49 36.65687165883347 40.0 35.0 41.0 29.0 41.0 50-51 36.26698300161374 39.5 35.0 40.5 28.5 41.0 52-53 36.14006615888225 39.0 35.0 40.5 29.0 41.0 54-55 36.044604082629355 39.0 35.0 41.0 28.0 41.0 56-57 35.709768836413346 39.0 35.0 41.0 27.0 41.0 58-59 35.36433942186815 39.0 35.0 41.0 26.0 41.0 60-61 34.99733533733236 38.0 34.5 41.0 25.5 41.0 62-63 34.769032698995304 37.5 34.0 41.0 25.0 41.0 64-65 34.488593949930305 37.0 34.0 40.0 25.0 41.0 66-67 34.14927413209573 37.0 34.0 40.0 25.0 41.0 68-69 33.85381485241394 36.0 34.0 39.0 25.0 41.0 70-71 33.51819337721497 36.0 34.0 39.0 24.5 41.0 72-73 33.14627780590703 35.0 34.0 39.0 24.0 41.0 74-75 32.79199966188824 35.0 34.0 37.0 23.0 40.0 76-77 32.43308758552321 35.0 34.0 37.0 21.5 39.0 78-79 32.110222679953395 35.0 33.0 36.5 20.0 39.0 80-81 31.79511282727592 35.0 33.0 36.0 19.5 38.0 82-83 31.53680003717547 35.0 33.0 36.0 19.0 37.0 84-85 31.30330113842172 35.0 33.0 35.0 18.5 37.0 86-87 31.094332303578376 35.0 33.0 35.0 18.0 36.5 88-89 30.89162403823186 35.0 33.0 35.0 16.0 36.0 90-91 30.691819896334824 35.0 33.0 35.0 9.5 36.0 92-93 30.490633939915917 35.0 33.0 35.0 6.0 36.0 94-95 30.307308330445647 35.0 32.0 35.0 2.0 35.0 96-97 30.094625277694604 35.0 32.0 35.0 2.0 35.0 98-99 29.849229947673983 35.0 32.0 35.0 2.0 35.0 100-101 28.95787876787142 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39269293084853274 1101 2 0.4384946201758737 1101 3 0.42798466242376065 1101 4 0.3007549880989586 1101 5 0.3082221213813412 1101 6 0.30253827481233486 1101 7 0.3564439132276078 1101 8 0.2752380008480557 1101 9 0.451365163074378 1101 10-11 0.39022194925468057 1101 12-13 0.4278578246453719 1101 14-15 0.5448112641593355 1101 16-17 0.4741392682521024 1101 18-19 0.5651756752542241 1101 20-21 0.5563193585032877 1101 22-23 0.622048110476527 1101 24-25 0.6352638100997581 1101 26-27 0.5827461451635116 1101 28-29 0.5771026520366647 1101 30-31 0.5974159799262182 1101 32-33 0.5603654174059827 1101 34-35 0.5080998727690584 1101 36-37 0.5137852118302746 1101 38-39 0.45754477158903484 1101 40-41 0.34221359203145596 1101 42-43 0.3265102140932683 1101 44-45 0.30369469374778646 1101 46-47 0.34749121679207917 1101 48-49 0.33275676349307304 1101 50-51 0.26247104179994807 1101 52-53 0.18730110859418403 1101 54-55 0.15967295578870022 1101 56-57 0.21628027034225994 1101 58-59 0.14678851009831817 1101 60-61 0.1528615572191967 1101 62-63 0.2336706905758703 1101 64-65 0.18641808801319826 1101 66-67 0.2482271537171883 1101 68-69 0.14632675889927782 1101 70-71 0.10917969474269285 1101 72-73 0.15300131181707854 1101 74-75 0.1309167277874792 1101 76-77 0.08155148343902141 1101 78-79 0.042884689778404095 1101 80-81 0.054236918725720784 1101 82-83 0.0675555377654149 1101 84-85 0.0947611228109082 1101 86-87 0.0622907242820574 1101 88-89 0.028843653384655 1101 90-91 0.0017457573668551163 1101 92-93 0.02980739586000425 1101 94-95 0.10314258559269973 1101 96-97 0.09356406840234399 1101 98-99 0.046545084667982906 1101 100-101 0.3448048868741367 1102 1 0.31138759475913247 1102 2 0.33727623350842606 1102 3 0.3383737634607158 1102 4 0.1667261320239959 1102 5 0.24598934929417027 1102 6 0.2366108567542753 1102 7 0.28362232294715284 1102 8 0.2529692469437066 1102 9 0.30466803520958763 1102 10-11 0.31716884640817966 1102 12-13 0.3053287742307944 1102 14-15 0.4550258030204972 1102 16-17 0.45924731959171794 1102 18-19 0.4598244869603292 1102 20-21 0.4851230188701834 1102 22-23 0.48536859044178726 1102 