##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051293_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14267472 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55121131480055 34.0 31.0 34.0 31.0 34.0 2 32.80031739329855 34.0 31.0 34.0 31.0 34.0 3 32.88737738542609 34.0 31.0 34.0 31.0 34.0 4 36.32827763741187 37.0 37.0 37.0 35.0 37.0 5 36.2089149360167 37.0 37.0 37.0 35.0 37.0 6 36.17924906388462 37.0 37.0 37.0 35.0 37.0 7 36.155444075867116 37.0 37.0 37.0 35.0 37.0 8 36.139280035033536 37.0 36.0 37.0 35.0 37.0 9 37.87288617072457 39.0 38.0 39.0 35.0 39.0 10-11 37.86262727552575 39.0 38.0 39.0 35.0 39.0 12-13 37.85193729484803 39.0 38.0 39.0 35.0 39.0 14-15 39.32389266297491 41.0 39.0 41.0 36.0 41.0 16-17 39.313124532503025 41.0 39.0 41.0 36.0 41.0 18-19 39.36050436965988 41.0 39.0 41.0 36.0 41.0 20-21 39.386180747367156 41.0 39.0 41.0 36.0 41.0 22-23 39.34963397860532 41.0 39.0 41.0 36.0 41.0 24-25 39.301517956369565 41.0 39.0 41.0 36.0 41.0 26-27 39.18753914498658 40.5 39.0 41.0 36.0 41.0 28-29 39.05472759995604 40.0 39.0 41.0 35.5 41.0 30-31 38.94408129905564 40.0 38.0 41.0 35.0 41.0 32-33 38.89180290663966 40.0 38.0 41.0 35.0 41.0 34-35 38.8086611279139 40.0 38.0 41.0 35.0 41.0 36-37 38.72409257225107 40.0 38.0 41.0 35.0 41.0 38-39 38.623786014789445 40.0 38.0 41.0 35.0 41.0 40-41 38.64862710086272 40.0 38.0 41.0 35.0 41.0 42-43 38.63496041905672 40.0 38.0 41.0 35.0 41.0 44-45 38.56205591291857 40.0 38.0 41.0 34.5 41.0 46-47 38.447498232342774 40.0 38.0 41.0 34.0 41.0 48-49 38.36984961316202 40.0 38.0 41.0 34.0 41.0 50-51 38.32232500613984 40.0 37.5 41.0 34.0 41.0 52-53 38.1489299926434 40.0 37.0 41.0 34.0 41.0 54-55 37.96495563474735 40.0 37.0 41.0 33.0 41.0 56-57 37.76671291171975 40.0 36.0 41.0 33.0 41.0 58-59 37.53531396452013 39.0 36.0 41.0 33.0 41.0 60-61 37.24795920398512 39.0 35.0 41.0 33.0 41.0 62-63 36.93612095401343 39.0 35.0 41.0 32.5 41.0 64-65 36.628413043319796 38.0 35.0 40.0 32.0 41.0 66-67 36.25967122977357 37.0 35.0 40.0 32.0 41.0 68-69 35.88594282855435 37.0 35.0 39.5 31.0 41.0 70-71 35.558442133266496 36.0 35.0 39.0 31.0 41.0 72-73 35.178018257193706 36.0 35.0 39.0 31.0 40.5 74-75 34.813669303153354 35.0 34.0 37.5 31.0 40.0 76-77 34.12406146653031 35.0 34.0 37.0 29.5 39.0 78-79 34.14976822803647 35.0 34.0 37.0 30.0 39.0 80-81 33.93957107467952 35.0 34.0 36.0 30.0 38.5 82-83 33.69170407343361 35.0 34.0 36.0 30.0 37.0 84-85 33.45562269195271 35.0 34.0 35.5 30.0 37.0 86-87 33.257015748830625 35.0 34.0 35.0 30.0 36.5 88-89 33.06168773276723 35.0 34.0 35.0 29.5 36.0 90-91 32.90945561344014 35.0 34.0 35.0 29.0 36.0 92-93 32.7557772673393 35.0 34.0 35.0 29.0 36.0 94-95 32.60490821359243 35.0 34.0 35.0 29.0 35.5 96-97 32.44690467939941 35.0 33.5 35.0 29.0 35.0 98-99 32.256249320131836 35.0 33.0 35.0 29.0 35.0 100-101 31.503951751228247 34.5 32.0 35.0 26.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4196494041675436 1101 2 0.40107910908882616 1101 3 0.3698415352988178 1101 4 0.24530029861927716 1101 5 0.2691358707043534 1101 6 0.30946571867726647 1101 7 0.3107133981726875 1101 8 0.33244479710590724 1101 9 0.4471524182812274 1101 10-11 0.397392968261741 1101 12-13 0.389393891083742 1101 14-15 0.5281739669392564 1101 16-17 0.5294891377788318 1101 18-19 0.4558085768311386 1101 20-21 0.40791922732745434 1101 22-23 0.44745549886909686 1101 24-25 0.4116097519462656 1101 26-27 0.4061772627398099 1101 28-29 0.46432259680918264 1101 30-31 0.43263289931467597 1101 32-33 0.4231054629052977 1101 34-35 0.3689698199532785 1101 36-37 0.2905041833915689 1101 38-39 