FastQCFastQC Report
Thu 26 May 2016
SRR1051293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14267472
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277150.1942530533790429No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT165400.11592803546416632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG63850.086.820561
TCGACGG70400.079.014772
CGACGGC90700.061.3803183
GACGGCG110650.050.526964
ACGGCGC113800.049.211165
CGGCGCG126550.044.2146386
ATCTCGA92900.040.7363661
TCTCGAG108650.034.8459742
CTCGAGG125300.030.0629023
TATCTCG64950.027.9388312
AGGGCGC136800.027.7771917
TCGAGGG138850.027.5389984
CGCGCCG258600.026.9271189
GGGCGCG141050.026.805538
GGCGCGC274150.025.5730517
GCGCGCC279600.025.057598
ATATCTC84350.022.0382231
CGAGGGC178200.021.5908325
GAGGGCG188600.020.576116
ATCCATA183300.017.20637116-17
Produced by FastQC (version 0.11.5)