24-25 0.47578001379707757 1102 26-27 0.4280607850077587 1102 28-29 0.47258705791771405 1102 30-31 0.4579241962937033 1102 32-33 0.4094200857770076 1102 34-35 0.4111682028235819 1102 36-37 0.40639182492983394 1102 38-39 0.35204476570392984 1102 40-41 0.2559816356106097 1102 42-43 0.2565172355226082 1102 44-45 0.23656349621626305 1102 46-47 0.19304338283542677 1102 48-49 0.22031412901270642 1102 50-51 0.15354310920024972 1102 52-53 0.1086556360378168 1102 54-55 0.23702405057729692 1102 56-57 0.24273822658607003 1102 58-59 0.22430970127700078 1102 60-61 0.1342793572710974 1102 62-63 0.16871522219857837 1102 64-65 0.2625793066072717 1102 66-67 0.27982352558051815 1102 68-69 0.14350539144319896 1102 70-71 0.19391345526439352 1102 72-73 0.1897396106709408 1102 74-75 0.22206255530954877 1102 76-77 0.18721099417223996 1102 78-79 0.18159657439313293 1102 80-81 0.16282496756776865 1102 82-83 0.1743730625564588 1102 84-85 0.191729607457642 1102 86-87 0.1592193040022778 1102 88-89 0.12955333974987582 1102 90-91 0.1254385592663283 1102 92-93 0.164099692270824 1102 94-95 0.14120304962924024 1102 96-97 0.15319435943133541 1102 98-99 0.19408592333477515 1102 100-101 0.42538957969874147 1103 1 0.2748333683385127 1103 2 0.22282620637241735 1103 3 0.22988247854664934 1103 4 0.18121801091541556 1103 5 0.22642929936657197 1103 6 0.24828013741082344 1103 7 0.26315118816275174 1103 8 0.20037502381563144 1103 9 0.3263065635123965 1103 10-11 0.2628615802018004 1103 12-13 0.27192881021335324 1103 14-15 0.4160806141176039 1103 16-17 0.3997839476581788 1103 18-19 0.42131859878966793 1103 20-21 0.3827809120522403 1103 22-23 0.4444905848092944 1103 24-25 0.455846096030065 1103 26-27 0.4352711765437647 1103 28-29 0.42397206837497237 1103 30-31 0.412543948573159 1103 32-33 0.379476388619878 1103 34-35 0.3196400576655165 1103 36-37 0.3686877614558526 1103 38-39 0.39081858738668984 1103 40-41 0.2349579014226464 1103 42-43 0.220219837936952 1103 44-45 0.24088967805560912 1103 46-47 0.21606075799180502 1103 48-49 0.2648036671076426 1103 50-51 0.11480814159987318 1103 52-53 0.0860629885680737 1103 54-55 0.1210496550202862 1103 56-57 0.15459965387275076 1103 58-59 0.16686841846967582 1103 60-61 0.22540114955904045 1103 62-63 0.2547216215399146 1103 64-65 0.20643569166276166 1103 66-67 0.26424681737898936 1103 68-69 0.21137537094994485 1103 70-71 0.1536154658574418 1103 72-73 0.20336572614010606 1103 74-75 0.10383740979281697 1103 76-77 0.1866456804212575 1103 78-79 0.1430317640978629 1103 80-81 0.14237253199145172 1103 82-83 0.09478146967715873 1103 84-85 0.07562727055419671 1103 86-87 0.11104926501162282 1103 88-89 0.10645798937910911 1103 90-91 0.09847263829495034 1103 92-93 0.0945900591829556 1103 94-95 0.11455301805725782 1103 96-97 0.15226851967306132 1103 98-99 0.20406449560037387 1103 100-101 0.4920361483415512 1104 1 0.19121318869930803 1104 2 0.18152938651729755 1104 3 0.14015374130602964 1104 4 0.13335587837234897 1104 5 0.15852527920824144 1104 6 0.16620206300040508 1104 7 0.17659438948935957 1104 8 0.1596861304067474 1104 9 0.24571437752865677 1104 10-11 0.2234170520775578 1104 12-13 0.2359443268496335 1104 14-15 0.3053735454053452 1104 16-17 0.2978223955447419 1104 18-19 0.3163647873216462 1104 20-21 0.3287770371388632 1104 22-23 0.3698232857215018 1104 24-25 0.3800529221025357 1104 26-27 0.296409848490768 1104 28-29 0.3242495727518033 1104 30-31 0.3126988106262871 1104 32-33 0.31843742289235877 1104 34-35 