0.238205187625951 1101 40-41 0.38146298130362766 1101 42-43 0.3695245446900728 1101 44-45 0.35071779023430594 1101 46-47 0.37796385289136936 1101 48-49 0.3671646288348427 1101 50-51 0.34345923580870164 1101 52-53 0.3551524677594884 1101 54-55 0.36658523200387094 1101 56-57 0.3381128523210677 1101 58-59 0.28705896630894756 1101 60-61 0.30523960524066496 1101 62-63 0.18977730980834906 1101 64-65 0.2091869756104643 1101 66-67 0.27221702478230725 1101 68-69 0.27263025484151626 1101 70-71 0.25832940963103823 1101 72-73 0.18976598728066563 1101 74-75 0.23500290059440232 1101 76-77 0.045713073170183804 1101 78-79 0.17966000043826114 1101 80-81 0.17825965322392534 1101 82-83 0.15428101717527198 1101 84-85 0.1261548828414476 1101 86-87 0.04227491897599123 1101 88-89 0.04948189018010396 1101 90-91 0.07632223645698843 1101 92-93 0.021779675625317907 1101 94-95 -0.04747219778329281 1101 96-97 -0.0835979972939711 1101 98-99 -0.12414426995008654 1101 100-101 0.013726731766841027 1102 1 0.3812407756635494 1102 2 0.33006982926817585 1102 3 0.32278860600811754 1102 4 0.2270126627713367 1102 5 0.23373390010981865 1102 6 0.29781660317740233 1102 7 0.2679243069982036 1102 8 0.300231309993336 1102 9 0.40519912091571086 1102 10-11 0.3648561232598695 1102 12-13 0.3768028694559291 1102 14-15 0.46388371740614076 1102 16-17 0.4047895760416864 1102 18-19 0.43013280582486146 1102 20-21 0.3783953109310758 1102 22-23 0.3387516294782742 1102 24-25 0.3448545828690399 1102 26-27 0.3623226263452395 1102 28-29 0.37823983536988237 1102 30-31 0.37909530734246744 1102 32-33 0.33515081802654834 1102 34-35 0.2510054834717934 1102 36-37 0.27433110117661386 1102 38-39 0.3006621651270507 1102 40-41 0.36778958229955805 1102 42-43 0.3434367071866191 1102 44-45 0.292288907682952 1102 46-47 0.3244042300743146 1102 48-49 0.3492511481630629 1102 50-51 0.3322752484349323 1102 52-53 0.36003649220720746 1102 54-55 0.34340153623797676 1102 56-57 0.3412245860934249 1102 58-59 0.33429302928986004 1102 60-61 0.3272249335293509 1102 62-63 0.34159832376452925 1102 64-65 0.2921635219274563 1102 66-67 0.22281239031515554 1102 68-69 0.25012831472700015 1102 70-71 0.3061048964853583 1102 72-73 0.3297191907689694 1102 74-75 0.3036531544129275 1102 76-77 0.1048014289345005 1102 78-79 0.2522596277691349 1102 80-81 0.18325034787199712 1102 82-83 0.19344533428624544 1102 84-85 0.17111529207871712 1102 86-87 0.1645471798757967 1102 88-89 0.13920317855266262 1102 90-91 0.1139450562292268 1102 92-93 0.19213452887166227 1102 94-95 0.1298281609759897 1102 96-97 0.09776339602854733 1102 98-99 0.09436519976900826 1102 100-101 0.3783693495676488 1103 1 0.35853924075633614 1103 2 0.30087904249079145 1103 3 0.30679578861420254 1103 4 0.1890496168627962 1103 5 0.2570650757804671 1103 6 0.24219430269074138 1103 7 0.26447757937993543 1103 8 0.27002364410999746 1103 9 0.337841388467055 1103 10-11 0.36809251489174244 1103 12-13 0.36268746008987307 1103 14-15 0.4931317756792879 1103 16-17 0.4413463459871281 1103 18-19 0.40396861534555484 1103 20-21 0.38970532874144226 1103 22-23 0.39719936580687687 1103 24-25 0.38101156723534757 1103 26-27 0.3867964582194574 1103 28-29 0.2930049194640958 1103 30-31 0.35161618088335445 1103 32-33 0.3595921140815008 1103 34-35 0.36066524566701474 1103 36-37 0.2514263423224392 1103 38-39 0.2791820947343453 1103 40-41 0.2992016529774304 1103 42-43 0.3160742011357698 1103 44-45 0.31861957493370596 1103 46-47 0.28499688639765 1103 48-49 0.35174080760011606 1103 50-51 0.4031036086243063 1103 52-53 0.39590298146379865 1103 54-55 0.3932610343843521 1103 56-57 0.4148578152076112 1103 58-59 0.39687676962239493 1103 60-61 0.3767634724292819 1103 62-63 0.3880508175150652 1103 64-65 0.3712283323849235 1103 66-67 0.3395767063811945 1103 68-69 0.4013555063910843 1103 70-71 0.40726954955032824 1103 72-73 0.3809800932706935 1103 74-75 0.40580246778360163 1103 76-77 0.24174478401580757 1103 78-79 0.4106399624102295 1103 80-81 0.4094484489027437 1103 82-83 0.4070651988281284 1103 84-85 0.3907227254571808 1103 86-87 0.34318492604431583 1103 88-89 0.3323276223583207 1103 90-91 0.3147125021365511 1103 92-93 0.3470306858705783 1103 94-95 0.3559836012384139 1103 96-97 0.3588110793581265 1103 98-99 0.3844030754667571 1103 100-101 0.6424540924618185 1104 1 0.28613021421594453 1104 2 0.26247225105322514 1104 3 0.25615947172966713 1104 4 0.16257671712896382 1104 5 0.1840599610954996 1104 6 0.22251339854372532 1104 7 0.26282415114425106 1104 8 0.21451459813748386 1104 9 0.32398930454800023 1104 10-11 0.3284455690582675 1104 12-13 0.3075978718961494 1104 14-15 0.3799220349213286 1104 16-17 0.3829986450449212 1104 18-19 0.32782970823637925 1104 20-21 0.3030359481824547 1104 22-23 0.31461178747655794 1104 24-25 0.2955230950932801 1104 26-27 0.3180420957583934 1104 28-29 0.316158339138795 1104 30-31 0.2898209407485197 1104 32-33 0.2764824627142133 1104 34-35 0.24693992296541012 1104 36-37 0.26068512434653 1104 38-39 0.2259595053640453 1104 40-41 0.22736707609082885 1104 42-43 0.24838342322783546 1104 44-45 0.277185154491832 1104 46-47 0.28391585759864313 1104 48-49 0.2654865861728055 1104 50-51 0.25791616240240955 1104 52-53 0.26045805199934335 1104 54-55 0.29826726090131217 1104 56-57 0.2954160680553741 1104 58-59 0.26837149676960337 1104 60-61 0.26360649563543603 1104 62-63 0.3097252108624815 1104 64-65 0.31186018466824095 1104 66-67 0.28242967613324765 1104 68-69 0.32596729800649626 1104 70-71 0.2691372635742155 1104 72-73 0.3383307913307334 1104 74-75 0.3254694700967917 1104 76-77 0.1574963694328133 1104 78-79 0.33158963653707474 1104 80-81 0.3631329350922812 1104 82-83 0.3141553810189279 1104 84-85 0.3919547697691357 1104 86-87 0.3864296957679514 1104 88-89 0.34742500361645057 1104 90-91 0.33298543972510686 1104 92-93 0.3460504808485041 1104 94-95 0.33419749632635387 1104 96-97 0.3565524633426165 1104 98-99 0.3621301022422472 1104 100-101 0.6875156026975624 1105 1 0.1629684612837039 1105 2 0.18201318957552104 1105 3 0.17160414509798727 1105 4 0.12799357431161695 1105 5 0.1404494706530528 1105 6 0.15814514074357788 1105 7 0.14295585428624946 1105 8 0.15872558066457998 1105 9 0.2018218295627534 1105 10-11 0.23461681074206808 1105 12-13 0.24978908889251272 1105 14-15 0.3413026116283291 1105 16-17 0.2843508585047019 1105 18-19 0.21053173651791468 1105 20-21 0.17575606555926981 1105 22-23 0.13454229519595629 1105 24-25 0.18401016282630422 1105 26-27 0.23514149788550043 1105 28-29 0.23476731598542244 1105 30-31 0.2827529378890077 1105 32-33 0.19525259504344916 1105 34-35 0.1902182094252396 1105 36-37 0.14262820104048757 1105 38-39 0.16605560075985437 1105 40-41 0.16501129073755294 1105 42-43 0.14087554842289052 1105 44-45 0.10286320087168832 1105 46-47 0.15111471721716896 1105 48-49 0.2220139232435372 1105 50-51 0.20214849311827976 1105 52-53 0.22094840963553963 1105 54-55 0.22173095686894584 1105 56-57 0.14520194487916172 1105 58-59 0.11361697675513938 1105 60-61 0.1449060553306154 1105 62-63 0.1748034271000165 1105 64-65 0.213504797015446 1105 66-67 0.23392233335957258 1105 68-69 0.21856311822616448 1105 70-71 0.24187179937245418 1105 72-73 