0.30252237732722875 1104 36-37 0.29291301224703403 1104 38-39 0.2503378072053266 1104 40-41 0.15386001690355045 1104 42-43 0.0382982612141376 1104 44-45 0.13478439656497443 1104 46-47 0.14841280314284688 1104 48-49 0.17927930330484543 1104 50-51 0.04148593019761648 1104 52-53 -0.007771652678698615 1104 54-55 0.07493387623643599 1104 56-57 0.214658826134702 1104 58-59 0.10195118941156522 1104 60-61 0.08585585243119453 1104 62-63 0.18204317680006454 1104 64-65 0.1948860499871614 1104 66-67 0.1887971735953613 1104 68-69 0.15108116815234496 1104 70-71 0.1233710366423324 1104 72-73 0.11521269650596366 1104 74-75 0.13494975114178942 1104 76-77 0.15573489886777736 1104 78-79 0.1503322301741008 1104 80-81 0.1570803477877014 1104 82-83 0.0753429783604922 1104 84-85 0.09874810917706611 1104 86-87 0.06515288064259295 1104 88-89 0.048198558227539934 1104 90-91 0.03122405817546081 1104 92-93 0.04282646483780894 1104 94-95 0.0670934467803832 1104 96-97 0.11953498615904934 1104 98-99 0.15012559214315857 1104 100-101 0.4229973171203696 1105 1 0.07934458081241047 1105 2 0.027484559007532994 1105 3 0.12399535219768154 1105 4 0.06346613005592161 1105 5 0.07627360126254246 1105 6 0.11554624487533971 1105 7 0.14058736435008967 1105 8 0.11266559888818506 1105 9 0.15589011083178406 1105 10-11 0.0663508960323469 1105 12-13 0.15519766343950891 1105 14-15 0.21257244039237833 1105 16-17 0.23873504745659346 1105 18-19 0.2497234644335009 1105 20-21 0.16136553329378955 1105 22-23 0.21578525099967294 1105 24-25 0.22179076451435265 1105 26-27 0.18927677960512312 1105 28-29 0.23618574707428763 1105 30-31 0.2640232212772986 1105 32-33 0.21020248835827005 1105 34-35 0.16057604689052596 1105 36-37 0.21660925619273286 1105 38-39 0.15115388268817753 1105 40-41 0.07487061214916224 1105 42-43 0.1056458387446284 1105 44-45 0.09702606548881221 1105 46-47 0.05984849560545058 1105 48-49 0.09737931600166405 1105 50-51 -0.0018969523545493416 1105 52-53 0.0047431507966848585 1105 54-55 0.11374532755096567 1105 56-57 0.13913057691482322 1105 58-59 0.09379264979708068 1105 60-61 0.06815943460724583 1105 62-63 0.06912675662723444 1105 64-65 0.1145342819191626 1105 66-67 0.11697629506298313 1105 68-69 0.10351128362101747 1105 70-71 0.10601701118028473 1105 72-73 0.1184080597997621 1105 74-75 0.12939084812198587 1105 76-77 0.13006878708725367 1105 78-79 0.09634737075530353 1105 80-81 0.08944223209605084 1105 82-83 0.08745031622430943 1105 84-85 0.072309294359588 1105 86-87 0.032328667255328014 1105 88-89 0.0019684808872995063 1105 90-91 -0.03455807335280525 1105 92-93 0.0070923083173859425 1105 94-95 0.028148843177923766 1105 96-97 0.09516782451528982 1105 98-99 0.13921282449440753 1105 100-101 0.42076712364726987 1106 1 0.030150768532571703 1106 2 -0.006194756042660288 1106 3 -0.00434236197675375 1106 4 0.012603500831332326 1106 5 -0.010268640857347577 1106 6 0.02511499592893074 1106 7 0.04578866311581464 1106 8 0.05189175185527972 1106 9 -0.014044953757803569 1106 10-11 0.05201870704286904 1106 12-13 0.0364354502442481 1106 14-15 0.11045727518253301 1106 16-17 0.06559262865233251 1106 18-19 0.08601613066153391 1106 20-21 0.046508030417783175 1106 22-23 0.09490170862914482 1106 24-25 0.09538794232889103 1106 26-27 0.078277518849589 1106 28-29 0.08923397535843236 1106 30-31 0.07070730961410732 1106 32-33 0.08162415665228195 1106 34-35 0.0272326895242756 1106 36-37 0.06462939026329906 1106 38-39 0.06616587773610405 1106 40-41 