0.2896816320534157 1105 74-75 0.2786280231149263 1105 76-77 0.08648143017390453 1105 78-79 0.23722351625794147 1105 80-81 0.22633197564135088 1105 82-83 0.27794772383843025 1105 84-85 0.2701548838676402 1105 86-87 0.26941697164815537 1105 88-89 0.2974935323859569 1105 90-91 0.2461895792795019 1105 92-93 0.2825753288533903 1105 94-95 0.29342525019067267 1105 96-97 0.25640135845727485 1105 98-99 0.260777750732899 1105 100-101 0.5390778658010937 1106 1 0.05441559260248141 1106 2 0.07097856989865647 1106 3 0.06699270570805282 1106 4 0.056046636467542044 1106 5 0.09398776414436583 1106 6 0.13640666332068463 1106 7 0.13408677801398028 1106 8 0.060223932331076924 1106 9 0.18127299267091246 1106 10-11 0.1448555431355274 1106 12-13 0.17045715609584278 1106 14-15 0.2062389926511159 1106 16-17 0.17672276557568267 1106 18-19 0.1298927734406803 1106 20-21 0.08473588710431557 1106 22-23 0.09136089793811664 1106 24-25 0.09607320849720935 1106 26-27 0.07609596612054048 1106 28-29 0.10640045238980633 1106 30-31 0.1200312777864525 1106 32-33 0.06961800258198281 1106 34-35 0.07823873317728669 1106 36-37 0.07324692153067502 1106 38-39 0.05069222852819166 1106 40-41 0.08033196217073169 1106 42-43 0.07977232227720776 1106 44-45 0.022641503742200086 1106 46-47 0.08098059872437346 1106 48-49 0.1137081967423228 1106 50-51 0.11624961009495394 1106 52-53 0.14684003609942664 1106 54-55 0.1617546016197764 1106 56-57 0.11532375101880632 1106 58-59 0.04478857642351386 1106 60-61 0.06368383900927199 1106 62-63 0.014674870795140293 1106 64-65 0.08890344165244102 1106 66-67 0.1117315641901584 1106 68-69 0.14183613544841478 1106 70-71 0.16782498640379373 1106 72-73 0.20325443823892897 1106 74-75 0.16896069894263377 1106 76-77 -0.04089363293364556 1106 78-79 0.18764691621402108 1106 80-81 0.1678504359268871 1106 82-83 0.1595616383528622 1106 84-85 0.19109951588058038 1106 86-87 0.18781580686000154 1106 88-89 0.17495694246896676 1106 90-91 0.14779941915396222 1106 92-93 0.18668066522256765 1106 94-95 0.2187652832335516 1106 96-97 0.19482657098346579 1106 98-99 0.17155433823798916 1106 100-101 0.5421451312000194 1107 1 -6.93558750519685E-4 1107 2 0.02242481297197685 1107 3 0.01344471889792942 1107 4 0.027102564916056338 1107 5 0.027009523593150675 1107 6 0.020342146065090105 1107 7 0.036400437640878636 1107 8 0.06930302757928075 1107 9 0.04880455635410641 1107 10-11 0.10748640782335883 1107 12-13 0.09274496199302007 1107 14-15 0.12384471537290409 1107 16-17 0.0923303524889647 1107 18-19 0.06754118957371702 1107 20-21 0.01382608294082388 1107 22-23 -0.012448062608150678 1107 24-25 -0.02484251225724421 1107 26-27 0.015148680685719285 1107 28-29 -0.0012467369923783167 1107 30-31 -0.057879883292891066 1107 32-33 -0.051589778386613716 1107 34-35 -0.11546281974835182 1107 36-37 -0.10308629914366918 1107 38-39 -0.17168232633697755 1107 40-41 -0.23203332624933637 1107 42-43 -0.0579920546067072 1107 44-45 -0.06089113228432552 1107 46-47 -0.03703105088811043 1107 48-49 -0.04160001908905997 1107 50-51 0.03732420018046412 1107 52-53 0.06155736996920069 1107 54-55 0.06267529519136872 1107 56-57 0.02071048466572023 1107 58-59 0.03366649782893205 1107 60-61 0.004728459234819127 1107 62-63 0.0034236166218946096 1107 64-65 0.027852172685499 1107 66-67 0.11175433530951295 1107 68-69 0.0718908862958898 1107 70-71 0.0737487016470908 1107 72-73 0.06184652603280938 1107 74-75 0.017863703148208288 1107 76-77 -0.20891040915031311 1107 78-79 -0.005910644172104185 1107 80-81 0.028083016009496475 1107 82-83 -0.015504626357902396 1107 84-85 0.04179537123991395 1107 86-87 0.1445880764762606 1107 88-89 0.08531531880394994 1107 90-91 0.061713661115234686 1107 92-93 0.09895416892446462 1107 94-95 0.11224650962452642 1107 96-97 0.07755978360948035 1107 98-99 0.059980510196105286 1107 100-101 0.40542029934754 1108 1 -0.11585983070807515 1108 2 -0.10623213993785896 1108 3 -0.13570177085647828 1108 4 -0.05511982998198306 1108 5 -0.11714314979484186 1108 6 -0.03899313226717993 1108 7 -0.09618523546841118 1108 8 -0.06599353294741661 1108 9 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60-61 -0.6385001685611158 2308 62-63 -0.7894984662191433 2308 64-65 -0.6476166828133998 2308 66-67 -0.7704357708729859 2308 68-69 -0.7707308285986869 2308 70-71 -1.1337388161016975 2308 72-73 -0.9045758080855393 2308 74-75 -0.8208414892352849 2308 76-77 -1.1696249050719132 2308 78-79 -0.9898021753230068 2308 80-81 -0.9505093662138151 2308 82-83 -1.008839270149437 2308 84-85 -1.0564598599831427 2308 86-87 -1.1385454151941872 2308 88-89 -1.1606494090602837 2308 90-91 -1.0509919227408915 2308 92-93 -0.9548192754007729 2308 94-95 -1.0100220894397651 2308 96-97 -1.0284304959828994 2308 98-99 -1.2410327595521053 2308 100-101 -1.1156455110201442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 6.0 11 57.0 12 213.0 13 791.0 14 2420.0 15 5064.0 16 8657.0 17 14499.0 18 26690.0 19 45671.0 20 41405.0 21 36656.0 22 36025.0 23 38514.0 24 44084.0 25 54690.0 26 69220.0 27 89347.0 28 112048.0 29 141531.0 30 180226.0 31 231628.0 32 303273.0 33 412682.0 34 600776.0 35 961868.0 36 1772962.0 37 3536706.0 38 4384181.0 39 1114640.0 40 942.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 24.190271644286828 30.77594817379399 23.352626942220176 21.681153239699007 2 23.636099965213635 29.13555630117243 26.60954590367211 20.618797829941833 3 22.90914817915138 26.789348337120657 27.550955395932146 22.75054808779582 4 24.00955344814293 26.963174015742513 27.107337861233187 21.919934674881368 5 23.981146223378015 28.094831297431806 25.870161635240667 22.05386084394951 6 23.851001775226894 27.02941347983721 25.03746283854631 24.082121906389585 7 24.113381824054045 27.079092918493195 26.031030584815586 22.77649467263717 8 23.36198031438225 26.922933509173873 26.124915472061204 23.59017070438267 9 23.00052174624909 26.466615809724388 26.497756575236313 24.03510586879021 10-11 23.395662644218355 26.94626724854506 26.31652707349021 23.34154303374638 12-13 23.22434250622632 26.785874803385568 26.648583319470198 23.341199370917916 14-15 23.040784660379916 26.775034147605126 26.757045677047763 23.427135514967194 16-17 23.21883005157785 26.97664053429912 26.5519790481269 23.25255036599613 18-19 22.929734854219443 26.80164713132081 26.89914513236823 23.369472882091515 20-21 22.75444451546847 26.85284050320898 26.967990545206604 23.424724436115945 22-23 22.790106053826495 26.774049390109194 27.280614253176736 23.155230302887574 24-25 22.787095709737507 26.46523504654504 27.704221182280925 23.043448061436532 26-27 22.79369814077785 26.478184081945283 27.775162271213848 22.95295550606302 28-29 22.80849403454235 26.557546424482208 27.76841265222038 22.865546888755066 30-31 22.855747172417995 26.47443254824891 27.71834119020527 22.951479089127826 32-33 22.816102249929067 26.519764678704117 27.716346666038667 22.947786405328145 34-35 22.900206147241782 26.586440821471385 27.687963922410365 22.82538910887647 36-37 22.88021311694181 26.534907515500993 27.588941474705543 22.995937892851657 38-39 22.85866059523369 26.594455555966746 27.58996127695222 22.956922571847347 40-41 22.960548569371532 