0.05763417930783987 1106 42-43 0.005175014360268904 1106 44-45 0.05054040531206283 1106 46-47 -0.06103992377503431 1106 48-49 -0.03950580508215751 1106 50-51 -0.18820719019782217 1106 52-53 -0.0983735138001478 1106 54-55 -0.033945309975123905 1106 56-57 -0.040473561339567254 1106 58-59 -0.02936005027140709 1106 60-61 -0.012524991309724953 1106 62-63 -0.0394374681776668 1106 64-65 0.005333565374961324 1106 66-67 0.058590987023123375 1106 68-69 0.03288829820358785 1106 70-71 0.026300041443199973 1106 72-73 0.03195830112667153 1106 74-75 0.042040134355403325 1106 76-77 0.022716696973340333 1106 78-79 0.03535142085443965 1106 80-81 0.022178499981794886 1106 82-83 0.034094700906941 1106 84-85 6.768182088876529E-4 1106 86-87 -0.0733802299877766 1106 88-89 -0.0158310141372624 1106 90-91 -0.03023878004041336 1106 92-93 0.017376206376518155 1106 94-95 -0.008930343358862558 1106 96-97 -0.03411503501632396 1106 98-99 -0.029385294690897013 1106 100-101 0.31338445935422143 1107 1 -0.062308019973855266 1107 2 -0.06010841033354808 1107 3 -0.0070045841534707165 1107 4 -0.05500664492997487 1107 5 -0.12330511112288889 1107 6 -0.1224893542323855 1107 7 -0.03468112661563083 1107 8 -0.09759617052117164 1107 9 -0.10478770264318626 1107 10-11 -0.06464967809431954 1107 12-13 -0.07816483151843556 1107 14-15 -0.11568739792005545 1107 16-17 -0.07958468714679157 1107 18-19 0.00420356507213171 1107 20-21 -0.04051374339036329 1107 22-23 -0.04796189103631576 1107 24-25 -0.016721997548351908 1107 26-27 -0.10684083446431458 1107 28-29 -0.1193352172227975 1107 30-31 -0.0860186078330969 1107 32-33 -0.10903086713840082 1107 34-35 -0.12431486685939319 1107 36-37 -0.15141883642359488 1107 38-39 -0.31434530154063367 1107 40-41 -0.3402787520952941 1107 42-43 -0.26581338960896517 1107 44-45 -0.18901358078834107 1107 46-47 -0.3947055047140253 1107 48-49 -0.27021246231950613 1107 50-51 -0.2607756585617196 1107 52-53 -0.21539208799568144 1107 54-55 -0.12592624733952107 1107 56-57 -0.11753002764388043 1107 58-59 -0.13814656234087863 1107 60-61 -0.18609025899736054 1107 62-63 -0.25595304793554874 1107 64-65 -0.14430843766098178 1107 66-67 -0.08723047268716755 1107 68-69 -0.05982949405794358 1107 70-71 -0.1075205674746087 1107 72-73 -0.13181268177508798 1107 74-75 -0.0918361359444333 1107 76-77 -0.07893703341067493 1107 78-79 -0.171266446789339 1107 80-81 -0.15510743144185923 1107 82-83 -0.15018094814641003 1107 84-85 -0.1248568649434425 1107 86-87 -0.12623141411922134 1107 88-89 -0.17118960630593705 1107 90-91 -0.1936286635739819 1107 92-93 -0.16004432305954452 1107 94-95 -0.20381746809908208 1107 96-97 -0.21353938971679653 1107 98-99 -0.20705207618818022 1107 100-101 0.12144948415138757 1108 1 -0.4172887553157416 1108 2 -0.30022858613742187 1108 3 -0.2640281910801008 1108 4 -0.20191001252565144 1108 5 -0.17640564839536665 1108 6 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-0.838889259467706 2308 46-47 -0.953507836564242 2308 48-49 -0.705465502526522 2308 50-51 -1.1754412116489803 2308 52-53 -1.0915246441748465 2308 54-55 -0.7628880879973607 2308 56-57 -0.7967529196195358 2308 58-59 -1.0556185857943987 2308 60-61 -1.070272868350024 2308 62-63 -1.0254696976332198 2308 64-65 -1.182719697488551 2308 66-67 -0.9257776916449529 2308 68-69 -0.8916346641085511 2308 70-71 -0.8002127163548494 2308 72-73 -0.8195535639096576 2308 74-75 -1.0508563645010938 2308 76-77 -0.9292295112054703 2308 78-79 -0.8392177320463396 2308 80-81 -0.904876519539684 