26.773477318798715 27.312925174836057 22.953048936993696 42-43 23.025485689181632 26.711450631219662 27.224485258691494 23.038578420907214 44-45 22.914206129676085 26.83414666781101 27.2323791148785 23.01926808763441 46-47 23.024812156842778 26.967343047936165 27.01872584334878 22.98911895187228 48-49 23.012356358575648 26.906006894564083 26.967440342619913 23.11419640424036 50-51 22.96747454629664 26.892781005632955 27.015640892794462 23.124103555275948 52-53 23.010607625513476 26.999835710208508 26.919386980398492 23.070169683879527 54-55 23.04195433162413 26.90666303322538 26.90614437069624 23.14523826445425 56-57 22.96253479376101 26.925691667219496 26.99313882995819 23.118634709061308 58-59 23.053371359735447 27.017900984516825 26.907653575649842 23.021074080097883 60-61 23.056789598045118 26.94184015220075 26.842614444941614 23.158755804812515 62-63 22.99927415312257 27.0055655269553 26.893096408389656 23.10206391153247 64-65 23.04883093515095 27.095118883008844 26.817294612528414 23.03875556931179 66-67 23.033243030019616 26.99851101863035 26.824965908466474 23.14328004288356 68-69 23.002969134791794 27.07222997568733 26.844806810688603 23.079994078832275 70-71 23.073682245068447 27.184240201852226 26.763839720112898 22.978237832966425 72-73 23.058589781115217 27.120758252196364 26.70489622654205 23.11575574014637 74-75 22.9834230405921 27.17641412028443 26.796395654324463 23.04376718479901 76-77 23.054289167714217 27.255739397416278 26.712767116912374 22.97720431795713 78-79 23.05489241575181 27.174713936961048 26.723898023554266 23.04649562373288 80-81 22.972343671925504 27.2273973708829 26.817054727468687 22.98320422972291 82-83 23.004181798601298 27.33898628032086 26.728700983930143 22.9281309371477 84-85 22.96218032254779 27.28679870084858 26.72530099421292 23.025719982390708 86-87 22.929551170348308 27.352261390287914 26.790020537640117 22.928166901723657 88-89 23.017231116595717 27.42110160035642 26.6867254269002 22.874941856147668 90-91 22.980306015222435 27.332485130903944 26.683752204102667 23.003456649770957 92-93 22.91708917827437 27.411333540729803 26.75275687838384 22.91882040261199 94-95 22.99819071743349 27.47662592387275 26.676887703151774 22.848295655541982 96-97 22.934718891945614 27.47508604983852 26.6110218578873 22.979173200328564 98-99 22.882179164975888 27.524941323140446 26.69594636672374 22.896933145159927 100-101 22.950175067337028 27.633622783464297 26.56151166612679 22.85469048307188 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25033.0 1 14271.0 2 2581.5 3 1426.0 4 1133.5 5 977.5 6 903.5 7 904.5 8 885.5 9 902.0 10 896.5 11 932.5 12 1199.5 13 1763.0 14 2341.0 15 2751.5 16 6015.0 17 6686.5 18 4714.0 19 5882.0 20 8081.0 21 11542.5 22 16671.0 23 23934.0 24 32911.5 25 44193.0 26 58201.5 27 75755.0 28 95992.5 29 116940.0 30 139292.5 31 164628.5 32 195428.5 33 227327.5 34 255242.0 35 287392.0 36 325026.5 37 365678.5 38 403532.0 39 435332.0 40 464975.0 41 494245.0 42 529125.0 43 560937.0 44 581511.5 45 588573.0 46 583604.5 47 574185.0 48 569875.5 49 563911.0 50 550705.5 51 530273.5 52 485478.5 53 434733.5 54 403347.0 55 379614.0 56 346026.5 57 308976.5 58 282575.5 59 259672.0 60 230354.0 61 202466.0 62 179150.0 63 156457.0 64 135133.0 65 113650.0 66 93652.5 67 75010.0 68 58473.5 69 45129.0 70 33797.0 71 25139.0 72 18974.5 73 14824.0 74 11872.0 75 8758.5 76 6227.5 77 4766.5 78 3694.0 79 2369.0 80 1170.5 81 562.5 82 297.5 83 180.5 84 96.0 85 51.5 86 32.0 87 21.0 88 13.5 89 7.0 90 5.5 91 2.5 92 1.0 93 1.5 94 2.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07423879997801994 2 0.002726481607954093 3 9.882619710064964E-4 4 0.002607329455421395 5 0.0028736695610827204 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.504475074491122E-6 12-13 1.226566276071893E-4 14-15 0.0 16-17 7.569666160900824E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.7872822879904724E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.42948715792118E-4 42-43 9.812530208575142E-5 44-45 0.0012370797012953663 46-47 3.5395198252360336E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.2053700893893466E-5 56-57 5.2567126117366835E-5 58-59 1.1564767745820703E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.121432023837159E-4 70-71 3.5044750744911216E-5 72-73 0.0016821480357557387 74-75 9.74244070708532E-4 76-77 5.396891614716328E-4 78-79 8.30560592654396E-4 80-81 0.001745228587096579 82-83 3.2591618192767436E-4 84-85 3.5044750744911216E-5 86-87 7.008950148982243E-5 88-89 3.9600568341749675E-4 90-91 3.1189828162970986E-4 92-93 7.885068917605025E-4 94-95 0.003441394523150282 96-97 9.46208270112603E-5 98-99 9.49712745187094E-4 100-101 0.0017347151618731054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4267472E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.92007238480065 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3197740883683 38.80329089949013 2 11.880625426719133 11.624021117036062 3 3.585927618669714 5.262715160159346 4 1.4582676684327207 2.853542395845727 5 0.7398955734308901 1.8097872504716364 6 0.4276444847698807 1.2552239489942003 7 0.2967116916226497 1.016061020211767 8 0.2045120839050016 0.8003796758559282 9 0.157666539295985 0.6941752663518542 >10 1.5401599219468574 16.6936661023415 >50 0.26247179455791747 8.835031566089189 >100 0.1259682258531631 9.738400350453762 >500 2.3157700897092335E-4 0.07072910158433578 >1k 1.0031398240366619E-4 0.15990946432344183 >5k 0.0 0.0 >10k+ 4.299143637012738E-5 0.3830666807909607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27715 0.1942530533790429 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16540 0.11592803546416632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.574564575980945E-4 0.0 0.0 0.0 0.0 2 3.6446540774707673E-4 7.008950148982244E-6 0.0 0.0 0.0 3 3.784833080450412E-4 7.008950148982244E-6 0.0 0.0 0.0 4 3.9951015849198793E-4 1.4017900297964488E-5 0.0 0.0 0.0 5 4.1352805878995243E-4 1.4017900297964488E-5 0.0 0.0 0.0 6 4.415638593858814E-4 1.4017900297964488E-5 0.0 0.0 0.0 7 4.555817596838459E-4 1.4017900297964488E-5 0.0 0.0 0.0 8 4.836175602797749E-4 1.4017900297964488E-5 0.0 7.008950148982244E-6 0.0 9 5.046444107267216E-4 2.8035800595928976E-5 0.0 7.008950148982244E-6 0.0 10-11 5.361846863971417E-4 2.8035800595928976E-5 0.0 7.008950148982244E-6 1.0513425223473367E-5 12-13 6.132831380359463E-4 3.504475074491122E-5 0.0 7.008950148982244E-6 1.4017900297964488E-5 14-15 7.920113668349936E-4 4.906265104287571E-5 0.0 1.0513425223473367E-5 1.4017900297964488E-5 16-17 0.0011214320238371591 5.957607626634907E-5 0.0 1.4017900297964488E-5 1.4017900297964488E-5 18-19 0.0012230618009974018 6.30805513408402E-5 0.0 1.4017900297964488E-5 1.752237537245561E-5 20-21 0.001321187103083153 6.30805513408402E-5 0.0 1.4017900297964488E-5 2.1026850446946733E-5 22-23 0.0014508526808393247 6.30805513408402E-5 0.0 1.752237537245561E-5 2.1026850446946733E-5 24-25 0.001636589859787354 6.30805513408402E-5 0.0 2.8035800595928976E-5 2.8035800595928976E-5 26-27 0.0018503628393313125 7.70984516388047E-5 0.0 3.504475074491122E-5 2.8035800595928976E-5 28-29 0.0020185776429068863 