2308 82-83 -0.8722291148679346 2308 84-85 -0.8430089555587692 2308 86-87 -0.7996443936962123 2308 88-89 -0.9113193342652472 2308 90-91 -0.9800295038761817 2308 92-93 -0.9776042036932608 2308 94-95 -0.9066949357240048 2308 96-97 -0.9613270138455512 2308 98-99 -0.994699446764308 2308 100-101 -0.52436851298161 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 116.0 11 871.0 12 2839.0 13 6074.0 14 9688.0 15 13780.0 16 19509.0 17 33114.0 18 81256.0 19 236409.0 20 254212.0 21 157623.0 22 127932.0 23 117339.0 24 118970.0 25 133580.0 26 158976.0 27 182397.0 28 200437.0 29 218519.0 30 239637.0 31 272769.0 32 326210.0 33 412250.0 34 572061.0 35 873547.0 36 1592143.0 37 3105271.0 38 3783021.0 39 1015731.0 40 1191.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.190569755338114 31.287924947076007 20.39769795794664 19.12380733963924 2 20.755567990050036 26.028405093117218 35.57572507828088 17.64030183855186 3 20.29693990001022 27.703383651588197 23.617135842185323 28.382540606216256 4 29.723472053967775 23.493418573061657 27.336152648142125 19.44695672482844 5 20.909105693154117 33.12504521246916 22.8218735262137 23.143975568163018 6 20.904029685038044 23.49994252519079 25.765312130268757 29.83071565950241 7 29.930766138166614 23.49825783386004 22.783588424884435 23.78738760308891 8 33.03477308383714 23.629021315058477 22.90754101357269 20.428664587531696 9 20.335235282045762 26.98363101746406 23.20045204924881 29.48068165124137 10-11 28.895400281682708 23.431874386432103 23.08985512955116 24.58287020233403 12-13 26.696702120740103 23.29048551838756 23.369651610320314 26.643160750552024 14-15 26.532272150244978 23.266118903194624 23.47667827909527 26.724930667465124 16-17 26.51425564388702 27.782837071626986 23.327787151080443 22.375120133405556 18-19 22.165492947874718 23.335693246848496 27.898807160792042 26.600006644484743 20-21 21.84545727512204 29.748013523348778 23.773062249570213 24.633466951958972 22-23 19.865880935319165 27.575589424671733 30.29265801257574 22.265871627433366 24-25 19.86573725184111 23.131343800779845 34.98581949205858 22.017099455320466 26-27 19.83601579873435 23.138117951098835 37.11649828364828 19.909367966518527 28-29 19.85966399653702 23.069552896266416 37.250547959722645 19.82023514747392 30-31 19.900347447676786 22.96146787601896 37.20124349989963 19.93694117640462 32-33 19.89428528513437 23.000399302996538 37.17965431729105 19.925661094578036 34-35 19.935569518086616 23.078488229570898 37.157592160432365 19.828350091910124 36-37 19.95047896361738 23.015009246207036 37.02909667529048 20.005415114885103 38-39 20.176744694930907 23.224661817692212 36.22348420426951 20.37510928310737 40-41 21.518128789739347 24.407568488657276 32.17824783535583 21.896054886247544 42-43 21.70976556514991 24.662391611432426 32.42323873878231 21.204604084635356 44-45 21.93796833197943 25.35998174858892 31.539111217959277 21.162938701472378 46-47 21.655360529181344 26.12545516122268 31.13184311838846 21.087341191207525 48-49 21.5380552350129 26.58354962953493 30.52361343340993 21.35478170204224 50-51 21.620831123902786 26.65621606455734 30.17613987429209 21.54681293724778 52-53 21.671685707246656 26.580146187076988 30.11380892086714 21.634359184809217 54-55 21.78096875127476 26.23786761958978 30.06978567500535 21.91137795413011 56-57 21.794849420099272 26.12881131740364 30.131455940034662 21.94488332246243 58-59 21.92791347870809 26.163944305435084 29.989130133472937 