8.410740178778693E-5 0.0 3.504475074491122E-5 2.8035800595928976E-5 30-31 0.0022288461473763538 8.761187686227806E-5 0.0 3.504475074491122E-5 2.8035800595928976E-5 32-33 0.0025127086284101345 9.812530208575142E-5 0.0 3.504475074491122E-5 2.8035800595928976E-5 34-35 0.00277904873407146 9.812530208575142E-5 0.0 3.504475074491122E-5 2.8035800595928976E-5 36-37 0.003101460440924643 1.0162977716024254E-4 0.0 3.504475074491122E-5 2.8035800595928976E-5 38-39 0.0034343855730013 1.0513425223473367E-4 0.0 4.2053700893893466E-5 2.8035800595928976E-5 40-41 0.0040651910864097016 1.1564767745820703E-4 0.0 4.2053700893893466E-5 2.8035800595928976E-5 42-43 0.0045242773211680385 1.1915215253269816E-4 0.0 4.906265104287571E-5 2.8035800595928976E-5 44-45 0.0049167785295110445 1.261611026816804E-4 0.0 4.906265104287571E-5 3.504475074491122E-5 46-47 0.005666736195452144 1.3317005283066264E-4 0.0 5.2567126117366835E-5 3.504475074491122E-5 48-49 0.006546359439149416 1.3667452790515377E-4 0.0 5.607160119185795E-5 3.504475074491122E-5 50-51 0.007254263404196623 1.4368347805413602E-4 0.0 5.607160119185795E-5 3.504475074491122E-5 52-53 0.0079341315686479 1.5069242820311827E-4 0.0 6.30805513408402E-5 3.854922581940235E-5 54-55 0.008813754812345172 1.6821480357557387E-4 0.0 6.30805513408402E-5 5.2567126117366835E-5 56-57 0.00971440490648939 1.7522375372455612E-4 0.0 6.30805513408402E-5 5.607160119185795E-5 58-59 0.010601037100335645 1.7522375372455612E-4 0.0 6.658502641533132E-5 6.30805513408402E-5 60-61 0.011943251053865744 1.7872822879904724E-4 0.0 7.008950148982245E-5 6.658502641533132E-5 62-63 0.013257429206799915 1.8223270387353837E-4 0.0 7.008950148982245E-5 7.008950148982245E-5 64-65 0.014922054867183197 1.9274612909701171E-4 0.0 7.709845163880468E-5 7.008950148982245E-5 66-67 0.017192954715453443 1.9975507924599396E-4 0.0 7.709845163880468E-5 7.008950148982245E-5 68-69 0.020217316704739283 2.172774546184496E-4 0.0 7.709845163880468E-5 7.709845163880468E-5 70-71 0.025901575275563884 2.3129535491641406E-4 0.0 7.709845163880468E-5 9.111635193676918E-5 72-73 0.03210099168233868 2.3129535491641406E-4 0.0 8.410740178778693E-5 1.0162977716024254E-4 74-75 0.038591279520296236 2.3129535491641406E-4 0.0 8.410740178778693E-5 1.0513425223473367E-4 76-77 0.04698800179877696 2.4531325521437853E-4 0.0 8.410740178778693E-5 1.0513425223473367E-4 78-79 0.05722807796644003 2.6283563058683416E-4 0.0 9.111635193676918E-5 1.0513425223473367E-4 80-81 0.07025771629339803 2.663401056613253E-4 0.0 9.812530208575142E-5 1.0513425223473367E-4 82-83 0.08697756687379515 2.698445807358164E-4 0.0 9.812530208575142E-5 1.1915215253269816E-4 84-85 0.107370107332259 2.7334905581030754E-4 0.0 9.812530208575142E-5 1.1915215253269816E-4 86-87 0.13354152718855872 2.838624810337809E-4 0.0 9.812530208575142E-5 1.1915215253269816E-4 88-89 0.16539370114060853 2.8736695610827204E-4 0.0 1.0162977716024254E-4 1.261611026816804E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6385 0.0 86.82056 1 TCGACGG 7040 0.0 79.01477 2 CGACGGC 9070 0.0 61.380318 3 GACGGCG 11065 0.0 50.52696 4 ACGGCGC 11380 0.0 49.21116 5 CGGCGCG 12655 0.0 44.214638 6 ATCTCGA 9290 0.0 40.736366 1 TCTCGAG 10865 0.0 34.845974 2 CTCGAGG 12530 0.0 30.062902 3 TATCTCG 6495 0.0 27.938831 2 AGGGCGC 13680 0.0 27.777191 7 TCGAGGG 13885 0.0 27.538998 4 CGCGCCG 25860 0.0 26.927118 9 GGGCGCG 14105 0.0 26.80553 8 GGCGCGC 27415 0.0 25.573051 7 GCGCGCC 27960 0.0 25.05759 8 ATATCTC 8435 0.0 22.038223 1 CGAGGGC 17820 0.0 21.590832 5 GAGGGCG 18860 0.0 20.57611 6 ATCCATA 18330 0.0 17.206371 16-17 >>END_MODULE