21.919012082383897 60-61 22.102352223316387 26.19539819527848 29.59093967148771 22.111309909917424 62-63 22.284258661378356 26.38572450750839 29.178187880981625 22.151828950131623 64-65 22.39355174781395 26.58252699107638 28.755417630291575 22.26850363081809 66-67 22.4836945180959 26.613436450274037 28.402660326228098 22.50020870540197 68-69 22.51819032832165 26.76692829675783 28.22654917423353 22.48833220068699 70-71 22.66311439055216 26.97482076712679 27.97783307372182 22.384231768599232 72-73 22.75105568807144 26.991498564006296 27.7655389826593 22.49190676526297 74-75 22.79442802722478 27.043283834256638 27.705360984352374 22.456927154166216 76-77 22.88001750020186 27.178644763284677 27.51514586118263 22.42619187533083 78-79 22.873893931461243 27.084644433492056 27.47865700960976 22.562804625436943 80-81 22.859997970160233 27.02691696497461 27.57150418976871 22.541580875096447 82-83 22.882654387937038 27.10898381849259 27.536745982449062 22.47161581112131 84-85 22.84479720884896 26.981479529994846 27.608413334323473 22.565309926832725 86-87 22.820267098425848 27.034590674246985 27.626416162232108 22.518726065095056 88-89 22.898762074308625 27.07599115816427 27.51794276273232 22.507304004794783 90-91 22.945161660561446 26.995756864758498 27.405309219685414 22.653772254994642 92-93 22.931623708166164 27.0618471614334 27.41264685899763 22.59388227140281 94-95 23.001126899636823 27.051461282818025 27.348297497018393 22.59911432052676 96-97 22.99515289043497 26.979194352019753 27.244216775053072 22.781435982492205 98-99 22.999275547803894 26.961046396060595 27.293046099999636 22.746631956135875 100-101 23.086644801919565 27.031712340802404 27.12696087608326 22.754681981194764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5241.0 1 3025.0 2 630.0 3 388.5 4 318.5 5 285.5 6 259.5 7 264.5 8 284.5 9 300.5 10 325.0 11 362.0 12 519.5 13 997.5 14 1544.0 15 2645.5 16 32795.5 17 43450.0 18 24795.0 19 22825.0 20 20722.5 21 21947.0 22 26259.5 23 33658.5 24 43233.5 25 55606.0 26 71682.0 27 91330.0 28 114426.0 29 141027.5 30 170409.5 31 202972.5 32 240649.0 33 278973.0 34 311520.0 35 343595.0 36 378979.5 37 415299.5 38 445014.5 39 465945.5 40 483739.5 41 504174.0 42 533187.5 43 556762.5 44 569398.0 45 572124.0 46 563196.5 47 548033.5 48 537912.5 49 527203.0 50 509277.5 51 487998.5 52 447062.5 53 398839.5 54 366097.5 55 340771.0 56 309296.5 57 275631.5 58 251468.0 59 229877.5 60 202462.0 61 177329.5 62 156373.0 63 136665.5 64 118617.5 65 99565.5 66 81376.0 67 65070.5 68 50933.0 69 39765.0 70 29922.5 71 21991.0 72 16683.0 73 13143.5 74 10318.5 75 7462.0 76 5296.5 77 4106.5 78 3106.5 79 1898.0 80 831.0 81 317.0 82 118.5 83 56.0 84 31.5 85 16.5 86 9.0 87 4.5 88 5.0 89 7.0 90 6.0 91 4.5 92 4.5 93 3.5 94 2.5 95 2.0 96 2.0 97 2.0 98 3.0 99 3.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0021938013966314425 2 0.0174873306217107 3 0.005705285421271547 4 0.006202920881849286 5 0.010485389422877437 6 0.0024671504524417503 7 0.0012335752262208751 8 0.0 9 0.0 10-11 3.504475074491122E-6 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 7.779934665370292E-4 34-35 1.0513425223473367E-5 36-37 0.0 38-39 3.9600568341749675E-4 40-41 0.0 42-43 3.784833080450412E-4 44-45 5.747339122165441E-4 46-47 0.0 48-49 0.0 50-51 2.2779087984192294E-4 52-53 9.602261704105675E-4 54-55 5.186623110246861E-4 56-57 2.1026850446946734E-4 58-59 3.329251320766566E-4 60-61 0.0027720397839224777 62-63 0.007261272354345606 64-65 0.0011494678244330881 66-67 0.006665511591682115 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.114084401216978E-4 76-77 0.001457861630988307 78-79 0.00468548317459463 80-81 0.0023550072500580343 82-83 0.0013877721294984842 84-85 6.763636893767866E-4 86-87 0.0018188225636608924 88-89 0.002095676094545691 90-91 0.0021692700711100047 92-93 0.004492737045497619 94-95 5.607160119185795E-5 96-97 0.0 98-99 9.812530208575144E-4 100-101 4.275459590879169E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4267472E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.84580619623986 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2421976144285 37.43561023427848 2 12.152397226146448 11.628824850038518 3 3.7321050865936645 5.356967300314844 4 1.635871763913303 3.1307841351238785 5 0.9512643314375574 2.2757004421678526 6 0.5525439250055316 1.5862145730434616 7 0.3789035964311898 1.2690263629333494 8 0.2672627198873739 1.0229920239368986 9 0.21347538121118662 0.9192511545388974 >10 1.5507268406887704 15.367229930482196 >50 0.21001214179416539 6.911139631984758 >100 0.11138295341514232 8.531150103630889 >500 9.457345385542487E-4 0.3232814508997781 >1k 7.083152581366769E-4 0.6984473897780411 >5k 8.672967879101708E-5 0.28162300854328576 >10k+ 1.156395717213561E-4 3.2617574083049083 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 320287 2.244875616367076 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 66865 0.46865345171169775 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 20729 0.14528852763825295 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 15368 0.10771354588955913 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14275 0.10005276337672153 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.5044750744911223E-4 0.0 0.0 7.008950148982244E-6 0.0 2 3.574564575980945E-4 0.0 0.0 7.008950148982244E-6 0.0 3 3.7147435789605893E-4 7.008950148982244E-6 0.0 7.008950148982244E-6 0.0 4 3.8549225819402343E-4 7.008950148982244E-6 0.0 7.008950148982244E-6 0.0 5 3.9951015849198793E-4 7.008950148982244E-6 0.0 7.008950148982244E-6 0.0 6 4.2754595908791693E-4 1.4017900297964488E-5 0.0 7.008950148982244E-6 0.0 7 4.485728095348636E-4 1.4017900297964488E-5 0.0 7.008950148982244E-6 0.0 8 4.695996599818104E-4 1.4017900297964488E-5 0.0 7.008950148982244E-6 7.008950148982244E-6 9 5.396891614716328E-4 2.8035800595928976E-5 0.0 7.008950148982244E-6 7.008950148982244E-6 10-11 5.782383872910352E-4 2.8035800595928976E-5 0.0 7.008950148982244E-6 7.008950148982244E-6 12-13 6.518323638553487E-4 3.854922581940235E-5 0.0 7.008950148982244E-6 1.0513425223473367E-5 14-15 8.200471674309226E-4 4.5558175968384585E-5 0.0 1.4017900297964488E-5 1.4017900297964488E-5 16-17 0.0010933962232412301 4.906265104287571E-5 0.0 1.4017900297964488E-5 1.4017900297964488E-5 18-19 0.0011775036250290171 4.906265104287571E-5 0.0 1.4017900297964488E-5 1.4017900297964488E-5 20-21 0.001261611026816804 5.957607626634907E-5 0.0 1.4017900297964488E-5 1.4017900297964488E-5 22-23 0.001377258704275011 6.30805513408402E-5 0.0 1.4017900297964488E-5 1.4017900297964488E-5 24-25 0.0015700048333720227 7.008950148982245E-5 0.0 1.4017900297964488E-5 2.1026850446946733E-5 26-27 0.0017907867630649634 7.709845163880468E-5 0.0 1.4017900297964488E-5 2.4531325521437855E-5 28-29 0.001955497091566046 7.709845163880468E-5 0.0 2.1026850446946733E-5 2.8035800595928976E-5 30-31 0.0021938013966314425 9.111635193676917E-5 0.0 2.1026850446946733E-5 4.5558175968384585E-5 32-33 0.002460141502292768 9.812530208575142E-5 0.0 2.1026850446946733E-5 4.906265104287571E-5 34-35 0.002722977132879602 1.121432023837159E-4 0.0 2.1026850446946733E-5 4.906265104287571E-5 36-37 0.0030629112151052407 1.121432023837159E-4 0.0 2.1026850446946733E-5 5.607160119185795E-5 38-39 0.0033993408222563887 1.1564767745820703E-4 0.0 2.8035800595928976E-5 5.607160119185795E-5 40-41 0.0040266418605903 1.1915215253269816E-4 0.0 2.8035800595928976E-5 6.30805513408402E-5 42-43 0.004499745995646601 1.261611026816804E-4 0.0 2.8035800595928976E-5 6.30805513408402E-5 44-45 0.0049272919547345174 1.3317005283066264E-4 0.0 2.8035800595928976E-5 6.30805513408402E-5 46-47 0.005694771996048073 1.3667452790515377E-4 0.0 2.8035800595928976E-5 6.658502641533132E-5 48-49 0.006630466840937203 1.4718795312862714E-4 0.0 2.8035800595928976E-5 7.008950148982245E-5 50-51 0.007373415556729321 1.5419690327760937E-4 0.0 2.8035800595928976E-5 7.709845163880468E-5 52-53 0.008074310571627545 1.6471032850108274E-4 0.0 2.8035800595928976E-5 8.410740178778693E-5 54-55 0.008957438290399307 1.7872822879904724E-4 0.0 2.8035800595928976E-5 8.761187686227806E-5 56-57 0.010071861364087484 1.892416540225206E-4 0.0 3.15402756704201E-5 9.46208270112603E-5 58-59 0.010947980132710266 1.892416540225206E-4 0.0 3.504475074491122E-5 1.0162977716024254E-4 60-61 0.012339256737283241 1.9625060417150284E-4 0.0 3.504475074491122E-5 1.0513425223473367E-4 62-63 0.013698993066185796 1.9625060417150284E-4 0.0 3.504475074491122E-5 1.0513425223473367E-4 64-65 0.015395159002239499 1.9625060417150284E-4 0.0 4.5558175968384585E-5 1.0513425223473367E-4 66-67 0.017694094651105677 2.1026850446946734E-4 0.0 1.1564767745820703E-4 1.1564767745820703E-4 68-69 0.020774528241583372 2.1026850446946734E-4 0.0 1.2265662760718927E-4 1.1915215253269816E-4 70-71 0.02632561675957731 2.2779087984192294E-4 0.0 1.2966557775617152E-4 1.1915215253269816E-4 72-73 0.03252503316635211 2.383043050653963E-4 0.0 1.3317005283066264E-4 1.261611026816804E-4 74-75 0.039078401555650503 2.383043050653963E-4 0.0 1.401790029796449E-4 1.261611026816804E-4 76-77 0.047541708860546567 2.4531325521437853E-4 0.0 1.401790029796449E-4 1.3317005283066264E-4 78-79 0.05782033425402902 2.523222053633608E-4 0.0 1.401790029796449E-4 1.3317005283066264E-4 80-81 0.07099015158396665 2.5933115551234304E-4 0.0 1.6120585342659162E-4 1.3667452790515377E-4 82-83 0.08800437807062106 2.7334905581030754E-4 0.0 1.6120585342659162E-4 1.5069242820311827E-4 84-85 0.1085931691332564 2.8736695610827204E-4 0.0 1.6120585342659162E-4 1.5419690327760937E-4 86-87 0.13495383064357863 2.9087143118276316E-4 0.0 1.6120585342659162E-4 1.5419690327760937E-4 88-89 0.16714593867785407 2.943759062572543E-4 0.0 1.6120585342659162E-4 1.6821480357557387E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 127600 0.0 94.63986 1 TCGACGG 128985 0.0 93.63101 2 CGACGGC 130835 0.0 92.16166 3 ACGGCGC 132995 0.0 90.64802 5 GACGGCG 133165 0.0 90.60752 4 CGGCGCG 134195 0.0 89.830185 6 ATATCTC 63165 0.0 86.5921 1 TATCTCG 63110 0.0 86.44174 2 ATCTCGA 66335 0.0 82.16813 3 TCTCGAG 67465 0.0 80.18434 4 CTCGAGG 69205 0.0 78.16115 5 TCGAGGG 71475 0.0 75.66606 6 AGGGCGC 71485 0.0 75.553154 9 CGAGGGC 75115 0.0 71.846085 7 GAGGGCG 75985 0.0 71.15374 8 CGCGCCG 204770 0.0 59.507893 9 GGCGCGC 206250 0.0 59.18764 7 GCGCGCC 206620 0.0 58.99117 8 ATCCATA 142810 0.0 42.220745 16-17 TCCATAT 144520 0.0 41.594643 18-19 >>END_MODULE